Urine stem cell protection solution storage and dispensing device

Abstract

The utility model relates to cell protection liquid storage and distribution device technical field, especially urine stem cell protection liquid storage and distribution device, including sealed cover and storage box, for the sealed cover of storing temperature insulation protection urine stem cell protection liquid storage and distribution, the sealed cover is equipped with the sealed cover for the protection of whole different storage container taking out and putting in corresponding buckle, through adopting semiconductor refrigeration stick combination jet pipe airflow circulation, realize zero minus temperature to zero plus temperature dynamic regulation, avoid the temperature fluctuation caused by traditional passive cooling, ensure stem cell long -term activity, magnetic attraction sealing plug and vibration sensor linkage, open the cover when automatic trigger pressure flash light alarm, prevent false operation and pollute sample, push rod air cylinder cooperation pressure sensor realizes sample tube accurate push, reduces the cell damage caused by manual intervention, detachable sample tube (with spring damper tension ring) support batch processing, adapt to different capacity demand, improve overall storage effect.

Description

Technical Field

[0001] This utility model relates to the technical field of cell preservation solution storage and preparation devices, and in particular to a urine stem cell preservation solution storage and preparation device. Background Technology

[0002] Urinary stem cells are adult stem cells isolated from urine. They have multi-directional differentiation potential and self-renewal capacity. Cell preservation solution is a special culture medium or preservation solution used to maintain the activity of urinary stem cells and promote their proliferation and differentiation. The stem cell preservation solution is stored and protected in a storage box.

[0003] Traditional urine stem cell preservation solution storage devices collect urine through a funnel and then allow it to flow into a storage bottle under gravity to mix with the preservation solution. They rely on passive sealing and natural temperature to maintain cell activity. However, due to temperature variations, they cannot adapt to the low-temperature environment required for long-term stem cell preservation. Repeated opening of the bottle is necessary for retesting, which increases the risk of cell damage and reduces the overall storage and preparation effect. Utility Model Content

[0004] To overcome the limitations of urine stem cell preservation solution storage devices that rely on passive sealing and natural temperature to maintain cell activity, which cannot adapt to the low-temperature environment required for long-term stem cell preservation due to temperature influences, and require repeated opening during re-examination, increasing the risk of cell damage, this utility model provides a urine stem cell preservation solution storage device.

[0005] The technical solution is as follows: A urine stem cell preservation solution storage and preparation device includes a sealing cap and a storage box. The sealing cap is used for storing and insulating the urine stem cell preservation solution. The sealing cap is also fitted with a corresponding sealing cap for protecting the removal and placement of different storage containers. The device includes a dispensing module, a cooling module, and sample tubes. The center of the sealing cap is provided with a dispensing module for dispensing the urine stem cell preservation solution into the storage and isolating it from contamination. The center of the storage box is provided with a cooling module for low-temperature storage and protection of the sample tubes. Several sets of sample tubes for storing stem cell preservation solution are distributed circumferentially around the dispensing module in the storage box.

[0006] Furthermore, a positioning ring is fixed to the sealing cap, and a retaining ring is provided at the bottom of the positioning ring. The retaining ring is provided in two sets. Several sets of retaining rings are fixed to the positioning ring in a circumferential direction. The center of the sealing cap is provided with a central insertion hole for positioning and installing the distribution module. Several sets of distribution tubes for adding urine stem cell protective solution are provided in a circumferential direction around the central insertion hole on the sealing cap. The distribution tubes have magnetic notches.

[0007] Furthermore, the storage box is equipped with several sets of thermometers extending into the interior in a circumferential manner on the outside. A shock-absorbing ring is fixed to the bottom of the storage box, and several sets of brackets are fixed to the shock-absorbing ring in a circumferential manner on the shock-absorbing ring. A corresponding retaining ring groove is opened at the top edge of the storage box, and several sets of push rods for pushing sample tubes are provided at the bottom of the storage box, with a cylinder at the bottom of the push rod.

[0008] Furthermore, the distribution module includes a sleeve and a swing arm. The center of the sleeve is provided with a steering rod, and the outer end of the steering rod is provided with several sets of swing arms in a circumferential direction. The top of the steering rod is connected to a drive motor, which drives the steering rod to rotate the swing arms.

[0009] Furthermore, a magnetic sealing plug is connected to the end of the steering rod away from the steering rod. A vibration sensor is located at the center of the magnetic sealing plug, and a pressure flashing light is electrically connected to the top of the vibration sensor.

[0010] Furthermore, the cooling module includes a control module, cooling rods, and injection pipes. The bottom of the control module is equipped with a connecting plate, and several sets of cooling rods are arranged circumferentially near the bottom edge of the connecting plate. The bottom of the cooling rods is equipped with an air exchange box, and several sets of air exchange slots are opened on the air exchange box. The cooling rods are equipped with a cooling pump that is electrically connected to the control module. The control module is equipped with a lithium battery pack and a wireless module.

[0011] Furthermore, the air exchange box is provided with several sets of airflow pipes in a circumferential manner. The end of the airflow pipe away from the air exchange box is provided with a conduit. The end of the conduit away from the airflow pipe is connected to a jet pipe. Several sets of jet grooves are opened on the jet pipe. A pressure pump is provided inside the jet groove. A temperature sensor is connected to the top of the jet pipe.

[0012] Furthermore, a guide cap is fastened to the sample tube, and a connecting cylinder is fixedly connected to the center of the guide cap. Several sets of pressure bars are arranged circumferentially near the edge of the guide cap. Pressure sensors are installed inside the pressure bars. A pressure plate connected to the push rod is fitted at the bottom of the sample tube. A tensioning ring for tensioning and protecting the sample tube is fitted on the pressure plate. The tensioning ring is set in two sets of symmetrical semi-rings. A spring column is provided between the two sets of semi-rings. A spring damper is installed inside the spring column.

[0013] The beneficial effects are: This utility model achieves dynamic adjustment of temperature from below zero to above zero by using a semiconductor cooling rod combined with jet pipe airflow circulation, avoiding temperature fluctuations caused by traditional passive cooling and ensuring the long-term activity of stem cells;

[0014] The magnetic sealing plug is linked to the vibration sensor, and automatically triggers a pressure flashing alarm when the lid is opened to prevent accidental contamination of the sample.

[0015] The push rod cylinder, in conjunction with a pressure sensor, enables precise delivery of sample tubes, reducing cell damage caused by manual intervention. The detachable sample tubes (with spring damper tension ring) support batch processing, adapting to different capacity requirements and improving overall storage efficiency. Attached Figure Description

[0016] Figure 1 This is a three-dimensional schematic diagram of a urine stem cell preservation solution storage and preparation device according to the present invention;

[0017] Figure 2This is an exploded view of the sealing cap and dispensing module of this utility model;

[0018] Figure 3 This is a schematic diagram of the storage box of this utility model;

[0019] Figure 4 This is a schematic diagram of the refrigeration module of this utility model;

[0020] Figure 5 This is a schematic diagram of the sample tube of this utility model.

[0021] In the attached diagram, the following are the reference numerals: 1. Sealing cap; 2. Storage box; 3. Dispensing module; 4. Cooling module; 5. Sample tube; 101. Positioning ring; 102. Snap-fit ​​ring; 103. Center insertion hole; 104. Dispensing tube; 105. Notch; 201. Thermometer; 202. Shock-absorbing ring; 203. Support; 204. Cylinder; 205. Ring groove; 206. Push rod; 301. Drive motor; 302. Swing arm; 303. Magnetic... 304. Suction plug; 305. Pressure flashing light; 401. Steering rod; 402. Control module; 403. Cooling rod; 404. Airflow pipe; 405. Conduit; 406. Temperature sensor; 407. Injection pipe; 408. Injection groove; 409. Air exchange box; 501. Air exchange groove; 502. Guide cover; 503. Connecting cylinder; 504. Pressure rod; 505. Tensioning ring; 506. Spring column; 507. Pressure plate. Detailed Implementation

[0022] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0023] Basic ingredients

[0024] It contains nutrients such as vitamins, minerals, amino acids, and growth factors, providing metabolic support for stem cells.

[0025] It may contain nano-sized colloidal concentrates to enhance nutrient absorption efficiency.

[0026] Functional design

[0027] Reduce cell damage and prolong stem cell survival time by using antioxidants (such as active substances that scavenge free radicals).

[0028] Simulates the in vivo microenvironment to maintain the multi-directional differentiation potential and self-renewal capacity of stem cells.35

[0029] Main function

[0030] Cell viability protection

[0031] During the transportation or storage of urine samples, prevent stem cells from becoming inactive due to environmental changes to ensure the success rate of subsequent culture.

[0032] Promote proliferation and differentiation

[0033] Provide growth factors (such as EGF, FGF, etc.) to accelerate stem cell proliferation and support their differentiation into target cell types (such as kidney cells, nerve cells, etc.).

[0034] Clinical application assistance

[0035] Before stem cell therapy, pretreatment with a protective solution can improve cell quality and enhance tissue repair effects (such as kidney repair and skin regeneration).

[0036] Sealing optimization

[0037] The ring-shaped silicone tube is designed to fit the urethral opening, reducing contamination; the storage tube features a double-sealed structure to prevent the protective solution from evaporating.

[0038] Activity maintenance design

[0039] The stirring shaft works in synergy with the cooling monocrystalline silicon to pre-cool the protective liquid to 4°C before injecting stem cells, thus avoiding damage from sudden temperature changes.

[0040] Modular extension

[0041] The stackable tray structure supports batch sample processing, and the isolation mechanism reduces heat exchange when the lid is opened.

[0042] Potential Applications

[0043] Current research largely focuses on direct treatment with urinary stem cells, with protective solutions serving more as complementary technologies. However, their optimization is crucial for improving the reliability and efficiency of cell therapy.

[0044] Biological characteristics of urine stem cells

[0045] Urinary stem cells are adult stem cells isolated from urine, possessing multi-lineage differentiation potential (such as differentiating into neurons, kidney cells, etc.) and self-renewal capacity. Their advantages include:

[0046] Non-invasive collection: Urine is a human excretory substance, and the collection process is painless and repeatable;

[0047] High activity: Studies have shown that urine stem cells proliferate rapidly in vitro and have a higher differentiation efficiency than some traditionally derived stem cells.

[0048] Functional requirements of protective fluid

[0049] Cell preservation solutions must meet the following core requirements:

[0050] Activity maintenance: Contains antioxidants (such as glutathione) and nutritional factors (such as FGF-2), which delay cell apoptosis;

[0051] Low temperature compatibility: Maintains chemical stability in the range of 4℃ to -196℃, avoiding freezing damage;

[0052] Contamination-resistant design: The combination of aseptic formula and sealed storage prevents microbial contamination.

[0053] Traditional structure and working principle

[0054] Basic structure:

[0055] It consists of a urine collection bottle (made of transparent glass or plastic), sealing components (such as rubber rings and threaded interfaces), and a simple temperature control module. Some devices are equipped with scale markings for easy quantitative collection.

[0056] It adopts a layered design, with the upper layer being a urine collection funnel (made of corrosion-resistant polypropylene) and the lower layer being a pre-storage chamber for protective liquid. Liquid mixing is controlled by a push-pull switch.

[0057] Working principle:

[0058] After being collected through a funnel, the urine flows into a urine storage bottle by gravity and mixes with the protective solution. Cell viability is maintained by passive sealing and natural temperature (usually only supports short-term storage at 4°C).

[0059] Structural defects

[0060] Insufficient sealing: Traditional rubber rings are prone to aging, leading to the evaporation or contamination of the protective fluid; push-pull switches pose a risk of backflow, affecting sample purity.

[0061] Weak temperature control: Lacking an active cooling / heating module, it cannot adapt to the low-temperature environment (such as -80℃ or liquid nitrogen conditions) required for long-term stem cell preservation.

[0062] Operational complexity: It requires manual mixing of the protective solution and urine, which can easily introduce human error; repeated opening of the cap is required during retesting, which increases the risk of cell damage.

[0063] performance bottleneck

[0064] Low cell survival rate: Repeated opening and closing of the lid leads to temperature fluctuations, which reduces stem cell activity by more than 30%.

[0065] Poor scalability: Fixed capacity design is difficult to adapt to the needs of batch sample processing.

[0066] Integration and intelligence

[0067] The future device will integrate the Internet of Things to enable remote monitoring and integrate a liquid nitrogen tank interface to adapt to cryogenic transport.

[0068] Standardization Promotion

[0069] With the improvement of the "Technical Guidelines for Cell Therapy Products", urine stem cell storage devices need to pass GMP certification to ensure compliance throughout the entire process from collection to storage.

[0070] In summary, the core of the technological innovation of the urine stem cell preservation solution storage device lies in solving the synergistic problems of sealing, temperature control accuracy, and operational efficiency, providing a more reliable cell preservation solution for regenerative medicine.

[0071] like Figures 1-5 As shown, a urine stem cell preservation solution storage and preparation device includes a sealing cap 1 and a storage box 2. The sealing cap 1 is used for storing and insulating the urine stem cell preservation solution. The sealing cap 1 is also fitted with a corresponding sealing cap for protecting the removal and placement of different storage containers. The device also includes a dispensing module 3, a cooling module 4, and sample tubes 5. The sealing cap 1 has a dispensing module 3 at its center for dispensing the urine stem cell preservation solution into the storage and isolating it from contamination. The storage box 2 has a cooling module 4 at its center for low-temperature storage protection of the sample tubes 5. Several sets of sample tubes 5 for storing stem cell preservation solution are distributed circumferentially around the dispensing module 3 in the storage box 2.

[0072] Please see Figures 2-4 In this embodiment, a positioning ring 101 is fixedly connected to the sealing cover 1. The bottom of the positioning ring 101 is provided with a retaining ring, which is set in two sets. Several sets of retaining rings 102 are fixedly connected to the positioning ring 101 in a circumferential direction. The center of the sealing cover 1 is provided with a central insertion hole 103 for positioning and installing the distribution module 3. Several sets of dispensing tubes 104 for adding urine stem cell protective solution are provided in a circumferential direction on the sealing cover 1 with the central insertion hole 103 as the center. The dispensing tubes 104 have magnetic suction notches 105. Several sets of thermometers 201 that penetrate into the interior are provided in a circumferential direction on the outside of the storage box 2. A shock-absorbing ring 202 is fixedly connected to the bottom of the storage box 2. Several sets of brackets 203 are fixedly connected to the shock-absorbing ring 202 in a circumferential direction. A ring groove 205 corresponding to the retaining ring is opened at the top edge of the storage box 2. Several sets of push rods 206 for pushing sample tubes 5 are provided at the bottom of the storage box 2. A cylinder 204 is provided at the bottom of the push rod 206.

[0073] Please see Figures 3-4 In this embodiment, the distribution module 3 includes a sleeve and a swing arm 302. The center of the sleeve is provided with a steering rod 305. Several sets of swing arms 302 are provided circumferentially at the outer end of the steering rod 305. The top of the steering rod 305 is connected to a drive motor 301. The drive motor 301 drives the steering rod 305 to drive the swing arms 302 to turn. The end of the steering rod 305 away from the steering rod 305 is connected to a magnetic sealing plug 303 (with built-in N52 neodymium magnet). The center of the magnetic sealing plug 303 is provided with a vibration sensor, model (SW-420). The top of the vibration sensor is electrically connected to a pressure flashing lamp 304.

[0074] Please see Figures 4-5In this embodiment, the cooling module 4 includes a control module 401, cooling rods 402, and injection pipes 406. The bottom of the control module 401 has a connecting plate, and several sets of cooling rods 402 (model TEC1-12706) are arranged circumferentially near the edge of the bottom of the connecting plate. A ventilation box 408 is located at the bottom of the cooling rods 402, and several ventilation slots 409 are formed on the ventilation box 408. A cooling pump (model MCP655) electrically connected to the control module 401 is located inside the cooling rods 402. The control module 401 is a main control chip STM32F103, which contains a lithium battery pack and a wireless module (model ESP8266). Several airflow pipes 403 are arranged circumferentially on the ventilation box 408, and a conduit 404 is provided at the end of each airflow pipe 403 away from the ventilation box 408. 3. One end is connected to a spray pipe 406, model PM2401B. The spray pipe 406 has several sets of spray grooves 407. The spray grooves 407 are equipped with a pressure pump. The top of the spray pipe 406 is connected to a temperature sensor 405, model DS18B20. The sample tube 5 is fastened with a guide cover 501. The center of the guide cover 501 is fixedly connected to a connecting cylinder 502. Several sets of pressure rods 503 are arranged circumferentially near the edge of the guide cover 501. The pressure rods 503 are equipped with a pressure sensor, model FSR402. The bottom of the sample tube 5 is fitted with a pressure plate 506 connected to the push rod 206. The pressure plate 506 is fitted with a tension ring 504 to protect the sample tube 5 from tension. The tension ring 504 is set with two sets of symmetrical semi-rings. A spring column 505 is set between the two sets of semi-rings. A spring damper is set inside the spring column 505.

[0075] Open the overall sealing cover 1, place sample tubes of different sizes into the tension ring 504, close the sealing cover 1, push rod 206 and cylinder 204 lift the pressure plate 506, tension ring 504 automatically clamps the sample tube 5 to prevent displacement during transportation, pressure rod 503 detects the tube cover closure status, cooling rod 402 transfers cold energy to air exchange box 408 through cooling pump, airflow pipe 403 (diameter 8mm) guides cold air through spray groove 407 (flow rate 2m / s) to evenly cover sample tube 5, temperature sensor 405 feeds back data to control module 401 every 10 seconds, drive motor 301 drives steering rod 305 to rotate and align with notch 105 of distribution tube 104, the magnetic adsorption force reaches 5kg / cm during sealing. 2 To ensure airtightness, after the vibration sensor detects abnormal vibration, it sends an alarm to the control terminal via the wireless module, causing the swing arm 302 to inject the protective liquid from the distribution tube 104 into the sample tube 5. The flow rate is monitored in real time by the pressure sensor.

[0076] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A urine stem cell preservation solution storage and preparation device, comprising a sealing cap (1) and a storage box (2), wherein the sealing cap (1) is used for storing and insulating the urine stem cell preservation solution, and the sealing cap (1) is correspondingly fitted with a sealing cap (1) for protecting the removal and placement of different storage containers; characterized in that: It also includes a dispensing module (3), a cooling module (4) and sample tubes (5). The center of the sealing cap (1) is provided with a dispensing module (3) for dispensing urine stem cell protective solution into storage and isolating it from contamination. The center of the storage box (2) is provided with a cooling module (4) for low-temperature storage protection of the sample tubes (5). Several groups of sample tubes (5) for storing stem cell protective solution are distributed in a circumferential manner around the dispensing module (3) in the storage box (2).

2. The urine stem cell preservation solution storage and preparation device according to claim 1, characterized in that, A positioning ring (101) is fixedly connected to the sealing cap (1). The bottom of the positioning ring (101) is provided with a retaining ring. The retaining ring is provided in two sets. Several sets of retaining rings (102) are fixedly connected to the positioning ring (101) in a circumferential direction. The center of the sealing cap (1) is provided with a central insertion hole (103) for the positioning installation and distribution module (3). Several sets of distribution tubes (104) for adding urine stem cell protection solution are provided in a circumferential direction on the sealing cap (1) with the central insertion hole (103) as the center. The distribution tubes (104) have magnetic suction notches (105).

3. The urine stem cell preservation solution storage and preparation device according to claim 2, characterized in that, The storage box (2) is provided with several sets of thermometers (201) extending into the outside. The bottom of the storage box (2) is fixed with a shock-absorbing ring (202). Several sets of brackets (203) are fixed on the shock-absorbing ring (202). The top edge of the storage box (2) is provided with a corresponding retaining ring groove (205). The bottom of the storage box (2) is provided with several sets of push rods (206) for pushing sample tubes (5). The bottom of the push rods (206) is provided with a cylinder (204).

4. The urine stem cell preservation solution storage and preparation device according to claim 1, characterized in that, The distribution module (3) includes a socket and a swing arm (302). The center of the socket is provided with a steering rod (305). Several sets of swing arms (302) are provided around the outer end of the steering rod (305). A drive motor (301) is connected to the top of the steering rod (305). The drive motor (301) drives the steering rod (305) to drive the swing arm (302) to turn.

5. The urine stem cell preservation solution storage and preparation device according to claim 4, characterized in that, A magnetic sealing plug (303) is connected to the end of the steering rod (305) away from the steering rod (305). A vibration sensor is provided at the center of the magnetic sealing plug (303), and a pressure flashing lamp (304) is electrically connected to the top of the vibration sensor.

6. The urine stem cell preservation solution storage and preparation device according to claim 1, characterized in that, The refrigeration module (4) includes a control module (401), a refrigeration rod (402), and a jet pipe (406). The bottom of the control module (401) is provided with a connecting plate. Several sets of refrigeration rods (402) are arranged circumferentially near the edge of the bottom of the connecting plate. The bottom of the refrigeration rod (402) is provided with a ventilation box (408). Several sets of ventilation slots (409) are opened on the ventilation box (408). The refrigeration rod (402) is provided with a refrigeration pump electrically connected to the control module (401). The control module (401) is provided with a lithium battery pack and a wireless module.

7. The urine stem cell preservation solution storage and preparation device according to claim 6, characterized in that, The air exchange box (408) is provided with several sets of airflow pipes (403) in a circumferential manner. The end of the airflow pipe (403) away from the air exchange box (408) is provided with a conduit (404). The end of the conduit (404) away from the airflow pipe (403) is connected to a jet pipe (406). Several sets of jet grooves (407) are opened on the jet pipe (406). A pressure pump is provided inside the jet groove (407). A temperature sensor (405) is connected to the top of the jet pipe (406).

8. The urine stem cell preservation solution storage and preparation device according to claim 3, characterized in that, A guide cover (501) is fastened to the sample tube (5). A connecting cylinder (502) is fixedly connected to the center of the guide cover (501). Several sets of pressure rods (503) are arranged circumferentially near the edge of the guide cover (501). A pressure sensor is installed inside the pressure rod (503). A pressure plate (506) connected to the push rod (206) is fitted at the bottom of the sample tube (5). A tensioning ring (504) for tensioning and protecting the sample tube (5) is fitted on the pressure plate (506). The tensioning ring (504) is set in two sets of symmetrical semi-rings. A spring column (505) is provided between the two sets of semi-rings. A spring damper is installed inside the spring column (505).

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