Multistage filter device for stem cell cultivation

Abstract

The utility model discloses a stem cell cultivation multistage filter device, including device casing, and be located device casing surface's block cover, double -deck movable filter screen subassembly and movable adjusting structure, the utility model discloses the synergic design of double -deck movable filter screen subassembly and movable adjusting structure has realized the dynamic self -adaptation switching when filter screen block. When the upper filter screen is blocked because of the accumulation of cell fragments, the operator only needs to pull up the adjusting rod, and the pulling structure can drive the filter screen framework to make the upper filter screen whole turn over and closely adhere with the bottom filter screen, forcing the accumulated cell liquid to be directly introduced into the bottom filter screen for secondary filtration under the action of gravity, without disassembling or replacing the filter screen. In the normal filtering stage, the lower adjusting rod can make the upper filter screen form a conical structure, and form a uniform gap with the bottom filter screen, ensuring the stratified filtration of the cell liquid while reducing the risk of local blockage.

Description

Technical Field

[0001] This utility model relates to the field of biological experiments, specifically a multi-stage filtration device for stem cell culture. Background Technology

[0002] In stem cell culture, multi-stage filtration devices are crucial tools for ensuring cell purity and culture quality, playing a key role, especially in primary cell isolation and conditioned medium preparation. During primary cell isolation, tissue debris and non-target cells are removed step-by-step through filters of different pore sizes to enrich highly viable stem cells. Similarly, in conditioned medium preparation, multi-stage filtration removes residual cells and impurities to obtain a high-purity culture medium. Currently, common multi-stage filtration devices mainly fall into two categories: centrifugally driven and fixed-filter series-connected. The former relies on centrifugal force to accelerate liquid penetration through the filter, but this poses a potential risk of cell viability. The latter uses detachable filter assemblies for graded screening, but frequent disassembly during operation introduces a risk of contamination.

[0003] The core drawback of existing technology is that cell debris and proteins in high-viscosity samples tend to accumulate on the filter surface, causing pore blockage. This forces the process to be interrupted and requires frequent cleaning, maintenance, or filter replacement. This not only prolongs the preparation cycle and increases costs, but may also lead to a decrease in cell activity due to repeated operations, thus restricting the standardization and large-scale development of stem cell culture. Utility Model Content

[0004] To solve the above problems, this utility model provides the following technical solution:

[0005] A multi-stage filtration device for stem cell culture, comprising a device housing and a sealing cap disposed on the surface of the device housing, characterized in that it further comprises:

[0006] A double-layer movable filter assembly includes a fixed sleeve disposed inside the device housing, a bottom filter disposed inside the fixed sleeve, an upper filter disposed on the surface of the bottom filter, and a filter skeleton disposed on the upper filter.

[0007] The adjustable structure includes an adjusting rod disposed on the surface of the sealing cover and a pulling structure disposed on the surface of the filter screen frame. One end of the adjusting rod extends into the interior of the device housing and is connected to the bottom filter screen and the upper filter screen. The pulling structure can connect the filter screen frame to the adjusting rod and the fixing sleeve.

[0008] When the adjusting rod is pressed down, the upper filter screen can form a cone-shaped structure under the action of the traction structure. At this time, a gap is left between the upper filter screen and the bottom filter screen to allow cell fluid to flow in.

[0009] When the adjusting rod is pulled up, the upper filter screen can fit into the bottom filter screen.

[0010] Based on the above technical solution, the present invention can be further improved as follows.

[0011] Furthermore, a fixing ring is provided at one end of the adjusting rod that extends into the device housing. Both the bottom filter and the upper filter are connected to the fixing ring, and a filter is provided inside the fixing ring.

[0012] Furthermore, the filter mesh frame is a rod-shaped structure made of elastic material.

[0013] Furthermore, the tensioning structure includes an elastic rope connecting the end of the filter frame and a connecting rope connecting the end of the filter frame to the inside of the fixing sleeve.

[0014] Furthermore, a connecting structure is provided between the fixing ring and the adjusting rod, and an opening is provided between the connecting structure and the fixing ring to allow cell fluid to pass through.

[0015] Furthermore, a limiting rope is provided between the fixing ring and the fixing sleeve, and the limiting rope is connected to the bottom filter screen.

[0016] Furthermore, the adjusting rod has an inlet channel inside, an outlet on the surface of the adjusting rod inside the device housing, and an inlet at the end of the adjusting rod extending to the outside of the device housing.

[0017] Furthermore, the liquid inlet of the adjusting rod is provided with a liquid inlet hopper, and the opening of the liquid inlet hopper is provided with a sealing cap.

[0018] Furthermore, the sealing cover is provided with an operating handle on its surface.

[0019] Furthermore, the device housing is provided with a bottom filter structure, which includes a mesh frame and a secondary filter screen disposed inside the mesh frame.

[0020] Beneficial effects

[0021] Compared with existing technologies, this invention achieves dynamic adaptive switching when the filter becomes clogged through the coordinated design of a double-layer movable filter assembly and a movable adjustment structure. When the upper filter becomes clogged due to the accumulation of cell debris, the operator only needs to pull up the adjustment rod. The pulling structure will cause the filter frame to flip up the entire upper filter and fit tightly against the bottom filter, forcing the accumulated cell fluid to be directly introduced into the bottom filter for secondary filtration under gravity, without the need to disassemble or replace the filter. During normal filtration, pressing down the adjustment rod will cause the upper filter to form a cone-shaped structure, creating a uniform gap between it and the bottom filter, ensuring stratified filtration of the cell fluid while reducing the risk of local clogging. This design significantly reduces the frequency of manual intervention, avoids contamination introduced by open operation, and extends the continuous working time of the filter, making it particularly suitable for the large-scale processing of high-viscosity stem cell suspensions. Attached Figure Description

[0022] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a cross-sectional view of the utility model when the adjusting rod is not pulled up.

[0024] Figure 2 A cross-sectional view of the utility model when the adjusting rod is pulled up;

[0025] Figure 3 This is a schematic diagram of the top section structure of the utility model;

[0026] Figure 4 A three-dimensional structural diagram of the double-layer filter structure of the utility model;

[0027] Figure 5 A three-dimensional structural diagram of the adjusting rod and connecting structure of the utility model;

[0028] Figure 6 For utility model Figure 1 Enlarged structural diagram at point A in the middle;

[0029] Figure 7 For utility model Figure 1 Enlarged structural diagram at point B;

[0030] Figure 8 For utility model Figure 1 Enlarged structural diagram at point C;

[0031] The attached diagram lists the components represented by each number as follows:

[0032] 1. Device housing; 2. Sealing cover; 3. Double-layer movable filter assembly; 31. Fixed sleeve; 32. Bottom filter; 33. Upper filter; 34. Filter frame; 4. Movable adjustment structure; 41. Adjusting rod; 42. Pulling structure; 421. Elastic rope; 422. Connecting rope; 423. Limiting rope; 43. Connecting structure; 5. Liquid inlet hopper; 6. Sealing cover; 7. Bottom filter structure. Detailed Implementation

[0033] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. Several embodiments of this utility model are shown in the drawings. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that the disclosure of this utility model will be more thorough and complete.

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0035] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0036] It should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the technical product is in use. They are only for the convenience of describing the technology and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the technology.

[0037] Furthermore, terms such as "horizontal," "vertical," and "sag" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal relative to "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0038] Please see Figure 1-8 A multi-stage filtration device for stem cell culture includes a device housing 1, a sealing cap 2, a double-layer movable filter assembly 3, and an movable adjustment structure 4 disposed on the surface of the device housing 1.

[0039] The double-layer movable filter assembly 3 includes a fixed sleeve 31 inside the device housing 1, a bottom filter 32 inside the fixed sleeve 31, an upper filter 33 on the surface of the bottom filter 32, and a filter frame 34 on the upper filter 33. The movable adjustment structure 4 includes an adjustment rod 41 on the surface of the sealing cover 2 and a pulling structure 42 on the surface of the filter frame 34. One end of the adjustment rod 41 extends into the device housing 1 and connects to the bottom filter 32 and the upper filter 33. The pulling structure 42 connects the filter frame 34 to the adjustment rod 41 and the fixed sleeve 31. When the adjustment rod 41 is pressed down, the upper filter 33 can... Under the action of the traction structure 42, a cone-shaped structure is formed. At this time, a gap is left between the upper filter screen 33 and the bottom filter screen 32 to allow cell fluid to flow in. When the adjusting rod 41 is pulled up, the upper filter screen 33 can fit into the bottom filter screen 32. When the flow rate of the upper filter screen 33 decreases due to the accumulation of cell debris, the operator pulls up the adjusting rod 41. The traction structure 42 is guided in the opposite direction by the connecting rope 422, so that the filter screen skeleton 34 is folded elastically, causing the upper filter screen 33 to flip up as a whole and fit tightly into the bottom filter screen 32. At this time, the accumulated cell fluid is directly introduced into the bottom filter screen 32 for secondary filtration through the opening between the fixing ring and the connecting structure 43 under the action of gravity, avoiding secondary cleaning.

[0040] In some embodiments, to ensure a stable connection between the adjusting rod 41 and the double-layer movable filter, a fixing ring is provided at one end of the adjusting rod 41 extending into the device housing. The bottom filter 32 and the upper filter 33 are both connected to the fixing ring. A filter is provided inside the fixing ring, and the filter frame 34 is a rod-shaped structure made of elastic material.

[0041] The tensioning structure 42 includes an elastic rope 421 connecting the end of the filter frame 34 and a connecting rope 422 connecting the end of the filter frame 34 to the inside of the fixing sleeve 31. The elastic rope 421 can ensure that the upper filter 33 forms a conical structure.

[0042] In other embodiments, a connecting structure 43 is provided between the fixing ring and the adjusting rod 41, and an opening is provided between the connecting structure 43 and the fixing ring to allow cell fluid to pass through. In order to ensure that the bottom filter screen 32 can stably support the adjusting rod 41, a limiting rope 423 is provided between the fixing ring and the fixing sleeve 31, and the limiting rope 423 is connected to the bottom filter screen 32.

[0043] To ensure the airtightness of the device and facilitate the smooth entry of cell fluid into the upper filter 33, the adjusting rod 41 has an inlet channel inside, an outlet on the surface of the adjusting rod 41 inside the device housing, an inlet at the end of the adjusting rod 41 extending to the outside of the device housing, an inlet hopper 5 at the inlet of the adjusting rod 41, and a sealing cap 6 at the opening of the inlet hopper 5. The sealing cap 6 can be attached to the inlet hopper 5 via a snap-fit ​​or locking structure. The surface of the sealing cap 6 has an operating handle, allowing the operator to manually operate the device after the cell fluid has been infused.

[0044] To ensure filtration accuracy, a bottom filter structure 7 may be provided inside the device housing. The bottom filter structure 7 includes a mesh frame and a secondary filter screen disposed inside the mesh frame.

[0045] In the description of this technology, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this technology based on the specific circumstances.

[0046] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A multi-stage filtration device for stem cell culture, comprising a device housing (1) and a sealing cap (2) disposed on the surface of the device housing (1), characterized in that, Also includes: The double-layer movable filter assembly (3) includes a fixed sleeve (31) disposed inside the device housing (1), a bottom filter (32) disposed inside the fixed sleeve (31), an upper filter (33) disposed on the surface of the bottom filter (32), and a filter skeleton (34) disposed on the upper filter (33). The movable adjustment structure (4) includes an adjustment rod (41) disposed on the surface of the sealing cover (2) and a pulling structure (42) disposed on the surface of the filter frame (34). One end of the adjustment rod (41) extends into the device housing (1) and is connected to the bottom filter (32) and the upper filter (33). The pulling structure (42) can connect the filter frame (34) to the adjustment rod (41) and the fixing sleeve (31). When the adjusting rod (41) is pressed down, the upper filter (33) can form a cone-shaped structure under the action of the pulling structure (42). At this time, there is a gap between the upper filter (33) and the bottom filter (32) for cell fluid to flow in. When the adjusting rod (41) is pulled up, the upper filter screen (33) can fit into the bottom filter screen (32).

2. The multi-stage filtration device for stem cell culture according to claim 1, characterized in that... The adjusting rod (41) has a fixing ring at one end extending into the device housing. The bottom filter (32) and the upper filter (33) are both connected to the fixing ring, and the fixing ring has a filter inside.

3. The multi-stage filtration device for stem cell culture according to claim 1, characterized in that... The filter frame (34) is a rod-shaped structure made of elastic material.

4. The multi-stage filtration device for stem cell culture according to claim 1, characterized in that... The tensioning structure (42) includes an elastic rope (421) connecting the end of the filter frame (34) and a connecting rope (422) connecting the end of the filter frame (34) to the inside of the fixing sleeve (31).

5. A multi-stage filtration device for stem cell culture according to claim 2, characterized in that... A connecting structure (43) is provided between the fixing ring and the adjusting rod (41), and an opening is provided between the connecting structure (43) and the fixing ring to allow cell fluid to pass through.

6. A multi-stage filtration device for stem cell culture according to claim 5, characterized in that... A limiting rope (423) is provided between the fixing ring and the fixing sleeve (31), and the limiting rope (423) is connected to the bottom filter screen (32).

7. A multi-stage filtration device for stem cell culture according to claim 1, characterized in that... The adjusting rod (41) has an inlet channel inside, and the surface of the adjusting rod (41) inside the device housing has an outlet. The end of the adjusting rod (41) extending to the outside of the device housing has an inlet.

8. A multi-stage filtration device for stem cell culture according to claim 1, characterized in that... The adjusting rod (41) is provided with a liquid inlet hopper (5) at the liquid inlet, and a sealing cap (6) is provided at the opening of the liquid inlet hopper (5).

9. A multi-stage filtration device for stem cell culture according to claim 8, characterized in that... The sealing cover (6) has an operating handle on its surface.

10. A multi-stage filtration device for stem cell culture according to claim 2, characterized in that... The device housing has a bottom filter structure (7) inside, which includes a mesh frame and a secondary filter screen inside the mesh frame.

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