A well plate for a DNA hydrogel biochip

Abstract

The utility model relates to the field of biological experimental apparatus, specifically relates to a well plate for DNA hydrogel biochip, including microwell plate and well plate cover, the microwell plate be the structure of the hole pipe that is uniformly distributed on its upper end face spacing, the position of the well plate cover lower end face on the corresponding hole pipe middle is provided with punch needle, the well plate cover cover is installed on microwell plate, the utility model design scientific and reasonable, has simple operation, saves the labour, punches the efficiency high, work reliable etc., is a kind of with higher innovative well plate for DNA hydrogel biochip.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment, and in particular to a well plate for DNA hydrogel biochips. Background Technology

[0002] In the field of single-cell cancer detection, tumor heterogeneity leads to insufficient accuracy of traditional detection methods. While single-cell technology can reveal cellular differences, it suffers from problems such as complex operation, high cost, and cell function damage. DNA hydrogel biochips based on nucleic acid molecular medicine offer a new direction for cancer biomarker detection. These biochips can specifically capture and sensitively detect cancer cells in complex clinical samples. By analyzing single-cell biomarkers, they reveal intercellular differences, accurately analyze tumor heterogeneity, and provide a new paradigm for tumor diagnosis.

[0003] Preparation of DNA hydrogel biochip: First, a gel block is prepared using agarose gel (polyacrylamide hydrogel). Then, holes are punched in the center of the gel block, and the required DNA strands are grown in the gel holes to form a DNA hydrogel.

[0004] This DNA hydrogel biochip is prepared on a 96-well plate. First, gel blocks are formed on the 96-well plate, and then holes are manually punched in the center of each block to create growth spaces for the DNA hydrogel. This method is cumbersome and labor-intensive. Furthermore, oversights can lead to missed holes, affecting the preparation of the DNA hydrogel biochip and subsequent experimental procedures. Summary of the Invention

[0005] The purpose of this invention is to provide a well plate for DNA hydrogel biochips to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: A microplate for DNA hydrogel biochips includes a microplate and a well plate cover. The microplate has a structure in which pores are evenly distributed on its upper end face. A perforation needle is provided on the lower end face of the well plate cover at the position corresponding to the center of the pores. The well plate cover is mounted on the microplate.

[0007] As a preferred embodiment of the present invention, the microporous plate includes a rectangular plate body, an outer frame is provided on the outer edge of the plate body, a support frame is provided on the outer wall below the outer frame, the upper end face of the support frame forms an overlapping platform for the microporous plate cover, a plurality of support plates are provided between the support frame and the bottom of the plate body, and an auxiliary mechanism is provided between the plate body and the microporous plate cover.

[0008] As a preferred embodiment of this utility model, the auxiliary mechanism includes screw holes, mounting bolts and auxiliary springs. Screw holes are provided at the four corners of the plate. An auxiliary spring is fixedly installed at the bottom of the screw hole. A mounting bolt threaded into the screw hole is pressed above the auxiliary spring. The mounting bolt is inserted into the screw hole through the hole plate cover.

[0009] As a preferred embodiment of this utility model, a positioning ring is provided on the lower end surface of the perforated plate cover corresponding to the position of the punching needle, the punching needle is located at the center of the positioning ring, the four walls of the perforated plate cover overlap the overlapping platform, and the perforated plate cover is provided with a lifting structure.

[0010] As a preferred embodiment of the present invention, the lifting structure includes a trapezoidal groove, a pair of trapezoidal grooves are formed on both sides of the perforated plate cover, a mounting bracket is provided in the trapezoidal groove, and a lifting member is hinged between the pair of mounting brackets.

[0011] Compared with the prior art, the above-mentioned technical solution of this utility model has the following beneficial technical effects: 1. This well plate for DNA hydrogel biochips is designed to allow simultaneous perforation of each gel block by manually punching holes in the gel blocks, compared to the existing method of manually punching holes in each block one by one. This greatly improves the perforation efficiency, effectively avoids missing holes, and ensures the reliability of subsequent experiments.

[0012] 2. This well plate for DNA hydrogel biochips features an auxiliary mechanism that allows the well plate cap to detach quickly from the microplate, facilitating efficient entry into the next DNA hydrogel growth stage and improving the fabrication efficiency of the DNA hydrogel biochip.

[0013] 3. This utility model has a scientific and reasonable design, and has the advantages of simple operation, saving manpower and effort, high drilling efficiency, and reliable operation. It is a highly innovative well plate for DNA hydrogel biochips. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall design of this utility model; Figure 2 This is a top view of the entire utility model; Figure 3 This is a diagram of the microporous plate of this utility model; Figure 4 This is a top view of the microporous plate of this utility model; Figure 5 This is a bottom view of the microporous plate of this utility model; Figure 6 This is a front view of the bottom of the microporous plate of this utility model; Figure 7 This is a bottom view of the perforated plate cover of this utility model.

[0015] Reference numerals: microplate 1, perforated tube 101, plate body 102, outer frame 103, support frame 104, support plate 105, auxiliary mechanism 3, screw hole 301, mounting bolt 302, auxiliary spring 303, perforated plate cover 2, drilling pin 201, positioning ring 202, placement frame 203, lifting structure 4, trapezoidal groove 401, mounting bracket 402, lifting component 403. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to specific embodiments and accompanying drawings. It should be understood that these descriptions are exemplary only and are not intended to limit the scope of this utility model. Furthermore, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessarily obscuring the concept of this utility model.

[0017] This utility model provides a technical solution: A well plate for DNA hydrogel biochips, such as Figures 1-6 As shown, it includes a microporous plate 1 and a perforated plate cover 2. The microporous plate 1 has a structure in which perforated tubes 101 are evenly distributed on its upper end surface. A perforating needle 201 is provided on the lower end surface of the perforated plate cover 2 at the position corresponding to the center of the perforated tubes 101. The perforating needle 201 is 11.9 mm long and 2.5 mm in diameter. The perforated plate cover 2 is installed on the microporous plate 1.

[0018] like Figures 2-4 As shown, the microporous plate 1 includes a rectangular plate body 102. Perforated tubes 101 are arranged in 12 columns along the length of the plate body 102 and in 8 rows along its width. The spacing between adjacent perforated tubes 101 is 9 mm. The top inner diameter of the perforated tube 101 is 6.8 mm, the bottom inner diameter is 6.21 mm, the depth of the perforated tube 101 is 11.7 mm, the thickness of the bottom of the perforated tube 101 is 1.2 mm, and the vertical distance from the top of the perforated tube 101 to the bottom of the support frame 104 is 14.3 mm. An outer frame 103 is provided on the outer edge of the plate body 102, and a support frame 104 is provided on the lower outer wall of the outer frame 103. The upper end face of the support frame 104 forms an overlapping platform for the perforated plate cover 2. Several support plates 105 are provided between the support frame 104 and the bottom of the plate body 102. An auxiliary mechanism 3 is provided between the plate body 102 and the perforated plate cover 2.

[0019] Because the punching needle 201 is inserted into the solid gel, there will be some resistance when opening the perforation plate cover 2. To facilitate the opening of the perforation plate cover 2, this utility model designs an auxiliary mechanism 3, such as... Figures 1-7As shown. The auxiliary mechanism 3 includes screw holes 301, mounting bolts 302, and auxiliary springs 303. Screw holes 301 are provided at the four corners of the plate 102. An auxiliary spring 303 is fixedly installed at the bottom of the screw holes 301. A mounting bolt 302, threaded into the screw hole 301, is pressed above the auxiliary spring 303. The mounting bolt 302 passes through the hole plate cover 2 into the screw hole 301. When opening the cover, the hole plate cover 2 can be lifted with the assistance of the auxiliary springs 303.

[0020] like Figure 2 , Figure 7 As shown, a positioning ring 202 is provided on the lower end surface of the perforated plate cover 2 at the position corresponding to the punch 201, the punch 201 is located at the center of the positioning ring 202, a mounting frame 203 is provided around the perforated plate cover 2, and a lifting structure 4 is provided on the perforated plate cover 2.

[0021] like Figure 2 As shown, the lifting structure 4 includes a trapezoidal groove 401. A pair of trapezoidal grooves 401 are opened on both sides of the perforated plate cover 2. A mounting bracket 402 is provided in the trapezoidal groove 401. A lifting member 403 is hinged between the pair of mounting brackets 402.

[0022] In practice, 3-aminopropyltriethoxysilane (APTES) is added to each well of the microplate 1 for surface amination modification.

[0023] Next, hydrogel preparation was performed. A 6% agarose gel or polyacrylamide hydrogel was prepared in microplate 1. Taking agarose gel as an example, 1.5 g of agarose was added to 25 mL of TBE buffer and melted in a microwave oven. After cooling to approximately 70°C, 100 μL of agarose solution was added to each well using the 6.8 mm inner diameter opening at the top of well tube 101. Then, well plate cap 2 was placed on top, and mounting bolt 302 was rotated into screw hole 301 to tighten the cap. In addition to its perforation function, well plate cap 2 also seals the solid gel to ensure the water content and stability of the agarose gel. After 30 minutes, the solution cooled and formed a solid gel.

[0024] Then, the mounting bolt 302 is slowly shaken and rotated. With the assistance of the auxiliary spring 303, the well plate cover is lifted. Then, the lifting member 403 is pulled up to open the well plate cover 2. The drilling needle 201 completes the drilling. The drilling area is the growth space for DNA hydrogel formation, thus initially obtaining a 96-micro-well array chip.

[0025] Finally, DNAs with different targeted functionalizations are grown in situ on the prepared chip to form a DNA hydrogel biochip. The DNA hydrogel can be reused. After the DNA hydrogel is dissolved, it can be re-prepared into a gel block. The DNA chains inside can be extracted using the gel electrophoresis method. These DNA chains can be used to prepare a new DNA hydrogel.

[0026] It should be understood that the above-described specific embodiments of this utility model are merely illustrative or explanatory of the principles of this utility model and do not constitute a limitation thereof. Therefore, any modifications, equivalent substitutions, improvements, etc., made without departing from the spirit and scope of this utility model should be included within the protection scope of this utility model. Furthermore, the appended claims are intended to cover all variations and modifications falling within the scope and boundaries of the appended claims, or equivalent forms of such scope and boundaries.

Claims

1. A well plate for DNA hydrogel biochips, characterized in that: It includes a microporous plate (1) and a perforated plate cover (2). The microporous plate (1) has a structure with evenly spaced perforated tubes (101) on its upper end surface. A perforating needle (201) is provided on the lower end surface of the perforated plate cover (2) at the center of the perforated tubes (101). The perforated plate cover (2) is mounted on the microporous plate (1).

2. The well plate for DNA hydrogel biochips according to claim 1, characterized in that: The microporous plate (1) includes a rectangular plate body (102), an outer frame (103) is provided on the outer edge of the plate body (102), a support frame (104) is provided on the lower outer wall of the outer frame (103), the upper end face of the support frame (104) forms the overlapping platform of the perforated plate cover (2), a plurality of support plates (105) are provided between the support frame (104) and the bottom of the plate body (102), and an auxiliary mechanism (3) is provided between the plate body (102) and the perforated plate cover (2).

3. The well plate for DNA hydrogel biochips according to claim 2, characterized in that: The auxiliary mechanism (3) includes a screw hole (301), a mounting bolt (302) and an auxiliary spring (303). Screw holes (301) are provided at the four corners of the plate (102). An auxiliary spring (303) is fixedly installed at the bottom of the screw hole (301). A mounting bolt (302) with a threaded connection in the screw hole (301) is pressed above the auxiliary spring (303). The mounting bolt (302) is inserted into the screw hole (301) by the hole plate cover (2).

4. A well plate for DNA hydrogel biochips according to claim 3, characterized in that: The lower end face of the perforated plate cover (2) is provided with a positioning ring (202) corresponding to the position of the punching needle (201). The punching needle (201) is located at the center of the positioning ring (202). The four walls (203) of the perforated plate cover overlap the overlapping platform. The perforated plate cover (2) is provided with a lifting structure (4).

5. A well plate for DNA hydrogel biochips according to claim 4, characterized in that: The lifting structure (4) includes a trapezoidal groove (401), a pair of trapezoidal grooves (401) are opened on both sides of the perforated plate cover (2), and a mounting bracket (402) is provided in the trapezoidal groove (401), and a lifting member (403) is hinged between the pair of mounting brackets (402).

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