A root exudate collection device

Abstract

The utility model relates to the technical field of plant ecology research provides a kind of root system secretion collection device, including the bottle, the bottom of bottle is provided with outlet pipe for connecting the collection bottle of outside, the top of bottle is also provided with sealing plug one pair of bottle is closed, the top of sealing plug one is provided with two slot holes respectively connected with water injection pipe and plant root system;Filtering assembly, filtering assembly is set in the bottom and inside of bottle, filtering assembly includes mesh funnel and funnel-shaped collection pipe, funnel-shaped collection pipe is semicircular and is installed in the bottom of bottle;Through the root system secretion collection device through the optimized collection assembly design, including funnel-shaped collection pipe and the collection bottle being communicated therewith, cooperate the negative pressure environment formed by air pump, can efficiently collect root system secretion into collection bottle, greatly improve the collection efficiency.

Description

Technical Field

[0001] This utility model relates to the field of plant ecological research technology, specifically a root exudate collection device. Background Technology

[0002] Root exudates play a crucial role in the interaction between plants and the soil environment, and their study contributes to a deeper understanding of plant physiological and ecological processes, nutrient absorption mechanisms, and plant-microbe interactions. However, the accurate and efficient collection of root exudates remains a technical challenge in this research field.

[0003] Traditional methods for collecting root exudates have several shortcomings. For example, some methods cannot avoid the degradation and contamination of exudates by soil microorganisms, leading to changes in the composition of the collected exudates and affecting subsequent analysis and research results. In addition, some methods are prone to causing root damage during operation, which in turn affects the normal physiological activities of plants, making the collected exudates unable to accurately reflect the plant's secretion under natural conditions.

[0004] To address the problems raised in the background art, those skilled in the art have proposed a root exudate collection device. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a root exudate collection device that can accurately control the root growth environment, flexibly fix the root system, realize in-situ efficient collection of exudates, and is easy to operate.

[0006] A root exudate collection device includes a placement bottle, the bottom of which is provided with a water outlet pipe for connecting to an outer collection bottle, and the top of which is also provided with a sealing plug to seal the placement bottle. The top of the sealing plug has two slots that are respectively connected to a water inlet pipe and a plant root system.

[0007] A filter assembly is disposed at the bottom and inside of the placement bottle. The filter assembly includes a mesh funnel and a funnel-shaped collection tube. The funnel-shaped collection tube is semi-circularly installed at the bottom of the placement bottle. A mesh funnel is installed between the funnel-shaped collection tube and the placement bottle. The bottom end of the funnel-shaped collection tube is also connected to a water outlet pipe.

[0008] Preferably, a second sealing plug is installed at the top of the collection bottle, and the top of the second sealing plug has two openings that are respectively connected to a water outlet pipe and a suction pipe. A suction pump is installed at the end of the suction pipe away from the second sealing plug.

[0009] Preferably, the placement bottle is also provided with an acid-washed glass ball.

[0010] Preferably, a water-stop clamp is provided on the outside of the water injection pipe and the water outlet pipe to close or open the water injection pipe and the water outlet pipe.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] 1. The root exudate collection device, through its optimized collection component design, including a funnel-shaped collection tube and a collection bottle connected to it, combined with the negative pressure environment created by the air pump, can efficiently collect root exudates into the collection bottle, greatly improving collection efficiency. Attached Figure Description

[0013] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0014] Figure 2 This utility model Figure 1 A cross-sectional view of the bottle placed in the middle;

[0015] Figure 3 This utility model Figure 2 A schematic diagram showing the specific structure of the bottle placed in the middle.

[0016] In the diagram: 1. Placement bottle; 2. Sealing plug one; 3. Water inlet pipe; 4. Root placement trough; 5. Water outlet pipe; 6. Sealing plug two; 7. Collection bottle; 8. Vacuum pipe; 10. Vacuum pump; 11. Mesh funnel; 12. Funnel-shaped collection pipe. Detailed Implementation

[0017] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0018] As attached Figure 1 To be continued Figure 3 As shown:

[0019] Example 1: According to Figures 1-3 As shown, this utility model provides a root exudate collection device, including a placement bottle 1. The bottom end of the placement bottle 1 is provided with a water outlet pipe 5 for connecting to an outer collection bottle 7. The top of the placement bottle 1 is also provided with a sealing plug 2 to seal the placement bottle 1. The top of the sealing plug 2 has two slots that are respectively connected to a water inlet pipe 3 and the plant roots.

[0020] A filter assembly is disposed at the bottom and inside of the placement bottle 1. The filter assembly includes a mesh funnel 11 and a funnel-shaped collection tube 12. The funnel-shaped collection tube 12 is semi-circularly installed at the bottom of the placement bottle 1. The mesh funnel 11 is installed between the funnel-shaped collection tube 12 and the placement bottle 1. The bottom end of the funnel-shaped collection tube 12 is also connected to the water outlet pipe 5. A sealing plug 6 is installed at the top of the collection bottle 7. The top of the sealing plug 6 has two openings that are respectively connected to the water outlet pipe 5 and the suction pipe 8. A suction pump 10 is installed at the end of the suction pipe 8 away from the sealing plug 6. An acid-washed glass ball is also disposed inside the placement bottle 1. A water-stop clamp is provided on the outside of the water injection pipe 3 and the water outlet pipe 5 to close or open the water injection pipe 3 and the water outlet pipe 5.

[0021] The mesh funnel 11 is made of PET polyester fiber material with a pore size of 32 micrometers.

[0022] Temperature sensors, humidity sensors, and light intensity sensors can be installed inside the collection container to monitor environmental parameters inside the container in real time.

[0023] A heating belt and cooling pads are installed around the outer wall of the collection container as a temperature control device, a spray device is connected through pipes as a humidity control device, and an LED light panel is installed on top of the collection container as a light control device. All control devices are electrically connected to a controller, which controls them based on monitored environmental parameters.

[0024] Working principle: When this device is needed, first carefully dig out 1-2 fine roots connected to the base in the 0-20 cm soil layer, rinse the fine roots with deionized water to remove the root surface soil, place the culture medium in the placement bottle 1, and then put the intact fine roots into the placement bottle 1. Insert the plant roots into the root placement groove 4, then start the suction pump 10. The suction pump 10 extracts the gas in the collection bottle 7 through the suction pipe 8, creating a negative pressure in the collection bottle 7. This causes the root secretions to pass through the mesh funnel 11 in situ, and then through the funnel-shaped collection pipe 12 and the water outlet pipe into the collection bottle 7.

[0025] All standard parts used in this invention can be purchased from the market, and irregularly shaped parts can be customized according to the description and drawings. The specific connection methods for each part all employ conventional methods such as bolts, rivets, and welding, which are mature technologies in the prior art. The machinery, parts, and equipment all use conventional models in the prior art, and the circuit connections also use conventional connection methods in the prior art, which will not be detailed here. Any content not described in detail in this specification belongs to the prior art known to those skilled in the art.

[0026] In the description of this utility model, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. "A plurality of" means two or more, unless otherwise explicitly specified.

[0027] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0028] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0029] In the description of this specification, the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0030] The accompanying drawings of the embodiments disclosed in this utility model only involve the structures involved in the embodiments disclosed in this utility model. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of this utility model can be combined with each other.

[0031] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A root exudate collection device, characterized by: Includes a placement bottle (1), the bottom of which is provided with a water outlet pipe (5) for connecting to an outer collection bottle (7), and the top of the placement bottle (1) is also provided with a sealing plug (2) to seal the placement bottle (1). The top of the sealing plug (2) has two slots that are respectively connected to the water injection pipe (3) and the plant roots. The filter assembly is disposed at the bottom and inside of the placement bottle (1). The filter assembly includes a mesh funnel (11) and a funnel-shaped collection tube (12). The funnel-shaped collection tube (12) is semi-circularly installed at the bottom of the placement bottle (1). The mesh funnel (11) is installed between the funnel-shaped collection tube (12) and the placement bottle (1). The bottom end of the funnel-shaped collection tube (12) is also connected to the water outlet pipe (5).

2. The root exudate collection device of claim 1, wherein: The top of the collection bottle (7) is fitted with a sealing plug 2 (6). The top of the sealing plug 2 (6) has two openings that are connected to a water outlet pipe (5) and a suction pipe (8) respectively. A suction pump (10) is installed at the end of the suction pipe (8) away from the sealing plug 2 (6).

3. The root exudate collection device as described in claim 1, characterized in that: The placement bottle (1) is also equipped with an acid-washed glass ball.

4. The root exudate collection device as described in claim 1, characterized in that: Water-stop clamps are provided on the outside of the water injection pipe (3) and the water outlet pipe (5) to close or open the water injection pipe (3) and the water outlet pipe (5).

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