A cell bilayer filtration device

Abstract

The utility model relates to a cell double -deck filtering device belongs to cell filtering equipment technical field, the device includes the first filter box and second filter box through the liquid transfer pipe intercommunication, and the first filter box side wall is equipped with fixed shaft, and the front wall lower extreme is equipped with the opening, and the fixed shaft on the opening is swinged to be connected with the pivot, and the pivot one end is fixed with first filter assembly, the first filter box upper end is equipped with the liquid inlet, and the liquid inlet pipe on the intercommunication inner wall, and the liquid inlet pipe bottom is equipped with the gasket. The first filter assembly includes ring pipe, sleeve, bolt and the first filter screen of 70um aperture, and the second filter box is equipped with second filter assembly, including ring pipe, sleeve, bolt and the second filter screen of 40um aperture. The fixed shaft is equipped with the buckle structure at pivot junction, and the second filter box bottom is equipped with the liquid collecting pipe connection liquid transfer bag. The utility model can effectively separate the cell of different size, improve cell separation purity and efficiency, and convenient maintenance and filter screen replacement.

Description

Technical Field

[0001] This utility model relates to the field of cell filtration devices, specifically to a cell double-layer filtration device. Background Technology

[0002] Cell filtration is a crucial step in biomedical research and clinical applications, used to separate, purify, and collect specific cell types. With the advancement of biomedical technology, the requirements for cell filtration devices are becoming increasingly stringent, particularly in terms of filtration efficiency, precision, and ease of use.

[0003] Existing cell filtration devices suffer from the following problems: First, they mostly employ a single-layer filtration structure, which cannot meet the needs of graded filtration of cells with different particle sizes. Second, during use, the filter screen is prone to filtration blockage or clogging, affecting filtration efficiency. Once clogged, the filter screen needs cleaning, but the existing device's filter screen design is inconvenient for maintenance and cleaning, increasing operating costs and difficulty. Therefore, there is an urgent need for a cell filtration device that can achieve multi-layer filtration and is easy to maintain and clean, in order to improve the efficiency and precision of cell filtration. Utility Model Content

[0004] The technical problem to be solved by this utility model is that, in the existing technology, most cell filters use single-layer filtration, which cannot meet the filtration requirements; and the filter screen becomes clogged or obstructed after use, requiring cleaning and making maintenance inconvenient. Therefore, this utility model provides a cell double-layer filtration device.

[0005] The technical solution adopted by this utility model to solve its technical problem is as follows: a cell double-layer filtration device is provided, including a first filter box and a second filter box. The first filter box and the second filter box are connected by an infusion tube. A fixed shaft is provided at the side wall connection of the first filter box. An opening is provided through the lower end of the front wall of the first filter box. A rotating shaft is movably connected to the fixed shaft at the opening. A first filter component is fixedly provided at one end of the rotating shaft. An inlet is provided through the upper end of the first filter box. An inlet tube fixed to the inner wall of the upper end of the first filter box is connected to the lower end of the inlet. A sealing gasket is provided at the bottom end of the inlet tube. The first filter component is movably provided at the lower end of the sealing gasket.

[0006] Furthermore, the first filter assembly includes a ring tube, a sleeve, bolts, and a first filter screen. The sleeve is disposed inside the ring tube, and the bottom of the sleeve is fixed to the bottom end of the ring tube by bolts. The first filter screen is placed inside the sleeve, and the pore size of the first filter screen is 70μm.

[0007] Furthermore, the second filter box is also provided with a second filter assembly, which includes a ring tube, a sleeve, and bolts. The second filter assembly also includes a second filter screen with a pore size of 40μm.

[0008] Furthermore, two sets of latches are provided at the movable connection between the fixed shaft and the rotating shaft, a slot is provided on the rotating shaft, a movable groove is provided at the connection between the fixed shaft and the latches, and a buffer spring is provided at the bottom of the latches, with the bottom of the buffer spring connected to the inner side of the slot.

[0009] Furthermore, a liquid collection tube is provided at the bottom of the second filter box, and an infusion bag is provided at the lower end of the liquid collection tube.

[0010] The beneficial effects of this invention are as follows: by employing two types of filter screens with pore sizes of 70μm and 40μm, cells are filtered in stages, meeting filtration requirements; the rotation of the shaft drives the first and second filter components to rotate, allowing the filter components to be rotated out for easy maintenance; the bottom of the sleeve is fixed to the bottom of the ring tube with bolts, and the sleeve can be pulled out after loosening the bolts for easy filter replacement; the buckle on the fixed shaft can fix the rotating shaft to prevent loosening, improving the stability and service life of the device. Compared with the prior art, this invention has a simple structure, is easy to operate, has low maintenance costs, and provides good filtration effect. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0012] Figure 2 This is a schematic diagram of the internal structure of the first filter box of this utility model;

[0013] Figure 3 This is a schematic diagram of the structure of the first filter component of this utility model after it has been pulled out.

[0014] Figure 4 This is an exploded structural diagram of the first filter component of this utility model;

[0015] Figure 5 This is a schematic diagram of the internal structure of the second filter box of this utility model;

[0016] Figure 6 This is a schematic diagram of the internal structure of the buckle and fixed shaft connection of this utility model.

[0017] Legend:

[0018] 1-First filter box, 101-Inlet, 102-Fixed shaft, 103-Rotating shaft, 104-Opening, 105-First filter assembly, 1051-Ring tube, 1052-Sleeve, 1053-Bolt, 1054-First filter screen, 106-Sealing gasket, 107-Inlet pipe, 2-Second filter box, 201-Second filter assembly, 2011-Second filter screen, 3-Infusion bag, 4-Collection tube, 5-Infusion tube, 6-Slot, 7-Snap fastener, 8-Buffer spring, 9-Moveable groove. Detailed Implementation

[0019] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings and embodiments. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0020] Example

[0021] A cell double-layer filtration device includes a first filter box 1 and a second filter box 2, which are connected by an infusion tube 5. A fixed shaft 102 is provided at the side wall connection of the first filter box 1. An opening 104 is provided through the lower end of the front wall of the first filter box 1. A rotating shaft 103 is movably connected to the fixed shaft 102 at the opening 104. A first filter assembly 105 is fixedly provided at one end of the rotating shaft 103. An inlet 101 is provided through the upper end of the first filter box 1. An inlet pipe 107 is connected to the lower end of the inlet 101 and fixed to the inner wall of the upper end of the first filter box 1. A sealing gasket 106 is provided at the bottom end of the inlet pipe 107. The first filter assembly 105 is movably provided at the lower end of the sealing gasket 106.

[0022] The first filter assembly 105 includes a ring tube 1051, a sleeve 1052, a bolt 1053, and a first filter screen 1054. The sleeve 1052 is disposed inside the ring tube 1051. The bottom of the sleeve 1052 is fixed to the bottom end of the ring tube 1051 by the bolt 1053. The first filter screen 1054 is placed inside the sleeve 1052. The pore size of the first filter screen 1054 is 70μm.

[0023] The second filter box 2 is also provided with a second filter assembly 201, which includes a ring tube 1051, a sleeve 1052, and a bolt 1053. The second filter assembly 201 also includes a second filter screen 202 with a pore size of 40μm.

[0024] Two sets of latches 7 are provided at the movable connection between the fixed shaft 102 and the rotating shaft 103. The rotating shaft 103 is provided with a slot 6. The connection between the fixed shaft 102 and the latches 7 is provided with a movable groove 9. A buffer spring 8 is provided at the bottom of the latches 7. The bottom of the buffer spring 8 is connected to the inside of the slot 6.

[0025] The bottom of the second filter box 2 is provided with a liquid collection tube 4, and an infusion bag 3 is provided at the lower end of the liquid collection tube 4.

[0026] The cell dual-layer filtration device in this embodiment employs a dual-layer filtration structure, which can effectively separate cells of different sizes. The first filter component 105 in the first filter box 1 uses a first filter screen 1054 with a pore size of 70 μm, which can filter out larger impurities and cell clumps while retaining the desired cells. The second filter component 201 in the second filter box 2 uses a second filter screen 202 with a pore size of 40 μm, which can further filter out medium-sized cells and impurities, achieving a finer separation.

[0027] The cell dual-layer filtration device operates as follows: First, a liquid sample containing cells is poured into the first filter box 1 through the inlet 101 at the top. The liquid enters the first filter box 1 through the inlet pipe 107. When the liquid comes into contact with the first filter screen 1054 in the first filter assembly 105, cells and impurities larger than 70 μm are intercepted above the first filter screen 1054, while cells and liquid smaller than 70 μm pass through the first filter screen 1054 and flow into the bottom of the first filter box 1.

[0028] The liquid, after the first filtration, flows into the second filter box 2 through the infusion tube 5 connecting the first filter box 1 and the second filter box 2. In the second filter box 2, the liquid is filtered again through the second filter screen 202 in the second filter assembly 201. Since the pore size of the second filter screen 202 is 40μm, cells larger than 40μm but smaller than 70μm are intercepted above the second filter screen 202, while cells smaller than 40μm and the liquid pass through the second filter screen 202 and flow out from the collection tube 4 at the bottom of the second filter box 2, and are finally collected in the infusion bag 3.

[0029] The cell double-layer filtration device in this embodiment has a reasonable structural design and is easy to use. The lower end of the front wall of the first filter box 1 has an opening 104, and the first filter assembly 105 is movably connected to the fixed shaft 102 via a rotating shaft 103. This design allows the first filter assembly 105 to be easily removed from the opening 104, facilitating cleaning and replacement of the first filter screen 1054. The connection between the rotating shaft 103 and the fixed shaft 102 adopts a snap-fit ​​structure 7. A buffer spring 8 is provided at the bottom of the snap-fit ​​7. When the snap-fit ​​7 is pressed, the rotating shaft 103 can rotate. After the snap-fit ​​7 pops out, it locks into the slot 6, ensuring a stable and reliable connection between the rotating shaft 103 and the fixed shaft 102.

[0030] The bottom end of the inlet pipe 107, connected to the lower end of the inlet port 101, is equipped with a sealing gasket 106. The sealing gasket 106 contacts the first filter assembly 105, preventing liquid leakage from the gap between the first filter assembly 105 and the inlet pipe 107, ensuring that all liquid is filtered by the first filter screen 1054. The first filter assembly 105 and the second filter assembly 201 have similar structures, both adopting a combination design of a ring pipe 1051 and a sleeve 1052. The sleeve 1052 is fixed to the bottom end of the ring pipe 1051 by bolts 1053, and the filter screen is placed inside the sleeve 1052. This design allows the filter screen to be firmly fixed in the filter assembly while facilitating replacement.

[0031] The first filter 1054 in the first filter assembly 105 has a pore size of 70 μm, and the second filter 202 in the second filter assembly 201 has a pore size of 40 μm. These pore sizes are carefully designed to effectively separate cells of different sizes. For example, when processing bone marrow samples, hematopoietic stem cells and progenitor cells of different sizes can be separated; when processing blood samples, different types of blood cells can be separated; and when processing tissue digestion fluids, different types of tissue cells can be separated.

[0032] The second filter box 2 has a collection tube 4 at its bottom, and the lower end of the collection tube 4 is connected to the infusion bag 3. This design allows the liquid after double filtration to be easily collected in the infusion bag 3 for subsequent processing and use. The infusion bag 3 can be a disposable sterile bag or a reusable container, depending on the actual needs.

[0033] The cell dual-layer filtration device of this embodiment is simple to operate and highly efficient. The user simply pours the liquid sample containing cells into the inlet 101. The liquid, under gravity, passes sequentially through the first filter assembly 105 and the second filter assembly 201, and is finally collected in the infusion bag 3. The entire process requires no external power, saving energy and reducing mechanical damage to the cells.

[0034] Furthermore, the cell double-layer filtration device of this embodiment has a compact structure, occupies little space, and is easy to use in laboratories or medical institutions. All components of the device can be manufactured using medical-grade materials to ensure biocompatibility and safety. The first filter 1054 and the second filter 202 can be made of nylon or polyester, materials that have good chemical stability and mechanical strength, and can withstand repeated use and washing.

[0035] In practical applications, the cell double-layer filtration device of this embodiment can be used in a variety of cell separation and purification scenarios. For example, in stem cell research, it can be used to separate stem cells and progenitor cells of different sizes; in immunology research, it can be used to separate different types of immune cells; in tumor research, it can be used to separate tumor cells and normal cells; and in tissue engineering, it can be used to separate and purify cells for constructing tissues.

[0036] The cell double-layer filtration device of this embodiment can also be adjusted and optimized according to actual needs. For example, filters with different pore sizes can be replaced to meet the separation requirements of different types of cells; the size and shape of the first filter box 1 and the second filter box 2 can be adjusted to accommodate samples of different volumes; and the length and diameter of the infusion tube 5 can be adjusted to control the speed of liquid flow.

[0037] In summary, the cell double-layer filtration device provided in this embodiment has a simple structure, is easy to use, can effectively separate cells of different sizes, and has broad application prospects.

[0038] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A cell-based double-layer filtration device, characterized in that, The system includes a first filter box (1) and a second filter box (2), which are connected by an infusion tube (5). A fixed shaft (102) is provided at the side wall connection of the first filter box (1), and an opening (104) is provided through the lower end of the front wall of the first filter box (1). A rotating shaft (103) is movably connected to the fixed shaft (102) at the opening (104), and a first filter assembly (105) is fixedly provided at one end of the rotating shaft. The first filter box (1) has an inlet (101) through the upper end, and the inlet (101) is connected to an inlet pipe (107) fixed on the inner wall of the upper end of the first filter box (1). The inlet pipe (107) has a sealing gasket (106) at the bottom end, and the first filter assembly (105) is movably arranged at the bottom end of the sealing gasket (106).

2. The cell double-layer filtration device according to claim 1, characterized in that: The first filter assembly (105) includes a ring tube (1051), a sleeve (1052), a bolt (1053), and a first filter screen (1054). The sleeve (1052) is disposed inside the ring tube (1051). The bottom of the sleeve (1052) is fixed to the bottom end of the ring tube (1051) by the bolt (1053). The first filter screen (1054) is placed inside the sleeve (1052). The pore size of the first filter screen (1054) is 70μm.

3. The cell double-layer filtration device according to claim 1, characterized in that: The second filter box (2) is also provided with a second filter assembly (201), which includes a ring tube (1051), a sleeve (1052), and a bolt (1053). The second filter assembly (201) also includes a second filter screen (2011), which has a pore size of 40μm.

4. The cell double-layer filtration device according to claim 3, characterized in that: Two sets of buckles (7) are provided at the movable connection between the fixed shaft (102) and the rotating shaft (103). A slot (6) is provided on the rotating shaft (103). A movable groove (9) is provided at the connection between the fixed shaft (102) and the buckle (7). A buffer spring (8) is provided at the bottom of the buckle (7). The bottom of the buffer spring (8) is connected to the inside of the slot (6).

5. A cell double-layer filtration device according to claim 3 or 4, characterized in that: The bottom of the second filter box (2) is provided with a liquid collection tube (4), and the lower end of the liquid collection tube (4) is provided with an infusion bag (3).

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