An assisted reproductive culture dish

Abstract

This utility model discloses an assisted reproductive culture dish, relating to the field of assisted reproductive culture dish technology. It includes a culture dish with a separator fixedly connected to the center of its bottom, dividing the interior of the dish into two identical and symmetrical cavities. Each cavity initially contains a sperm cell reservoir. By controlling variables, it is possible to compare and observe reproductive fertilization under different conditions. When the sealing plate is pulled out, the liquid in both spaces of the culture dish flows through a leak, maintaining the same liquid environment. When the sealing plate is sealed within a rectangular frame, the liquid environment is separated, allowing the liquid environment in the two experimental spaces of the culture dish to be changed for experimentation. This control of variables facilitates comparison and observation of reproductive fertilization under different conditions. Furthermore, because the experiment is conducted within the same culture dish, variables can be better controlled, experimental errors reduced, and experimental accuracy improved.

Description

Technical Field

[0001] This utility model relates to the field of assisted reproductive culture dish technology, specifically an assisted reproductive culture dish. Background Technology

[0002] A petri dish is a laboratory vessel used for culturing microorganisms or cells. It consists of a flat, disc-shaped base and a lid, and is generally made of glass or plastic. Petri dishes are basically divided into two categories: plastic and glass. Glass petri dishes can be used for the culture of plant materials, microorganisms, and adherent cultures of animal cells.

[0003] Assisted reproductive culture dishes are used to simulate sperm and egg fertilization experiments to observe the fertilization of sperm and eggs under different variable conditions. In actual use, sperm, eggs, culture medium, etc. need to be placed in multiple culture dishes. In this case, due to the unavoidable errors in manual operation, or the different positions of the culture dishes in the incubator, the quantities that should be kept constant in different culture dishes will actually vary, thus affecting the accuracy of the experiment.

[0004] Therefore, an assisted reproductive culture dish is proposed. Utility Model Content

[0005] The purpose of this invention is to provide an assisted reproductive culture dish to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an assisted reproductive culture dish, comprising a culture dish with a separator fixedly connected to the center of the bottom of the culture dish, the separator dividing the culture dish into two identical and symmetrical cavities, each cavity containing a sperm groove and multiple egg grooves, and a channel connecting the multiple egg grooves and the sperm grooves in each cavity, the channel being formed inside the culture dish; a cover is detachably connected to the upper end of the culture dish; an observation port is provided on the cover, a cover plate is placed on the observation port, and a pull ring is fixedly connected to the center of the upper surface of the cover plate; Preferably, the outer surface of the petri dish is symmetrically fixed with buckles on both sides, and each buckle has a mark, with each mark corresponding to a space inside the petri dish.

[0007] Preferably, a downward inclined ring is fixedly connected to the middle of the egg groove, the downward inclined ring being annular and inclined downward, and an upward inclined ring is fixedly connected to the upper end face of the egg groove, the upward inclined ring being annular and inclined upward.

[0008] Preferably, the sperm groove is inverted conical, the egg groove is inverted conical, one end of the bottom surface of the channel is smoothly connected to the bottom of the sperm groove, and the other end of the bottom surface of the channel is connected to the upper end face of the lower inclined ring in the egg groove.

[0009] Preferably, the separator includes a rectangular frame, which is fixedly connected to the center of the bottom of the petri dish. The rectangular frame is densely perforated with holes, and a sealing plate extends through and is slidably connected to it. The sealing plate is T-shaped.

[0010] Preferably, the upper surface of the sealing plate is provided with a slot, the slot is polygonal, and a corresponding locking block is engaged in the slot, the upper end of the locking block is fixedly connected to the center of the bottom of the cover plate.

[0011] Compared with the prior art, the beneficial effects of this utility model are: The snap-on design of this invention facilitates the handling of the culture dish, and the markings on the snap-on plate help identify the two reproductive fertilization experiments, preventing confusion. The separator design allows for simultaneous two reproductive fertilization experiments within the culture dish. By controlling variables, it is possible to compare and observe reproductive fertilization under different conditions, facilitating experimental research. The rectangular frame, perforation, and sealing plate design within the separator also aim to control variables. When the sealing plate is pulled out, the liquid in both spaces of the culture dish flows through the perforation, maintaining the same liquid environment. When the sealing plate is sealed within the rectangular frame, the liquid environment is separated, allowing for changes in the liquid environment within the two experimental spaces of the culture dish to control variables. By controlling variables, it is possible to compare and observe reproductive fertilization under different conditions. Furthermore, since the experiment is conducted within the same culture dish, variables can be better controlled, experimental errors can be reduced, and the accuracy of the experiment can be improved. Attached Figure Description

[0012] Figure 1 This is an overall structural diagram of the present invention; Figure 2 This is an overall sectional view of the present invention; Figure 3 This is a partial sectional view of the present invention; Figure 4 This is a cross-sectional view of the present invention; Figure 5 This is a side sectional view of the present invention.

[0013] In the diagram: 1. Petri dish; 2. Separator; 3. Sperm trough; 4. Oocyte trough; 5. Channel; 6. Cover; 7. Observation port; 8. Cover plate; 9. Pull ring; 10. Buckle plate; 11. Marker; 12. Lower sloping ring; 13. Upper sloping ring; 14. Rectangular frame; 15. Leakage hole; 16. Sealing plate; 17. Slot; 18. Block. Detailed Implementation

[0014] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0015] Please see Figures 1 to 5 This utility model provides a technical solution: An assisted reproductive culture dish includes a culture dish 1. A separator 2 is fixedly connected to the center of the bottom of the culture dish 1, dividing the interior of the culture dish 1 into two identical and symmetrical cavities. Each cavity contains a sperm groove 3 and multiple egg grooves 4. A channel 5 connects the multiple egg grooves 4 in each cavity to the sperm grooves 3 in that cavity. The channel 5 is located inside the culture dish 1. A cover 6 is detachably connected to the upper end of the culture dish 1. The cover 6 has an observation port 7, and a cover plate 8 is placed on the observation port 7. A pull ring 9 is fixedly connected to the center of the upper end face of the cover plate 8.

[0016] As an embodiment of this utility model, as shown in the figure, the outer surfaces of the petri dish 1 are symmetrically fixed with buckle plates 10, and each buckle plate 10 is provided with a mark 11, and each mark 11 corresponds to a space inside the petri dish 1.

[0017] During operation, cover the petri dish 1 with the lid 6 and place it in a 37°C, 6% CO2 incubator overnight to equilibrate. When observing, remove the petri dish 1 from the incubator and remove the lid 8 through the pull ring 9. Observe the fertilization status in the petri dish 1 through a microscope. The design of the pull ring 9, the lid 8 and the observation port 7 is to facilitate the observation of the fertilization status in the petri dish 1 through a microscope. The design of the cover plate 10 is to facilitate the handling of the culture dish 1, and the marking 11 on the cover plate 10 helps us to identify the two reproductive fertilization experiments and prevent confusion.

[0018] As an embodiment of this utility model, as shown in the figure, a downward inclined ring 12 is fixedly connected to the middle of the egg groove 4. The downward inclined ring 12 is circumferentially inclined downwards. An upward inclined ring 13 is fixedly connected to the upper end face of the egg groove 4. The upward inclined ring 13 is circumferentially inclined upwards.

[0019] The sperm groove 3 is an inverted cone shape, the egg groove 4 is an inverted cone shape, one end of the bottom of the inner surface of the channel 5 is smoothly connected to the bottom of the sperm groove 3, and the other end of the bottom of the inner surface of the channel 5 is connected to the upper end face of the lower inclined ring 12 in the egg groove 4.

[0020] During operation, the design of the downward oblique ring 12 inside the egg groove 4 allows sperm to enter the egg groove 4 and fall to the bottom of the egg groove 4, where they are blocked by the downward oblique ring 12 and return to the sperm groove 3 through the channel 5, thus preventing sperm backflow. The design of the upward oblique ring inside the egg groove 4 is to stabilize the liquid environment inside the egg groove 4, so as to provide a good fertilization environment.

[0021] As an embodiment of this utility model, as shown in the figure, the separator 2 includes a rectangular frame 14, which is fixedly connected to the center of the bottom of the petri dish 1. The rectangular frame 14 is densely provided with perforations 15. A sealing plate 16 extends through and is slidably connected to the rectangular frame 14. The sealing plate 16 is T-shaped. A slot 17 is provided on the upper surface of the sealing plate 16. The slot 17 is polygonal, and a corresponding locking block 18 is fitted into the slot 17. The upper end of the locking block 18 is fixedly connected to the center of the bottom of the cover plate 8.

[0022] During operation, the design of the separator 2 allows the petri dish 1 to conduct two reproductive fertilization experiments simultaneously. By controlling variables, it is possible to compare and observe reproductive fertilization under different conditions, which is convenient for experimental research. The design of the rectangular frame 14, the leakage hole 15, and the sealing plate 16 in the separator 2 is also for controlling variables. That is, when the sealing plate 16 is pulled out, the liquid in the two spaces of the petri dish 1 flows through the leakage hole 15, and its liquid environment is the same. When the sealing plate 16 is sealed inside the rectangular frame 14, its liquid environment is separated. The liquid environment in the two experimental spaces of the petri dish 1 can be changed to carry out the experiment, thereby controlling variables. The design of the slot 17 and the block 18 is to press the sealing plate 16 below when it is necessary to separate the liquid environment, so as to prevent the liquid in the petri dish 1 from leaking from the contact point between the sealing plate 16 and the bottom of the petri dish 1, causing experimental errors.

[0023] Working principle: A small drop of fertilization culture medium, balanced at 37°C and 6% CO2 concentration, is placed in each sperm tank 3 and egg tank 4. Then, the fertilization culture medium in each egg tank 4 and sperm tank 3 in each culture dish 1 is connected through channel 5 to create an environment for contact between eggs and sperm. Cover the culture dish 1 with 3-3.5 mL of paraffin oil, and place one or two eggs in each egg groove 4. Then, place sperm in each sperm groove 3. The amount of sperm added in each sperm groove 3 is calculated based on the sperm density, and the volume is generally 10-20 μL. The cover plate 10 is designed to facilitate the handling of the culture dish 1, and the marking 11 on the cover plate 10 helps us identify the two reproductive fertilization experiments and prevent confusion. The design of the separator 2 allows two reproductive fertilization experiments to be conducted simultaneously in this culture dish 1. By controlling variables, it is possible to compare and observe the reproductive fertilization situation under different conditions. Furthermore, since the experiment is conducted in the same culture dish 1, variables can be better controlled, reducing experimental errors. To ensure experimental accuracy, the design of the rectangular frame 14, the leak 15, and the sealing plate 16 in the separator 2 is also for controlling variables. When the sealing plate 16 is pulled out, the liquid in the two spaces of the petri dish 1 flows through the leak 15, and the liquid environment is the same. When the sealing plate 16 is sealed inside the rectangular frame 14, the liquid environment is separated. The liquid environment in the two experimental spaces of the petri dish 1 can be changed to conduct experiments, thereby controlling variables. The design of the slot 17 and the locking block 18 is to press the sealing plate 16 below when it is necessary to separate the liquid environment, so as to prevent the liquid in the petri dish 1 from leaking from the contact point between the sealing plate 16 and the bottom of the petri dish 1, causing experimental errors. The fertilization process is as follows: sperm in sperm groove 3 enters egg groove 4 through channel 5 and fertilizes the egg. The downward oblique ring 12 in egg groove 4 is designed to allow sperm to fall to the bottom of egg groove 4 after entering egg groove 4 and be obstructed by the downward oblique ring 12, so that they return to sperm groove 3 through channel 5, thus preventing sperm backflow. The upward oblique ring in egg groove 4 is designed to stabilize the liquid environment in egg groove 4 to provide a good fertilization environment. Then, cover the petri dish 1 with the lid 6 and place it in a 37°C, 6% CO2 incubator to equilibrate overnight. When observing, remove the petri dish 1 from the incubator and then remove the lid 8 through the pull ring 9. Observe the fertilization status in the petri dish 1 through a microscope. The design of the pull ring 9, the lid 8 and the observation port 7 is to facilitate the observation of the fertilization status in the petri dish 1 through a microscope.

[0024] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An assisted reproduction culture dish comprising a culture dish (1) characterised in that: A separator (2) is fixedly connected to the center of the bottom of the culture dish (1). The separator (2) divides the culture dish (1) into two identical and symmetrical cavities. Each cavity has a sperm groove (3) and multiple egg grooves (4). A channel (5) connects the multiple egg grooves (4) in each cavity to the sperm grooves (3) in that cavity. The channel (5) is located inside the culture dish (1). A cover (6) is detachably connected to the upper end of the culture dish (1). An observation port (7) is provided on the cover (6). A cover plate (8) is placed on the observation port (7). A pull ring (9) is fixedly connected to the center of the upper surface of the cover plate (8).

2. An assisted reproduction culture dish according to claim 1, wherein: The outer surface of the petri dish (1) is symmetrically fixed with buckle plates (10), and each buckle plate (10) is provided with a mark (11), and each mark (11) corresponds to a space inside a petri dish (1).

3. An assisted reproduction culture dish according to claim 2, wherein: A downward inclined ring (12) is fixedly connected to the middle of the egg groove (4). The downward inclined ring (12) is circumferentially inclined downward. An upward inclined ring (13) is fixedly connected to the upper end face of the egg groove (4). The upward inclined ring (13) is circumferentially inclined upward.

4. An assisted reproduction culture dish according to claim 3, wherein: The sperm groove (3) is an inverted cone shape, the egg groove (4) is an inverted cone shape, one end of the bottom of the inner surface of the channel (5) is smoothly connected to the bottom of the sperm groove (3), and the other end of the bottom of the inner surface of the channel (5) is connected to the upper end of the lower inclined ring (12) in the egg groove (4).

5. The assisted reproductive culture dish of claim 1, wherein: The separator (2) includes a rectangular frame (14), which is fixedly connected to the center of the bottom of the petri dish (1). Drain holes (15) are densely opened on the rectangular frame (14). A sealing plate (16) extends through and is slidably connected to the rectangular frame (14). The sealing plate (16) is T-shaped.

6. An assisted reproduction culture dish according to claim 5, wherein: The upper surface of the sealing plate (16) is provided with a slot (17), the slot (17) is polygonal, and a corresponding locking block (18) is fitted in the slot (17), the upper end of the locking block (18) is fixedly connected to the center of the bottom of the cover plate (8).

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