A manual quantitative bacteria injector for ralstonia solanacearum

Abstract

This utility model belongs to the field of Solanaceae bacterial wilt pathogens, specifically a manual quantitative injection device for these pathogens. Addressing the problem that existing syringes have relatively simple structures and manual control makes it difficult to ensure the accuracy and uniformity of quantitative injection, the following solution is proposed: It includes a syringe and a mounting block installed on one side of the syringe. Two symmetrically arranged positioning blocks are fixedly mounted on one side of the mounting block. A positioning groove is formed on one side of each positioning block. A circular hole is formed through the surface of the mounting block, and a clearance opening is formed on one side of the circular hole, communicating with the circular hole. This utility model ensures the accuracy and uniformity of quantitative injection. Furthermore, it allows for the prefabrication of limiting blocks and adjusting rods with different spacing specifications, enabling the use of suitable parts. The detachable design of the device facilitates placement and allows for compatibility with disposable or reusable syringes of the same specifications, making it flexible and convenient to use.

Description

Technical Field

[0001] This utility model relates to the field of bacterial wilt pathogens of the Solanaceae family, and in particular to a manual quantitative inoculum injector for bacterial wilt pathogens of the Solanaceae family. Background Technology

[0002] Bacterial wilt of Solanaceae is the pathogen that causes bacterial wilt in Solanaceae crops (such as eggplant, tomato, and pepper). Its scientific name is *Raulella solanaceae*, and it is a bacterial soil-borne disease. It can invade the vascular bundles through wounds in the roots or stem base, multiply within the xylem vessels, and block water transport channels, leading to plant wilting.

[0003] In the study of bacterial wilt pathogens of the Solanaceae family, it is often necessary to use syringes to inject the pathogens in a quantitative manner. However, traditional syringes have a relatively simple structure, and manual control makes it difficult to ensure the accuracy and uniformity of quantitative injection.

[0004] Therefore, we propose a manual quantitative inoculum injector for the Solanaceae bacterial wilt pathogen. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies where the syringe structure is relatively simple and manual control makes it difficult to ensure the accuracy and uniformity of quantitative injection. Therefore, this invention proposes a manual quantitative injection device for Ralstonia solanacearum, the pathogen of the Solanaceae family.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A manual quantitative inoculum injector for *Ralstonia solanaceae*, the causal agent of bacterial wilt, comprises:

[0008] The syringe and the mounting block installed on one side of the syringe. Two symmetrically arranged positioning blocks are fixedly installed on one side of the mounting block. A positioning groove is opened on one side of the positioning block. A circular hole is opened through the surface of the mounting block. A clearance opening is opened on one side of the circular hole. The clearance opening communicates with the circular hole. A connecting groove perpendicular to the axial direction of the circular hole is opened between the circular hole and the clearance opening.

[0009] An adjusting rod is inserted into a round hole. Multiple evenly distributed limiting blocks are fixedly installed on the outer wall of the adjusting rod. The limiting blocks are adapted to the clearance opening and the connecting groove. One of the limiting blocks is located in the connecting groove. A stop block is fixedly installed at the top of the adjusting rod.

[0010] In this process, the limiting block is moved from the connecting groove into the relief port by rotating the adjusting rod. After moving the adjusting rod along the axial direction of the circular hole, it is rotated to embed another limiting block into the connecting groove, thereby setting the position of the stop block. The push block stops when it contacts the stop block, thus realizing quantitative injection.

[0011] In one possible design, the syringe includes a syringe barrel with two symmetrically arranged flanges integrally formed on the outer wall of the top end of the syringe barrel. The positioning groove cooperates with the flanges. A piston is slidably connected inside the syringe barrel. A push rod is fixedly disposed on the top end of the piston. A push block is fixedly disposed on the top end of the push rod. The push block contacts a stop block to limit the stroke of the push block.

[0012] In one possible design, rubber pads are adhered to both the top and bottom inner walls of the connecting groove.

[0013] In one possible design, the mounting block includes a first connecting block and a second connecting block, which are bonded together to form the mounting block.

[0014] In one possible design, the mounting block is made of transparent PVC, and the adjusting rod, limiting block, and stop block are made of aluminum alloy.

[0015] In one possible design, a snap fastener made of TPEE is fixed on one side of the mounting block.

[0016] In this application, during use, an appropriate amount of sample is drawn using a syringe, then the needle is fitted onto the front end, and the mounting block is attached to one side of the syringe, allowing the flange to enter the positioning groove for positioning. This causes the snap-fit ​​to deform and fit onto the outer wall of the syringe, preventing the mounting block from shifting. Then, the push block, push rod, and piston are pushed to move, squeezing out the air inside the needle. Simultaneously, the push block presses against the stop block. Then, the stop block adjustment rod is rotated 90 degrees, causing the limiting block to disengage from the connecting groove and enter the clearance port. Then, the adjustment rod is pressed down, placing the adjacent limiting block into the clearance port and rotating it 90 degrees in the opposite direction, causing the limiting block to enter the connecting groove. Under the action of the rubber pad, the adjustment rod and stop block remain stable and do not shift. Then, the needle is placed into the inoculation container, and the push block, push rod, and piston are pushed to move, injecting the bacterial culture into the container until the push block presses against the stop block again, completing the quantitative injection of the bacterial culture. The next injection operation is the same, thereby ensuring the uniformity of the injections.

[0017] Beneficial effects: The manual quantitative injection device for Solanaceae bacterial wilt fungus described in this utility model can ensure the accuracy and uniformity of quantitative injection through the setting of structures such as adjusting rod, limiting block and stop block, and limiting blocks and adjusting rods with different spacing specifications can be prefabricated, and appropriate parts can be used when in use;

[0018] In this utility model, the manual quantitative inoculation device for Solanaceae bacterial wilt pathogens, through the setting of structures such as mounting blocks, adjusting rods and positioning blocks, the detachable setting of the device can not only be placed conveniently, but also be adapted to disposable syringes or reusable syringes of the same specification, making it flexible and convenient to use;

[0019] In this utility model, the manual quantitative inoculum injector for the Solanaceae bacterial wilt pathogen has a simple structure of each component, which is suitable for mass production, through the setting of adjustment rod, mounting block and buckle, etc.

[0020] This invention ensures the accuracy and uniformity of quantitative injection, and allows for the prefabrication of limiting blocks and adjusting rods with different spacing specifications. Appropriate parts can be used during operation. The detachable design of the equipment facilitates placement and allows for compatibility with disposable or reusable syringes of the same specifications. It is flexible and convenient to use, and the simple structure of each component makes it suitable for mass production. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall three-dimensional structure of a manual quantitative inoculum injector for the Solanaceae bacterial wilt pathogen proposed in this utility model;

[0022] Figure 2 This is a schematic diagram of the overall exploded structure of a manual quantitative inoculum injector for the Solanaceae bacterial wilt pathogen proposed in this utility model;

[0023] Figure 3 This is a partially exploded structural diagram of a manual quantitative inoculum injector for the Solanaceae bacterial wilt pathogen proposed in this utility model;

[0024] Figure 4 This is a partial cross-sectional view of a manual quantitative inoculum injector for the Solanaceae bacterial wilt pathogen proposed in this utility model.

[0025] In the diagram: 1. Syringe; 101. Needle barrel; 102. Flange; 103. Piston; 104. Push rod; 105. Push block; 2. Mounting block; 201. First connecting block; 202. Second connecting block; 3. Adjusting rod; 4. Limiting block; 5. Stop block; 6. Buckle; 7. Positioning block; 8. Positioning groove; 9. Clearance opening; 10. Round hole; 11. Connecting groove; 12. Rubber pad. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0027] In one embodiment: Refer to Figures 1-4A manual quantitative bacterial injection device includes: a syringe 1 and a mounting block 2. The mounting block 2 is mounted on one side of the syringe 1, and two symmetrically distributed positioning blocks 7 are provided on the side of the mounting block 2 near the syringe 1. Positioning grooves 8 are formed on the surface of the positioning blocks 7 for positioning the syringe 1. A circular hole 10 is formed through the middle of the mounting block 2, and one side of the circular hole 10 is connected to a relief port 9. The circular hole 10 and the relief port 9 are connected by a connecting groove 11. An adjusting rod 3 passes through the circular hole 10, and multiple equally spaced limiting blocks 4 are fixed to its outer wall. The size of the limiting blocks 4 matches the size of the relief port 9 and the connecting groove 11. In the initial state, at least one limiting block 4 is embedded in the connecting groove 11. The top end of the adjusting rod 3 extends to form a stop block 5.

[0028] The syringe 1 consists of a syringe barrel 101, a piston 103, a plunger 104, and a push block 105. Two flanges 102 are symmetrically formed on the outer wall of the top of the syringe barrel 101. During assembly, the flanges 102 engage with the positioning groove 8 for lateral positioning. A TPEE material clip 6 is also provided on one side of the mounting block 2. During assembly, the clip 6 elastically deforms and engages with the outer wall of the syringe barrel 101, preventing longitudinal displacement of the mounting block 2. The piston 103 slides and seals within the syringe barrel 101. One end of the plunger 104 fixes the piston 103, and the other end connects to the push block 105. The end of the push block 105's stroke contacts the stop block 5, forming a mechanical limit point for the injection volume.

[0029] This application can be used in the field of bacterial wilt of Solanaceae family, or in other fields applicable to this application.

[0030] In another embodiment: a manual quantitative inoculum injector for Solanaceae bacterial wilt pathogens, which is applied to the field of Solanaceae bacterial wilt pathogens;

[0031] In another aspect of this embodiment, the mounting block 2 is formed by bonding a first connecting block 201 and a second connecting block 202 made of transparent PVC material, facilitating observation of the internal limiting block 4. The adjusting rod 3, the limiting block 4, and the stop block 5 are made of lightweight aluminum alloy. The TPEE material of the buckle 6 provides durable elasticity and adapts to repeated disassembly and assembly. All components can be produced using standardized molds, suitable for mass production.

[0032] Operating mechanism of the regulating mechanism

[0033] In the initial state, the limiting block 4 embedded in the connecting groove 11 is fixed by friction through the rubber pad 12 adhered to the inner wall of the connecting groove 11. When the injection volume needs to be adjusted:

[0034] Rotate the adjusting rod 3 by about 90 degrees to disengage the limiting block 4 from the connecting groove 11 and into the relief port 9;

[0035] Move the adjusting rod 3 along the axial direction of the circular hole 10, and the target limiting block 4 slides into the clearance port 9;

[0036] Rotate the adjusting rod 3 in the opposite direction by about 90 degrees, and the target limiting block 4 is embedded in the connecting groove 11. The friction of the rubber pad 12 maintains the stability of the position of the adjusting rod 3.

[0037] The spacing of the limit blocks 4 determines the length of a single injection stroke. Different injection volume requirements can be adapted by using prefabricated adjustment rods 3 with different spacing specifications.

[0038] Workflow:

[0039] a. After extracting the bacterial solution, align the mounting block 2 with the flange 102 through the positioning groove 8, and lock the syringe 101 with the buckle 6;

[0040] b. Push the pusher block 105 to expel air until the pusher block 105 contacts the stop block 5;

[0041] c. Adjust the position of the limiting block 4 according to the above steps to set the injection stroke;

[0042] d. After inserting the inoculation container, push the pusher 105. The pusher 105 stops when it touches the stop block 5 again, thus completing the quantitative injection.

[0043] e. During repeated operations, the contact position between the stop block 5 and the push block 105 remains constant to ensure consistent injection dosage across multiple injections.

[0044] The accompanying drawings in this application are for illustrative purposes only. The dimensions and shapes of the components shown are not actual limitations but are merely schematic representations. In actual implementation, the components can be reasonably configured and adjusted according to specific needs and actual conditions.

[0045] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A manual quantitative inoculum injector for *Ralstonia solanaceae*, characterized in that, include: The syringe (1) and the mounting block (2) installed on one side of the syringe (1) are provided. Two symmetrically arranged positioning blocks (7) are fixedly provided on one side of the mounting block (2). A positioning groove (8) is provided on one side of the positioning block (7). A circular hole (10) is provided through the surface of the mounting block (2). A relief opening (9) is provided on one side of the circular hole (10). The relief opening (9) is connected to the circular hole (10). A connecting groove (11) perpendicular to the axis of the circular hole (10) is provided between the circular hole (10) and the relief opening (9). An adjusting rod (3) is inserted into a round hole (10). Multiple evenly distributed limiting blocks (4) are fixedly installed on the outer wall of the adjusting rod (3). The limiting blocks (4) are adapted to the clearance opening (9) and the connecting groove (11). One of the limiting blocks (4) is located in the connecting groove (11). A stop block (5) is fixedly installed at the top of the adjusting rod (3). In this process, by rotating the adjusting rod (3), the limiting block (4) is moved from the connecting groove (11) into the relief port (9). After the adjusting rod (3) is moved axially along the circular hole (10), it is rotated to embed another limiting block (4) into the connecting groove (11), thereby setting the position of the stop block (5). The push block (105) stops when it touches the stop block (5), thus realizing quantitative injection.

2. The manual quantitative inoculum injector for *Ralstonia solanaceae* according to claim 1, characterized in that, The syringe (1) includes a syringe (101). The top outer wall of the syringe (101) is integrally formed with two symmetrically arranged flanges (102). The positioning groove (8) cooperates with the flanges (102). A piston (103) is slidably connected inside the syringe (101). A push rod (104) is fixedly provided at the top of the piston (103). A push block (105) is fixedly provided at the top of the push rod (104). The push block (105) contacts the stop block (5) to limit the stroke of the push block (105).

3. A manual quantitative inoculum injector for *Ralstonia solanaceae* wilt pathogen according to claim 2, characterized in that, Rubber pads (12) are adhered to the top inner wall and the bottom inner wall of the connecting groove (11).

4. A manual quantitative inoculum injector for *Ralstonia solanaceae* according to claim 3, characterized in that, The mounting block (2) includes a first connecting block (201) and a second connecting block (202), which are bonded together to form the mounting block (2).

5. A manual quantitative inoculum injector for *Ralstonia solanaceae* wilt pathogen according to claim 4, characterized in that, The mounting block (2) is made of transparent PVC, and the adjusting rod (3), limiting block (4) and stop block (5) are made of aluminum alloy.

6. A manual quantitative inoculum injector for *Ralstonia solanaceae* according to claim 5, characterized in that, The mounting block (2) has a buckle (6) fixed on one side, which is made of TPEE.

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