A closed dehydration treatment device for biological tissue samples

Abstract

This utility model relates to the technical field of biological sample dehydration devices, and more particularly to a closed dehydration treatment device for biological tissue samples. The technical solution includes a dehydration box with a cap snapped onto its top. Inside the dehydration box is a carrier, on which several mesh boxes are rotatably mounted. The mesh boxes are used to fix the tissue samples. An internal bracket is fixedly installed inside the dehydration box, and the carrier is inserted into the internal bracket. The internal bracket separates the multiple mesh boxes, allowing them to perform dehydration operations in independent zones. This utility model suspends multiple mesh boxes using the carrier, enabling batch dehydration operations. Different fixative immersions and different gradient dehydration treatments can be performed within the closed dehydration box. This not only controls the solvent volume but also reduces the impact of solvent evaporation on human health. Crucially, it solves the problem of tissue sample floating displacement during the vertical transfer of the mesh boxes, eliminating the need for sample replenishment and reprocessing, effectively improving the efficiency of dehydration treatment.

Description

Technical Field

[0001] This utility model relates to the technical field of biological sample dehydration devices, and in particular to a closed dehydration treatment device for biological tissue samples. Background Technology

[0002] Pretreatment steps for electron microscopy samples (plant tissue, animal tissue) or paraffin sections: glutaraldehyde / osmium tetroxide fixation - PBS buffer rinsing - dehydration with different gradient alcohol solutions or a general tissue fixative - dehydration with different gradient alcohol solutions. Currently, a dehydrator is generally used to fix multiple sample-laden meshes onto a connecting rod, then sequentially adding bottles containing the first solvent for immersion and washing, removing them and placing them into bottles containing the second solvent for further immersion. This operation is simple. However, in practice, it has been found that during the transfer of the meshes to multiple solvent bottles, tissue samples are prone to floating and shifting, easily leading to inconsistent sample labeling. To ensure the accuracy of subsequent experiments, the lost tissue samples need to be replenished and dehydrated again, significantly reducing the efficiency of the dehydration process.

[0003] To address the aforementioned issues, a closed-loop dehydration device is required. This device employs an internal support frame to house multiple mesh trays within a container. Through liquid inlet and outlet operations, the solvent inside the container is replaced, enabling batch processing. The dehydration process is conducted within the closed container, reducing solvent evaporation and resolving the issue of tissue sample floating during mesh tray transfer. This eliminates the need for sample replenishment and further processing, effectively improving dehydration efficiency. Therefore, this application proposes a closed-loop dehydration device for biological tissue samples that reduces sample loss. Utility Model Content

[0004] The purpose of this invention is to address the problem of tissue sample loss during floating and displacement in the prior art, and to propose a closed dehydration treatment device for biological tissue samples that can reduce sample loss.

[0005] The technical solution of this utility model: A closed dehydration treatment device for biological tissue samples, including a dehydration box, the top of which is fitted with a cap, and further comprising:

[0006] A carrier frame is disposed inside a dehydration box, and several mesh trays are rotatably mounted on the carrier frame for fixing tissue samples.

[0007] An internal bracket is fixedly installed inside the dehydration box, and the carrier is inserted into the internal bracket. The internal bracket is used to separate multiple carrier boxes and allow them to perform dehydration operations in independent sections.

[0008] Optionally, a first pulley and a second pulley are rotatably mounted in the groove at the top of the carrier, and an annular belt is sleeved on both the first pulley and the second pulley. A second spherical gear is fixedly mounted on the bottom of the second pulley and is coaxially arranged. A connecting sleeve coaxially arranged with the first pulley is rotatably mounted on the bottom of the carrier.

[0009] Optionally, a connector is fixedly installed at the bottom of the carrier, and a circular opening is cut in the middle of the built-in bracket. The connector is movably inserted into the circular opening, and a first sprocket is rotatably installed inside the connector. Both the second and third sprockets are meshed with the first sprocket.

[0010] Optionally, a support frame is fixedly installed at the bottom of the dehydration box, a servo motor is fixedly installed on the support frame, a connecting shaft is fixedly installed at the output end of the servo motor, and the top end of the connecting shaft is inserted into the circular port and engaged with the rotating shaft at the bottom end of the first spur gear.

[0011] Optionally, the top of the cover is chiseled with a liquid inlet, a liquid inlet sealing plug is inserted into the liquid inlet, a drain pipe is fixedly installed at the bottom of the dehydration box, and a ball valve is rotatably installed on the drain pipe.

[0012] Optionally, the bottom opening of the mesh carrier box is provided with a sealing plate, and an inner plate is fixedly installed on the upper surface of the sealing plate. The inner plate is embedded in the inside of the mesh carrier box and engaged with it. Several mesh holes are drilled on the top and side walls of the mesh carrier box.

[0013] Optionally, a locking rod is movably inserted through the carrier box. The nut at the bottom of the locking rod is embedded in the slot at the bottom of the sealing plate and engaged with it. The top of the locking rod passes through the sealing plate and the carrier box and is inserted into the matching connecting sleeve. A bolt is threaded on the wall of the connecting sleeve, and the top of the locking rod is fixed inside the connecting sleeve by the bolt.

[0014] Compared with the prior art, this application includes at least one of the following beneficial technical effects: by suspending and installing multiple carrier boxes by a carrier frame, batch dehydration operations can be carried out. Soaking and dehydration treatment are performed in a closed dehydration box, which not only controls the amount of solvent used and reduces the amount of solvent evaporation, but also solves the problem of tissue sample floating displacement when the carrier boxes are transferred up and down. There is no need to replenish the tissue samples and reoperate, which effectively improves the efficiency of dehydration treatment. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the internal structure of the dehydration box of this utility model;

[0017] Figure 3 This is a schematic diagram of the built-in bracket structure of this utility model;

[0018] Figure 4 This is a schematic diagram of the frame structure of this utility model;

[0019] Figure 5 This is a schematic diagram of the bottom structure of the carrier frame of this utility model;

[0020] Figure 6 This utility model Figure 4 Enlarged diagram of A in the middle;

[0021] Figure 7 This is a schematic diagram of the assembly of the carrier box of this utility model;

[0022] Figure 8 This is a schematic diagram of the sealing plate structure of this utility model.

[0023] Attached label: 1. Dehydration box;

[0024] 2. Cap; 21. Inlet sealing plug;

[0025] 3. Support frame;

[0026] 4. Servo motor;

[0027] 5. Drainage pipe;

[0028] 6. Built-in bracket; 61. Circular through-hole;

[0029] 7. Frame; 71. First pulley; 72. Second pulley; 73. Annular belt; 74. Connecting sleeve; 75. Insertion pipe; 76. First spur gear; 77. Second spur gear;

[0030] 8. Carrier box; 81. Sealing plate; 82. Locking rod; 83. Inner panel. Detailed Implementation

[0031] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments.

[0032] Example

[0033] like Figure 1 and Figure 2As shown, this utility model proposes a closed dehydration device for biological tissue samples, including a dehydration box 1. A cap 2 is snapped onto the top opening of the dehydration box 1, sealing the opening and ensuring that the tissue sample can be soaked and dehydrated within the enclosed space, effectively reducing solvent evaporation. An internal bracket 6 is fixedly installed inside the dehydration box 1, dividing the interior into multiple independent compartments. The bottom of the internal bracket 6 has an opening structure to ensure that the solvent inside the dehydration box 1 can flow within the multiple independent compartments. A carrier 7 is inserted into the internal bracket 6. When the cap 2 is fixed to the top of the dehydration box 1, the carrier 7 presses against the bottom of the cap 2, ensuring that the carrier 7 is securely installed on the internal bracket 6. Several mesh boxes 8 are rotatably mounted on the carrier 7, each mesh box... All boxes 8 are suspended within their respective designated areas. During dehydration, the rotating mesh box 8 ejects the solvent from the tissue sample. The ejected solvent is blocked by the built-in bracket 6, preventing it from splashing into adjacent mesh boxes 8. A drain pipe 5 is fixedly installed at the bottom of the dehydration box 1. By rotating the valve knob on the drain pipe 5, the ball valve installed inside the drain pipe 5 is opened, allowing the solvent inside the dehydration box 1 to be discharged. Then, by removing the inlet sealing plug 21 inserted into the cap 2, another solvent can be injected into the dehydration box 1 through the inlet, facilitating the replacement of different solvents. This allows the tissue sample in the mesh box 8 to be immersed in different solvents, solving the problem of floating and displacement during tissue sample transfer, avoiding insufficient sample volume, eliminating the need for replenishing the tissue sample, and effectively improving the efficiency of dehydration processing.

[0034] like Figures 2-6 As shown, to facilitate the simultaneous rotation of multiple screen holders 8 within the dehydration box 1, a first pulley 71 and a second pulley 72 are rotatably installed in a groove at the top of the frame 7. A ring belt 73 is fitted onto both the first pulley 71 and the second pulley 72. When the second pulley 72 rotates, it drives the first pulley 71 to rotate as well. A connecting sleeve 74 is rotatably installed below the frame 7. The connecting sleeve 74 is coaxially aligned with the matching first pulley 71. Since the screen holders 8 are fixed to the connecting sleeve 74, when the connecting sleeve 74 rotates with the first pulley 71, it drives the screen holders 8 to rotate, ensuring that multiple screen holders 8 can rotate synchronously within the dehydration box 1, thus improving dehydration efficiency.

[0035] Furthermore, to facilitate the rotation of the first pulley 71, a coaxially arranged second spur gear 77 is fixedly installed at the bottom of the second pulley 72, and a connector 75 is fixedly installed at the bottom of the carrier 7. When the connector 75 is inserted into the circular opening 61 cut into the inner bracket 6, the installation of the carrier 7 is completed. The first spur gear 76 is rotatably installed inside the connector 75, and multiple second spur gears 77 surround the outside of the first spur gear 76 and mesh with it. When the first spur gear 76 rotates, it can drive the multiple second spur gears 77 to rotate. At this time, the second pulley 72, which is coaxially arranged with the second spur gear 77, enters the rotation state, and under the transmission of the annular belt 73, it can drive the multiple first pulleys 71 to rotate.

[0036] Secondly, to facilitate the rotation of the first spur gear 76, a support frame 3 is installed at the bottom of the dehydration box 1. A servo motor 4 is fixedly installed on the support frame 3, and a connecting shaft is fixedly installed at the output end of the servo motor 4. The top end of the connecting shaft is inserted into the circular through-hole 61. When the carrier 7 is installed on the built-in bracket 6, the rotating shaft at the bottom of the first spur gear 76 engages with the connecting shaft (a slot is chiseled at the bottom of the rotating shaft, and a block is cut at the top of the connecting shaft. When the block is embedded in the slot, the connecting shaft and the rotating shaft are engaged). This facilitates assembly. When the servo motor 4 drives the connecting shaft to rotate, the first spur gear 76 can be driven to rotate.

[0037] like Figure 2 , Figure 7 and Figure 8 As shown, in order to facilitate the fixation of tissue samples inside the mesh carrier 8, a sealing plate 81 is provided at the bottom opening of the mesh carrier 8. When the tissue sample is placed inside the mesh carrier 8, the sealing plate 81 is placed over the bottom opening of the mesh carrier 8. At this time, the inner plate 83 at the top of the sealing plate 81 is embedded inside the mesh carrier 8 and engaged with it to prevent the sealing plate 81 from rotating relative to the mesh carrier 8. This ensures that the sealing plate 81 can stably seal the bottom opening of the mesh carrier 8, so that the tissue sample can be fixed inside the mesh carrier 8.

[0038] Furthermore, to facilitate the installation of the mesh carrier box 8, the locking rod 82 is inserted through the through hole on the sealing plate 81. After the top of the locking rod 82 passes through the mesh carrier box 8, the nut end of the locking rod 82 is embedded in the slot structure chiseled at the bottom of the sealing plate 81. At this time, the top of the locking rod 82 is inserted into the connecting sleeve 74. Through the bolts passing through the wall of the connecting sleeve 74, the top of the locking rod 82 can be fixed inside the connecting sleeve 74, ensuring that the mesh carrier box 8 can be firmly installed on the carrier frame 7. When the bottom end of the connecting sleeve 74 is in close contact with the top of the mesh carrier box 8, the mesh carrier box 8 and the sealing plate 81 can be clamped between the connecting sleeve 74 and the nut of the locking rod 82, which facilitates the installation of the mesh carrier box 8.

[0039] Secondly, several mesh holes are drilled on the top and side walls of the screen holder 8. When the screen holder 8 rotates, the solvent in the tissue sample is thrown out through the mesh holes, ensuring the dehydration effect.

[0040] In this embodiment, the tissue sample is first fixed and the mesh carrier 8 is installed. The tissue sample is placed inside the mesh carrier 8, and then the sealing plate 81 is snapped into the bottom opening of the mesh carrier 8 to ensure that the tissue sample can be fixed inside the mesh carrier 8. Then, the locking rod 82 is inserted through the mesh carrier 8. After the top end of the locking rod 82 is inserted into the connecting sleeve 74, the bolt on the wall of the connecting sleeve 74 is rotated. When the end of the bolt is threaded into the end of the locking rod 82 inserted into the connecting sleeve 74, multiple mesh carriers 8 can be fixed on the carrier frame 7, which facilitates the installation of the mesh carrier 8. Then, the carrier frame 7 is inserted into the internal bracket 6. When the insertion tube 75 is inserted into the circular through-hole 61 provided on the internal bracket 6, it can be ensured that the carrier frame 7 can be stably inserted into the internal bracket 6. Then, the cover 2 is fixed to the top opening of the dehydration box 1. At this time, the cover 2 covers the top of the carrier frame 7, which can prevent the carrier frame 7 from displacing vertically and effectively improve the firmness of the carrier frame 7 after installation.

[0041] After the carrier 7 is installed, solvent is injected into the dehydration box 1 through the inlet cut into the cap 2. As the solvent level rises, the carrier 8 and the tissue sample inside are immersed in the solvent. After immersion for a period of time, the valve knob installed on the drain pipe 5 is turned. When the internal channel of the drain pipe 5 is open, the solvent can be discharged. Repeating the above operation allows multiple different types of solvents to be injected into the dehydration box 1 in stages, ensuring that the tissue sample is immersed in different solvents. Since the tissue sample is immersed in a closed container, there is no need to transfer the carrier 8, which solves the problem of the carrier 8 being easily transferred to containers containing different solvents. The device effectively reduces the loss of tissue samples due to floating and displacement. After the soaking process is completed, the servo motor 4 drives the first spur gear 76 to rotate. Under the transmission action of the second spur gear 77, the second pulley 72, and the annular belt 73, multiple first pulleys 71 can be driven to rotate, ensuring that multiple mesh containers 8 can rotate synchronously. Under the action of centrifugal force, the solvent in the tissue sample can be shaken out, achieving the purpose of dehydration. This device places the tissue sample in a closed container, and there is no need to transfer the mesh container 8 during the soaking process, which can reduce the loss of tissue samples and eliminate the need for replenishment and reprocessing of tissue samples, effectively improving the efficiency of dehydration treatment.

[0042] The above specific embodiments are merely several optional embodiments of this utility model. Based on the technical solution of this utility model and the relevant teachings of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. A closed dehydration treatment device for biological tissue samples, comprising a dehydration box (1), wherein a cap (2) is snapped onto the top of the dehydration box (1), characterized in that, Also includes: A carrier (7) is disposed inside a dehydration box (1). Several mesh boxes (8) are rotatably mounted on the carrier (7). The mesh boxes (8) are used to fix tissue samples. Built-in bracket (6) is fixedly installed inside the dehydration box (1). The carrier (7) is inserted into the built-in bracket (6). The built-in bracket (6) is used to separate multiple carrier boxes (8) and allow them to perform dehydration operations in independent sections.

2. The closed dehydration treatment device for biological tissue samples according to claim 1, characterized in that, A first pulley (71) and a second pulley (72) are rotatably mounted in the groove at the top of the carrier (7). An annular belt (73) is sleeved on both the first pulley (71) and the second pulley (72). A second spur gear (77) is fixedly mounted on the bottom of the second pulley (72) and is coaxially arranged. A connecting sleeve (74) coaxially arranged with the first pulley (71) is rotatably mounted below the carrier (7).

3. The closed dehydration treatment device for biological tissue samples according to claim 2, characterized in that, The bottom of the carrier (7) is fixedly installed with a plug tube (75). A circular opening (61) is cut in the middle of the built-in bracket (6). The plug tube (75) is movably inserted into the circular opening (61). A first spur gear (76) is rotatably installed inside the plug tube (75). The second spur gear (77) is meshed with the first spur gear (76).

4. The closed dehydration treatment device for biological tissue samples according to claim 3, characterized in that, A support frame (3) is fixedly installed at the bottom of the dehydration box (1). A servo motor (4) is fixedly installed on the support frame (3). A connecting shaft is fixedly installed at the output end of the servo motor (4). The top end of the connecting shaft is inserted into the circular opening (61) and engages with the rotating shaft at the bottom end of the first spur gear (76).

5. The closed dehydration treatment device for biological tissue samples according to claim 1, characterized in that, The top of the cover (2) is chiseled with a liquid inlet, and a liquid inlet sealing plug (21) is inserted into the liquid inlet. A drain pipe (5) is fixedly installed at the bottom of the dehydration box (1), and a ball valve is rotatably installed on the drain pipe (5).

6. The closed dehydration treatment device for biological tissue samples according to claim 2, characterized in that, The bottom opening of the mesh carrier (8) is provided with a sealing plate (81), and an inner plate (83) is fixedly installed on the upper surface of the sealing plate (81). The inner plate (83) is embedded in the inside of the mesh carrier (8) and engaged with it. Several mesh holes are drilled on the top and side walls of the mesh carrier (8).

7. The closed-loop dehydration device for biological tissue samples according to claim 6, characterized in that, A locking rod (82) is movably inserted through the net box (8). The nut at the bottom of the locking rod (82) is embedded in the slot at the bottom of the sealing plate (81) and engaged with it. The top end of the locking rod (82) passes through the sealing plate (81) and the net box (8) and is inserted into the matching connecting sleeve (74). Bolts are threaded on the wall of the connecting sleeve (74). The top end of the locking rod (82) is fixed inside the connecting sleeve (74) by bolts.

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