A high-throughput cryogenic centrifugation aliquoting apparatus for plasma samples

Abstract

The utility model discloses a kind of high-throughput low-temperature centrifugal sub-packaging equipment for plasma sample, including box, the middle part of box top wall is provided with mounting groove, the front side of box upper end is hinged with box door, the upper and lower position of box door and mounting groove corresponds, the upper side of box inside is equipped with storage box, the upper end of storage box is respectively provided with placing groove, the rear side of box inside is equipped with air cooler, further including fixing mechanism;Fixing mechanism: it includes fixed rod, vertical rod and circle center wheel, vertical rod is rotatably connected in the middle part of the bottom wall of storage box, the upper side of vertical rod outer arc surface is provided with circle center wheel, the upper side in storage box is respectively equipped with fixed rod, the lower end of five fixed rods is respectively located in the adjusting groove in the upper end of vertically adjacent circle center wheel. The high-throughput low-temperature centrifugal sub-packaging equipment for plasma sample, the protection mode is optimized, further increase the stability of plasma sample when rotating, reduce the complexity of operation, improve the separation efficiency of blood sample.

Description

Technical Field

[0001] This utility model relates to the field of pharmacokinetic experimental technology, specifically a high-throughput low-temperature centrifugation and dispensing device for plasma samples. Background Technology

[0002] In pharmacokinetic and bioanalytical experiments, high-throughput cryogenic centrifugation and dispensing equipment for plasma samples can efficiently and accurately process large quantities of plasma samples, which can significantly improve experimental efficiency, shorten experimental cycles, and ensure that the samples are kept at low temperatures throughout the processing to maintain their biological activity and stability.

[0003] In the prior art, patent publication number CN 222000293 U discloses a centrifuge device for sample testing of sepsis patients. During operation, to prevent test tubes from being thrown out of the receiving slot, a pressing component moves the receiving slot downwards, thus retracting the test tubes into the rotating disk. At the end of the operation, the pressing component moves upwards to reset, and then a spring moves the test tube upwards through the receiving slot to reset it, allowing it to extend from the rotating disk. However, after prolonged use, the spring fatigues, reducing its elasticity. This reduced elasticity prevents the test tubes from extending from the rotating disk, requiring the operator to use external tools to remove them, further increasing operational complexity and reducing work efficiency. Utility Model Content

[0004] The technical problem to be solved by this utility model is to overcome the existing defects and provide a high-throughput low-temperature centrifugation and dispensing device for plasma samples. It can limit and fix the test tube containing the plasma sample, optimize the protection method, and further increase the stability of the plasma sample during rotation. In the subsequent removal process, there is no need for a spring to push it out, which reduces the complexity of operation and improves the separation efficiency of blood samples. It can effectively solve the problems in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a high-throughput low-temperature centrifugation and dispensing device for plasma samples, comprising a box body, an installation groove provided in the middle of the top wall of the box body, a door hinged to the front side of the upper end of the box body, the door corresponding to the upper and lower positions of the installation groove, a storage box provided on the upper side of the inside of the box body, a placement groove provided on the upper end of the storage box, a cold air fan provided on the rear side of the inside of the box body, and a fixing mechanism.

[0006] The fixing mechanism includes a fixing rod, a vertical rod, and a central wheel. The vertical rod is rotatably connected to the middle of the bottom wall of the storage box. A central wheel is provided on the upper side of the outer arc surface of the vertical rod. Fixing rods are respectively provided on the upper side of the inside of the storage box. The lower ends of the five fixing rods are respectively located in the adjustment grooves opened at the upper ends of the vertically adjacent central wheels.

[0007] Furthermore, it also includes a controller, which is located at the top of the enclosure. The input terminal of the controller is electrically connected to an external power source, and the input terminal of the air cooler is electrically connected to the output terminal of the controller, enabling the regulation of the electrical components inside the equipment.

[0008] Furthermore, the fixing mechanism also includes a hexagonal sliding column and a top plate. The hexagonal sliding column is slidably connected to the upper side of the arc surface of the placement groove. The end of the hexagonal sliding column away from the center of the inside of the storage box is provided with a top plate. The lower end of the hexagonal sliding column near the center of the inside of the storage box is fixedly connected with a fixing rod, which can fix the test tube containing the plasma sample.

[0009] Furthermore, the fixing mechanism also includes a worm gear, a mounting rod, a worm, and an adjustment knob. The worm gear is located on the lower side of the outer arc surface of the upright. The mounting rod is rotatably connected to the middle of the front wall of the storage box. A worm is located on the rear side of the outer arc surface of the mounting rod. The worm gear and the worm are meshed together. An adjustment knob is located at the front end of the mounting rod, which can drive the central wheel to rotate.

[0010] Furthermore, a fixing plate is provided on the lower side of the box body, and a support base is rotatably connected to the middle of the upper part of the fixing plate. A storage box is provided on the upper end of the support base, and a servo motor is provided in the middle of the bottom wall of the box body. The upper end of the output shaft of the servo motor is fixedly connected to the lower end of the support base, and the input end of the servo motor is electrically connected to the output end of the controller, which can drive the storage box to rotate.

[0011] Furthermore, a temperature detector is installed on the front side of the bottom wall of the enclosure. The temperature detector is bidirectionally electrically connected to the controller and monitors the temperature inside the enclosure in real time.

[0012] Furthermore, a connecting groove is provided in the middle of the rear wall of the box, a filter screen is provided on the rear side inside the connecting groove, a cold air fan is fixedly connected to the front side of the bottom wall of the connecting groove, and exhaust holes are provided on the front sides of the left and right ends of the box, which prevents external debris from entering the box and can also quickly exhaust the air inside the box, further improving the air flow speed inside the box.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] It can limit and fix the test tube containing the plasma sample, optimize the protection method, and further increase the stability of the plasma sample during rotation. It does not require a spring to push it out during the subsequent removal process, reducing the complexity of operation and improving the separation efficiency of blood samples. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0016] Figure 2 This is a schematic diagram of the front sectional structure of the present invention;

[0017] Figure 3 This is a schematic cross-sectional view of the right side of this utility model;

[0018] Figure 4 This is an enlarged structural diagram of point A in this utility model.

[0019] In the diagram: 1. Cabinet, 2. Controller, 3. Mounting slot, 4. Cabinet door, 5. Fixing plate, 6. Support base, 7. Storage box, 8. Placement slot, 9. Fixing mechanism, 91. Hexagonal sliding column, 92. Top plate, 93. Fixing rod, 94. Upright rod, 95. Center wheel, 96. Worm gear, 97. Mounting rod, 98. Worm, 99. Adjustment knob, 10. Connecting slot, 11. Filter screen, 12. Air cooler, 13. Exhaust port, 14. Temperature sensor, 15. Servo motor. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Please see Figure 1-4 This embodiment provides a technical solution: a high-throughput low-temperature centrifugation and dispensing device for plasma samples, including a box body 1, an installation groove 3 in the middle of the top wall of the box body 1, a door 4 hinged to the front of the upper end of the box body 1, the door 4 corresponding to the upper and lower positions of the installation groove 3, a storage box 7 in the upper part of the box body 1, a placement groove 8 in the upper part of the storage box 7, and a cold air fan 12 in the rear of the box body 1.

[0022] It also includes a fixing mechanism 9; the fixing mechanism 9 includes a fixing rod 93, a vertical rod 94, and a central wheel 95. The vertical rod 94 is rotatably connected to the middle of the bottom wall of the storage box 7. A central wheel 95 is provided on the upper side of the outer arc surface of the vertical rod 94. The upper side of the inside of the storage box 7 is provided with fixing rods 93 respectively. The lower ends of the five fixing rods 93 are respectively located in the adjustment grooves opened at the upper ends of the vertically adjacent central wheels 95. The fixing mechanism 9 also includes a hexagonal sliding column 91 and a top plate 92. The hexagonal sliding column 91 is slidably connected to the sliding grooves opened on the upper side of the inner arc surface of the placement groove 8. A top plate 92 is provided at the end of the hexagonal sliding column 91 away from the center of the inside of the storage box 7. The lower end of the hexagonal sliding column 91 is close to the inside of the storage box 7. A fixing rod 93 is fixedly connected to one side of the center. The fixing mechanism 9 also includes a worm gear 96, a mounting rod 97, a worm 98, and an adjusting knob 99. The worm gear 96 is located on the lower side of the outer arc surface of the upright rod 94. The mounting rod 97 is rotatably connected to the middle of the front wall of the storage box 7. The worm 98 is located on the rear side of the outer arc surface of the mounting rod 97. The worm gear 96 and the worm 98 are meshed together. The adjusting knob 99 is located at the front end of the mounting rod 97, which can limit and fix the test tube containing the plasma sample, further improving the stability of the plasma sample during rotation. In the subsequent removal process, there is no need for a spring to push it out, reducing the complexity of the operation and increasing the separation efficiency of the blood sample.

[0023] It also includes a controller 2, which is located at the top of the housing 1. The input end of the controller 2 is electrically connected to an external power source, and the input end of the air cooler 12 is electrically connected to the output end of the controller 2, which can regulate the electrical components inside the equipment.

[0024] The box 1 has a fixed plate 5 on its lower side, and a support base 6 is rotatably connected to the middle of the upper end of the fixed plate 5. A storage box 7 is set on the upper end of the support base 6. A servo motor 15 is set on the middle of the bottom wall of the box 1. The upper end of the output shaft of the servo motor 15 is fixedly connected to the lower end of the support base 6. The input end of the servo motor 15 is electrically connected to the output end of the controller 2. The servo motor 15 starts to run through the control of the controller 2. The output shaft of the servo motor 15 drives the support base 6 to rotate. During the rotation of the support base 6, the storage box 7 drives the test tube containing the plasma sample to rotate. During the rotation of the test tube containing the plasma sample, the centrifugal force generated by the high-speed rotation separates the different components (such as blood cells, plasma, etc.) in the plasma sample.

[0025] Among them: a temperature detector 14 is installed on the front side of the bottom wall of the box 1. The temperature detector 14 is bidirectionally electrically connected to the controller 2. The temperature detector 14 monitors the temperature inside the box 1 in real time and transmits the detected data to the controller 2.

[0026] The enclosure 1 has a connecting groove 10 in the middle of the rear wall, a filter screen 11 inside the connecting groove 10, a cooler 12 fixedly connected to the front of the bottom wall of the connecting groove 10, and exhaust holes 13 on the front of the left and right ends of the enclosure 1. When controlled by the controller 2, the cooler 12 starts to run. External air enters the cooler 12 through the filter screen 11. The cooler 12 converts the air into cold air and injects it into the enclosure 1. The air inside the enclosure 1 is then discharged through the exhaust holes 13, thereby adjusting the temperature inside the enclosure 1.

[0027] The working principle of this utility model is as follows:

[0028] During the use of the high-throughput low-temperature centrifugation and dispensing equipment for plasma samples, the controller 2 controls the start of the air cooler 12. External air enters the air cooler 12 after being filtered by the filter screen 11. The air cooler 12 converts the air into cold air and injects it into the interior of the chamber 1. The air inside the chamber 1 is then discharged through the exhaust port 13, thereby adjusting the temperature inside the chamber 1. The temperature detector 14 monitors the temperature inside the chamber 1 in real time and transmits the detected data to the controller 2.

[0029] When the temperature reaches the set value (usually between 2°C and 8°C), the operator opens the door 4 and then passes the test tube containing the plasma sample through the mounting groove 3 and places it into the placement groove 8 in sequence. The length of the test tube containing the plasma sample is greater than the depth of the placement groove 8.

[0030] After placement, the operator rotates the adjustment knob 99. During rotation, the adjustment knob 99 drives the worm gear 98 to rotate via the mounting rod 97. During rotation, the worm gear 98 drives the worm wheel 96 to rotate via the meshing connection. During rotation, the worm wheel 96 drives the center wheel 95 to rotate via the upright rod 94. At this time, the fixed rod 93 slides inside the adjustment groove and rotates relative to the adjustment groove, thereby causing the center wheel 95 to drive the hexagonal sliding column 91 to move via the fixed rod 93. The hexagonal sliding column 91 drives the top plate 92 to move, and the top plate 92 drives the test tube containing the plasma sample to move, so that it contacts the inner wall of the placement groove 8, thereby realizing the installation and fixation of the test tube. Then the operator closes the box door 4.

[0031] Then, the servo motor 15 is started to run by the controller 2. The output shaft of the servo motor 15 will drive the support 6 to rotate. During the rotation of the support 6, the storage box 7 will drive the test tube containing the plasma sample to rotate. During the rotation of the test tube containing the plasma sample, centrifugal force is generated by high-speed rotation, thereby separating different components (such as blood cells, plasma, etc.) in the plasma sample.

[0032] After separation, the operator opens the door 4 and rotates the adjustment knob 99. During rotation, the adjustment knob 99 drives the worm gear 98 to rotate via the mounting rod 97. The worm gear 98, in turn, drives the worm wheel 96 to rotate via the meshing connection. The worm wheel 96, in turn, drives the central wheel 95 to rotate via the upright rod 94. At this time, the fixed rod 93 slides inside the adjustment groove and rotates relative to the adjustment groove, thereby causing the central wheel 95 to move the hexagonal sliding column 91 via the fixed rod 93. The hexagonal sliding column 91 then moves the top plate 92, finally separating the top plate 92 from the test tube containing the plasma sample. The operator then removes the test tubes containing the plasma sample one by one, and the separated blood samples can then be further aliquoted.

[0033] It is worth noting that the controller 2 disclosed in the above embodiments can be an STM8S207S8T6C, the air cooler 12 can be a MY-JYJY-L5-57, the temperature sensor 14 can be an OHR-E700, and the servo motor 15 can be an ECMA-C20604RS. The controller 2 controls the operation of the air cooler 12, the temperature sensor 14, and the servo motor 15 using methods commonly used in the prior art.

[0034] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A high-throughput low-temperature centrifugation and dispensing device for plasma samples, comprising a housing (1), a mounting groove (3) provided in the middle of the top wall of the housing (1), a door (4) hinged to the front of the upper end of the housing (1), the door (4) corresponding to the upper and lower positions of the mounting groove (3), a storage box (7) provided on the upper side inside the housing (1), a placement groove (8) provided on the upper end of the storage box (7), and a cold air blower (12) provided on the rear side inside the housing (1), characterized in that: It also includes fixed mechanisms (9); The fixing mechanism (9) includes a fixing rod (93), a vertical rod (94), and a central wheel (95). The vertical rod (94) is rotatably connected to the middle of the bottom wall of the storage box (7). A central wheel (95) is provided on the upper side of the outer arc surface of the vertical rod (94). Fixing rods (93) are respectively provided on the upper side inside the storage box (7). The lower ends of the five fixing rods (93) are respectively located in the adjustment grooves opened at the upper ends of the vertically adjacent central wheels (95).

2. The high-throughput low-temperature centrifugation and dispensing device for plasma samples according to claim 1, characterized in that: It also includes a controller (2), which is located at the top of the housing (1). The input end of the controller (2) is electrically connected to an external power source, and the input end of the air cooler (12) is electrically connected to the output end of the controller (2).

3. A high-throughput low-temperature centrifugation and dispensing device for plasma samples according to claim 1, characterized in that: The fixing mechanism (9) also includes a hexagonal sliding column (91) and a top plate (92). The hexagonal sliding column (91) is slidably connected to the upper side of the inner arc surface of the placement groove (8). The end of the hexagonal sliding column (91) away from the center of the storage box (7) is provided with a top plate (92). The lower end of the hexagonal sliding column (91) near the center of the storage box (7) is fixedly connected with a fixing rod (93).

4. A high-throughput low-temperature centrifugation and dispensing device for plasma samples according to claim 1, characterized in that: The fixing mechanism (9) also includes a worm gear (96), a mounting rod (97), a worm (98), and an adjustment knob (99). The worm gear (96) is located on the lower side of the outer arc surface of the upright (94). The mounting rod (97) is rotatably connected to the middle of the front wall of the storage box (7). The worm (98) is located on the rear side of the outer arc surface of the mounting rod (97). The worm gear (96) and the worm (98) are meshed and connected. The front end of the mounting rod (97) is provided with an adjustment knob (99).

5. A high-throughput low-temperature centrifugation and dispensing device for plasma samples according to claim 2, characterized in that: A fixing plate (5) is provided on the lower side inside the box (1). A support base (6) is rotatably connected to the middle of the upper end of the fixing plate (5). A storage box (7) is provided on the upper end of the support base (6). A servo motor (15) is provided in the middle of the bottom wall of the box (1). The upper end of the output shaft of the servo motor (15) is fixedly connected to the lower end of the support base (6). The input end of the servo motor (15) is electrically connected to the output end of the controller (2).

6. A high-throughput low-temperature centrifugation and dispensing device for plasma samples according to claim 2, characterized in that: A temperature detector (14) is installed on the front side of the bottom wall of the box (1), and the temperature detector (14) is bidirectionally electrically connected to the controller (2).

7. A high-throughput low-temperature centrifugation and dispensing device for plasma samples according to claim 1, characterized in that: A connecting groove (10) is provided in the middle of the rear wall of the box (1). A filter screen (11) is provided on the rear side inside the connecting groove (10). A cold air blower (12) is fixedly connected to the front side of the bottom wall of the connecting groove (10). Exhaust holes (13) are provided on the front sides of the left and right ends of the box (1).

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