A xanthan gum content decarboxylation method detection device

Abstract

The utility model discloses a kind of xanthan gum content decarboxylation method detection equipment, including at least three groups of following detection device, three groups of detection device are used for blank experiment, standard sample experiment, parallel experiment group experiment;The detection device includes two mouth flask, heating jacket, condenser, cylindrical funnel, gas washing bottle arranged in sequence;Among them, heating jacket, two mouth flask is placed in heating jacket, for the heating or cooling of two mouth flask;Condenser, one end of condenser is connected with the opening of two mouth flask, another end of condenser is communicated with one end of cylindrical funnel by first hose;Cylindrical funnel.The beneficial effects of the utility model are: shorten detection time (3 days required by traditional method is compressed to about 3 hours), improve detection efficiency.

Description

Technical Field

[0001] This utility model relates to the field of xanthan gum detection technology, and in particular to a xanthan gum content decarboxylation method detection device. Background Technology

[0002] Xanthan gum is a polysaccharide polymer produced through microbial fermentation. It appears as a white or pale yellow powder and has excellent water solubility, dissolving rapidly in both hot and cold water to form a high-viscosity solution. It is widely used as a thickener, stabilizer, and emulsifier in the food industry, including dairy products, condiments, baked goods, and beverages. It is also commonly found in the cosmetics and pharmaceutical industries.

[0003] Currently, the following methods are commonly used to detect xanthan gum content: After the sample was pretreated with low-concentration potassium hydroxide and hydrochloric acid solution, it was precipitated with anhydrous ethanol, and then washed multiple times with anhydrous ethanol and acetone to remove impurities. After filtration, the filter residue was dried and weighed, and the xanthan gum content was finally calculated.

[0004] The method has the following limitations: 1) The reaction time, stirring intensity and direction need to be precisely controlled during the operation, the error tolerance is low and the probability of experimental failure is high; 2) The fibrous structure of xanthan gum itself is easily affected by the accuracy of the detection results; 3) At the same time, the method relies on a large amount of anhydrous ethanol reagent, the overall process takes a long time (usually 3 days) and is costly.

[0005] In summary, this invention presents a xanthan gum content decarboxylation method detection device that overcomes the aforementioned shortcomings. Utility Model Content

[0006] The purpose of this invention is to address the shortcomings of existing technologies by proposing a xanthan gum content decarboxylation method detection device. This device overcomes the deficiencies of existing technologies and has the following advantages: shortening the detection time (reducing the time required by traditional methods from 3 days to about 3 hours) and reducing the overall detection cost.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: A xanthan gum content decarboxylation method detection device includes at least three sets of the following detection devices, which are used for blank experiments, standard sample experiments, and parallel experimental group experiments, respectively. The detection device includes a two-necked flask, a heating mantle, a condenser, a cylindrical funnel, and a gas washing bottle arranged sequentially. The heating mantle houses the two-necked flask for heating or cooling. The condenser has one end connected to one opening of the two-necked flask and the other end connected to one end of the cylindrical funnel via a first flexible tube. The cylindrical funnel is connected to the gas washing bottle. The gas washing bottle contains a glass tube, one end of which extends into the bottle, and the other end extends to the outside of the bottle and is connected to the cylindrical funnel via a second flexible tube.

[0008] As a further preferred embodiment, the condenser tube is equipped with a cooling water source interface for connecting and circulating cooling water.

[0009] As a further preferred embodiment, a gas supply device is also included.

[0010] As a further preferred embodiment, the gas supply device mainly includes a nitrogen cylinder, a flow meter, a first delivery pipe, and a second delivery pipe.

[0011] As a further preferred embodiment, the flow meter is mounted on the first delivery pipe; one end of the first delivery pipe is connected to the outlet of the nitrogen cylinder, and the other end is connected to the inlet of the flow meter; the second delivery pipe is connected to the outlet of the flow meter at one end, and the other end is connected to a two-necked flask.

[0012] This utility model has the following beneficial effects: 1) This utility model, through the setting of multi-station heating and reaction devices, such as three sets of detection devices for blank experiments, standard sample experiments and parallel experimental group experiments respectively, can simultaneously carry out blank, standard sample and sample (parallel test) tests, ensuring the stability of each round of test results as well as the accuracy and timeliness of the results; 2) The test was completed using the xanthan gum content decarboxylation method detection equipment, and multiple stations could be operated simultaneously, making the process easy to control and achieving a high success rate.

[0013] 3) When conducting tests with this device, only solutions such as hydrochloric acid and sodium hydroxide are used; organic solvents such as anhydrous ethanol and acetone are not used.

[0014] 4) The detection cycle has been significantly shortened, reducing the time required by traditional methods from 3 days to about 3 hours, thus significantly improving detection efficiency.

[0015] By using chemical methods, the xanthan gum content is quantitatively calculated based on the carbon dioxide produced by the decarboxylation reaction. The content of xanthan gum is calculated by calculating the CO2 produced by the chemical reaction, resulting in a more accurate result. Attached Figure Description

[0016] Figure 1 This is a perspective view of a xanthan gum content decarboxylation detection device according to the present invention. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Referring to the accompanying drawings, this utility model provides a xanthan gum content decarboxylation method detection device.

[0019] A xanthan gum content decarboxylation method detection device includes at least three sets of the following detection devices, which are respectively used for blank experiment, standard sample experiment and parallel experimental group experiment; The detection device comprises, in sequence: a two-necked flask 40, a heating mantle 30, a condenser 50, a cylindrical funnel 80, and a gas washing bottle 10; in, Heating jacket 30, two-necked flask 40 is placed inside heating jacket 30 for heating or cooling two-necked flask 40; The condenser 50 has one end connected to one opening of the two-necked flask 40, and the other end connected to one end of the cylindrical funnel 80 via a flexible tube 51. Cylindrical funnel 80, which is connected to a gas washing bottle; Gas washing bottle 10, with a glass tube 11 inside. One end of the glass tube 11 extends into the interior of the washing bottle 10, and the other end extends to the exterior of the washing bottle 10 and is connected to the cylindrical funnel 80 through a flexible tube 52.

[0020] As a further preferred embodiment, the condenser tube 50 is equipped with a cooling water source interface 70 for connecting and circulating cooling water.

[0021] In this embodiment, the cooling water source is connected to the condenser tube 50 through interface 70 to form a circulating cooling loop. The cooling water flows into the outer jacket of the condenser tube 50 from the inlet of interface 70, flows around the internal steam channel and continuously absorbs heat, and is finally discharged from the outlet, thereby achieving efficient condensation and reflux of steam.

[0022] As a further preferred embodiment, it also includes a gas supply device 60.

[0023] As a further preferred embodiment, the gas supply device 60 mainly includes a nitrogen cylinder 61, a flow meter 62, a first delivery pipe 63, and a second delivery pipe 64.

[0024] As a further preferred embodiment, the flow meter 62 is disposed on the first delivery pipe 63; one end of the first delivery pipe 63 is connected to the outlet of the nitrogen cylinder 61, and the other end is connected to the inlet of the flow meter 62; the second delivery pipe 64 is connected to the outlet of the flow meter 62 at one end, and the other end is connected to the two-necked flask 40.

[0025] The above structure establishes a complete gas path from the nitrogen source to the reaction vessel.

[0026] In this embodiment, The gas supply device 60 mainly includes a nitrogen cylinder 61, a flow meter 62, a first delivery pipe 63, and a second delivery pipe 64. The flow meter 62 is mounted on the first delivery pipe 63; one end of the first delivery pipe 63 is connected to the outlet of the nitrogen cylinder 61, and the other end is connected to the inlet of the flow meter 62; the second delivery pipe 64 is connected to the outlet of the flow meter 62 at one end, and the other end is connected to a two-necked flask 40, thus establishing a complete gas path from the nitrogen source to the reaction vessel.

[0027] More specifically, the gas supply device 60 continuously supplies high-purity N2 to the two-necked flask 40 via a nitrogen cylinder 61. A flow meter 62 is used to monitor and precisely adjust the N2 flow rate to ensure stable and reliable atmosphere control. The specific airflow path is as follows: after flowing out of the nitrogen cylinder 61, N2 enters the flow meter 62 via a first delivery pipe 63, and after metering and adjustment, it is delivered to the inside of the two-necked flask 40 via a second delivery pipe 64.

[0028] Furthermore, the gas supply device 60 can be expanded and connected in series according to experimental needs. By distributing pipelines, it can simultaneously supply gas to three or more detection devices, serving blank experiments, standard sample experiments, and parallel experimental groups, thereby improving experimental efficiency.

[0029] To address the problems in existing technologies, this invention aims to remove the carboxyl groups from the sample using hydrochloric acid, then use nitrogen (N2) to carry the generated gas into a sodium hydroxide solution, where CO2 is converted into Na2CO3. Subsequently, barium chloride solution is added to convert Na2CO3 into BaCO3 to stabilize the CO2. Using the molecular weight of the repeating units of the biopolymer and the number of uronic acid groups in each repeating unit, the amount of CO2 released can be used to calculate the biogum content in the sample. The amount of NaOH consumed is then determined by titration with dilute hydrochloric acid, thus yielding the amount of CO2 generated, which is then converted into xanthan gum content.

[0030] As shown in the figure, a multi-station heating instrument can simultaneously perform blank, standard sample, and sample (parallel testing) tests, which can fully ensure the stability, accuracy, and timeliness of the test results in each round.

[0031] The specific steps are as follows: S1: Take 46 mL of concentrated hydrochloric acid and 10 drops of n-butanol, add to 40, and shake gently. Turn on the gas supply device 60, adjust the flow meter 20, and adjust the gas flow rate to 0.2 L / min. Turn on the cooling water supply 70 and maintain for 5 minutes. Remove air from the pipeline.

[0032] S2: Take 75 mL of 0.1 mol / L NaOH standard solution and put it into gas washing bottle 10. Take the sample and put it into a two-necked flask 40 containing hydrochloric acid. Turn on the heating function of heating mantle 30, set the power to 500W, heat to boiling in 10 minutes, adjust the power to 350W, and maintain for 2 hours.

[0033] S3: Turn on the cooling function of heating jacket 30, adjust the flow meter 20, and cool for 10 minutes to room temperature.

[0034] S4: Remove gas washing bottle 10, rinse the bottle wall and glass tube with deionized water, fix CO2 with barium chloride solution, titrate the remaining sodium hydroxide solution in gas washing bottle 10 with hydrochloric acid, calculate the CO2 content, and then calculate the xanthan gum content.

[0035] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A device for detecting the content of xanthan gum by decarboxylation method, characterized in that: The device includes at least three sets of the following detection apparatus, which are used for blank experiments, standard sample experiments, and parallel experimental group experiments, respectively. The detection apparatus includes, in sequence: a two-necked flask (40), a heating mantle (30), a condenser (50), a cylindrical funnel (80), and a gas washing bottle (10). The heating mantle (30) is used to heat or cool the two-necked flask (40) by placing the two-necked flask (40) inside the heating mantle (30). The condenser (50) has one end connected to one opening of the two-necked flask (40) and the other end connected to one end of the cylindrical funnel (80) through a flexible tube (51). The cylindrical funnel (80) is connected to the gas washing bottle. A gas washing bottle (10) is provided with a glass tube (11) inside the gas washing bottle (10). One end of the glass tube (11) extends into the interior of the washing bottle (10), and the other end of the glass tube (11) extends to the outside of the washing bottle (10) and is connected to the cylindrical funnel (80) through a flexible tube (52).

2. The xanthan gum content decarboxylation method detection device according to claim 1, characterized in that: The condenser tube (50) is equipped with a cooling water source interface (70) for connecting and circulating cooling water.

3. The xanthan gum content decarboxylation method detection device according to claim 1, characterized in that: It also includes a gas supply device (60).

4. The xanthan gum content decarboxylation method detection device according to claim 1, characterized in that: The gas supply device (60) mainly includes a nitrogen cylinder (61), a flow meter (62), a first delivery pipe (63), and a second delivery pipe (64).

5. The xanthan gum content decarboxylation method detection device according to claim 4, characterized in that: The flow meter (62) is installed on the first delivery pipe (63); one end of the first delivery pipe (63) is connected to the outlet of the nitrogen cylinder (61), and the other end is connected to the inlet of the flow meter (62); the second delivery pipe (64) is connected to the outlet of the flow meter (62) at one end, and the other end is connected to the two-necked flask (40).

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