A device for detecting the abundance of bacteria in a biological deodorization tower

Abstract

This invention discloses a device for detecting the abundance of microorganisms in a biological deodorization tower, solving the technical problem of difficulty in detecting the abundance of microorganisms in deodorization towers. The device includes: a reactor for mixing and reacting a liquid sample of microorganisms with a fluorescent agent; a sample inlet pipe assembly connected to the inlet of the reactor for delivering the liquid sample of microorganisms into the reactor; a fluorescent agent pipe assembly connected to the inlet of the reactor for delivering the fluorescent agent into the reactor; and an infrared detection mechanism disposed on the reactor. The ATP of the microorganisms in the liquid sample of microorganisms can react with the fluorescent agent to emit fluorescence, and the infrared detection mechanism is used to detect the fluorescence intensity. This invention can detect the number of microorganisms in the liquid sample of microorganisms, allowing operators to calculate the abundance of microorganisms in the deodorization tower, facilitating adjustments to the biological deodorization tower.

Description

Technical Field

[0001] This utility model belongs to the field of microbial detection technology, specifically relating to a device for detecting the abundance of microbial species in a biological deodorization tower. Background Technology

[0002] Biological deodorization towers are environmental protection technologies that utilize the life activities of microorganisms to degrade and transform malodorous substances. Biological deodorization towers use pollutants in waste gas as a nutrient source for microorganisms, and transform them into harmless substances through biological metabolism, thereby achieving the purpose of deodorizing and purifying waste gas.

[0003] In biological deodorization towers, the number of bacteria inside is a crucial indicator affecting deodorization efficiency. Changes in deodorization conditions, such as variations in internal tower temperature, inlet gas concentration, and circulating liquid pH, can all impact the deodorization effect.

[0004] Under normal conditions, the number of bacteria in the water inside the deodorization tower is positively correlated with the amount of bacteria attached to the packing material inside the equipment (i.e., the thickness of the biofilm). The biofilm is in a state of equilibrium in the packing material of the deodorization tower. By detecting the number of bacteria in the water inside the deodorization tower, the abundance of bacteria inside the packing material can be correlated. Based on the abundance of bacteria and combined with the metabolic patterns of bacteria, the growth status of bacteria can be determined.

[0005] Therefore, there is an urgent need for a microbial abundance detection device to monitor whether the deodorization tower is operating normally and to provide clear direction for subsequent adjustments to the operation of the deodorization tower. Utility Model Content

[0006] To address all or part of the aforementioned problems, the purpose of this utility model is to provide a device for detecting the abundance of microorganisms in a biological deodorization tower. By detecting the number of microorganisms in a liquid sample, staff can calculate the abundance of microorganisms in the deodorization tower, thus enabling them to adjust the biological deodorization tower accordingly.

[0007] This utility model provides a device for detecting the abundance of bacteria in a biological deodorization tower, comprising:

[0008] A reactor for mixing and reacting liquid samples of bacterial strains with fluorescent reagents;

[0009] The sample inlet tube assembly is connected to the inlet end of the reactor and is used to deliver the bacterial liquid sample into the reactor.

[0010] A fluorescent reagent tube assembly is connected to the inlet end of the reactor and is used to deliver fluorescent reagent into the reactor;

[0011] An infrared detection mechanism is installed on the reactor;

[0012] In this process, the ATP of the bacterial strain in the liquid sample can react with the fluorescent agent to emit fluorescence, and the infrared detection mechanism is used to detect the fluorescence intensity.

[0013] Optionally, the reactor includes:

[0014] The reaction vessel, wherein the sample inlet tube assembly and the fluorescent reagent tube assembly are respectively connected to the inlet end of the reaction vessel;

[0015] An exhaust pipe assembly is connected to the top of the reaction vessel;

[0016] A drain pipe assembly is connected to the bottom of the reaction vessel;

[0017] A filter membrane is placed inside the reaction vessel and is used to filter out the bacterial strains from the liquid sample.

[0018] Optionally, the inlet end of the reaction vessel is tangentially positioned so that the bacterial liquid sample and fluorescent agent can be rotary-cut into the reaction vessel.

[0019] Optionally, the reactor further includes:

[0020] An ultrasonic disruption generator is installed on the reaction vessel to promote the lysis of bacteria on the inner wall of the reaction vessel and the filter membrane.

[0021] Optionally, the exhaust pipe assembly includes:

[0022] An exhaust pipe is connected to the top of the reaction vessel;

[0023] An exhaust control valve is connected to the exhaust pipe.

[0024] Optionally, the drain pipe assembly includes:

[0025] A drain pipe is connected to the bottom of the reaction vessel;

[0026] A pressure sensor is connected to the drain pipe;

[0027] A drain control valve is connected to the drain pipe.

[0028] Optionally, the sample inlet tube assembly includes:

[0029] The inlet pipe is connected to the inlet end of the reactor;

[0030] The sample channel is connected to the liquid inlet channel;

[0031] A metering pump is connected to the inlet pipe;

[0032] A one-way valve, connected to the inlet pipe, is used to restrict the flow of the bacterial liquid sample only from the inlet pipe toward the reactor.

[0033] Optionally, a filter is connected to the sample pipeline to filter impurities in the bacterial liquid sample.

[0034] Optionally, the sample inlet tube assembly further includes:

[0035] The water inlet pipe has one end connected to the water inlet pipe and the sample pipe respectively via a three-way valve, and the other end is used to connect to an external pure water pipe.

[0036] Optionally, the fluorescent reagent tube assembly includes:

[0037] An air supply pipe is connected to the liquid inlet end of the reactor;

[0038] An air pump is connected to the air delivery pipeline;

[0039] A gas-liquid mixer is connected to the gas delivery pipe;

[0040] Storage tanks are used to store fluorescent reagents;

[0041] Add a pipe, one end of which is connected to the liquid inlet port of the gas-liquid mixer and the other end extends into the storage tank;

[0042] A flow regulating pump is connected to the added pipeline.

[0043] As can be seen from the above technical solution, the detection device for the abundance of bacteria in the biological deodorization tower provided by this utility model has the following advantages:

[0044] This detection device utilizes the characteristic that the ATP of bacteria reacts with fluorescent agents to emit fluorescence, and uses an infrared detection mechanism to detect the fluorescence brightness in order to determine the number of bacteria in the liquid sample. Based on this, staff can calculate the abundance of bacteria in the deodorization tower, so that they can adjust the biological deodorization tower accordingly.

[0045] Other features and advantages of this invention will be set forth in the following description. Attached Figure Description

[0046] The accompanying drawings are provided to further understand the technical solution of this utility model and constitute a part of the specification. They are used together with the embodiments of this utility model to explain the technical solution of this utility model, and do not constitute a limitation on the technical solution of this utility model.

[0047] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present utility model;

[0048] Figure 2This is a schematic diagram of the reactor structure in an embodiment of the present invention;

[0049] Figure 3 This is a schematic diagram of the sample inlet tube assembly in an embodiment of the present invention;

[0050] Figure 4 This is a schematic diagram of the fluorescent reagent tube assembly in an embodiment of this utility model;

[0051] Figure 5 This is a schematic diagram illustrating the usage state of this utility model embodiment, showing the steps for introducing liquid bacterial samples;

[0052] Figure 6 This is a schematic diagram illustrating the usage state of an embodiment of the present invention, showing the steps of adding fluorescent agents;

[0053] Figure 7 This is a schematic diagram illustrating the usage state of an embodiment of the present invention, showing the cleaning steps of the detection device.

[0054] Explanation of reference numerals in the attached figures:

[0055] 1. Reactor; 11. Reaction vessel; 12. Exhaust pipe assembly; 121. Exhaust pipe; 122. Exhaust control valve; 13. Drainage pipe assembly; 131. Drainage pipe; 132. Pressure sensor; 133. Drainage control valve; 14. Filter membrane; 15. Ultrasonic disruptor; 2. Sample inlet pipe assembly; 21. Inlet pipe; 22. Sample pipe; 23. Water inlet pipe; 24. Three-way valve; 25. Metering pump; 26. Check valve; 27. Filter; 3. Fluorescent reagent assembly; 31. Gas supply pipe; 32. Gas supply pump; 33. Gas-liquid mixer; 34. Storage tank; 35. Addition pipe; 36. Flow regulating pump; 4. Infrared detection mechanism. Detailed Implementation

[0056] To make the objectives, technical solutions, and advantages of this utility model clearer, the embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be arbitrarily combined with each other.

[0057] like Figures 1-7 The illustration shows an embodiment of the present invention, which discloses a device for detecting the abundance of microorganisms in a biological deodorization tower. The device includes a reactor 1, with a sample inlet tube assembly 2 and a fluorescent reagent tube assembly 3 connected to the inlet end of the reactor 1. The sample inlet tube assembly 2 is used to deliver a liquid microbial sample into the reactor 1, and the fluorescent reagent tube assembly 3 is used to deliver a fluorescent reagent into the reactor 1, so that the liquid microbial sample and the fluorescent reagent can mix and react within the reactor 1 to emit fluorescence. Simultaneously, an infrared detection mechanism 4 is connected to the reactor 1, and the infrared detection mechanism 4 is used to detect the fluorescence intensity.

[0058] The detection device in this embodiment reacts the ATP in the bacterial strain with a fluorescent agent to emit fluorescence, and uses an infrared detection mechanism 4 to detect the fluorescence intensity. Since the fluorescence intensity is directly proportional to the ATP concentration, that is, the more bacterial strains there are, the higher the fluorescence intensity. Therefore, the ATP content is determined by measuring the emitted fluorescence intensity. The ATP concentration is directly proportional to the intensity of the emitted fluorescence. Based on the fluorescence intensity, the number of bacterial strains in the bacterial liquid sample can be calculated, and the bacterial abundance in the deodorization tower can be deduced accordingly.

[0059] In this embodiment, ATP is adenosine triphosphate, which is the most direct energy source in living organisms. The fluorescent agent adopts the "luciferase-luciferin system", and ATP can react with luciferin and luciferase to produce fluorescence.

[0060] In one embodiment, such as Figure 1 , Figure 2 As shown, reactor 1 includes a reaction vessel 11, a sample inlet tube group 2 and a fluorescent agent tube group 3, which are respectively connected to the inlet end of the reaction vessel 11. The inlet end of the reaction vessel 11 is arranged tangentially so that the bacterial liquid sample and the fluorescent agent can be rotary cut into the reaction vessel 11, thereby promoting the mixing of the bacterial liquid sample and the fluorescent agent.

[0061] In one embodiment, such as Figure 1 , Figure 2 As shown, the top of the reaction vessel 11 is connected to an exhaust pipe assembly 12, and the bottom is connected to a drain pipe assembly 13. A filter membrane 14 is installed inside the reaction vessel 11. In this embodiment, the filter membrane 14 is a cellulose membrane filter, which is an artificial membrane with uniform pore size made mainly of cellulose acetate. This prevents bacteria exceeding the pore size limit from passing through, thereby filtering out the bacteria in the liquid sample.

[0062] In one embodiment, such as Figure 1 , Figure 2 As shown, reactor 1 also includes an ultrasonic disruption generator 15 disposed on reaction vessel 11. The ultrasonic disruption generator 15 can promote the lysis of bacteria on the inner wall of reaction vessel 11 and filter membrane 14, so that the bacterial liquid sample and fluorescent agent can be fully mixed.

[0063] In one embodiment, such as Figure 1 , Figure 2 As shown, the exhaust pipe assembly 12 includes an exhaust pipe 121 and an exhaust control valve 122. The exhaust pipe 121 is connected to the top of the reaction vessel 11, and the exhaust control valve 122 is connected to the exhaust pipe 121 and is used to control the opening and closing of the exhaust pipe 121.

[0064] In one embodiment, such as Figure 1 , Figure 2As shown, the drain pipe assembly 13 includes a drain pipe 131, a pressure sensor 132, and a drain control valve 133. The drain pipe 131 is connected to the bottom of the reaction tank 11. The pressure sensor 132 and the drain control valve 133 are respectively connected to the drain pipe 131. The pressure sensor 132 is used to detect the pressure inside the drain pipe 131, and the drain control valve 133 is used to control the opening and closing of the drain pipe 131.

[0065] In this embodiment, the main function of the pressure sensor 132 is to monitor the pressure change of the drain pipe 131. When the liquid in the reaction tank 11 is drained, the liquid pressure in the drain pipe 131 will become gas pressure and the pressure will drop sharply. The operator can determine whether the corresponding operation has been completed by the detection result of the pressure sensor 132.

[0066] In one embodiment, such as Figure 1 , Figure 3 As shown, the sample inlet pipe group 2 includes an inlet pipe 21, a sample pipe 22, and a water inlet pipe 23. The inlet pipe 21, sample pipe 22, and water inlet pipe 23 are connected by a three-way valve 24. The end of the inlet pipe 21 away from the three-way valve 24 is connected to the inlet end of the reaction tank 11. The end of the sample pipe 22 away from the three-way valve 24 is used to connect to the discharge pipe of the deodorization tower. The end of the water inlet pipe 23 away from the three-way valve 24 is used to connect to an external pure water pipe.

[0067] In one embodiment, such as Figure 1 , Figure 3 As shown, a metering pump 25 and a one-way valve 26 are installed on the inlet pipe 21. The metering pump 25 is used to monitor the inlet flow rate, and the one-way valve 26 is used to restrict the flow of the bacterial liquid sample only from the inlet pipe 21 toward the reactor 1. A filter 27 is connected to the sample pipe 22 to filter impurities in the bacterial liquid sample. In this embodiment, the filter 27 uses a multi-layer quartz sand filter head to improve the filtration effect.

[0068] In one embodiment, such as Figure 1 , Figure 4 As shown, the fluorescent reagent tube assembly 3 includes an air supply pipe 31, which is connected to the liquid inlet of the reaction vessel 11. An air supply pump 32 and a gas-liquid mixer 33 are connected to the air supply pipe, meaning that the air supply pump 32 can supply air into the gas-liquid mixer 33.

[0069] In one embodiment, such as Figure 1 , Figure 4As shown, the fluorescent agent tube assembly 3 also includes a storage tank 34 for storing fluorescent agents. The top of the storage tank 34 is connected to an addition pipe 35. One end of the addition pipe 35 is connected to the liquid inlet port of the gas-liquid mixer 33, and the other end extends to the bottom of the storage tank 34. At the same time, a flow regulating pump 36 is connected to the addition pipe 35, which means that the flow regulating pump 36 can deliver fluorescent agents into the gas-liquid mixer 33.

[0070] In one embodiment, the infrared detection mechanism 4 includes an infrared sensor probe, a signal transmission system, an information processing system, and a display system. The infrared sensor probe is located inside the reaction vessel 11 to detect fluorescence signals and transmits the detection information to the information processing system via the signal transmission system. The information processing system processes the information and sends it to the display system so that staff can view the fluorescence intensity detection results. Infrared detection technology is existing technology and will not be described in detail here.

[0071] In this embodiment, an electrical control system can also be added, and components such as metering pump 25, three-way valve 24, drain control valve 133, exhaust control valve 122, and air pump 32 are respectively connected to the electrical control system. The electrical control system is used to control each component of the detection device to facilitate the detection work of the staff.

[0072] The method of using the detection device in this embodiment is as follows:

[0073] (1) Injection of liquid bacterial samples

[0074] like Figure 5 As shown, the sample pipe 22 is connected to the discharge pipe of the deodorization tower. The three-way valve 24 and the port of the sample pipe 22 are opened, and the microbial liquid sample is drawn by the metering pump 25. The microbial liquid sample is filtered by the filter 27 to remove solid particulate impurities. Subsequently, the microbial liquid sample enters the reaction tank 11 at a constant flow rate, so that part of the microbial liquid sample is retained by the inner wall of the reaction tank 11 (that is, the microbial liquid sample remains on the inner wall of the reaction tank 11), and the other part is retained by the filter membrane 14. When the volume of the input microbial liquid sample reaches the preset value, the metering pump 25 is turned off, and the three-way valve 24 and the port of the sample pipe 22 are closed.

[0075] Next, air is pumped into the reaction vessel 11 by the air pump 32, and the air is used to push the bacterial liquid sample in the reaction vessel 11 out to the drain pipe 131 and discharged through the drain pipe 131. At this time, most of the bacteria in the bacterial liquid sample are enriched on the filter membrane 14 after filtration. At the same time, the infrared detection mechanism 4 detects the initial brightness in the reaction vessel 11.

[0076] (2) Addition of fluorescent agents

[0077] like Figure 6 As shown, air is supplied to the gas-liquid mixer 33 by the air pump 32, and fluorescent reagent is supplied to the gas-liquid mixer 33 by the flow regulating pump 36. After the air and fluorescent reagent are atomized by the gas-liquid mixer 33, they are spun into the reaction vessel 11 so that the atomized fluorescent reagent is in uniform contact with the bacterial liquid sample on the inner wall of the reaction vessel 11. Excess gas is discharged from the exhaust pipe 121.

[0078] Simultaneously, the ultrasonic disruption generator 15 is activated, causing the bacteria on the inner wall of the reaction vessel 11 and the filter membrane 14 to lyse and mix thoroughly with the fluorescent reagent attached thereto. When the amount of fluorescent reagent added reaches the preset value, the flow regulating valve and the exhaust control valve 122 are closed. At the same time, the infrared detection mechanism 4 detects the fluorescence intensity inside the reaction vessel 11 and displays the test results.

[0079] (3) Cleaning of the detection device

[0080] like Figure 7 As shown, the three-way valve 24 and the inlet pipe 23 are opened, and clean water is sprayed into the reaction tank 11 by the metering pump 25. At the same time, the ultrasonic crushing generator 15 is turned on to ultrasonically clean the residual liquid in the reaction tank 11. Immediately afterwards, gas is introduced into the reaction tank 11 by the air pump 32, and the liquid in the reaction tank 11 is forced out through the drain pipe 131 by the air.

[0081] Repeat this cleaning process 2-3 times. After the final cleaning is completed, close the drain control valve 133, open the exhaust control valve 122, and use the air pump 32 to fill the reaction tank 11 with gas and dry the reaction tank 11 with air.

[0082] As can be seen from the above, this detection device can quickly and conveniently detect the number of bacteria in the liquid sample inside the deodorization tower. Staff can use the detection results to estimate the abundance of bacteria in the deodorization tower, so that they can adjust the biological deodorization tower accordingly.

[0083] It should be noted that, unless otherwise stated, the technical or scientific terms used in this utility model shall have the ordinary meaning as understood by those skilled in the art to which this utility model pertains.

[0084] Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly defined.

[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. These modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model. In particular, as long as there is no structural conflict, the various technical features mentioned in the embodiments can be combined in any way. This utility model is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A device for detecting the abundance of a bacterial species in a bio-deodorizing tower, characterized in that, include: Reactor (1) is used to mix and react liquid samples of bacterial strains and fluorescent reagents. The sample inlet tube assembly (2) is connected to the inlet end of the reactor (1) and is used to deliver the bacterial liquid sample into the reactor (1); A fluorescent agent tube assembly (3) is connected to the inlet end of the reactor (1) and is used to deliver fluorescent agents into the reactor (1); An infrared detection mechanism (4) is installed on the reactor (1); Among them, the ATP of the bacterial strain in the liquid sample can react with the fluorescent agent to emit fluorescence, and the infrared detection mechanism (4) is used to detect the fluorescence brightness.

2. The detection device of claim 1, wherein, The reactor (1) includes: The reaction vessel (11), the sample inlet tube assembly (2) and the fluorescent reagent tube assembly (3) are respectively connected to the inlet end of the reaction vessel (11); An exhaust pipe assembly (12) is connected to the top of the reaction vessel (11); A drain pipe assembly (13) is connected to the bottom of the reaction vessel (11); A filter membrane (14) is placed inside the reaction vessel (11) and is used to filter out the bacteria in the liquid sample.

3. The detection device of claim 2, wherein, The inlet end of the reaction vessel (11) is arranged tangentially so that the bacterial liquid sample and fluorescent agent can be rotary cut into the reaction vessel (11).

4. The detection device of claim 2, wherein, The reactor (1) further includes: An ultrasonic disruption generator (15) is installed on the reaction vessel (11) to promote the lysis of bacteria on the inner wall of the reaction vessel (11) and the filter membrane (14).

5. The detection device of claim 2, wherein, The exhaust pipe assembly (12) includes: An exhaust pipe (121) is connected to the top of the reaction vessel (11); An exhaust control valve (122) is connected to the exhaust pipe (121).

6. The detection device of claim 2, wherein, The drain pipe assembly (13) includes: A drain pipe (131) is connected to the bottom of the reaction vessel (11); A pressure sensor (132) is connected to the drain pipe (131); A drain control valve (133) is connected to the drain pipe (131).

7. The detection device of claim 1, wherein, The sample inlet tube assembly (2) includes: The liquid inlet pipe (21) is connected to the liquid inlet end of the reactor (1); The sample pipe (22) is connected to the liquid inlet pipe (21); A metering pump (25) is connected to the inlet pipe (21); A one-way valve (26) is connected to the inlet pipe (21) and is used to restrict the flow of the bacterial liquid sample only from the inlet pipe (21) toward the reactor (1).

8. The detection device of claim 7, wherein, The sample tube (22) is connected to a filter (27) to filter impurities in the bacterial liquid sample.

9. The detection device of claim 7, wherein, The sample inlet tube assembly (2) also includes: The water inlet pipe (23) is connected at one end to the water inlet pipe (23) and the sample pipe (22) respectively via a three-way valve (24), and at the other end to the external pure water pipe.

10. The detection device of claim 1, wherein, The fluorescent reagent tube assembly (3) includes: An air supply pipe (31) is connected to the liquid inlet end of the reactor (1); An air pump (32) is connected to the air supply pipe (31); A gas-liquid mixer (33) is connected to the gas delivery pipe (31); Storage tank (34) for storing fluorescent reagents; An addition conduit (35) is connected at one end to the liquid inlet port of the gas-liquid mixer (33) and extends into the storage tank (34) at the other end. A flow regulating pump (36) is connected to the addition conduit (35).

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