An experimental device for producing antibacterial peptide bacillus fermentation

Abstract

The utility model discloses a kind of experimental devices for producing antibacterial peptide bacillus fermentation, belong to the technical field of microbial fermentation equipment, including bottom plate, the bottom plate is fixedly connected with support frame, the top of the support frame is fixedly connected with casing, the inside of the casing is fixedly connected with fermentation tank, temperature control interlayer is equipped between the fermentation tank and casing, temperature sensor is fixedly installed on the inner wall of the fermentation tank, stirring rod is rotatably installed in the inside of the fermentation tank, one side of the casing is equipped with nutrient storage tank, infusion pipeline is fixed between the bottom of the nutrient storage tank and casing, the infusion pipeline is penetrated to the inside of fermentation tank, the whole device of the utility model provides stable condition for producing antibacterial peptide bacillus high-density fermentation, improves spore yield and antibacterial peptide production, simultaneously provides high-quality fungicide support technical effect for subsequent probiotic characterization, broiler effectiveness evaluation.

Description

Technical Field

[0001] This utility model belongs to the field of microbial fermentation equipment technology, and in particular relates to an experimental device for fermentation of antimicrobial peptide-producing Bacillus. Background Technology

[0002] Bacillus spp. that produce antimicrobial peptides has significant value in the field of probiotics. Research on this topic needs to focus on the nutritional characterization of probiotic molecules, high-density fermentation preparation, and efficacy evaluation in young broilers. High-density fermentation is a key step, requiring precise control of temperature and nutrient supply to improve spore yield and antimicrobial peptide production.

[0003] However, existing experimental fermentation devices have significant drawbacks: insufficient temperature control precision, which can easily lead to abnormal bacterial metabolism and inhibited spore formation; inefficient nutrient addition, which cannot match the needs of different growth stages of the bacteria, resulting in low product yield; and lagging parameter monitoring and adjustment, which relies on manual operation and is prone to contamination, making it difficult to stabilize fermentation conditions. These device defects not only restrict the optimization of high-density fermentation processes but also result in a lack of sufficient high-quality bacterial strains for subsequent probiotic characterization studies and a lack of highly active bacterial agents for broiler evaluation experiments, becoming a bottleneck in the entire research process. Dedicated experimental devices are urgently needed to solve these problems. Utility Model Content

[0004] To address the problems existing in the prior art, this utility model provides an experimental device for the fermentation of antimicrobial peptide-producing Bacillus. The device provides stable conditions for high-density fermentation of antimicrobial peptide-producing Bacillus, improves spore yield and antimicrobial peptide production, and provides high-quality bacterial agent support for subsequent probiotic characterization and broiler efficacy evaluation. It solves the problems of insufficient temperature control precision, crude nutrient addition, lagging parameter monitoring and adjustment, and easy contamination during manual operation in existing experimental fermentation devices.

[0005] This invention is implemented as follows: an experimental apparatus for fermenting antimicrobial peptide-producing Bacillus includes a base plate, a support frame fixedly connected to the base plate, a casing fixedly connected to the top of the support frame, a fermenter fixedly connected inside the casing, a temperature-controlled interlayer between the fermenter and the casing, a temperature sensor fixedly installed on the inner wall of the fermenter, a stirring rod rotatably installed inside the fermenter, a PLC controller and a sampling port fixedly installed on the front of the casing, the sampling port penetrating into the interior of the fermenter, a temperature-controlled water tank fixedly installed on the corresponding base plate below the casing, the two sides of the temperature-controlled water tank being connected to the temperature-controlled interlayer via inlet and outlet pipes, a nutrient storage tank on one side of the casing, and a delivery pipeline fixed between the bottom of the nutrient storage tank and the casing, the delivery pipeline penetrating into the interior of the fermenter.

[0006] As a preferred embodiment of this invention, a pH sensor and a turbidity sensor are fixedly installed on the top of the fermenter, a dissolved oxygen sensor is fixedly installed on the side wall of the fermenter, and a reserved channel for the dissolved oxygen sensor to pass through is provided on the casing. The pH sensor, turbidity sensor, dissolved oxygen sensor and temperature sensor are all electrically connected to the PLC controller.

[0007] This setup allows for real-time collection of key parameters in the fermentation broth, including pH, cell density (turbidity), dissolved oxygen, and temperature. The data is then transmitted to the PLC controller, enabling parameter visualization and automatic feedback adjustment. This avoids the lag and contamination risks associated with manual monitoring, provides stable conditions for strain metabolism, and ensures spore yield and antimicrobial peptide production.

[0008] As a preferred embodiment of this utility model, a coil is fixedly installed on the temperature control interlayer, and an inlet pipe and an outlet pipe are fixedly connected to the upper and lower ends of the coil, respectively. A water pump is fixedly installed on the temperature control water tank, and the outlet end of the water pump is fixedly connected to the bottom of the inlet pipe. The top side wall of the temperature control water tank is fixedly connected to the bottom of the outlet pipe.

[0009] With this setting, the temperature control medium in the temperature-controlled water tank is deionized water, which allows the temperature control medium to circulate evenly along the coil, expands the contact area with the fermenter, improves the uniformity of temperature distribution inside the tank, avoids local temperature deviations that could lead to abnormal bacterial metabolism, and solves the problem of uneven temperature control in existing devices.

[0010] As a preferred embodiment of this utility model, a cooler and an electric heating rod are fixedly installed at the bottom of the temperature-controlled water tank, and the cooler, the electric heating rod and the water pump are all electrically connected to the PLC controller.

[0011] With this setting, the PLC controller can automatically start and stop the cooler and electric heating rod according to the temperature sensor signal, quickly adjust the temperature of the temperature control medium, and at the same time control the water pump to ensure the efficiency of medium circulation, so as to achieve precise and stable temperature of fermentation liquid and prevent spore formation from being hindered.

[0012] In a preferred embodiment of this invention, the nutrient storage tank and the base plate are fixedly connected by a support plate. At least three infusion lines are fixedly installed at the bottom of the nutrient storage tank. Evenly distributed partitions are fixedly connected inside the nutrient storage tank, and the partitions and infusion lines are staggered. A feed hopper is fixedly installed at the top of the nutrient storage tank, and the feed hopper and infusion lines are correspondingly arranged. A solenoid valve is fixedly installed at the upper part of the infusion line, and a metering pump is fixedly installed at the lower part of the infusion line. Both the solenoid valve and the metering pump are electrically connected to a PLC controller.

[0013] This design allows the nutrient storage tank to be divided into multiple chambers, which store different nutrients such as carbon sources, nitrogen sources, and inorganic salts. The PLC controller controls the opening and closing of the channels through solenoid valves, and in conjunction with the metering pump, it precisely regulates the supply of each nutrient, enabling on-demand feeding to match the needs of different growth stages of the bacteria and avoid nutrient imbalance that leads to low product yield.

[0014] In a preferred embodiment of this invention, a support platform is fixedly connected to the bottom of the fermentation tank, and a stirring rod is connected between the support platform and the top of the fermentation tank via a bearing. Stirring blades are sleeved and fixed to the side wall of the stirring rod, and a motor is fixedly installed on the top of the fermentation tank. The output end of the motor is fixedly connected to the upper end of the stirring rod.

[0015] With this setup, the motor drives the stirring rod to rotate the stirring blades, which allows the fermentation broth, bacteria, nutrients and oxygen to be fully mixed, improving dissolved oxygen utilization and nutrient contact efficiency, reducing the problem of local nutrient excess or insufficient dissolved oxygen, promoting efficient metabolism of the strains, and facilitating high-density fermentation.

[0016] Compared with existing technologies, the beneficial effects of this invention are as follows: First, the temperature-controlled jacket with coils, a cooler, and an electric heating rod, combined with PLC automatic temperature control, achieves precise and stable fermentation broth temperature, avoiding abnormal bacterial metabolism and hindered spore formation. Second, the nutrient storage tank with divided compartments and multi-pipeline metering pumps can supply carbon and nitrogen sources as needed, matching the needs of different growth stages of the bacteria and preventing nutrient imbalance. Third, multiple sensors collect parameters such as pH and dissolved oxygen in real time, and the PLC automatically provides feedback and adjustment, reducing manual contamination and monitoring lag. Fourth, the stirring system improves mixing efficiency and ensures dissolved oxygen and nutrient utilization. The overall device provides stable conditions for high-density fermentation of antimicrobial peptide-producing Bacillus, improving spore yield and antimicrobial peptide production, while providing high-quality bacterial agent support for subsequent probiotic characterization and broiler efficacy evaluation. Attached Figure Description

[0017] Figure 1 This is a three-dimensional structural schematic diagram provided in an embodiment of the present utility model; Figure 2 This is a schematic diagram of the bottom structure provided in an embodiment of the present utility model; Figure 3 This is a cross-sectional structural schematic diagram provided in an embodiment of the present utility model; Figure 4 This is a schematic diagram of the nutrient storage tank structure provided in an embodiment of this utility model.

[0018] In the diagram: 1. Base plate; 101. Support frame; 102. Support plate; 2. Fermentation tank; 201. Sampling port; 202. pH sensor; 203. Turbidity sensor; 204. Dissolved oxygen sensor; 205. Temperature sensor; 3. Housing; 301. Temperature control jacket; 4. Temperature control water tank; 401. Water pump; 402. Coil; 403. Inlet pipe; 404. Outlet pipe; 405. Refrigerator; 406. Electric heating rod; 5. Stirring rod; 501. Stirring blade; 502. Motor; 503. Support platform; 6. Nutrient storage tank; 601. Feed hopper; 602. Baffle; 603. Infusion pipeline; 604. Metering pump; 605. Solenoid valve; 7. PLC controller. Detailed Implementation

[0019] To further understand the utility model content, features and effects of this utility model, the following embodiments are provided, and detailed descriptions are given in conjunction with the accompanying drawings.

[0020] The structure of this utility model will now be described in detail with reference to the accompanying drawings.

[0021] refer to Figures 1 to 4 As shown in the figure, an experimental apparatus for fermenting antimicrobial peptide-producing Bacillus spores according to an embodiment of this utility model includes a base plate 1, a support frame 101 fixedly connected to the base plate 1, a casing 3 fixedly connected to the top of the support frame 101, a fermentation tank 2 fixedly connected inside the casing 3, a temperature control interlayer 301 provided between the fermentation tank 2 and the casing 3, a temperature sensor 205 fixedly installed on the inner wall of the fermentation tank 2, and a stirring rod 5 rotatably installed inside the fermentation tank 2. A PLC controller 7 and a sampling port 201 are fixedly installed on the surface of the fermenter 2. The sampling port 201 extends into the interior of the fermenter 2. A temperature-controlled water tank 4 is fixedly installed on the bottom plate 1 below the casing 3. The two sides of the temperature-controlled water tank 4 are connected to the temperature-controlled interlayer 301 through the inlet pipe 403 and the outlet pipe 404. A nutrient storage tank 6 is provided on one side of the casing 3. A delivery pipeline 603 is fixed between the bottom of the nutrient storage tank 6 and the casing 3. The delivery pipeline 603 extends into the interior of the fermenter 2.

[0022] PLC, or Programmable Logic Controller, is a digital computing electronic system designed specifically for industrial environments.

[0023] Specifically, a pH sensor 202 and a turbidity sensor 203 are fixedly installed on the top of the fermentation tank 2, and a dissolved oxygen sensor 204 is fixedly installed on the side wall of the fermentation tank 2. A reserved channel for the dissolved oxygen sensor 204 to pass through is provided on the housing 3. The pH sensor 202, turbidity sensor 203, dissolved oxygen sensor 204 and temperature sensor 205 are all electrically connected to the PLC controller 7.

[0024] Using the above scheme, key parameters such as pH, cell density, turbidity, dissolved oxygen, and temperature of the fermentation broth can be collected in real time and transmitted to the PLC controller 7. This enables parameter visualization and automatic feedback adjustment, avoiding the lag and contamination risks of manual monitoring, providing stable conditions for strain metabolism, and ensuring spore yield and antimicrobial peptide production.

[0025] Specifically, a coil 402 is fixedly installed on the temperature-controlled interlayer 301, and an inlet pipe 403 and an outlet pipe 404 are fixedly connected to the upper and lower ends of the coil 402, respectively. A water pump 401 is fixedly installed on the temperature-controlled water tank 4, and the outlet end of the water pump 401 is fixedly connected to the bottom of the inlet pipe 403. The top side wall of the temperature-controlled water tank 4 is fixedly connected to the bottom of the outlet pipe 404.

[0026] Using the above scheme, the temperature control medium in the temperature control tank 4 is deionized water, which allows the temperature control medium to circulate evenly along the coil 402, expands the contact area with the fermenter 2, improves the uniformity of temperature distribution inside the tank, avoids local temperature deviations that cause abnormal bacterial metabolism, and solves the problem of uneven temperature control in existing devices.

[0027] Specifically, a cooler 405 and an electric heating rod 406 are fixedly installed at the bottom of the temperature-controlled water tank 4, and the cooler 405, the electric heating rod 406 and the water pump 401 are all electrically connected to the PLC controller 7.

[0028] Using the above scheme, the PLC controller 7 can automatically start and stop the cooler 405 and the electric heating rod 406 according to the signal of the temperature sensor 205, quickly adjust the temperature of the temperature control medium, and at the same time control the water pump 401 to ensure the efficiency of medium circulation, so as to achieve precise and stable temperature of fermentation liquid and prevent spore formation from being hindered.

[0029] Specifically, the nutrient storage tank 6 and the base plate 1 are fixedly connected by a support plate 102. At least three infusion lines 603 are fixedly installed at the bottom of the nutrient storage tank 6. Evenly distributed partitions 602 are fixedly connected inside the nutrient storage tank 6. The partitions 602 and the infusion lines 603 are staggered. A feed hopper 601 is fixedly installed at the top of the nutrient storage tank 6. The feed hopper 601 and the infusion lines 603 are correspondingly arranged. A solenoid valve 605 is fixedly installed at the upper part of the infusion line 603. A metering pump 604 is fixedly installed at the lower part of the infusion line 603. Both the solenoid valve 605 and the metering pump 604 are electrically connected to the PLC controller 7.

[0030] Using the above scheme, the partition 602 can divide the nutrient storage tank 6 into multiple chambers to store different nutrients such as carbon source, nitrogen source, and inorganic salts respectively; the PLC controller 7 controls the opening and closing of the channel through the solenoid valve 605, and in combination with the metering pump 604, it precisely regulates the supply of each nutrient to achieve on-demand feeding, match the needs of different growth stages of the bacteria, and avoid low product yield due to nutrient imbalance.

[0031] Specifically, a support platform 503 is fixedly connected to the bottom of the fermentation tank 2. A stirring rod 5 is connected between the support platform 503 and the top of the fermentation tank 2 via a bearing. A stirring blade 501 is sleeved and fixed to the side wall of the stirring rod 5. A motor 502 is fixedly installed on the top of the fermentation tank 2. The output end of the motor 502 is fixedly connected to the upper end of the stirring rod 5.

[0032] Using the above scheme, the motor 502 drives the stirring rod 5 to rotate the stirring blade 501, which can fully mix the fermentation broth, bacteria, nutrients and oxygen, improve dissolved oxygen utilization and nutrient contact efficiency, reduce the problem of local nutrient excess or insufficient dissolved oxygen, promote efficient metabolism of the strain, and help high-density fermentation.

[0033] The working principle of this utility model: When in use, the pH sensor 202 is a Mettler Toledo InPro 3250i / 12 / 120; the turbidity sensor 203 is a Mettler Toledo InPro 8200 / S / Epoxy / 407; the dissolved oxygen sensor 204 is a Mettler Toledo InPro 6800 / 12 / 070; the temperature sensor 205 is a Wika TR10-C; the metering pump 604 is a Milton Roy AA766-y; the motor 502 is a SEW-EURODRIVE MOVIMOT® advanced 7.5kW; the water pump 401 is a Grundfos CR5-12; the solenoid valve 605 is an ASCO 327 series 8327B002; and the PLC controller 7 is a Siemens S7-200 SMARTCPU SR60.

[0034] Temperature control start-up: PLC controller 7 automatically starts and stops the cooler 405 and electric heating rod 406 in temperature control water tank 4 according to the signal of temperature sensor 205 on the inner wall of fermentation tank 2, and adjusts the temperature of deionized water; water pump 401 pumps the temperature control medium into the coil 402 of temperature control jacket 301, and circulates it evenly along the tank wall to maintain the stable temperature of fermentation liquid.

[0035] Nutritional supply: The nutrient storage tank 6 is divided into compartments by partitions 602 to store carbon sources, nitrogen sources, etc. The PLC, combined with the turbidity sensor 203, reflects the cell density data and controls the on / off state of the solenoid valve 605 of the corresponding infusion pipeline 603 and the speed of the metering pump 604 to accurately replenish nutrients into the tank as needed, matching the needs of the cell growth stage.

[0036] Mixing and dissolved oxygen optimization: Motor 502 drives stirring rod 5 to rotate the blades, so that the fermentation broth, bacteria, nutrients and oxygen are fully mixed, improving dissolved oxygen utilization and avoiding uneven distribution of nutrients and dissolved oxygen in some areas.

[0037] Real-time monitoring and adjustment: pH, dissolved oxygen, and turbidity sensors 203 collect data in real time and transmit it to the PLC. If dissolved oxygen is insufficient, the stirring speed is increased. If pH is abnormal, the corresponding nutrients are added to achieve automatic feedback adjustment of parameters. Sampling port 201 can take samples aseptically to help verify the fermentation effect and ultimately ensure spore yield and antimicrobial peptide production.

[0038] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0039] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An experimental apparatus for fermentation of antimicrobial peptide-producing Bacillus, comprising a base plate (1), characterized in that: A support frame (101) is fixedly connected to the base plate (1). A casing (3) is fixedly connected to the top of the support frame (101). A fermentation tank (2) is fixedly connected inside the casing (3). A temperature control interlayer (301) is provided between the fermentation tank (2) and the casing (3). A temperature sensor (205) is fixedly installed on the inner wall of the fermentation tank (2). A stirring rod (5) is rotatably installed inside the fermentation tank (2). A PLC controller (7) and a sampling port (201) are fixedly installed on the front of the casing (3). The sampling port (201) extends into the interior of the fermenter (2). A temperature-controlled water tank (4) is fixedly installed on the bottom plate (1) below the casing (3). The two sides of the temperature-controlled water tank (4) are connected to the temperature-controlled interlayer (301) through the inlet pipe (403) and the outlet pipe (404). A nutrient storage tank (6) is provided on one side of the casing (3). A delivery pipeline (603) is fixed between the bottom of the nutrient storage tank (6) and the casing (3). The delivery pipeline (603) extends into the interior of the fermenter (2).

2. The experimental apparatus for fermentation of antimicrobial peptide-producing Bacillus as described in claim 1, characterized in that: A pH sensor (202) and a turbidity sensor (203) are fixedly installed on the top of the fermentation tank (2), and a dissolved oxygen sensor (204) is fixedly installed on the side wall of the fermentation tank (2). A reserved channel for the dissolved oxygen sensor (204) to pass through is provided on the casing (3). The pH sensor (202), turbidity sensor (203), dissolved oxygen sensor (204) and temperature sensor (205) are all electrically connected to the PLC controller (7).

3. The experimental apparatus for fermentation of antimicrobial peptide-producing Bacillus as described in claim 1, characterized in that: A coil (402) is fixedly installed on the temperature control interlayer (301). An inlet pipe (403) and an outlet pipe (404) are fixedly connected to the upper and lower ends of the coil (402), respectively. A water pump (401) is fixedly installed on the temperature control water tank (4). The outlet end of the water pump (401) is fixedly connected to the bottom of the inlet pipe (403). The top side wall of the temperature control water tank (4) is fixedly connected to the bottom of the outlet pipe (404).

4. The experimental apparatus for fermentation of antimicrobial peptide-producing Bacillus as described in claim 3, characterized in that: The bottom of the temperature-controlled water tank (4) is fixedly equipped with a cooler (405) and an electric heating rod (406), and the cooler (405), the electric heating rod (406) and the water pump (401) are all electrically connected to the PLC controller (7).

5. The experimental apparatus for fermentation of antimicrobial peptide-producing Bacillus as described in claim 1, characterized in that: The nutrient storage tank (6) and the base plate (1) are fixedly connected by a support plate (102). At least three infusion lines (603) are fixedly installed at the bottom of the nutrient storage tank (6). Evenly distributed partitions (602) are fixedly connected inside the nutrient storage tank (6). The partitions (602) and the infusion lines (603) are staggered. A feed hopper (601) is fixedly installed at the top of the nutrient storage tank (6). The feed hopper (601) and the infusion lines (603) are correspondingly arranged. A solenoid valve (605) is fixedly installed at the upper part of the infusion line (603). A metering pump (604) is fixedly installed at the lower part of the infusion line (603). The solenoid valve (605) and the metering pump (604) are both electrically connected to the PLC controller (7).

6. The experimental apparatus for fermentation of antimicrobial peptide-producing Bacillus as described in claim 1, characterized in that: A support platform (503) is fixedly connected to the bottom of the fermentation tank (2). A stirring rod (5) is connected between the support platform (503) and the top of the fermentation tank (2) via a bearing. A stirring blade (501) is sleeved and fixed to the side wall of the stirring rod (5). A motor (502) is fixedly installed on the top of the fermentation tank (2). The output end of the motor (502) is fixedly connected to the upper end of the stirring rod (5).

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