Portable constant-temperature fluorescence amplification instrument

Abstract

The utility model relates to biological detection technical field discloses a portable constant temperature fluorescence amplification appearance, including bottom plate, the top of bottom plate is fixedly connected with protection shell, the top of protection shell is fixedly connected with protection cover, the back of protection cover is connected with moving link of slide, the front of moving link is fixedly connected with push block, the front of push block is fixedly connected with two impact blocks, the top of protection cover is connected with placing block of slide, the left and right sides of placing block all are fixedly connected with limit block, the far side of two limit blocks all is fixedly connected with spring no.

Description

Technical Field

[0001] This utility model relates to the field of biological detection technology, and in particular to a portable isothermal fluorescence amplification instrument. Background Technology

[0002] An amplification instrument is an instrument capable of replicating and amplifying specific nucleic acid fragments in biological samples to meet subsequent detection and analysis needs. Isothermal fluorescence amplification instruments, building upon this, employ isothermal amplification technology, avoiding the cumbersome process of repeated temperature increases and decreases required in traditional PCR. They utilize fluorescence signals to monitor the nucleic acid amplification process in real time, enabling qualitative or quantitative analysis of nucleic acids. Portable isothermal fluorescence amplification instruments combine the characteristics of both, offering advantages such as small size and portability. In scenarios such as rapid on-site testing, field operations, and primary healthcare, they can efficiently amplify nucleic acids and monitor fluorescence anytime, anywhere, providing convenient and accurate technical support for biological detection and disease diagnosis.

[0003] The working principle of a portable isothermal fluorescence amplification instrument is to utilize isothermal amplification technology. At a constant temperature, specific primers bind to template nucleic acids, and the target nucleic acid fragments are amplified under the action of DNA polymerase and other agents. At the same time, fluorescently labeled probes are added to the reaction system. When the probes specifically bind to the amplification products, they emit fluorescent signals. The instrument monitors the changes in fluorescence signals in real time to achieve qualitative or quantitative analysis of nucleic acids, thereby quickly and accurately detecting the presence and content of target nucleic acids in samples.

[0004] In existing technologies, the fluorescence of some portable isothermal fluorescence amplifiers cannot be quickly installed, causing the instrument to fail to quickly enter working condition before urgent testing tasks, thus delaying the testing opportunity. To address this issue, a portable isothermal fluorescence amplifier is proposed. Utility Model Content

[0005] To overcome the above shortcomings, this utility model provides a portable isothermal fluorescence amplification instrument, which aims to improve the problem in the prior art that fluorescence cannot be quickly installed, resulting in the instrument not being able to quickly enter the working state before an urgent detection task, thus delaying the detection opportunity.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A portable isothermal fluorescence amplification instrument includes a base plate, a protective shell fixedly connected to the top of the base plate, a protective cover fixedly connected to the top of the protective shell, a movable rod slidably connected to the rear side of the protective cover, a push block fixedly connected to the front side of the movable rod, two impact blocks fixedly connected to the front side of the push block, a placement block slidably connected to the top of the protective cover, limit blocks fixedly connected to both sides of the placement block, a spring fixedly connected to the far side of each of the two limit blocks, a movable plate slidably connected to the outside of the limit blocks, and a heat dissipation component fixedly connected to the rear inner wall of the protective shell.

[0008] As a further description of the above technical solution:

[0009] The heat dissipation assembly includes a filter plate, the front side of which is fixedly connected to the rear side of the protective shell, and a fan is fixedly connected to the inner wall of the rear side of the protective shell.

[0010] As a further description of the above technical solution:

[0011] Two springs are fixedly connected to the rear side of the push block, and the rear sides of the two springs are fixedly connected to the rear inner wall of the protective cover. A spring is fixedly connected to the rear side of the moving plate.

[0012] As a further description of the above technical solution:

[0013] Guide plates are fixedly connected to both the left and right sides of the protective shell. The right side of the movable plate is slidably connected to the left side of the guide plate. A fixing block is fixedly connected to the rear side of the movable rod.

[0014] As a further description of the above technical solution:

[0015] A fixing plate is fixedly connected to the front side of the limiting block, and a rotating plate is rotatably connected to the front side of the fixing plate. The outer side of the rotating plate is rotatably connected to the inner wall of the front side of the protective shell.

[0016] As a further description of the above technical solution:

[0017] A movable block is rotatably connected to the front side of the rotating plate, and the rear side of the movable block contacts the front side of the protective shell under the rotation of the rotating plate.

[0018] As a further description of the above technical solution:

[0019] A placement screen is fixedly connected to the top of the protective shell, a display panel is fixedly connected to the inner wall of the placement screen, a sensor is fixedly connected to the inner wall of the protective shell, and transmission plates are fixedly connected to both sides of the left side of the sensor.

[0020] As a further description of the above technical solution:

[0021] Heating blocks are fixedly connected to the opposite sides of the two transmission plates, a transmission block is fixedly connected to the top of the sensor, multiple placement tubes are fixedly connected to the inner wall of the protective shell, and a movable plate is fixedly connected to the rear side of the placement tube.

[0022] This utility model has the following beneficial effects:

[0023] 1. In this utility model, the movement of the moving rod causes its pushing block to drive the impact block, which in turn causes its moving plate to drive the limiting block into the interior of the placement block, thus fixing the fluorescence module. This achieves rapid installation of the fluorescence module in the portable isothermal fluorescence amplification instrument, and significantly shortens the preparation time of the portable isothermal fluorescence amplification instrument, thereby improving operational efficiency and enabling faster deployment in testing.

[0024] 2. In this utility model, when the temperature is too high during the detection process, the fan brings in cold air from outside the device, which is then filtered by the filter plate. This filters out the particles in the cold air, thus achieving internal heat dissipation for the isothermal fluorescence amplification instrument. In addition, it can effectively maintain a stable temperature environment inside the instrument, ensuring that the fluorescence amplification reaction is carried out at a suitable temperature, thereby improving the accuracy of the detection results. Attached Figure Description

[0025] Figure 1 This is a three-dimensional schematic diagram of a portable isothermal fluorescence amplification instrument proposed in this utility model;

[0026] Figure 2 This is a schematic diagram of the placement tube structure of a portable isothermal fluorescence amplification instrument proposed in this utility model;

[0027] Figure 3 This is a schematic diagram of the fixing block structure of a portable isothermal fluorescence amplification instrument proposed in this utility model;

[0028] Figure 4 This is a schematic diagram of the placement screen structure of a portable isothermal fluorescence amplification instrument proposed in this utility model.

[0029] Legend:

[0030] 1. Base plate; 2. Protective shell; 3. Protective cover; 4. Moving rod; 5. Pushing block; 6. Impact block; 7. Placement block; 8. Limiting block; 9. Spring 1; 10. Moving plate 1; 11. Fan; 12. Filter plate; 13. Fixing plate; 14. Rotating plate; 15. Moving block; 16. Guide plate; 17. Fixing block; 18. Spring 2; 19. Heating block; 20. Placement screen; 21. Display panel; 22. Sensor; 23. Transmission plate; 24. Placement tube; 25. Moving plate 2; 26. Transmission block. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Reference Figure 2 and Figure 3 This utility model provides an embodiment of a portable isothermal fluorescence amplification instrument, comprising a base plate 1, which is the bottom of the entire device and drives its operation. A protective shell 2 is fixedly connected to the top of the base plate 1, protecting the internal heating and testing components. A protective cover 3 is fixedly connected to the top of the protective shell 2, protecting the internal installation and disassembly components. A moving rod 4 is slidably connected to the rear side of the protective cover 3, receiving external force to move. A pushing block 5 is fixedly connected to the front side of the moving rod 4, and two impact blocks 6 are fixedly connected to the front side of the pushing block 5. The pushing block 5 receives the external force from the moving rod 4, thereby driving the impact blocks... The impact block 6 moves, and the top of the protective cover 3 is slidably connected to the placement block 7. The left and right sides of the placement block 7 are fixedly connected to the limit blocks 8. The opposite sides of the two limit blocks 8 are fixedly connected to the springs 9. The outside of the limit blocks 8 is slidably connected to the moving plate 10. The top of the placement block 7 is where the fluorescent module is placed. The limit blocks 8 are fixed to the placement block 7. The springs 9 receive the force of the limit blocks 8 and thus squeeze. The moving plate 10 receives the impact force of the impact block 6 and thus moves the moving plate 10. The rear inner wall of the protective shell 2 is fixedly connected to a heat dissipation component for heat dissipation.

[0033] Reference Figure 2 and Figure 4 The heat dissipation assembly includes a filter plate 12. The front side of the filter plate 12 is fixedly connected to the rear side of the protective shell 2. A fan 11 is fixedly connected to the inner wall of the rear side of the protective shell 2. The fan 11 receives the power transmitted by the device and blows air. The filter plate 12 filters the cold air and removes fine particles.

[0034] Reference Figures 1 to 3Two springs 18 are fixedly connected to the rear side of the push block 5. The rear sides of the two springs 18 are fixedly connected to the rear inner wall of the protective cover 3. The springs 18 receive the pushing force of the push block 5, thereby causing the push block 5 to return to its original position. Guide plates 16 are fixedly connected to both the left and right sides of the protective shell 2. The right side of the moving plate 10 is slidably connected to the left side of the guide plate 16. The guide plate 16 is fixed inside the protective shell 2, so that the movement of the moving plate 10 is linear. The rear of the moving rod 4... A fixed block 17 is fixedly connected to the side, and the fixed block 17 receives external force to move the moving rod 4. A fixed plate 13 is fixedly connected to the front side of the limiting block 8. A rotating plate 14 is rotatably connected to the front side of the fixed plate 13. The outside of the rotating plate 14 is rotatably connected to the front inner wall of the protective shell 2. A moving block 15 is rotatably connected to the front side of the rotating plate 14. The moving block 15 receives external force to make the rotating plate 14 rotate, thereby driving the fixed plate 13 to move, and thus driving the limiting block 8 to move.

[0035] The rear side of the movable block 15 contacts the front side of the protective shell 2 under the rotation of the rotating plate 14. The movable block 15 contacts the protective shell 2, thereby limiting its movement. A placement screen 20 is fixedly connected to the top of the protective shell 2, and a display panel 21 is fixedly connected to the inner wall of the placement screen 20. The placement screen 20 protects the internal display screen, which is equivalent to the control component of the device. A sensor 22 is fixedly connected to the inner wall of the protective cover 3. Transmission plates 23 are fixedly connected to both sides of the left side of the sensor 22. The two transmission plates 23 are fixedly connected to each other on opposite sides. A heating block 19 is fixedly connected to the top of the sensor 22, and a transmission block 26 is fixedly connected to the top of the sensor 22. The sensor 22 receives signals from the device to determine the actual situation of the device. The transmission plate 23 transmits the power of the sensor 22 to the heating block 19, which heats the inside of the device to stabilize it. Multiple placement tubes 24 are fixedly connected to the inner wall of the protective shell 2. The placement tubes 24 are used to place samples. A moving plate 25 is fixedly connected to the rear side of the placement tube 24. The moving plate receives the force applied by the internal sample and thus comes into contact with the sensor 22.

[0036] Working principle: The operator places the placement block 7 inside the protective cover 3. The operator holds the fixed block 17 and moves the moving rod 4. The moving rod 4 moves the pushing block 5, causing the impact block 6 to hit the moving plate 10, which in turn compresses the spring 3. At this time, the moving plate 10 moves linearly under the action of the guide plate 16. Then, the limiting block 8 enters the placement block 7 under the action of the spring 9 and the moving plate 10, thus locking the placement block 7 and realizing the rapid installation of the fluorescent module.

[0037] When the operator turns on the device, sensor 22 receives a signal and heats the heating blocks 19 on both sides under the action of transmission plate 23. After the device reaches a suitable temperature, sensor 22 transmits a signal to the fluorescence module to alert the operator to place the sample into the placement tube 24, thereby moving plate 25 under the action of sensor 22 for detection. When the temperature inside the device is too high, the fan is started to transfer cold air from the outside into the device, thereby dissipating the heat inside the device.

[0038] After the test is completed, the operator pushes the movable block 15, which causes the rotating plate 14 to rotate, thereby causing the fixed plate 13 to rotate and the limiting block 8 to disengage from the placement block 7. This allows for the disassembly of the fluorescent module.

[0039] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A portable isothermal fluorescence amplification instrument, comprising a base plate (1), characterized in that: A protective shell (2) is fixedly connected to the top of the base plate (1), a protective cover (3) is fixedly connected to the top of the protective shell (2), a moving rod (4) is slidably connected to the rear side of the protective cover (3), a pushing block (5) is fixedly connected to the front side of the moving rod (4), two impact blocks (6) are fixedly connected to the front side of the pushing block (5), a placement block (7) is slidably connected to the top of the protective cover (3), a limiting block (8) is fixedly connected to both the left and right sides of the placement block (7), a spring (9) is fixedly connected to the far side of the two limiting blocks (8), a moving plate (10) is slidably connected to the outside of the limiting block (8), and a heat dissipation component for heat dissipation is fixedly connected to the rear inner wall of the protective shell (2).

2. The portable isothermal fluorescence amplification instrument according to claim 1, characterized in that: The heat dissipation assembly includes a filter plate (12), the front side of which is fixedly connected to the rear side of the protective shell (2), and a fan (11) is fixedly connected to the inner wall of the rear side of the protective shell (2).

3. A portable isothermal fluorescence amplification instrument according to claim 1, characterized in that: Two springs (18) are fixedly connected to the rear side of the push block (5), and the rear sides of the two springs (18) are fixedly connected to the rear inner wall of the protective cover (3). A spring (3) is fixedly connected to the rear side of the moving plate (10).

4. A portable isothermal fluorescence amplification instrument according to claim 1, characterized in that: The protective shell (2) is fixedly connected to guide plates (16) on both the left and right sides. The right side of the movable plate (10) is slidably connected to the left side of the guide plate (16). The rear side of the movable rod (4) is fixedly connected to a fixing block (17).

5. A portable isothermal fluorescence amplification instrument according to claim 1, characterized in that: The front side of the limiting block (8) is fixedly connected to a fixing plate (13), and the front side of the fixing plate (13) is rotatably connected to a rotating plate (14). The outside of the rotating plate (14) is rotatably connected to the front inner wall of the protective shell (2).

6. A portable isothermal fluorescence amplification instrument according to claim 5, characterized in that: The front side of the rotating plate (14) is rotatably connected to a movable block (15), and the rear side of the movable block (15) contacts the front side of the protective shell (2) under the rotation of the rotating plate (14).

7. A portable isothermal fluorescence amplification instrument according to claim 1, characterized in that: The top of the protective shell (2) is fixedly connected to a placement screen (20), the inner wall of the placement screen (20) is fixedly connected to a display panel (21), the inner wall of the protective cover (3) is fixedly connected to a sensor (22), and the left sides of the sensor (22) are fixedly connected to transmission plates (23).

8. A portable isothermal fluorescence amplification instrument according to claim 7, characterized in that: Heating blocks (19) are fixedly connected to the opposite sides of the two transmission plates (23), a transmission block (26) is fixedly connected to the top of the sensor (22), a plurality of placement tubes (24) are fixedly connected to the inner wall of the protective shell (2), and a movable plate (25) is fixedly connected to the rear side of the placement tube (24).

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