An enzyme-linked immunospot image analyzer

Abstract

The utility model relates to image analysis technical field, especially in kind enzyme -linked immunoassay spot image analysis appearance, including bottom plate and setting at bottom plate top's shell, the top of bottom plate is used for placing enzyme -linked plate, is provided with XY movement module on bottom plate, XY movement module is connected with the main camera for shooting enzyme -linked plate on linkage, and monocular light source lens is connected on main camera, XY movement module is provided with photoelectric component for detecting main camera position, the bottom plate is provided with support frame on main camera one side corresponding, support frame is provided with global camera for shooting enzyme -linked plate on, both ends of support frame all and bottom plate present detachable state connection, and two fast lock components are set up on bottom plate, two fast lock components are respectively set up on the door in the opening or closing state on the shell middle side facade respectively, through above -mentioned technical scheme, the utility model solves the problem that the present image analysis appearance shooting angle exists limit, the low and maintenance efficiency problem of low shooting efficiency.

Description

Technical Field

[0001] This utility model relates to an image analyzer, specifically an enzyme-linked immunosorbent assay (ELISA) dot image analyzer. Background Technology

[0002] An ELISPOT Reader is a device used to analyze the results of ELISPOT (Enzyme-Linked Immunospot) assays. ELISPOT technology combines cell culture and enzyme-linked immunosorbent assay (ELISA) techniques, enabling the detection of cytokines or antibodies secreted by cells at the single-cell level. This technology uses specific antibodies to capture proteins secreted by cells, and then forms visible spots through an enzymatic reaction, thus achieving highly sensitive detection of cellular function.

[0003] However, existing enzyme-linked immunosorbent assay (ELISA) spot image analyzers typically have only one camera. They first take a global image of the ELISA plate with this camera, and then adjust the position of the camera to take a specific image of a certain location on the ELISA plate as needed. This not only limits the shooting angle of the image, but also leads to low shooting efficiency, which affects the accuracy of subsequent analysis results and prolongs the analysis cycle. Furthermore, with the long-term use of the ELISA dot image analyzer, regular maintenance of its internal structures is required. Existing ELISA dot image analyzers typically have only one door. When maintaining the ELISA dot image analyzer, the components on the side closer to the door are easier to maintain, while the components on the side farther from the door are more difficult to maintain, thus affecting maintenance efficiency. Utility Model Content

[0004] In order to solve the problems in related technologies, this utility model provides an enzyme-linked immunosorbent assay (ELISA) dot image analyzer, which solves the problems of limited shooting angle, low shooting efficiency and low maintenance efficiency of existing image analyzers.

[0005] To solve the above problems, the following technical solutions are provided: An enzyme-linked immunosorbent assay (ELISA) dot image analyzer includes a base plate and a housing disposed on top of the base plate. The top of the base plate is used to place an ELISA plate. An XY motion module is disposed on the base plate. The main camera for photographing the ELISA plate is linked to the XY motion module, and a monocular light source lens is connected to the main camera. A photoelectric component for detecting the position of the main camera is disposed on the XY motion module. A support frame is disposed on the base plate on one side of the main camera. A global camera for photographing the ELISA plate is disposed on the support frame. Both ends of the support frame are detachably connected to the base plate. The base plate is provided with two quick-locking components for fixing or releasing the support frame. Doors in an open or closed state are respectively disposed on the side surfaces of the housing corresponding to the two quick-locking components.

[0006] Through the above technical solution, by setting up a global camera and a main camera, the microplate can be photographed first through the global camera, and then the position of the main camera can be adjusted according to the shooting needs to take detailed pictures of a certain position on the microplate. The combined shooting of the global camera and the main camera can not only capture pictures of the microplate from all angles, but also effectively improve the shooting efficiency, thereby improving the accuracy of subsequent analysis results and shortening the cycle of obtaining analysis results. Furthermore, the two doors allow for easy removal of the support frame from the base plate when the ELISA dot image analyzer requires maintenance. This is achieved by opening both doors and removing the support frame from the base plate from either side. The structure is simple and easy to operate. After the support frame is removed, the disassembly of other components, such as the XY motion module, is also easy. Reinstalling the components after maintenance is also easy, thus reducing maintenance difficulty and improving maintenance efficiency.

[0007] Furthermore, the quick-lock assembly includes clamping pliers, and the clamping pliers are disposed on one side of the support frame; The support frame includes a first connecting plate and two identical mounting plates. The first connecting plate is U-shaped, and the horizontal portions of the first connecting plate are spaced apart above the base plate. The two mounting plates are respectively fixedly connected to two vertical portions of the first connecting plate. The two clamping clamps are used to clamp or loosen the corresponding mounting plates, so that the support frame is in a fixed or free state. The global camera is mounted on the horizontal part of the first connecting plate, and a first ring light source is provided at the bottom of the horizontal part of the first connecting plate.

[0008] Through the above technical solution, the clamping clamp not only provides a strong clamping force, which ensures that the support frame will not move or loosen after it is fixed, but also ensures the installation stability of the global camera and the first ring light source on the support frame, thereby ensuring the stable use of the enzyme-linked immunosorbent assay (ELISA) dot image analyzer. Moreover, the clamping clamp is very easy to operate; simply turning the handle can achieve clamping or loosening, thereby improving work efficiency. By setting up the first ring light source, uniform illumination can be provided, making the light distribution on the surface of the ELISA plate more uniform. This reduces shadows and reflections in the photos taken by the global camera, which is beneficial for subsequent image processing and analysis, thereby improving the accuracy of the analysis results.

[0009] Furthermore, the XY motion module includes two identical first moving components and a second moving component. The two first moving components are respectively disposed at both ends of the width direction in the base plate. The enzyme label plate is disposed on the top of the base plate between the two first moving components. The second moving component is disposed along the length direction of the base plate, and the two ends of the second moving component in the length direction are respectively connected to the two first moving components in a linked manner. The main camera and the second moving component are connected in a linked manner.

[0010] Through the above technical solution, by setting up the XY motion module, the main camera can not only move along the length of the base plate, but also move along the width of the base plate, thereby enabling the main camera to capture images of any position on the ELISA plate or even each well, thus improving the accuracy of subsequent structural analysis.

[0011] Furthermore, each of the first moving components includes a first fixed seat and a first sliding seat. Each first fixed seat is arranged along the width direction of the base plate. Each first fixed seat is provided with a first ball screw. Each first ball screw is arranged along the length direction of the corresponding first fixed seat. Both ends of the length direction of each first ball screw are rotatably connected to the corresponding first fixed seat. Each first sliding seat is threadedly connected to the corresponding first ball screw. One end of the length direction of each first fixed seat is provided with a first motor. Each first motor is fixedly connected to the corresponding first ball screw. The second moving component includes a second fixed seat and a second sliding seat. The two ends of the second fixed seat in the length direction are respectively fixedly connected to the two first sliding seats. A second ball screw is provided on the second fixed seat. The second ball screw is arranged along the length direction of the second fixed seat, and both ends of the second ball screw in the length direction are rotatably connected to the second fixed seat. The second sliding seat and the second ball screw are threadedly connected. A second motor is fixedly provided at one end of the second fixed seat in the length direction. The output shaft of the second motor is fixedly connected to the second ball screw. A second connecting plate is fixedly mounted on the second sliding seat, the main camera is mounted on the other end of the second connecting plate, and a second ring light source is mounted on the bottom of the second connecting plate.

[0012] By using the above technical solution, the friction between the first sliding seat and the first ball screw, as well as the second sliding seat and the second ball screw, can be reduced through the setting of the first ball screw and the second ball screw. This can improve the movement accuracy of the first sliding seat and the second sliding seat, thereby ensuring the accurate adjustment of the main camera position. At this time, the main camera can accurately capture the images of the wells required on the ELISA plate, thereby improving efficiency.

[0013] Furthermore, the photoelectric component includes three photoelectric sensors respectively disposed on the top of the first motor and the two second motors. Each photoelectric sensor has the same structure and is a reflective photoelectric sensor.

[0014] Through the above technical solution, by setting up three reflective photoelectric sensors, the reflective photoelectric sensors can detect minute changes in reflected light, which can accurately adjust the position of the main camera, thereby ensuring the accuracy of image acquisition and the accuracy of subsequent analysis results.

[0015] Furthermore, it also includes multiple support feet with identical structures, which are evenly arranged along the bottom periphery of the base plate, and each support foot is provided with an anti-slip pad at its bottom.

[0016] The above technical solution, through the setting of multiple support feet, can raise the height of the base plate. When it is necessary to move the ELISA dot image analyzer, the gap between the raised base plate and the bottom of the support feet facilitates the entry of a robotic arm or forklift, thereby improving the efficiency of moving the ELISA dot image analyzer. The setting of anti-slip pads can increase the friction between the support feet and the ground, thereby ensuring the stability of the ELISA dot image analyzer.

[0017] Furthermore, a limiting plate is provided at the top of the end of the base plate that is away from the first motor in the width direction, and a notch is provided at the bottom of one side of the outer casing where there is no door, and the shape of the notch is consistent with that of the limiting plate.

[0018] The above solution has the following advantages: 1. By setting up the global camera and the main camera, the microplate can be photographed first using the global camera, and then the position of the main camera can be adjusted according to the shooting needs to take detailed pictures of a certain position on the microplate. The combined shooting of the global camera and the main camera can not only capture pictures of the microplate from all angles, but also effectively improve the shooting efficiency, thereby improving the accuracy of subsequent analysis results and shortening the cycle of obtaining analysis results. In addition, with the two doors, when the enzyme-linked immunosorbent assay (ELISA) image analyzer needs maintenance, the two doors can be opened separately. At this time, the two ends of the support frame can be removed from the base plate from the two doors respectively. The structure is simple and easy to operate. After the support frame is removed, the disassembly of other components such as the XY motion module is also easy. When the components are reinstalled after maintenance, it is also easy to operate, thereby reducing the difficulty of maintenance and improving the efficiency of maintenance. 2. The clamping clamp provides strong clamping force, ensuring the support frame remains fixed and secure, thus guaranteeing the stability of the global camera and the first ring light source on the support frame and ensuring the stable operation of the ELISA dot image analyzer. Furthermore, the clamping clamp is very easy to operate; simply turning the handle allows for clamping or loosening, improving work efficiency. The first ring light source provides uniform illumination, resulting in a more even light distribution on the surface of the ELISA plate, reducing shadows and reflections in the photos taken by the global camera. This facilitates subsequent image processing and analysis, thereby improving the accuracy of the analysis results. 3. By setting up the XY motion module, the main camera can not only move along the length of the base plate, but also move along the width of the base plate, thereby enabling the main camera to capture images of any position on the ELISA plate or even each well, thus improving the accuracy of subsequent structural analysis. 4. By setting the first ball screw and the second ball screw, the friction between the first sliding seat and the first ball screw, the second sliding seat and the second ball screw can be reduced. This can improve the movement accuracy of the first sliding seat and the second sliding seat, thereby ensuring the accurate adjustment of the main camera position. At this time, the main camera can accurately capture the image of the well required on the ELISA plate, thereby improving efficiency. 5. By setting up three reflective photoelectric sensors, the reflective photoelectric sensors can detect minute changes in reflected light, which can accurately adjust the position of the main camera, thereby ensuring the accuracy of image acquisition and the accuracy of subsequent analysis results. 6. The multiple support feet raise the base plate, allowing for easier access for robotic arms or forklifts when the ELISA dot image analyzer needs to be moved. The anti-slip pads increase friction between the support feet and the ground, ensuring the stability of the ELISA dot image analyzer. Attached Figure Description

[0019] To make the content of this utility model easier to understand, the present utility model will be further described in detail below with reference to specific embodiments and accompanying drawings, wherein: Figure 1 This is a schematic diagram of the structure of an enzyme-linked immunosorbent assay (ELISA) dot image analyzer. Figure 2 This is a schematic diagram of a shell-less structure in an enzyme-linked immunosorbent assay (ELISA) dot image analyzer. Figure 3 This is a schematic diagram of the fast-lock component in an enzyme-linked immunosorbent assay (ELISA) dot image analyzer during rotation. Figure 4 for Figure 2 A magnified view of part number A in the middle; Explanation of reference numerals in the attached drawings: 1. Base plate; 2. Outer shell; 3. Microplate; 4. Main camera; 5. Optoelectronic component; 6. Support frame; 601. First connecting plate; 602. Mounting plate; 7. Global camera; 8. Quick-lock assembly; 9. Door; 10. First ring light source; 11. First moving component; 1101. First fixed seat; 1102. First sliding seat; 12. Second moving component; 1201. Second fixed seat; 1202. Second sliding seat; 13. First ball screw; 14. First motor; 15. Second ball screw; 16. Second motor; 17. Second connecting plate; 18. Second ring light source; 19. Support foot. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] In a specific embodiment, such as Figures 1-4 As shown, an enzyme-linked immunosorbent assay (ELISA) dot image analyzer includes a base plate and a housing disposed on top of the base plate. The top of the base plate is used to place an ELISA plate. An XY motion module is disposed on the base plate, and a main camera for photographing the ELISA plate is linked to the XY motion module. The main camera is connected to a monocular light source lens. In this specific embodiment, the main camera is a CCD camera, and the image captured by the CCD camera has a resolution of 2448*2048 pixels. An optoelectronic component for detecting the position of the main camera is disposed on the XY motion module. A support frame is disposed on the base plate on one side of the main camera. A global camera for photographing the ELISA plate is disposed on the support frame, and the image captured by the global camera has a resolution of 1920*1200 pixels. Both ends of the support frame are detachably connected to the base plate, and two quick-lock components are disposed on the base plate for fixing or releasing the support frame. Doors in open or closed states are respectively disposed on the side surfaces of the housing corresponding to the two quick-lock components.

[0022] The microplate can be photographed first using a global camera, and then the position of the main camera can be adjusted according to the shooting needs to take detailed pictures of a certain position on the microplate. The combination of the global camera and the main camera can not only capture pictures of the microplate from all angles, but also effectively improve the shooting efficiency, thereby improving the accuracy of subsequent analysis results and shortening the cycle of obtaining analysis results. When the ELISA dot image analyzer needs maintenance, open both doors and remove the two ends of the support frame from the base plate from the direction of each door. The structure is simple and easy to operate. After the support frame is removed, the disassembly of other components such as the XY motion module is also easy. When the components are reinstalled after maintenance, it is also easy to operate, thereby reducing the difficulty of maintenance and improving the efficiency of maintenance.

[0023] The quick-lock assembly includes clamping pliers, which are located on one side of the support frame. The structure and specific installation of the clamping pliers are existing technologies and will not be described in detail here. The support frame includes a first connecting plate and two mounting plates with identical structures. The first connecting plate is U-shaped, and the horizontal portions of the first connecting plate are spaced apart above the base plate. The two mounting plates are fixedly connected to the two vertical portions of the first connecting plate, respectively. The two clamping pliers are used to clamp or loosen the corresponding mounting plates, so that the support frame is in a fixed or free state. A global camera is set on the horizontal part of the first connecting plate, and a first ring light source is set at the bottom of the horizontal part of the first connecting plate. The clamping clamp not only provides strong clamping force, ensuring that the support frame will not move or loosen after it is fixed, thus ensuring the installation stability of the global camera and the first ring light source on the support frame, and thus ensuring the stable use of the ELISA dot image analyzer, but also makes the operation of the clamping clamp very simple. Just turn the handle to perform the clamping or loosening operation, thereby improving work efficiency. By setting up the first ring light source, uniform illumination can be provided, making the light distribution on the surface of the ELISA plate more uniform. This reduces shadows and reflections in the photos taken by the global camera, which is beneficial for subsequent image processing and analysis, thereby improving the accuracy of the analysis results.

[0024] The XY motion module includes two identical first moving components and one second moving component. The two first moving components are respectively located at both ends of the base plate in the width direction. The ELISA plate is located on top of the base plate between the two first moving components. The second moving component is located along the length direction of the base plate, and its two ends in the length direction are respectively connected to the two first moving components in a linkage manner. The main camera and the second moving component are also linked. The XY motion module can not only drive the main camera to move along the length direction of the base plate, but also drive the main camera to move along the width direction of the base plate, thereby enabling the main camera to capture images of any position on the ELISA plate or even each well, thus improving the accuracy of subsequent structural analysis.

[0025] Each first moving component includes a first fixed seat and a first sliding seat. Each first fixed seat is arranged along the width direction of the base plate. Each first fixed seat is provided with a first ball screw. Each first ball screw is arranged along the length direction of the corresponding first fixed seat. Both ends of the length direction of each first ball screw are rotatably connected to the corresponding first fixed seat. Each first sliding seat is threadedly connected to the corresponding first ball screw. One end of the length direction of each first fixed seat is provided with a first motor. Each first motor is fixedly connected to the corresponding first ball screw. The second moving component includes a second fixed seat and a second sliding seat. The two ends of the second fixed seat in the length direction are respectively fixedly connected to two first sliding seats. A second ball screw is provided on the second fixed seat. The second ball screw is arranged along the length direction of the second fixed seat, and both ends of the second ball screw in the length direction are rotatably connected to the second fixed seat. The second sliding seat and the second ball screw are threadedly connected. A second motor is fixedly provided at one end of the second fixed seat in the length direction. The output shaft of the second motor is fixedly connected to the second ball screw. A second connecting plate is fixedly installed on the second sliding seat, the main camera is installed on the other end of the second connecting plate, and a second ring light source is installed at the bottom of the second connecting plate; In this specific embodiment, it is common knowledge to use two ring lamps to provide the first ring light source and the second ring light source, so it will not be described in detail here; the ring lamps are connected to wires, and the other end of the wires is provided with a three-hole connector. The other end of the three-hole connector passes through the outer shell, and the operator provides power to the ring lamps by inserting the adapted three-hole connector into the three-hole connector. By setting the first ball screw and the second ball screw, the friction between the first sliding seat and the first ball screw, the second sliding seat and the second ball screw can be reduced. This can improve the movement accuracy of the first sliding seat and the second sliding seat, thereby ensuring the accurate adjustment of the main camera position. At this time, the main camera can accurately capture the image of the well required on the ELISA plate, thereby improving efficiency.

[0026] The optoelectronic component includes three optoelectronic sensors respectively mounted on top of the first motor and two second motors. Each optoelectronic sensor has the same structure and is a reflective optoelectronic sensor. The reflective optoelectronic sensor can detect minute changes in reflected light, which allows for precise adjustment of the position of the main camera, thereby ensuring the accuracy of image acquisition and the accuracy of subsequent analysis results.

[0027] An enzyme-linked immunosorbent assay (ELISA) dot image analyzer also includes multiple support feet with identical structures. The multiple support feet are evenly arranged along the bottom periphery of the base plate. The multiple support feet can raise the height of the base plate. When the ELISA dot image analyzer needs to be moved, the gap between the raised base plate and the bottom of the support feet facilitates the entry of a robotic arm or forklift, thereby improving the efficiency of moving the ELISA dot image analyzer. Each support foot is equipped with an anti-slip pad on its bottom. The anti-slip pad can increase the friction between the support foot and the ground, thereby ensuring the stability of the enzyme-linked immunosorbent assay (ELISA) dot image analyzer.

[0028] A limiting plate is provided at the top of the end of the base plate that is far from the first motor in the width direction. A notch is provided at the bottom of one side of the outer casing where there is no door, and the shape of the notch is consistent with that of the limiting plate.

[0029] In practical use, it is common knowledge that there is analysis software on the host computer used to analyze photos taken by the global camera and the main camera, so it will not be elaborated on here. The analysis software can not only obtain photos taken by the global camera and the main camera respectively, but also control the opening or closing of the ring light source and the second ring light source, or the brightness of the light source, as well as control the operation of the first motor and the second motor. These are all existing technologies, so they will not be elaborated on here.

[0030] Operation process: Open any door, place the ELISA plate on the base plate. The position and orientation of the ELISA plate are not limited. After turning on the first ring light source, the global camera will take pictures of the ELISA plate. After taking pictures, the images will be sent to the analysis software to analyze the precise position of each well and identify the ELISA plate information. Then, the analysis software will control the main camera to move to the position of each well of the ELISA plate to take pictures. The analysis software will analyze the acquired images and output an analysis report. Open any door again, take out the ELISA plate, and the analysis will end. When the ELISA dot image analyzer is not in operation and requires maintenance, open both doors. At this time, the clamping clamps can be rotated to detach from the mounting plates at both ends of the support frame. The support frame, the first ring light source, and the global camera can then be removed for maintenance. The main camera, the second ring light source, and other components inside the housing can also be maintained. After all maintenance is completed, the support frame containing the second ring light source and the global camera should be placed back into the housing. The clamping clamps should be rotated to firmly press the support frame. After installation, close both doors.

[0031] In the description of this utility model, it should be understood that the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In the description of this utility model, unless otherwise specified and limited, it should be noted that the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to mechanical or electrical connections, or internal connections between two components, and can be direct connections or indirect connections through an intermediate medium. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0032] Obviously, the above embodiments are merely examples for clear illustration and are not intended to limit the implementation. For those skilled in the art, other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all implementation methods here, and any obvious variations or modifications derived therefrom are still within the protection scope of this utility model.

Claims

1. An enzyme-linked immunosorbent assay (ELISA) dot image analyzer, comprising a base plate and a housing disposed on top of the base plate, wherein the top of the base plate is used to place an ELISA plate, and an XY motion module is disposed on the base plate, characterized in that, The XY motion module is linked to a main camera for photographing the ELISA plate, and the main camera is connected to a monocular light source lens. The XY motion module is equipped with a photoelectric component for detecting the position of the main camera. A support frame is provided on the base plate corresponding to one side of the main camera. A global camera for photographing the ELISA plate is provided on the support frame. Both ends of the support frame are detachably connected to the base plate. The base plate is provided with two quick-lock components for fixing or releasing the support frame. The two quick-lock components are respectively provided with doors in the open or closed state on the side surface of the outer shell.

2. The enzyme-linked immunosorbent assay (ELISA) dot image analyzer as described in claim 1, characterized in that, The quick-lock assembly includes clamping pliers, which are disposed on one side of the support frame; The support frame includes a first connecting plate and two mounting plates with identical structures. The first connecting plate is U-shaped, and the horizontal portions of the first connecting plate are spaced apart above the base plate. The two mounting plates are respectively fixedly connected to two vertical portions of the first connecting plate. The two clamping clamps are used to clamp or loosen the corresponding mounting plates, so that the support frame is in a fixed or free state. The global camera is mounted on the horizontal part of the first connecting plate, and a first ring light source is provided at the bottom of the horizontal part of the first connecting plate.

3. The enzyme-linked immunosorbent assay (ELISA) dot image analyzer as described in claim 1, characterized in that, The XY motion module includes two identical first moving components and a second moving component. The two first moving components are respectively disposed at both ends of the width direction of the base plate. The enzyme label plate is disposed on the top of the base plate between the two first moving components. The second moving component is disposed along the length direction of the base plate, and the two ends of the second moving component in the length direction are respectively connected to the two first moving components in a linked manner. The main camera and the second moving component are connected in a linked manner.

4. The enzyme-linked immunosorbent assay (ELISA) dot image analyzer as described in claim 3, characterized in that, Each of the first moving components includes a first fixed seat and a first sliding seat. Each first fixed seat is arranged along the width direction of the base plate. Each first fixed seat is provided with a first ball screw. Each first ball screw is arranged along the length direction of the corresponding first fixed seat. Both ends of the length direction of each first ball screw are rotatably connected to the corresponding first fixed seat. Each first sliding seat is threadedly connected to the corresponding first ball screw. One end of the length direction of each first fixed seat is provided with a first motor. Each first motor is fixedly connected to the corresponding first ball screw.

5. The enzyme-linked immunosorbent assay (ELISA) dot image analyzer as described in claim 4, characterized in that, The second moving component includes a second fixed seat and a second sliding seat. The two ends of the second fixed seat along its length are respectively fixedly connected to the two first sliding seats. A second ball screw is provided on the second fixed seat. The second ball screw is arranged along the length of the second fixed seat, and both ends of the second ball screw along its length are rotatably connected to the second fixed seat. The second sliding seat and the second ball screw are threadedly connected. A second motor is fixedly provided at one end of the second fixed seat along its length, and the output shaft of the second motor is fixedly connected to the second ball screw.

6. The enzyme-linked immunosorbent assay (ELISA) dot image analyzer as described in claim 5, characterized in that, A second connecting plate is fixedly mounted on the second sliding seat, the main camera is mounted on the other end of the second connecting plate, and a second ring light source is mounted on the bottom of the second connecting plate.

7. The enzyme-linked immunosorbent assay (ELISA) dot image analyzer as described in claim 5, characterized in that, The optoelectronic component includes three optoelectronic sensors respectively disposed on the top of the first motor and the two second motors. Each optoelectronic sensor has the same structure and is a reflective optoelectronic sensor.

8. The enzyme-linked immunosorbent assay (ELISA) dot image analyzer as described in claim 1, characterized in that, It also includes multiple support feet with the same structure, which are evenly arranged along the bottom periphery of the base plate, and each support foot is provided with an anti-slip pad at the bottom.

Images