High performance liquid chromatography tandem mass spectrometry detection of liquid storage tanks

Abstract

The application relates to the technical field of high-performance liquid chromatography, in particular to a high-performance liquid chromatography tandem mass spectrometry liquid phase storage tank, which comprises a storage tank, the lower part of the outer surface of the storage tank is fixedly connected with a discharge pipe, the upper end of the discharge pipe is fixedly connected with a supporting plate in the middle, the upper end of the supporting plate is fixedly connected with a power box, the right part of the upper end of the storage tank is fixedly connected with a feeding pipe, the inner pipe wall of the feeding pipe is provided with a clamping groove, and the middle part of the upper end of the storage tank is fixedly connected with a driving motor. The application is characterized in that the double-layer filter frame is adaptively clamped with the clamping groove of the feeding pipe, and the stirring assembly is linked and designed, so that high-efficiency two-stage filtration of the liquid phase entering the storage tank and sufficient stirring of the liquid phase in the tank are realized, impurities are effectively prevented from blocking the chromatographic column, the liquid phase is prevented from being precipitated and stratified, the detection cost is reduced, and the detection result accuracy is improved.

Description

Technical Field

[0001] This application relates to the field of high performance liquid chromatography technology, and in particular to high performance liquid chromatography-tandem mass spectrometry detection liquid storage tanks. Background Technology

[0002] In the high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection process, the liquid phase storage tank is a key component to ensure the smooth conduct of the detection. Its performance directly affects the accuracy and reliability of the detection results. In this technical field, the liquid phase needs to be kept pure and homogeneous during storage to prevent impurities from mixing in and components from separating, so as to ensure that the liquid phase entering the chromatograph meets the detection requirements.

[0003] A search revealed that Chinese Patent Publication No. CN219417348U discloses a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection liquid storage tank, comprising a tank body, an open top surface of the tank body with a cover, a liquid outlet on the bottom surface of the tank body, a liquid inlet on the top surface of the cover, and a filter device inside the tank body. The filter device includes a support assembly, which comprises an upper support frame, a lower support frame, and a pull rod. The pull rod is positioned above the middle of the top surface of the upper support frame and extends upward. The upper support frame and the lower support frame are fixedly connected.

[0004] Regarding the aforementioned technologies, the inventors discovered the following drawbacks: Although a filtration design is present, the filtration structure is simplistic, relying solely on a simple filter screen, which is insufficient to effectively intercept minute particulate impurities. This results in the liquid phase entering the chromatograph being prone to impurities, easily causing column blockage. Since chromatographic columns are expensive, blockage significantly increases detection costs. Furthermore, the installation method of the filter components within the storage tank is not reasonable, lacking a convenient disassembly structure. When a large amount of impurities accumulates on the filter screen, operators find it difficult to quickly remove and clean it, severely affecting the subsequent filtration effect of the liquid phase and greatly reducing work efficiency. This fails to meet the requirements for efficient and accurate detection, yet this patented technology does not provide a solution to this problem. Utility Model Content

[0005] To address the problems mentioned in the background section, this application provides a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection liquid storage tank.

[0006] The high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection liquid storage tank provided in this application adopts the following technical solution: it includes a storage tank, a discharge pipe is fixedly connected to the lower part of the outer surface of the storage tank, a support plate is fixedly connected to the middle of the upper end of the discharge pipe, a power supply box is fixedly connected to the upper end of the support plate, a feed pipe is fixedly connected to the upper right part of the storage tank, a groove is opened in the inner wall of the feed pipe, and a drive motor is fixedly connected to the middle of the upper end of the storage tank.

[0007] Optionally, the output end of the drive motor passes through the storage tank and is fixedly connected to a rotating rod. A connecting shaft block is fixedly connected to the upper part of the outer surface of the rotating rod. Six stirring rods are fixedly connected to the outer surface of the connecting shaft block. A connecting mesh frame is fixedly connected to the bottom of the stirring rods. Three L-shaped bottom rods are fixedly connected to the lower part of the connecting mesh frame. A scraper is fixedly connected to the bottom of each L-shaped bottom rod. The drive motor is fixedly connected to the middle of the upper end of the discharge pipe and is located directly below the support plate.

[0008] Optionally, a double-layer filter frame is snapped onto the upper part of the inner wall of the feed pipe. A coarse filter screen and a fine filter screen are fixedly connected to the upper and lower ends of the double-layer filter frame, respectively. Multiple backflow tilting plates are fixedly connected to the upper end of the double-layer filter frame. Handles are fixedly connected to the front and right sides of the upper end of the double-layer filter frame.

[0009] Optionally, the rotating rod vertically penetrates the top of the storage tank and is coaxially and fixedly connected to the output end of the drive motor, and the connecting shaft block is sleeved on the upper part of the outer surface of the rotating rod and fixedly connected.

[0010] Optionally, the six stirring rods are fixed at equal intervals along the circumferential direction of the outer surface of the connecting shaft block, and the connecting mesh frame is horizontally fixed to the bottom of the six stirring rods.

[0011] Optionally, the three L-shaped bottom bars are fixed at equal intervals along the circumferential direction of the lower end of the connecting frame, and the scraper is horizontally fixed to the bottom of the L-shaped bottom bars and fits against the bottom surface of the storage tank.

[0012] Optionally, the double-layer filter frame is rectangular, and the outer perimeter of the double-layer filter frame is fitted and engaged with the upper groove of the inner wall of the feed pipe.

[0013] Optionally, the coarse filter screen and the fine filter screen are inclined and fixed to the upper and lower ends of the double-layer filter frame, the inclination angle of the multiple backflow tilting plates is adapted to the inclination direction of the feed pipe and fixed to the upper end of the double-layer filter frame, and the two handles are symmetrically fixed to the front and right parts of the upper end of the double-layer filter frame.

[0014] In summary, this application includes the following beneficial technical effects:

[0015] This invention features a double-layer filter frame inside the feed pipe. A coarse filter fixed at the upper end and a fine filter fixed at the lower end form a two-stage filtration structure. The coarse filter first intercepts larger particulate impurities, while the fine filter further filters out smaller particulate impurities. Compared to the single-layer filtration structure of existing technologies, this significantly improves the filtration effect, effectively preventing impurities from entering the storage tank and contaminating the liquid phase, preventing column blockage due to impurities, and reducing detection costs. Furthermore, the outer perimeter of the double-layer filter frame is fitted with grooves on the upper part of the feed pipe wall and is equipped with handles for easy disassembly and cleaning, ensuring consistently stable and efficient filtration.

[0016] This invention features a drive motor located at the top center of a storage tank. Its output, via a rotating rod and connecting shaft, drives six equally spaced stirring rods to rotate, thus comprehensively agitating the liquid phase. A connecting mesh frame at the bottom of the stirring rods enhances structural strength. L-shaped bottom bars and a bottom scraper adhere to the bottom surface of the storage tank, ensuring thorough agitation of the bottom liquid phase and scraping away adhering materials. Compared to existing technologies, this invention more thoroughly prevents liquid phase precipitation and stratification, ensuring liquid phase homogeneity and providing high-quality samples with consistent properties for high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection, thereby improving the accuracy and reliability of detection results. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure in the embodiments of this application;

[0018] Figure 2 These are partial structural schematic diagrams of embodiments of this application;

[0019] Figure 3 This is a partial cross-sectional structural diagram of an embodiment of this application;

[0020] Figure 4 This is a partial split structure diagram of an embodiment of this application.

[0021] Reference numerals: 1. Discharge pipe; 2. Storage tank; 3. Feed pipe; 4. Slot; 5. Support plate; 6. Power supply box; 7. Drive motor; 8. Rotating rod; 9. Connecting shaft block; 10. Stirring rod; 11. Connecting mesh frame; 12. L-shaped bottom bar; 13. Scraper; 14. Double-layer filter frame; 15. Backflow inclined plate; 16. Coarse filter screen; 17. Fine filter screen; 18. Handle. Detailed Implementation

[0022] The following is in conjunction with the appendix Figure 1-4 This application will be described in further detail.

[0023] This application discloses a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection liquid storage tank. For example... Figure 1As shown, the system includes a storage tank 2. As the core component for storing the liquid phase, the storage tank 2 provides a stable and enclosed space for the liquid phase, effectively preventing contact between the liquid phase and the external environment, preventing contamination, ensuring its chemical stability, and meeting the purity and stability requirements of high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). A discharge pipe 1 is fixedly connected to the lower outer surface of the storage tank 2. The discharge pipe 1 is located at the lower outer surface of the storage tank 2 to facilitate the discharge of the liquid phase. During use, the liquid phase in the storage tank 2 can be smoothly discharged through the discharge pipe 1 for use in [specific applications]. Subsequent high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection and other operations ensure convenient liquid phase output. A support plate 5 is fixedly connected to the upper middle part of the discharge pipe 1. The support plate 5 is fixed to the upper middle part of the discharge pipe 1, providing a support base for the power supply box 6. The power supply box 6 is fixedly connected to the upper end of the support plate 5, which can provide a stable power supply to the electrical components in the entire storage tank device, such as the drive motor 7, ensuring that each component can operate normally and guaranteeing the continuity of operation of the HPLC-MS / MS detection liquid phase storage tank.

[0024] Please see Figure 1 , Figure 2 and Figure 3A feed pipe 3 is fixedly connected to the upper right side of the storage tank 2. The feed pipe 3 allows materials to smoothly enter the storage tank 2. A double-layer filter frame 14 is snapped onto the upper part of the inner wall of the feed pipe 3. The double-layer filter frame 14 is rectangular, and its outer perimeter is fitted and snapped into the groove 4 on the upper part of the inner wall of the feed pipe 3. A coarse filter screen 16 and a fine filter screen 17 are fixedly connected to the upper and lower ends of the double-layer filter frame 14, respectively. The rectangular double-layer filter frame 14 is attached to the inner wall of the feed pipe 3. The slot 4 of the part is adapted to ensure the stability of the double-layer filter frame 14 in the feed pipe 3. This shape and snap-fit ​​method can ensure the filtration effect, effectively intercept impurities, and ensure the quality of the liquid phase entering the storage tank 2. The coarse filter screen 16 and the fine filter screen 17 are fixed at the upper and lower ends of the double-layer filter frame 14 at an angle. The angled setting of the coarse filter screen 16 and the fine filter screen 17 is conducive to the impurities being filtered sliding down the surface of the filter screen, avoiding the accumulation of impurities. The filter screen is designed to reduce clogging, extend its service life, and lower maintenance costs while ensuring filtration efficiency and ensuring the continuous and smooth flow of liquid into storage tank 2. Multiple backflow tilting plates 15 are fixedly connected to the upper end of the double-layer filter frame 14. These plates change the flow direction of the material within the feed pipe 3, resulting in a more even distribution of material on the filter screen and improved filtration efficiency. The tilt angles of the multiple backflow tilting plates 15 are adapted to the tilt direction of the feed pipe 3 and fixed to the upper end of the double-layer filter frame 14. Handles 18 are fixedly connected to the front and right sides of the upper end of the double-layer filter frame 14. These two handles 18 are symmetrically fixed to the front and right sides of the upper end of the double-layer filter frame 14. This symmetrical arrangement of the handles 18 allows operators to grip the filter frame more easily and stably when disassembling and installing the double-layer filter frame 14, improving operational convenience and safety and reducing the risk of equipment damage or personal injury due to inconvenient operation. The inner wall of the feed pipe 3 has a groove 4.

[0025] Please see Figure 2A drive motor 7 is fixedly connected to the upper middle part of the storage tank 2. The drive motor 7, fixed at the upper middle part of the storage tank 2, provides a stable power source for the entire stirring device. Its position design ensures efficient power transmission, enabling stable operation of the stirring device. Stirring prevents precipitation and stratification of the liquid phase within the storage tank 2, ensuring the homogeneity of the liquid phase and meeting the sample homogeneity requirements of high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). The output end of the drive motor 7 passes through the storage tank 2 and is fixedly connected to a rotating rod 8. The rotating rod 8 vertically passes through the top of the storage tank 2 and is connected to the drive motor 7. The output end of motor 7 is coaxially fixedly connected. A connecting shaft block 9 is fixedly connected to the upper part of the outer surface of rotating rod 8. The connecting shaft block 9 is fixed to the upper part of the outer surface of rotating rod 8, providing a mounting base for stirring rod 10, so that stirring rod 10 can be firmly installed on rotating rod 8 and rotate with rotating rod 8 to perform stirring work, ensuring reliable connection between stirring component and power transmission component, and realizing stirring function. The connecting shaft block 9 is sleeved on the upper part of the outer surface of rotating rod 8 and fixedly connected. Six stirring rods 10 are fixedly connected around the outer surface of connecting shaft block 9. The six stirring rods 10 are arranged along the outer surface of connecting shaft block 9. Six stirring rods 10 are fixed at equal intervals around the outer surface of the connecting shaft block 9. This allows for comprehensive and uniform stirring of the material in the storage tank 2 when the rotating rod 8 rotates, improving stirring efficiency, ensuring thorough mixing, effectively preventing component separation in the liquid phase, and guaranteeing the consistency of the liquid phase properties. A connecting mesh frame 11 is fixedly connected to the bottom of all six stirring rods 10. Three L-shaped bottom rods 12 are fixedly connected to the lower perimeter of the connecting mesh frame 11. The three L-shaped bottom rods 12 extend along the connecting mesh frame 11. The L-shaped bottom rods 12 are fixed at equal intervals along their circumference. Each bottom of the L-shaped bottom rod 12 is fixedly connected to a scraper 13. The scraper 13 is horizontally fixed to the bottom of the L-shaped bottom rod 12 and fits against the bottom surface of the storage tank 2. The three L-shaped bottom rods 12 are fixed at equal intervals around the lower end of the connecting mesh frame 11. This allows the material at the bottom of the storage tank 2 to be stirred, preventing the material from accumulating at the bottom and ensuring that all the material in the storage tank 2 is fully stirred. This ensures that the liquid phase is uniform and avoids affecting the test results due to insufficient stirring of the bottom material. The drive motor 7 is fixedly connected to the middle of the upper end of the discharge pipe 1 and is located directly below the support plate 5.

[0026] The implementation principle of the high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection liquid phase storage tank in this application embodiment is as follows: When the HPLC-MS / MS detection liquid phase storage tank is working, the external liquid phase enters the storage tank 2 through the feed pipe 3. During this process, the coarse filter 16 and the fine filter 17 on the double-layer filter frame 14 perform two-stage filtration of the liquid phase. The coarse filter 16 first intercepts larger particulate impurities, and the fine filter 17 further filters smaller particulate impurities. The backflow inclined plate 15 makes the liquid phase evenly distributed on the filter screen to improve filtration efficiency. The filtered impurities slide down along the inclined filter screen to avoid clogging. The filtered liquid phase enters the storage tank 2, and the drive motor 7 is fixed to the storage tank. 2. At the upper middle part, after startup, the output end drives the rotating rod 8 to rotate. The connecting shaft block 9 on the rotating rod 8 drives the six equally spaced stirring rods 10 to rotate, stirring the liquid phase. The connecting mesh frame 11 enhances the structural strength of the stirring rods 10. The L-shaped bottom rod 12 and the bottom scraper 13 stir the liquid phase at the bottom of the storage tank 2 and scrape off the attached material to prevent sedimentation and adhesion. The support plate 5 and the power box 6 provide stable support and power supply for the drive motor 7 and other electrical components to ensure normal operation of the equipment. When the liquid phase is needed, the uniformly stirred and pure liquid phase can be discharged through the discharge pipe 1 at the lower part of the outer surface of the storage tank 2 for subsequent high performance liquid chromatography-tandem mass spectrometry detection.

[0027] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) detection liquid storage tank, comprising a storage tank (2), characterized in that: A discharge pipe (1) is fixedly connected to the lower part of the outer surface of the storage tank (2). A support plate (5) is fixedly connected to the middle of the upper end of the discharge pipe (1). A power supply box (6) is fixedly connected to the upper end of the support plate (5). A feed pipe (3) is fixedly connected to the right side of the upper end of the storage tank (2). A slot (4) is opened in the inner wall of the feed pipe (3). A drive motor (7) is fixedly connected to the middle of the upper end of the storage tank (2).

2. The high-performance liquid chromatography-tandem mass spectrometry detection liquid storage tank according to claim 1, characterized in that: The output end of the drive motor (7) passes through the storage tank (2) and is fixedly connected to a rotating rod (8). A connecting shaft block (9) is fixedly connected to the upper part of the outer surface of the rotating rod (8). Six stirring rods (10) are fixedly connected around the outer surface of the connecting shaft block (9). A connecting mesh frame (11) is fixedly connected to the bottom of the stirring rods (10). Three L-shaped bottom rods (12) are fixedly connected around the lower end of the connecting mesh frame (11). A scraper (13) is fixedly connected to the bottom of each L-shaped bottom rod (12). The drive motor (7) is fixedly connected to the middle of the upper end of the discharge pipe (1) and is located directly below the support plate (5).

3. The high-performance liquid chromatography-tandem mass spectrometry detection liquid storage tank according to claim 1, characterized in that: A double-layer filter frame (14) is snapped onto the upper part of the inner wall of the feed pipe (3). A coarse filter screen (16) and a fine filter screen (17) are fixedly connected to the upper and lower ends of the double-layer filter frame (14), respectively. Multiple backflow tilting plates (15) are fixedly connected to the upper end of the double-layer filter frame (14). A handle (18) is fixedly connected to the front and right sides of the upper end of the double-layer filter frame (14).

4. The high-performance liquid chromatography-tandem mass spectrometry detection liquid storage tank according to claim 2, characterized in that: The rotating rod (8) passes vertically through the top of the storage tank (2) and is coaxially and fixedly connected to the output end of the drive motor (7). The connecting shaft block (9) is sleeved on the upper part of the outer surface of the rotating rod (8) and fixedly connected.

5. The high-performance liquid chromatography-tandem mass spectrometry detection liquid storage tank according to claim 2, characterized in that: The six stirring rods (10) are fixed at equal intervals along the circumferential direction of the outer surface of the connecting shaft block (9), and the connecting mesh frame (11) is horizontally fixed to the bottom of the six stirring rods (10).

6. The high-performance liquid chromatography-tandem mass spectrometry detection liquid storage tank according to claim 2, characterized in that: The three L-shaped bottom rods (12) are fixed at equal intervals along the lower circumferential direction of the connecting frame (11), and the scraper (13) is horizontally fixed to the bottom of the L-shaped bottom rods (12) and fits against the inner bottom surface of the storage tank (2).

7. The high-performance liquid chromatography-tandem mass spectrometry detection liquid storage tank according to claim 3, characterized in that: The double-layer filter frame (14) is rectangular, and the outer perimeter of the double-layer filter frame (14) is fitted and snapped into the upper groove (4) of the inner wall of the feed pipe (3).

8. The high-performance liquid chromatography-tandem mass spectrometry detection liquid storage tank according to claim 3, characterized in that: The coarse filter screen (16) and fine filter screen (17) are fixed at the upper and lower ends of the double-layer filter frame (14) at an angle. The multiple backflow tilting plates (15) are fixed at the upper end of the double-layer filter frame (14) with their tilt angles matching the tilt direction of the feed pipe (3). The two handles (18) are symmetrically fixed at the front and right sides of the upper end of the double-layer filter frame (14).

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