A centrifugal fixing device for solid phase extraction cartridges

Abstract

The utility model belongs to the technical field of auxiliary centrifugal device, concretely relates to a centrifugal fixing device of solid phase extraction column, including centrifugal tube, the top of centrifugal tube is provided with centrifugal tube cover, and the threaded connection between centrifugal tube cover and centrifugal tube, the lower end of centrifugal tube is provided with centrifugal tube bottom, and the threaded connection between centrifugal tube bottom and centrifugal tube, the inside of centrifugal tube bottom is provided with third annular clamping slot, the inside of centrifugal tube is provided with first annular clamping slot and second annular clamping slot, the three -segmented screw thread connection structure of centrifugal tube cover, centrifugal tube and centrifugal tube bottom in the device, the sealing of device is ensured, can prevent the invasion of dust and bacteria in the centrifugal process, avoid the pollution of solid phase extraction column in the blow -dry process, and the first annular clamping slot and second annular clamping slot set up in centrifugal tube inside, can fix solid phase extraction column, make it keep the state of suspension in the centrifugal process, improve the stability of centrifugal, reduce the artificial error caused by the uneven blow -dry of traditional ear cleaning ball.

Description

Technical Field

[0001] This utility model belongs to the technical field of auxiliary centrifugation devices, specifically relating to a centrifugation fixing device for a solid phase extraction column. Background Technology

[0002] Solid phase extraction (SPE), as a highly efficient sample pretreatment technique, is widely used in chemical analysis, environmental monitoring, biomedicine, and food safety, among other fields. This technique combines the high selectivity of liquid-solid extraction columns with the efficient separation capabilities of liquid chromatography, primarily for sample separation, purification, and concentration. Compared to traditional liquid-liquid extraction, SPE significantly improves analyte recovery and more effectively separates target analytes from interfering substances in the sample, thus simplifying sample pretreatment steps and achieving ease of operation, time-saving, and labor-saving results.

[0003] However, a crucial step in the final stage of the solid-phase extraction (SPE) process is to thoroughly dry the SPE column to ensure smooth subsequent elution. Currently, the commonly used laboratory methods for drying SPE columns rely on manual tools such as rubber bulbs to apply positive pressure to the column, thereby draining and drying the liquid inside. However, this method has significant limitations: 1. Risk of contamination: If there are impurities inside the syringe bulb, these impurities can easily be carried into the solid phase extraction column during the blowing process, causing sample contamination and affecting the accuracy of the analysis results.

[0004] 2. Inconsistent Operation: Due to the uncertainty of human operation, different operators or the same operator at different times may apply different blowing forces to the solid-phase extraction column, resulting in uneven drying of the column. This inconsistency not only increases experimental error but may also affect the sensitivity and reproducibility of subsequent analyses.

[0005] 3. Low efficiency: The manual drying process is time-consuming, and operators are prone to fatigue when processing large batches of samples, which further reduces work efficiency. Utility Model Content

[0006] To address the aforementioned shortcomings of existing technologies, this invention provides a centrifugal fixing device for solid phase extraction columns, which solves the problems of easy contamination and low efficiency of manual operation during the drying process of existing solid phase extraction columns.

[0007] To achieve the above objectives, the technical solution adopted by this utility model is as follows: A centrifugal fixing device for a solid-phase extraction column is provided, including a centrifuge tube, one end of which is provided with a centrifuge tube cap, and the centrifuge tube cap is threadedly connected to the centrifuge tube; the other end of the centrifuge tube is provided with a centrifuge tube bottom, and the centrifuge tube bottom is threadedly connected to the centrifuge tube; a third annular groove is provided inside the centrifuge tube bottom; a first annular groove and a second annular groove are provided inside the centrifuge tube for fixing the solid-phase extraction column.

[0008] The beneficial effects of adopting the above technical solution are as follows: the centrifugation fixing device of the solid phase extraction column adopts centrifugation instead of the traditional ear washing bulb drying method, which not only avoids the contamination of the solid phase extraction column by impurities in the ear washing bulb, but also ensures the uniformity of force on each solid phase extraction column through uniform centrifugation, thereby reducing experimental errors and improving experimental efficiency and accuracy. The centrifuge tube cap is threaded to the centrifuge tube, ensuring the tube's airtightness during centrifugation. This sealed environment effectively prevents external contaminants such as dust and bacteria from entering the tube, thus avoiding contamination of the solid-phase extraction (SPE) column. The threaded connection also allows operators to quickly and safely open and close the centrifuge tube, improving the convenience and safety of experimental operations. The first and second annular grooves inside the centrifuge tube are used to fix the SPE column, ensuring its stability during centrifugation and keeping it suspended. This promotes uniform distribution and rapid drainage of the liquid within the SPE column, improving drying efficiency. The design of the first and second annular grooves also facilitates quick and accurate installation and removal of the SPE column. Furthermore, the centrifuge tube bottom is also threaded to the centrifuge tube, allowing operators to easily disassemble and clean the bottom as needed. A third annular groove inside the centrifuge tube bottom is used to fix the collection tube, facilitating the collection of liquid detached from the SPE column during centrifugation. This not only improves the efficiency and accuracy of liquid collection but also facilitates subsequent sample processing and analysis.

[0009] Furthermore, the centrifuge tube cap has a first threaded interface on its inner circumference, and one end of the centrifuge tube has a second threaded interface that is threadedly connected to the first threaded interface on its outer side; the other end of the centrifuge tube has a third threaded interface on its outer side, and the bottom of the centrifuge tube has a fourth threaded interface that is threadedly connected to the third threaded interface on its inner circumference.

[0010] The beneficial effects of adopting the above technical solution are as follows: the centrifuge tube cap and the centrifuge tube are connected through the first threaded interface and the second threaded interface, and the centrifuge tube and the bottom of the centrifuge tube are connected through the third threaded interface and the fourth threaded interface. This threaded connection method can not only ensure the connection stability and sealing between the centrifuge tube cap, centrifuge tube and the bottom of the centrifuge tube, but also prevent external contaminants such as dust and bacteria from entering the centrifuge tube and contaminating the solid phase extraction column. At the same time, it ensures the airtightness of the device during centrifugation, avoids experimental errors caused by improper human operation or poor device sealing, thereby improving the accuracy and reliability of the experiment.

[0011] Furthermore, the first annular groove is located below the second threaded interface, and the inner diameter of the first annular groove is larger than the outer diameter of the solid phase extraction column.

[0012] Furthermore, the second annular groove is located below the first annular groove, and the inner diameter of the second annular groove is larger than the outer diameter of the bottom of the solid phase extraction column.

[0013] The beneficial effects of adopting the above technical solution are as follows: A certain distance is set between the first and second annular grooves, forming a stable double-point support structure for the solid-phase extraction column. This ensures the vertical suspension of the solid-phase extraction column during centrifugation, reducing cross-contamination with the centrifuge tube wall. Furthermore, the inner diameter of the first annular groove is greater than the outer diameter of the solid-phase extraction column body, and the inner diameter of the second annular groove is greater than the outer diameter of the bottom of the solid-phase extraction column body. This ensures the stable fixation of the solid-phase extraction column body and its bottom, avoiding shaking or displacement during centrifugation, thereby effectively reducing human error. It also improves the drying efficiency of the solid-phase extraction column and the accuracy of experimental results.

[0014] Furthermore, the bottom of the centrifuge tube is a cone structure with the tip pointing vertically downwards, and the taper of the bottom of the centrifuge tube is 20°.

[0015] The beneficial effects of adopting the above technical solution are as follows: the bottom of the centrifuge tube is a conical structure with the tip pointing vertically downwards, and the taper of the bottom of the centrifuge tube is 20°. This can effectively guide the liquid that is removed from the solid-phase extraction column during centrifugation to flow along the tube wall of the bottom of the centrifuge tube to the lowest point of the bottom of the centrifuge tube, which facilitates complete collection of liquid and avoids residue. At the same time, it reduces the risk of secondary contact between liquid and solid-phase extraction column, thereby reducing cross-contamination. In addition, it is also convenient to collect liquid after disassembling the bottom of the centrifuge tube, which improves the convenience of experimental operation and the efficiency of liquid recovery.

[0016] In summary, the centrifugal fixation device for solid-phase extraction columns provided by this utility model has the following beneficial effects: This device employs a three-section threaded connection structure consisting of the centrifuge tube cap, centrifuge tube, and centrifuge tube bottom. This facilitates disassembly while ensuring the device's airtightness, preventing the intrusion of dust and bacteria during centrifugation and avoiding contamination of the solid-phase extraction column during the drying process. Furthermore, the first and second annular grooves inside the centrifuge tube securely fix the solid-phase extraction column, keeping it suspended during centrifugation. This not only improves centrifugation stability but also reduces human error caused by uneven drying with traditional syringes. In addition, when centrifugation demand increases, multiple centrifuge tubes of the same specifications can be simultaneously placed into the centrifuge for batch and standardized processing, significantly shortening pretreatment time. It also maintains consistency in centrifugation conditions during batch centrifugation, ensuring the accuracy and repeatability of experimental results and greatly improving experimental efficiency and quality. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model; Among them, 1. centrifuge tube cap; 2. centrifuge tube; 3. centrifuge tube bottom; 4. first threaded interface; 5. second threaded interface; 6. first annular groove; 7. second annular groove; 8. third threaded interface; 9. fourth threaded interface; 10. third annular groove. Detailed Implementation

[0018] The specific embodiments of this utility model are described below to enable those skilled in the art to understand this utility model. However, it should be understood that this utility model is not limited to the scope of the specific embodiments. For those skilled in the art, as long as various changes are within the spirit and scope of this utility model as defined and determined by the appended claims, these changes are obvious. All utility model creations utilizing the concept of this utility model are within the scope of protection.

[0019] like Figure 1As shown, the centrifugal fixing device for solid-phase extraction columns provided by this utility model includes a centrifuge tube 2, one end of which is provided with a centrifuge tube cap 1, and the centrifuge tube cap 1 is threadedly connected to the centrifuge tube 2; the other end of the centrifuge tube 2 is provided with a centrifuge tube bottom 3, and the centrifuge tube bottom 3 is threadedly connected to the centrifuge tube 2; a third annular groove 10 is provided on the inner circumference of the centrifuge tube bottom 3; a first annular groove 6 and a second annular groove 7 are provided inside the centrifuge tube 2 for fixing the solid-phase extraction column. The centrifuge tube cap 1 and centrifuge tube 2 are connected by a threaded connection, which ensures the airtightness of centrifuge tube 2 during centrifugation. The airtight environment can effectively prevent external contaminants such as dust and bacteria from entering the centrifuge tube 2, thereby avoiding contamination of the solid phase extraction column. At the same time, the threaded connection also makes it easy for operators to quickly and safely open and close centrifuge tube 2, improving the convenience and safety of experimental operations. The first annular groove 6 and the second annular groove 7 set inside centrifuge tube 2 are used to fix the solid phase extraction column. This not only ensures the stability of the solid phase extraction column during centrifugation, but also allows the solid phase extraction column to be suspended, thereby promoting the uniform distribution and rapid discharge of liquid in the solid phase extraction column and improving the drying efficiency. At the same time, the design of the first annular groove 6 and the second annular groove 7 makes it easy for operators to quickly and accurately install and remove the solid phase extraction column. The centrifuge tube bottom 3 and centrifuge tube 2 are also connected by threads, which makes it easy for operators to disassemble and clean the centrifuge tube bottom 3 as needed. The centrifuge tube bottom 3 is provided with a third annular groove 10 with a diameter of 13mm. The third annular groove 10 is used to fix a 1.5ml collection tube. The inner diameter of the collection tube is smaller than the inner diameter of the third annular groove 10. The collection tube facilitates the collection of liquid that falls off the solid phase extraction column during centrifugation, which not only improves the efficiency and accuracy of liquid collection, but also facilitates subsequent sample processing and analysis.

[0020] In this invention, the centrifuge tube cap 1, centrifuge tube 2, and centrifuge tube bottom 3 are all made of polypropylene. Polypropylene has excellent chemical stability, corrosion resistance, and thermal stability, and can effectively resist various organic solvents and acid and alkali reagents that may come into contact with it during the experiment, avoiding chemical reactions between the material and the solid phase extraction column that could lead to contamination. At the same time, the surface of polypropylene is smooth and does not easily adsorb impurities, further reducing the risk of cross-contamination.

[0021] like Figure 1As shown, the centrifuge tube cap 1 has a first threaded interface 4 on its inner circumference, and a second threaded interface 5 that is threadedly connected to the first threaded interface 4 is provided on the outer side of one end of the centrifuge tube 2; a third threaded interface 8 is provided on the outer side of the other end of the centrifuge tube 2, and a fourth threaded interface 9 that is threadedly connected to the third threaded interface 8 is provided on the inner circumference of the centrifuge tube bottom 3. The centrifuge tube cap 1 and the centrifuge tube 2 are connected through the first threaded interface 4 and the second threaded interface 5, and the centrifuge tube 2 and the centrifuge tube bottom 3 are connected through the third threaded interface 8 and the fourth threaded interface 9. This threaded connection method can ensure the stability and sealing of the connection between the centrifuge tube cap 1, the centrifuge tube 2 and the centrifuge tube bottom 3, and prevent external contaminants such as dust and bacteria from entering the centrifuge tube 2 and contaminating the solid phase extraction column. At the same time, it can ensure the airtightness of the device during centrifugation, avoid experimental errors caused by improper human operation or poor sealing of the device, thereby improving the accuracy and reliability of the experiment.

[0022] like Figure 1 As shown, the first annular groove 6 is located 0.5 cm below the second threaded interface 5. The inner diameter of the first annular groove 6 is 4.4 mm, and it is larger than the outer diameter of the solid-phase extraction column. The second annular groove 7 is located 5 cm below the first annular groove 6. The inner diameter of the second annular groove 7 is 1.1 cm, and it is larger than the outer diameter of the bottom of the solid-phase extraction column. A certain distance is provided between the first annular groove 6 and the second annular groove 7, forming a stable double-point support structure for the solid-phase extraction column. This ensures the vertical suspension of the solid-phase extraction column during centrifugation, reducing cross-contamination with the centrifuge tube 2 wall. Furthermore, the inner diameter of the first annular groove 6 is larger than the outer diameter of the solid-phase extraction column, and the inner diameter of the second annular groove 7 is larger than the outer diameter of the bottom of the solid-phase extraction column, ensuring the stable fixation of the solid-phase extraction column and its bottom, avoiding shaking or displacement during centrifugation, and effectively reducing human error. This improved the drying efficiency of solid-phase extraction columns and the accuracy of experimental results.

[0023] like Figure 1 As shown, the centrifuge tube bottom 3 is a cone structure with its tip pointing vertically downwards, and the taper of the centrifuge tube bottom 3 is 20°. The cone structure of the centrifuge tube bottom 3, with its tip pointing vertically downwards and a taper of 20°, effectively guides the liquid that has detached from the solid-phase extraction column during centrifugation along the tube wall to the lowest point of the centrifuge tube bottom 3. This facilitates complete liquid collection and avoids residue, while also reducing the risk of secondary contact between the liquid and the solid-phase extraction column, thereby reducing cross-contamination. Furthermore, it facilitates the collection of liquid after disassembling the centrifuge tube bottom 3, improving the convenience of experimental operations and the efficiency of liquid recovery.

[0024] The working method of this utility model is as follows: First, the centrifuge tube bottom 3 and centrifuge tube 2 are fixedly connected by the third threaded interface 8 and the fourth threaded interface 9 to ensure that there is no leakage at the connection. Then, the solid phase extraction column is inserted from the top of the centrifuge tube 2, so that it passes through the first annular groove 6 and the second annular groove 7 in the centrifuge tube 2 in sequence to fix the solid phase extraction column and prevent it from moving or falling off during centrifugation. After the solid phase extraction column is fixed, the centrifuge tube cap 1 is covered and fixed to the centrifuge tube 2 by the first threaded interface 4 and the second threaded interface 5 to form a sealed environment to prevent dust and bacteria from entering. Finally, the centrifuge tube 2 with the solid phase extraction column fixed is placed in the centrifuge. The appropriate centrifuge speed is set according to the adsorption capacity of the extracted sample in the solid phase extraction column, and uniform centrifugation is performed. When the sample volume is large, it can be centrifuged in batches under the same centrifugation conditions. Through centrifugation, the solid phase extraction column can be dried at the same time, which not only reduces human error caused by uneven drying, but also significantly saves operation time and improves experimental efficiency.

[0025] In summary, the centrifugation fixing device for solid-phase extraction columns provided by this utility model, through a three-section threaded connection structure of centrifuge tube cap 1, centrifuge tube 2, and centrifuge tube bottom 3, facilitates disassembly while ensuring the device's airtightness, preventing the intrusion of dust and bacteria during centrifugation and avoiding contamination of the solid-phase extraction column during the drying process. Furthermore, the first annular groove 6 and the second annular groove 7 inside the centrifuge tube 2 securely fix the solid-phase extraction column, keeping it suspended during centrifugation. This not only improves centrifugation stability but also reduces human error caused by uneven drying with traditional wash bulbs. In addition, when the demand for centrifuged samples increases, multiple centrifuge tubes 2 of the same specification can be simultaneously placed into the centrifuge to achieve batch and standardized processing, significantly shortening pretreatment time. Simultaneously, it maintains consistency in centrifugation conditions during batch centrifugation, thereby ensuring the accuracy and repeatability of experimental results and greatly improving experimental efficiency and quality.

Claims

1. A centrifugal fixation device for a solid-phase extraction column, characterized in that: The centrifuge tube (2) is provided with a centrifuge tube cap (1) at one end, and the centrifuge tube cap (1) is threadedly connected to the centrifuge tube (2); the centrifuge tube bottom (3) is provided at the other end, and the centrifuge tube bottom (3) is threadedly connected to the centrifuge tube (2); a third annular groove (10) is provided inside the centrifuge tube bottom (3); a first annular groove (6) and a second annular groove (7) are provided inside the centrifuge tube (2) for fixing the solid phase extraction column.

2. The centrifugal fixation device for solid-phase extraction columns according to claim 1, characterized in that: The centrifuge tube cap (1) has a first threaded interface (4) on its inner circumference. The centrifuge tube (2) has a second threaded interface (5) on its outer side that is threaded to the first threaded interface (4). The centrifuge tube (2) has a third threaded interface (8) on its outer side at the other end. The centrifuge tube bottom (3) has a fourth threaded interface (9) on its inner circumference that is threaded to the third threaded interface (8).

3. The centrifugal fixation device for solid-phase extraction columns according to claim 1, characterized in that: The first annular groove (6) is located below the second threaded interface (5), and the inner diameter of the first annular groove (6) is larger than the outer diameter of the solid phase extraction column.

4. The centrifugal fixation device for solid-phase extraction columns according to claim 1, characterized in that: The second annular groove (7) is located below the first annular groove (6), and the inner diameter of the second annular groove (7) is larger than the outer diameter of the bottom of the solid phase extraction column.

5. The centrifugal fixation device for solid-phase extraction columns according to claim 1, characterized in that: The centrifuge tube bottom (3) is a cone structure with the tip pointing vertically downwards, and the taper of the centrifuge tube bottom (3) is 20°.

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