Novel high performance liquid chromatograph analyzer application sample filter device

Abstract

This novel sample filtration device, used in a high-performance liquid chromatography (HPLC) analyzer, belongs to the field of auxiliary detection equipment technology. It includes a base plate, support plate, raw material tank mechanism, motor reduction mechanism, filtration mechanism, component box, valve, power module, temperature control circuit, fixing tube, and operating lever. The raw material tank mechanism, motor reduction mechanism, filtration mechanism, valve, power module, fixing tube, operating lever, base plate, and support plate are assembled together, and the temperature control circuit is installed inside the component box. The filtration mechanism includes a piston, upper cover, microporous filter plate, filter cylinder, and lead screw. The raw material tank mechanism includes an upper cylinder, movable cover, valve, and electric heating tube. This novel device can filter samples electrically or manually. Due to the electric heating and insulation mechanism, piston, and other mechanisms, and the filter plate with small-diameter micropores, it filters samples under pressure and relatively high temperature, minimizing impurities and air bubbles in the sample.

Description

Technical Field

[0001] This utility model relates to the technical field of supporting equipment for liquid chromatography analyzers, and in particular to a new type of sample filtration device for high performance liquid chromatography analyzers. Background Technology

[0002] High-performance liquid chromatography (HPLC) is an instrument that utilizes the difference in the partition ratio of a mixture between a liquid and a solid or between two immiscible liquids to separate the mixture before analysis and identification. During detection, a mobile phase (such as liquid acetonitrile-aqueous solution, acetonitrile-acetic acid aqueous solution, methanol-aqueous solution, acetonitrile-phosphoric acid aqueous solution, dichlorobenzene, N,N-dimethylformamide, isopropanol, etc.) carries the analyte solution through the outlet tube of the analyte solution chamber to the stationary phase. The components in the analyte solution are separated sequentially due to the different effects of the stationary phase. These separated components then flow sequentially with the mobile phase to a flow cell. The flow cell then performs optical detection on the different components that enter with the mobile phase, obtaining the component information of each component in the analyte solution. Currently, optical detection of solution components in flow-through cells is generally achieved using optical fibers. A flow-through cell includes the flow-through cell body, a light-reflecting flow tube inside the flow-through cell body, an optical path located outside the flow-through cell body, two optical fibers connected to the light-reflecting flow tube, an inlet pipe connected to the mobile phase tube, and an outlet pipe connected to the reservoir (the inlet and outlet pipes are connected to the inlet and outlet on the left and right sides of the light-reflecting flow tube, respectively). Specifically, after the mobile phase flows into the flow-through cell, it flows in and out through the light-reflecting flow tube. The control system of the high-performance liquid chromatography analyzer obtains the component information of the solution in the mobile phase flowing through the light-reflecting flow tube by collecting the signal changes of the light signals transmitted by the two optical fibers after passing through the light-reflecting flow tube.

[0003] In actual testing with a high performance liquid chromatography (HPLC) analyzer, the sample to be tested (the solution to be analyzed) needs to be filtered before use, mainly to remove insoluble substances and air bubbles. The specific reasons are as follows: (1) Removing insoluble substances is because insoluble substances may clog the pipeline and the micropores of the solid stationary phase, especially when the pore size is smaller, the risk of clogging is higher. (2) The presence of air bubbles in the solution to be analyzed will cause unstable flow rate and column pressure fluctuations. Removing air bubbles can ensure the stability of the flow rate of the solution to be analyzed. In the existing technology, the solution to be analyzed is usually filtered at normal pressure and temperature. Since there is no pressure in the filter and the inner diameter of the micropores of the filter plate cannot be too small, the probability of impurities in the sample to be tested after filtration is relatively high. In addition, when the temperature at the detection site is too low, the air bubbles in the sample to be tested are relatively difficult to eliminate, which will more or less have an adverse effect on the detection and analysis effect of the HPLC analyzer. Utility Model Content

[0004] To overcome the drawbacks of existing filtration devices used in high-performance liquid chromatography (HPLC) analyzers, which are limited by their structure and have the shortcomings described in the background art, this invention provides a novel sample filtration device for HPLC analyzers. This device allows operators to manually or electrically filter the sample through a filter plate with small internal pores under pressure and relatively high temperature, minimizing impurities and air bubbles in the sample and reducing their adverse effects on the normal detection and analysis of the HPLC analyzer.

[0005] The technical solution adopted by this utility model to solve its technical problem is: A novel high-performance liquid chromatography (HPLC) analyzer utilizes a sample filtration device comprising a base plate, a support plate, a raw material tank mechanism, a motor reduction mechanism, a filtration mechanism, a component box, valves, a power module, a temperature control circuit, a fixing tube, and an operating lever. The lower end of the support plate is fixedly mounted on the upper end of the base plate, the motor reduction mechanism is fixedly mounted on the upper front part of the support plate, and the upper end of the fixing tube is fixedly mounted on the lower end of the rotating shaft of the motor reduction mechanism. The filtration mechanism includes a piston, a top cover, a microporous filter plate, a filter cylinder, and a lead screw. An outlet pipe is fixedly mounted on the lower end of the filter cylinder, and an inlet pipe is fixedly mounted on one side of the upper end of the filter cylinder. A lead screw seat is fixedly mounted on the upper end of the top cover, and the top cover is threadedly fixed to the outer side of the upper end of the filter cylinder. The lead screw and lead seat are threaded together. The lower part of the lead screw is rotatably mounted inside the upper part of the piston, and the piston is located inside the filter cylinder. The lower end of the filter cylinder has a support ring, and the microporous filter plate is set on the support ring. The raw material box mechanism includes a cylinder, a movable cover, and an electric heating tube. The outer end of the cylinder has a heating chamber, and the electric heating tube is fixedly installed inside the heating chamber. The movable cover and the upper end of the cylinder are attracted together by a magnet. The lower end of the cylinder is fixedly installed with an outlet pipe A. The lower end of the outlet pipe A is fixedly installed with a valve, and the other end of the valve is fixedly installed with the side end of the inlet pipe. The temperature control circuit is installed in the component box. The power output terminal of the temperature control circuit is electrically connected to the power input terminal of the electric heating tube.

[0006] Furthermore, a sealing ring is fixedly installed on the outer end of the microporous filter plate, and the sealing ring is in sealing contact with the inner wall of the filter cylinder.

[0007] Furthermore, the upper end of the lead screw has an operating hole, and the operating rod is laterally movably sleeved within the operating hole.

[0008] Furthermore, the lower ends of the fixed tube have threaded holes and openings respectively. Without the operating rod installed, a fixing bolt passes through the opening of the fixed tube and the operating hole of the threaded rod, and is screwed into the threaded hole of the fixed tube.

[0009] Furthermore, a one-way valve is fixedly installed on the upper end of the movable cover.

[0010] Furthermore, the temperature control circuit includes a relay and a temperature control switch that are electrically connected. The temperature control switch is fixedly installed outside the lower end of the heating chamber, and one end of the temperature control switch is connected to the positive power input terminal of the relay.

[0011] Furthermore, the temperature control switch is a normally closed contact temperature switch.

[0012] Compared with the prior art, the advantages of this utility model are as follows: Under the action of relevant mechanisms, based on the installation of a motor reduction mechanism, the operator can subsequently filter the sample to be tested electrically, or without installing a motor, filter the sample to be tested manually (for example, in the case of motor failure in the electric application; in specific production, the manufacturer can also sell the complete equipment for filtering the sample to be tested manually or electrically according to customer needs); due to the presence of an electric heating and heat preservation mechanism and piston mechanism, and a filter plate with a small inner diameter micropore, the sample to be tested is filtered under pressure and relatively high temperature, minimizing the adverse effects of impurities and air bubbles in the sample on the normal detection and analysis of the high performance liquid chromatography analyzer. Attached Figure Description

[0013] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0014] Figure 1 This is a structural diagram of the present invention with the motor reduction mechanism installed.

[0015] Figure 2 This is a structural diagram of the present invention without the motor reduction mechanism installed.

[0016] Figure 3 This is the circuit diagram of this utility model. Detailed Implementation

[0017] Figure 1 , 2As shown in Figure 3, the sample filtration device used in the novel high-performance liquid chromatography analyzer includes a base plate 1, a support plate 2, a raw material box mechanism 3, a motor reduction mechanism M, a filtration mechanism 4, a power switch S, a component box 5, a valve 6, a power module W1, a temperature control circuit 8, a fixing tube 7, and an operating lever 10. The lower end of the support plate 2 is fixedly installed in the middle of the upper rear end of the base plate 1. The middle of the upper front end of the support plate 2 has four fixing screw holes 21. The motor reduction mechanism M is screwed into the internal threads of the four screw holes 211 by four bolts and fixedly installed in the middle of the upper front end of the support plate 2. The fixing tube 7 is vertically distributed and fixedly installed at the lower end of the rotating shaft of the motor reduction mechanism M. The filtration mechanism includes a piston 41. The filter consists of a top cover 42, a microporous filter plate 43, a filter cylinder 44, and a lead screw 45. The filter cylinder 44 has an open upper end and a closed lower end. A liquid outlet pipe 441, communicating with the interior, is fixedly installed in the middle of the lower outer end of the filter cylinder 44. A liquid inlet pipe 442, communicating with the interior, is fixedly installed in the middle of the upper left outer end of the filter cylinder. The upper outer end of the filter cylinder 44 has external threads, and the lower inner end of the top cover 42 has internal threads. A lead screw seat 421, communicating with the lower end of the top cover 42, is fixedly installed in the middle of the upper end of the top cover 42. A bearing seat 411 is installed in the middle of the upper end of the piston 41. The top cover 42 is threadedly fixed to the upper outer end of the filter cylinder 44. The lead screw 45 and the lead screw seat 421 are threadedly installed together. The lower part of the lead screw 45 is located at the lower end of the upper cover 42, and the lower part of the lead screw 45 is fixedly installed in the inner ring of the bearing seat 411, with the piston located inside the filter cylinder 44; the lower end of the filter cylinder 44 has a hollow annular support ring 443, and the microporous filter plate 43 is set on the support ring 443; the raw material box mechanism 3 includes a cylinder 31 with an open upper end and a closed lower end, a movable cover 32, and electric heating tubes RT. The outer end of the cylinder 31 has a double-layer hollow structure as a heating chamber 311, and six electric heating tubes RT are distributed in a ring and fixedly installed in the heating chamber 311. The wires connected to the six electric heating tubes RT are connected in parallel and led out downward from the opening at the lower end of the heating chamber 311. The opening is sealed with heat-resistant sealant; a hollow annular lower magnet ring 34 is glued and fixedly installed on the outer side of the upper end of the cylinder 31, and a hollow annular upper magnet ring 35 is glued and fixedly sealed on the outer side of the lower end of the movable cover 32. The movable cover 32 and the upper end of the cylinder 31 are attracted together by magnets; a liquid outlet pipe A312 that communicates with its interior is fixedly installed on the outer side of the middle of the lower end of the cylinder 31. The lower end of the liquid outlet pipe A312 is fixedly installed together with one end of the valve 6, and the other end of the valve 6 is fixedly installed together with the side end of the liquid inlet pipe 442. The power module W1, the power switch S and the temperature control circuit 8 are installed on the circuit board inside the component box 5. The component box 5 is fixedly installed on the front right end of the base plate 1.The power input terminals 1 and 2 of the power module W1 and the two control power input terminals of the relay K in the temperature control circuit are connected to the two poles of the 220V AC power supply via wires. The power output terminals 3 and 4 of the power module W1 and the other end of the temperature control switch T and the negative power input terminal of the relay K in the temperature control circuit are connected to the two normally open contacts of the relay K in the temperature control circuit and the two ends of the power input of the six electric heating tubes RT via wires. The power input terminal 1 of the power switch S is connected to one pole of the 220V AC power supply via wires. The power output terminals 2 and 3 of the power switch S are connected to the two ends of the running capacitor C of the motor reduction mechanism M via wires. The main power input terminal of the motor reduction mechanism M is connected to the other pole of the 220V AC power supply via wires.

[0018] Figure 1 , 2 As shown in Figure 3, the lower side of the upper magnet ring 35 and the upper side of the lower magnet ring 34 have opposite polarities. A plastic sealing ring 431 is fixedly installed on the outer end of the annular microporous filter plate, and the sealing ring 431 is in sealing contact with the inner wall of the filter cylinder 44. There is an operating hole 451 in the middle of the upper end of the lead screw 45, and the operating rod 10 is movably sleeved in the operating hole 451. There is a threaded hole and an opening at the lower part of the left and right ends of the fixed tube 7, respectively. Without the operating rod installed, a fixing bolt 9 passes through the opening at the right end of the fixed tube and the lead screw operating hole 451, and is screwed into the threaded hole at the left end of the fixed tube. A one-way valve 36 is fixedly installed in the middle of the upper end of the movable cover, and the exhaust port of the one-way valve 36 is located under the movable cover 32 (external air can enter the cylinder 31 through the one-way valve 36 to prevent negative pressure from being generated inside). The temperature control circuit includes a relay K and a temperature control switch T connected via circuit board wiring. The temperature control switch T is fixedly installed on the lower end of the heating chamber, with its sensing surface in close contact with the lower outer end of the heating chamber (where the cylinder is a single-layer structure). One end of the temperature control switch T is connected to the positive power input terminal of the relay K. The temperature control switch T is a KSD301 normally closed contact temperature switch (30℃). The motor reduction mechanism M is a coaxial motor gear reducer with a working voltage of 220V DC and a power of 1.5KW. Its motor is a capacitor-run motor (capacitor C is 12UF / 400V). The motor heat pipe RT is a 500W stainless steel armored straight electric heating tube. The microporous filter plate 43 has a pore size of 40μm. The power module W1 is an AC 220V to DC 12V power module. The relay K is DC12V. The power switch S handle is located outside the opening at the front of the component box 5.

[0019] Figure 1 , 2As shown in Figure 3, after the 220V AC power enters the power input terminal of the power module W1, the power module W1 outputs a stable 12V DC power supply which enters the power input terminal of the temperature control circuit. In this novel manual method for filtering the sample to be tested (without the motor reduction mechanism and fixing tube 7 installed, the operating rod 10 is fitted into the upper operating hole 451 of the lead screw 45, and the outlet pipe 441 at the lower end of the filter cylinder 45 is connected to the inlet pipe of the analytical solution chamber of the high-performance liquid chromatograph via a flexible hose; the height of the analytical solution inlet pipe is lower than the height of the outlet pipe 441), the movable cover 32 is removed, and then the sample to be filtered is poured into the cylinder 31, and the movable cover 32 is closed. Then, the valve 6 is opened, and the sample enters the filter cylinder 44 through the outlet pipe A312. Then, the operator rotates the lead screw 45 clockwise... The rod moves downward along the internal thread of the lead screw 421, and the lower end of the lead screw 421 pushes the piston 41 vertically downward (the lower end of the lead screw rotates along the inner ring of the bearing in the bearing housing, and the piston does not rotate, but only moves vertically downward or upward). The piston 41 compresses the sample to be tested in the filter cylinder 44. The compressed sample to be tested is filtered through the microporous filter plate 43 and flows out through the liquid outlet pipe 441 into the solution chamber to be analyzed. After filtration, the operator operates the operating rod 451 counterclockwise, and the lead screw moves upward along the internal thread of the lead screw 421. The lower end of the lead screw 45 drives the piston 41 vertically upward. The operation stops when the lower end of the piston 41 moves onto the liquid outlet pipe A312.When filtering the sample using this novel electric method, (remove the operating rod 451, connect the fixed tube 7 and the lead screw with threads, pass the fixing bolt 9 through the operating hole 451 on the right end of the fixed tube and screw it into the threaded hole on the left end of the fixed tube, then fix the motor reduction mechanism M to the upper front end of the support plate 2, connect the outlet pipe 441 at the lower end of the filter cylinder 45 to the inlet pipe of the analytical solution chamber of the high performance liquid chromatograph via a hose, remove the movable cover 32, then pour the sample to be filtered into the cylinder 31 and close the movable cover 32, then open the valve 6, the compressed sample enters the filter cylinder 44 through the outlet pipe A312, the operator moves the power switch S to the left (pins 1 and 2 of the power switch S are connected), one pole of the 220V AC power supply enters one end of the motor running capacitor C of the motor reduction mechanism M through pins 1 and 2 of the power switch S, and the other pole of the 220V AC power supply enters the main power input terminal of the motor, the shaft of the motor reduction mechanism M drives) The fixed tube 7 rotates clockwise, which in turn rotates the lead screw 45. The lead screw moves downward along the internal thread of the lead screw seat 421, and the lower end of the lead screw 45 pushes the piston 41 downward vertically. The piston 41 compresses the sample to be tested in the filter cylinder 44. The compressed sample is filtered through the microporous filter plate 43 and then flows out through the outlet pipe 442 into the analytical solution chamber. After filtration, the operator turns the power switch S to the right (pins 1 and 3 of the power switch S are connected). One pole of the 220V AC power supply enters the other end of the motor running capacitor C of the motor reduction mechanism M through pins 1 and 3 of the power switch S. The other pole of the 220V AC power supply enters the main power input terminal of the motor. The shaft of the motor reduction mechanism M drives the fixed tube 7 to rotate counterclockwise, which in turn rotates the lead screw 45 counterclockwise. The lead screw moves upward along the internal thread of the lead screw seat 421, and the lower end of the lead screw 421 drives the piston 41 upward vertically. The operation stops when the lower end of the piston 41 reaches the outlet pipe A312. After filtering all the samples to be tested, the valve 6 is closed.

[0020] Figure 1 , 2 As shown in Figure 3, before the actual operation of this new device, and before valve 6 is opened, when the temperature inside the cylinder 31 is below a certain level (e.g., below 30°C), the internal contacts of the temperature control switch T close. In this way, 12V power is supplied to the positive power input terminal of relay K via the temperature control switch T. Relay K is energized and closes the power input terminal and the normally open contact terminal. Thus, the six electric heating tubes RT are simultaneously energized and heat the solution to be analyzed inside the cylinder 31. When the temperature inside the cylinder 31 exceeds 30°C, the internal contacts of the temperature control switch T open, and the six electric heating tubes RT cease to energize and heat the solution to be analyzed inside the cylinder 31. This process is continuously repeated, and this new device can maintain the temperature of the solution to be analyzed inside the cylinder at approximately 30°C.

[0021] Figure 1 , 2As shown in Figure 3, through the above-described process, this new invention, under the action of relevant mechanisms, allows for electric filtering of the sample under test by the operator, based on the installation of a motor reduction mechanism. Alternatively, it can filter the sample manually without the motor (for example, in the case of motor failure during electric application). In actual production, the manufacturer can also sell the complete equipment for filtering the sample manually or electrically, according to customer needs. Due to the presence of an electric heating and insulation mechanism and a piston mechanism, the filter plate with a small inner diameter micropore filters the sample under pressure and relatively high temperature, minimizing the adverse effects of impurities and air bubbles in the sample on the normal detection and analysis of the high-performance liquid chromatography analyzer.

[0022] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. It will be apparent to those skilled in the art that this utility model is limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or basic characteristics of this utility model. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this utility model is defined by the appended claims rather than the foregoing description, and thus all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this utility model.

[0023] Furthermore, it should be understood that although this specification describes the embodiments, the embodiments do not necessarily contain only one independent technical solution. This way of describing the specification is only for clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in the embodiments can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A sample filtration device for a novel high-performance liquid chromatography analyzer, comprising a base plate, a support plate, a raw material tank mechanism, a motor reduction mechanism, a filtration mechanism, a component box, valves, a power module, a temperature control circuit, a fixing tube, and an operating lever; characterized in that, The lower end of the support plate is fixedly installed on the upper end of the base plate, the motor reduction mechanism is fixedly installed on the upper front part of the support plate, and the upper end of the fixing tube is fixedly installed on the lower end of the rotating shaft of the motor reduction mechanism; the filtration mechanism includes a piston, a top cover, a microporous filter plate, a filter cylinder, and a lead screw. The lower end of the filter cylinder is fixedly installed with an outlet pipe, and the upper side of the filter cylinder is fixedly installed with an inlet pipe. The upper end of the top cover is fixedly installed with a lead screw seat. The top cover is fixedly installed on the upper outside of the filter cylinder by threads. The lead screw and the lead screw seat are installed together by threads. The lower part of the lead screw is rotatably installed inside the upper part of the piston, and the piston is located inside the filter cylinder. The filter cylinder has a support ring at its lower end, and the microporous filter plate is mounted on the support ring. The raw material box mechanism includes a cylinder, a movable cover, and an electric heating tube. The outer end of the cylinder has a heating chamber, and the electric heating tube is fixedly installed inside the heating chamber. The movable cover and the upper end of the cylinder are magnetically attracted together. An outlet pipe A is fixedly installed at the lower end of the cylinder. The lower end of the outlet pipe A is fixedly installed with a valve, and the other end of the valve is fixedly installed with the side end of the inlet pipe. The temperature control circuit is installed in the component box. The power output terminal of the temperature control circuit is electrically connected to the power input terminal of the electric heating tube.

2. The novel high-performance liquid chromatography analyzer applied to the sample filtering device to be measured according to claim 1, characterized in that, A sealing ring is fixedly installed on the outer end of the microporous filter plate, and the sealing ring is in sealing contact with the inner wall of the filter cylinder.

3. The novel high performance liquid chromatography analyzer applied to the sample filtering device to be measured according to claim 1, characterized in that, The upper end of the lead screw has an operating hole, and the operating rod moves laterally within the operating hole.

4. The new type of high performance liquid chromatography analyzer applied to the sample filtering device to be measured according to claim 1, characterized in that, The lower ends of the fixed tube have threaded holes and openings respectively. Without the operating rod installed, a fixing bolt passes through the opening of the fixed tube and the operating hole of the threaded rod, and is screwed into the threaded hole of the fixed tube.

5. The sample filtration device for the novel high-performance liquid chromatography analyzer according to claim 1, characterized in that, A one-way valve is fixedly installed on the upper part of the movable cover.

6. The novel high performance liquid chromatography analyzer applied to the sample filtering device to be measured according to claim 1, characterized in that, The temperature control circuit includes an electrically connected relay and a temperature control switch. The temperature control switch is fixedly installed on the outside of the lower end of the heating chamber, and one end of the temperature control switch is connected to the positive power input terminal of the relay.

7. The novel high performance liquid chromatography analyzer applied to the sample filtering device to be measured according to claim 1, characterized in that, The temperature control switch is a normally closed contact temperature switch.

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