A column loader for chromatography columns

Abstract

This application provides a column packer for chromatography, belonging to the field of chromatography column technology. The column packer includes a packer section and an auxiliary flow guide assembly. The chromatography tube is rotatably clamped onto a support; a support column is connected to the support, and a sliding collar is slidably fitted onto the support column. An adjusting element is provided on the sliding collar, and the adjusting element is tightly abutted against the side wall of the support column. A hanger is fixedly connected to the sliding collar, and the chromatography tube is rotatably positioned directly below the hanger. The adjustment element, in conjunction with the sliding collar, allows the solution to be placed at a low position and then raised, eliminating the need for the experimenter to constantly hold the solution for pouring, improving the continuity of solution pouring while reducing the operational difficulty for the experimenter. Furthermore, the solution remains placed on the hanger, eliminating the need for frequent handling.

Description

Technical Field

[0001] This application relates to the field of chromatographic columns, and more specifically, to a column packer for a chromatographic column. Background Technology

[0002] Glycated hemoglobin (HbA1c) is widely used in clinical practice as an indicator of diabetes. It is a product of an irreversible enzymatic reaction between human hemoglobin and glucose in the blood, reflecting the average blood glucose level over 2-3 months, and the results are unaffected by food intake. Glycated hemoglobin chromatography, using high-performance liquid chromatography (HPLC), is the gold standard for determining HbA1c levels in human blood and has a wide range of clinical applications.

[0003] The separation of HbA1c and non-glycated hemoglobin is based on their different charges. Non-glycated hemoglobin carries a positive charge, while glycated hemoglobin is almost uncharged; they are separated according to their different charge properties. The stationary phase used in this method is a cation-exchange stationary phase, which contains groups that can exchange cations. This stationary phase can bind to the positively charged non-glycated hemoglobin through electrostatic interactions, while glycated hemoglobin, being uncharged, cannot bind to the stationary phase. Eluent is first used at a low pH, where glycated hemoglobin is eluted first; then, a high pH eluent is used to elute non-glycated hemoglobin, yielding the corresponding hemoglobin chromatographic profile.

[0004] During the column packing process, glycated hemoglobin reagent needs to be manually poured into the column, and its design directly affects the separation effect, column efficiency, and reproducibility. Classic packing methods include dry packing and wet packing. Dry packing is simple to operate and results in less solvent loss, but it is prone to generating bubbles, affecting packing and separation performance. Wet packing requires an additional container for mixing the packing material, which is relatively difficult to implement on a large scale and also easily leads to the loss of both packing solvent and packing material.

[0005] For example, a column packer for rapid column packing disclosed in Chinese application number CN109358146A provides pressure through hydraulic pressure when the pressure rod is pressurized during the packing process, which helps to increase the pressure and ensure pressure stability, thus improving the packing quality. After pressurization is completed, the pressure rod is moved upward by air pressure, which effectively speeds up the retraction of the pressure rod and improves the packing efficiency. However, in the laboratory, the pre-packing process is usually done manually, which requires guiding the flow of the solution through a glass rod. That is, one hand holds the position of the glass rod while the other hand pours the solution onto the glass rod. However, manual column packing is prone to uneven force or discontinuous solution pouring, and it is not easy to operate due to the height of the chromatography tube. Utility Model Content

[0006] To overcome the above shortcomings, this application provides a column packer for chromatography columns, which aims to improve the problem of high chromatography tube height and difficulty in operation.

[0007] This application provides a column packer for a chromatography column, including a column packer part and an auxiliary drainage component.

[0008] The column packing unit includes a support and a chromatography tube, wherein the chromatography tube is rotatably clamped on the support;

[0009] An auxiliary drainage component includes a support column connected to the bracket. A sliding collar is slidably fitted on the support column, and an adjustment element is provided on the sliding collar. The adjustment element is tightly pressed against the side wall of the support column. A hanger is fixedly connected to the sliding collar, and the chromatography tube is rotatably located directly below the hanger.

[0010] In one specific implementation, a pressure bar is detachably mounted on the bracket, and the pressure bar is located inside the chromatography tube to apply pressure.

[0011] In one specific implementation, a liquid-containing bottle is detachably hung on the bracket, and a conduit is provided on the liquid-containing bottle, the conduit extending into the interior of the chromatography tube.

[0012] In one specific implementation, the top of the pressure bar is provided with a first hook, which is detachably hung on a hanger.

[0013] In one specific implementation, a second hook is provided on the top of the liquid bottle, and the second hook can be detachably hung on a hanger.

[0014] In one specific implementation, the adjusting element includes a knob, which is fixedly connected to a threaded rod, which is threaded through the sliding collar and abuts against the side wall of the support column.

[0015] In one specific implementation, a connecting rod is provided between the support column and the bracket.

[0016] In one specific implementation, a clamping member is rotatably connected to the support, and the chromatography tube is clamped by the clamping member.

[0017] Beneficial effects: The adjustable mechanism combined with the sliding collar allows the solution to be placed at a low position and then raised, eliminating the need for the operator to constantly hold the solution while pouring. This improves the continuity of solution pouring and reduces the difficulty of operation for the operator. Furthermore, the solution remains on the rack without requiring frequent handling. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0019] Figure 1 This is a first-view structural schematic diagram of the first embodiment of the column packer for chromatography provided in this application;

[0020] Figure 2 A schematic diagram of the second view of the first embodiment of the column packer for the chromatography column provided in this application;

[0021] Figure 3 A first-view structural schematic diagram of a second embodiment of a column packer for chromatography provided in this application;

[0022] Figure 4 A second-view structural schematic diagram of a column packer for a chromatography column provided in this application embodiment;

[0023] Figure 5 A schematic diagram illustrating the connection structure between the sliding collar and the hanger provided in the embodiments of this application;

[0024] Figure 6 This is a schematic diagram of the connection structure between the adjusting member and the sliding collar provided in the embodiments of this application.

[0025] In the diagram: 10 - Column packer; 110 - Support; 120 - Clamping element; 130 - Chromatography tube; 140 - Base; 20 - Auxiliary drainage assembly; 210 - Support column; 220 - Sliding collar; 230 - Pressure rod; 240 - Adjusting element; 241 - Knob; 242 - Threaded rod; 250 - Connecting rod; 260 - Liquid collection bottle; 270 - Tubing tube; 280 - Hanger. Detailed Implementation

[0026] The technical solutions in the embodiments of this application will now be described with reference to the accompanying drawings.

[0027] Example 1

[0028] Please see Figures 1-6 This application provides a column packer for a chromatography column, including a column packer portion 10 and an auxiliary drainage assembly 20.

[0029] Please see Figure 1 , Figure 2 , Figure 3 and Figure 4The column packer section 10 includes a support 110 and a chromatography tube 130, with the chromatography tube 130 rotatably clamped onto the support 110. Specifically, a base 140 is provided at the bottom of the support 110. A clamping member 120 is rotatably connected to the support 110, and the chromatography tube 130 is clamped by the clamping member 120. During column packing, the chromatography tube 130 is first clamped onto the clamping member 120, and then the column packing sequence is followed.

[0030] Please see Figure 1 , Figure 2 , Figure 5 and Figure 6 The auxiliary drainage component 20 includes a support column 210 connected to a bracket 110. A sliding collar 220 is slidably fitted onto the support column 210, and an adjusting member 240 is provided on the sliding collar 220, which abuts against the side wall of the support column 210. A hanger 280 is fixedly connected to the sliding collar 220, and the chromatography tube 130 is rotatably positioned directly below the hanger 280. A liquid-holding bottle 260 is detachably hung on the hanger 280, and a conduit 270 is provided on the liquid-holding bottle 260, extending into the interior of the chromatography tube 130. A second hook is provided at the top of the liquid-holding bottle 260, and the second hook is detachably hung on the hanger 280. When liquid needs to be poured during column loading, first loosen the adjusting component 240, slide the sliding collar 220 downwards, and then hang the liquid bottle 260 on the hanger 280 via the second hook. Next, move the sliding collar 220 upwards. At this point, rotate the chromatography tube 130 so that it is positioned directly above the liquid bottle 260. Then, insert the tubing 270 into the liquid bottle 260 and place the other end of the tubing 270 into the chromatography tube 130, ensuring the tubing 270 is flush against the inner wall of the tube for solution flow. The adjustment component 240, in conjunction with the sliding collar 220, allows the solution to be placed at a low position and then raised, eliminating the need for the operator to constantly hold the solution while pouring. This improves the continuity of solution pouring while reducing the operator's workload. Furthermore, the solution remains on the hanger 280, eliminating the need for frequent handling.

[0031] In this embodiment, the adjusting component 240 includes a knob 241, which is fixedly connected to a threaded rod 242. The threaded rod 242 is screwed through the sliding collar 220 and abuts against the side wall of the support column 210. When the sliding collar 220 needs to be moved up or down, the knob 241 is loosened, causing the threaded rod 242 to disengage from the sliding collar 220, allowing for movement. When fixing is required, the knob 241 is turned, causing the threaded rod 242 to abut against the outer wall of the sliding collar 220, thus fixing the hanger 280.

[0032] In a specific configuration, a connecting rod 250 is provided between the support column 210 and the bracket 110.

[0033] Example 2

[0034] The difference from Example 1 is that:

[0035] In this embodiment, a pressure rod 230 is detachably mounted on the bracket 280, and the pressure rod 230 is located inside the chromatography tube 130 for pressurization. A first hook is provided at the top of the pressure rod 230, and the first hook is detachably mounted on the bracket 280. The bracket 280 can also be used to pressurize the inside of the chromatography tube 130. When pressurization is needed, the pressure rod 230 is first placed inside the chromatography tube 130, then the chromatography tube 130 is rotated so that the pressure rod 230 is directly below the bracket 280. Then, the first hook is hooked onto the bracket 280, and the sliding collar 220 is moved downwards, thereby moving the pressure rod 230 downwards and applying pressure to the inside of the chromatography tube 130. The detachable mounting of the pressure rod 230 on the bracket 280 allows for pressure application to the chromatography tube 130 with less effort, while also reducing equipment operating costs.

[0036] The working principle of the packer for this chromatography column:

[0037] When liquid needs to be poured during column loading, first loosen the adjusting member 240, slide the sliding collar 220 downwards, and then hang the liquid bottle 260 on the hanger 280 via the second hook. Next, move the sliding collar 220 upwards. At this point, rotate the chromatography tube 130 so that it is positioned directly above the liquid bottle 260. Then, insert the tubing 270 into the liquid bottle 260 and place the other end of the tubing 270 into the chromatography tube 130, ensuring the tubing 270 is flush against the inner wall of the tube for solution flow. The adjustment member 240, in conjunction with the sliding collar 220, allows the solution to be placed at a low position and then raised, eliminating the need for the operator to constantly hold the solution for pouring. This improves the continuity of solution pouring while reducing the operator's workload. Furthermore, the solution remains on the hanger 280, eliminating the need for frequent handling.

[0038] The above description is merely an embodiment of this application and is not intended to limit the scope of protection of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of protection of this application. It should be noted that similar reference numerals and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

Claims

1. A packer for a chromatography column, characterized in that, include The column packer section (10) includes a support (110) and a chromatography tube (130), wherein the chromatography tube (130) is rotatably clamped on the support (110); The auxiliary drainage component (20) includes a support column (210) connected to the bracket (110). A sliding collar (220) is slidably sleeved on the support column (210). An adjusting member (240) is provided on the sliding collar (220) and the adjusting member (240) is tightly abutted against the side wall of the support column (210). A hanger (280) is fixedly connected to the sliding collar (220), and the chromatography tube (130) is rotatably located directly below the hanger (280).

2. The packer for a chromatography column according to claim 1, characterized in that, A pressure rod (230) is detachably hung on the bracket (280), and the pressure rod (230) is located inside the chromatography tube (130) for pressurization.

3. The packer for a chromatography column according to claim 1, characterized in that, A liquid-holding bottle (260) is detachably hung on the hanger (280), and a conduit (270) is provided on the liquid-holding bottle (260), which extends into the interior of the chromatography tube (130).

4. A packer for a chromatography column according to claim 2, characterized in that, The top of the pressure bar (230) is provided with a first hook, which can be detachably hung on the hanger (280).

5. A packer for a chromatography column according to claim 3, characterized in that, The liquid bottle (260) is provided with a second hook at the top, which can be detachably hung on the hanger (280).

6. A packer for a chromatography column according to claim 1, characterized in that, The adjusting component (240) includes a knob (241), which is fixedly connected to a threaded rod (242). The threaded rod (242) is screwed through the sliding collar (220) and abuts against the side wall of the support column (210).

7. A packer for a chromatography column according to claim 1, characterized in that, A connecting rod (250) is provided between the support column (210) and the bracket (110).

8. A packer for a chromatography column according to claim 1, characterized in that, A clamping member (120) is rotatably connected to the support (110), and the chromatography tube (130) is clamped by the clamping member (120).

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