A leaf PCR rapid sampling device

Abstract

The utility model relates to the technical field of blade sampling, disclose a kind of blade PCR rapid sampling device, including mounting block;Connecting block is fixedly connected in the outside right side of the mounting block;Adapter block and placement plate, the adapter block is inserted in the outside right side of the mounting block, the placement plate is fixedly connected in the outside of the adapter block, the hole is opened in the middle of the placement plate, and the hole is used to place sampling tube;Fixed link and two moving blocks, the fixed link is fixedly connected in the inside of the adapter block, and two the moving block is slidably connected in the outside of the fixed link.The utility model in the present application, when the sampling head needs to be cleaned, the placement plate is conveniently removed from the lower side of the sampling head, so that the cleaning of the sampling head is more convenient and thorough, ensures that there is no residual material, reduces the risk of cross-contamination, and ensures the purity of subsequent samples and the accuracy of experimental results.

Description

Technical Field

[0001] This utility model relates to the field of leaf sampling technology, and in particular to a leaf PCR rapid sampling device. Background Technology

[0002] Leaf PCR is a technique used to extract DNA from plant leaves and amplify it for analysis. This technique is widely used in plant gene research, breeding, and disease detection. Leaf PCR rapid sampling devices are tools used to quickly and efficiently extract samples from plant leaves for PCR analysis. Their main purpose is to improve sampling efficiency, reduce sample processing time, and ensure the integrity and purity of the samples.

[0003] When using the existing leaf PCR rapid sampling device, first, place the sampling tube into the tube slot, take a leaf and place it in the middle of the sampler, manually press the sampler, and the sampler will make a hole in the leaf. The leaf sampled in the hole falls into the sampling tube, and the leaf sampling operation is completed.

[0004] Although existing leaf PCR rapid sampling devices can complete the leaf sampling operation, it is not convenient to clean the sampling head. Residual plant tissue or DNA on the sampling head can contaminate subsequent samples, leading to inaccurate experimental results and affecting the quality of subsequent samples. Utility Model Content

[0005] To overcome the above shortcomings, this invention provides a leaf PCR rapid sampling device, which aims to improve the problem in the prior art that it is inconvenient to clean the sampling head, and that residual plant tissue or DNA on the sampling head will contaminate subsequent samples, leading to inaccurate experimental results.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A leaf PCR rapid sampling device, comprising:

[0008] Mounting block;

[0009] A connecting block, which is fixedly connected to the outer right side of the mounting block;

[0010] A connecting block and a placement plate are provided. The connecting block is inserted into the outside right side of the mounting block, and the placement plate is fixedly connected to the outside of the connecting block. A hole is provided in the middle of the placement plate for placing a sampling tube.

[0011] A fixed rod and two movable blocks are provided, wherein the fixed rod is fixedly connected inside the connecting block, and the two movable blocks are slidably connected outside the fixed rod.

[0012] Two pressing rods and two L-shaped inserts, the two pressing rods being fixedly connected to the top of the two moving blocks, and the two L-shaped inserts being fixedly connected to the bottom of the two moving blocks;

[0013] Spring 2, which is sleeved on the outside of the fixed rod;

[0014] And a limiting component, which is used to fix the sampling tube.

[0015] Furthermore, the limiting component includes an arc-shaped clamping plate one, which is fixedly connected to the bottom left side of the placement plate. A horizontal plate is fixedly connected to the bottom right side of the placement plate. A lead screw is threaded inside the horizontal plate. An arc-shaped clamping plate two is rotatably connected to the outer left side of the lead screw. A handwheel is fixedly connected to the outer right side of the lead screw.

[0016] Furthermore, a protrusion is fixedly connected to the upper left side of the mounting block, and a handle is rotatably connected to the outside of the protrusion. A crossbar is provided inside the connecting block, and a fixing block is fixedly connected to the top of the crossbar. The top of the fixing block contacts the handle. A sampling head is fixedly connected to the bottom of the crossbar, and a spring is fixedly connected to the top of the connecting block. The spring is sleeved on the outside of the crossbar.

[0017] Furthermore, the lower two sides of the connecting block are provided with through holes one, and the two L-shaped inserts are slidably connected inside the two through holes one. The upper two sides of the connecting block are provided with through holes two, and the two pressing rods are slidably connected inside the two through holes two.

[0018] Furthermore, a sliding hole is provided inside the connecting block, and the crossbar and the sampling head are slidably connected inside the sliding hole.

[0019] Furthermore, an installation groove is provided on the outer right side of the mounting block, and the connecting block is inserted into the installation groove.

[0020] Furthermore, each of the mounting blocks has a slot on its inner right side, and the two L-shaped rods are inserted into the two slots. A lifting lug is fixedly connected to the outer left side of the mounting block.

[0021] Furthermore, both the first and second arc-shaped clamps are provided with protective pads inside, and both protective pads are made of rubber.

[0022] This utility model has the following beneficial effects:

[0023] 1. In this utility model, when cleaning the sampling head, first press the pressing rod relative to each other, which drives the moving block and the L-shaped insert to move synchronously, while compressing the second spring. When the L-shaped insert disengages from the slot, pull out the placement plate and connecting block to clean the sampling head. After cleaning, align the connecting block with the installation slot and insert it. Release the pressing rod, and the second spring will reset so that the L-shaped insert is inserted into the slot, completing the installation. This design facilitates thorough cleaning of the sampling head, avoids residue and cross-contamination, and ensures sample purity and accuracy of experimental results.

[0024] 2. In this utility model, when fixing the sampling tube, it is inserted into the hole of the placement plate so that the bottom of the tube is in contact with the first arc-shaped clamp. The handwheel is turned to drive the screw to rotate, pushing the second arc-shaped clamp to clamp the sampling tube. Through the cooperation of the first and second arc-shaped clamps, stable fixing is achieved, preventing shaking or tipping during sampling, ensuring that the sample falls accurately into the tube, and improving sampling stability. Attached Figure Description

[0025] Figure 1 This is a perspective view of a leaf PCR rapid sampling device proposed in this utility model;

[0026] Figure 2 This is a schematic diagram of the disassembled structure of the placement plate of the leaf PCR rapid sampling device proposed in this utility model;

[0027] Figure 3 This is a schematic diagram of the internal structure of the mounting block of a leaf PCR rapid sampling device proposed in this utility model;

[0028] Figure 4 This is a schematic diagram of the lifting lug structure of a leaf PCR rapid sampling device proposed in this utility model;

[0029] Figure 5 This is a schematic diagram of the sampling head structure of a leaf PCR rapid sampling device proposed in this utility model;

[0030] Figure 6 for Figure 3 Enlarged view of the structure at point A in the middle;

[0031] Figure 7 for Figure 4 Enlarged view of the structure at point B.

[0032] Legend:

[0033] 1. Mounting block; 2. Lifting lug; 3. Connecting block; 4. Protrusion; 5. Handle; 6. Fixing block; 7. Crossbar; 8. Spring 1; 9. Placement plate; 10. Connecting block; 11. Fixing rod; 12. Moving block; 13. Pressing rod; 14. Spring 2; 15. L-shaped insert rod; 16. Through hole 1; 17. Slot; 18. Through hole 2; 19. Arc-shaped clamp 1; 20. Protective pad; 21. Crossbar; 22. Screw rod; 23. Arc-shaped clamp 2; 24. Handwheel; 25. Sampling head; 26. Sliding hole; 27. Mounting groove. Detailed Implementation

[0034] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0035] Reference Figure 1 , Figure 3 Figure 6 This utility model provides an embodiment of a leaf PCR rapid sampling device, comprising: a mounting block 1; a connecting block 3, which is fixedly connected to the outer right side of the mounting block 1; a connecting block 10 and a placement plate 9, wherein the connecting block 10 is inserted into the outer right side of the mounting block 1, and the placement plate 9 is fixedly connected to the outside of the connecting block 10, and the placement plate 9 has a hole in the middle for placing a sampling tube; a fixing rod 11 and two moving blocks 12, wherein the fixing rod 11 is fixedly connected to the inside of the connecting block 10, and the two moving blocks 12 are slidably connected to the outside of the fixing rod 11; two pressing rods 13 and two L-shaped insert rods 15, wherein the two pressing rods 13 are fixedly connected to the top of the two moving blocks 12, and the two L-shaped insert rods 15 are fixedly connected to the bottom of the two moving blocks 12; and a second spring 14. 4 are set on the outside of the fixed rod 11; and a limiting component is used to fix the sampling tube. The connecting block 10 has through holes 16 on both sides of the lower part. Two L-shaped insert rods 15 are slidably connected inside the two through holes 16. The through holes 16 facilitate the movement of the L-shaped insert rods 15. The connecting block 10 has through holes 28 on both sides of the upper part. Two pressing rods 13 are slidably connected inside the two through holes 28. The through holes 28 facilitate the first pressing of the pressing rods 13. The mounting block 1 has a mounting groove 27 on the right side of the outside. The connecting block 10 is inserted into the mounting groove 27. The mounting block 1 has a slot 17 on the right side of the inside. The two L-shaped insert rods 15 are inserted into the two slots 17. The mounting block 1 has a lifting lug 2 fixedly connected to the left side of the outside. The lifting lug 2 facilitates carrying the sampler.

[0036] Specifically, when cleaning the sampling head 25 is required, the operator first needs to press the two pressing rods 13 relative to each other. At this time, the two pressing rods 13 will drive the two fixed moving blocks 12 at the bottom to move relative to each other outside the fixed rod 11. During the relative movement of the two moving blocks 12, they will drive the two fixed L-shaped inserts 15 at the bottom to move synchronously. At the same time, the relative movement of the two moving blocks 12 will compress the spring 14 sleeved in the middle of the fixed rod 11, causing the spring 14 to deform under force. Subsequently, when the two L-shaped inserts 15 disengage from the inside of the two slots 17, the operator can move the placement plate 9 outward. The movement of the placement plate 9 will drive the externally fixed connecting block 10 to move, causing the connecting block 10 to disengage from the inside of the mounting block 1. At this point, the operator can directly access the sampling head 25 for cleaning. After cleaning, the operator needs to align the connecting block 10 fixed to the outside of the placement plate 9 with the mounting groove 27 opened on the outside of the mounting block 1. Then, the connecting block 10 is inserted into the inside of the mounting groove 27. Finally, the two pressing rods 13 are released, and under the reaction force of the spring 14, the two L-shaped inserts 15 are inserted into the inside of the two slots 17, thus completing the installation. This makes it easy to remove the placement plate 9 from below the sampling head 25 when cleaning is required, making the cleaning of the sampling head 25 more convenient and thorough. In this way, it is ensured that there are no residual substances, reducing the risk of cross-contamination, thereby ensuring the purity of subsequent samples and the accuracy of experimental results.

[0037] Reference Figure 2 , Figure 4 and Figure 5 The limiting component includes an arc-shaped clamping plate 19, which is fixedly connected to the bottom left side of the placement plate 9. A horizontal plate 21 is fixedly connected to the bottom right side of the placement plate 9. A lead screw 22 is threadedly connected inside the horizontal plate 21. An arc-shaped clamping plate 23 is rotatably connected to the outer left side of the lead screw 22. A handwheel 24 is fixedly connected to the outer right side of the lead screw 22. The handwheel 24 facilitates the use of the operator. Both the arc-shaped clamping plate 19 and the arc-shaped clamping plate 23 are equipped with protective pads 20. Both protective pads 20 are made of rubber. Rubber has good friction and can effectively prevent the object from sliding during clamping, ensuring the stability of the sampling tube or other clamped objects.

[0038] Specifically, when fixing the sampling tube, firstly, the sampling tube is inserted into the hole in the middle of the placement plate 9, so that the bottom of the sampling tube is in contact with the outside of the arc-shaped clamp 19. Then, the operator turns the handwheel 24, which drives the externally fixed screw 22 to rotate inside the horizontal plate 21. As the screw 22 rotates, the arc-shaped clamp 23 connected to the left side also moves until the arc-shaped clamp 23 contacts the outside of the sampling tube. In this way, the arc-shaped clamp 19 and the arc-shaped clamp 23 wrap around the sampling tube, thereby completing the fixing operation of the sampling tube. This makes it easy to fix the sampling tube inserted inside the placement plate 9 during sampling. The fixed sampling tube will not shake or tip over during the sampling process, ensuring that the sample can fall accurately into the tube, thereby improving the stability of the sampling.

[0039] Reference Figure 1 , Figure 2 and Figure 3 A protrusion 4 is fixedly connected to the upper left side of the mounting block 1. A handle 5 is rotatably connected to the outside of the protrusion 4. A crossbar 7 is set inside the connecting block 3. A fixing block 6 is fixedly connected to the top of the crossbar 7. The top of the fixing block 6 contacts the handle 5. A sampling head 25 is fixedly connected to the bottom of the crossbar 7. A spring 8 is fixedly connected to the top of the connecting block 3. The spring 8 is sleeved on the outside of the crossbar 7. A sliding hole 26 is opened inside the connecting block 3. The crossbar 7 and the sampling head 25 are slidably connected inside the sliding hole 26. The sliding hole 26 facilitates the movement of the crossbar 7 and the sampling head 25.

[0040] Specifically, during sampling, firstly, the sampling tube is placed in the hole in the middle of the placement plate 9 and fixed. Next, the blade is placed on the upper part of the placement plate 9. Then, the handle 5 is pressed, and the handle 5 applies a downward force to the fixing block 6, causing the crossbar 7 fixed at the bottom of the fixing block 6 to move downward inside the connecting block 3. When the crossbar 7 moves, it compresses the spring 8 sleeved on the outside, causing the spring 8 to deform under force. Subsequently, when the crossbar 7 moves downward, it moves the sampling head 25 fixed at the bottom downward in sync. The sampling head 25 moves downward and contacts the blade, making a hole in the blade. The blade sampled in the hole falls into the sampling tube, thus completing the blade sampling operation. After sampling is completed, the handle 5 is released, and the sampling head 25 and the crossbar 7 are reset under the reaction force of the spring 8.

[0041] Working principle: When cleaning the sampling head 25 is required, firstly, the two pressing rods 13 are squeezed relative to each other. The two pressing rods 13, along with the two movable blocks 12 fixed at the bottom, move relative to each other outside the fixed rod 11. As the two movable blocks 12 move relative to each other, they also move synchronously with the two L-shaped inserts 15 fixed at the bottom. Simultaneously, the two movable blocks 12 compress the spring 14 sleeved in the middle of the fixed rod 11, causing the spring 14 to deform under force. Then, when the two L-shaped inserts 15 disengage from the two slots 17, the placement plate 9 can be moved outwards. The movement of the placement plate 9 moves the externally fixed connecting block 10, causing the connecting block 10 to disengage from the mounting block 1. At this point... Operators can directly access the sampling head 25 for cleaning. After cleaning, align the connecting block 10 fixed to the outside of the placement plate 9 with the mounting groove 27 opened on the outside of the mounting block 1. Then, insert the connecting block 10 into the inside of the mounting groove 27. Finally, release the two pressing rods 13. Under the reaction force of the spring 14, insert the two L-shaped inserts 15 into the inside of the two slots 17. The installation is then complete. This makes it easy to remove the placement plate 9 from below the sampling head 25 when cleaning is required, making the cleaning of the sampling head 25 more convenient and thorough, ensuring no residue, reducing the risk of cross-contamination, and ensuring the purity of subsequent samples and the accuracy of experimental results.

[0042] When fixing the sampling tube, firstly, insert the sampling tube into the hole in the middle of the placement plate 9, so that the bottom of the sampling tube is in contact with the outside of the arc-shaped clamp 19. Then, turn the handwheel 24 to rotate the externally fixed screw 22 inside the horizontal plate 21. The screw 22 moves the externally connected arc-shaped clamp 23 on the left side, so that the arc-shaped clamp 23 contacts the outside of the sampling tube. Thus, the sampling tube is wrapped by the arc-shaped clamp 19 and the arc-shaped clamp 23, and the fixing operation of the sampling tube is completed. This makes it easy to fix the sampling tube inserted inside the placement plate 9 during sampling. The fixed sampling tube will not shake or tip over during the sampling process, ensuring that the sample can fall accurately into the tube, thereby improving the stability of sampling.

[0043] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A leaf PCR rapid sampling device, characterized in that, include: Install block (1); Connecting block (3), which is fixedly connected to the outside right side of the mounting block (1); A connecting block (10) and a placement plate (9) are provided. The connecting block (10) is inserted into the outside right side of the mounting block (1). The placement plate (9) is fixedly connected to the outside of the connecting block (10). A hole is provided in the middle of the placement plate (9) for placing a sampling tube. A fixed rod (11) and two movable blocks (12) are provided, wherein the fixed rod (11) is fixedly connected inside the connecting block (10), and the two movable blocks (12) are slidably connected outside the fixed rod (11); Two pressing rods (13) and two L-shaped inserts (15), the two pressing rods (13) are fixedly connected to the top of the two moving blocks (12), and the two L-shaped inserts (15) are fixedly connected to the bottom of the two moving blocks (12); Spring 2 (14), which is sleeved on the outside of the fixed rod (11); And a limiting component, which is used to fix the sampling tube.

2. The leaf PCR rapid sampling device according to claim 1, characterized in that: The limiting component includes an arc-shaped clamping plate (19), which is fixedly connected to the bottom left side of the placement plate (9). A horizontal plate (21) is fixedly connected to the bottom right side of the placement plate (9). A lead screw (22) is threadedly connected inside the horizontal plate (21). An arc-shaped clamping plate (23) is rotatably connected to the outer left side of the lead screw (22). A handwheel (24) is fixedly connected to the outer right side of the lead screw (22).

3. The leaf PCR rapid sampling device according to claim 1, characterized in that: A protrusion (4) is fixedly connected to the upper left side of the mounting block (1). A handle (5) is rotatably connected to the outside of the protrusion (4). A crossbar (7) is provided inside the connecting block (3). A fixing block (6) is fixedly connected to the top of the crossbar (7). The top of the fixing block (6) is in contact with the handle (5). A sampling head (25) is fixedly connected to the bottom of the crossbar (7). A spring (8) is fixedly connected to the top of the connecting block (3). The spring (8) is sleeved on the outside of the crossbar (7).

4. The leaf PCR rapid sampling device according to claim 1, characterized in that: The connecting block (10) has through holes 1 (16) on both sides of its lower part. The two L-shaped inserts (15) are slidably connected inside the two through holes 1 (16). The connecting block (10) has through holes 2 (18) on both sides of its upper part. The two pressing rods (13) are slidably connected inside the two through holes 2 (18).

5. The leaf PCR rapid sampling device according to claim 3, characterized in that: The connecting block (3) has a sliding hole (26) inside, and the crossbar (7) and the sampling head (25) are slidably connected inside the sliding hole (26).

6. The leaf PCR rapid sampling device according to claim 1, characterized in that: The mounting block (1) has a mounting groove (27) on its outer right side, and the connecting block (10) is inserted into the mounting groove (27).

7. The leaf PCR rapid sampling device according to claim 1, characterized in that: The mounting block (1) has a slot (17) on the right side inside. The two L-shaped plugs (15) are inserted into the two slots (17). The mounting block (1) has a lifting lug (2) fixedly connected to the left side outside.

8. The leaf PCR rapid sampling device according to claim 2, characterized in that: Both the first arc-shaped clamp (19) and the second arc-shaped clamp (23) are provided with protective pads (20), and both protective pads (20) are made of rubber.

Images