An isolation cover for studying diseases of Sichuan pepper fruit

Abstract

This utility model discloses an isolation cover for studying diseases of Sichuan pepper fruit, including a frustum-shaped support cover (1). The upper opening (11) of the support cover (1) seals and wraps the Sichuan pepper branches to be studied, and the fruit granules at the top of the Sichuan pepper fruit to be studied are completely wrapped inside the support cover (1). A detachable moisturizing component (2) is provided at the lower opening (11) of the support cover (1). The moisturizing component (2) is used to absorb water and keep moist, and the moisturizing component (2) completely covers the lower opening (11). A plastic bag (3) is placed over the support cover (1). This utility model solves the problems in the prior art, where the moisturizing method of directly covering with degreased cotton or sponge is difficult to operate, the surface of the Sichuan pepper fruit cannot reach the moisturizing conditions after inoculation, the fruit is easy to fall off and prematurely aging, and repeated inoculation is required to complete the pathogen infection test, resulting in a low inoculation success rate.

Description

Technical Field

[0001] This utility model relates to the field of agricultural production technology, and in particular to an isolation cover for studying diseases of Sichuan pepper fruits. Background Technology

[0002] During the cultivation of Sichuan pepper, various diseases can occur on the fruit. Therefore, identifying the causative agents of these diseases and implementing targeted control measures is crucial. The identification of pathogens causing Sichuan pepper fruit diseases requires isolation, purification, re-inoculation, and re-isolation experiments. Due to the small size of Sichuan pepper fruits, the pathogens cannot be kept moist after inoculation, making re-inoculation experiments difficult to succeed and severely impacting the identification of pathogens. Therefore, an isolation shield is needed to prevent the pathogens from infecting the fruit, verify Koch's postulates on Sichuan pepper fruit diseases, and accurately identify the pathogens causing these diseases.

[0003] There are two common methods for pathogen reinoculation: one is inoculation using mycelial blocks, and the other is inoculation using spore suspension. When using Sichuan pepper fruits as the inoculation target, it is difficult to successfully induce disease in the fruits, regardless of whether the inoculation is performed with or without damage. Individual Sichuan pepper fruits are small, and the entire fruit is umbrella-shaped, making the conventional method of covering with absorbent cotton or sponges for moisture retention difficult to implement. This hinders the normal growth of the Sichuan pepper, and the fruits fall off prematurely before any disease symptoms appear after inoculation, making it difficult to verify Koch's postulates.

[0004] Existing technology 1:

[0005] Mycelial block inoculation: Using a 3mm diameter punch, cut mycelial fragments from the edge of the colony and place the mycelial side onto the surface of the inoculated plant. Use sterile PDA agar discs as a control. After re-inoculation, cover with sterile absorbent cotton or sponge, and place the plant in a plastic bag. Incubate under constant humidity for 48-72 hours, observing for disease development. If the inoculated plant develops disease, remove the affected area and re-isolate to verify Koch's postulates.

[0006] Spore suspension inoculation: Pathogen spores were washed from PDA plates with sterile water to prepare a spore suspension with a concentration of 10⁷ spores / ml. The suspension was then applied to the surface of the inoculated plant using a brush or sprayer, with sterile water as a control. After re-inoculation, the plants were covered with plastic bags and incubated under constant humidity for 48-72 hours, observing for disease development. If the inoculated plant developed symptoms, the affected areas were removed and re-isolated to verify Koch's postulates.

[0007] Disadvantages of existing technology 1:

[0008] 1. Existing technology cannot enable the pepper fruit to grow normally, which can easily cause the pepper fruit to fall off and age prematurely.

[0009] 2. The fruit of Sichuan pepper is small and umbrella-shaped. Existing inoculation methods cannot achieve the desired moisture retention effect, making it difficult for pathogens to infect the plant and resulting in a low inoculation success rate. Summary of the Invention

[0010] The purpose of this invention is to provide an isolation cover for studying diseases of Sichuan pepper fruits. It solves the problems in the existing technology, such as the difficulty in operating the method of directly covering with degreased cotton or sponge to retain moisture, the inability of the Sichuan pepper fruits to reach the required moisture level after inoculation, the easy drop and premature aging of the fruits, the need for repeated inoculation to complete the pathogen infection test, and the low success rate of inoculation.

[0011] This utility model provides the following technical solution:

[0012] An isolation cover for studying diseases of Sichuan pepper fruits includes a frustum-shaped support cover. The upper opening of the support cover is sealed to enclose the branches of the Sichuan pepper to be studied, and the fruit berries at the top of the Sichuan pepper fruits to be studied are completely enclosed inside the support cover. A detachable moisturizing element is provided at the lower opening of the support cover. The moisturizing element is used to absorb water and keep moist, and the moisturizing element completely covers the lower opening. A plastic bag is placed over the support cover.

[0013] Preferably, the material used to manufacture the support cover is a fan-shaped single-layer white pollination bag or a single-layer white fruit bag.

[0014] Preferably, the material used to manufacture the moisturizing component is self-sterilizing degreased cotton or sterilized sponge.

[0015] Preferably, the moisturizing element is circular in shape, and the diameter of the moisturizing element is larger than the diameter of the lower opening.

[0016] Preferably, the diameter of the upper opening is equal to the diameter of the pepper branch to be studied, and it is fixed to the pepper branch to be studied by tape; the shape of the support cover is frustum, and the height of the support cover is greater than the height of the fruit at the top of the pepper; the diameter of the lower opening is greater than the lateral length of the fruit at the top of the pepper.

[0017] Preferably, the height of the food preservation bag is greater than the height of the support cover; the lateral length of the food preservation bag is greater than the diameter of the lower opening of the support cover.

[0018] Preferably, the moisturizing element is secured to the lower opening by a paperclip or clip.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. This utility model provides an isolation cover for studying diseases of Sichuan pepper fruits. Under conditions that maintain the natural growth of the Sichuan pepper, a support cover is set outside the Sichuan pepper fruits. The diameter of the opening of the support cover is determined by the diameter of the top branch of the inoculated Sichuan pepper tree, the height of the support cover is determined by the maximum longitudinal length of the top fruit of the inoculated Sichuan pepper tree, and the diameter of the bottom opening of the support cover is determined by the maximum transverse length of the top fruit of the inoculated Sichuan pepper tree. This device can be flexibly made to fit the actual situation of the Sichuan pepper fruits. Under natural inoculation conditions, it can maintain the shape of the Sichuan pepper fruits, has light transmission and support functions, and ensures the normal growth of the inoculated Sichuan pepper fruits.

[0021] 2. This utility model is equipped with a moisture-retaining component. After the support cover is fixed to the top branch, sterilized degreased cotton is used to cover the end of the support cover and fix it. The support cover is covered with a plastic bag, which provides better moisture-retaining conditions after inoculation, can prevent interference from external pests or strong winds, and the process is easy to operate with a high inoculation success rate. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the pepper isolation cover of this utility model.

[0023] Reference numerals: 1-Support cover; 11-Upper opening; 12-Lower opening; 2-Moisturizing component; 3-Preservation bag. Detailed Implementation

[0024] The present invention will now be described in detail with reference to the embodiments and accompanying drawings. However, it should be understood that the embodiments and accompanying drawings are for illustrative purposes only and do not constitute any limitation on the scope of protection of the present invention. All reasonable modifications and combinations that fall within the scope of the inventive spirit of the present invention are within the scope of protection of the present invention.

[0025] The present invention will be further described below with reference to the accompanying drawings.

[0026] An isolation cover for studying diseases of Sichuan pepper fruit includes a frustum-shaped support cover 1. The upper opening 11 of the support cover 1 seals and wraps the Sichuan pepper branches to be studied for the diseases of the fruit, and the fruit berries at the top of the Sichuan pepper to be studied are completely wrapped inside the support cover 1. A detachable moisturizing component 2 is provided at the lower opening 11 of the support cover 1. The moisturizing component 2 is used to absorb water and keep moist, and the moisturizing component 2 completely covers the lower opening 11. A plastic bag 3 is placed over the support cover 1.

[0027] The material of the support cover 1 in this embodiment is selected from a fan-shaped single-layer white pollination bag or a single-layer white fruit bag.

[0028] In this embodiment, the moisturizing component 2 is selected from sterilized degreased cotton or sterilized sponge, with a thickness of 5cm or more.

[0029] In this embodiment, the moisturizing component 2 is circular, with a diameter at least 2 cm larger than the diameter of the lower opening 12.

[0030] In this embodiment, the diameter of the upper opening 11 is equal to the diameter of the pepper branch to be studied, and it is fixed to the pepper branch with tape; the support cover 1 is frustum-shaped, and the height of the frustum is at least 1-2 cm greater than the longitudinal length of the fruit at the top of the pepper to be studied; the diameter of the lower opening 12 is at least 1-2 cm greater than the transverse length of the fruit at the top of the pepper to be studied.

[0031] In this embodiment, the height of the food preservation bag 3 is at least 5cm greater than the height of the support cover 1; the lateral length of the food preservation bag 3 is at least 10cm greater than the diameter of the lower opening 12 of the support cover 1.

[0032] In this embodiment, the moisturizing component 2 is fixed to the lower opening 1 by a paperclip or clip.

[0033] When this implementation plan is implemented,

[0034] like Figure 1 This embodiment provides an isolation cover for studying diseases of Sichuan pepper fruits, hereinafter referred to as the Sichuan pepper isolation cover. It includes a frustum-shaped support cover 1. The diameter of the upper opening 11 of the Sichuan pepper branches and the support cover 1 is the same. A moisture-retaining element 2 is provided at the lower opening 12 of the support cover 1. The moisture-retaining element 2 has the same shape as the lower opening 12, completely covering it. The Sichuan pepper to be studied is wrapped inside the support cover 1 and secured by the sealed upper opening 11 and the moisture-retaining element 2 at the bottom.

[0035] The material used to manufacture the support cover 1 is selected from single-layer white pollination bags or single-layer white fruit bags. After inoculation, it provides support and light transmission, maintains the shape of the pepper berries, and ensures the normal growth of the pepper fruit. A fan-shaped single-layer white pollination bag is used to wrap the apical berries of the pepper tree to be studied. The upper opening 11 is fixed with adhesive tape at the connection point, forming a frustum-shaped or funnel-shaped support cover 1. The diameter of the upper opening 11 of the support cover 1 is equal to the diameter of the pepper branch and is fixed with tape during use. The support cover 1 is frustum-shaped, and the height of the frustum is determined by the longitudinal length of the apical berries of the inoculated pepper tree, being 1-2 cm greater than this length, ensuring that the moisture-retaining component 2 does not contact the pepper. The diameter of the lower opening 12 of the frustum is determined by the transverse length of the apical berries of the inoculated pepper tree, being 1-2 cm greater than this length, so as not to affect the normal growth of the pepper.

[0036] The moisturizing component 2 is selected from sterilized absorbent cotton or sterilized sponge, is cylindrical in shape, has a thickness of more than 5cm, and its diameter exceeds the diameter of the lower opening 12 by at least 2cm. During use, the edge of the moisturizing component 2 is fixed to the edge of the lower opening 12. The purpose of the moisturizing component 2 is to achieve a moisturizing effect without affecting the growth of the pepper fruit. It is fixed to the edge of the lower opening 12 with a paperclip or clip, making it easy to remove and reinstall, which is beneficial for daily moisturizing management and observation of disease development after inoculation.

[0037] To increase the duration of moisturizing, a preservation bag 3 is provided outside the support cover 1. The height of the preservation bag 3 should be at least 5 cm greater than the height of the support cover 1, and the horizontal length of the preservation bag 3 should be at least 10 cm greater than the diameter of the lower opening 12 of the support cover 1, so as to avoid affecting the growth of Sichuan pepper.

[0038] Spraying sterilized water into the plastic bag 2-3 times a day can provide better moisture conditions after inoculation, prevent interference from external pests or strong winds, and the process is easy to operate with a high success rate of inoculation.

[0039] The above embodiments are merely preferred embodiments of this utility model, and the protection scope of this utility model is not limited to the above embodiments. All technical solutions falling within the scope of this utility model's concept are within its protection scope. It should be noted that improvements and modifications made by those skilled in the art without departing from the principle of this utility model should also be considered within its protection scope.

Claims

1. An isolation cover for studying diseases of Sichuan pepper fruits, characterized in that: The support includes a frustum-shaped support cover (1), the upper opening (11) of which seals and wraps the pepper branches to be studied, and the fruit granules at the top of the pepper fruit to be studied are completely wrapped inside the support cover (1); a detachable moisturizing component (2) is provided at the lower opening (12) of the support cover (1), the moisturizing component (2) is used to absorb water and keep moist, and the moisturizing component (2) completely covers the lower opening (12); a plastic bag (3) is placed over the support cover (1).

2. The isolation cover for studying diseases of Sichuan pepper fruit according to claim 1, characterized in that: The material used to manufacture the support cover (1) is a fan-shaped single-layer white pollination bag or a single-layer white fruit bag.

3. The isolation cover for studying diseases of Sichuan pepper fruit according to claim 1, characterized in that: The material used to manufacture the moisturizing component (2) is self-sterilizing degreased cotton or sterilized sponge.

4. The isolation cover for studying diseases of Sichuan pepper fruit according to claim 3 or 1, characterized in that: The moisturizing element (2) is circular in shape, and the diameter of the moisturizing element (2) is larger than the diameter of the lower opening (12).

5. The isolation cover for studying diseases of Sichuan pepper fruit according to claim 1, characterized in that: The diameter of the upper opening (11) is equal to the diameter of the pepper branch to be studied, and it is fixed to the pepper branch to be studied by tape; the shape of the support cover (1) is frustum-shaped, and the height of the support cover (1) is greater than the height of the fruit at the top of the pepper to be studied; the diameter of the lower opening (12) is greater than the lateral length of the fruit at the top of the pepper to be studied.

6. The isolation cover for studying diseases of Sichuan pepper fruit according to claim 1, characterized in that: The height of the preservation bag (3) is greater than the height of the support cover (1); the lateral length of the preservation bag (3) is greater than the diameter of the lower opening (12) of the support cover (1).

7. The isolation cover for studying diseases of Sichuan pepper fruit according to claim 1, characterized in that: The moisturizing element (2) is fixed to the lower opening (12) by a paperclip or clip.

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