A concentrated filtration and storage device

Abstract

This utility model relates to the field of liquid storage and filtration technology, specifically a concentrated filtration and storage device. The utility model includes a syringe, a connector, a filter box, and a storage chamber. The connector includes a Y-shaped tee. A one-way valve is installed on one side of the Y-shaped tee, and a two-way valve is installed on the other side. The other end of the one-way valve is fixedly connected to the filter box via a pipe. A waste liquid chamber is located below the filter box. A top cover is installed at the top of the storage chamber. A sample addition tube, an inflow tube, and an outlet tube are fixedly inserted into the outer wall of the top cover. The filter box is connected to the inflow tube via a pipe. A manual squeeze valve is installed outside the pipe. The outlet tube is connected to the other end of the two-way valve via a pipe. This utility model adopts a minimalist design, is easy to assemble and disassemble, and has zero equipment dependence. Compared with existing methods, it greatly reduces costs, simplifies operation, significantly shortens processing time, and improves the efficiency of concentrated filtration and storage of liquids.

Description

Technical Field

[0001] This utility model relates to the field of liquid storage and filtration technology, specifically a concentrated filtration and storage device. Background Technology

[0002] The concentration, filtration, and storage device is a composite device that combines concentration, filtration, and storage functions. It is mainly used for the pretreatment and concentration of liquids. The concentration, filtration, and storage device is a highly integrated multifunctional device. It is mainly used in various scenarios that require pretreatment of fluid samples, concentration of target substances, removal of impurities, and subsequent stabilization and storage of liquids. It is widely used in fields such as biopharmaceuticals, molecular biology, environmental monitoring, food inspection, and chemical synthesis.

[0003] In existing technologies, when concentrating and removing impurities from nanoscale particles in trace liquid samples, equipment such as ultracentrifuges, tangential flow systems, and ion exchange chromatography systems are often used. These large-scale equipment are cumbersome to use, complex to operate, costly, time-consuming, and have low target recovery rates, resulting in significant deficiencies in the efficiency and convenience of concentrating, filtering, and storing liquid samples. Utility Model Content

[0004] The purpose of this invention is to provide a concentrated filtration and storage device to solve the problems mentioned in the background art.

[0005] The objective of this utility model can be achieved through the following technical solutions:

[0006] A concentrated filtration and storage device includes a syringe, a connector, a filter box, and a storage chamber. The connector includes a Y-shaped tee. A one-way valve is installed on one side of the Y-shaped tee, and a two-way valve is installed on the other side of the Y-shaped tee. The other end of the one-way valve is fixedly connected to the filter box through a pipe. A waste liquid chamber is provided below the filter box. A top cover is provided at the upper end of the storage chamber. A sample addition tube, an inflow tube, and an outflow tube are fixedly inserted into the outer wall of the top cover.

[0007] The filter box is connected to the inflow pipe through a pipe, the suction pipe is connected to the other end of the one-way valve through a pipe, and the sample addition tube is equipped with a filter.

[0008] A manual squeeze valve is installed outside the pipe between the filter box and the inflow pipe. The bottom ends of the sample addition tube, the inflow pipe and the suction tube are all located inside the liquid storage chamber.

[0009] Preferably, the filter box has a cover at both the front and rear ends, and a snap-fit ​​assembly is provided between the outer wall of the cover and the outer wall of the filter box. The snap-fit ​​assembly includes a buckle and a plate. The buckle is rotatably connected to the outer wall of the cover via a shaft, and the plate is fixedly installed on the outer wall of the filter box. The buckle and the plate cooperate with each other. Luer connectors are fixedly connected to the outer walls of both covers, and filter outlet tubes are fixedly connected to both Luer connectors.

[0010] Preferably, the filter box is provided with a second interlayer, an ultrafiltration membrane and a silicone sealing ring at both the front and rear ends. The filter box is divided into internal flow channels by the two second interlayers, ultrafiltration membranes and silicone sealing rings. The two box covers are fixedly installed with a first interlayer. The box covers are divided into filter chambers by the first interlayer.

[0011] Preferably, both ends of the filter box are fixedly connected to Luer connectors II, and both Luer connectors II are connected to the internal flow channel.

[0012] Preferably, the upper end of the waste liquid chamber is fixedly connected to a vent pipe and two inflow pipes, and the upper ends of the two inflow pipes are respectively connected to two filter pipes through pipes. A filter is fixedly installed on the upper end of the vent pipe, and the bottom ends of the vent pipe and the two inflow pipes are all located inside the waste liquid chamber.

[0013] The beneficial effects of this utility model are:

[0014] This invention features a minimalist design, easy assembly and disassembly, and zero equipment dependence. Compared to existing methods, it significantly reduces costs, simplifies operation, and drastically shortens processing time. All components can be pre-sterilized and used only once. In a sterile environment, aseptic operation can be achieved throughout the entire experimental process, avoiding sample contamination and not affecting downstream product analysis and use. Furthermore, different specifications of components can be equipped or customized according to sample volume, such as injections with different ranges, tubing with different diameters, ultrafiltration membranes with different areas and matching filter cartridges, and storage chambers with different volumes. It has a very small dead space volume, ensuring a high recovery rate for precious samples. The entire flow path is visualized, and the flow rate can be adjusted in a timely manner with manual control, minimizing structural damage to the sample. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a cross-sectional view of the liquid storage chamber and filter box under the overall structure of this utility model;

[0018] Figure 3 This is a cross-sectional view of the filter box in this utility model;

[0019] Figure 4 This is a schematic diagram of the internal structure of the filter box in this utility model;

[0020] Figure 5 This is a cross-sectional view of the liquid storage chamber in this utility model;

[0021] Figure 6 This is a cross-sectional view of the waste liquid chamber in this utility model;

[0022] Figure 7 This is a structural schematic diagram of the filter box, box cover and snap-fit ​​assembly in this utility model.

[0023] The attached figures are labeled as follows:

[0024] 1. Syringe; 2. Y-type three-way valve; 3. One-way valve 1; 4. Top cover; 5. Filter box; 6. Waste liquid chamber; 7. Manual squeeze valve; 8. Liquid storage chamber; 9. Filter 1; 10. Sample dispensing tube; 11. Inflow tube 1; 12. Silicone sealing ring; 13. Buckle; 14. Filter outlet tube; 15. Luer connector 1; 16. Filter outlet chamber; 17. Luer connector 2; 18. Internal flow channel; 19. Jacket 1; 20. Ultrafiltration membrane; 21. Suction tube; 22. One-way valve 2; 23. Inflow tube 2; 24. Vent tube; 25. Filter 2; 26. Box cover; 27. Jacket 2; 28. Clamping plate. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0026] A concentrated filtration and storage device, such as Figures 1-7As shown, the device includes a syringe 1, a connector, a filter box 5, and a storage chamber 8. The connector includes a Y-type tee 2. A one-way valve 3 is installed on one side of the Y-type tee 2, and a one-way valve 22 is installed on the other side of the Y-type tee 2. The other end of the one-way valve 3 is fixedly connected to the filter box 5 through a pipe. A waste liquid chamber 6 is installed below the filter box 5. A top cover 4 is installed at the top of the storage chamber 8. A sample addition tube 10, an inflow tube 11, and an aspiration tube 21 are fixedly inserted into the outer wall of the top cover 4. The filter box 5 is connected to the inflow tube 11 through a pipe. The aspiration tube 21 is connected to the other end of the one-way valve 22 through a pipe. A filter 9 is installed at the top of the sample addition tube 10.

[0027] Both check valve 1 (3) and check valve 2 (22) are model S10A12B, which can ensure the directional flow of liquid and air and prevent liquid and air backflow.

[0028] A manual squeeze valve 7 is installed outside the pipe between the filter box 5 and the inflow pipe 11. The bottom ends of the sample tube 10, the inflow pipe 11 and the suction tube 21 are all located inside the liquid storage chamber 8.

[0029] The manual squeeze valve 7, model GJ841X, is fitted outside the pipe. The manual squeeze valve 7 is a key component of this device. Its primary function is to precisely control the transmembrane pressure. By rotating the valve knob, the operator can precisely squeeze and adjust the inner diameter of the pipe flowing from the filter box 5 to the liquid storage chamber 8, thereby artificially increasing the pipe resistance. When the syringe 1 injects the sample at a constant speed, the increased resistance at the pipe outlet will cause the pressure in the internal flow channel 18 of the filter box 5 to rise. The pressure difference generated at this time, that is, the pressure difference between the internal flow channel 18 and the filtration chamber 16, is the transmembrane pressure. It is the fundamental driving force for the solvent and small molecules in the sample to pass through the ultrafiltration membrane 20.

[0030] Secondly, the manual squeeze valve 7 can prevent membrane damage and optimize filtration efficiency. By manually adjusting it, excessive transmembrane pressure caused by excessive injection speed of syringe 1 can be avoided, thereby protecting the ultrafiltration membrane 20 from mechanical damage, such as being crushed or compacted. At the same time, the operator can find an optimal degree of squeeze to achieve the most efficient filtration flux while ensuring membrane safety.

[0031] Both ends of the filter box 5 are provided with a cover 26. A snap-fit ​​assembly is provided between the outer wall of the cover 26 and the outer wall of the filter box 5. The snap-fit ​​assembly includes a buckle 13 and a locking plate 28. The buckle 13 is rotatably connected to the outer wall of the cover 26 via a shaft. The locking plate 28 is fixedly installed on the outer wall of the filter box 5, and the buckle 13 and the locking plate 28 cooperate with each other. Luer connectors 15 are fixedly connected to the outer walls of both covers 26, and both Luer connectors 15 are fixedly connected to the filter outlet tube 14. The buckle 13 in the snap-fit ​​assembly, in conjunction with the locking plate 28, is used to... The cover 26 is fixed to the filter box 5. The buckle 13 is rotatably connected to the cover 26 via a shaft and is not separated from the cover 26. By rotating the buckle 13, it is tightly locked onto the outside of the plate 28, thus fixing the cover 26 onto the filter box 5. The cover 26 can be easily disassembled and assembled by opening and closing the buckle 13. The buckle 13 and the plate 28 fit together and are both made of plastic, which has a certain deformation force. Under the action of external force and with the help of friction, they can be tightly locked. This is existing technology and will not be described in detail.

[0032] Inside the filter box 5, at both the front and rear ends, there are two interlayers 27, ultrafiltration membranes 20, and silicone sealing rings 12. The interior of the filter box 5 is divided into internal flow channels 18 by the two interlayers 27, ultrafiltration membranes 20, and silicone sealing rings 12. Inside each of the two lids 26, an interlayer 19 is fixedly installed, dividing the interior of the lid 26 into a filtration chamber 16. After the sample enters the internal flow channels 18 of the filter box 5, it is filtered through the two ultrafiltration membranes 20, filtering out some of the solvent and small molecules in the sample, and then flowing into the waste liquid chamber 6. Furthermore, the ultrafiltration membranes 20 are placed at both the front and rear ends of the filter box 5, forming a two-part symmetrical filtration arrangement, which increases the contact area between the sample and the filter membrane, thereby increasing the filtration area and throughput.

[0033] Both ends of the filter box 5 are fixedly connected to Luer connector 2 17, and both Luer connector 2 17 are connected to the internal flow channel 18. When in use, the sample liquid flows into the internal flow channel 18 from the Luer connector 2 17 on the right side of the filter box 5. After the flow channel 18 is filled, the part that does not pass through the ultrafiltration membrane 20 flows out from the Luer connector 2 17 on the left side.

[0034] The upper end of the waste liquid chamber 6 is fixedly connected to a vent pipe 24 and two inflow pipes 23. The upper ends of the two inflow pipes 23 are respectively connected to two filter pipes 14 through pipes. A filter 25 is fixedly installed on the upper end of the vent pipe 24. The bottom ends of the vent pipe 24 and the two inflow pipes 23 are all located inside the waste liquid chamber 6. The waste liquid entering the waste liquid chamber 6 can flow into the waste liquid chamber 6 through the two filter pipes 14 and the two inflow pipes 23, and the waste liquid is collected by the waste liquid chamber 6. The filter 25 installed on the vent pipe 24 can filter impurities in the air and ensure the sterility of the concentration and filtration process of the device.

[0035] Among them, filter 19 and filter 25 are both filters with a pore size of 0.22μm and model number GVWP04700. During the sample addition process, filter 19 can filter and sterilize the sample, and at the same time remove impurities with a diameter greater than 0.22μm, playing a primary filtration role.

[0036] refer to Figure 3 The second interlayer 27 is located in the middle of the filter box 5, that is, on the side wall of the internal flow channel 18. The first interlayer 19 is located inside the box cover 26 and forms the filtration chamber 16 with the box cover 26. The materials of the first interlayer 19 and the second interlayer 27 are microporous polymer plates, which can fix the silicone sealing ring 12 and the ultrafiltration membrane 20, while dispersing the liquid pressure and guiding the liquid through the ultrafiltration membrane 20. The filtration chamber 16 plays the role of collecting the filtered waste liquid and guiding the waste liquid to be discharged through the filtration tube 14. The silicone sealing ring 12 can fill the gap between the ultrafiltration membrane 20 and the first interlayer 19, preventing the sample from leaking from the edge of the ultrafiltration membrane 20, while improving the sealing between the filter box 5 and the box cover 26 to prevent air and liquid leakage. When the buckle 13 is opened, the box cover 26 can be separated from the filter box 5, thereby exposing the silicone sealing ring 12 and the ultrafiltration membrane 20 for replacement of the ultrafiltration membrane 20.

[0037] In use, the sample is first added to the storage chamber 8 through filter 19 and sample tube 10, and then the syringe 1 is used to aspirate and push the sample into the filter box 5. When the syringe 1 aspirates, the one-way valve 22 is in the automatic open state and the one-way valve 3 is in the automatic closed state. At this time, the sample flows through the aspiration tube 21, the one-way valve 22, and the left side of the Y-type three-way valve 2 into the syringe 1 under the aspiration of the syringe 1. Then, when the syringe 1 pushes out the sample, the one-way valve 22 is in an automatic closed state and the one-way valve 3 is in an automatic open state. The sample will flow from the right channel of the Y-type three-way 2 through the one-way valve 3 and finally into the filter box 5. The flow rate of the liquid flowing out of the filter box 5 is manually controlled by the manual squeeze valve 7. When the outflow rate is reduced, a transmembrane pressure will be generated on the sample in the filter box 5, so that substances and solvents smaller than the pore size of the ultrafiltration membrane 20 in the sample pass through the filter membrane to form filtrate, while the sample flowing out of the filter box 5 is concentrated to form concentrate. After all the sample in the syringe 1 is pushed in, an appropriate amount of air can be pushed in to concentrate all the sample and flow into the storage chamber 8 through the pipe connected to the left side of the filter box 5 and the inflow pipe 11 for temporary storage.

[0038] If the volume of the concentrate is still greater than the target volume after one concentration, the above operation can be repeated to perform multiple cycles of concentration until the required final volume is reached. The storage chamber 8 is made of transparent material and has volume scale markings on its outer wall. The volume of the sample and concentrate can be measured through the scale, and the volume concentration factor can be calculated.

[0039] The reservoir 8 can be separated from the top cover 4, and then sealed with a pre-prepared sealing cap that matches the opening of the reservoir 8 for subsequent stabilization of the concentrate. Alternatively, the concentrate in the reservoir 8 can be drawn back into the syringe 1 through the aspiration tube 21, one-way valve 22, and the left channel of the Y-type three-way valve 2. Since the sample volume is concentrated, and the lower end of the aspiration tube 21 is located at the bottom of the reservoir 8, even if there is air in the tubing, the syringe 1 can aspirate the concentrate. Alternatively, a larger syringe 1 can be used to aspirate the concentrate. The concentrate can be transferred to other prepared reservoir containers by removing the syringe 1 for subsequent stabilization.

[0040] The ultrafiltration membrane 20 can be made of polyethersulfone (PES) membrane. PES membrane can provide the best balance of high throughput, low adsorption and good mechanical strength, making it very suitable for multiple cycles of concentration under the pressure of syringe 1. The specific pore size of the ultrafiltration membrane 20 depends on the user's sample type and target molecule size. Users can select the ultrafiltration membrane 20 with the appropriate pore size according to the target substance to be concentrated.

[0041] The foregoing has illustrated and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A concentrated filtration and storage device, comprising a syringe (1), a connector, a filter box (5), and a storage chamber (8), characterized in that, The connector includes a Y-type tee (2), a one-way valve (3) is provided on one side of the Y-type tee (2), a one-way valve (22) is provided on the other side of the Y-type tee (2), a filter box (5) is fixedly connected to the other end of the one-way valve (3) through a pipe, a waste liquid chamber (6) is provided below the filter box (5), a top cover (4) is provided at the upper end of the liquid storage chamber (8), and a sample tube (10), an inflow tube (11) and an outflow tube (21) are fixedly inserted into the outer wall of the top cover (4); The filter box (5) is connected to the inflow pipe (11) through a pipe, the suction pipe (21) is connected to the other end of the one-way valve (22) through a pipe, and the sample addition pipe (10) is equipped with a filter (9) at the upper end.

2. The concentrated filtration and storage device according to claim 1, characterized in that, A manual squeeze valve (7) is provided outside the pipe between the filter box (5) and the inflow pipe (11). The bottom ends of the sample addition tube (10), the inflow pipe (11) and the suction tube (21) are all located inside the liquid storage chamber (8).

3. The concentrated filtration and storage device according to claim 1, characterized in that, The filter box (5) is provided with a cover (26) at both the front and rear ends. A snap-fit ​​assembly is provided between the outer wall of the cover (26) and the outer wall of the filter box (5). The snap-fit ​​assembly includes a buckle (13) and a plate (28). The buckle (13) is rotatably connected to the outer wall of the cover (26) through a shaft. The plate (28) is fixedly installed on the outer wall of the filter box (5). The buckle (13) and the plate (28) cooperate with each other. The outer wall of the box cover (26) is fixedly connected with a Luer connector (15), and both Luer connectors (15) are fixedly connected with a filter tube (14).

4. The concentrated filtration and storage device according to claim 3, characterized in that, The filter box (5) is provided with a second interlayer (27), an ultrafiltration membrane (20) and a silicone sealing ring (12) at both the front and rear ends. The filter box (5) is divided into an internal flow channel (18) by the two second interlayers (27), the ultrafiltration membrane (20) and the silicone sealing ring (12). The two box covers (26) are fixedly installed with a first interlayer (19). The box cover (26) is divided into a filter chamber (16) by the first interlayer (19).

5. A concentrated filtration and storage device according to claim 4, characterized in that, The filter box (5) is fixedly connected to Luer connectors (17) at both ends, and both Luer connectors (17) are connected to the internal flow channel (18).

6. A concentrated filtration and storage device according to claim 5, characterized in that, The upper end of the waste liquid chamber (6) is fixedly connected to a vent pipe (24) and two inflow pipes (23), and the upper ends of the two inflow pipes (23) are respectively connected to two filter pipes (14) through pipes. A filter (25) is fixedly installed on the upper end of the vent pipe (24), and the bottom ends of the vent pipe (24) and the two inflow pipes (23) are all located inside the waste liquid chamber (6).

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