An antibody elisa test kit integrated with microneedle array

Abstract

The utility model belongs to ELISA detection kit technical field provides an antibody ELISA detection kit of integrated microneedle array, include: detection board and guide plate, are used for guide plate cooperation detection board to complete reagent detection operation, detection mechanism sets up on the detection board is used for reagent mixing detection, storage mechanism sets up above the detection board is used for reagent storage, waste liquid mechanism sets up below the detection board is used for waste liquid collection, pressurizing mechanism sets up on guide plate is used for to reagent auxiliary pressurization and mixes operation, by several pressurizing pipes cooperation guide plate and air pump to several reagent cylinder and microneedle array cylinder carry out synchronous pressurization, and by several microneedle array cylinder bottom's microneedle array cooperation adds mixing to increase antibody's coating area, improve detection sensitivity, to this realizes the purpose of multiple detection reagent synchronous mixing detection, and then improves reagent detection operation efficiency.

Description

Technical Field

[0001] This invention belongs to the technical field of ELISA detection kits, specifically an antibody ELISA detection kit with an integrated microneedle array. Background Technology

[0002] ELISA test kits are biological detection tools developed based on enzyme-linked immunosorbent assay (ELISA) technology. They are used to quantitatively or qualitatively detect the content of specific antigens or antibodies in samples and are widely used in medical diagnostics, biomedical research, food safety testing, environmental monitoring and other fields. ELISA technology utilizes the specific binding reaction between antigens and antibodies, combined with the amplification effect of enzyme-catalyzed reactions, to achieve sensitive detection of target substances.

[0003] However, during the testing process, each reagent needs to be added individually, resulting in overly complicated procedures and low efficiency.

[0004] To address these issues, those skilled in the art have proposed an antibody ELISA detection kit with an integrated microneedle array to resolve the problems raised in the background. Utility Model Content

[0005] To address the aforementioned technical problems, this invention provides an antibody ELISA detection kit with an integrated microneedle array, thereby solving the problem of low reagent detection efficiency in the prior art.

[0006] An antibody ELISA test kit with an integrated microneedle array includes: a test plate and an air guide plate for the air guide plate to cooperate with the test plate to complete the reagent detection operation; a detection mechanism disposed on the test plate for reagent mixing and detection; a storage mechanism disposed above the test plate for reagent storage; a waste liquid mechanism disposed below the test plate for waste liquid collection; and a pressurization mechanism disposed on the air guide plate for assisting in pressurizing the reagents for mixing.

[0007] Preferably, the detection mechanism includes a plurality of microneedle array tubes fixedly disposed on the detection plate, the bottom of the microneedle array tubes having a microneedle array, and the top of the detection plate having a plurality of square grooves; a sealing ring is fixedly disposed at the bottom of the detection plate, and limit shafts are fixedly disposed at the four corners of the bottom of the detection plate.

[0008] Preferably, the storage mechanism includes a storage plate that is engaged with the top of the detection plate, the storage plate having a plurality of storage slots, and a plurality of reagent cylinders placed on the storage plate through the plurality of storage slots; two limiting clamps are symmetrically and slidably arranged on the inner wall of the storage slots, and a plurality of springs are fixedly connected to the side wall of the limiting clamps.

[0009] Preferably, the storage mechanism further includes a receiving sleeve fixedly disposed on the top of the reagent cylinder, and a sealing cap is elastically rotatably disposed at the bottom of the reagent cylinder; a torque shaft is rotatably disposed between the reagent cylinder and the sealing cap, a sealing ring is fixedly disposed on the inner wall of the sealing cap, and magnetic blocks are fixedly disposed on both the bottom of the reagent cylinder and the inner wall of the sealing cap.

[0010] Preferably, the waste liquid mechanism includes a waste liquid plate that is engaged with the bottom of the detection plate, and a plurality of waste liquid chambers are fixedly disposed inside the waste liquid plate; limit holes are provided at the four corners of the top of the waste liquid plate.

[0011] Preferably, the pressurizing mechanism includes a plurality of threaded holes formed at the bottom of the air guide plate, and a plurality of threaded tubes are screwed onto the air guide plate through the plurality of threaded holes. A pressurizing tube is fixedly disposed at the bottom of the threaded tubes; and an air pump is fixedly disposed on the side wall of the air guide plate.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] This invention features a storage plate that can hold multiple reagent cartridges, facilitating the loading of both reagents to be added and reagents to be tested. A detection plate is snapped into the bottom of the storage plate, and multiple microneedle array cartridges are fixedly connected to the detection plate. Reagent cartridges containing reagents to be added are snapped into the top of the microneedle array cartridges, while reagent cartridges containing reagents to be tested are snapped into the bottom. Multiple pressurization tubes, along with an air guide plate and an air pump, synchronously pressurize the reagent cartridges and microneedle array cartridges. The microneedles at the bottom of the microneedle array cartridges also contribute to the mixing and addition of reagents, thereby increasing the antibody coating area and improving detection sensitivity. This achieves the goal of simultaneous mixing and detection of multiple reagents, thus improving the efficiency of reagent testing operations. Attached Figure Description

[0014] Figure 1 This is a structural diagram of the ELISA detection kit of this utility model;

[0015] Figure 2 This utility model Figure 1 Structural diagram at point A;

[0016] Figure 3 This is a structural diagram showing the distribution of the storage plate, detection plate, and waste liquid plate of this utility model.

[0017] Figure 4 This is a structural diagram of the air guide plate connection of this utility model;

[0018] Figure 5 This is a diagram of the pressure pipe connection structure of this utility model;

[0019] Figure 6This is a diagram showing the connection structure of the reagent cylinder of this utility model;

[0020] Figure 7 This is a diagram of the connection structure of the limiting clamping block of this utility model;

[0021] Figure 8 This is a schematic diagram of the connection structure of the microneedle array tube of this utility model.

[0022] In the picture:

[0023] 1. Storage plate; 2. Detection plate; 3. Waste liquid plate; 4. Waste liquid chamber; 5. Limiting hole; 6. Microneedle array cylinder; 7. Reagent cylinder; 8. Gas guide plate; 9. Air pump; 10. Storage tank; 11. Limiting clamp; 12. Limiting shaft; 13. Sealing ring one; 14. Threaded hole; 15. Pressurization tube; 16. Threaded tube; 17. Receiving sleeve; 18. Sealing cap; 19. Magnetic block; 20. Torque shaft; 21. Sealing ring two; 22. Spring. Detailed Implementation

[0024] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0025] Example 1:

[0026] As attached Figure 1 To be continued Figure 8 As shown:

[0027] This invention provides an antibody ELISA detection kit with an integrated microneedle array, comprising: a detection plate 2 and an air guide plate 8, for the air guide plate 8 to cooperate with the detection plate 2 to complete the reagent detection operation; a detection mechanism disposed on the detection plate 2 for reagent mixing and detection; a storage mechanism disposed above the detection plate 2 for reagent storage; a waste liquid mechanism disposed below the detection plate 2 for waste liquid collection; and a pressurization mechanism disposed on the air guide plate 8 for assisting in pressurizing the reagents for mixing.

[0028] By incorporating a storage plate 1, several reagent cartridges 7 are loaded, facilitating the loading of reagent cartridges 7 containing reagents to be added and reagent cartridges 7 containing reagents to be tested. A detection plate 2 is snapped into the bottom of the storage plate 1, and several microneedle array cartridges 6 are fixedly connected to the detection plate 2. The reagent cartridges 7 containing reagents to be added are snapped into the top of the microneedle array cartridges 6, and the reagent cartridges 7 containing reagents to be tested are snapped into the bottom of the microneedle array cartridges 6. Several pressurization tubes 15, together with an air guide plate 8 and an air pump 9, synchronously pressurize the reagent cartridges 7 and microneedle array cartridges 6. The microneedles at the bottom of the microneedle array cartridges 6 are used to add and mix the reagents, thereby increasing the antibody coating area and improving the detection sensitivity. This achieves the purpose of synchronous mixing and detection of multiple test reagents, thereby improving the efficiency of reagent detection operations.

[0029] refer to Figure 1 , Figure 3 and Figure 8 The testing mechanism includes a testing plate 2 and a microneedle array tube 6;

[0030] Several microneedle array tubes 6 are fixedly connected to the detection plate 2, and microneedle arrays are formed at the bottom of each microneedle array tube 6. The microneedles have a diameter of 100 micrometers and a height of 100 micrometers. The microneedle array at the bottom of the microneedle array tube 6 consists of more than 4,100 microneedles. This allows for reagent conduction inside the microneedle array tube 6 during the detection process. When the reagents are mixed, the microneedle array structure at the bottom of the microneedle array tube 6 can increase the coating area of ​​the antibody and improve the detection sensitivity.

[0031] Meanwhile, several square slots are provided on the top of the detection plate 2, so that the reagent cylinder 7 can be placed in the detection plate 2 through the square slots and engaged in the microneedle array cylinder 6, so that the reagent in the reagent cylinder 7 can be introduced into the interior of the microneedle array cylinder 6. A sealing ring 13 is fixedly connected to the bottom of the detection plate 2, so that when the detection plate 2 and the waste liquid plate 3 are engaged, the sealing ring 13 can seal and protect between the detection plate 2 and the waste liquid plate 3. Limiting shafts 12 are fixedly connected at the four corners of the bottom of the detection plate 2, so that the detection plate 2 can be engaged and connected to the waste liquid plate 3 through several limiting shafts 12, thereby facilitating the use of reagent mixing detection when the detection plate 2 is engaged and connected to the waste liquid plate 3.

[0032] refer to Figure 1 , Figure 2 , Figure 6 and Figure 7 The storage mechanism includes a storage plate 1, a storage tank 10, a reagent cylinder 7, and a sealing cap 18;

[0033] A storage plate 1 is snapped onto the top of the detection plate 2, and several storage slots 10 are provided on the storage plate 1. This allows the storage plate 1 to hold several reagent cylinders 7 through the storage slots 10. Two limiting clamps 11 are elastically slidably connected to the side wall of the storage slots 10 by several springs 22. This allows the two limiting clamps 11 to limit and clamp the reagent cylinders 7 when they are placed inside the storage slots 10, thereby achieving the purpose of storing the reagent cylinders 7 stably in the storage plate 1, which facilitates the stable transport and use of the reagent cylinders 7 loaded with medicine.

[0034] Meanwhile, a receiving sleeve 17 is fixedly connected to the top of the reagent cylinder 7, so that during use, the reagent cylinder 7 can be screwed onto a protective cap through the receiving sleeve 17 to protect the reagent placed inside the reagent cylinder 7. A sealing cap 18 is elastically rotatably connected to the bottom of the reagent cylinder 7 through a torque shaft 20. A sealing ring 21 is fixedly connected to the inner wall of the sealing cap 18, so that the sealing cap 18 can protect the bottom of the reagent cylinder 7. The sealing ring 21 can seal the bottom of the reagent cylinder 7, thereby preventing the reagent inside the reagent cylinder 7 from leaking directly from the bottom of the reagent cylinder 7. Magnetic blocks 19 are fixedly connected to the bottom of the reagent cylinder 7 and the inner wall of the sealing cap 18, so that when the sealing cap 18 is elastically engaged with the bottom of the reagent cylinder 7 through the torque shaft 20, the two magnetic blocks 19 can attract each other, so that the sealing cap 18 can work with the torque shaft 20 to tightly protect the bottom of the reagent cylinder 7.

[0035] refer to Figure 1 , Figure 3 and Figure 8 The waste liquid mechanism includes a waste liquid plate 3, a waste liquid chamber 4, and a limiting hole 5;

[0036] By engaging a waste liquid plate 3 at the bottom of the detection plate 2 and fixing a waste liquid chamber 4 to the waste liquid plate 3, the waste reagent liquid mixed in the microneedle array cylinder 6 and reagent cylinder 7 can be collected inside the waste liquid chamber 4, thus preventing waste leakage and contamination. During the reagent mixing and testing process, a reagent cylinder 7 containing the reagent to be tested is first engaged with the bottom of the microneedle array cylinder 6 through the receiving sleeve 17 at its top, so that the microneedle array at the bottom of the microneedle array cylinder 6 extends into the reagent cylinder at its bottom. Inside the reagent tube 7, the reagent tube 7 is brought into contact with the reagent to be tested. Then, the reagent tube 7 containing the reagent to be added is snapped onto the top of the microneedle array tube 6. This applies pressure to the reagent tube 7 at the top of the microneedle array tube 6, causing the reagent to be added in the reagent tube 7 at the top of the microneedle array tube 6 to be introduced from the microneedle array at the bottom of the microneedle array tube 6 into the reagent tube 7 at the bottom of the microneedle array tube 6, where it is mixed with the reagent to be tested for detection. The microneedle array structure can increase the coating area of ​​the antibody in the reagent to be added and the reagent to be tested, thereby improving the detection sensitivity.

[0037] Meanwhile, limiting holes 5 are provided at the four corners of the top of the waste liquid plate 3, so that when the detection plate 2 is engaged with the waste liquid plate 3, the limiting holes 5 can cooperate with several limiting shafts 12 at the four corners of the bottom of the detection plate 2, so that the detection plate 2 and the waste liquid plate 3 are tightly engaged through several limiting shafts 12 and limiting holes 5, and the sealing ring 13 at the bottom of the detection plate 2 is engaged in the groove at the top of the waste liquid plate 3, so that the detection plate 2 and the waste liquid plate 3 can be sealed and protected, thereby preventing waste liquid from leaking out from the connection between the detection plate 2 and the waste liquid plate 3.

[0038] Example 2:

[0039] refer to Figure 1 , Figure 4 , Figure 5 and Figure 8 The pressurization mechanism includes an air guide plate 8, an air pump 9, and a pressurization pipe 15;

[0040] By setting an air guide plate 8 and opening several threaded holes 14 at the bottom of the air guide plate 8, the interior of the air guide plate 8 is a hollow structure and connected to the several threaded holes 14. An air pump 9 is fixedly connected to the side wall of the air guide plate 8, so that the air pump 9 can pressurize the interior of the air guide plate 8 and then evenly discharge it through the several threaded holes 14. The pressurization pressure of the air pump 9 can be adjusted according to the actual use requirements.

[0041] Meanwhile, a threaded tube 16 is screwed into the bottom of the gas guide plate 8 through several threaded holes 14, and a pressure tube 15 is fixedly connected to the bottom of the threaded tube 16. This allows the gas guide plate 8 to be used in the following ways: first, several reagent cylinders 7 containing reagents to be tested are snapped into the bottom of several microneedle array cylinders 6; then, several reagent cylinders 7 containing reagents to be added are snapped into the top of several microneedle array cylinders 6, with the detection plate 2 and waste liquid plate 3 locked together. The storage plate 1 is placed separately on the side. Finally, several pressure tubes 15 are snapped into the reagent cylinders 7 containing reagents to be added. In other words, several pressure tubes 15 and the gas guide plate 8 are snapped into the top of several microneedle array cylinders 6 and reagent cylinders 7. This allows the air pump 9 to pressurize several reagent cylinders 7 containing reagents to be added through the air guide plate 8 and several pressurization tubes 15, causing the sealing caps 18 at the bottom of several reagent cylinders 7 to open simultaneously, and the reagents to be added to be introduced into the interior of the microneedle array cylinder 6. At this time, the pressurization force of the air pump 9 is increased, and the reagents to be added are then guided to the reagents to be tested in several reagent cylinders 7 simultaneously through the microneedle array at the bottom of several microneedle array cylinders 6 for mixing. In this way, the reagents to be added in several reagent cylinders 7 are guided by the microneedle array at the bottom of several microneedle array cylinders 6 to the reagents to be tested in several reagent cylinders 7, thereby improving the operating efficiency.

[0042] Meanwhile, after the reagent mixing and detection are completed, the gas guide plate 8 and several pressurizing tubes 15 are pulled out from several reagent cylinders 7 containing reagents to be added, and several microneedle array cylinders 6 and reagent cylinders 7 containing reagents to be added are pulled out from reagent cylinders 7 containing reagents to be tested. Then, several pressurizing tubes 15 and gas guide plate 8 are locked onto several reagent cylinders 7 containing reagents to be tested, and pressurization is applied to them, so that the mixed reagents in the reagent cylinders 7 containing reagents to be tested leak out from the sealing cap 18 at the bottom of the reagent cylinder 7 and are introduced into the waste liquid chamber 4 for collection and treatment.

[0043] Secondly, the pressure of the air pump 9 can be adjusted according to actual usage needs, and can pressurize the reagent cylinder 7 containing the reagent to be added, so that the sealing cap 18 at the bottom of the cylinder can be opened without affecting the sealing cap 18 at the bottom of the reagent cylinder 7 containing the reagent to be tested.

[0044] Working principle: Several microneedle array cylinders 6 are fixedly connected to the detection plate 2, and microneedle arrays are formed at the bottom of each microneedle array cylinder 6. The microneedles are 100 micrometers in diameter and 100 micrometers in height. The microneedle array at the bottom of the microneedle array cylinder 6 consists of more than 4100 microneedles. This allows for reagent conduction within the microneedle array cylinder 6 during the detection process. When the reagents are mixed, the microneedle array structure at the bottom of the microneedle array cylinder 6 increases the antibody coating area and improves the detection sensitivity. During use, the gas guide plate 8 is used by first attaching several reagent cylinders 7 containing the reagents to be tested to the bottom of several microneedle array cylinders 6, then attaching several reagent cylinders 7 containing the reagents to be added to the top of several microneedle array cylinders 6, and locking the detection plate 2 and waste liquid plate 3 together. The storage plate 1 is placed separately on the side. Finally, several gas guide plates 8 are attached to the bottom of the microneedle array cylinders 6. The pressure tube 15 is snapped into several reagent cylinders 7 containing reagents to be added. That is, several pressure tubes 15 and air guide plates 8 are snapped into the top of several microneedle array cylinders 6 and reagent cylinders 7, so that the air pump 9 can pressurize the several reagent cylinders 7 containing reagents to be added through the air guide plates 8 and several pressure tubes 15, causing the sealing caps 18 at the bottom of several reagent cylinders 7 to open simultaneously, and the reagents to be added are synchronously introduced into the interior of the microneedle array cylinders 6. At this time, the pressure of the air pump 9 is increased, and the reagents to be added are synchronously guided to the reagents to be tested in several reagent cylinders 7 through the microneedle array at the bottom of several microneedle array cylinders 6 for mixing. In this way, the reagents to be added in several reagent cylinders 7 are synchronously guided by the microneedle array at the bottom of several microneedle array cylinders 6 to the reagents to be tested in several reagent cylinders 7, thereby improving the operating efficiency.

[0045] The embodiments of this utility model are given for the purpose of illustration and description. Although embodiments of this utility model have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the utility model. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this utility model.

[0046] Finally, the following points should be noted: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installation", "connection", and "linkage" should be interpreted broadly, and can be mechanical or electrical connections, or internal connections between two components, or direct connections. "Up", "down", "left", "right", etc. are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may change.

[0047] Secondly: The accompanying drawings of the embodiments disclosed in this utility model only involve the structures involved in the embodiments disclosed in this utility model. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of this utility model can be combined with each other.

[0048] Finally: The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

[0049] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. An integrated microneedle array antibody ELISA test kit, characterized by, include: The detection plate (2) and the gas guide plate (8) are used to cooperate with the detection plate (2) to complete the reagent detection operation; The detection mechanism is set on the detection plate (2) and is used for reagent mixing detection. The detection mechanism includes several microneedle array tubes (6) fixedly set on the detection plate (2). The microneedle array tubes (6) have microneedle arrays at the bottom and several square grooves at the top of the detection plate (2). A sealing ring (13) is fixedly set at the bottom of the detection plate (2) and a limit shaft (12) is fixedly set at each of the four corners of the bottom of the detection plate (2). A storage mechanism is set above the detection plate (2) for reagent storage; the storage mechanism includes a storage plate (1) that is engaged with the top of the detection plate (2), the storage plate (1) has several storage slots (10) and several reagent cylinders (7) are placed on the storage plate (1) through the several storage slots (10); two limiting clamps (11) are symmetrically slidably arranged on the inner wall of the storage slots (10), and several springs (22) are fixedly connected to the side wall of the limiting clamps (11); Waste liquid mechanism, located below the detection plate (2), is used for waste liquid collection; the waste liquid mechanism includes a waste liquid plate (3) that is engaged with the bottom of the detection plate (2), and several waste liquid chambers (4) are fixedly arranged inside the waste liquid plate (3); limit holes (5) are opened at the four corners of the top of the waste liquid plate (3). A pressurizing mechanism is provided on the air guide plate (8) for mixing reagents by pressurizing them. The pressurizing mechanism includes several threaded holes (14) at the bottom of the air guide plate (8). Several threaded tubes (16) are screwed onto the air guide plate (8) through the several threaded holes (14). A pressurizing tube (15) is fixedly provided at the bottom of the threaded tubes (16). An air pump (9) is fixedly provided on the side wall of the air guide plate (8).

2. The antibody ELISA test kit of integrated microneedle array as claimed in claim 1 wherein: The storage mechanism also includes a receiving sleeve (17) fixedly installed on the top of the reagent cylinder (7), and a sealing cap (18) is elastically rotatably installed at the bottom of the reagent cylinder (7). A torque shaft (20) is rotatably provided between the reagent cylinder (7) and the sealing cap (18). A sealing ring (21) is fixedly provided on the inner wall of the sealing cap (18). Magnetic blocks (19) are fixedly provided at the bottom of the reagent cylinder (7) and on the inner wall of the sealing cap (18).

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