A fluorescence detection device based on gene editing technology
By introducing a positioning structure consisting of a positioning groove and a holding plate into the fluorescence detection device, combined with the design of the driving component, the problem of damage caused by reagent tube shaking was solved, achieving the stability of the reagent tube and sufficient agitation of the reaction solution, thus promoting the effective execution of the gene editing reaction.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SINGLERA HEALTH TECH SHANGHAI LTD
- Filing Date
- 2025-06-16
- Publication Date
- 2026-07-31
AI Technical Summary
In existing gene editing fluorescence detection devices, reagent tubes are prone to contact with the device when the multi-well plate is shaken, leading to damage, and there is a lack of effective positioning structure.
The design incorporates a positioning groove and a pressure plate to position the reagent tube, while a drive assembly moves the movable plate up and down to ensure the stability of the reagent tube. Periodic oscillations also promote the full reaction of the reaction solution.
This effectively avoids damage to the reagent tubes from contact, ensures the stability of the reagent tubes during movement, and promotes the full execution of the gene editing reaction through periodic oscillation.
Smart Images
Figure CN224581400U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of gene detection technology, and in particular to a fluorescence detection device based on gene editing technology. Background Technology
[0002] The combination of gene editing technology and fluorescence detection methods has brought revolutionary breakthroughs to the field of molecular diagnostics. In recent years, gene editing tools, represented by the CRISPR-Cas system, have been widely used in the field of nucleic acid detection due to their high specificity and programmability.
[0003] In the prior art, a search revealed a Chinese patent disclosure entitled "A Fluorescence Detection Device Based on Gene Editing Technology," application number "202121779215.X." This patent mainly includes a box, with a placement base fixedly installed at one end inside the box. A support base is fixedly installed on the upper surface of the placement base. A perforated plate is movably connected to the upper surface of the support base. Two side grooves are provided on one outer surface of the perforated plate. The upper surface of the perforated plate has arranged pores. An isolation cover is fixedly installed at the upper end of the perforated plate. Heating plates are fixedly installed on the four end faces of the inner wall of the isolation cover. A sealing strip is fixedly installed at the lower end of the inner wall of the isolation cover. A heating tube is fixedly installed inside each sealing strip. A drive motor located on the inner wall of the support base is fixedly installed at the lower end of the perforated plate. One side of the drive motor is connected to the lower surface of the perforated plate via a connecting rod. Although the above patent includes an automatic plate-shaking structure and a protective structure... However, when setting pores to hold reagent tubes carrying the carrier material, the lack of a positioning structure for the reagent tubes means that the shaking caused by the up-and-down movement of the multi-well plate can lead to the reagent tubes bouncing up and down. This shaking can easily cause the reagent tubes to come into contact with the multi-well plate or the isolation cover, posing a risk of damage and resulting in the loss of the reaction solution inside the reagent tubes. Therefore, this invention provides a fluorescence detection device based on gene editing technology to solve the problems mentioned in the background art. Utility Model Content
[0004] The purpose of this invention is to provide a fluorescence detection device based on gene editing technology. The device is equipped with a positioning groove and a holding plate to position the reagent tube, which can maintain the stability of the reagent tube during movement and avoid the problem of the reagent tube being damaged by contact when the moving plate moves the reagent tube.
[0005] To achieve the above objectives, a fluorescence detection device based on gene editing technology is provided, comprising a movable plate and a base disposed below the movable plate, wherein a mounting shell is fixedly provided on the top of the movable plate, and positioning components are provided on all four sides of the mounting shell;
[0006] The positioning assembly includes two fixing plates fixed to the outside of the mounting shell, a fixing rod fixed between the fixing plates, a pressure plate sleeved on the outside of the fixing rod, and a spring sleeved on the outside of the pressure plate and located above the pressure plate. A placement seat is also fixed on the outside of the mounting shell and below the pressure plate.
[0007] The base is equipped with a drive component, which is used to drive the movable plate to move up and down reciprocally.
[0008] According to the aforementioned fluorescence detection device based on gene editing technology, one end of the holding plate is provided with a slot that connects to the neck of the reagent tube, and the top side of the placement seat is provided with a positioning slot.
[0009] According to the aforementioned fluorescence detection device based on gene editing technology, the driving component includes a push plate movable at the top of the base, a turntable disposed inside the base and located below the push plate, a curved plate rotating at the eccentric position of the turntable, and a control component for driving the turntable to rotate. The top of the push plate is fixedly connected to the bottom of the movable plate, and the top of the curved plate is rotatably connected to the bottom of the push plate.
[0010] According to the aforementioned fluorescence detection device based on gene editing technology, the control component includes a rotating shaft that rotates inside the base and a motor fixed to the outside of the base. The turntable is fixed to one end of the rotating shaft, and the other end of the rotating shaft is fixedly connected to the output shaft of the motor.
[0011] According to the aforementioned fluorescence detection device based on gene editing technology, guide rods are symmetrically fixed on the top of the base, and the movable plate slides on the outside of the guide rods.
[0012] According to the aforementioned fluorescence detection device based on gene editing technology, a guide plate is fixedly provided on the inner wall of the base, and the push plate slides inside the guide plate.
[0013] This utility model has the following beneficial effects:
[0014] 1. Compared with existing technologies, the positioning groove and the pressure plate are used to position the reagent tube, which can maintain the stability of the reagent tube during movement and avoid the problem of the reagent tube being damaged by contact when the moving plate drives the reagent tube to move.
[0015] 2. Compared with existing technologies, the driving component is set up to drive the movable plate to move up and down reciprocally, and the push plate drives the movable plate to move up and down along the guide rod, so that the reaction solution in the reagent tube is subjected to periodic oscillation, which promotes the gene editing reaction to proceed fully. Attached Figure Description
[0016] The present invention will be further described below with reference to the accompanying drawings and embodiments;
[0017] Figure 1This is a first-view structural schematic diagram of a fluorescence detection device based on gene editing technology according to this utility model;
[0018] Figure 2 This is a schematic diagram of the front cross-sectional structure of a fluorescence detection device based on gene editing technology according to this utility model;
[0019] Figure 3 This is a rear view schematic diagram of a fluorescence detection device based on gene editing technology according to this utility model;
[0020] Figure 4 This is a schematic diagram of the positioning component structure of a fluorescence detection device based on gene editing technology according to this utility model.
[0021] Legend:
[0022] 1. Movable plate; 2. Mounting shell; 3. Fixed plate; 4. Pressure plate; 5. Fixed rod; 6. Spring; 7. Placement seat; 8. Positioning groove; 9. Base; 10. Rotating shaft; 11. Guide plate; 12. Turntable; 13. Curved plate; 14. Push plate; 15. Guide rod; 16. Motor. Detailed Implementation
[0023] This section will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the accompanying drawings. The purpose of the drawings is to supplement the textual description with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present utility model, but they should not be construed as limiting the scope of protection of the present utility model.
[0024] Reference Figure 1-4 This utility model embodiment discloses a fluorescence detection device based on gene editing technology, which includes a movable plate 1 and a base 9 located below the movable plate 1. A mounting shell 2 is fixedly provided on the top of the movable plate 1. Positioning components are provided on all four sides of the mounting shell 2. The positioning components include two fixing plates 3 fixedly provided on the outside of the mounting shell 2, a fixing rod 5 fixedly provided between the fixing plates 3, a pressure plate 4 sleeved on the outside of the fixing rod 5, and a spring 6 sleeved on the outside of the pressure plate 4 and located above the pressure plate 4. A placement seat 7 is also fixedly provided on the outside of the mounting shell 2 and below the pressure plate 4.
[0025] Push the holding plate 4 upward to move it. After the reagent tube is stably placed inside the positioning groove 8, lower the holding plate 4 so that the slot engages with the bottleneck of the reagent tube. The spring 6 is set to have sufficient rigidity so that the spring 6 pushes the holding plate 4 to press against the reagent tube, which can maintain the stability of the reagent tube when it moves and avoid the problem of the reagent tube being damaged by contact when the movable plate 1 moves the reagent tube.
[0026] One end of the holding plate 4 has a slot that connects with the neck of the reagent tube. The top side of the placement seat 7 has a positioning groove 8. The reagent tube containing the gene editing reaction solution is inserted into the positioning groove 8 of the placement seat 7, and the neck of the reagent tube is locked into the slot of the holding plate 4 to ensure the stability of the reagent tube.
[0027] The base 9 is equipped with a drive assembly, which is used to drive the movable plate 1 to move up and down reciprocally. The drive assembly includes a push plate 14 movable at the top of the base 9, a turntable 12 located inside the base 9 and below the push plate 14, a curved plate 13 rotating at the eccentric position of the turntable 12, and a control assembly for driving the turntable 12 to rotate. The top of the push plate 14 is fixedly connected to the bottom of the movable plate 1, and the top of the curved plate 13 is rotatably connected to the bottom of the push plate 14. The control assembly includes a rotating shaft 10 rotating inside the base 9 and a motor 16 fixedly installed on the outside of the base 9. The turntable 12 is fixedly installed at one end of the rotating shaft 10, and the other end of the rotating shaft 10 is fixedly connected to the output shaft of the motor 16.
[0028] After the reagent tube is positioned, motor 16 is started, which drives the rotating shaft 10 to rotate, thereby driving the turntable 12 to rotate. The eccentric movement of the turntable 12 pushes the push plate 14 to move up and down along the guide plate 11 through the curved plate 13. The push plate 14 drives the movable plate 1 to move up and down along the guide rod 15, so that the reaction solution in the reagent tube is subjected to periodic oscillation, promoting the gene editing reaction to proceed fully.
[0029] Guide rods 15 are symmetrically fixed to the top of the base 9. The movable plate 1 slides on the outside of the guide rods 15. When the movable plate 1 moves up and down, it slides on the outside of the guide rods 15 to ensure the smoothness of the movement of the movable plate 1. A guide plate 11 is fixed on the inner wall of the base 9. The push plate 14 slides inside the guide plate 11. When the push plate 14 moves, it slides inside the guide plate 11 to ensure the smoothness of the movement of the push plate 14.
[0030] Working principle: Push the holding plate 4 upward to move it. After the reagent tube is stably placed inside the positioning groove 8, put down the holding plate 4 so that the slot engages with the bottleneck of the reagent tube. The spring 6 is set to have sufficient rigidity so that the spring 6 pushes the holding plate 4 to press against the reagent tube, which can maintain the stability of the reagent tube when it moves and avoid the problem of the reagent tube being damaged by contact when the movable plate 1 moves the reagent tube.
[0031] Then, motor 16 is started, which drives the rotating shaft 10 to rotate, thereby driving the turntable 12 to rotate. The eccentric motion of the turntable 12 pushes the push plate 14 to move up and down along the guide plate 11 through the curved plate 13. The push plate 14 drives the movable plate 1 to move up and down along the guide rod 15, so that the reaction solution in the reagent tube is subjected to periodic oscillation, promoting the gene editing reaction to proceed fully.
[0032] The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.
Claims
1. A fluorescence detection device based on gene editing technology, characterized by, It includes a movable plate (1) and a base (9) located below the movable plate (1). The top of the movable plate (1) is fixedly provided with a mounting shell (2), and the mounting shell (2) is provided with positioning components on all four sides. The positioning assembly includes two fixing plates (3) fixed to the outside of the mounting shell (2), a fixing rod (5) fixed between the fixing plates (3), a pressure plate (4) sleeved on the outside of the fixing rod (5), and a spring (6) sleeved on the outside of the pressure plate (4) and located above the pressure plate (4). A placement seat (7) is also fixed on the outside of the mounting shell (2) and below the pressure plate (4). The base (9) is equipped with a drive component, which is used to drive the movable plate (1) to move up and down reciprocally.
2. The fluorescence detection device based on gene editing technology according to claim 1, characterized in that, The pressure plate (4) has a slot at one end that is connected to the neck of the reagent tube, and the placement seat (7) has a positioning slot (8) on its top side.
3. The fluorescence detection device based on gene editing technology according to claim 2, characterized in that, The driving assembly includes a push plate (14) movable on the top of the base (9), a turntable (12) located inside the base (9) and below the push plate (14), a curved plate (13) rotating at the eccentric position of the turntable (12), and a control assembly for driving the turntable (12) to rotate. The top of the push plate (14) is fixedly connected to the bottom of the movable plate (1), and the top of the curved plate (13) is rotatably connected to the bottom of the push plate (14).
4. The fluorescence detection device based on gene editing technology according to claim 3, characterized in that, The control assembly includes a rotating shaft (10) that rotates inside the base (9) and a motor (16) fixed to the outside of the base (9). The turntable (12) is fixed to one end of the rotating shaft (10), and the other end of the rotating shaft (10) is fixedly connected to the output shaft of the motor (16).
5. The fluorescence detection device based on gene editing technology according to claim 4, characterized in that, The base (9) is symmetrically fixed with guide rods (15) on its top, and the movable plate (1) slides on the outside of the guide rods (15).
6. The fluorescence detection device based on gene editing technology according to claim 5, characterized in that, A guide plate (11) is fixedly provided on the inner wall of the base (9), and the push plate (14) slides inside the guide plate (11).