Integrated anti-pollution strain cryopreservation tube device

Abstract

This invention relates to the field of microbial preservation technology and proposes an integrated anti-contamination cryopreservation tube device, comprising an outer tube with a pressing protrusion at its bottom; an inner tube with a diaphragm bottom; a reserved space between the inner and outer tubes; and sealing components at the top of both the inner and outer tubes. In summary, the cryopreservation process of this invention is essentially air-free, avoiding repeated opening of the cap during the cryopreservation process and reducing the probability of aerosol contamination; it also avoids bacterial cell rupture due to mechanical damage, thus improving the cryopreservation success rate.

Description

Technical Field

[0001] This utility model relates to the field of microbial preservation technology, and in particular to an integrated anti-contamination cryopreservation tube device for bacterial strains. Background Technology

[0002] Cryopreservation tubes are core tools in microbiology, molecular biology, biopharmaceuticals, and agriculture, used for the long-term preservation of microbial strains to maintain their genetic stability and biological activity. Their core principle is to utilize cryoprotectants to inhibit microbial metabolic activity through low temperatures (-80°C or liquid nitrogen), achieving long-term stable preservation.

[0003] Traditional cryopreservation tubes for microbial cultures are typically single-chambered, where the microbial culture and cryoprotectant are mixed and then sealed for preservation. However, this method has some limitations in its use.

[0004] 1. The revival of microbial cultures requires opening the lid multiple times, which increases the probability of aerosol contamination and poses a risk of operational contamination.

[0005] 2. Repeated freeze-thaw cycles or mechanical damage (such as pipetting) can cause bacterial cell rupture, reducing the success rate of resuscitation and easily damaging vulnerable strains.

[0006] 3. It requires separate preparation of resuscitation culture medium, etc., and has high environmental operation requirements. The process is complicated and not suitable for non-professional scenarios. Utility Model Content

[0007] In view of this, this utility model proposes an integrated anti-contamination cryopreservation tube device to solve the problem in the prior art that the cryopreserved bacterial strains are easily contaminated during the revival process, causing mechanical damage and resulting in a low revival success rate.

[0008] The technical solution of this utility model is implemented as follows:

[0009] This utility model provides an integrated anti-contamination cryopreservation tube device for bacterial cultures, including an outer tube with a pressing protrusion at the bottom; an inner tube with a diaphragm bottom at the bottom; a reserved space between the inner and outer tubes; and a sealing component at the top of the inner and outer tubes.

[0010] Based on the above technical solutions, preferably, the thickness of the diaphragm bottom is one-quarter to one-third of the thickness of the inner tube wall.

[0011] Based on the above technical solutions, preferably, a connecting rod is provided between the inner tube and the outer tube, and the connection is fixed by the connecting rod.

[0012] Based on the above technical solutions, preferably, the sealing component includes a sealing cap, which is threadedly connected to the port of the outer tube.

[0013] Based on the above technical solutions, preferably, the sealing assembly also includes a sealing membrane, which is attached to the openings of the inner tube and the outer tube.

[0014] Based on the above technical solutions, preferably, the top of the sealing cap is provided with multiple serrations that cooperate with the sealing film.

[0015] Based on the above technical solutions, preferably, a silicone gasket is also provided inside the sealing cover. The silicone gasket has an inner ring and an outer ring. The silicone gasket of the inner ring abuts against the opening of the inner tube, and the silicone gasket of the outer ring abuts against the opening of the outer tube.

[0016] The integrated anti-contamination cryopreservation tube device of this invention has the following advantages over the prior art:

[0017] 1. The process of reviving the microbial strain is basically free from contact with air, thus avoiding contact with air caused by multiple openings of the lid during the revival process and reducing the probability of aerosol contamination.

[0018] 2. The bacterial resuscitation process does not require pipetting, avoiding bacterial cell rupture due to mechanical damage and improving the resuscitation success rate, especially for fragile strains.

[0019] 3. No separate preparation of resuscitation culture medium is required, reducing environmental requirements and simplifying the resuscitation process; it can be applied to field use of agricultural microbial agents, activation of engineering bacteria in wastewater treatment, and other situations with poor environmental conditions. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a structural diagram of the cryopreservation tube device;

[0022] Figure 2 This is a structural diagram of a silicone pad;

[0023] Figure 3 This is a structural diagram of the sawtooth pattern;

[0024] In the diagram: 1-outer tube, 11-press outward protrusion, 12-reserved space, 13-support base; 2-inner tube, 21-diaphragm bottom; 3-connecting rod, 4-sealing cap, 41-serration, 42-silicone gasket. Detailed Implementation

[0025] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0026] See Figure 1 The integrated anti-contamination cryopreservation tube device of this utility model includes:

[0027] The outer tube 1 has a pressing protrusion 11 at its bottom and a support base 13 at its bottom. The outer tube 1 is made of medical-grade transparent polypropylene, which can withstand temperatures down to -196℃ and can be autoclaved. The outer tube 1 is primarily used to store cryoprotectants and resuscitation media, such as LB liquid medium and glycerol mixture. The capacity of the outer tube 1 is 1.5-2 mL. The pressing protrusion 11 is a flexible structure, allowing the user to manually press it. The support base 13 rests against the ground, suspending the pressing protrusion 11 in the air.

[0028] The inner tube 2 has a diaphragm bottom 21 at its bottom. The inner tube 2 is made of medical-grade polypropylene, with a wall thickness slightly thinner than the outer tube 1. The inner tube 2 is primarily used to store cryopreservation solutions for microorganisms, such as a mixture of bacterial culture, glycerol, and dimethyl sulfoxide. The capacity of the inner tube 2 is 0.2-0.5 mL. The diaphragm bottom 21 is part of the bottom of the inner tube 2 and is integrally formed with it. The thickness of the diaphragm bottom 21 is one-quarter to one-third of the wall thickness of the inner tube 2, making it relatively thin. When not compressed, the diaphragm bottom 21 completely separates the cryopreservation solution in the inner tube 2 from the culture medium in the outer tube 1. When compressed, due to its thinness, the diaphragm bottom 21 ruptures preferentially, releasing the microorganisms from the inner tube 2 into the resuscitation culture medium in the outer tube 1.

[0029] A reserved space 12 is provided between the inner tube 2 and the outer tube 1. The capacity of the reserved space 12 at the bottom is at least 0.2 mL. The pre-stored cryoprotectant and resuscitation culture medium are mainly located in the reserved space 12 at the bottom. In this invention, the capacity difference between the outer tube 1 and the inner tube 2 is large, and reserved spaces 12 are formed at the bottom and around the outer tube 1.

[0030] A connecting rod 3 is provided between the inner tube 2 and the outer tube 1, and the two tubes are fixedly connected by the connecting rod 3 to form an integrated structure, so that the positions of the inner tube 2 and the outer tube 1 are fixed and the shaking during transportation is avoided.

[0031] A sealing assembly is installed at the top of the inner tube 2 and the outer tube 1.

[0032] The sealing assembly includes a sealing cap 4, which is threaded to the opening of the outer tube 1. The sealing cap 4 seals the reagents contained in the inner tube 2 and the outer tube 1, preventing the reagents from spilling out of the inner tube 2 and the outer tube 1 during transportation due to shaking.

[0033] See Figure 2 To further improve the sealing effect, a silicone gasket 42 is also provided inside the sealing cap 4 to improve the sealing performance and prevent reagent leakage. The silicone gasket 42 has an inner ring and an outer ring. The silicone gasket 42 of the inner ring abuts against the opening of the inner tube 2, and the silicone gasket 42 of the outer ring abuts against the opening of the outer tube 1.

[0034] To prevent shaking during transportation and the resulting mixing of reagents in the inner tube 2 and outer tube 1, the sealing assembly in this invention also includes a sealing film. The sealing film is attached to the openings of the inner tube 2 and outer tube 1 (since the sealing film is only a single layer, it is not shown in the accompanying drawings of this invention). The sealing film is made of disposable easy-open aluminum film. Through the action of the sealing film, even if the sealing cap 4 has been opened, the inner tube 2 and outer tube 1 can be kept away from the outside air, avoiding contamination and maintaining a sterile environment in the inner tube 2 and outer tube 1.

[0035] In this invention, the top of the sealing cap 4 is also provided with a plurality of serrations 41 that cooperate with the sealing film. The height of the serrations 41 is 0.8-1.3mm. The serrations 41 are set in the groove at the top of the sealing cap 4, and the top of the serrations 41 is lower than the top of the sealing cap 4. With this setting, the serrations 41 will not be exposed and will not cause scratches to other experimental items.

[0036] During use, the bacterial strain and cryopreservation solution are injected into the inner tube 2, and the cryoprotectant and resuscitation medium are injected into the outer tube 1. After covering with the sealing film, the sealing cap 4 is tightened. The silicone gasket 42 on the sealing cap 4 presses the sealing film tightly, which can prevent the reagents in the inner tube 2 and the outer tube 1 from mixing due to shaking, and can also seal the inner tube 2 and the outer tube 1, so that the entire cryopreservation tube device can avoid external contamination and form a sterile environment. Then, the integrated anti-contamination bacterial strain cryopreservation tube device is frozen as a whole (-80℃ or liquid nitrogen).

[0037] When the bacterial strain needs to be revived, the cryopreservation tube device is first thawed. After thawing, the thumb is used to press the outer protrusion 11 until it is pressed down to the bottom 21 of the septum, causing the bottom 21 of the septum to break. The bacterial strain in the inner tube 2 then flows into the outer tube 1 and mixes with the culture medium. At this time, the sealing membrane is not opened, so the mixing process of the bacterial strain and the culture medium is always in a sterile environment. After the mixture is evenly mixed, the sealing cap 4 is inverted so that the top contacts the sealing membrane. When the sealing cap 4 is rotated, the serrations 41 can cut the sealing membrane, and then the mixture is poured out.

[0038] If the sealing membrane is not opened, the flow of bacteria from the inner tube 2 to the outer tube 1 will be obstructed due to the sealing effect. You can first use the serration 41 to puncture the sealing membrane, allowing the bacteria to flow smoothly into the outer tube 1. Then tighten the sealing cap 4, and then mix the bacteria and culture medium evenly. Although air enters during this process, the bacteria's contact time with air is short, and the probability of contamination is very low.

[0039] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An integrated anti-pollution bacterial strain cryo tube device, characterized by, include: The outer tube (1) has a pressing protrusion (11) at the bottom; Inner tube (2), with a diaphragm bottom (21) at the bottom of the inner tube (2); A reserved space (12) is provided between the inner tube (2) and the outer tube (1), and a sealing assembly is provided at the top of the inner tube (2) and the outer tube (1).

2. The integrated contamination-proof bacterial stock freezing tube device according to claim 1, wherein: The thickness of the diaphragm bottom (21) is one-quarter to one-third of the wall thickness of the inner tube (2).

3. The integrated contamination-proof bacterial stock freezing tube device according to claim 1, wherein: A connecting rod (3) is provided between the inner tube (2) and the outer tube (1), and the inner tube (2) and the outer tube (1) are fixedly connected by the connecting rod (3).

4. The integrated contamination-proof bacterial stock freezing tube device according to claim 1, wherein: The sealing assembly includes a sealing cap (4), which is threaded to the port of the outer tube (1).

5. The integrated contamination-proof bacterial stock freezing tube device according to claim 4, wherein: The sealing assembly also includes a sealing membrane, which is attached to the openings of the inner tube (2) and the outer tube (1).

6. The integrated contamination-proof bacterial stock freezing tube device according to claim 5, wherein: The top of the sealing cap (4) is provided with a plurality of serrations (41) that cooperate with the sealing film.

7. The integrated contamination-proof bacterial stock freezing tube device according to claim 4, wherein: The sealing cover (4) is also provided with a silicone gasket (42). The silicone gasket (42) has an inner ring and an outer ring. The silicone gasket (42) of the inner ring abuts against the opening of the inner tube (2), and the silicone gasket (42) of the outer ring abuts against the opening of the outer tube (1).

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