A sample tank for water bath heating in electrophoresis tanks
By laying meandering spiral heating strips and a double-layer stirring mechanism at the bottom of the electrophoresis tank sample tank, the problem of uneven heating was solved, temperature uniformity was achieved, and the reliability and efficiency of experimental results were improved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- KEDE CHEM IND SHUNDE
- Filing Date
- 2025-07-23
- Publication Date
- 2026-07-31
AI Technical Summary
Existing electrophoresis tanks suffer from uneven heating during the sample tank process, which affects the effectiveness and accuracy of electrophoresis experiments.
Heating strips are laid in a meandering spiral pattern at the bottom of the constant temperature sample tank, and a double-layer stirring mechanism is used. Temperature sensors monitor and controllers adjust the temperature to achieve uniform heating and flow of the water inside the constant temperature sample tank.
To ensure that the temperature of each part inside the sample tank is consistent, avoid experimental errors caused by temperature deviations, improve the repeatability and comparability of experimental data, reduce energy consumption, extend the service life of the sample, and reduce operational risks.
Smart Images
Figure CN224581464U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of electrophoresis tanks, specifically a sample tank for water bath heating in electrophoresis tanks. Background Technology
[0002] Electrophoresis is a technique that utilizes the property that charged particles migrate towards opposite electrodes in an electric field to separate, analyze, or purify biological macromolecules (such as nucleic acids and proteins) or small molecules. The basic process is as follows: the sample to be separated (such as DNA fragments or protein mixtures) is added to a gel that acts as a molecular sieve (commonly agarose gel or polyacrylamide gel), and then a direct current electric field is applied to both ends of the gel. Due to the differences in the charge properties, quantity, and molecular size of different components in the sample, they migrate towards their corresponding electrodes at different speeds under the combined influence of the electric field and gel resistance. After a period of time, different components will form their own bands on the gel, thus achieving separation.
[0003] Regarding patents related to electrophoresis tanks, a search revealed a Chinese utility model patent with publication number CN213172641U, which discloses an electrophoresis tank with adjustable circulating electrophoresis solution. The patent includes an electrophoresis tank body, a circulation pipe, a circulation pump, and a cover plate. The electrophoresis tank body has a placement box inside, and a fixing cylinder is welded to the inner wall of the bottom end of the electrophoresis tank body. A cavity is opened in the bottom wall of the electrophoresis tank body, and the side wall of the electrophoresis tank body is sealed and connected to the bottom flange of the circulation pipe. The bottom end of the circulation pipe penetrates the bottom wall of the electrophoresis tank body and is connected to the cavity.
[0004] Although the above-mentioned device can realize the circulation of electrophoretic solution, avoid precipitation of electrophoretic solution and ensure the electrophoretic coating effect, temperature has an important influence on the electrophoretic effect during the electrophoresis experiment. At present, the existing electrophoresis tank sample tank often suffers from uneven heating when heated, which leads to poor separation effect of electrophoretic samples and affects the accuracy and reliability of experimental results. Utility Model Content
[0005] The purpose of this invention is to provide a sample tank for water bath heating in an electrophoresis tank, so as to solve the defects mentioned in the background art.
[0006] To achieve the above objectives, a sample tank for water bath heating in an electrophoresis tank is provided, comprising a constant temperature sample tank. A stabilizing base is fixedly installed at the bottom of the constant temperature sample tank, and a temperature sensor is fixedly inserted into the side wall of the constant temperature sample tank. A heating mechanism and a dual stirring drive mechanism are installed on the lower interior side of the constant temperature sample tank. A stabilizing frame is installed on the dual stirring drive mechanism, and a drive motor is installed on the surface of the stabilizing frame. A synchronous wheel A is fixedly connected to the end of the output shaft of the drive motor, and a synchronous wheel B is installed on one side of the synchronous wheel A. The rotating shafts of both the synchronous wheel B and the synchronous wheel A are fixedly connected to the double-layer stirring mechanism through sealed bearings passing through the bottom plate of the constant temperature sample tank.
[0007] Furthermore, an isolation plate is fixedly installed inside the constant temperature sample tank, and the isolation plate covers the upper surface of the two sets of double-layer stirring mechanisms. Multiple sets of slots are equally spaced on the double-layer stirring mechanisms.
[0008] Furthermore, the heating mechanism includes a heating strip, which is laid in a meandering spiral shape at the bottom of the constant temperature sample tank. An electrical control box is installed on the outer back plate of the constant temperature sample tank, and the heating strip is electrically connected to the controller inside the electrical control box.
[0009] Furthermore, the dual stirring drive mechanism also includes a synchronous belt, one end of which is connected to synchronous pulley A, and the other end of which is connected to synchronous pulley B. At the same time, the drive motor drives the two sets of double-layer stirring mechanisms to rotate synchronously through synchronous pulley A, synchronous belt, and synchronous pulley B respectively.
[0010] Furthermore, the double-layer stirring mechanism includes a drive disk, lower blades, and upper blades. The bottom of the drive disk is fixed to the shaft of the synchronous wheel B. Three sets of lower blades are equidistantly arranged on the outer ring surface of the drive disk, and the lower blades are inclined.
[0011] Furthermore, the surface of the drive disk is provided with four sets of positioning grooves at equal intervals, and upper blades are fixedly installed inside the four sets of positioning grooves. The angle between the upper blades and the drive disk is 90°.
[0012] Compared with the prior art, the beneficial effects of this utility model are:
[0013] This design utilizes a spiraling heating strip at the bottom of the constant-temperature sample tank. Made of stainless steel, the strip is corrosion-resistant and heat-resistant, ensuring uniform heating of the water within the tank. Combined with a double-layer stirring mechanism, this promotes water flow, resulting in a more uniform temperature across the entire tank. This temperature uniformity ensures all samples are kept in the same temperature environment, guaranteeing the repeatability and comparability of experimental data and preventing errors caused by temperature variations. Attached Figure Description
[0014] Figure 1This is a three-dimensional view of the electrophoresis sample tank of this utility model;
[0015] Figure 2 This is a schematic diagram of the internal structure of the electrophoresis sample tank of this utility model;
[0016] Figure 3 This is a schematic diagram of removing the isolation sheet from the electrophoresis sample tank of this utility model.
[0017] Figure 4 This is a bottom view of the electrophoresis sample tank of this utility model.
[0018] Figure 5 This is a schematic diagram of the double-layer stirring mechanism of this utility model;
[0019] Figure 6 This is a schematic diagram of the drive structure of the double-layer stirring mechanism of this utility model;
[0020] Figure 7 This is a schematic diagram of the structural drive disk and its installation structure of this utility model.
[0021] The following are the labels in the diagram: 1. Constant temperature sample bath; 2. Heating mechanism; 21. Heating bar; 3. Stabilizer; 4. Dual stirring drive mechanism; 41. Drive motor; 42. Stabilizer frame; 43. Synchronous pulley A; 44. Synchronous belt; 45. Synchronous pulley B; 46. Double-layer stirring mechanism; 461. Drive disc; 462. Lower blade; 463. Upper blade; 5. Temperature sensor; 6. Isolation plate. Detailed Implementation
[0022] Please see Figure 1-7 This utility model provides a sample tank for water bath heating in an electrophoresis tank, including a constant temperature sample tank 1. A stabilizing base 3 is fixedly installed at the bottom of the constant temperature sample tank 1. A temperature sensor 5 is fixedly inserted into the side wall of the constant temperature sample tank 1. A heating mechanism 2 and a dual stirring drive mechanism 4 are installed on the lower inside of the constant temperature sample tank 1. A stabilizing frame 42 is installed on the dual stirring drive mechanism 4. A drive motor 41 is installed on the surface of the stabilizing frame 42. A synchronous wheel A43 is fixedly connected to the end of the output shaft of the drive motor 41. A synchronous wheel B45 is installed on one side of the synchronous wheel A43. The rotating shafts of the synchronous wheel B45 and the synchronous wheel A43 are both fixedly connected to a double-layer stirring mechanism 46 through sealed bearings passing through the bottom plate of the constant temperature sample tank 1.
[0023] Working principle: The constant temperature sample tank 1 has a sample placement area inside. A temperature sensor 5 is installed on the side wall of the constant temperature sample tank 1 to detect the temperature inside the tank. The heating strip 21 on the heating mechanism 2 is laid in a winding spiral shape at the bottom of the constant temperature sample tank 1 to uniformly heat the liquid inside. The temperature sensor 5 monitors the temperature and feeds it back to the controller. When the temperature is uneven, the controller controls the dual stirring drive mechanism 4 to drive two sets of double-layer stirring mechanisms 46 to stir, so that the water inside the constant temperature sample tank 1 flows to achieve uniform heating. The water temperature is uniform throughout the constant temperature sample tank 1.
[0024] As a preferred embodiment, an isolation plate 6 is fixedly installed inside the constant temperature sample tank 1. The isolation plate 6 covers the upper surface of the two sets of double-layer stirring mechanisms 46. Multiple sets of slots are equally spaced on the double-layer stirring mechanism 46.
[0025] The heating mechanism 2 includes a heating strip 21, which is laid in a meandering spiral shape at the bottom of the constant temperature sample tank 1. An electrical control box is installed on the outer back plate of the constant temperature sample tank 1, and the heating strip 21 is electrically connected to the controller inside the electrical control box.
[0026] like Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown: The heating strip 21 is laid in a meandering spiral shape at the bottom of the constant temperature sample tank 1. The heating strip 21 is made of stainless steel, which has the characteristics of corrosion resistance and high temperature resistance. It can uniformly heat the water inside the constant temperature sample tank 1. In conjunction with the double-layer stirring mechanism 46, the water inside the constant temperature sample tank 1 is circulated, which promotes uniform temperature in all parts of the water inside the constant temperature sample tank 1. If the sample, such as biological sample, chemical reagent, precision parts, etc., is heated unevenly in different parts of the constant temperature sample tank 1, it may lead to problems such as abnormal local reaction rate and inconsistent changes in physical properties. Temperature uniformity can ensure that all samples are in the same temperature environment, ensuring the repeatability and comparability of experimental data and avoiding experimental errors caused by temperature deviation.
[0027] To improve the reliability of results, for experiments requiring strict temperature control, such as isothermal incubation and temperature sensitivity testing, a uniform temperature environment is fundamental to ensuring the scientific validity of experimental conclusions and reducing false positives and false negatives caused by temperature fluctuations or unevenness. Uniform temperature means that the heating mechanism 2 does not need to operate continuously at high intensity due to localized low temperatures, nor does it need frequent start-stop adjustments due to localized overheating. It can maintain the target temperature with more stable power, reducing energy consumption. A uniform temperature distribution allows the temperature sensor 5 to more accurately reflect the overall true temperature, enabling the controller to efficiently adjust heating and stirring based on accurate data, reducing over-adjustment caused by localized temperature differences. "Adjustment lag" ensures rapid attainment and stabilization at the set temperature; localized high temperatures may cause samples to deform, age, or be damaged due to overheating; localized low temperatures may cause samples to freeze or solidify due to insufficient temperature; uniform temperature can avoid such damage and extend the effective service life of samples; a uniform temperature environment eliminates concerns about the unexpected effects of "local hot spots" or "cold spots," allowing operators to operate stably according to the preset procedures and reducing operational risks; the uniform water temperature inside the constant temperature sample tank 1 is a core prerequisite for ensuring experimental processing quality, improving equipment efficiency, and reducing losses and risks, especially suitable for scenarios with high temperature sensitivity and strict precision requirements.
[0028] In a preferred embodiment, the dual stirring drive mechanism 4 also includes a synchronous belt 44, one end of which is connected to the synchronous pulley A43 and the other end of which is connected to the synchronous pulley B45. At the same time, the drive motor 41 drives the two sets of double-layer stirring mechanisms 46 to rotate synchronously through the synchronous pulley A43, the synchronous belt 44, and the synchronous pulley B45.
[0029] The double-layer stirring mechanism 46 includes a drive disk 461, a lower blade 462 and an upper blade 463. The bottom of the drive disk 461 is fixed to the shaft of the synchronous wheel B45. Three sets of lower blades 462 are equidistantly arranged on the outer ring surface of the drive disk 461. The lower blades 462 are inclined.
[0030] The surface of the drive disk 461 is provided with four sets of positioning grooves at equal intervals. The upper blade 463 is fixedly installed inside each of the four sets of positioning grooves. The included angle between the upper blade 463 and the drive disk 461 is 90°.
[0031] like Figure 5 , Figure 6 and Figure 7As shown: The working mode of the dual stirring drive mechanism 4 is as follows: When the external switch of the drive motor 41 is turned on, the output shaft of the drive motor 41 drives the synchronous wheel A43 to rotate. The synchronous wheel A43 drives the synchronous wheel B45 to rotate. The synchronous wheel B45 drives the dual-layer stirring mechanism 46 to rotate. The dual-layer stirring mechanism 46 consists of a drive disk 461, lower blades 462 and upper blades 463. When the drive disk 461 rotates, the four sets of fan-shaped lower blades 462 on the outside and the four sets of upper blades 463 perpendicular to its surface on the upper side can fully stir the water in the constant temperature sample tank 1, so that the water in the tank can flow fully, thereby achieving the purpose of uniform heating and improving the separation effect of electrophoresis samples. The lower blades 462 are set at the bottom of the tank and can push the water to flow horizontally and cover the bottom of the tank. The upper blades 462 are set at the bottom of the tank and can push the water to flow horizontally and cover the bottom of the tank. The third mechanism can drive the water to tumble vertically, breaking up temperature stratification at different depths. Together, they form a horizontal and vertical three-dimensional stirring flow field, preventing temperature dead zones caused by localized stillness and quickly eliminating potential temperature differences during heating, such as higher temperatures at the bottom due to direct heating by the heating strip 21 or lag in temperature at the upper layer. Dense blades enhance the disturbance effect: the configuration of four sets of lower blades 462 and upper blades 463 creates denser water flow disturbance during rotation, causing the water to circulate within the tank, accelerating heat transfer and diffusion throughout the water, and ensuring that the temperature quickly becomes uniform throughout the tank. The double-layer stirring mechanism 46, through efficient and three-dimensional stirring, not only directly ensures the temperature uniformity of the constant-temperature sample tank 1 but also improves the practicality and reliability of the equipment in terms of energy consumption control, work efficiency, and sample processing quality. It is particularly suitable for experimental, testing, or industrial scenarios with high requirements for temperature accuracy and processing consistency.
[0032] As a preferred embodiment, an insulation layer made of polyurethane foam can be wrapped around the outside of the constant temperature sample tank 1 to improve the insulation performance of the constant temperature sample tank 1; the temperature inside the constant temperature sample tank 1 can be detected by a temperature sensor 5, which is a high-precision platinum resistance sensor that can accurately measure the temperature inside the tank.
Claims
1. A cell for electrophoresis tank water bath heating, comprising a thermostatic cell (1), characterized in that: A stabilizing seat (3) is fixedly installed at the bottom of the constant temperature sample tank (1). A temperature sensor (5) is fixedly inserted on the side wall of the constant temperature sample tank (1). A heating mechanism (2) and a dual stirring drive mechanism (4) are installed on the lower inside of the constant temperature sample tank (1). A stabilizing frame (42) is installed on the dual stirring drive mechanism (4). A drive motor (41) is installed on the surface of the stabilizing frame (42). A synchronous wheel A (43) is fixedly connected to the end of the output shaft of the drive motor (41). A synchronous wheel B (45) is installed on one side of the synchronous wheel A (43). The rotating shafts of the synchronous wheel B (45) and the synchronous wheel A (43) are both fixedly connected to a double-layer stirring mechanism (46) through a sealed bearing through the bottom plate of the constant temperature sample tank (1).
2. A sample cell bath for water bath heating of an electrophoresis cell according to claim 1, wherein: An isolation plate (6) is fixedly installed inside the constant temperature sample tank (1). The isolation plate (6) covers the upper surface of the two sets of double-layer stirring mechanisms (46). Multiple sets of slots are equally spaced on the double-layer stirring mechanism (46).
3. A sample cell bath for water bath heating of an electrophoresis cell according to claim 1, wherein: The heating mechanism (2) includes a heating strip (21), which is laid in a meandering spiral shape at the bottom of the constant temperature sample tank (1). An electrical control box is installed on the outer back plate of the constant temperature sample tank (1), and the heating strip (21) is electrically connected to the controller inside the electrical control box.
4. A sample cell bath for water bath heating of an electrophoresis cell according to claim 1, wherein: The dual stirring drive mechanism (4) also includes a synchronous belt (44), one end of which is connected to the synchronous pulley A (43), and the other end of which is connected to the synchronous pulley B (45). At the same time, the drive motor (41) drives the two sets of double-layer stirring mechanisms (46) to rotate synchronously through the synchronous pulley A (43), the synchronous belt (44), and the synchronous pulley B (45).
5. A sample cell bath for water bath heating of an electrophoresis cell according to claim 4, wherein: The double-layer stirring mechanism (46) includes a drive disk (461), a lower blade (462) and an upper blade (463). The bottom of the drive disk (461) is fixed to the shaft of the synchronous wheel B (45). Three sets of lower blades (462) are equidistantly arranged on the outer ring surface of the drive disk (461). The lower blades (462) are inclined.
6. A sample cell bath for water bath heating of an electrophoresis cell according to claim 5, wherein: The drive disk (461) has four sets of positioning grooves equidistantly arranged on its surface. Each of the four sets of positioning grooves has an upper blade (463) fixedly installed inside it. The angle between the upper blade (463) and the drive disk (461) is 90°.