High-throughput high-precision biological cryopreservation liquid dispensing equipment

Abstract

This utility model discloses a high-throughput, high-precision biological cryopreservation solution dispensing device, comprising a cabinet. The cabinet contains at least one biological cryopreservation solution dispensing pipeline, a welding mechanism, and an integrity tester. One end of the biological cryopreservation solution dispensing pipeline is connected to a biological cryopreservation solution stock tank, and the other end is connected to a biological cryopreservation solution dispensing bag and the integrity tester. The welding mechanism is used to separate the biological cryopreservation solution dispensing bag from the biological cryopreservation solution dispensing pipeline after a predetermined amount of biological cryopreservation solution has been filled into the bag. The integrity tester is used to test the airtightness of the biological cryopreservation solution dispensing pipeline and the biological cryopreservation solution dispensing bag. The advantages of this utility model are: high degree of automation, simple operation, easy to learn, low workload, and high efficiency; high dispensing accuracy, strong applicability, no pollution, and high throughput; and low residue, which can greatly reduce the residue of the stock solution and avoid waste.

Description

Technical Field

[0001] This utility model relates to the field of biological cryopreservation solution dispensing technology, specifically to a high-throughput, high-precision biological cryopreservation solution dispensing device. Background Technology

[0002] Currently, the filling of disposable biological bags (small volume - below 50ml) is basically done by manual pipettes (a piston moves in a sealed cylinder, using compressed or expanded air to draw and discharge liquid) to ensure precise control of dispensing. This filling method is not only cumbersome to operate, but also extremely inefficient. Improper operation can easily cause the tubing to burst and cause contamination. Alternatively, some large-volume fully automated filling systems, such as filling lines for medical products like infusion bags, may be used. While this method improves efficiency, it is technically crude and often inadequate when faced with the high-throughput, high-precision dispensing requirements of precious products.

[0003] In response to this market demand, the applicant has done a great deal of technical work to achieve high-precision, high-throughput, high-efficiency, and pollution-free liquid processing technology, and has realized the above requirements one by one. Utility Model Content

[0004] The purpose of this invention is to provide a biological cryopreservation solution dispensing device that can achieve high precision, high throughput, high efficiency, and pollution-free liquid dispensing, in order to solve the technical problems existing in the background art.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows: A high-throughput, high-precision biological cryopreservation solution dispensing device includes a cabinet. The cabinet contains at least one biological cryopreservation solution dispensing pipeline, a welding mechanism, and an integrity tester. One end of the biological cryopreservation solution dispensing pipeline is connected to a biological cryopreservation solution stock tank, and the other end is connected to a biological cryopreservation solution dispensing bag and the integrity tester. The welding mechanism is used to separate the biological cryopreservation solution dispensing bag from the biological cryopreservation solution dispensing pipeline after a predetermined amount of biological cryopreservation solution has been filled into the bag. The integrity tester is used to test the airtightness of the biological cryopreservation solution dispensing pipeline and the biological cryopreservation solution dispensing bag.

[0006] Furthermore, it also includes a host computer, a slave computer, and a human-machine interface. The host computer and the slave computer are both installed inside the cabinet. The host computer is connected to the integrity tester, the slave computer, and the human-machine interface, respectively. The slave computer is connected to the biological cryopreservation solution dispensing pipeline and the welding mechanism, respectively. The human-machine interface is located on the top of the cabinet for human-machine interaction.

[0007] Furthermore, it also includes a barcode scanner and a printer, both of which are located on the outside of the left side of the cabinet and are connected to the host computer.

[0008] Furthermore, the biological cryopreservation solution dispensing pipeline includes a main pipeline and several branch pipelines. One end of the main pipeline is connected to the biological cryopreservation solution stock tank, and the other end is connected to an integrity tester and several branch pipelines. The several branch pipelines are connected to several biological cryopreservation solution dispensing bags in a one-to-one correspondence.

[0009] Furthermore, the main pipeline includes main pipeline A, main pipeline B, main pipeline C, main pipeline D, main pipeline clamp valve A, main pipeline clamp valve B, main pipeline clamp valve C, main pipeline clamp valve D, main pipeline clamp valve E, main pipeline clamp valve F, main pipeline pressure sensor A, main pipeline pressure sensor B, main pipeline bubble detection sensor A, main pipeline bubble detection sensor B, main pipeline bubble detection sensor C, main pipeline bubble detection sensor D, and a peristaltic pump; The main pipe A is sequentially clamped onto the main pipe clamp valve A, the main pipe pressure sensor A, the peristaltic pump, the main pipe pressure sensor B, the main pipe bubble detection sensor A, the main pipe clamp valve D, the main pipe bubble detection sensor D, and the main pipe clamp valve F, and is sequentially connected to the biological cryopreservation solution tank, main pipe B, main pipe C, several branch pipes, and main pipe D; main pipe B is clamped onto the main pipe clamp valve B; main pipe C is sequentially clamped onto the main pipe clamp valve C, the main pipe bubble detection sensor B, and the main pipe bubble detection sensor F. Sensor C is mounted on the main pipe; the main pipe D is clamped onto the main pipe clamp valve E and connected to the integrity tester; the main pipe clamp valves A, B, C, D, E, and F, the main pipe pressure sensor A, B, C, D, E, and D, and the peristaltic pump are all fixedly installed inside the cabinet and are all connected to the lower-level electromechanical system.

[0010] Furthermore, each of the aforementioned branch pipes includes a branch pipe, a branch pipe clamping valve, and a branch pipe bubble detection sensor. One end of the branch pipe is connected to the main pipe A, and the other end is connected to a biological cryopreservation solution dispensing bag. The branch pipe is then clamped onto the branch pipe clamping valve and the branch pipe bubble detection sensor in sequence. Both the branch pipe clamping valve and the branch pipe bubble detection sensor are fixedly installed inside the cabinet and are connected to the lower-level electromechanical system.

[0011] Furthermore, the welding mechanism includes a linear slide table and a welding head assembly disposed on the linear slide table. Both the linear slide table and the welding head assembly are electrically connected to the lower electromechanical unit. The linear slide table is used to drive the welding head assembly to move back and forth along the length direction of the linear slide table. The welding head assembly is used to automatically separate the biological cryopreservation solution dispensing bag from the biological cryopreservation solution dispensing pipeline after the predetermined amount of biological cryopreservation solution has been filled.

[0012] Furthermore, the welding head assembly includes an outer casing, a miniature electric cylinder, a limiting pin, an intermediate slider, a clamp, and an electric heating plate. The outer casing is fixedly mounted on the slider of the linear slide table. The miniature electric cylinder is fixedly mounted inside the outer casing and connected to the intermediate slider. The limiting pin is fixedly mounted inside the outer casing and passes between the intermediate slider and the clamp, serving to guide the intermediate slider and control the opening and closing of the clamp jaws. One end of the intermediate slider passes inside the outer casing, and the other end protrudes outside the outer casing, and is hinged to the clamp via a pin. Together, the jaws of the clamps are exposed outside the outer casing, and the jaws are inserted inside the middle slider. The electric heating plate is embedded in the jaws of the jaws and is used to melt and cut off the pipe connecting the biological cryopreservation solution dispensing pipeline and the biological cryopreservation solution dispensing bag, so as to separate the biological cryopreservation solution dispensing bag from the biological cryopreservation solution dispensing pipeline. A torsion spring is sleeved on the outside of the pin shaft, and the fixed foot of the torsion spring is engaged in the positioning hole on the middle slider. The movable foot of the torsion spring is engaged in the positioning hole on the clamp. The miniature electric cylinder and the electric heating plate are both electrically connected to the lower-level machine.

[0013] Furthermore, the cabinet is also equipped with a switching power supply and a cooling fan. The left side of the cabinet is equipped with a load switch, a USB port, an indicator light, an emergency stop switch, and a maintenance socket. The right side of the cabinet is equipped with a heat dissipation vent, a power interface, and a network port. The switching power supply is electrically connected to the biological cryopreservation solution dispensing pipeline, welding mechanism, host computer, slave computer, human-machine interface, indicator lights and cooling fan respectively; The load switch is electrically connected to the welding mechanism, integrity tester, printer, switching power supply, maintenance socket and power interface respectively; Both the USB interface and the network port are electrically connected to the host computer. The emergency stop switch is connected to the lower-level electromechanical system.

[0014] Furthermore, the cabinet is provided with a first observation port and a second observation port on the front side, a transparent front cabinet door is provided in front of the second observation port, a rear cabinet door is provided on the rear side of the cabinet, and casters are provided at the bottom of the cabinet. The biological cryopreservation solution dispensing pipeline installed inside the cabinet can be seen through the first and second observation ports.

[0015] Compared with the prior art, the advantages of this utility model are as follows: 1. High degree of automation, simple operation, easy to learn, reducing the workload of operators; 2. High dispensing accuracy: The peristaltic pump filling speed can be accurately measured during the dispensing process, which greatly improves the dispensing accuracy. Tests have shown that this equipment can achieve an accuracy of 0.2ml, while similar small-volume bag dispensing equipment in China generally has an accuracy of around 0.5ml. 3. Highly adaptable, suitable for various small-volume disposable packaging bags; 4. No pollution, which can eliminate most of the contamination during the repackaging process; 5. High throughput, capable of packaging multiple bags at once; 6. Low residue: This greatly reduces the amount of original solution remaining, avoiding waste. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in this embodiment, the accompanying drawings used in the description of the embodiment will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a front view of a specific embodiment of the high-throughput, high-precision biological cryopreservation fluid dispensing equipment involved in this utility model when it is in use; Figure 2 This is a three-dimensional structural schematic diagram of a specific embodiment of the high-throughput, high-precision biological cryopreservation fluid dispensing equipment involved in this utility model when it is in use, with the front cabinet door closed; Figure 3 This is a three-dimensional structural schematic diagram of a specific embodiment of the high-throughput, high-precision biological cryopreservation fluid dispensing equipment involved in this utility model when it is in use, with the rear cabinet door closed from one perspective. Figure 4 This is a front view of a specific embodiment of the high-throughput, high-precision biological cryopreservation fluid dispensing equipment involved in this utility model in a non-use state; Figure 5 This is a three-dimensional structural schematic diagram of a specific embodiment of the high-throughput, high-precision biological cryopreservation fluid dispensing equipment involved in this utility model when it is not in use, with the front cabinet door open from one perspective. Figure 6 This is a three-dimensional structural diagram of a specific embodiment of the high-throughput, high-precision biological cryopreservation fluid dispensing equipment involved in this utility model when it is not in use, with the rear cabinet door open from one perspective. Figure 7 This is a front view of a single biological cryopreservation solution dispensing pipeline and welding mechanism; Figure 8 This is a structural schematic diagram of a single biological cryopreservation solution dispensing pipeline and welding mechanism from one perspective. Figure 9 This is a structural schematic diagram of a single biological cryopreservation solution dispensing pipeline and welding mechanism from another perspective; Figure 10This is a front view of a single biological cryopreservation solution dispensing pipeline; Figure 11 This is a schematic diagram of a single biological cryopreservation solution dispensing pipeline; Figure 12 This is a schematic diagram of a single welding mechanism; Figure 13 This is a structural schematic diagram of a single welding head assembly; Figure 14 yes Figure 13 A magnified view of a portion of the image; Figure 15 This is a schematic diagram of the internal structure of a single welding head assembly. Figure 1 ; Figure 16 This is a schematic diagram of the internal structure of a single welding head assembly. Figure 2 ; Figure 17 This is a schematic diagram of the internal structure of a single welding head assembly. Figure 3 ; Figure 18 yes Figure 1 Working principle diagram of the high-throughput, high-precision biological cryopreservation solution dispensing equipment involved in the embodiment; Explanation of reference numerals in the attached diagram: 1. Cabinet; 2. Biological cryopreservation solution dispensing pipeline; 210. Main pipeline; 2101. Main pipeline A; 2102. Main pipeline B; 2103. Main pipeline C; 2104. Main pipeline D; 2105. Main pipeline pinch valve A; 2106. Main pipeline pinch valve B; 2107. Main pipeline pinch valve C; 2108. Main pipeline pinch valve D; 2109. Main pipeline pinch valve E; 2110. Main pipeline pinch valve F; 2111. Main pipeline pressure sensor A; 2112. Main pipeline pressure sensor B; 2113. Main pipeline air bubble detection sensor A; 2114. Main pipeline air bubble detection sensor B; 2115. Main pipeline air bubble detection sensor C; 2116. Main pipeline air bubble detection sensor D; 2117. Peristaltic pump; 2118. None 1. Microbial filter; 2119. Pipe clamp; 220. Branch pipe; 2201. Branch pipe; 2202. Branch pipe clamp valve; 2203. Branch pipe air bubble detection sensor; 3. Biological cryopreservation solution dispensing bag; 4. Welding mechanism; 410. Linear slide; 420. Welding head assembly; 5. Integrity tester; 6. Upper computer; 7. Lower computer; 8. Human-machine interface; 9. Barcode scanner; 10. Printer; 11. Switching power supply; 12. Cooling fan; 13. Load switch; 14. USB interface; 15. Indicator light; 16. Emergency stop switch; 17. Maintenance socket; 18. Heat dissipation vent; 19. Power interface; 20. Network port; 21. First observation port; 22. Second observation port; 23. Transparent front cabinet door; 24. Rear cabinet door; 25. Casters; 26. Bracket. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can typically be arranged and designed in various different configurations.

[0019] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0020] In the description of the embodiments of this utility model, it should be noted that if terms such as "upper," "lower," "horizontal," or "inner" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this utility model. Furthermore, terms such as "first" and "second" are only used to distinguish descriptions and should not be construed as indicating or implying relative importance. In addition, the appearance of the term "horizontal" does not mean that the component is required to be absolutely horizontal, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but rather that it can be slightly tilted.

[0021] In the description of the embodiments of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0022] The present invention will be further described below with reference to the accompanying drawings: See Figures 1 to 18This is a specific embodiment of a high-throughput, high-precision biological cryopreservation solution dispensing device provided by this utility model. Specifically, this embodiment includes a cabinet 1, inside which are arranged two biological cryopreservation solution dispensing pipes 2, two welding mechanisms 4, and two integrity testers 5. The two biological cryopreservation solution dispensing pipes 2 are arranged side by side, one above the other, inside the cabinet 1. The two welding mechanisms 4 and the two integrity testers 5 are also arranged side by side, one above the other, inside the cabinet 1, located behind the two biological cryopreservation solution dispensing pipes 2 and corresponding one-to-one with the two biological cryopreservation solution dispensing pipes 2. That is, each biological cryopreservation solution dispensing pipe 2 is matched with one welding mechanism 4 and one integrity tester 5 to work together.

[0023] Specifically, in the above specific embodiments, one end of each cryopreservation solution dispensing pipe 2 is connected to the corresponding cryopreservation solution stock tank (not shown in the figure), and the other end is connected to the corresponding cryopreservation solution dispensing bag 3 and integrity tester 5; each cryopreservation solution dispensing pipe 2 is used to dispense the cryopreservation solution stock from the corresponding cryopreservation solution stock tank into the corresponding cryopreservation solution dispensing bag 3, thereby realizing the dispensing of cryopreservation solution stock; each welding mechanism 4 is used to separate the corresponding cryopreservation solution dispensing bag 3 from the corresponding cryopreservation solution dispensing pipe 2 after filling the corresponding cryopreservation solution dispensing bag 3 with a predetermined amount of cryopreservation solution; each integrity tester 4 is used to test the airtightness of the corresponding cryopreservation solution dispensing pipe 2 and the cryopreservation solution dispensing bag 3 to avoid leakage during subsequent liquid dispensing.

[0024] It should be understood here that the number of biological cryopreservation solution dispensing tubing 2, welding mechanism 4, and integrity tester 5 can be one or more, depending on actual needs. Furthermore, it should be noted that when there are multiple biological cryopreservation solution dispensing tubing 2, these multiple tubing 2 can either be connected together to a single biological cryopreservation solution bulk tank, or each can be connected to a separate biological cryopreservation solution bulk tank. That is, N biological cryopreservation solution dispensing tubing 2 correspond to N biological cryopreservation solution bulk tanks, meaning each tubing 2 is connected to one bulk tank without interference. When multiple biological cryopreservation solution dispensing tubing 2 are connected together to a single bulk tank, they are used for simultaneously dispensing the same type of cryopreservation solution. The cryopreservation solution (i.e., the cryopreservation solution entering each cryopreservation solution dispensing line 2 comes from the same cryopreservation solution stock tank); when multiple cryopreservation solution dispensing lines 2 are each connected to a cryopreservation solution stock tank, they can be used to dispense the same type of cryopreservation solution at the same time, or they can be used to dispense different types of cryopreservation solutions at the same time, depending on whether the liquids in each cryopreservation solution stock tank are the same; if they are the same, they are used to dispense the same type of cryopreservation solution at the same time, and if they are different, they are used to dispense different types of cryopreservation solutions at the same time.

[0025] Please continue reading. Figures 1 to 6 , Figure 18 The high-throughput, high-precision biological cryopreservation solution dispensing equipment provided by this utility model also includes a host computer 6, a slave computer 7, and a human-machine interface 8. The host computer 6 and the slave computer 7 are both installed inside the cabinet 1. The host computer 6 is connected to the integrity tester 5, the slave computer 7, and the human-machine interface 8, respectively, and is used to control the integrity tester 5, the slave computer 7, and the human-machine interface 8 to perform corresponding operations. The slave computer 7 is connected to the biological cryopreservation solution dispensing pipeline 2 and the welding mechanism 4, respectively, and is used to control the pipeline control elements and pipeline detection elements in the biological cryopreservation solution dispensing pipeline 2 to perform corresponding operations. The human-machine interface 8 is installed on the top of the cabinet 1 for human-machine interaction.

[0026] Specifically, the integrity tester 5 is a proprietary product of our company and is existing technology, with the specific model number SKC0653-0001.

[0027] Specifically, the host computer 6 is an industrial control computer, which is existing technology, and can specifically be an Advantech industrial control computer; the host computer 6 is the core control component of this equipment, used to control the operation of the entire equipment.

[0028] Specifically, the lower-level machine 7 is a PLC controller, which is existing technology. It can be a Siemens S7-200 series PLC, or a Siemens S7-300 series PLC, S7-400 series PLC, or S7-1200 series PLC. The lower-level machine 7 is controlled by the upper-level machine 6 and is used to perform corresponding actions on the actuators in the biological cryopreservation liquid dispensing pipeline 2 and the welding mechanism 4.

[0029] Specifically, the human-machine interface 8 is an LCD screen controlled by the host computer 6, used to display the corresponding working interface and interact with the operator.

[0030] Please continue reading. Figures 1 to 6 , Figure 18 The high-throughput, high-precision biological cryopreservation solution dispensing equipment provided by this utility model also includes a barcode scanner 9 and a printer 10, both of which are located on the left side of the cabinet 1 and connected to the host computer 6. The barcode scanner 9 is used to scan the QR code (including information such as date, material name, and weight) affixed to the biological cryopreservation solution dispensing bag 3 and automatically uploads the scanned QR code information to the host computer 6, which then automatically enters the corresponding biological cryopreservation solution dispensing bag 3 information. The printer 10 is used to print the QR code.

[0031] Specifically, in this utility model, see... Figures 7 to 11Each biological cryopreservation solution dispensing pipeline 2 includes a main pipeline 210 and several branch pipelines 220. One end of the main pipeline 210 is connected to the corresponding biological cryopreservation solution stock tank, and the other end is connected to the corresponding integrity tester 5 and several branch pipelines 220. Several branch pipelines 220 are connected to several biological cryopreservation solution dispensing bags 3 in a one-to-one correspondence.

[0032] Specifically, as an optional embodiment of main road 210, see [reference] Figure 10 and Figure 11The main pipeline 210 includes main pipeline A2101, main pipeline B2102, main pipeline C2103, main pipeline D2104, main pipeline pinch valve A2105, main pipeline pinch valve B2106, main pipeline pinch valve C2107, main pipeline pinch valve D2108, main pipeline pinch valve E2109, main pipeline pinch valve F2110, main pipeline pressure sensor A2111, main pipeline pressure sensor B2112, main pipeline bubble detection sensor A2113, main pipeline bubble detection sensor B2114, main pipeline bubble detection sensor C2115, main pipeline bubble detection sensor D2116, and peristaltic pump 2117; wherein, main pipeline A2101 is sequentially clamped between the main pipeline pinch valves. A2105, main line pressure sensor A2111, peristaltic pump 2117, main line pressure sensor B2112, main line bubble detection sensor A2113, main line pinch valve D2108, main line bubble detection sensor D2116, and main line pinch valve F2110 are connected in sequence to the biological cryopreservation solution tank, main line B2102, main line C2103, several branch lines 220, and main line D2104; main line B2102 is clamped onto main line pinch valve B2106; main line C2103 is clamped in sequence onto main line pinch valve C2107, main line bubble detection sensor B2114, and main line bubble detection sensor C2115; main line D210... 4 is clamped onto the main pipeline clamp valve E2109 and connected to the integrity tester 5; the main pipeline clamp valves A2105, B2106, C2107, D2108, E2109, and F2110, the main pipeline pressure sensor A2111, B2112, A2113, B2114, C2115, and D2116, and the peristaltic pump 2117 are all fixedly installed inside the cabinet 1 and are all electrically connected to the lower-level machine 7; the main pipeline clamp valve A2105, the main pipeline clamp valve... Pipe clamp valves B2106, C2107, D2108, E2109, and F2110 are each used to control the liquid flow in their respective pipes; pipe pressure sensors A2111 and B2112 are used to detect the pressure in the pipes before and after the peristaltic pump 2117; pipe bubble detection sensors A2113, B2114, C2115, and D2116 are used to detect the bubble situation in their respective pipes, thereby inferring the liquid level change in the pipes; the peristaltic pump 2117 is used to control the liquid flow speed and direction in the pipes.

[0033] Specifically, in the above-mentioned embodiment of the main pipeline 210, the main pipeline clamp valves A2105, B2106, C2107, D2108, E2109 and F2110 are all preferably Sihan Intelligent PVM192B series four-wire electric clamp valves.

[0034] Specifically, in the above embodiment of the main pipeline 210, both the main pipeline pressure sensor A2111 and the main pipeline pressure sensor B2112 are preferably pressure sensors of model FX1900 from Chengdu Miaoli Technology Co., Ltd.

[0035] Specifically, in the above-mentioned embodiment of the main pipeline 210, the main pipeline bubble detection sensor A2113, the main pipeline bubble detection sensor B2114, the main pipeline bubble detection sensor C2115 and the main pipeline bubble detection sensor D2116 are all preferably ultrasonic bubble detector sensors of model MU08.4 from Chengdu Miaoli Technology Co., Ltd.

[0036] Specifically, in the above-mentioned embodiment of main pipeline 210, the peristaltic pump 2117 can be any commercially available small peristaltic pump, such as the peristaltic pump model dPOFLEX BPHOl from Baoding Lange Constant Flow Pump Co., Ltd.

[0037] Furthermore, in the above-described embodiment of the main pipeline 210, see [reference]. Figure 10 and Figure 11 Sterile filters 2118 are also installed at the ends of main pipes A2101, B2102, C2103 and D2104 to isolate bacteria and impurities in the air, maintain stable pressure inside the pipes, ensure unidirectional flow of liquid and avoid back contamination.

[0038] Furthermore, in the above-described embodiment of the main pipeline 210, see [reference]. Figure 10 and Figure 11 Both main pipe A2101 and main pipe C2103 are clamped with pipe clamps 2119; the pipe clamps 2119 help to fix main pipe A2101 and main pipe C2103 and ensure that the liquid in main pipe A2101 and main pipe C2103 flows smoothly.

[0039] Furthermore, in the above-mentioned embodiment of the main pipeline 210, apart from the clamp valves, pressure sensors, bubble detection sensors and peristaltic pumps, the remaining components are all disposable items that can be disassembled and replaced with new items after each use to prevent cross-contamination of the pipeline.

[0040] Specifically, as an optional embodiment of branch pipe 220, see [reference] Figure 10 and Figure 11The branch pipe 220 includes a branch pipe 2201, a branch pipe clamp valve 2202, and a branch pipe bubble detection sensor 2203. One end of the branch pipe 2201 is connected to the main pipe A2101, and the other end is connected to the biological cryopreservation solution dispensing bag 3. It is clamped onto the branch pipe clamp valve 2202 and the branch pipe bubble detection sensor 2203 in sequence. The branch pipe clamp valve 2202 and the branch pipe bubble detection sensor 2203 are both fixedly installed in the cabinet 1 and electrically connected to the lower-level machine 7. The branch pipe clamp valve 2202 is used to control the flow of liquid in the branch pipe. The branch pipe bubble detection sensor 2203 is used to detect the liquid level change in the branch pipe.

[0041] Specifically, in the above-mentioned branch pipe 220 embodiment, the branch pipe clamp valve 2202 is preferably a Sihan Intelligent PVM192B series four-wire electric clamp valve; the branch pipe bubble detection sensor 2203 is preferably an ultrasonic bubble detector sensor of model MU08.4 from Chengdu Miaoli Technology Co., Ltd.

[0042] Furthermore, in the above-mentioned branch pipe 220 embodiment, apart from the branch pipe clamp valve 2202 and the branch pipe bubble detection sensor 2203, the other components are disposable items that can be disassembled and replaced with new items after each use to prevent cross-contamination of the pipes.

[0043] Specifically, as an optional embodiment of welding mechanism 4, see [reference]. Figure 12 The welding mechanism 4 includes a linear slide 410 and a welding head assembly 420 disposed on the linear slide 410. Both the linear slide 410 and the welding head assembly 420 are electrically connected to the lower computer 7. The linear slide 410 is used to drive the welding head assembly 420 to move back and forth along the length of the linear slide 410. The welding head assembly 420 is used to automatically separate the biological cryopreservation solution dispensing bag 3 from the biological cryopreservation solution dispensing pipeline 2 after the predetermined amount of biological cryopreservation solution has been filled.

[0044] Specifically, the linear slide 410 adopts existing known technologies, such as single-axis ball screw linear module slides (such as the SDM series of Dongguan Shihai Automation Technology Co., Ltd.) or single-axis synchronous belt linear module slides (such as the TA series of Dongguan Shihai Automation Technology Co., Ltd.).

[0045] Specifically, as an optional embodiment of the welding head assembly 420, see [reference]. Figures 13 to 17The welding head assembly 420 includes an outer housing 4201, a miniature electric cylinder 4202, a limiting pin 4203, an intermediate slider 4204, a clamp 4205, and an electric heating plate 4206. The outer housing 4201 is fixedly mounted on the slider of the linear slide table 410. The miniature electric cylinder 4202 is fixedly mounted inside the outer housing 4201 and connected to the intermediate slider 4204. The limiting pin 4203 is fixedly mounted inside the outer housing 4201 and passes between the intermediate slider 4204 and the clamp 4205, serving to guide the intermediate slider 4204 and control the opening and closing of the clamp jaws 4205. One end of the intermediate slider 4204 passes inside the outer housing 4201, and the other end protrudes outside the outer housing 4201 and is hinged to the clamp 4205 via a pin 4207. The clamp head is exposed outside the outer casing 4201, and the clamp tail is inserted inside the middle slider 4204. The electric heating plate 4206 is embedded in the jaw of the clamp head and is used to melt and cut off the pipe connecting the biological cryopreservation solution dispensing pipe 2 and the biological cryopreservation solution dispensing bag 3, so that the biological cryopreservation solution dispensing bag 3 is separated from the biological cryopreservation solution dispensing pipe 2. A torsion spring 4208 is sleeved on the outside of the pin shaft 4207, and the fixed foot of the torsion spring 4208 is engaged in the positioning hole on the middle slider 4204. The movable foot of the torsion spring 4208 is engaged in the positioning hole on the clamp 4205. The torsion spring 4208 is used to automatically reset the clamp 4205 when the clamp tail of the clamp 4205 leaves the limit pin 4203, so that the clamp jaw of the clamp 4205 automatically opens. The miniature electric cylinder 4202 and the electric heating plate 4206 are both electrically connected to the lower computer 7.

[0046] Furthermore, in the aforementioned welding head assembly 420, see [reference] Figure 14 There are two electric heating plates 4206, which are located on the left and right sides of the jaws of the clamp 4205. On the opposite side of the two electric heating plates 4206, there are three protrusions: upper, middle and lower. The middle protrusion is pointed and sharp, used to cut the pipe, while the two protrusions on the sides are flat, used to weld the pipe.

[0047] The working principle of the above-described welding head assembly 420 embodiment is as follows: When the welding head assembly 420 needs to weld the pipeline (i.e., the branch pipe 2201 connecting the biological cryopreservation bag 3), refer to... Figure 16First, the welding head assembly 420 is moved to the rear side of the pipe to be welded via the linear slide 410. Then, the intermediate slider 4204 is extended from the outer casing 4201 via the miniature electric cylinder 4202. During the extension of the intermediate slider 4204 from the outer casing 4201, the clamp 4205 moves forward along with it until the rear end of the intermediate slider 4204 abuts against the limiting pin 4203. At this point, the tail of the clamp 4205 opens outward under the action of the limiting pin 4203, while the head of the clamp 4205 (i.e., the clamping jaw) closes, thus clamping the pipe to be welded. The pipe is clamped, and then the electric heating plate 4206 is activated to generate heat. The high temperature generated by the electric heating plate 4206 welds and melts the pipe clamped by the clamp 4205, separating the biological cryopreservation solution dispensing bag 3 from the corresponding branch pipe 220. After the pipe welding is completed, the intermediate slider 4204 is retracted into the outer casing 4201 by the micro-cylinder 4202. As the intermediate slider 4204 extends from the outer casing 4201, the clamp 4205 moves backward along with it until the intermediate slider 4204 returns to its initial position. (See reference...) Figure 15 As shown; when the miniature electric cylinder 4202 just begins to pull the intermediate slider 4204 back into the outer casing 4201, the rear end of the intermediate slider 4204 and the tail end of the clamp 4205 will simultaneously leave the blocking action of the limiting pin 4203. When the tail end of the clamp 4205 loses the blocking action of the limiting pin 4203, the tail end of the clamp 4205 will return to the initial state under the action of the torsion spring 4208. At this time, the head of the clamp 4205 (i.e., the clamping part) will open, release the pipe, and then move backward together with the intermediate slider 4204 until the intermediate slider 4204 returns to the initial position.

[0048] See Figure 6 and Figure 18The high-throughput, high-precision cryopreservation fluid dispensing equipment provided in this embodiment of the invention includes a switching power supply 11 and a cooling fan 12 inside the cabinet 1. The left side of the cabinet 1 also includes a load switch 13, a USB interface 14, an indicator light 15, an emergency stop switch 16, and a maintenance socket 17. The right side of the cabinet 1 includes a heat dissipation vent 18, a power interface 19, and a network port 20. The switching power supply 11 is connected to each clamping valve, pressure sensor, bubble detection sensor, peristaltic pump, welding mechanism 4, upper computer 6, lower computer 7, human-machine interface 8, indicator light 15, and cooling fan in the cryopreservation fluid dispensing pipeline 2. 12 Electrical connections are provided. Switching power supply 11 provides a stable 24V power supply to these electronic components. Load switch 13 is electrically connected to welding mechanism 4, integrity tester 5, printer 10, switching power supply 11, maintenance socket 17, and power interface 19, respectively, to control the circuit switching of the entire device. USB interface 1 and network port 20 are both electrically connected to host computer 6, and emergency stop switch 16 is electrically connected to slave computer 7. Cooling fan 12 is used to dissipate heat from the control cabinet. Load switch 13 is the main power switch, used to control the circuit switching of the entire device. USB interface 14 is a socket for external devices such as mice and USB flash drives. Indicator light 15 indicates power on / off. Emergency stop switch 16 is used to stop slave computer 7 in case of emergency. Maintenance socket 17 provides temporary power support for maintenance personnel. Vent 18 is used for ventilation and heat dissipation. Power interface 19 is used to connect to external 220V AC power to provide power transmission for the entire device. Network port 20 is used to connect to external network cable to realize data transmission.

[0049] Specifically, in the above specific embodiments, see [reference] Figure 5 The cabinet 1 has a first observation port 21 and a second observation port 22 on the front side. A transparent front cabinet door 23 is provided in front of the second observation port 22. Through the transparent front cabinet door 23 in front of the first observation port 21 and the second observation port 22, the biological cryopreservation liquid dispensing pipeline 2 installed in the cabinet 1 can be seen.

[0050] Specifically, in the above specific embodiments, see [reference] Figure 4 The cabinet 1 is equipped with a rear door 24, which makes it easy to open the cabinet and install and maintain the electrical equipment inside the cabinet 1; the bottom of the cabinet 1 is equipped with casters 25 (such as casters) to facilitate the movement of the entire equipment.

[0051] Specifically, in the above specific embodiments, see [reference] Figure 1 , Figure 2 , Figure 4 and Figure 5 The cabinet 1 is also equipped with a bracket 26, which includes multiple partitions. A partition cavity is provided between two adjacent partitions. Each partition cavity is used to separate and support a biological cryopreservation solution dispensing bag 3.

[0052] The working process of the high-throughput, high-precision biological cryopreservation solution dispensing equipment provided in this embodiment of the invention is as follows: 1. Create batch number: Create a packaging batch on the human-machine interface 8.

[0053] 2. Piping Installation: First, install the pre-fabricated dispensing piping assembly (consisting of main pipe A2101, main pipe B2102, main pipe C2103, main pipe D2104, several branch pipes 2201, and several biological cryopreservation solution dispensing bags 3) onto the self-contained branch piping control assembly (consisting of each main pipe clamp valve, each main pipe pressure sensor, each main pipe bubble detection sensor, each branch pipe clamp valve, each branch pipe bubble detection sensor, and peristaltic pump) inside cabinet 1; then connect the front end of the installed dispensing piping assembly (i.e., the front end of main pipe A2101) to the biological cryopreservation solution stock tank using a sterile connector, and connect the rear end (i.e., the rear end of main pipe D2104) to the integrity tester 5.

[0054] 3. Recipe Download: On the human-machine interface 8, the recipe is downloaded from the recipe table of the host computer 6 (recipe parameters include: dispensing quantity, dispensing weight, dispensing speed, etc.).

[0055] 4. Scan QR code: Use barcode scanner 9 to scan the QR code affixed to each repackaging bag (i.e., biological cryopreservation solution repackaging bag 3) and transmit it to the host computer 6. The host computer 6 will then automatically enter the information scanned by barcode scanner 9 into the system.

[0056] 5. Integrity Test: First, close the main pipe clamp valves A2105, B2106, C2107, and F2110 on the main pipe 210, and open the branch pipe clamp valves 2203 on all branch pipes 220. Then, the integrity tester 5 introduces gas into the biological cryopreservation solution dispensing pipeline 2 and the biological cryopreservation solution dispensing bag 3 from the air inlet of the integrity tester (i.e., the end of the main pipe D2104), and maintains the pressure inside the biological cryopreservation solution dispensing pipeline 2 and the biological cryopreservation solution dispensing bag 3 (the pressure is detected by the integrity tester 5, and the data is fed back to the host computer 6) at 40~50 mbar for 2 minutes. If the pressure is stable, it indicates that the pressure holding is successful; if the pressure drops, it indicates that the biological cryopreservation solution dispensing pipeline 2... The cryopreservation solution dispensing bag 3 is defective and needs to be replaced. After the pressure holding is passed, the integrity tester 5 draws air from the air inlet (i.e., the end of the main pipe D2104) until the pressure inside the cryopreservation solution dispensing pipeline 2 and the cryopreservation solution dispensing bag 3 (the pressure is detected by the integrity tester 5 and the data is fed back to the host computer 6) is around -10 mbar, then stops drawing air. Then, the main pipe clamp valves B2106, C2107, and F2110 are opened and held for about 10 seconds to equalize the pressure inside the cryopreservation solution dispensing pipeline 2 and the cryopreservation solution dispensing bag 3 (i.e., to allow the pressure inside the cryopreservation solution dispensing pipeline 2 and the cryopreservation solution dispensing bag 3 to be connected to the atmosphere). Finally, all valves are closed, and the integrity test is completed.

[0057] 6. Main line filling: First, open the main line clamp valves A2105, D2108, and F2110. Then, turn on the peristaltic pump 2117 to start filling the main line 210 with the concentrate. At the same time, the liquid in the main line is detected by the main line bubble detection sensor D2116. When the main line bubble detection sensor D2116 detects bubbles, turn off the peristaltic pump 2117 and the main line clamp valves D2108 and F2110. The filling of the main line 210 is complete.

[0058] 7. Speed ​​Measurement: First, open the main pipeline clamp valve C2107, then turn on the peristaltic pump 2117 to allow the liquid to flow into the main pipeline C2103. Next, turn off the peristaltic pump 211. The host computer 7 obtains the time between the liquid flowing through the main pipeline bubble detection sensor B2114 and the main pipeline bubble detection sensor C2115. The filling speed of the peristaltic pump 2117 is then calculated. Finally, close the main pipeline clamp valve C2107. The speed measurement is now complete.

[0059] 8. Dispensing: First, open the main pipeline clamp valve A2105, the main pipeline clamp valve D2108, and the branch pipeline clamp valve 2202 on the first branch pipeline 220. Then, turn on the peristaltic pump 2117 to start filling. The liquid flows into the biological cryopreservation solution dispensing bag 3 on the first branch pipeline 220. When the specified volume is reached (determined by the branch pipeline air bubble detection sensor 2203 on the first branch pipeline 220), close the branch pipeline clamp valve 2202 on the first branch pipeline 220. Then, open the branch pipeline clamp valve 2202 on the second branch pipeline 220 to start filling the biological cryopreservation solution dispensing bag 3 on the second branch pipeline 220. After filling to the designated volume (determined by the branch line air bubble detection sensor 2203 on the second branch line 220), close the branch line clamp valve 2202 on the second branch line 220, and then open the branch line clamp valve 2202 on the third branch line 220 to begin filling the biological cryopreservation solution dispensing bag 3 on the third branch line 220. This process is repeated for the remaining biological cryopreservation solution dispensing bags 3 until all biological cryopreservation solution dispensing bags 3 on all branch lines 220 are filled. Finally, close all clamp valves and turn off the peristaltic pump to complete the dispensing process.

[0060] 9. Venting: First, open the main pipe clamp valves A2105, D2108, and F2110. Then, turn on the peristaltic pump 2117 to reverse and pump back the liquid in the main pipe 210 until the main pipe bubble detection sensor A2113 detects no bubbles in the main pipe A2101. Next, close the main pipe clamp valves D2108 and F2110, open the main pipe clamp valve C2107, and continue pumping back the liquid in the main pipe C2103 until the main pipe bubble detection sensor A2113 detects no bubbles in the main pipe A2101 again. Delay the peristaltic pump 2117 to reverse and pump back the liquid in the main pipe 210 for about 20 seconds to ensure that the entire main pipe 210 is free of liquid. Then, close all valves and the peristaltic pump 2117. Afterward, start... Open the main pipeline clamp valve B2106, the main pipeline clamp valve D2108, and the branch pipeline clamp valve 2202 on the first branch pipeline 220. Then, turn on the peristaltic pump 2117 to rotate forward. Through its own weight and the compressed air from the peristaltic pump 2117, the liquid in the first branch pipeline 220 is pumped into the corresponding biological cryopreservation solution dispensing bag 3. Then, close the branch pipeline clamp valve 2202 on the first branch pipeline 220 and open the branch pipeline clamp valve 2202 on the second branch pipeline 220 to pump the liquid in the second branch pipeline 220 into the corresponding biological cryopreservation solution dispensing bag 3. Continue in this manner to pump the remaining biological cryopreservation solution dispensing bags 3 until all the liquid in all the branch pipelines has been pumped into the corresponding biological cryopreservation solution dispensing bags 3. Finally, close all valves to complete the dispensing process.

[0061] 10. Welding: Start the linear slide 410, first move the welding head assembly 420 to the position of the first branch pipe 220, and then perform the welding operation on the first branch pipe 220 to separate the first branch pipe 220 from the corresponding biological cryopreservation solution dispensing bag 3; then move the welding head assembly 420 to the position of the second branch pipe 220 and perform the welding operation on the second branch pipe 220 to separate the second branch pipe 220 from the corresponding biological cryopreservation solution dispensing bag 3, and so on, to complete the welding work of the remaining branch pipes 220, so that each branch pipe 220 is separated from its corresponding biological cryopreservation solution dispensing bag 3.

[0062] Finally, it should be noted that the above description is only an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A high-throughput, high-precision biological cryopreservation solution dispensing device, comprising a cabinet (1), characterized in that: The cabinet (1) is equipped with at least one biological cryopreservation solution dispensing pipeline (2), a welding mechanism (4) and an integrity tester (5). One end of the biological cryopreservation solution dispensing pipeline (2) is connected to the biological cryopreservation solution stock tank, and the other end is connected to the biological cryopreservation solution dispensing bag (3) and the integrity tester (5). The welding mechanism (4) is used to separate the biological cryopreservation solution dispensing bag (3) from the biological cryopreservation solution dispensing pipeline (2) after a predetermined amount of biological cryopreservation solution has been filled into the biological cryopreservation solution dispensing bag (3). The integrity tester (5) is used to test the airtightness of the biological cryopreservation solution dispensing pipeline (2) and the biological cryopreservation solution dispensing bag (3).

2. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 1, characterized in that: It also includes a host computer (6), a slave computer (7) and a human-machine interface (8). The host computer (6) and the slave computer (7) are both located inside the cabinet (1). The host computer (6) is connected to the integrity tester (5), the slave computer (7) and the human-machine interface (8) respectively. The slave computer (7) is connected to the biological cryopreservation solution dispensing pipeline (2) and the welding mechanism (4) respectively. The human-machine interface (8) is located on the top of the cabinet (1) for human-machine interaction.

3. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 2, characterized in that: It also includes a barcode scanner (9) and a printer (10), both of which are located on the left side of the cabinet (1) and are connected to the host computer (6).

4. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 2 or 3, characterized in that: The biological cryopreservation solution dispensing pipeline (2) includes a main pipeline (210) and several branch pipelines (220). One end of the main pipeline (210) is connected to the biological cryopreservation solution stock tank, and the other end is connected to the integrity tester (5) and several branch pipelines (220). The several branch pipelines (220) are connected to several biological cryopreservation solution dispensing bags (3) in a one-to-one correspondence.

5. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 4, characterized in that: The main pipeline (210) includes main pipeline A (2101), main pipeline B (2102), main pipeline C (2103), main pipeline D (2104), main pipeline clamp valve A (2105), main pipeline clamp valve B (2106), main pipeline clamp valve C (2107), main pipeline clamp valve D (2108), main pipeline clamp valve E (2109), main pipeline clamp valve F (2110), main pipeline pressure sensor A (2111), main pipeline pressure sensor B (2112), main pipeline bubble detection sensor A (2113), main pipeline bubble detection sensor B (2114), main pipeline bubble detection sensor C (2115), main pipeline bubble detection sensor D (2116), and peristaltic pump (2117). Among them, main pipe A (2101) is sequentially clamped onto main pipe clamp valve A (2105), main pipe pressure sensor A (2111), peristaltic pump (2117), main pipe pressure sensor B (2112), main pipe bubble detection sensor A (2113), main pipe clamp valve D (2108), main pipe bubble detection sensor D (2116), and main pipe clamp valve F (2110), and is sequentially connected to the biological cryopreservation liquid stock tank, main pipe B (2102), main pipe C (2103), several branch pipes (220), and main pipe D (2104); main pipe B (2102) is clamped onto main pipe clamp valve B (2106); main pipe C (2103) is sequentially clamped onto main pipe clamp valve C (2107), main pipe bubble detection sensor B (2114), and main pipe bubble detection sensor F (2115). The main pipe is clamped on the sensor C (2115); the main pipe D (2104) is clamped on the main pipe clamp valve E (2109) and connected to the integrity tester (5); the main pipe clamp valve A (2105), the main pipe clamp valve B (2106), the main pipe clamp valve C (2107), the main pipe clamp valve D (2108), the main pipe clamp valve E (2109), the main pipe clamp valve F (2110), the main pipe pressure sensor A (2111), the main pipe pressure sensor B (2112), the main pipe bubble detection sensor A (2113), the main pipe bubble detection sensor B (2114), the main pipe bubble detection sensor C (2115), the main pipe bubble detection sensor D (2116) and the peristaltic pump (2117) are all fixedly installed in the cabinet (1) and are all electrically connected to the lower computer (7).

6. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 5, characterized in that: Each of the branch pipes (220) includes a branch pipe (2201), a branch pipe clamp valve (2202), and a branch pipe bubble detection sensor (2203). One end of the branch pipe (2201) is connected to the main pipe A (2101), and the other end is connected to a biological cryopreservation solution dispensing bag (3). The branch pipe (2201) is clamped in sequence on the branch pipe clamp valve (2202) and the branch pipe bubble detection sensor (2203). The branch pipe clamp valve (2202) and the branch pipe bubble detection sensor (2203) are both fixedly installed in the cabinet (1) and are electrically connected to the lower computer (7).

7. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 1, characterized in that: The welding mechanism (4) includes a linear slide (410) and a welding head assembly (420) mounted on the linear slide (410). Both the linear slide (410) and the welding head assembly (420) are electrically connected to the lower computer (7). The linear slide (410) is used to drive the welding head assembly (420) to move back and forth along the length of the linear slide (410). The welding head assembly (420) is used to automatically separate the biological cryopreservation solution dispensing bag (3) from the biological cryopreservation solution dispensing pipeline (2) after the predetermined amount of biological cryopreservation solution has been filled.

8. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 7, characterized in that: The welding head assembly (420) includes an outer shell (4201), a miniature electric cylinder (4202), a limiting pin (4203), an intermediate slider (4204), a clamp (4205), and an electric heating plate (4206). The outer shell (4201) is fixedly mounted on the slider of the linear slide (410). The miniature electric cylinder (4202) is fixedly mounted inside the outer shell (4201) and connected to the intermediate slider (4204). The limiting pin (4203) is fixedly mounted inside the outer shell (4201) and passes between the intermediate slider (4204) and the clamp (4205) to guide the intermediate slider (4204) and control the opening and closing of the clamp jaws (4205). One end of the intermediate slider (4204) passes inside the outer shell (4201), and the other end protrudes outside the outer shell (4201). It is hinged to the clamp (4205) by a pin (4207). The clamp head of the clamp (4205) is exposed outside the outer cover (4201), and the clamp tail is inserted inside the middle slider (4204). The electric heating plate (4206) is embedded in the jaw of the clamp head and is used to melt the pipe connecting the biological cryopreservation liquid dispensing pipeline (2) and the biological cryopreservation liquid dispensing bag (3) to separate the biological cryopreservation liquid dispensing bag (3) from the biological cryopreservation liquid dispensing pipeline (2). A torsion spring (4208) is sleeved on the outside of the pin (4207), and the fixed foot of the torsion spring (4208) is inserted into the positioning hole on the middle slider (4204). The movable foot of the torsion spring (4208) is inserted into the positioning hole on the clamp (4205). The micro electric cylinder (4202) and the electric heating plate (4206) are both electrically connected to the lower machine (7).

9. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 3, characterized in that: The cabinet (1) is also equipped with a switching power supply (11) and a cooling fan (12). The left side of the cabinet (1) is also equipped with a load switch (13), a USB interface (14), an indicator light (15), an emergency stop switch (16) and a maintenance socket (17). The right side of the cabinet (1) is equipped with a heat dissipation vent (18), a power interface (19) and a network port (20). The switching power supply (11) is electrically connected to the biological cryopreservation solution dispensing pipeline (2), welding mechanism (4), host computer (6), slave computer (7), human-machine interface (8), indicator light (15) and cooling fan (12), respectively. The load switch (13) is electrically connected to the welding mechanism (4), integrity tester (5), printer (10), switching power supply (11), maintenance socket (17) and power interface (19), respectively. The USB interface (14) and the network port (20) are both electrically connected to the host computer (6); The emergency stop switch (16) is electrically connected to the lower-level machine (7).

10. The high-throughput, high-precision biological cryopreservation solution dispensing equipment according to claim 9, characterized in that: The cabinet (1) is provided with a first observation port (21) and a second observation port (22) on the front side. A transparent front cabinet door (23) is provided on the front side of the second observation port (22). A rear cabinet door (24) is provided on the rear side of the cabinet (1). Casters (25) are provided at the bottom of the cabinet (1). Among them, the biological cryopreservation liquid dispensing pipeline (2) installed in the cabinet (1) can be seen through the first observation port (21) and the second observation port (22).

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