Artemia hatching device
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-19
- Publication Date
- 2026-08-11
AI Technical Summary
[0003]丰年虫卵孵化过程中,对溶氧要求非常高,局部含氧量不足,或是孵化过程不能保持丰年虫卵悬浮,出现卵沉底堆积和相互挤压,会形成厌氧环境,导致卵缺氧而死亡,死亡会释放毒素,感染周围的卵,导致“连锁腐败”,孵化率极低
[0043](1)提供了一种全新的丰年虫卵孵化罐,内设有立体充氧装置,能够在孵化罐内形成完美的闭环循环水流,使丰年虫卵粒随水流柔和地循环悬浮和规则运动,减少硬性碰撞;
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Figure CN224611592U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of aquaculture feed technology, and more specifically, to an Artemia incubation device. Background Technology
[0002] Artemia, also known as saltwater brine shrimp, is a palatable food source for fish and shrimp fry and ornamental fish. It plays an important role in providing nutrition for fish and shrimp fry and ensuring their growth. Artemia is also widely used in ichthyology, fish ecology field practice, laboratory culture of fish fry, and fish fry breeding.
[0003] During the hatching process of Artemia eggs, the dissolved oxygen requirement is very high. If the local oxygen content is insufficient, or if the Artemia eggs cannot be kept suspended during the hatching process, the eggs will sink to the bottom and pile up and squeeze each other, which will create an anaerobic environment, causing the eggs to die from hypoxia. The dead eggs will release toxins, which will infect the surrounding eggs, leading to a "chain reaction of decay" and an extremely low hatching rate.
[0004] To keep Artemia eggs suspended and prevent them from sinking, existing hatching equipment typically uses continuous and vigorous aeration to tumble the eggs and prevent them from settling. This process can easily cause violent collisions between eggs or between eggs and the hatching equipment. For eggs of poor quality, with thin shells or minor cracks, these violent collisions can directly cause the shells to rupture, killing the embryo inside. Furthermore, continuous mechanical impact can wear down the protective layer on the eggshell surface, making it more susceptible to bacterial or fungal infection. This is the main reason for the low hatching rate of existing Artemia egg hatching equipment. At the same time, the operation of existing Artemia egg hatching devices is difficult to control, resulting in large batch-to-batch variations, leading to low hatching rates and poor egg quality that cannot meet the high standards required for export.
[0005] Therefore, there is an urgent need to find a new type of hatching device that can ensure that all Artemia eggs can be stably suspended or move regularly, while avoiding hard collisions caused by violent inflation, thereby improving the hatching rate of Artemia eggs. Utility Model Content
[0006] To address the aforementioned problems, this invention provides an Artemia hatching device. By incorporating a three-dimensional oxygenation system into the hatching tank, a continuous oxygen supply is ensured during the Artemia hatching process. This system comprises three oxygenation components: the first layer is oxygenated via a first oxygenation pipe, the second layer via a ring-shaped pipe, and the third layer via a bottom oxygenation pipe. This creates a perfect circulating water flow within the hatching tank, allowing the eggs to circulate gently and move regularly with the water flow, reducing hard collisions. Simultaneously, the addition of sodium hyaluronate and sodium citrate to the incubation agent maintains the suspension effect of the eggs during hatching and provides a better microenvironment for hatching. Combined with the three-dimensional oxygenation system, this effectively improves the hatching rate of Artemia eggs, demonstrating broad application prospects.
[0007] On the one hand, this utility model provides a hatching device for Artemia worms, the hatching device including a hatching tank with openings at the top and bottom, the hatching tank being equipped with a three-dimensional oxygenation device, the three-dimensional oxygenation device being equipped with an annular oxygenation component, the annular oxygenation component driving the formation of an annular water flow inside the hatching tank.
[0008] In order to keep the brine shrimp eggs suspended and prevent them from clumping during the hatching process, they need to be continuously and violently aerated. This process will generate a lot of turbulence, eddies and bubbles in the water. The eggs are thrown and rolled at high speed by the irregular water flow, which can easily cause violent physical collisions that can damage or kill the eggs.
[0009] This invention features a three-dimensional oxygenation device installed in the hatchery. Through the continuous ring-shaped oxygenation component, the water flow maintains a regular and continuous ring-shaped circulation, eliminating the need for vigorous aeration. The eggs can also circulate and rotate gently with the water flow, effectively reducing hard collisions with the container walls and minimizing impact.
[0010] The aforementioned annular oxygenation component refers to a certain number of oxygenation heads evenly arranged around the incubation tank. The oxygenation direction of these oxygenation heads is along the inner wall of the incubation tank. Therefore, when these oxygenation heads are turned on simultaneously, an annular oxygenation method can be formed. In other words, the oxygen injected into the incubation tank can maintain a certain annular flow direction, which can continue to drive the water flow (such as incubator) to circulate in an annular manner in the incubation tank. At the same time, it can cause the eggs in the water flow to be suspended and maintain a regular annular movement, so that they will not fall into irregular tumbling and effectively prevent the impact and damage caused by hard collisions.
[0011] Furthermore, the three-dimensional oxygenation device includes two layers of annular oxygenation components, each layer including three evenly arranged oxygenation heads; the first layer is located above the second layer; the positions of the oxygenation heads of the first and second layers are staggered.
[0012] The three-dimensional oxygenation device provided by this utility model is equipped with two layers of annular oxygenation components. The oxygenation direction of the two annular oxygenation components is the same, such as both rotating counterclockwise or both rotating clockwise. The combination of the two layers can form a spiral-like two-layer annular water flow.
[0013] By continuously oxygenating the upper annular oxygenation component, the Artemia eggs located in the upper water flow can quickly enter the annular flow channel and smoothly transition to the second-layer annular circulating water flow, starting regular annular movement, thereby solving the oxygen supply problem in the upper layer.
[0014] In some designs, the positions of the first and second layers of oxygenation heads are staggered. This means that the positions of the three oxygenation heads in the first layer are not the same as those in the second layer, but are staggered along a circular track. In other words, there are no oxygenation heads directly below the first layer and no oxygenation heads directly above the second layer. The second layer is equivalent to rotating the three evenly arranged oxygenation heads in the first layer by 30 degrees clockwise or counterclockwise along the circumference. This helps the first layer of oxygenation heads to push the upper layer of Artemia eggs more smoothly into the circular circulating water flow of the second layer.
[0015] Furthermore, the three oxygenation heads of each layer are connected by connecting pipes to form a ring pipe, including a first ring pipe and a second ring pipe, and each layer is supplied with oxygen from a unified source; the incubation tank includes two parts: a cylindrical tank and a conical tank, with the cylindrical tank located above the conical tank; the second ring pipe is located in the lower middle position of the cylindrical tank.
[0016] Furthermore, the three oxygen filling heads in each layer are connected by connecting pipes to form a ring pipe. The three oxygen filling heads in the first layer are connected by connecting pipes to form a first ring pipe, and the three oxygen filling heads in the second layer are connected by connecting pipes to form a second ring pipe.
[0017] Furthermore, both the first and second ring pipes are supplied with oxygen from a unified source.
[0018] Furthermore, the incubation tank comprises two parts: a cylindrical tank and a conical tank, with the cylindrical tank located above the conical tank.
[0019] Furthermore, the second annular pipe is located in the lower middle position of the cylindrical tank.
[0020] Three evenly arranged oxygenation heads, supplied from a unified source, continuously provide oxygen from three directions around the circumference, combining to form a ring-shaped airflow. This airflow propels the hatching tank to form a ring-shaped water flow, causing the Artemia eggs to suspend and maintain a regular ring-shaped movement.
[0021] The shape of the hatchery and the position of the annular pipe also significantly influence the formation of the annular water flow. The hatchery is divided into two parts: an upper cylindrical part and a lower conical part. The annular pipe is positioned in the lower middle of the cylindrical part. This structural design allows the water to first form an annular flow at the cylindrical part, then spiral downwards along the conical part, causing the Artemia eggs to move in a regular annular cycle. The eggs circulate along the smooth inner wall of the conical shell and gradually enter the lower water flow. This process avoids collisions with the container walls and is highly orderly.
[0022] Furthermore, the three-dimensional oxygenation device also includes a bottom oxygenation pipe in the third layer, which is connected to the bottom outlet pipe of the incubator and is used to fill oxygen from the bottom upwards.
[0023] The bottom oxygenation pipe inflates air from the bottom upwards, causing the eggs that circle the conical inner wall and gradually approach the center of the bottom to be pushed upwards into the upper water flow by the oxygen provided by the bottom oxygenation pipe. Since the bottom is located at the center of the entire cylindrical tank, the upward-moving eggs are also located at the center, preventing them from colliding with the eggs in the circular motion. The eggs entering the upper layer will then enter the second layer of circular circulating water flow under the action of the first oxygenation pipe, starting a regular circular motion. The entire motion process forms a complete and continuous closed loop, with all eggs queuing up in sequence for cyclical motion without colliding with each other.
[0024] Furthermore, the bottom of the incubator is provided with a funnel valve, and the funnel valve has an air filling channel in the middle; during the incubation process, the funnel valve is closed, and oxygen is filled into the incubator through the air filling channel.
[0025] In some embodiments, the funnel valve is shaped like a downward-facing funnel, made of rubber, and has a certain degree of elasticity, which can better seal the bottom outlet of the incubator.
[0026] In some embodiments, the hatchery has a discharge port at the bottom, through which hatched Artemia larvae can be collected. During hatching, the bottom is blocked by a funnel valve to prevent leakage of Artemia eggs, and oxygen is supplied to the hatchery through an air inlet in the middle of the funnel valve via a bottom oxygen supply pipe.
[0027] Furthermore, it also includes a lighting lamp that provides illumination to the interior of the incubator from an opening at the top of the incubator.
[0028] Furthermore, it also includes a support frame for supporting the incubator and lighting, and the support frame has a grid pattern for supporting the bottom of the incubator.
[0029] Artemia eggs typically take 18 to 36 hours to hatch from release, requiring at least 3 hours of light. Therefore, lighting should be installed above the hatching tank to provide the necessary light conditions for hatching.
[0030] The support also has a knob for adjusting the height of the light source from the hatching tank, thereby regulating the appropriate lighting environment for the hatching of Artemia eggs.
[0031] Furthermore, this utility model provides a method for hatching Artemia eggs, wherein the method employs the hatching device described above and includes the following steps:
[0032] (1) Prepare an incubator containing sodium hyaluronate, sodium citrate and sea salt water, and place it in an incubator tank;
[0033] (2) Soak the brine shrimp eggs in sea salt water until they are saturated with water, and then incubate them in an incubation agent.
[0034] Extensive research has demonstrated that adding appropriate amounts of sodium hyaluronate and / or sodium citrate to the incubator helps improve the hatching rate of Artemia salina eggs. This is likely because sodium hyaluronate and / or sodium citrate can maintain a certain degree of suspension in the Artemia salina eggs, reducing the risk of aggregation or settling.
[0035] The exoskeleton (chitin) of Artemia eggs has a certain degree of stickiness, especially after absorbing water. When the eggs come into contact with each other, this stickiness easily causes them to clump together. Once clumping occurs during hatching, it is difficult to disperse them again by shaking or aeration. Adding an appropriate amount of sodium hyaluronate to the incubator helps to increase the steric hindrance on the egg surface, preventing clumping and thus increasing the hatching rate of Artemia. Simultaneously, sodium hyaluronate can also increase the viscosity of the incubator to some extent, slowing down the deposition rate of Artemia eggs and making them more difficult to settle, thus having a certain suspension-promoting effect. In addition, sodium citrate can be used to adjust the pH of the incubator, also slowing down the deposition of Artemia eggs in the incubator, thus also contributing to the suspension of Artemia eggs and preventing clumping.
[0036] In some embodiments, the mass ratio of sodium hyaluronate to sodium citrate is 1:1.
[0037] Furthermore, in the incubation device, the oxygenation rate of the first layer is 1-3 L / min; the oxygenation rate of the second layer is 1-3 L / min; and the oxygenation rate of the bottom oxygenation pipe is 1-3 L / min.
[0038] A suitable amount of oxygen can ensure the suspension and oxygen supply needs of Artemia eggs during the hatching process, while preventing damage or loss due to excessive aeration. Therefore, for the three-layer three-dimensional oxygenation device provided by this utility model, it is necessary to select a suitable amount of oxygen to improve the hatching rate of Artemia eggs.
[0039] Furthermore, the incubation conditions for the incubation in step (2) are: temperature 28-30℃, light intensity 1000Lux-3000Lux.
[0040] In some methods, step (3) is also included: after hatching, the larvae are left to stand, layered, and the outlet pipe at the bottom of the hatching tank is opened to collect the brine shrimp larvae located in the lower layer.
[0041] On another aspect, this invention provides the use of a composition for preparing a reagent that enhances the hatching effect of Artemia salina eggs, wherein the composition is sodium hyaluronate and sodium citrate.
[0042] The beneficial effects of this utility model are as follows:
[0043] (1) A brand-new Artemia egg hatchery is provided, which is equipped with a three-dimensional oxygenation device that can form a perfect closed-loop circulating water flow in the hatchery, so that Artemia eggs can be gently circulated and suspended and move regularly with the water flow, reducing hard collisions.
[0044] (2) By adding sodium hyaluronate and sodium citrate to the incubator, the suspension effect of the eggs during the hatching process can be maintained, and a better hatching microenvironment can be provided. Combined with the three-dimensional oxygenation device, the hatching rate of Artemia eggs can be effectively improved. Attached Figure Description
[0045] Figure 1 A schematic diagram of the overall structure of the brine shrimp hatching device;
[0046] Figure 2 This is a schematic diagram of the overall structure of the incubator;
[0047] Figure 3 This is a top view of the incubation tank;
[0048] Figure 4 This is a cross-sectional view of the incubator.
[0049] Figure 5 This is a schematic diagram of the funnel valve.
[0050] Figure 6 This is a schematic diagram of the overall structure of the support. Detailed Implementation
[0051] The preferred embodiments of the present invention will be described in further detail below with reference to the accompanying drawings. It should be noted that the embodiments described below are intended to facilitate the understanding of the present invention and do not limit it in any way. All features disclosed in the embodiments of the present invention, or all steps in all methods or processes disclosed, can be combined in any way except for mutually exclusive features and / or steps.
[0052] Example 1: Artemia incubation device provided by this utility model
[0053] The brine shrimp hatching device 1 provided in this embodiment is as follows: Figures 1-6 As shown. Among them, as Figures 1-4As shown, the incubation device 1 includes an incubation tank 2 with openings at the top and bottom. The incubation tank 2 is equipped with a three-dimensional oxygenation device 3, which has an annular pipe oxygenation component 4. The annular oxygenation component 4 promotes the formation of annular water flow inside the incubation tank 2. The annular oxygenation component 4 refers to a certain number of oxygenation heads 6 evenly arranged around the incubation tank 2. The oxygenation direction of these oxygenation heads 6 is along the horizontal direction of the inner wall 11 of the incubation tank. Therefore, when these oxygenation heads 6 are turned on at the same time, an annular oxygenation mode can be formed. That is to say, the oxygen sprayed into the incubation tank 2 can maintain a certain annular flow direction, and can continue to drive the water flow (such as incubation agent) to circulate in an annular manner in the incubation tank 2. At the same time, it can suspend the eggs in the water flow and maintain a regular annular movement, so as not to fall into irregular rolling, effectively preventing the impact and damage caused by hard collisions.
[0054] The three-dimensional oxygenation device 3 includes two layers of annular oxygenation components 4, each layer comprising three evenly arranged oxygenation heads 6; the first layer 5 is located above the second layer 7; the positions of the oxygenation heads 6 in the first layer 5 and the second layer 7 are staggered. The three-dimensional oxygenation device 3 features two layers of annular oxygenation components 4, with both layers rotating in the same direction, such as counter-clockwise or clockwise. The combination of the two layers forms a spiral-like two-layer annular water flow. Continuous oxygenation through the upper annular oxygenation component 4 helps the Artemia eggs in the upper water flow to quickly enter the annular channel and smoothly transition to the annular circulating water flow of the second layer 7, initiating regular annular movement, thus solving the oxygenation problem in the upper layer. The positions of the oxygenation heads 6 in the first layer 5 and the second layer 7 are staggered. This means that the positions of the three first-layer oxygenation heads 8 and the three second-layer oxygenation heads 9 are not the same, but are designed to be staggered along a circular track. In other words, there are no second-layer oxygenation heads 9 directly below the first-layer oxygenation heads 8, and there are no first-layer oxygenation heads 8 directly above the second-layer oxygenation heads 9. The second layer 7 is equivalent to rotating the circle formed by the evenly arranged first-layer oxygenation heads 8 in the first layer 6 by 30 degrees clockwise or counterclockwise. This setting helps the first-layer oxygenation heads 8 to push the upper layer of Artemia eggs more smoothly into the circular circulating water flow of the second layer 7.
[0055] like Figure 3 and Figure 4As shown, the three oxygenation heads 6 in each layer are connected by connecting pipes 10 to form a ring pipe, including the first ring pipe 11 of the first layer 5 and the second ring pipe 12 of the second layer 7, which are supplied with oxygen from a unified source. The first layer 5 is supplied with oxygen by the first oxygen pipe 31, and the second layer 7 is supplied with oxygen by the second oxygen pipe 32. The hatching tank 2 includes two parts: a cylindrical tank 13 and a conical tank 14. The cylindrical tank 13 is located above the conical tank 14. The second ring pipe 12 is located in the lower middle position of the cylindrical tank 13. The three evenly arranged oxygenation heads 6 in each layer are supplied with oxygen from a unified source, continuously providing oxygen from three directions around the circumference. The combination forms a ring airflow, which drives the formation of a ring water flow inside the hatching tank 2, causing the Artemia eggs to suspend and maintain a regular ring movement. Furthermore, as can be seen from the diagram, each oxygenation head 6, after extending into the incubator 2, is not in a straight line, but rather makes a 90-degree turn 30 close to the inner wall 11 of the incubator. Therefore, the oxygenation process also proceeds along the inner wall 11, pushing the water flow to form a circular circulating flow. The shape of the incubator 2 and the position of the second annular pipe 12 also significantly influence the formation of the circular water flow. The incubator 2 is divided into upper and lower parts: the upper part is cylindrical, and the lower part is conical. The second annular pipe 12 is positioned at the lower middle position 15 of the cylindrical tank body 13. In this embodiment, it is preferable that the second annular pipe 12 is slightly higher than the interface 16 between the cylindrical tank body 13 and the conical tank body 14, with a straight-line distance of 2 cm from the interface 16. This structural design allows the water flow to first form a circular flow at the position of the cylindrical tank 13, and then spiral downwards along the conical tank 14, causing the Artemia eggs to move in a regular circular cycle, circling around the smooth conical inner wall 17 and gradually entering the lower water flow. This process does not collide with the container wall and is orderly.
[0056] like Figures 1-4 As shown, the three-dimensional oxygenation device 3 also includes a bottom oxygenation pipe 19 in the third layer 18. The bottom oxygenation pipe 19 is connected to the bottom outlet pipe 20 of the hatching tank 2 and is used to supply oxygen from the bottom 21 upwards. The bottom oxygenation pipe 19 supplies oxygen from the bottom 21 upwards, which allows the eggs that are surrounded by the conical inner wall 17 and gradually approach the center of the bottom 21 to be pushed upwards from the bottom 21 to the upper water flow under the action of the oxygen provided by the bottom oxygenation pipe 19. Moreover, since the bottom 21 is located at the center position 22 of the entire cylindrical tank 13, the upward-moving eggs are also located at the center position 22 and will not collide with the eggs in the circular motion. The eggs that enter the upper layer will then enter the circular circulating water flow of the second layer 7 under the action of the first layer oxygenation head 8 and begin regular circular motion. The entire motion process forms a complete and continuous closed loop, and all the eggs line up in sequence for cyclical motion without colliding with each other.
[0057] like Figures 3-5As shown, the bottom 21 of the hatching tank 2 is equipped with a funnel valve 23, and an air inlet 24 is located in the middle of the funnel valve 23. During incubation, the funnel valve 23 is closed, and oxygen is supplied to the hatching tank 2 through the air inlet 24. The funnel valve 23 is shaped like a downward-facing funnel and is made of rubber, which has a certain degree of elasticity and can better seal the outlet of the bottom 21 of the hatching tank 2. The outlet of the bottom 21 of the hatching tank 2 is also the discharge port 25, and the hatched Artemia larvae can be collected through the bottom outlet pipe 20. During incubation, the bottom 21 is blocked by the funnel valve 23 to prevent the leakage of Artemia eggs, and oxygen is supplied to the hatching tank 2 through the air inlet 24 in the middle of the funnel valve 23 via the bottom oxygen supply pipe 19.
[0058] like Figure 6 As shown, the incubation device 1 also includes a lighting lamp, which provides illumination to the interior of the incubation tank 2 through the opening 26 at the top. The lighting lamp above the incubation tank 2 is provided to provide the necessary light conditions for the hatching of Artemia eggs. A support frame 27 is also provided, which supports the incubation tank 2 and the lighting lamp. The upper end 25 of the support frame 27 is used to hold the lighting lamp and has an adjustment knob 33 for adjusting the height of the lighting lamp. The support frame 27 also has a grid-like opening 28 for supporting the bottom 21 of the incubation tank 2. In this embodiment, the incubation tank 2 has a volume of 12L, and the support frame has two layers with a total height of 56cm. The upper layer is 28cm high, the lower layer is 28cm high, and the width is 28cm.
[0059] Example 2: Hatching method of Artemia
[0060] This embodiment uses the incubation device 1 provided in Embodiment 1 for the incubation of Artemia salina eggs. The specific steps are as follows:
[0061] (1) Prepare an incubator containing sodium hyaluronate, sodium citrate and sea salt water, and place it in an incubator tank;
[0062] Sodium hyaluronate, sodium citrate, and sea salt were added to pure water to prepare a total of 8L of incubator containing 2mg / L sodium hyaluronate, 2mg / L sodium citrate, and 25g / L sea salt. The incubator was placed in an incubator and sterilized under ultraviolet light for 30 minutes.
[0063] (2) Soak the Artemia eggs in sea salt water until they are saturated with water, and then incubate them with an incubation agent.
[0064] Take 24g of Artemia elegans eggs, soak them in 25g / L sea salt water for 3 hours until they are saturated, and then quickly transfer them to an incubation tank for hatching. The incubation conditions are: temperature 28℃, light intensity 2000Lux, light duration 4 hours, oxygen supply of the first layer in the incubation tank at 2L / min; oxygen supply of the second layer at 2L / min; and oxygen supply of the bottom oxygen pipe at 3L / min. The incubation time is 30 hours.
[0065] (3) Let it stand still to separate and collect the Artemia larvae;
[0066] After hatching is complete, stop oxygenation and let it stand for 10 minutes. The eggshells will float to the top, and the brine shrimp larvae will settle in the bottom layer. Open the valve of the outlet pipe at the bottom of the hatching tank and collect the brine shrimp larvae in the bottom layer.
[0067] Example 3: Effects of different incubation agents on the hatching effect of Artemia salina
[0068] In this embodiment, Artemia eggs were hatched according to the method provided in Example 2. The hatching agents were prepared using different formulations as shown in Table 1. The effects of different hatching agents on the hatching rate and survival rate of Artemia eggs were investigated. The hatching rate was detected by using a hemocytometer to detect the number of hatched Artemia nauplii larvae. Hatching rate = (number of hatched nauplii larvae / initial number of eggs) * 100%. The survival rate was detected by the phototaxis test. If most of the larvae gathered in the bright area, it indicated a high overall survival rate. If the number of larvae in the bright area and the dark area were similar, it indicated a low survival rate. The test results are shown in Table 1.
[0069] Table 1. Effects of different incubation agents on the hatching efficiency of Artemia worms
[0070]
[0071]
[0072] As shown in Table 1, using different incubators to hatch Artemia eggs has a significant impact on their hatching rate and survival rate.
[0073] The addition of sodium hyaluronate, compared with the blank control (seawater), effectively improved the hatching rate and survival rate of Artemia salsa eggs. This may be because the addition of sodium hyaluronate helps to increase the steric hindrance on the surface of the eggs, preventing aggregation and thus increasing the hatching rate of Artemia salsa. At the same time, sodium hyaluronate can also increase the viscosity of the incubator to a certain extent, making it slower and more difficult for Artemia salsa eggs to settle, thus producing a certain suspension-promoting effect on Artemia salsa eggs. In addition, sodium hyaluronate also has a certain nutrient-promoting effect.
[0074] Adding sodium citrate alone can improve the hatching rate to some extent, possibly because sodium citrate can adjust the pH of the incubator and also slows down the deposition of Artemia eggs in the incubator. However, the effect of adding sodium citrate alone is relatively limited.
[0075] The addition of sodium hyaluronate and sodium citrate at the same time has a significant synergistic effect, and the hatching rate and survival rate of Artemia eggs are significantly improved. The effect is significantly better than that of the hatching agents in other groups. Therefore, the hatching agent in group 5 is the optimal hatching agent formula.
[0076] Although the present invention has been disclosed above, it is not limited thereto. Its scope can be expanded according to its applications in agriculture and forestry. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention; therefore, the scope of protection of the present invention should be determined by the scope defined in the claims.
Claims
1. A brine shrimp hatching device, characterized in that, The incubation tank includes an opening at the top and bottom. The incubation tank is equipped with a three-dimensional oxygenation device, which has an annular oxygenation component. The annular oxygenation component promotes the formation of an annular water flow inside the incubation tank.
2. The incubation apparatus as described in claim 1, characterized in that, The three-dimensional oxygenation device includes two layers of annular oxygenation components, each layer including three evenly arranged oxygenation heads; the first layer is located above the second layer; the positions of the oxygenation heads in the first and second layers are staggered.
3. The incubation apparatus as described in claim 2, characterized in that, The three oxygen supply heads in each layer are connected by connecting pipes to form a ring pipe. The three oxygen supply heads in the first layer are connected by connecting pipes to form the first ring pipe, and the three oxygen supply heads in the second layer are connected by connecting pipes to form the second ring pipe.
4. The incubation apparatus as described in claim 3, characterized in that, Both the first and second ring pipelines are supplied with oxygen from a unified source.
5. The incubation apparatus as described in claim 4, characterized in that, The incubation tank consists of two parts: a cylindrical tank and a conical tank, with the cylindrical tank located above the conical tank.
6. The incubation apparatus as described in claim 5, characterized in that, The second annular pipe is located in the lower middle part of the cylindrical tank.
7. The incubation apparatus as described in claim 6, characterized in that, The three-dimensional oxygenation device also includes a bottom oxygenation pipe in the third layer, which is connected to the outlet pipe at the bottom of the incubator and is used to fill oxygen from the bottom up.
8. The incubation apparatus as described in claim 7, characterized in that, The bottom of the incubator is equipped with a funnel valve, and the funnel valve has an air filling channel in the middle. During the incubation process, the funnel valve is closed, and oxygen is filled into the incubator through the air filling channel.
9. The incubation apparatus as described in claim 8, characterized in that, It also includes a lighting fixture that provides illumination to the interior of the incubator from an opening at the top.
10. The incubation apparatus as described in claim 9, characterized in that, It also includes a support frame for supporting the incubator and the lighting lamp. The support frame has a grid pattern for supporting the bottom of the incubator and an adjustment knob for adjusting the height of the lighting lamp.