A test device for studying the effect of root separation in plants

Abstract

The utility model relates to the technical field of agricultural scientific research test facilities discloses a test device of research plant root separation effect. Including culture room, the culture room is equipped with the baffle, the baffle can detachable connection culture room lateral wall, the baffle divides culture room into two independent culture room, the baffle top is equipped with the fixed planting cotton, the fixed planting cotton is fixed with multiple groups of catheter, and each group of catheter is 2 roots, and each culture room is equipped with nutrient solution automatic circulation system and water quality detector, and the nutrient solution automatic circulation system and water quality detector are respectively electric signal connection controller. The utility model catheter inner wall is coated with the hydrophilic nano coating, can guide the capillary root natural shunt, promotes capillary root along the catheter natural extension. Avoid the mechanical damage to the root system (especially capillary root) in the sampling and observation process, guarantee the normal growth of root system and the reliability of subsequent research.

Description

Technical Field

[0001] This utility model relates to the field of agricultural scientific research experimental facilities technology, specifically to an experimental device for studying the effect of root division in plants. Background Technology

[0002] In the fields of agricultural science and plant biology, the growth status of plant roots has a crucial impact on the overall growth and development, stress resistance, yield, and quality of plants. It also has a profound influence on soil and water conservation and resource utilization in the ecological environment. Therefore, research on plant root growth has always been a hot topic in agricultural scientific research. Traditional methods for studying plant roots mainly include digging, soil drilling, and nailing. While these methods have achieved certain research results under specific conditions, they also have drawbacks such as low visualization, significant root damage, inability to monitor in real time, and data lag, especially insufficient protection of capillary roots. Capillary roots are the most active part of the root system and have the highest efficiency in absorbing water and nutrients; however, traditional root research methods are most likely to damage capillary roots. Existing root research methods can only simply observe the influence of the taproot on development, ignoring the role of capillary roots. Given the limitations of traditional research methods, this invention, through optimization of the xylem and automated circulation system, achieves for the first time non-destructive root separation and real-time monitoring of capillary roots, significantly improving experimental accuracy and efficiency. Utility Model Content

[0003] The purpose of this invention is to overcome the shortcomings of the existing technology and provide an experimental device for studying the effect of root division in plants.

[0004] To achieve the above objectives, the technical solution of this utility model is: an experimental device for studying the root division effect of plants, comprising a culture chamber, wherein a partition is provided inside the culture chamber, the partition being detachably connected to the side wall of the culture chamber, the partition dividing the culture chamber into two independent culture rooms, a planting cotton is provided on the top of the partition, and multiple sets of vascular bundles are fixed on the planting cotton, each set consisting of 2 vascular bundles, the 2 vascular bundles in each set being located in the two culture rooms respectively, each culture room being equipped with an automatic nutrient solution circulation system and a water quality detector, the automatic nutrient solution circulation system and the water quality detector being electrically connected to a controller. The automatic nutrient solution circulation system is used to simulate natural growth conditions, and the water quality detector can monitor the nutrient solution status in real time.

[0005] Furthermore, the top of the partition is provided with a slot, and the planted cotton is attached to the slot.

[0006] Furthermore, the automatic nutrient solution circulation system includes an inlet pipe and an outlet pipe installed on the side wall of the culture chamber, a water pump is connected between the inlet pipe and the outlet pipe, the water pump is electrically connected to a controller, and valves are provided on both the inlet pipe and the outlet pipe.

[0007] Furthermore, the inner wall of the catheter is coated with a hydrophilic nano-coating.

[0008] Furthermore, the culture chamber is equipped with a top cover.

[0009] Furthermore, each of the culture chambers is equipped with a root detector on its side wall. The root detector is electrically connected to a computer equipped with a root analysis system and is used to record root growth in real time.

[0010] Furthermore, the side wall of the culture chamber is provided with a connector groove, and the partition is connected to the connector groove.

[0011] Furthermore, both the culture chamber and the partition are made of opaque materials.

[0012] Furthermore, each of the culture chambers is equipped with a drain pipe at the bottom for easy replacement of the nutrient solution, and the drain pipe is equipped with a valve.

[0013] The beneficial effects of this utility model are: (1) Non-destructive monitoring: The inner wall of the vascular bundle is coated with a hydrophilic nano-coating, which can guide the natural diversion of capillary roots and promote the natural extension of capillary roots along the vascular bundle. This avoids mechanical damage to the root system (especially capillary roots) during sampling and observation, ensuring the normal growth of the root system and the reliability of subsequent research.

[0014] (2) Real-time dynamic monitoring: Real-time monitoring of the root system is achieved through the root system detector, capturing subtle changes and key periods in root growth.

[0015] (3) Easy to operate: The automatic nutrient solution circulation system facilitates the simulation of natural growth conditions and improves the accuracy of research. Through the synergistic innovation of the vascular bundle-sensor system-circulation system, the core problem of capillary root research has been solved, and it has the characteristics of high precision, low damage and high efficiency. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the internal structure of the culture chamber.

[0017] In the diagram: 1. Cultivation chamber; 2. Partition; 3. Planted cotton; 4. Water quality detector; 5. Root system detector; 6. Water pump; 7. Top cover; 8. Controller; 9. Guide tube; 10. Water inlet pipe; 11. Water outlet pipe. Detailed Implementation

[0018] Example:

[0019] like Figure 1 and Figure 2As shown, an experimental apparatus for studying the root division effect of plants includes a culture chamber 1. A partition 2 is provided inside the culture chamber 1, and the partition 2 is detachably connected to the side wall of the culture chamber 1, dividing the culture chamber 1 into two independent culture rooms. Both the culture chamber 1 and the partition 2 are made of opaque material. The culture chamber 1 is a rectangular container with a length, width, and height of 20 cm, 20 cm, and 40 cm, respectively. The partition 2 has a length of 19.5 cm and a height of 39.5 cm. A planting cotton 3 is provided on the top of the partition 2, and a slot is provided on the top of the partition 2 for the planting cotton 3 to engage with the slot. The planting cotton 3 is made of sponge material, with a length, width, and height of 14 cm, 4 cm, and 2 cm, respectively. Three sets of vascular bundles 9 are fixed on the planting cotton 3. The vascular bundles 9 have a diameter of 1 cm and a height of 3 cm. The inner wall of the vascular bundles 9 is coated with a hydrophilic nano-coating to guide the natural diversion of capillary roots, promote the natural extension of capillary roots along the vascular bundles 9, and avoid mechanical damage. Each group of tubes 9 consists of 2 tubes, and the 2 tubes 9 in each group are located in two culture rooms. Each culture room is equipped with an automatic nutrient solution circulation system, a water quality detector 4, and a root system detector 5. The automatic nutrient solution circulation system and the water quality detector 4 are electrically connected to the controller 8, and the root system detector 5 is connected to a computer equipped with a root system analysis system.

[0020] The automatic nutrient solution circulation system includes an inlet pipe 10 and an outlet pipe 11 installed on the side wall of the culture chamber 1. A water pump 6 is connected between the inlet pipe 10 and the outlet pipe 11. The water pump 6 is electrically connected to a controller 8. Valves are provided on both the inlet pipe 10 and the outlet pipe 11.

[0021] The culture chamber 1 is equipped with a top cover 7 with a 14 cm × 4 cm opening in the middle. The side walls of the culture chamber 1 are provided with insertion slots, and the partition 2 is inserted into the insertion slots. Each culture chamber is equipped with a drain pipe at the bottom, and the drain pipe is equipped with a valve.

[0022] The method for studying the root division effect of plants using the experimental device of this utility model includes the following specific steps: 1) Select wheat seeds of uniform size for germination. Disinfect the seeds with 75% alcohol and soak them for 8 hours to promote germination. 2) Select wheat seeds of uniform size for hydroponic seedling cultivation. During the seedling cultivation process, keep the liquid level at half the height of the seeds, and then place them in a light incubator for cultivation. Day (12 h) / night (12 h), day (28℃) / night (25℃), humidity 65%, observe and replenish the culture medium every 24 h. 3) When the wheat grows to the point of having two leaves and one heart (about 9 to 10 days), the above-ground part is 12 to 15 cm tall, the root system is 15 to 20 cm long, and the number of fibrous roots is 6 to 8. This stage is the optimal treatment time for the wheat root division test. 4) Select healthy wheat seedlings with consistent above-ground and underground growth for treatment. Each seedling retains 4 roots (remove excess fibrous roots). Trim the 4 retained roots to the same length (about 12 cm). Then, separate the roots evenly and place them in the guide tubes of the planting cotton of this utility model. Fix the planting cotton in the middle of the partition. The roots are in an independent culture chamber through the guide tubes. Pour the nutrient solution of different treatments into each culture chamber, and the height should be level with the bottom of the planting cotton. 5) Place the culture room in a light incubator for cultivation, with daytime (12 h) / nighttime (12 h), daytime (28℃) / nighttime (25℃), and humidity of 65%. Observe and replenish the culture medium every 24 hours, and observe the root growth every day.

[0023] The embodiments described above are merely preferred solutions of this utility model and are not intended to limit this utility model in any way. Other variations and modifications are possible without departing from the technical solutions described in the claims.

[0024] In the description of this utility model, it should be understood that the terms indicating orientation or positional relationship are based on the orientation or positional relationship shown in the drawings and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

Claims

1. An experimental apparatus for studying the effect of root division in plants, characterized in that: The system includes a cultivation chamber with a partition that is detachably connected to the side wall of the cultivation chamber, dividing the cultivation chamber into two independent cultivation rooms. A planting cotton plant is installed on the top of the partition, and multiple sets of conduits are fixed on the planting cotton plant. Each set of conduits consists of two tubes, and the two tubes in each set are located in the two cultivation rooms respectively. Each cultivation room is equipped with an automatic nutrient solution circulation system and a water quality detector, which are electrically connected to a controller.

2. The experimental apparatus for studying the effect of root division in plants according to claim 1, characterized in that: The top of the partition is provided with a slot, and the planted cotton is attached to the slot.

3. The experimental apparatus for studying the effect of root division in plants according to claim 1, characterized in that: The automatic nutrient solution circulation system includes an inlet pipe and an outlet pipe installed on the side wall of the culture chamber. A water pump is connected between the inlet pipe and the outlet pipe. The water pump is electrically connected to a controller. Valves are provided on both the inlet pipe and the outlet pipe.

4. The experimental apparatus for studying the effect of root division in plants according to claim 1, characterized in that: The inner wall of the catheter is coated with a hydrophilic nano-coating.

5. The experimental apparatus for studying the effect of root division in plants according to claim 1, characterized in that: The culture chamber is equipped with a top cover.

6. The experimental apparatus for studying the effect of root division in plants according to claim 1, characterized in that: Each of the culture chambers is equipped with a root detector on its side wall, and the root detector is connected to a computer with a root analysis system installed.

7. The experimental apparatus for studying the effect of root division in plants according to claim 1, characterized in that: The side wall of the culture chamber is provided with a connector groove, and the partition is connected to the connector groove.

8. The experimental apparatus for studying the effect of root division in plants according to claim 1, characterized in that: The culture chamber and partition are both made of opaque material.

9. The experimental apparatus for studying the effect of root division in plants according to claim 1, characterized in that: Each of the culture chambers is equipped with a drain pipe at the bottom, and the drain pipe is equipped with a valve.

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