A bioreactor device for tissue culture plantlet cultivation

Abstract

The utility model discloses a kind of bioreactor devices for tissue culture seedling culture, belong to plant tissue culture field, comprising: reactor main body, transparent cover, culture plate, air pressure pump and liquid supplementing device, culture plate is fixedly installed in reactor main body, culture plate is sealingly connected with reactor main body inner wall, drain pipe and multiple culture tanks are provided on culture plate, drain pipe extends to the bottom wall of reactor main body;Transparent cover is sealingly connected at the top of reactor main body by multiple bolts, and air pipe is provided on transparent cover;Air inlet pipe is provided on the lateral wall of reactor main body, and air inlet pipe is located below culture plate, and air pressure pump is communicated with air inlet pipe;Liquid supplementing device is communicated with reactor main body.The utility model pressurizes culture solution in reactor main body to culture plate by air pressure pump, and culture solution is backflowed to reactor main body under the action of gravity, intermittent immersion culture is realized, and the culture quality of seedling and somatic embryo is improved.

Description

Technical Field

[0001] This utility model belongs to the field of plant tissue culture, and in particular relates to a bioreactor device for tissue culture seedling cultivation. Background Technology

[0002] Plant tissue culture technology is widely used in crop breeding, flower, fruit and vegetable, and forestry seedling propagation. Currently, the most mainstream plant tissue culture method at home and abroad is tissue culture bottle / agar semi-solid culture. However, existing plant tissue culture technology has limitations. For example, explants are prone to the accumulation of browning substances during culture, leading to the interruption of tissue and new shoot development. Using agar or gelatin as a semi-solid support affects the rapid transfer of nutrients, which can only be absorbed through contact. Different developmental stages of tissue culture seedlings require different hormone and nutrient ratios, which can only be achieved by transferring them to different culture media. Therefore, there is an urgent need for those skilled in the art to propose a bioreactor device for tissue culture seedling cultivation to solve the above problems. Utility Model Content

[0003] In view of this, the present invention provides a bioreactor device for tissue culture seedling cultivation to solve the above problems.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] A bioreactor device for tissue culture seedling cultivation includes: a reactor body, a transparent cover, a culture plate, a pneumatic pump, and a liquid replenishment device. The culture plate is fixedly installed in the reactor body and is sealed to the inner wall of the reactor body. The culture plate is provided with a drain pipe and multiple culture tanks, and the drain pipe extends towards the bottom wall of the reactor body. The transparent cover is sealed to the top of the reactor body by multiple bolts and is provided with a vent pipe. An air inlet pipe is provided on the side wall of the reactor body, located below the culture plate, and the pneumatic pump is connected to the air inlet pipe. The liquid replenishment device is connected to the reactor body.

[0006] Furthermore, it also includes a three-way valve, a first check valve, and a second check valve. One end of the three-way valve is connected to the air inlet pipe, and the other two ends are connected to the air pressure pump and the liquid replenishment device, respectively. The first check valve is installed on the connecting pipe between the three-way valve and the air pressure pump. The second check valve is installed on the connecting pipe between the three-way valve and the liquid replenishment device.

[0007] Furthermore, it also includes a first air filter and a second air filter, wherein the first air filter is installed at the outlet end of the vent pipe; and the second air filter is installed on the connecting pipe between the first one-way valve and the air pressure pump.

[0008] Furthermore, the fluid replenishment device includes a peristaltic pump, a storage bottle, and a suction tube. The suction tube is fixed in the cap of the storage bottle, with one end extending into the storage bottle and the other end connected to the peristaltic pump. The peristaltic pump is connected to the second one-way valve.

[0009] Furthermore, the replenishment device also includes a third air filter, which is installed on the cap of the liquid storage bottle to allow communication between the inside and outside of the liquid storage bottle and to prevent bacteria from entering the liquid storage bottle.

[0010] Furthermore, a top cover is provided at the center of the top of the transparent cover.

[0011] The beneficial effects of this utility model are as follows:

[0012] This invention uses a pneumatic pump to pressurize the culture medium inside the reactor body onto the culture plate, and then, under gravity, returns it to the reactor body through a drain pipe. This achieves intermittent immersion culture of seeds, seedlings, or somatic embryos on the culture plate, reducing vitrification and improving survival rate. Multiple seedlings can be cultured simultaneously within the reactor body, increasing culture efficiency. A three-way valve connects both the pneumatic pump and the liquid replenishment device, and a first and second one-way valve ensure both gas and liquid supply, enabling culture of the entire life cycle or longer periods without affecting tissue development or plant growth, and avoiding multiple transfers. A first and second air filter ensure that filtered, sterile air enters and exits the reactor body and the transparent enclosure. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0014] Figure 1 This is a schematic diagram of a bioreactor device used for tissue culture seedling cultivation.

[0015] Figure 2 It is a three-dimensional view of the reactor body and the transparent cover.

[0016] In the figure:

[0017] 10-Reactor body, 11-Air inlet pipe, 20-Transparent cover, 21-Ventilation pipe, 22-Top cover, 30-Cultivation plate, 31-Drain pipe, 40-Air pressure pump, 51-Peristaltic pump, 52-Storage bottle, 53-Suction pipe, 54-Third air filter, 60-Three-way valve, 70-First check valve, 80-Second check valve, 90-First air filter, 100-Second air filter. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] See attached document Figure 1-2 As shown, this utility model provides a bioreactor device for tissue culture seedling cultivation, including: a reactor body 10, a transparent cover 20, a culture plate 30, a pneumatic pump 40, and a liquid replenishment device. The culture plate 30 is fixedly installed in the reactor body 10 and is sealed to the inner wall of the reactor body 10. The culture plate 30 is provided with a drain pipe 31 and multiple culture tanks, and the drain pipe 31 extends to the bottom wall of the reactor body 10. The transparent cover 20 is sealed to the top of the reactor body 10 by multiple bolts, and a vent pipe 21 is provided on the transparent cover 20. An air inlet pipe 11 is provided on the side wall of the reactor body 10, and the air inlet pipe 11 is located below the culture plate 30. The pneumatic pump 40 is connected to the air inlet pipe 11. The liquid replenishment device is connected to the reactor body 10. The culture medium inside the reactor body 10 is pumped onto the culture plate 30 by the pneumatic pump 40, immersing the seeds, seedlings, or somatic embryos. Then, under the action of gravity, the culture medium returns to the reactor body 10 through the drain pipe 31, at which point the seeds, seedlings, or somatic embryos are no longer immersed in the culture medium. The pneumatic pump 40 repeatedly pumps the culture medium onto the culture plate 30, realizing intermittent immersion culture of seedlings or somatic embryos on the culture plate 30, reducing vitrification and improving the survival rate. Multiple seedlings can be cultured simultaneously in the reactor body 10, improving the culture efficiency.

[0020] In a preferred embodiment, a bioreactor device for tissue culture seedling cultivation further includes a three-way valve 60, a first one-way valve 70, and a second one-way valve 80. One end of the three-way valve 60 is connected to the air inlet pipe 11, and the other two ends are connected to the air pressure pump 40 and the replenishment device, respectively. The first one-way valve 70 is located on the connecting pipe between the three-way valve 60 and the air pressure pump 40. The second one-way valve 80 is located on the connecting pipe between the three-way valve 60 and the replenishment device. When it is necessary to press the culture medium onto the culture plate 30, the replenishment device does not work. The gas output by the air pressure pump 40 enters the space between the culture plate 30 and the reactor body 10 through the first one-way valve 70, the three-way valve 60, and the air inlet pipe 11, pressing the culture medium in the reactor body 10 upward onto the culture plate 30 through the drain pipe 31 to submerge the seeds, seedlings, or somatic embryos. During this process, due to the action of the second one-way valve 80, the gas from the air pressure pump 40 will not enter the replenishment device.

[0021] In a preferred embodiment, a bioreactor device for tissue culture seedling cultivation further includes a first air filter 90 and a second air filter 100. The first air filter 90 is installed at the outlet end of the vent pipe 21; the second air filter 100 is installed on the connecting pipe between the first one-way valve 70 and the air pressure pump 40. The first air filter 90 and the second air filter 100 ensure that filtered sterile air enters and exits the reactor body 10 and the transparent cover 20.

[0022] The replenishment device includes a peristaltic pump 51, a storage bottle 52, and a suction tube 53. The suction tube 53 is fixed in the cap of the storage bottle 52, with one end extending into the storage bottle 52 and the other end connected to the peristaltic pump 51. The peristaltic pump 51 is connected to the second one-way valve 80. When it is necessary to replenish the culture medium into the reactor body 10, the pneumatic pump 40 does not work, and the peristaltic pump 51 replenishes the culture medium in the storage bottle 52 into the reactor body 10 through the suction tube 53 and the air inlet pipe 11.

[0023] The replenishment device also includes a third air filter 54, which is installed on the cap of the liquid storage bottle 52 for communication between the inside and outside of the liquid storage bottle 52 and to prevent bacteria from entering the liquid storage bottle 52.

[0024] In one optional embodiment, a top cover 22 is provided at the center of the top of the transparent cover 20 to facilitate the removal of the transparent cover 20.

[0025] Working principle:

[0026] The gas output from the pneumatic pump 40 enters the space between the culture plate 30 and the reactor body 10 through the first one-way valve 70, the three-way valve 60, and the air inlet pipe 11. This pushes the culture medium in the reactor body 10 upwards onto the culture plate 30 through the drain pipe 31, immersing the seeds, seedlings, or somatic embryos. During this process, due to the action of the second one-way valve 80, the gas from the pneumatic pump 40 does not enter the replenishment device. Then, under the action of gravity, the gas returns to the reactor body 10 through the drain pipe 31. At this time, the seeds, seedlings, or somatic embryos are no longer immersed in the culture medium. The pneumatic pump 40 repeatedly pushes the culture medium onto the culture plate 30, achieving intermittent immersion culture of the seedlings or somatic embryos on the culture plate 30, reducing vitrification and improving the survival rate. When replenishment is needed, the pneumatic pump 40 stops working, and the peristaltic pump 51 replenishes the culture medium in the storage bottle 52 into the reactor body 10 through the suction pipe 53 and the air inlet pipe 11.

[0027] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0028] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A bioreactor device for tissue culture, characterized by, include: The reactor body (10), transparent cover (20), culture plate (30), air pressure pump (40), and liquid replenishment device are provided. The culture plate (30) is fixedly installed in the reactor body (10) and sealed to the inner wall of the reactor body (10). The culture plate (30) is provided with a drain pipe (31) and multiple culture tanks. The drain pipe (31) extends to the bottom wall of the reactor body (10). The transparent cover (20) is sealed to the top of the reactor body (10) by multiple bolts. The transparent cover (20) is provided with a vent pipe (21). An air inlet pipe (11) is provided on the side wall of the reactor body (10). The air inlet pipe (11) is located below the culture plate (30). The air pressure pump (40) is connected to the air inlet pipe (11). The liquid replenishment device is connected to the reactor body (10).

2. A bioreactor apparatus for tissue culture plantlet cultivation according to claim 1, wherein, It also includes a three-way valve (60), a first check valve (70), and a second check valve (80). One end of the three-way valve (60) is connected to the air inlet pipe (11), and the other two ends are connected to the air pump (40) and the liquid replenishment device, respectively. The first check valve (70) is located on the connecting pipeline between the three-way valve (60) and the air pump (40). The second check valve (80) is located on the connecting pipeline between the three-way valve (60) and the liquid replenishment device.

3. A bioreactor apparatus for tissue culture plantlet cultivation according to claim 2, wherein, It also includes a first air filter (90) and a second air filter (100), the first air filter (90) being installed at the outlet end of the vent pipe (21); the second air filter (100) being installed on the connecting pipe between the first one-way valve (70) and the air pump (40).

4. A bioreactor device for tissue culture seedling cultivation according to claim 2, characterized in that, The fluid replenishment device includes a peristaltic pump (51), a storage bottle (52), and a suction tube (53). The suction tube (53) is fixed in the cap of the storage bottle (52), with one end extending into the storage bottle (52) and the other end connected to the peristaltic pump (51). The peristaltic pump (51) is connected to the second one-way valve (80).

5. A bioreactor apparatus for tissue culture plantlet cultivation according to claim 4, wherein The replenishment device also includes a third air filter (54), which is installed on the cap of the liquid storage bottle (52) for communicating between the inside and outside of the liquid storage bottle (52) and preventing bacteria from entering the liquid storage bottle (52).

6. The bioreactor apparatus for tissue culture according to claim 1, wherein, The transparent cover (20) has a top cover (22) at the center of its top.

Images