A device for biomimetic mineralization

Abstract

The utility model discloses a kind of biological mineralization simulation devices, it is related to biological mineralization device technical field, specifically in solving the background art existing, in the biological mineralization process research with calcium carbonate as mineral main body, there is no suitable device that can simulate the biological mineralization process with calcium carbonate as mineral main body, a kind of biological mineralization simulation device is provided, including mineralization reaction tank, hot plate, pressure reducing valve and gas cylinder, wherein, hot plate is connected with the bottom of mineralization reaction tank, for heating to meet reaction temperature, pressure reducing valve is connected between mineralization reaction tank and gas cylinder, for controlling the gas pressure and flow rate of gas in mineralization reaction tank into gas cylinder. By using the biological mineralization simulation device of the application, it is closer to biological physiological environment, so that the biological mineralization process is closer to physiological state, and more representative mineralization results are obtained.

Description

Technical Field

[0001] This utility model belongs to the technical field of biomineralization devices, specifically relating to a biomineralization simulation device. Background Technology

[0002] Biomineralization refers to the precise regulation of the nucleation, crystallization, and growth of inorganic minerals by organisms through organic matter, resulting in biominerals with unique hierarchical structures and assembly methods, ultimately forming mineral structures with excellent mechanical properties (such as eggshells, nacre, and bones). Research on biomineralization not only provides a more intuitive understanding of its physiological processes but also offers important references for regulating mineralization processes under environmental conditions and synthesizing single-crystal or polycrystalline structures, promoting the widespread application of biomimetic pathways in inorganic solid synthesis.

[0003] Calcium carbonate is one of the most common biominerals, widely found in biological structures such as bird eggshells, mollusc shells, pearls, corals, and sea urchin spines, serving as their main inorganic mineral component. The biomineralization process of calcium carbonate typically involves the uptake of calcium and carbonate ions, which, under the regulation of organic matter, are deposited at mineralization sites to form mineral structures.

[0004] In existing technologies, various in vitro simulation methods have been developed based on the biomineralization mechanism of calcium carbonate. For example, calcium ion solution is used as the mineralization liquid in a closed environment, and carbon dioxide is released through the decomposition of ammonium carbonate to supply carbonate ions, or a solution containing carbonate ions is directly added to simulate the mineralization process of calcium carbonate. However, these methods generally lack controllability and have certain limitations.

[0005] In biomineralization, the mineralization rate is mainly affected by organic matter, calcium ion concentration, and the continuous supply of carbonate ions. The method of releasing carbon dioxide using ammonium carbonate has two major problems: first, the release rate is significantly affected by environmental factors such as temperature and humidity; second, the decomposition process of ammonium carbonate itself lacks controllability, making it difficult to precisely adjust the carbon dioxide release rate, resulting in unstable carbonate ion supply and an inability to control the rate. On the other hand, while directly adding a carbonate-containing solution is simple, the slow biomineralization process makes it difficult to maintain a stable reaction rate in long-term experiments. Furthermore, the activity of organic matter in biomineralization is highly dependent on ambient temperature, but existing experiments often lack effective temperature control, making it difficult to flexibly adjust reaction conditions according to experimental design, thus affecting the reproducibility and applicability of in vitro experimental results in real biological systems.

[0006] Therefore, based on the need for in vitro biomineralization simulation research of calcium carbonate and the problems existing in the above-mentioned simulation technology, it is necessary to design an in vitro biomineralization simulation device with calcium carbonate as the main mineral and controllable temperature and reaction rate to meet the needs of biomineralization-related research. Utility Model Content

[0007] To address the shortcomings of existing technologies, there is currently no suitable device to simulate the biomineralization process, particularly in studies involving calcium carbonate as the primary mineral, thus failing to meet the requirements for relevant research. This invention provides a biomineralization simulation device, comprising a mineralization reaction vessel, a heating plate, a pressure-reducing valve, and a gas cylinder. The heating plate is connected to the bottom of the mineralization reaction vessel to heat it to the required reaction temperature. The pressure-reducing valve is connected between the mineralization reaction vessel and the gas cylinder to control the gas pressure and flow rate of the gas flowing from the gas cylinder into the mineralization reaction vessel. By using this biomineralization simulation device, the reaction temperature and the flow rate of the gas involved in the reaction can be controlled, ultimately achieving a complete simulation of the mineralization environment within a living organism. This makes the mineralization process closer to the physiological state of the organism, resulting in more representative mineralization results.

[0008] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0009] A biomineralization simulation device includes a gas cylinder, characterized in that it further includes a mineralization reaction vessel, which has an open structure at both ends for carrying out a biomineralization reaction;

[0010] A heating plate is installed at the lower end of the mineralization reaction tank;

[0011] The pressure reducing valve is connected at one end to the mineralization reaction tank and at the other end to the gas cylinder.

[0012] Preferably, the mineralization reaction vessel includes a matching tank body and a cover, and the cover is provided with a connector and a multi-channel connector.

[0013] Preferably, the tank body has an open structure at both ends, and a gas dispersion plate and a support plate are respectively provided inside, with the gas dispersion plate located at the upper end of the support plate.

[0014] Preferably, the gas dispersion plate has several air leakage holes.

[0015] Preferably, the tray has several ventilation holes, and limit strips are slidably provided on two adjacent sides of the tray to limit the perforated plate.

[0016] Preferably, the end of the limiting strip is connected to a slider, the slider is slidably locked onto the support plate, and a fastening bolt is threaded onto the slider.

[0017] The beneficial effects of this utility model are as follows: This utility model discloses a biomineralization simulation device. Compared with the prior art, the improvement of this utility model lies in:

[0018] (1) By setting up a heating plate and a pressure reducing valve, this utility model can adjust the reaction environment inside the mineralization reaction tank, making the environment inside the mineralization reaction tank closer to the biological physiological environment, thereby making the mineralization process closer to the physiological state and obtaining more representative mineralization results.

[0019] (2) The tray provided by this utility model can disperse the hot air heated by the heating plate, so that the porous plate is heated more evenly. At the same time, it can limit the porous plates of different specifications to ensure their stability during the reaction process and avoid the reaction liquid from spilling out of the porous plate due to external force, causing the reaction to fail.

[0020] (3) The gas dispersion plate provided in this utility model can disperse the gas entering the mineralization reaction tank, so that it can react quickly and fully. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of the biomineralization simulation device of this utility model;

[0022] Figure 2 This is a front view of the mineralization reaction vessel of this utility model;

[0023] Figure 3 This is a perspective view of the tank body of this utility model;

[0024] Figure 4 This is a top view of the pallet of this utility model;

[0025] Figure 5 This is a diagram showing the limiting state of the pallet on the perforated plate according to this utility model.

[0026] The components include: 1. Mineralization reaction vessel; 101. Vessel body; 102. Cover; 1021. Connector; 1022. Multi-channel connector; 103. Limiting block; 2. Heating plate; 3. Pressure reducing valve; 4. Gas cylinder; 5. Gas dispersion plate; 501. Leakage hole; 6. Support plate; 601. Vent hole; 602. Slide groove one; 603. Slide groove two; 604. Limiting strip; 6041. Sliding block; 6042. Fastening bolt. Detailed Implementation

[0027] To enable those skilled in the art to better understand the technical solution of this utility model, the technical solution of this utility model will be further described below with reference to the accompanying drawings and embodiments. The following embodiments are used to illustrate this utility model, but should not be used to limit the scope of this utility model.

[0028] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" or "linked" should be interpreted broadly. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0029] Example:

[0030] See attached document Figure 1-3 The illustrated biomineralization simulation device includes a mineralization reaction tank 1, a heating plate 2, a pressure reducing valve 3, and a gas cylinder 4. The mineralization reaction tank 1 is used to simulate the biomineralization reaction of calcium carbonate, the heating plate 2 is used to control the reaction temperature inside the mineralization reaction tank 1, the pressure reducing valve 3 is used to control the gas pressure and flow rate of the gas entering the mineralization reaction tank 1, and the gas cylinder 4 is filled with carbon dioxide to supply gas to the mineralization reaction tank 1 to participate in the mineralization reaction.

[0031] In this embodiment, a pressure reducing valve 3 is connected to the mineralization reaction tank 1, and the other end of the pressure reducing valve 3 is connected to the gas cylinder 4 via a gas supply pipe. The pressure reducing valve 3 is a readily available and mature product, and this embodiment does not modify it.

[0032] The mineralization reaction vessel 1 has an open top and bottom structure. Its bottom is connected to the heating plate 2 via a sealing strip. After the heating plate 2 is turned on, it can heat the temperature inside the mineralization reaction vessel 1. The heating plate 2 adopts an existing mature product. In this embodiment, an adjustable temperature silicone heating plate with the brand name Dirui and a specification of 200*200MM can be selected. The heating plate 2 is connected to a power plug and a matching digital display temperature controller. After connecting the power plug to a fixed power supply or a mobile power supply, the heating plate 2 can be turned on to generate heat, thereby transmitting high temperature into the mineralization reaction vessel 1 to ensure the reaction temperature inside the mineralization reaction vessel 1. The heating plate 2 is also equipped with a digital display temperature controller, which is used to display the current heating temperature.

[0033] Furthermore, the mineralization reaction tank 1 includes a tank body 101 and a cover 102 that are adapted to each other. A plurality of connectors 1021 are provided on the cover 102, and the plurality of connectors 1021 are distributed around the cover 102. In this embodiment, there are four connectors 1021, which are distributed in the front, back, left and right positions of the cover 102, so that gas can enter the mineralization reaction tank 1 from different positions of the cover 102, thereby achieving uniform gas entry.

[0034] The cover 102 is also provided with a multi-channel connector 1022, which can be connected to several connectors 1021 through pipelines, and is also connected to the pressure reducing valve 3. When carbon dioxide in the gas cylinder 4 enters the pressure reducing valve 3, the pressure reducing valve 3 can be used to control the gas pressure and flow rate (i.e., release rate) of carbon dioxide entering the connector 1021, so as to meet the requirements of carbon dioxide gas and flow rate in the mineralization reaction tank 1.

[0035] The tank body 101 is provided with a gas dispersion plate 5 and a support plate 6. The gas dispersion plate 5 is detachably installed inside the tank body 101. Specifically, limit blocks 103 are fixedly installed at the four corners of the inner wall of the tank body 101, and the gas dispersion plate 5 rests on the limit blocks 103.

[0036] The gas dispersion plate 5 is provided with a plurality of vent holes 501 for dispersing carbon dioxide entering the mineralization reaction tank 1, so that the gas passing through the gas dispersion plate 5 is further dispersed, which is beneficial to the reaction. In this embodiment, there are ninety-six vent holes 501.

[0037] See attached document Figure 4-5 As shown, the tray 6 is fixedly installed inside the tank 101 and has a plate-like structure. It is provided with a number of vent holes 601. In this embodiment, there can be ninety-six vent holes 601 to facilitate the dispersion of the hot air heated by the heating plate 2 and to make it evenly act on the porous plate used in the experiment.

[0038] In use, the perforated plate is placed on the tray 6. To ensure the stability of the perforated plate on the tray 6, a first groove 602 and a second groove 603 are provided on two adjacent sides of the tray 6. A limit strip 604 is slidably provided in the first groove 602 and the second groove 603 to limit the perforated plate placed on the tray 6, so as to prevent it from moving due to external force and causing the reaction inside to spill out easily.

[0039] Specifically, a slider 6041 is fixedly connected to the bottom of the limiting strip 604. The slider 6041 is slidably engaged in the first slide groove 602 and the second slide groove 603. A fastening bolt 6042 is provided on the slider 6041. The fastening bolt 6042 is threadedly connected to the slider 6041 to position the slider 6041 in the first slide groove 602 and the second slide groove 603, thereby realizing the positioning of the limiting strip 604.

[0040] The biomineralization simulation device of this preferred embodiment simulates the mineralization process of all biominerals with calcium carbonate as the main mineral component as follows:

[0041] First, heating plate 2 is turned on according to experimental requirements and heated to the set temperature to ensure that the mineralization reaction vessel reaches the required reaction temperature before the mineralization experiment is carried out. Then, a suitable multi-well plate (such as a 96-well, 48-well, 24-well, 12-well, 8-well, 6-well, or 4-well plate) is selected, and a calcium ion solution (preferably calcium chloride solution) and a mineralization substrate (such as a silicon wafer, natural substrate material, or biomimetic membrane) are added to each well of the multi-well plate. According to the experimental design, a certain concentration of organic matter (such as proteins, peptides, etc.) can be further added to the wells to simulate the regulatory role of organic templates in biomineralization.

[0042] Next, the prepared porous plate is placed on the support plate 6, and the sliders 6041 in the first slide groove 602 and the second slide groove 603 are slid respectively, so that the limiting strip 604 can limit the two adjacent sides of the porous plate, thereby fixing the porous plate on the support plate 6.

[0043] Subsequently, pressure reducing valve 3 was opened, and the appropriate carbon dioxide flow rate was adjusted to control the gas concentration entering the reaction vessel in order to regulate the mineralization rate. The reaction continued for a set time. After the experiment was completed, the porous plate was removed and the calcium carbonate minerals formed in the pores were collected for subsequent analysis and characterization.

[0044] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A biomineralization simulation device, comprising a gas cylinder (4), characterized in that, Also includes: The mineralization reaction vessel (1) has an open structure at both ends and is used for biomineralization reaction; A heating plate (2) is installed at the lower end of the mineralization reaction tank (1); The pressure reducing valve (3) is connected at one end to the mineralization reaction tank (1) and at the other end to the gas cylinder (4).

2. The biomineralization simulation device according to claim 1, characterized in that, The mineralization reaction vessel (1) includes a matching vessel body (101) and a cover (102), and a connector (1021) and a multi-channel connector (1022) are provided on the cover (102).

3. The biomineralization simulation device according to claim 2, characterized in that, The tank (101) has an open structure at both ends, and a gas dispersion plate (5) and a support plate (6) are respectively installed inside it, with the gas dispersion plate (5) located at the upper end of the support plate (6).

4. The biomineralization simulation device according to claim 3, characterized in that, The gas dispersion plate (5) has several air leakage holes (501).

5. The biomineralization simulation device according to claim 3, characterized in that, The tray (6) has several ventilation holes (601) and limit strips (604) are slidably provided on two adjacent sides of the tray (6) to limit the perforated plate.

6. The biomineralization simulation device according to claim 5, characterized in that, The end of the limiting strip (604) is connected to a slider (6041), which slides and is locked on the support plate (6), and a fastening bolt (6042) is threaded onto the slider (6041).

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