A constant temperature amplification detector

Abstract

The utility model relates to the field of biotechnology discloses a constant temperature amplification detector, including the bottom plate, the bottom fixedly connected with two limit boards of bottom plate, the outside of two limit boards all are connected with the moving link of sliding, the outside of two moving links all are connected with the fixed shell of sliding, the outside of limit board is connected with the shell of sliding, the similar side of two moving links all is fixedly connected with the fixed ring, the similar side of two fixed rings all is fixedly connected with spring no.

Description

Technical Field

[0001] This utility model relates to the field of biotechnology, and in particular to an isothermal amplification detector. Background Technology

[0002] A detection instrument is a device used to detect specific substances or parameters. The reason why detection instruments employ isothermal amplification is that, compared to traditional temperature-cycled amplification methods, isothermal amplification can rapidly and efficiently amplify target substances such as nucleic acids at a constant temperature, reducing the need for complex temperature control equipment and minimizing operational errors. Isothermal amplification detection instruments are designed and manufactured based on this principle. By precisely maintaining the constant temperature required for amplification, the instrument achieves efficient amplification of nucleic acids in biological samples, thereby enabling the rapid and accurate detection of pathogens such as viruses and bacteria. It plays a crucial role in disease diagnosis, food safety monitoring, and biological research, providing reliable data support for related work.

[0003] The working principle of the isothermal amplification detector is based on specific nucleic acid amplification technology, achieving exponential amplification of the target nucleic acid sequence under constant temperature conditions. It utilizes a polymerase with strand displacement activity, combined with specially designed primers, allowing the amplification reaction to proceed continuously without temperature cycling. When the nucleic acid in the sample binds to the primers, the polymerase extends along the template strand in an isothermal environment to synthesize a new DNA strand, simultaneously displacing the original complementary strand. The displaced single strand can then serve as a new template to trigger a new round of amplification. This cycle repeats, amplifying trace amounts of the target nucleic acid to detectable levels in a short time. The instrument achieves qualitative or quantitative analysis of the nucleic acid by real-time monitoring changes in indicators such as fluorescence signals during the amplification process.

[0004] In existing technologies, some isothermal amplification detectors and their bottom connections are fixed with bolts. During disassembly, the bolts are prone to rusting due to oxidation and corrosion after long-term use, making them difficult to loosen. Forced operation may damage the bolts or the instrument connection. Therefore, an isothermal amplification detector is proposed to solve the above problems. Utility Model Content

[0005] To overcome the above deficiencies, this utility model provides an isothermal amplification detector, which aims to improve the problems in the prior art.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] An isothermal amplification detector includes a base plate. Two limiting plates are fixedly connected to the bottom of the base plate. Movable rods are slidably connected to the outside of each of the two limiting plates. Fixed shells are slidably connected to the outside of each of the two movable rods. Outer shells are slidably connected to the outside of each limiting plate. Fixed rings are fixedly connected to adjacent sides of each of the two movable rods. Springs are fixedly connected to adjacent sides of each of the two fixed rings. A connecting plate is fixedly connected to the front side of each movable rod. A movable block is fixedly connected to the front side of the connecting plate. A force-applying plate is fixedly connected to the front side of the movable block. A closing component for automatically closing the suction port is fixedly connected to the top of the base plate.

[0008] As a further description of the above technical solution:

[0009] The closing assembly includes a detector, the bottom of which is fixedly connected to the top of the base plate. A protective shell is fixedly connected to the inner wall of the detector. A partition is fixedly connected to the inner wall of the protective shell. A sensor is fixedly connected to the front side of the partition. A rotating rod is fixedly connected to the rear side of the sensor. An elliptical axis is fixedly connected to the rear side of the rotating rod. Two force-bearing blocks are slidably connected to the top of the protective shell. A blocking plate is fixedly connected to the top of each of the two force-bearing blocks.

[0010] As a further description of the above technical solution:

[0011] Two guide plates are fixedly connected to the bottom inner wall of the outer casing, and the bottom of the movable block is slidably connected to the top of the guide plates.

[0012] As a further description of the above technical solution:

[0013] The inner wall of the outer shell is fixedly connected to a partition plate two, and the two adjacent sides of the fixed shells are fixedly connected to the left and right sides of the partition plate two.

[0014] As a further description of the above technical solution:

[0015] The outer side of the fixing ring is slidably connected to the inner wall of the fixing shell, and the outer side of the force-applying plate is slidably connected to the front side of the outer shell.

[0016] As a further description of the above technical solution:

[0017] A movable plate is fixedly connected to the front side of both force-bearing blocks, and a guide plate is fixedly connected to the rear side of the partition plate one;

[0018] As a further description of the above technical solution:

[0019] The front side of the movable plate is slidably connected to the rear side of the guide plate 2, and spring 2 is fixedly connected to the opposite side of the two movable plates.

[0020] As a further description of the above technical solution:

[0021] A transfer plate is fixedly connected to both the left and right sides of the sensor, and a heating block is fixedly connected to the opposite side of each of the two transfer plates.

[0022] This utility model has the following beneficial effects:

[0023] 1. In this utility model, the force plate drives the connecting plate to move, thereby causing its moving rod to disengage from the control of the limiting plate, thus causing the spring to be squeezed. After the limiting plate is disengaged, the force applied to the force plate is released, thereby realizing the disassembly of the detector and the connecting parts. In addition, it is convenient to repair or replace the detector or the connecting parts separately, reducing maintenance costs and improving maintenance efficiency.

[0024] 2. In this utility model, the sensor drives the rotating rod to rotate, thereby rotating its elliptical axis, which in turn causes the force-bearing block to receive force, thereby causing the blocking plate to move and opening its opening. This realizes the automatic opening and closing of the detection port. In addition, it can effectively prevent external impurities, dust and other contaminants from entering the instrument, thereby reducing pollution and ensuring the accuracy of detection. Attached Figure Description

[0025] Figure 1 This is a three-dimensional schematic diagram of an isothermal amplification detector proposed in this utility model;

[0026] Figure 2 This is a schematic diagram of the protective shell structure of an isothermal amplification detector proposed in this utility model;

[0027] Figure 3 This is a schematic diagram of the fixed shell structure of an isothermal amplification detector proposed in this utility model;

[0028] Figure 4 This is a schematic diagram of the elliptical axis structure of an isothermal amplification detector proposed in this utility model.

[0029] Legend:

[0030] 1. Base plate; 2. Limiting plate; 3. Moving rod; 4. Fixed shell; 5. Fixed ring; 6. Spring 1; 7. Connecting plate; 8. Moving block; 9. Force-applying plate; 10. Guide plate 1; 11. Detector; 12. Protective shell; 13. Partition 1; 14. Sensor; 15. Rotating rod; 16. Elliptical axis; 17. Force-bearing block; 18. Moving plate; 19. Blocking plate; 20. Spring 2; 21. Guide plate 2; 22. Transmission plate; 23. Heating block; 24. Outer shell; 25. Partition 2. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Reference Figure 2 and Figure 3 This utility model provides an embodiment of an isothermal amplification detector, including a base plate 1, which is the bottom of the detector. Two limiting plates 2 are fixedly connected to the bottom of the base plate 1, thus limiting the detector's position. Moving rods 3 are slidably connected to the outside of each limiting plate 2. The moving rods 3 receive external force and move accordingly, also serving to fix the limiting plates 2. Fixed shells 4 are slidably connected to the outside of each moving rod 3, providing the space for the moving rods 3 to move. The fixed shells 4 contain a spring-loaded component. A shell 24 is slidably connected to the outside of each limiting plate 2, protecting the internal disassembly components from damage. It also serves as a connection point at the bottom. In the connecting part, a fixing ring 5 is fixedly connected to the adjacent side of each of the two moving rods 3. The fixing ring 5 moves with the moving rod 3 and compresses the rebound component. A spring 6 is fixedly connected to the adjacent side of each of the two fixing rings 5. The spring 6 receives the compressive force of the fixing ring 5, stores the elastic force, and returns the elastic force to the fixing ring 5. A connecting plate 7 is fixedly connected to the front side of the moving rod 3. A moving block 8 is fixedly connected to the front side of the connecting plate 7. A force-applying plate 9 is fixedly connected to the front side of the moving block 8. The connecting plate 7 connects the moving block 8 and the moving rod 3. The force-applying plate 9 applies force to the moving block 8, thereby transmitting the force to the top of the bottom plate 1 of the moving rod 3, which is fixedly connected to a closing component for automatically closing the suction port.

[0033] Reference Figure 2 and Figure 4The closing assembly includes a detector 11, the bottom of which is fixedly connected to the top of the base plate 1. The detector 11 is a detector used to detect diseased plants. A protective shell 12 is fixedly connected to the inner wall of the detector 11, protecting the internal automatic closing assembly and sensing assembly. A partition 13 is fixedly connected to the inner wall of the protective shell 12, separating the automatic closing assembly and the sensing assembly. A sensor 14 is fixedly connected to the front side of the partition 13, receiving signals transmitted by the device and heating the device. A rotating rod 15 is fixedly connected to the rear side of the sensor 14. The rotating rod 15 receives the power transmitted by the sensor 14 and rotates accordingly. An elliptical shaft 16 is fixedly connected to the rear side of the rotating rod 15. The elliptical shaft 16 receives the rotational force of the rotating rod 15 and rotates accordingly. Two force-bearing blocks 17 are slidably connected to the top of the protective shell 12. The force-bearing blocks 17 receive the pushing force of the elliptical shaft 16 and move accordingly. A blocking plate 19 is fixedly connected to the top of each of the two force-bearing blocks 17. The blocking plate 19 opens the slot and moves along with the force-bearing blocks 17.

[0034] Reference Figures 1 to 3 Two guide plates 10 are fixedly connected to the bottom inner wall of the outer casing 24. The bottom of the moving block 8 is slidably connected to the top of the guide plate 10. The guide plate 10 allows the moving block 8 to move linearly, thus stabilizing its movement. A partition plate 25 is fixedly connected to the inner wall of the outer casing 24. The adjacent sides of the two fixed casings 4 are fixedly connected to the left and right sides of the partition plate 25. The partition plate 25 provides a fixed place for the fixed casings 4 and also separates the two disassembled parts. The outer side of the fixing ring 5 is slidably connected to the inner wall of the fixed casing 4. The fixing ring 5 receives the pushing force of the moving rod 3, thus moving within the fixed casing 4. The outer side of the force plate 9 is slidably connected to the front side of the outer casing 24. The force plate 9 receives external force, thus moving on the front side of the outer casing 24, driving the moving block 8 to move. The front sides of the two force blocks 17 are fixed. A movable plate 18 is connected, which receives the pushing force from the force block 17 and moves accordingly. A guide plate 21 is fixedly connected to the rear side of the partition 13, and the front side of the movable plate 18 is slidably connected to the rear side of the guide plate 21. The guide plate 21 is fixed on the partition, so that the movable plate 18 can move linearly. A spring 20 is fixedly connected to the opposite side of the two movable plates 18. The spring 20 receives the pushing force of the movable plate 18 and stores the elastic force, so that the spring 20 returns the elastic force to the movable plate 18. A transmission plate 22 is fixedly connected to both sides of the sensor 14. The transmission plate 22 receives the power of the sensor 14 and transmits it to the heating component. A heating block 23 is fixedly connected to the opposite side of the two transmission plates 22. The heating block 23 receives the power transmitted by the transmission plate 22 and heats the equipment.

[0035] Working principle: The operator holds the force plate 9, which moves the moving block 8. Under the action of the guide plate 10, the moving block 8 moves linearly, which in turn moves the moving rod 3. The moving rod 3 then moves the fixing ring 5, which in turn compresses the spring 6. At this point, the moving rod 3 is released from the control of the limit plate 2, causing the base plate 1 to detach from the outer shell 24. The operator releases the control of the force plate 9, the spring 6 releases its force, and the moving rod 3 moves back to its original position. This allows for the disassembly of the detector and connecting parts. In addition, it facilitates the individual repair and replacement of the detector or connecting parts, reducing maintenance costs and improving maintenance efficiency.

[0036] When testing is required, the operator turns on the equipment. Sensor 14 receives a signal, which, under the action of the transfer plate 22, preheats the two heating blocks 23 to the appropriate temperature. When sensor 14 detects that the equipment has reached the required temperature, it causes the rotating rod 15 to rotate, thereby driving the elliptical shaft 16 to rotate. The rotation of the elliptical shaft 16 causes the force-bearing blocks 17 on both sides to receive a pushing force and move, thereby driving the top blocking plate 19 to move, and also driving the moving plate 18 to move, causing the spring 20 to be compressed. The top slot is opened, and the negative pressure device at the bottom moves, reducing the internal pressure and allowing external air to enter the device, causing it to contact the partition. After a certain period, the rotating rod 15 reverses, and the force block 17 is no longer restricted by the pushing force, causing the spring 20 to release its force, which moves the blocking plate 19 and closes the slot. This achieves automatic opening and closing of the detection port, and effectively prevents external impurities and dust from entering the instrument, thereby reducing contamination and ensuring the accuracy of the detection.

[0037] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An isothermal amplification detector, comprising a base plate (1), characterized in that: The bottom of the base plate (1) is fixedly connected to two limiting plates (2), and the outside of the two limiting plates (2) is slidably connected to a moving rod (3). The outside of the two moving rods (3) is slidably connected to a fixed shell (4). The outside of the limiting plate (2) is slidably connected to a shell (24). The adjacent sides of the two moving rods (3) are fixedly connected to a fixing ring (5). The adjacent sides of the two fixing rings (5) are fixedly connected to a spring (6). The front side of the moving rod (3) is fixedly connected to a connecting plate (7). The front side of the connecting plate (7) is fixedly connected to a moving block (8). The front side of the moving block (8) is fixedly connected to a force-applying plate (9). The top of the base plate (1) is fixedly connected to a closing component for automatically closing the suction port.

2. The isothermal amplification detector according to claim 1, characterized in that: The closing assembly includes a detector (11), the bottom of which is fixedly connected to the top of the base plate (1). A protective shell (12) is fixedly connected to the inner wall of the detector (11). A partition (13) is fixedly connected to the inner wall of the protective shell (12). A sensor (14) is fixedly connected to the front side of the partition (13). A rotating rod (15) is fixedly connected to the rear side of the sensor (14). An elliptical axis (16) is fixedly connected to the rear side of the rotating rod (15). Two force-bearing blocks (17) are slidably connected to the top of the protective shell (12). A blocking plate (19) is fixedly connected to the top of each of the two force-bearing blocks (17).

3. The isothermal amplification detector according to claim 1, characterized in that: The bottom inner wall of the outer shell (24) is fixedly connected to two guide plates (10), and the bottom of the moving block (8) is slidably connected to the top of the guide plates (10).

4. The isothermal amplification detector according to claim 1, characterized in that: The inner wall of the outer shell (24) is fixedly connected to a partition plate two (25), and the two fixed shells (4) are fixedly connected to the left and right sides of the partition plate two (25) on their adjacent sides.

5. The isothermal amplification detector according to claim 1, characterized in that: The outer side of the fixing ring (5) is slidably connected to the inner wall of the fixing shell (4), and the outer side of the force-applying plate (9) is slidably connected to the front side of the outer shell (24).

6. The isothermal amplification detector according to claim 2, characterized in that: The front sides of the two force-bearing blocks (17) are fixedly connected to a movable plate (18), and the rear side of the partition plate (13) is fixedly connected to a guide plate (21).

7. The isothermal amplification detector according to claim 6, characterized in that: The front side of the movable plate (18) is slidably connected to the rear side of the guide plate (21), and springs (20) are fixedly connected to the opposite sides of the two movable plates (18).

8. The isothermal amplification detector according to claim 2, characterized in that: The sensor (14) is fixedly connected to a transmission plate (22) on both the left and right sides, and a heating block (23) is fixedly connected to the opposite side of the two transmission plates (22).

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