Soft suction pipette with filtered tissue block

Abstract

The utility model discloses a kind of pipette of soft suction clamp and with filtering tissue block, belong to biomedical equipment field, including pipette tube body, conical filter tube, connecting pipe and trapezoidal soft suction head.Pipette tube body bottom end thread connection conical filter tube, conical filter tube bottom end thread connection connecting pipe, connecting pipe low end bonding trapezoidal soft suction head.Conical filter tube includes filter tube body, conical grid is fixedly bonded inside filter tube body, upper outer thread is set on filter tube body upper end outside, lower inner thread is set on inside lower end.Trapezoidal grid upper end sets up upper filter grid, lower end sets up lower filter grid, wherein lower filter grid gap size is greater than cell tissue block maximum size, upper filter grid gap size is less than cell tissue block minimum size.Pipette tube body side surface is set up observation window, upper end inside sets up piston push rod.Trapezoidal soft suction head adopts medical grade silica gel material.The utility model structure is simple, convenient to operate, can effectively filter and collect cell tissue block, applicable to biomedical field.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical equipment, specifically relating to a pipette with a soft suction clamp and a filter tissue block. Background Technology

[0002] Pipettes are commonly used laboratory instruments in cell culture and tissue block processing, used for the precise aspiration and transfer of liquids and tissue blocks. During cell culture, the culture medium needs to be changed regularly to provide fresh nutrients and remove metabolic waste, and cell or tissue blocks also need to be passaged.

[0003] Traditional pipettes, due to the limitations of their rigid materials and structural design, are prone to causing mechanical damage to cells during these operations, affecting cell viability and culture results.

[0004] First, most pipettes are made of rigid materials, which can easily cause mechanical damage when aspirating and transferring cells or tissue blocks, affecting cell viability and subsequent culture results. Second, existing filtration devices are mostly external attachments, increasing operational complexity and offering limited filtration efficiency, unable to effectively separate tissue blocks of different sizes. Third, existing pipettes often use simple plug-in connections or disposable designs, resulting in poor sealing performance and a tendency for leakage or contamination. Finally, existing pipettes often require additional tools or steps to prevent clogging or separate tissue blocks of different sizes during tissue block processing, increasing the complexity and time cost of experimental procedures.

[0005] Therefore, there is an urgent need for a pipette that can gently aspirate cells and tissue blocks, has a filtering function, a stable connection, and is easy to operate, in order to improve the efficiency and quality of cell culture and tissue block processing. Utility Model Content

[0006] To address the technical problem that traditional pipettes can scratch cells during operation, thus affecting cell culture results, this invention provides a pipette with a soft suction clamp and a filter tissue block.

[0007] The technical solution adopted by this utility model to solve its technical problem is as follows: a pipette with a soft suction clamp and a filter tissue block is provided, including a pipette body, a conical filter tube threaded to the bottom end of the pipette body, a connecting tube threaded to the bottom end of the conical filter tube, and a trapezoidal soft suction tip bonded to the lower end of the connecting tube; the conical filter tube includes a filter tube body, a conical grid is bonded and fixed inside the filter tube body, an upper external thread is provided on the outer side of the upper end of the filter tube body, a lower internal thread is provided on the lower end of the inner side of the filter tube body, an upper filter grid is provided on the upper end of the conical grid, and a lower filter grid is provided on the lower end of the conical grid.

[0008] Preferably, the inner side of the bottom end of the pipette body is provided with an upper internal thread, and the outer side of the upper end of the connecting tube is provided with a lower external thread. Teflon tape is provided at the connection between the upper internal thread and the upper external thread, and at the connection between the lower external thread and the lower internal thread.

[0009] Furthermore, the trapezoidal soft suction tip is made of medical-grade silicone.

[0010] Furthermore, the lower filter grid has a pore size larger than the maximum size of the cell tissue block, while the upper filter grid has a pore size smaller than the minimum size of the cell tissue block.

[0011] Furthermore, an observation window is provided on the side of the pipette body.

[0012] Furthermore, a piston push rod is provided on the inner side of the upper end of the pipette body.

[0013] The beneficial effects of this utility model are as follows:

[0014] 1. By designing a trapezoidal soft pipette tip below the pipette and using medical-grade silicone material, direct contact with the cell layer is avoided, reducing scratch damage to the bottom of the culture flask and effectively protecting the cell integrity during the cell culture process;

[0015] 2. By designing a conical grid structure inside the pipette, in which the gap size of the upper filter grid is smaller than the minimum size of the cell tissue block and the gap size of the lower filter grid is larger than the maximum size of the cell tissue block, the unidirectional passage function of the tissue block is realized, that is, the tissue block can be drawn into the pipette but is intercepted inside the pipette when blown out;

[0016] 3. Through a special structural design, the tissue block can be compressed and deformed to pass through the tip during aspiration, but is wedged into the narrow opening and cannot pass through during expulsion, effectively controlling the flow direction of the tissue block and improving the accuracy and success rate of cell culture operations;

[0017] 4. The observation window on the side of the pipette body allows the operator to observe the state of the liquid and tissue block in real time during the pipetting process, improving the accuracy of the operation;

[0018] 5. The threaded connection structure combined with the Teflon tape design ensures the sealing and stability of the connections between components, prevents liquid leakage, and facilitates disassembly and cleaning, thereby improving the service life and hygiene of the equipment. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is an exploded structural diagram of the present invention;

[0021] Figure 3This is a schematic diagram of the connection structure between the conical filter tube and the connecting tube of this utility model;

[0022] Figure 4 This is a schematic diagram of the internal structure of the conical filter tube of this utility model;

[0023] Figure 5 This is a schematic diagram of the internal structure of the conical filter tube and conical grid of this utility model.

[0024] Legend:

[0025] 1-Piston rod, 2-Pipette body, 201-Upper internal thread, 3-Connecting tube, 301-Lower external thread, 4-Trapezoidal soft pipette tip, 5-Conical filter tube, 501-Upper external thread, 502-Filter tube body, 503-Lower internal thread, 504-Conical grid, 5041-Upper filter grid, 5042-Lower filter grid, 6-Observation window. Detailed Implementation

[0026] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings and embodiments. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0027] Example 1

[0028] like Figure 1 As shown, a pipette with a soft suction clamp and a filter tissue block includes a pipette body 2, a conical filter tube 5 threaded to the bottom end of the pipette body 2, a connecting tube 3 threaded to the bottom end of the conical filter tube 5, and a trapezoidal soft suction tip 4 bonded to the lower end of the connecting tube 3.

[0029] like Figure 4 As shown, the conical filter tube 5 includes a filter tube body 502, and a conical grid 504 is bonded and fixed inside the filter tube body 502. An upper external thread 501 is provided on the outer side of the upper end of the filter tube body 502, and a lower internal thread 503 is provided on the lower end of the inner side of the filter tube body 502. An upper filter grid 5041 is provided on the upper end of the conical grid 504, and a lower filter grid 5042 is provided on the lower end of the conical grid 504.

[0030] like Figure 2 and Figure 3As shown, the inner side of the bottom end of the pipette body 2 is provided with an upper internal thread 201, and the outer side of the upper end of the connecting tube 3 is provided with a lower external thread 301. PTFE tape is provided at the connection points of the upper internal thread 201 and the upper external thread 501, and at the connection points of the lower external thread 301 and the lower internal thread 503. The PTFE tape effectively prevents liquid leakage, ensures sealing performance during pipetting, improves the reliability of the pipette, and facilitates disassembly and cleaning.

[0031] The trapezoidal soft suction tip 4 is made of medical-grade silicone. Medical-grade silicone has good biocompatibility and elasticity, can adapt to tissue surfaces of different shapes, provides gentle adsorption force, reduces mechanical damage to cells and tissues, and also has good high-temperature resistance, allowing for high-temperature sterilization to ensure the aseptic requirements of experimental operations.

[0032] like Figure 5 As shown, the pore size of the lower filter grid 5042 is larger than the maximum size of the cell tissue block, while the pore size of the upper filter grid 5041 is smaller than the minimum size of the cell tissue block. This design allows the cell tissue block to pass through the lower filter grid 5042 into the conical grid 504, but it cannot pass through the upper filter grid 5041 and is thus trapped inside the conical grid 504. This dual-layer filtration structure can effectively collect and filter the cell tissue block, preventing it from being drawn into the pipette body 2 and avoiding contamination or interference with experimental results.

[0033] like Figure 1 As shown, an observation window 6 is provided on the side of the pipette body 2. The observation window 6 allows the operator to directly observe the state of the liquid and cell tissue block inside the pipette, making it easier to judge the amount and quality of the sample taken, and improving the accuracy and efficiency of the experimental operation.

[0034] A piston rod 1 is installed on the inner side of the upper end of the pipette body 2. The piston rod 1 changes the air pressure inside the pipette by pushing and pulling, thereby realizing the functions of liquid aspiration and discharge. When the piston rod 1 is pulled upward, a negative pressure is formed inside the pipette, and liquid and cell tissue blocks are aspirated through the trapezoidal soft pipette tip 4; when the piston rod 1 is pushed downward, a positive pressure is formed inside the pipette, and liquid is discharged, while cell tissue blocks are intercepted by the conical grid 504.

[0035] During use, the operator first gently places the trapezoidal soft pipette tip 4 close to the tissue block to be aspirated, then slowly pulls the piston rod 1, using negative pressure to draw the tissue block and surrounding liquid into the pipette. Because the trapezoidal soft pipette tip 4 is made of medical-grade silicone, it gently conforms to the tissue surface, reducing mechanical damage to the cells. After the tissue block enters the conical filter tube 5 with the liquid, it passes through the lower filter grid 5042 and enters the conical grid 504, but is blocked by the upper filter grid 5041, thus being retained inside the conical grid 504. The operator can observe the state and position of the tissue block through the observation window 6 to ensure the accuracy of the experimental operation. When it is necessary to release the tissue block, simply align the pipette with the target container and gently push the piston rod 1 to release the tissue block and liquid together to the target location.

[0036] This soft-clamp pipette with a built-in filter for tissue blocks has a simple structure and is easy to operate. It can effectively aspirate, filter, and transfer cell tissue blocks, reduce mechanical damage to cells, and improve the success rate and accuracy of cell experiments. It is particularly suitable for biomedical research fields such as cell culture and tissue engineering.

[0037] The above are all preferred embodiments of this application and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A pipette with a soft suction clamp and a filter tissue block, comprising a pipette body (2), characterized in that, The bottom end of the pipette body (2) is threaded with a conical filter tube (5), the bottom end of the conical filter tube (5) is threaded with a connecting tube (3), and a trapezoidal soft suction tip (4) is bonded to the bottom end of the connecting tube (3); the conical filter tube (5) includes a filter tube body (502), a conical grid (504) is bonded and fixed inside the filter tube body (502), an upper external thread (501) is provided on the outer side of the upper end of the filter tube body (502), a lower internal thread (503) is provided on the lower end of the inner side of the filter tube body (502), an upper filter grid (5041) is provided on the upper end of the conical grid (504), and a lower filter grid (5042) is provided on the lower end of the conical grid (504).

2. The pipette with a soft suction clamp and a filter tissue block according to claim 1, characterized in that: The inner side of the bottom end of the pipette body (2) is provided with an upper internal thread (201), and the outer side of the upper end of the connecting tube (3) is provided with a lower external thread (301). The connection between the upper internal thread (201) and the upper external thread (501) and the connection between the lower external thread (301) and the lower internal thread (503) are provided with raw material tape.

3. The pipette with a soft suction clamp and a filter tissue block according to claim 1, characterized in that: The trapezoidal soft suction head (4) is made of medical-grade silicone.

4. The pipette with a soft suction clamp and a filter tissue block according to claim 1, characterized in that: The lower filter grid (5042) has a pore size larger than the maximum size of the cell tissue block, while the upper filter grid (5041) has a pore size smaller than the minimum size of the cell tissue block.

5. A pipette with a soft suction clamp and a filter tissue block according to claim 1, characterized in that: An observation window (6) is provided on the side of the pipette body (2).

6. A pipette with a soft suction clamp and a filter tissue block according to claim 1, characterized in that: A piston rod (1) is provided on the inner side of the upper end of the pipette body (2).

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