Plant callus cleaning and sterilization device

Abstract

The utility model relates to plant tissue culture technical field provides a kind of plant callus cleaning and sterilizing device, including base and the processing box being located on base, the processing box is equipped with reciprocating movable loading plate, multiple unit cells for placing plant callus are arranged on the loading plate, and each unit cell bottom is equipped with several through holes, the cleaning and sterilizing device further includes the spray assembly for the plant callus after soaking and sterilizing is washed with sterile water;The plant callus to be cleaned and sterilized is placed in unit cell in turn, then appropriate sterilization liquid is injected into processing box to soak and sterilize for specified time length, in soaking and sterilizing process, loading plate reciprocating movement makes the plant callus placed in unit cell be in continuous shaking state, and reciprocating movement loading plate will promote sterilization liquid flow, so that sterilization liquid fully penetrates and contacts each part of plant callus, guarantee the comprehensiveness and effectiveness of sterilization.

Description

Technical Field

[0001] This utility model relates to the field of plant tissue culture technology, specifically a device for cleaning and sterilizing plant callus tissue. Background Technology

[0002] In plant tissue culture, cleaning and sterilization are crucial steps that directly affect the success rate of subsequent cultures. Traditional methods for cleaning and sterilizing plant callus involve immersing the callus in a suitable bactericidal solution for a period of time to achieve cleaning and sterilization. After soaking, the callus is rinsed with sterile water to remove any residual bactericidal solution. However, in traditional methods, the plant callus remains relatively quiescent in the bactericidal solution. This quiescent state means that certain parts of the callus, especially complex, uneven, or hard-to-reach areas, cannot fully contact the bactericidal solution. The bactericidal solution cannot effectively penetrate these hard-to-reach areas, thus greatly weakening the comprehensiveness and effectiveness of cleaning and sterilization. This may leave potential sources of microbial contamination, affecting subsequent plant tissue cultures and even causing the entire culture process to fail due to incomplete cleaning and sterilization.

[0003] Therefore, this utility model proposes a plant callus cleaning and sterilization device to solve the above problems. Utility Model Content

[0004] The purpose of this utility model is to provide a plant callus cleaning and sterilization device to solve the above-mentioned problems.

[0005] To achieve the above objectives, this utility model provides the following technical solution: A plant callus cleaning and sterilization device includes a base and a processing box disposed on the base. The processing box is provided with a reciprocating loading plate. Multiple cell units for placing plant callus are arranged on the loading plate, and each cell unit has several through holes at the bottom. The cleaning and sterilization device also includes a spray assembly for rinsing the plant callus after soaking and sterilization with sterile water.

[0006] In one alternative: the inner wall of each cell is covered with a soft silicone layer.

[0007] In one alternative embodiment: the inner walls of the processing box are provided with horizontally oriented sliding grooves on opposite sides, the two sides of the loading plate are respectively engaged in the corresponding sliding grooves, the loading plate is provided with a first boss and a second boss, a plurality of elastic reset members are provided between the first boss and the inner wall of the processing box, the processing box is provided with a rotatable flower-shaped component, and the side of the flower-shaped component abuts against the second boss, and the processing box is also provided with a driving member for driving the flower-shaped component to rotate.

[0008] In one alternative: the upper side of the treatment box is open, and the spray assembly includes a detachable spray plate located at the opening of the treatment box and a delivery pipe for conveying sterile water into the spray plate, wherein spray holes are evenly distributed at the bottom of the spray plate.

[0009] In one alternative: the processing tank is divided into an immersion chamber and a storage chamber by a partition. The loading plate is located in the immersion chamber. A discharge port is provided on one side of the immersion chamber, and a first sealing valve is provided at the discharge port. An injection port is provided on the partition, and a second sealing valve is provided at the injection port. A sealing piston is provided in the storage chamber. A telescopic component is provided at the bottom of the storage chamber, and the telescopic end of the telescopic component is connected to the sealing piston.

[0010] Compared with the prior art, the beneficial effects of this utility model embodiment are as follows: The plant callus tissue to be cleaned and sterilized is placed sequentially in the cells, with one cell corresponding to one plant callus tissue. Then, an appropriate amount of sterilizing solution is injected into the treatment box for soaking and sterilization for a specified period of time. During the soaking and sterilization process, the carrier plate moves back and forth, causing the plant callus tissue placed in the cell to be in a continuous shaking state. The back and forth movement of the carrier plate also promotes the flow of the sterilizing solution, allowing the sterilizing solution to fully penetrate and contact all parts of the plant callus tissue, ensuring the comprehensiveness and effectiveness of sterilization.

[0011] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application. Attached Figure Description

[0012] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this application and, together with the description, serve to explain the principles of this application. Furthermore, these drawings and textual descriptions are not intended to limit the scope of the concept in any way, but rather to illustrate the concepts of this application to those skilled in the art through reference to specific embodiments.

[0013] Figure 1 This is a schematic diagram of the structure of this utility model.

[0014] Figure 2 This is a schematic diagram of the structure of the carrier plate in an embodiment of this utility model.

[0015] Figure 3 This is a schematic diagram of the carrier plate from another angle in an embodiment of this utility model.

[0016] Figure 4 for Figure 1 Enlarged view of point A in the middle.

[0017] Figure 5 for Figure 1 Enlarged view of section B in the middle.

[0018] Figure 6 This is a three-dimensional schematic diagram of the flower-shaped component in an embodiment of this utility model.

[0019] Figure reference numerals: 1-base, 2-processing box, 201-immersion chamber, 202-liquid storage chamber, 3-spray assembly, 301-spray plate, 302-spray hole, 303-transfer pipe, 4-discharge port, 5-first sealing valve, 6-injection port, 7-second sealing valve, 8-sealing piston, 9-telescopic component, 10-carrying plate, 11-cell unit, 12-through hole, 13-slide groove, 14-first boss, 15-elastic reset component, 16-second boss, 17-floral component. Detailed Implementation

[0020] The present application will now be described in further detail with reference to the accompanying drawings. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0021] Please see Figures 1-3 A plant callus cleaning and sterilization device includes a base 1 and a processing box 2 disposed on the base 1. The processing box 2 is provided with a reciprocatingly movable loading plate 10. Multiple cell units 11 for placing plant callus are arranged on the loading plate 10, and each cell unit 11 has several through holes 12 at the bottom. The cleaning and sterilization device also includes a spray assembly 3 for rinsing the plant callus after soaking and sterilization with sterile water.

[0022] The plant callus tissue to be cleaned and sterilized is placed sequentially in cell 11, with one cell 11 corresponding to one plant callus tissue (to avoid damage caused by collisions when the plant callus tissue shakes). Then, an appropriate amount of sterilizing solution is injected into the treatment tank 2 (completely immersing cell 11) for a specified soaking and sterilization time. During the soaking and sterilization process, the placement plate 10 moves back and forth, causing the plant callus tissue placed in cell 11 to be in a continuous shaking state. The back and forth movement of the placement plate 10 promotes the flow of the sterilizing solution, allowing the sterilizing solution to fully penetrate and contact all parts of the plant callus tissue, ensuring comprehensive and effective sterilization. After the specified soaking and sterilization time is reached, the sterilizing solution in the treatment tank 2 is discharged. Then, sterile water is sprayed onto the plant callus tissue through the spray assembly 3 to rinse away the residual sterilizing solution (it should be noted that during the process of rinsing the plant callus tissue with sterile water, the placement plate 10 is also in a back and forth movement state). Thus, the cleaning and sterilization of the plant callus tissue is completed.

[0023] Furthermore, each of the cell 11 has a soft silicone layer (not shown in the figure) covering its inner wall, which serves to protect the plant callus tissue and prevent it from being damaged by bumping against the inner wall of the cell 11 when it shakes.

[0024] Please see Figures 4-6 In one embodiment of this utility model, the inner walls of the processing box 2 are provided with horizontally oriented sliding grooves 13 on opposite sides. The two sides of the loading plate 10 are respectively engaged in the corresponding sliding grooves 13. The loading plate 10 is provided with a first boss 14 and a second boss 16. A plurality of elastic reset members 15 are provided between the first boss 14 and the inner wall of the processing box 2 (the elastic reset members 15 are springs, sheet metal, etc. in the prior art, as long as they can achieve the elastic reset function, they are not limited here). The processing box 2 is provided with a rotatable flower-shaped member 17, and the side of the flower-shaped member 17 abuts against the second boss 16. The processing box 2 is also provided with a driving member for driving the flower-shaped member 17 to rotate (not shown in the figure, the driving member is a servo motor, geared motor, etc. in the prior art, and is not limited or described here).

[0025] In this embodiment, the flower-shaped component 17 is driven to rotate by the driving component. When the flower-shaped component 17 rotates, its side will abut against the second protrusion 16, causing the carrier plate 10 to slide towards the elastic reset component 15, thereby compressing the elastic reset component 15. As the flower-shaped component 17 continues to rotate, it will "restore" the carrier plate 10. The carrier plate 10 is reset under the action of the elastic reset component 15. This cycle is repeated to realize the reciprocating movement of the carrier plate 10.

[0026] Please see Figure 1 In one embodiment of the present invention, the upper side of the treatment box 2 is open, and the spray assembly 3 includes a detachable spray plate 301 disposed at the opening of the treatment box 2 and a conveying pipe 303 for conveying sterile water into the spray plate 301. Spray holes 302 are evenly distributed at the bottom of the spray plate 301.

[0027] In this embodiment, sterile water is supplied externally and transported to the spray tray 301 through the delivery pipe 303. Then, it is sprayed onto the plant callus tissue through the spray holes 302 to rinse it and remove any residual bactericidal solution. It should be noted that the spray tray 301 can be detached using common existing technologies such as snaps, bolts, and slots. This embodiment does not limit this. During cleaning and sterilization, the plant callus tissue is placed first, and then the spray tray 301 is installed to enable subsequent spray rinsing. After cleaning and sterilization are completed, the spray tray 301 is removed to take out the plant callus tissue.

[0028] Please see Figure 1In one embodiment of this utility model, the processing box 2 is divided into an immersion chamber 201 and a storage chamber 202 by a partition. The loading plate 10 is disposed in the immersion chamber 201. A discharge port 4 is provided on one side of the immersion chamber 201, and a first sealing valve 5 is provided at the discharge port 4. A liquid injection port 6 is provided on the partition, and a second sealing valve 7 is provided at the liquid injection port 6. A sealing piston 8 is provided in the storage chamber 202. A telescopic component 9 is provided at the bottom of the storage chamber 202 (the telescopic component 9 can be a telescopic cylinder, electric telescopic rod, etc. in the prior art, and is not limited here), and the telescopic end of the telescopic component 9 is connected to the sealing piston 8.

[0029] In this embodiment, the storage chamber 202 is used to store the disinfectant solution. When the disinfectant solution needs to be added to the soaking chamber 201, the first sealing valve 5 is kept closed and the second sealing valve 7 is opened. The telescopic member 9 extends and pushes the sealing piston 8 upward, so that the disinfectant solution in the storage chamber 202 enters the soaking chamber 201 through the injection port 6. When a certain amount is reached, the telescopic member 9 stops extending and the second sealing valve 7 is closed. After the disinfection is used up, the first sealing valve 5 is opened and the used disinfectant solution is discharged through the discharge port 4. It should be noted that the storage chamber 202 is also provided with an observation port (not shown in the figure) for observing the remaining amount of disinfectant solution and a filling port for replenishing disinfectant solution on one side.

[0030] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A plant callus cleaning and sterilization device, comprising a base (1) and a processing box (2) disposed on the base (1), characterized in that, The processing box (2) is provided with a reciprocating loading plate (10), and multiple cell units (11) for placing plant callus tissue are arranged on the loading plate (10). Each cell unit (11) has several through holes (12) at the bottom. The cleaning and sterilization device also includes a spray assembly (3) for rinsing the plant callus tissue after soaking and sterilization with sterile water.

2. The plant callus cleaning and sterilization device according to claim 1, characterized in that, Each of the cells (11) has a soft silicone layer on its inner wall.

3. The plant callus cleaning and sterilization device according to claim 1, characterized in that, The inner walls of the processing box (2) are provided with horizontally oriented sliding grooves (13) on both sides. The two sides of the loading plate (10) are respectively locked in the corresponding sliding grooves (13). The loading plate (10) is provided with a first boss (14) and a second boss (16). A number of elastic reset members (15) are provided between the first boss (14) and the inner wall of the processing box (2). The processing box (2) is provided with a rotatable flower-shaped member (17), and the side of the flower-shaped member (17) abuts against the second boss (16). The processing box (2) is also provided with a driving member for driving the flower-shaped member (17) to rotate.

4. The plant callus cleaning and sterilization device according to claim 1, characterized in that, The upper side of the treatment box (2) is open, and the spray assembly (3) includes a detachable spray plate (301) located at the opening of the treatment box (2) and a delivery pipe (303) for delivering sterile water to the spray plate (301). Spray holes (302) are evenly distributed at the bottom of the spray plate (301).

5. The plant callus cleaning and sterilization device according to claim 1, characterized in that, The processing box (2) is divided into an immersion chamber (201) and a storage chamber (202) by a partition. The loading plate (10) is located in the immersion chamber (201). The immersion chamber (201) has a discharge port (4) on one side and a first sealing valve (5) at the discharge port (4). The partition has an injection port (6) and a second sealing valve (7) at the injection port (6). The storage chamber (202) has a sealing piston (8). The bottom of the storage chamber (202) has a telescopic component (9) and the telescopic end of the telescopic component (9) is connected to the sealing piston (8).

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