A cerebrospinal fluid preparation device for detecting Japanese encephalitis

Abstract

This utility model relates to the technical field of animal virus detection, and in particular to a cerebrospinal fluid preparation device for detecting Japanese encephalitis, which can centrifuge cerebrospinal fluid, making it easy to operate and improving the accuracy of detection; it includes a base plate; it also includes two upright plates, multiple centrifuge tubes, a centrifugation mechanism, a culture mechanism, a guide mechanism, and a support mechanism. The two upright plates are respectively fixedly installed at the top left and right ends of the base plate, the guide mechanism is installed at the top between the two upright plates, and the centrifugation mechanism is installed on the guide mechanism. The centrifugation mechanism includes an upper fixed plate, a connecting vertical plate, a lower fixed plate, a speed regulating motor, a gearbox, a drive wheel, and a guide wheel. Multiple centrifuge tubes are axially and evenly installed on the centrifugation mechanism, the culture mechanism is installed at the top center of the base plate, and the support mechanism is installed at the bottom four corners of the base plate.

Description

Technical Field

[0001] This utility model relates to the technical field of animal virus detection, and in particular to a cerebrospinal fluid preparation device for detecting Japanese encephalitis. Background Technology

[0002] Japanese encephalitis is an infectious disease caused by the Japanese encephalitis virus. When the meninges are irritated or inflamed, the white blood cell count in the cerebrospinal fluid (CSF) increases. To diagnose this disease, doctors usually extract a sample from the patient's CSF for testing. To ensure the accuracy of the test results, the CSF culture medium must be carefully prepared to avoid contamination and confusion. The prior art publication CN216499013U discloses a medical blood test reagent preparation device, including a box body. A top cover is movably connected to the upper surface of the box body via a rotating shaft. Fixed plates are fixedly installed on the upper ends of the left and right side walls inside the box body. Electric actuators are fixedly installed on the bottom surfaces of the two fixed plates. Movable plates are fixedly installed on the output ends of the two electric actuators. A sealing plate is fixedly installed on the bottom surface of the movable plate. The sealing plate is made of rubber, and its outer wall is tightly fitted to the inner wall of the box body. The two electric actuators drive the movable plate to move, causing the sealing plate to move along the inner wall of the box body, thereby adjusting the size of the stirring space to facilitate the preparation of different amounts of reagent. A motor drives the stirring rods to stir the reagents inside the box evenly. Multiple impellers rotate to further mix the reagent solution evenly, achieving a uniform mixing effect and improving the reagent preparation efficiency. However, after sampling, the cerebrospinal fluid needs to be quickly collected into a centrifuge tube, the supernatant removed, and then the cerebrospinal fluid sample placed in a culture medium containing nutrients. Pathogenic microorganisms are cultured and identified using the growth characteristics of bacteria, fungi, or viruses under suitable temperature and environmental conditions. The existing technology is not convenient for centrifuging cerebrospinal fluid, making it relatively inconvenient to use. Utility Model Content

[0003] To solve the above-mentioned technical problems, this utility model provides a cerebrospinal fluid preparation device for detecting Japanese encephalitis that can centrifuge cerebrospinal fluid, is easy to operate, and improves the accuracy of detection.

[0004] This utility model discloses a cerebrospinal fluid (CSF) preparation device for detecting Japanese encephalitis, comprising a base plate; two vertical plates, multiple centrifuge tubes, a centrifugation mechanism, a culture mechanism, a guiding mechanism, and a supporting mechanism. The two vertical plates are fixedly installed at the top left and right ends of the base plate, respectively. The guiding mechanism is installed at the top between the two vertical plates. The centrifugation mechanism is installed on the guiding mechanism, and the multiple centrifuge tubes are axially and evenly installed on the centrifugation mechanism. The culture mechanism is installed at the top center of the base plate, and the supporting mechanism is installed at the four bottom corners of the base plate. The supporting mechanism supports the device, facilitating the leveling of the base plate and improving stability during use. The centrifugation mechanism drives the centrifuge tubes to rotate within the guiding mechanism, centrifuging the CSF. The processed CSF is then input into the culture mechanism for culture, facilitating operation and improving the accuracy of detection.

[0005] Preferably, the guiding mechanism includes two mounting blocks, an annular track, and an annular slider. The two mounting blocks are respectively fixedly installed at opposite ends of the two upright plates, and the annular track is fixedly connected between the two mounting blocks. The annular track has a guide groove inside, and the annular slider is rotatably installed in the guide groove. When centrifuging cerebrospinal fluid, the annular slider is driven to rotate in the guide groove, which can guide and limit the movement, ensuring stability during centrifugation.

[0006] Preferably, the centrifuge mechanism includes an upper fixed plate, a connecting vertical plate, a lower fixed plate, a speed-regulating motor, a gearbox, a drive wheel, and a guide wheel. The outer wall of the upper fixed plate is fixedly connected to an annular slider. The connecting vertical plate is axially and uniformly fixedly installed at the bottom end of the upper fixed plate, and the lower fixed plate is fixedly installed at the bottom end of the connecting vertical plate. Multiple centrifuge tubes are axially and uniformly fixedly installed on the upper and lower fixed plates. A rotation groove is formed on the outer wall of the lower fixed plate. The speed-regulating motor and the gearbox are fixedly installed on the left side wall of the right vertical plate. The output end of the speed-regulating motor is connected to the input end of the gearbox, and the output end of the gearbox is connected to the drive wheel. The drive wheel is in close contact with the rotation groove of the lower fixed plate. The guide wheel is installed on the left vertical plate through a rotating frame, and the guide wheel is in close contact with the rotation groove of the lower fixed plate. When the speed-regulating motor is started, it drives the drive wheel to rotate through the gearbox. The drive wheel drives the lower fixed plate to rotate, thereby causing the multiple centrifuge tubes to rotate centrifugally. The guide wheel limits and guides the lower fixed plate to ensure stability during rotation.

[0007] Preferably, the system also includes two sliding rods, two sliding sleeves, a top cover, multiple telescopic rods, multiple pressure plates, and multiple springs. The two sliding rods are symmetrically installed on the top of the upper fixed plate, and the two sliding sleeves are slidably installed on the outer wall of the sliding rods. Fastening bolts are provided on the sliding sleeves. A top cover is fixedly installed between the two sliding sleeves, and a handle is provided at the top of the top cover. Multiple telescopic rods are axially and evenly installed at the bottom of the top cover, and pressure plates are fixedly installed at the bottom of the telescopic rods. The pressure plates are located directly above the centrifuge tubes, and multiple springs are respectively fitted onto the outer wall of the telescopic rods. During centrifugation, after the sealing plug is placed on top of the centrifuge tube, the sliding sleeves are fitted onto the sliding rods and slid downwards. The top cover drives the telescopic rods downwards, causing the pressure plates to contact the top of the sealing plug. Then, the telescopic rods and springs are further compressed downwards, ensuring that the pressure plates maintain tight contact with the sealing plug. Tightening the fastening bolts positions the sliding sleeves, preventing the sealing plug from falling off during centrifugation, which could lead to cerebrospinal fluid spillage, affecting subsequent testing and improving safety.

[0008] Preferably, the culture mechanism includes a heating base, an incubator, two first injection tubes, and a second injection tube. The heating base is fixedly installed at the top center of the base plate, and a placement groove is opened on the top of the heating base. The incubator is installed in the placement groove, and a magnetic stirrer is installed inside the incubator. The top left and right ends of the incubator are fixedly connected to the two first injection tubes, and the second injection tube is fixedly connected to the upper front end of the incubator. The centrifuged cerebrospinal fluid enters the incubator through the first injection tube, and then nutrients are added to the incubator through the second injection tube. The magnetic stirrer is started to mix and agitate the cerebrospinal fluid and nutrients. The heating base provides a suitable temperature for culturing the cerebrospinal fluid, which is convenient to operate and improves the accuracy of detection.

[0009] Preferably, the centrifuge tube also includes multiple graduation lines and multiple drain pipes. Graduation lines are provided on the side walls of the centrifuge tubes, and drain pipes are connected to the bottom of the centrifuge tubes. Valves are provided on the drain pipes, and the drain pipes at both ends are located above the first injection tube. The graduation lines facilitate the control of the amount of cerebrospinal fluid added to the centrifuge tubes, improving the detection accuracy. By opening the valves on the drain pipes, the processed cerebrospinal fluid can be directly added to the incubator through the first injection tube, improving the convenience of operation.

[0010] Preferably, the support mechanism includes multiple threaded sleeves, multiple threaded rods, multiple friction plates, and multiple adjusting nuts. The multiple threaded sleeves are respectively fixedly installed at the four corners of the bottom end of the base plate. The lower part of the threaded sleeve is screwed with a threaded rod, the bottom end of the threaded rod is connected to a friction plate, and the lower outer wall of the threaded rod is fixedly installed with an adjusting nut. In use, the friction plate increases the friction with the placement surface to prevent positional displacement during centrifugation. The adjusting nut drives the threaded rod to rotate, which can adjust the extension length of the threaded rod at the bottom of the threaded sleeve, making it easier to adjust the base plate to be level and improving the convenience of use.

[0011] Compared with the prior art, the beneficial effects of this utility model are as follows: the support mechanism supports the equipment, making it easy to adjust the base plate to be level and improving the stability during use; the centrifugation mechanism drives the centrifuge tube to rotate in the guide mechanism to centrifuge the cerebrospinal fluid, and then the processed cerebrospinal fluid is input into the culture mechanism for cerebrospinal fluid culture, which facilitates operation and improves the accuracy of detection. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a front view structural diagram of the present invention; Figure 3 This is a front cross-sectional structural diagram of the present invention; Figure 4 This is a schematic diagram of the right-side cross-sectional structure of this utility model; The following components are labeled in the attached diagram: 1. Base plate; 2. Vertical plate; 3. Centrifuge tube; 4. Mounting block; 5. Circular track; 6. Circular slider; 7. Upper fixing plate; 8. Connecting vertical plate; 9. Lower fixing plate; 10. Speed-regulating motor; 11. Gearbox; 12. Drive wheel; 13. Guide wheel; 14. Sliding rod; 15. Sliding sleeve; 16. Top cover; 17. Telescopic rod; 18. Pressure plate; 19. Spring; 20. Heating base; 21. Incubator; 22. First injection tube; 23. Second injection tube; 24. Magnetic stirrer; 25. Scale line; 26. Drain pipe; 27. Threaded sleeve; 28. Threaded rod; 29. ​​Friction plate; 30. Adjusting nut. Detailed Implementation

[0013] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. This utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to make the disclosure of this utility model more thorough and complete.

[0014] like Figure 1 , Figure 3 and Figure 4As shown, two upright plates 2 are fixedly installed at the top left and right ends of the base plate 1, respectively. Two mounting blocks 4 are fixedly installed at opposite ends of the two upright plates 2, and an annular track 5 is fixedly connected between the two mounting blocks 4. A guide groove is provided inside the annular track 5, and an annular slider 6 is rotatably installed in the guide groove. The outer wall of the upper fixed plate 7 is fixedly connected to the annular slider 6. A connecting vertical plate 8 is axially and evenly fixedly installed at the bottom end of the upper fixed plate 7, and a lower fixed plate 9 is fixedly installed at the bottom end of the connecting vertical plate 8. Multiple centrifuge tubes 3 are axially and evenly fixedly installed on the upper fixed plate 7 and the lower fixed plate 9. A rotating groove is provided on the outer wall of the lower fixed plate 9. A speed regulating motor 10 and a gearbox 11 are fixedly installed on the left side wall of the right upright plate 2. The output end of the speed regulating motor 10 is connected to the input end of the gearbox 11, and the output end of the gearbox 11 is connected to... A drive wheel 12 is connected to the bottom plate 1. The drive wheel 12 is in close contact with the rotation groove of the lower fixed plate 9. The guide wheel 13 is installed on the left vertical plate 2 through the rotating frame. The guide wheel 13 is in close contact with the rotation groove of the lower fixed plate 9. The heating base 20 is fixedly installed at the top center of the bottom plate 1. The top of the heating base 20 is provided with a placement groove. The incubator 21 is installed in the placement groove. The incubator 21 is equipped with a magnetic stirrer 24. The top left and right ends of the incubator 21 are fixedly connected to two first injection pipes 22. The second injection pipe 23 is fixedly connected to the upper front end of the incubator 21. Multiple threaded sleeves 27 are fixedly installed at the four corners of the bottom end of the bottom plate 1. The lower part of the threaded sleeve 27 is screwed with a threaded rod 28. The bottom end of the threaded rod 28 is connected to a friction plate 29. The lower outer wall of the threaded rod 28 is fixedly installed with an adjusting nut 30. During use, friction plate 29 increases friction with the placement surface to prevent positional shift during centrifugation. Adjusting nut 30 drives threaded rod 28 to rotate, adjusting the extension length of threaded rod 28 at the bottom of threaded sleeve 27, facilitating leveling of base plate 1 and improving ease of use. Starting speed-regulating motor 10 drives drive wheel 12 to rotate via gearbox 11, which in turn drives lower fixed plate 9 to rotate, causing multiple centrifuge tubes 3 to centrifuge. Guide wheel 13 limits and guides lower fixed plate 9 to ensure stability during rotation. Upper fixed plate 7 drives annular slider 6 to rotate within guide groove, providing guidance and limiting to ensure stability during centrifugation. Centrifuged cerebrospinal fluid enters incubator 21 through first injection tube 22, and then nutrients are added to incubator 21 through second injection tube 23. Magnetic stirrer 24 is started to mix and agitate cerebrospinal fluid and nutrients. Heating base 20 provides a suitable temperature for cerebrospinal fluid culture, facilitating operation and improving detection accuracy.

[0015] like Figure 2 and Figure 4As shown, two sliding rods 14 are symmetrically installed on the top of the upper fixed plate 7, two sliding sleeves 15 are slidably installed on the outer wall of the sliding rods 14, and fastening bolts are provided on the sliding sleeves 15. A top cover 16 is fixedly installed between the two sliding sleeves 15, and a handle is provided on the top of the top cover 16. Multiple telescopic rods 17 are axially and evenly installed at the bottom of the top cover 16. A pressure plate 18 is fixedly installed at the bottom of the telescopic rods 17. The pressure plate 18 is located directly above the centrifuge tube 3. Multiple springs 19 are respectively fitted on the outer wall of the telescopic rods 17. Scale lines 25 are provided on the side wall of the centrifuge tube 3. A drain pipe 26 is connected to the bottom of the centrifuge tube 3. A valve is provided on the drain pipe 26, and the drain pipes 26 at both ends are located above the first injection pipe 22. During centrifugation, after placing the sealing plug on top of the centrifuge tube 3, the sliding sleeve 15 is fitted onto the sliding rod 14 and slid downwards. The top cover 16 drives the telescopic rod 17 to move downwards, so that the pressure plate 18 contacts the top of the sealing plug. Then, the telescopic rod 17 and spring 19 are compressed downwards to keep the pressure plate 18 in close contact with the sealing plug. The fastening bolts are tightened to position the sliding sleeve 15, which can prevent the sealing plug from falling off during centrifugation, causing cerebrospinal fluid to spill out and affecting subsequent testing, thus improving safety. The scale line 25 makes it easy to control the amount of cerebrospinal fluid added to the centrifuge tube 3, improving the detection accuracy. By opening the valve on the drain pipe 26, the processed cerebrospinal fluid can be directly added to the incubator 21 through the first injection pipe 22, improving the convenience of operation.

[0016] like Figures 1 to 4 As shown, this utility model discloses a cerebrospinal fluid (CSF) preparation device for detecting Japanese encephalitis. During operation, adjusting the threaded rod 28 by adjusting the nut 30, and adjusting the extension length of the threaded rod 28 at the bottom of the threaded sleeve 27, leveles the base plate 1. The friction plate 29 increases the friction with the placement surface, quantitatively adding CSF into the centrifuge tube 3. The sealing plug is then placed on top of the centrifuge tube 3. The sliding sleeve 15 is then fitted onto the sliding rod 14 and slid downwards. The top cover 16 drives the telescopic rod 17 downwards, bringing the pressure plate 18 into contact with the top of the sealing plug. The telescopic rod 17 and spring 19 are further compressed downwards, ensuring tight contact between the pressure plate 18 and the sealing plug. Finally, the fastening bolts are tightened to position the sliding sleeve 15. The speed-regulating motor 10 is started, which drives the drive wheel 12 to rotate via the gearbox 11. The drive wheel 12 drives the lower fixed plate 9 to rotate, thereby causing multiple centrifuge tubes 3 to rotate centrifugally. The guide wheel 13 limits and guides the lower fixed plate 9. The upper fixed plate 7 drives the annular slider 6 to rotate in the guide groove, which can guide and limit the rotation, ensuring stability during centrifugation. The valve on the drain pipe 26 is opened, and the processed cerebrospinal fluid is directly added to the incubator 21 through the first injection pipe 22. Then, nutrients are added to the incubator 21 through the second injection pipe 23. The magnetic stirrer 24 is started to mix and agitate the cerebrospinal fluid and nutrients. The heating base 20 provides a suitable temperature for culturing the cerebrospinal fluid.

[0017] The speed-regulating motor 10, gearbox 11, heating base 20, and magnetic stirrer 24 of the cerebrospinal fluid preparation device for encephalitis B detection of this utility model are commercially available. Technical personnel in this industry only need to install and operate them according to the accompanying instruction manual, without requiring any creative work from those skilled in the art.

[0018] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A cerebrospinal fluid preparation device for detecting Japanese encephalitis, comprising a base plate (1); characterized in that, It also includes two upright plates (2), multiple centrifuge tubes (3), a centrifugation mechanism, a culture mechanism, a guide mechanism and a support mechanism. The two upright plates (2) are fixedly installed at the top left and right ends of the base plate (1), the guide mechanism is installed at the top between the two upright plates (2), the centrifugation mechanism is installed on the guide mechanism, the multiple centrifuge tubes (3) are axially and evenly installed on the centrifugation mechanism, the culture mechanism is installed at the top center of the base plate (1), and the support mechanism is installed at the bottom four corners of the base plate (1).

2. The cerebrospinal fluid preparation device for detecting Japanese encephalitis as described in claim 1, characterized in that, The guiding mechanism includes two mounting blocks (4), an annular track (5) and an annular slider (6). The two mounting blocks (4) are fixedly installed at opposite ends of the two upright plates (2). An annular track (5) is fixedly connected between the two mounting blocks (4). A guide groove is provided inside the annular track (5). The annular slider (6) is rotatably installed in the guide groove.

3. The cerebrospinal fluid preparation device for detecting Japanese encephalitis as described in claim 2, characterized in that, The centrifuge mechanism includes an upper fixed plate (7), a connecting vertical plate (8), a lower fixed plate (9), a speed-regulating motor (10), a gearbox (11), a drive wheel (12), and a guide wheel (13). The outer wall of the upper fixed plate (7) is fixedly connected to the annular slider (6). The connecting vertical plate (8) is axially and evenly fixedly installed at the bottom end of the upper fixed plate (7), and the lower fixed plate (9) is fixedly installed at the bottom end of the connecting vertical plate (8). Multiple centrifuge tubes (3) are axially and evenly fixedly installed on the upper fixed plate (7) and the lower fixed plate (9). A rotating groove is provided on the outer wall of the fixed plate (9). The speed regulating motor (10) and the gearbox (11) are fixedly installed on the left side wall of the right vertical plate (2). The output end of the speed regulating motor (10) is connected to the input end of the gearbox (11). The output end of the gearbox (11) is connected to the drive wheel (12). The drive wheel (12) is in close contact with the rotating groove of the lower fixed plate (9). The guide wheel (13) is installed on the left vertical plate (2) through the rotating frame. The guide wheel (13) is in close contact with the rotating groove of the lower fixed plate (9).

4. The cerebrospinal fluid preparation device for detecting Japanese encephalitis as described in claim 3, characterized in that, It also includes two sliding rods (14), two sliding sleeves (15), a top cover (16), multiple telescopic rods (17), multiple pressure plates (18), and multiple springs (19). The two sliding rods (14) are symmetrically installed on the top of the upper fixed plate (7). The two sliding sleeves (15) are slidably installed on the outer wall of the sliding rods (14). Fastening bolts are provided on the sliding sleeves (15). The top cover (16) is fixedly installed between the two sliding sleeves (15). The top of the top cover (16) is provided with a handle. Multiple telescopic rods (17) are axially and evenly installed at the bottom of the top cover (16). The bottom of the telescopic rods (17) is fixedly installed with pressure plates (18). The pressure plates (18) are located directly above the centrifuge tube (3). Multiple springs (19) are respectively fitted on the outer wall of the telescopic rods (17).

5. The cerebrospinal fluid preparation device for detecting Japanese encephalitis as described in claim 1, characterized in that, The culture mechanism includes a heating base (20), an incubator (21), two first injection tubes (22) and a second injection tube (23). The heating base (20) is fixedly installed at the top center of the base plate (1). A placement groove is opened on the top of the heating base (20). The incubator (21) is installed in the placement groove. A magnetic stirrer (24) is installed inside the incubator (21). Two first injection tubes (22) are fixedly connected to the left and right ends of the top of the incubator (21). The second injection tube (23) is fixedly connected to the upper front end of the incubator (21).

6. The cerebrospinal fluid preparation device for detecting Japanese encephalitis as described in claim 5, characterized in that, It also includes multiple scale lines (25) and multiple drain pipes (26). Scale lines (25) are provided on the side wall of the centrifuge tube (3). A drain pipe (26) is connected to the bottom end of the centrifuge tube (3). A valve is provided on the drain pipe (26), and the drain pipes (26) at both ends are located above the first injection pipe (22).

7. The cerebrospinal fluid preparation device for detecting Japanese encephalitis as described in claim 1, characterized in that, The support mechanism includes multiple threaded sleeves (27), multiple threaded rods (28), multiple friction plates (29), and multiple adjusting nuts (30). The multiple threaded sleeves (27) are fixedly installed at the four corners of the bottom of the base plate (1). The threaded rods (28) are screwed onto the lower part of the threaded sleeves (27). The friction plates (29) are connected to the bottom of the threaded rods (28). The adjusting nuts (30) are fixedly installed on the lower outer wall of the threaded rods (28).

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