A tobacco bacterial root disease inoculation device

Abstract

The utility model belongs to tobacco planting technical field, concretely relates to a kind of tobacco bacterial root disease inoculation device. Specifically related to a kind of tobacco bacterial root disease inoculation device, including support, at least one set of injection mechanism and injection mechanism fixing frame;The injection mechanism fixing frame is fixedly connected with the support, the injection mechanism is assembled on the injection mechanism fixing frame, for injecting pathogenic bacteria to tobacco root to complete inoculation operation, the present tobacco bacterial root disease inoculation device moves along vertical guide rail by driving component and drives injector, replaces traditional manual pressing operation, can accurately control injection stroke, ensure that injector can reach tobacco root preset position directly each time inoculation, effectively avoid the problem that needle is inserted too shallow or too deep due to manual operation.

Description

Technical Field

[0001] This utility model belongs to the field of tobacco planting technology, specifically relating to an inoculation device for bacterial root diseases in tobacco. Background Technology

[0002] In tobacco pathology research, core research directions such as assessing the pathogenicity of tobacco pathogens and elucidating the interaction mechanisms between pathogens and tobacco plants all rely on precise and efficient disease inoculation experiments. The scientific nature of the inoculation method and the reliability of the inoculation device directly determine the accuracy of experimental data and research efficiency, and are an important foundation for the validity of subsequent research conclusions. Currently, injection-type inoculation methods such as needle pricking are commonly used in plant disease inoculation experiments. For the inoculation needs of bacterial root diseases in tobacco, relevant specialized devices have been disclosed in the existing technology (such as Chinese utility model patent, application number: 202322389921.9). The core design idea is to deliver bacterial solutions containing pathogens to the tobacco roots through injection to simulate the natural infection process.

[0003] However, existing tobacco bacterial root disease inoculation devices have significant shortcomings in practical applications, making it difficult to meet the needs of efficient and precise laboratory research. Specific deficiencies are as follows:

[0004] The injection stroke is unstable and the inoculation accuracy is low: The existing device uses a manual pressing method to drive the syringe for injection. Due to the difference in the pressure and hand movement of the operator, the vertical stroke of each injection cannot be kept consistent. As a result, the syringe needle often fails to accurately reach the preset inoculation position on the tobacco root. In some cases, the needle is inserted too shallowly (not reaching the core area of ​​the root) or too deeply (damaging the tobacco root system). Both of these will lead to inconsistent pathogen infection conditions, which in turn will cause experimental data deviation and affect the reliability of research results such as pathogenicity assessment and interaction mechanism analysis.

[0005] In laboratory research, it is often necessary to conduct parallel inoculation experiments on multiple tobacco samples. However, the manual pressing operation mode of the existing device requires the operator to perform pressing and resetting actions on each plant. The operation process is cumbersome, time-consuming and labor-intensive, which cannot meet the needs of efficient inoculation of batch samples and significantly restricts the experimental progress. Utility Model Content

[0006] The purpose of this invention is to provide an inoculation device for tobacco bacterial root diseases to solve the problems existing in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A device for inoculating tobacco bacterial root diseases includes a support, at least one set of injection mechanisms, and an injection mechanism fixing frame; the injection mechanism fixing frame is fixedly connected to the support, and the injection mechanism is assembled on the injection mechanism fixing frame for injecting pathogens into tobacco roots to complete the inoculation operation;

[0009] The injection mechanism includes a drive component, a vertical guide rail, a syringe, and a syringe guide block. The drive component is detachably connected to the injection mechanism mounting frame via a support frame. The vertical guide rail is fixed to the side wall of the injection mechanism mounting frame in a vertical direction. One side of the syringe guide block is slidably engaged with the vertical guide rail via a slider, and the other side is used to mount the syringe. The top of the syringe guide block is fixedly connected to the movable end of the drive component. The syringe inlet is connected to an external liquid storage tank via a hose.

[0010] Preferably, it also includes a cleaning mechanism for cleaning soil from the outer wall of the syringe, the cleaning mechanism being disposed on the front side of the syringe and fixedly connected to the bottom of the injection mechanism fixing frame;

[0011] The cleaning mechanism consists of a transverse guide rail, a guide block, a left clamping block, a right clamping block, a left clamping plate, a right clamping plate, and a tension spring. The transverse guide rail is fixed horizontally at the bottom edge of the injection mechanism's mounting frame. The left and right clamping blocks are symmetrically arranged at both ends of the transverse guide rail and are slidably connected to it. The left clamping plate is vertically fixed to the bottom end face of the left clamping block, and the right clamping plate is vertically fixed to the bottom end face of the right clamping block. The tension spring is horizontally tensioned between the left and right clamping blocks, and the central axis of the tension spring is aligned with the extension direction of the transverse guide rail.

[0012] Preferably, the left clamping plate has an arc-shaped groove on one side wall near the right clamping plate, and the right clamping plate has a corresponding arc-shaped groove of the same specification on one side wall near the left clamping plate; when the tension spring is in a naturally tensioned state, the left clamping plate and the right clamping plate are interlocked, and the arc-shaped grooves on both sides together form a circular clamping hole that fits the outer wall of the syringe, and the inner wall of the circular clamping hole is in close contact with the outer wall of the syringe.

[0013] Preferably, the guide block has an inverted trapezoidal structure, with its top fixedly connected to the bottom end face of the syringe guide block, and the center line of the guide block is collinear with the center line of the syringe; the inclined surfaces on the left and right sides of the guide block are chamfered, and the left clamping block near the guide block and the right clamping block near the guide block are both provided with inclined surfaces that are adapted to the chamfered inclined surfaces of the guide block. When the guide block moves downward in the vertical direction, its chamfered inclined surfaces can fit with the inclined surfaces of the left and right clamping blocks and push them to slide in the opposite direction along the transverse guide rail.

[0014] Preferably, the injection mechanism fixing frame includes a front upright plate, a rear upright plate, and at least two connecting columns; the front upright plate and the rear upright plate are parallel and spaced apart, the connecting columns are distributed horizontally between the front upright plate and the rear upright plate, and one end of the connecting column is fixedly connected to the rear wall of the front upright plate and the other end is fixedly connected to the front wall of the rear upright plate, and the front upright plate and the rear upright plate form a frame structure through the connecting columns.

[0015] Preferably, the injection mechanism is provided in two sets, one set of vertical guide rails is fixed to the front wall of the front upright plate, and the other set of vertical guide rails is fixed to the rear wall of the rear upright plate. The two sets of injection mechanisms are symmetrically distributed in the front-rear direction, and the center lines of the syringes of the two sets of injection mechanisms are in the same vertical plane.

[0016] The beneficial effects of this invention are as follows: This tobacco bacterial root disease inoculation device uses a drive component to move the syringe along a vertical guide rail, replacing the traditional manual pressing operation. It can precisely control the injection stroke, ensuring that the syringe reaches the preset position on the tobacco root each time, effectively avoiding the problem of needle insertion that is too shallow or too deep due to manual operation. This ensures the consistency of pathogen infection conditions, significantly improves inoculation accuracy, and provides more reliable experimental data support for research on the pathogenicity assessment of tobacco pathogens and pathogen-plant interactions. At the same time, the automated operation mode driven by the drive component greatly simplifies the inoculation process, reduces manual intervention, and is especially suitable for batch inoculation scenarios of multiple tobacco samples, significantly reducing the labor intensity of operators and improving experimental efficiency.

[0017] In addition, the device is equipped with a cleaning mechanism that can automatically clean the soil adhering to the outer wall of the syringe during the inoculation process: when the syringe moves upward, the left and right clamps are engaged by the tension spring, and the soil is scraped off by the arc groove against the outer wall of the syringe. When the syringe moves downward, the guide block can automatically push the clamps open without affecting the inoculation. This effectively prevents the syringe from becoming clogged due to long-term soil adsorption and hardening, reduces the frequency and cost of device maintenance, extends the service life of the syringe, ensures the continuous and stable inoculation operation, and further improves the practicality and ease of use of the device. Attached Figure Description

[0018] Figure 1 This is a perspective view of the present invention;

[0019] Figure 2 This is a partial enlarged view of the present invention. Detailed Implementation

[0020] The specific embodiments of this utility model are described in detail below with reference to the accompanying drawings and preferred embodiments.

[0021] like Figure 1As shown, an inoculation device for tobacco bacterial root diseases includes a support 1, at least one set of injection mechanisms 2, and an injection mechanism fixing frame 3.

[0022] The injection mechanism is fixed to the support and is used for inoculating the tobacco roots. The injection mechanism 2 includes a drive component 21, a vertical guide rail 22, a syringe 23, and a syringe guide block 24. The drive component 21 is mounted on the injection mechanism mounting frame via a support frame. The vertical guide rail is fixed to the injection mechanism mounting frame. The syringe guide block is slidably connected to the vertical guide rail via a slider. The upper part of the syringe guide block is connected to the movable end of the drive component. The syringe is mounted on the syringe guide block and is connected to a storage tank via a hose (this part is a conventional structure). The drive component moves the syringe downwards with a fixed stroke, allowing it to reach the tobacco roots directly.

[0023] Furthermore, since some soil adheres to the syringe each time it is withdrawn, it will accumulate around the syringe over time. If the syringe is not used for a long time, it will dry and harden, clogging the syringe. Therefore, this device has a cleaning mechanism 4 on one side of the syringe.

[0024] The cleaning mechanism includes a transverse guide rail 41, a guide block 42, a left clamping block 43, a right clamping block 44, a left clamping plate 45, a right clamping plate 46, and a tension spring 47. The transverse guide rail is fixed to the bottom of the injection mechanism fixing frame. The left and right clamping blocks are slidably connected to the transverse guide rail, and the left and right clamping plates are fixed to the bottom of the left and right clamping blocks, respectively. The two ends of the tension spring are connected to the left and right clamping blocks, respectively. Furthermore, the left and right clamping plates are respectively provided with grooves. When the left and right clamping plates are fastened, they will fasten onto the syringe from both sides. As the syringe moves upward, the dirt on the syringe is scraped off. The guide block 42 is fixed on the syringe guide block 24 and has chamfers on both sides. The left and right clamping blocks also have chamfers. When the guide block 42 moves downward, it separates the left and right clamping blocks, thereby causing the left and right clamping plates to separate.

[0025] Furthermore, the injection mechanism fixing frame 3 includes a front upright plate 31, a rear upright plate 32, and a connecting column 33. The front upright plate and the rear upright plate are connected together by the connecting column, separating the two injection mechanisms.

[0026] When using the syringe, first place the planting pot containing the tobacco seedlings directly below the syringe. According to the depth of the tobacco roots, set the downward stroke of the drive component (electric push rod) through the controller. Open the valve between the reservoir and the syringe to allow the pathogenic bacterial solution to flow into the syringe along the silicone tube. After expelling the air from the syringe, close the valve to complete the equipment debugging and bacterial solution preparation before inoculation.

[0027] The drive unit is activated, and its movable end extends downward, driving the syringe guide block to move vertically downward along the vertical guide rail. During the downward movement, the guide block at the bottom of the syringe guide block moves downward synchronously, and the chamfered bevels on both sides of the guide block fit into the inclined surfaces of the left and right clamping blocks. As the guide block continues to move downward, it pushes the left and right clamping blocks to slide in the opposite direction along the horizontal guide rail, thereby causing the left and right clamping plates to separate and avoid obstructing the downward movement of the syringe. When the drive unit reaches the preset stroke, the syringe needle is precisely inserted into the preset position at the root of the tobacco. At this time, the valve on the silicone tubing is opened, and the bacterial solution in the reservoir flows into the syringe under gravity or slight pressure. Then, the syringe plunger is pushed to inject the bacterial solution into the root of the tobacco, completing a single inoculation.

[0028] After inoculation, the moving end of the drive component retracts upward, causing the syringe guide block and syringe to move upward along the vertical guide rail. At this time, the guide block moves upward and gradually disengages from the left and right clamps. Under the tension of the tension spring, the left and right clamps slide towards each other along the horizontal guide rail, causing the left and right clamps to re-engage. After engagement, the arc-shaped grooves of the left and right clamps fit against the outer wall of the syringe. As the syringe continues to move upward, the rubber pad on the inner wall of the arc-shaped groove scrapes off the soil adhering to the outer wall of the syringe, achieving automatic cleaning of the syringe. When the syringe is completely retracted to the initial position, the drive component and valve are closed, and the inoculation operation of the next tobacco seedling can be carried out. If batch inoculation is required, simply move the planting pots under the syringe in sequence and repeat the above inoculation process.

[0029] It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model.

Claims

1. A device for inoculating tobacco bacterial root diseases, characterized in that, It includes a support (1), at least one set of injection mechanisms (2) and an injection mechanism fixing frame (3); the injection mechanism fixing frame (3) is fixedly connected to the support (1), and the injection mechanism (2) is assembled on the injection mechanism fixing frame (3) for injecting pathogens into the roots of tobacco to complete the inoculation operation; The injection mechanism (2) includes a drive component (21), a vertical guide rail (22), a syringe (23), and a syringe guide block (24). The drive component (21) is detachably connected to the injection mechanism fixing frame (3) via a support frame. The vertical guide rail (22) is fixed vertically to the side wall of the injection mechanism fixing frame (3). One side of the syringe guide block (24) is slidably engaged with the vertical guide rail (22) via a slider, and the other side is used to install the syringe (23). The top of the syringe guide block (24) is fixedly connected to the movable end of the drive component (21). The inlet of the syringe (23) is connected to an external liquid storage tank via a hose.

2. The tobacco bacterial root disease inoculation device according to claim 1, characterized in that, It also includes a cleaning mechanism (4) for cleaning the soil on the outer wall of the syringe (23), the cleaning mechanism (4) being disposed on the front side of the syringe (23) and fixedly connected to the bottom of the injection mechanism fixing frame (3); The cleaning mechanism (4) consists of a transverse guide rail (41), a guide block (42), a left clamping block (43), a right clamping block (44), a left clamping plate (45), a right clamping plate (46), and a tension spring (47). The transverse guide rail (41) is fixed horizontally at the bottom edge of the injection mechanism fixing frame (3). The left clamping block (43) and the right clamping block (44) are symmetrically arranged at both ends of the transverse guide rail (41) and are slidably connected to the transverse guide rail (41). The left clamping plate (45) is vertically fixed to the bottom end face of the left clamping block (43), and the right clamping plate (46) is vertically fixed to the bottom end face of the right clamping block (44). The tension spring (47) is horizontally tensioned between the left clamping block (43) and the right clamping block (44), and the central axis of the tension spring (47) is consistent with the extension direction of the transverse guide rail (41).

3. The tobacco bacterial root disease inoculation device according to claim 2, characterized in that, The left clamp (45) has an arc-shaped groove on one side wall near the right clamp (46), and the right clamp (46) has an arc-shaped groove of the same specification on one side wall near the left clamp (45). When the tension spring (47) is in a naturally tensioned state, the left clamp (45) and the right clamp (46) are interlocked, and the arc-shaped grooves on both sides together form a circular clamping hole that fits the outer wall of the syringe (23), and the inner wall of the circular clamping hole is in close contact with the outer wall of the syringe (23).

4. The tobacco bacterial root disease inoculation device according to claim 2, characterized in that, The guide block (42) has an inverted trapezoidal structure. Its top is fixedly connected to the bottom end face of the syringe guide block (24), and the center line of the guide block (42) is collinear with the center line of the syringe (23). The inclined surfaces on the left and right sides of the guide block (42) are chamfered. The left clamping block (43) near the guide block (42) and the right clamping block (44) near the guide block (42) are both provided with inclined surfaces that are adapted to the chamfered inclined surfaces of the guide block (42). When the guide block (42) moves downward in the vertical direction, its chamfered inclined surfaces can fit with the inclined surfaces of the left clamping block (43) and the right clamping block (44) and push them to slide in the opposite direction along the transverse guide rail (41).

5. The tobacco bacterial root disease inoculation device according to claim 1, characterized in that, The injection mechanism fixing frame (3) includes a front upright plate (31), a rear upright plate (32), and at least two connecting columns (33); the front upright plate (31) and the rear upright plate (32) are parallel and spaced apart, and the connecting columns (33) are distributed horizontally between the front upright plate (31) and the rear upright plate (32). One end of the connecting column (33) is fixedly connected to the rear wall of the front upright plate (31), and the other end is fixedly connected to the front wall of the rear upright plate (32). The front upright plate (31) and the rear upright plate (32) form a frame structure through the connecting columns (33).

6. The tobacco bacterial root disease inoculation device according to claim 5, characterized in that, The injection mechanism (2) is provided in two sets. The vertical guide rail (22) of one set of injection mechanism (2) is fixed to the front wall of the front plate (31), and the vertical guide rail (22) of the other set of injection mechanism (2) is fixed to the rear wall of the rear plate (32). The two sets of injection mechanisms (2) are symmetrically distributed in the front-back direction, and the center lines of the syringes (23) of the two sets of injection mechanisms (2) are in the same vertical plane.

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