A detachable microfluidic cell chip

Abstract

This utility model discloses a detachable microfluidic cell chip, characterized by comprising a base plate, a transparent sheet, a separator, and a cover plate. The base plate has a mounting chamber at its top, one side of which communicates with the side wall of the base plate. One end of the transparent sheet is detachably mounted within the mounting chamber. The separator has at least one separator chamber, both ends of which penetrate the top and bottom surfaces of the separator. The bottom of the separator is detachably disposed within the mounting chamber, and the separator is positioned directly opposite the transparent sheet. The cover plate is detachably mounted on the base plate above the separator, pressing the bottom surface of the separator against the top surface of the transparent sheet. The top surface of the cover plate also has a flow channel opening facing the separator chamber. This utility model improves ease of use and reduces costs.

Description

Technical Field

[0001] This utility model relates to the field of cell culture and detection, and in particular to a detachable microfluidic cell chip. Background Technology

[0002] Microfluidics technology has seen accelerated applications in cell culture and detection in recent years. Microfluidics refers to the science and technology involved in processing or manipulating tiny fluid systems using microchannels (tens to hundreds of micrometers in size). It is an emerging interdisciplinary field involving chemistry, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Its devices are characterized by miniaturization and integration, and common microfluidic devices are also known as microfluidic chips.

[0003] In existing technologies, microfluidic chips are either non-removable or inconvenient to disassemble, or a single microfluidic chip can only culture and detect the same type of cells. If different cells need to be cultured and detected, or if the same type of cells needs to be cultured and detected in different ways, a separate microfluidic chip is required for each type of cell culture and detection, or for each method of cell culture and detection, resulting in relatively high costs. Moreover, most chips on the market are for single use only and cannot be sterilized and reused, which is also costly. In addition, when placing the cells in the microfluidic chip under a microscope for observation, the cells need to be transferred to a glass slide before being placed under the microscope. This process requires a transfer step and may pose a risk of contamination. Summary of the Invention

[0004] The purpose of this invention is to provide a detachable microfluidic cell chip, which improves the convenience of cell culture and detection and reduces costs.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is: a detachable microfluidic cell chip, including a base plate, a transparent sheet, a separator, and a cover plate. The top of the base plate is provided with an installation chamber, one side of which is connected to the side wall of the base plate. One end of the transparent sheet is detachably installed in the installation chamber, and the other end of the transparent sheet protrudes from one side of the installation chamber and is disposed outside the base plate. The separating component is provided with at least one separating chamber, the two ends of the separating chamber penetrate through the top and bottom surfaces of the separating component, the bottom of the separating component is detachably disposed in the mounting chamber, and the separating chamber is disposed directly opposite the transparent sheet; The cover plate is detachably installed on the base plate above the partition component, and the cover plate presses the bottom surface of the partition component against the top surface of the transparent sheet. The top surface of the cover plate is also provided with a flow channel opening facing the partition chamber.

[0006] In the above technical solution, the top surface of the separating component abuts against the bottom surface of the cover plate, and the bottom surface of the separating component abuts against the top surface of the transparent sheet; And / or, the separator is made of PDMS, silicone or TPU.

[0007] In the above technical solution, the cover plate is detachably connected to the base plate via a connector.

[0008] In the above technical solution, the connecting component is a bolt, and at least two screw holes are provided on the top surface of the base plate outside the mounting cavity. At least two connecting holes are provided on the cover plate, which are respectively facing the screw holes. The bolt passes through the connecting hole and is screwed into the corresponding screw hole.

[0009] In the above technical solution, the separating component is an elastic seal, and the thickness of the elastic seal is greater than the depth of the mounting cavity between the top surface of the transparent sheet and the top surface of the base plate.

[0010] In the above technical solution, each of the partition chambers is connected to at least one of the flow channels.

[0011] In the above technical solution, each of the partition chambers is connected to at least one of the flow channels, and the two ends of the flow channels are connected to the top and bottom surfaces of the cover plate, respectively. Furthermore, each of the flow channels is provided with an isolation barrier.

[0012] In the above technical solution, the side wall of the partition component is provided with multiple through holes, and each partition chamber communicates with at least one of the through holes; The side wall of the base plate is provided with external through holes that correspond one-to-one with the through holes, and each external through hole is connected to one of the through holes.

[0013] In the above technical solution, the isolation barrier is a hydrogel or a porous membrane.

[0014] In the above technical solution, the bottom surface of the base plate is provided with a through groove communicating with the mounting chamber, and the size of the through groove is smaller than the size of the mounting chamber; And / or, the outer edge of the bottom surface of the transparent sheet abuts against the bottom surface of the mounting chamber next to the through groove.

[0015] Due to the application of the above technical solution, this utility model has the following advantages compared with the prior art: 1. The microfluidic chip in this utility model is detachable and uses multiple components to form independent culture and detection chambers by using transparent sheets and separators. The same type of cells or different cells can be cultured and detected on a single microfluidic chip, which effectively improves the convenience of cell culture and detection and also reduces costs. 2. The microfluidic chip in this invention can be quickly disassembled, enabling modular manufacturing, low cost, and can be reused multiple times, reducing usage costs; 3. This utility model reduces the number of transfer steps and lowers the risk of contamination during the transfer process. Attached Figure Description

[0016] Figure 1 This is a structural schematic diagram of Embodiment 1 of this utility model (the bolts are not shown when the cover plate and the bottom plate are separated). Figure 2 yes Figure 1 Exploded view; Figure 3 This is a structural schematic diagram of Embodiment 2 of this utility model (the bolts are not shown when the cover plate and the bottom plate are separated). Figure 4 yes Figure 3 Exploded view.

[0017] The components include: 1. base plate; 11. mounting chamber; 12. through groove; 13. screw hole; 2. Transparent film; 3. Separating components; 31. Separating chambers; 4. Cover plate; 41. Flow channel opening; 42. Connecting hole; 5. Base plate; 51. Mounting chamber; 52. Through groove; 53. Screw hole; 54. External through hole; 6. Transparent film; 7. Separating component; 71. Separating chamber; 72. Through hole; 8. Cover plate; 81. Flow channel opening; 82. Connection hole; 83. Isolation barrier. Detailed Implementation

[0018] The present invention will be further described below with reference to the accompanying drawings and embodiments: Example 1: See Figure 1 , 2 As shown, a detachable microfluidic cell chip includes a base plate 1, a transparent sheet 2, a separator 3, and a cover plate 4. The top of the base plate 1 is provided with an installation chamber 11, one side of which is connected to the side wall of the base plate 1. One end of the transparent sheet 2 is detachably installed in the installation chamber 11, and the other end of the transparent sheet 2 extends out from one side of the installation chamber 11 and is disposed outside the base plate 1. The separating component 3 is provided with at least one separating chamber 31. Both ends of the separating chamber 31 penetrate the top and bottom surfaces of the separating component 3. The bottom of the separating component 3 is detachably disposed in the mounting chamber 11, and the separating chamber 31 is disposed directly opposite the transparent sheet 2. The cover plate 4 is detachably installed on the base plate 1 above the partition component 3, and the cover plate presses the bottom surface of the partition component 3 against the top surface of the transparent sheet 2. The top surface of the cover plate 4 is also provided with a flow channel 41 facing the partition chamber 3. The cover plate is detachably connected to the base plate via a connector.

[0019] In this invention, there is at least one partition chamber. In this embodiment, four partition chambers are used as an example (the number can be more or less, depending on the actual situation). The right side of the installation chamber is connected to the right side of the base plate. The transparent sheet is one of transparent glass, transparent glass slide, transparent PMMA sheet or transparent PS plastic sheet. In this embodiment, the transparent sheet is a glass slide, on which biomaterial (containing living cells) is printed. The biomaterial is printed on the transparent sheet by 3D printing. Of course, other methods can also be used to place the biomaterial on the top surface of the transparent sheet. The biomaterial is spaced apart, and each partition chamber has one point of biomaterial. After the biomaterial sites on the transparent sheet are set, the operator grasps or clamps the right end of the transparent sheet (the right end of the transparent sheet is outside the right side of the installation chamber, while the biomaterial sites are inside the installation chamber) and places the transparent sheet on the bottom surface of the installation chamber of the base plate. Then, the separator is placed into the installation chamber above the transparent sheet. The transparent sheet has four biomaterial sites, each located in a separate separator chamber, thus individually separating each biomaterial site. The cover plate is then pressed onto the separator and connected to the base plate to achieve positioning, ensuring that each separator chamber is an independent chamber.

[0020] See Figure 1 , 2 As shown, the top surface of the separating component 3 abuts against the bottom surface of the cover plate 4, and the bottom surface of the separating component 3 abuts against the top surface of the transparent sheet 2.

[0021] The separating component is an elastic seal, and the thickness of the elastic seal is greater than the depth of the mounting chamber between the top surface of the transparent sheet and the top surface of the base plate. The separating component is made of PDMS, silicone, or TPU. Therefore, the separating component is an elastic sealing gasket. In the initial state, with the bottom of the separating component against the top of the transparent sheet, the top surface of the separating component is above the top of the mounting chamber and above the top surface of the base plate. When the cover plate is installed, the bottom surface of the cover plate presses against the top surface of the separating component. Then, the base plate and the cover plate are connected by a connector. When the connector connects the base plate and the cover plate, it will press down on the cover plate, and through the downward pressure of the cover plate, the separating component is pressed tightly onto the transparent sheet, thereby ensuring that the bottom of the separating chamber does not communicate with adjacent separating chambers, so that the separating chambers form independent chambers.

[0022] See Figure 1 , 2 As shown, the bottom surface of the base plate 1 is provided with a through groove 12 that communicates with the mounting chamber 11, and the size of the through groove 12 is smaller than the size of the mounting chamber 11; The outer edge of the bottom surface of the transparent sheet 2 abuts against the bottom surface of the mounting chamber 11 on the side of the through groove 12.

[0023] In this method, the base plate has a hollow structure, which makes it easy to observe the transparent sheet under a microscope (the transparent sheet is not removed from the base plate and is placed directly on the microscope for observation).

[0024] The connecting component is a bolt (not shown in the bolt diagram). At least two screw holes 13 are provided on the top surface of the base plate 1 outside the mounting chamber 11. At least two connecting holes 42 are provided on the cover plate, each corresponding to one of the screw holes 13. The bolt passes through the connecting hole 42 and is screwed into the corresponding screw hole 13. In this embodiment, there are six screw holes and six connecting holes. Three screw holes are located on the top surface of the base plate on the front side of the mounting chamber, and the other three screw holes are located on the top surface of the base plate on the rear side of the mounting chamber. There are also six connecting holes, each corresponding to one screw hole.

[0025] Of course, the connector can also be of other structures, as long as it can lock the cover plate and the base plate together.

[0026] See Figure 1 , 2 As shown, each of the partition chambers 31 is connected to at least one of the flow channels 41.

[0027] In this embodiment, each compartment is connected to two flow channels, which are the inlet and outlet, respectively. A peristaltic pump or other power unit can be used to deliver the culture medium / detection solution (external liquid, the liquid medium required for the culture, detection, or other purposes of biological materials) from the inlet into the corresponding compartment and then out of the outlet for the culture or detection of biological materials (cells). Only one peristaltic pump or other power unit can be used, connected to all flow channels via piping, simultaneously delivering the culture medium / detection solution to all compartments (in this embodiment, the flow channel size is set relatively small for connection to the piping). Alternatively, one peristaltic pump or power unit can be used per compartment to deliver the culture medium / detection solution required for the corresponding biological material, ensuring dynamic liquid flow and contact with the biological material.

[0028] In this embodiment, the cover plate is made of PMMA, aluminum alloy, stainless steel, polytetrafluoroethylene, or PS material. If the cover plate is not made of metal, it can be made of a transparent material to facilitate observation of the cultivation / detection of the internal biological material.

[0029] In this structure, multiple chambers can be formed using the same microfluidic cell chip to culture / detect the same or different biological materials, enabling high-throughput culture / detection and reducing costs. Moreover, depending on the actual situation, different numbers of partition components can be selected to use.

[0030] During disassembly, the cover plate and base plate are separated, and then the separating component is removed from the transparent sheet. The transparent sheet can then be removed from the base plate. Furthermore, it features modular production, high throughput, and can be reused through sterilization, resulting in low cost.

[0031] Meanwhile, before or after removing the slide from the substrate, the image can be directly placed under a high-definition microscope to read the results (the slide can be placed directly on the microscope without being removed from the substrate, or it can be removed from the substrate and placed directly on the microscope), which is more convenient and reduces the step of manually transferring cells to a slide for observation, thus reducing the potential risks of cell contamination during manual operation.

[0032] Example 2: See Figure 3 , 4 As shown, a detachable microfluidic cell chip includes a base plate 5, a transparent sheet 6, a separator 7, and a cover plate 8. The top of the base plate 5 is provided with an installation chamber 51. One side of the installation chamber 51 is connected to the side wall of the base plate 5. One end of the transparent sheet 6 is detachably installed in the installation chamber 51, and the other end of the transparent sheet 6 protrudes from one side of the installation chamber 51 and is disposed outside the base plate 5. The separating component 7 is provided with at least one separating chamber 71. Both ends of the separating chamber 71 penetrate the top and bottom surfaces of the separating component 7. The bottom of the separating component 7 is detachably disposed in the mounting chamber 51, and the separating chamber 71 is disposed directly opposite the transparent sheet 6. The cover plate 8 is detachably mounted on the base plate 5 above the partition component 7, and the cover plate presses the bottom surface of the partition component 7 against the top surface of the transparent sheet 6. The top surface of the cover plate 8 is also provided with a flow channel 81 facing the partition chamber 71. The cover plate is detachably connected to the base plate via a connector.

[0033] In this invention, there is at least one partition chamber. In this embodiment, four partition chambers are used as an example. The right side of the installation chamber is connected to the right side of the base plate. The transparent sheet is one of a transparent glass plate, a transparent PMMA sheet, or a transparent PS plastic sheet. In this embodiment, the transparent sheet is a glass slide on which biomaterial (containing living cells) is printed. The biomaterial is printed on the transparent sheet by 3D printing. Of course, other methods can also be used to place the biomaterial on the top surface of the transparent sheet. The biomaterial is spaced apart, and each partition chamber is provided with one point of biomaterial. After the biomaterial sites are set on the transparent sheet, the operator grasps or clamps the right end of the transparent sheet (the right side of the transparent sheet will be outside the right side of the mounting chamber's bottom plate, while the biomaterial sites will be inside the mounting chamber), places the transparent sheet on the bottom surface of the mounting chamber on the bottom plate, and then inserts the partition component into the mounting chamber above the transparent sheet. The transparent sheet has four biomaterial sites, each located in a separate partition chamber, thus individually separating each biomaterial site. The cover plate is then pressed onto the partition component and connected to the bottom plate to achieve positioning, ensuring that each partition chamber is an independent chamber.

[0034] See Figure 3 , 4 As shown, the top surface of the separating component 7 abuts against the bottom surface of the cover plate 8, and the bottom surface of the separating component 7 abuts against the top surface of the transparent sheet 6.

[0035] The separating component is an elastic seal, and the thickness of the elastic seal is greater than the depth of the mounting chamber between the top surface of the transparent sheet and the top surface of the base plate. The separating component is made of PDMS, silicone, or TPU. Therefore, the separating component is an elastic sealing gasket. In the initial state, with the bottom of the separating component against the top of the transparent sheet, the top surface of the separating component is above the top of the mounting chamber and above the top surface of the base plate. When the cover plate is installed, the bottom surface of the cover plate presses against the top surface of the separating component. Then, the base plate and the cover plate are connected by a connector. When the connector connects the base plate and the cover plate, it will press down on the cover plate, and through the downward pressure of the cover plate, the separating component is pressed tightly onto the transparent sheet, thereby ensuring that the bottom of the separating chamber does not communicate with adjacent separating chambers, so that the separating chambers form independent chambers.

[0036] See Figure 3 , 4 As shown, the bottom surface of the base plate 5 is provided with a through groove 52 that communicates with the mounting chamber 51, and the size of the through groove 52 is smaller than the size of the mounting chamber 51; The outer edge of the bottom surface of the transparent sheet 6 abuts against the bottom surface of the mounting chamber 51 next to the through groove 52.

[0037] In this method, the base plate has a hollow structure, which facilitates microscopic observation.

[0038] The connecting component is a bolt (not shown in the figure). At least two screw holes 53 are provided on the top surface of the base plate 5 outside the mounting chamber 51. At least two connecting holes 82 are provided on the cover plate 8, each corresponding to one of the screw holes 53. The bolt passes through the connecting hole 82 and is screwed into the corresponding screw hole 53. In this embodiment, there are six screw holes and six connecting holes. Three screw holes are located on the top surface of the base plate on the front side of the mounting chamber, and the other three screw holes are located on the top surface of the base plate on the rear side of the mounting chamber. There are also six connecting holes, each corresponding to one screw hole.

[0039] Of course, the connector can also be of other structures, as long as it can lock the cover plate and the base plate together.

[0040] In this embodiment, the cover plate is made of PMMA, aluminum alloy, stainless steel, polytetrafluoroethylene, or PS material. If the cover plate is not made of metal, it can be made of a transparent material to facilitate observation of the cultivation / detection of the internal biological material.

[0041] See Figure 3 , 4 As shown, the side wall of the partition component 7 is provided with a plurality of through holes 72, and each partition chamber 71 communicates with at least one of the through holes 72; The side wall of the base plate 5 is provided with external through holes 54 corresponding to the through holes 72. One end of the external through hole 54 communicates with the outer side wall of the base plate 5, and the other end communicates with the mounting chamber 51. Each external through hole 54 communicates with one of the through holes 72. In this embodiment, the right side of the mounting chamber communicates with the right side of the base plate, and the through holes are set on the front side wall of the partition component, while the external through holes are set on the front side wall of the base plate.

[0042] In this embodiment, each partition chamber is provided with a through hole that communicates with the outer wall of the partition component, that is, the partition component is provided with 4 through holes, and the side wall of the bottom plate is provided with 4 external through holes, each external through hole being directly opposite a through hole.

[0043] In this embodiment, under normal conditions, the width of the partition component matches the width of the mounting chamber. After the partition component is placed into the mounting chamber, its sidewall abuts against the sidewall of the mounting chamber. Because the partition component is made of elastic material, after being compressed and deformed by the cover plate, its sidewall will tightly adhere to the sidewall of the mounting chamber, thus ensuring that the through hole can only communicate with the external through hole. To facilitate the cultivation / detection of biological materials within the partition chamber and to facilitate the delivery of culture medium / detection solution into the partition chamber for utilization by the biological materials, the partition chamber and the external bottom plate are connected by the through hole. A peristaltic pump or other power component can be used to deliver culture medium / detection solution into and remove it from the partition chamber for replacement via a pipeline connected to the corresponding external through hole. Alternatively, each compartment can have two through holes, and the side wall of the bottom plate can have external through holes facing the corresponding through holes. One through hole serves as the inlet for the culture medium / detection solution, and the other through hole serves as the outlet for the culture medium / detection solution, thus enabling the culture medium / detection solution to flow into contact with the biological material.

[0044] In cell-chip microarrays, there is a type of model that requires the establishment of a barrier. This type of model can form a barrier (such as the blood-brain barrier), allowing the drug to be detected to penetrate the barrier and act on the cells within the chamber, thereby increasing the accuracy of the detection. Therefore, to target the aforementioned cells, see [link to relevant documentation]. Figure 3 , 4 As shown, each of the partition chambers 71 is connected to one of the flow channels 81.

[0045] The two ends of the flow channel 81 are connected to the top and bottom surfaces of the cover plate 8, respectively, and each flow channel 81 is also provided with an isolation barrier 83. The isolation barrier is a hydrogel or a porous membrane. In this embodiment, there are 4 partitioned chambers, and the cover plate is provided with 4 flow channels.

[0046] By establishing a barrier to enable the co-culture of different cells, the interaction between different cells can be studied, which has high scientific research value and detection value (in this embodiment, the flow channel opening size is set to be larger than that in Example 1, which facilitates the arrangement of the isolation barrier and thus increases the contact area with the drug).

[0047] One method involves using a hydrogel to create the isolation barrier. The hydrogel is poured into the flow channel and allowed to solidify, forming a barrier that mimics the extracellular matrix. Alternatively, a porous membrane can be directly attached to the flow channel to achieve the same isolation barrier. Both hydrogels and porous membranes allow some drug to permeate. During use, the pores allow culture / detection solutions to be introduced into the compartment, providing the biological material (cells) with the appropriate culture / detection substances. The cover plate contains the drug to be detected, or other substances that can penetrate the isolation barrier to enter the compartment and contact the biological material, thereby improving detection accuracy.

[0048] In this structure, hydrogels or different porous membranes can be selected to establish isolation barriers for different cell models, depending on the actual situation. It is convenient and easy to use, highly practical, and has a wide range of applications.

[0049] Similarly, the chip can be directly placed under a microscope for observation without disassembly. Disassembly involves separating the cover plate from the base plate, then removing the separator from the transparent slide. The transparent slide can then be removed from the base plate for preservation and encapsulation of the cells. It features modular production, high throughput, and reusability through sterilization (the isolation barrier can be manually removed and sterilized, and then replaced according to different cells or specific conditions), resulting in low cost.

[0050] The method of removing the transparent slide from the base plate or not and placing it directly under a high-definition microscope for imaging and reading results is more convenient. It reduces the need to manually transfer cells to a glass slide for observation, thus reducing the risk of cell contamination during manual operation.

[0051] In this structure, multiple chambers can be formed using the same microfluidic cell chip to culture / detect the same or different biological materials, which will reduce costs. Moreover, depending on the actual situation, different numbers of partition components can be selected to separate the chambers.

[0052] In the description of this utility model, it should be understood that the terms "upper," "lower," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. In the description of the invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0053] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. For instance, the two components can be mechanically connected by contact or abutting; they can also be directly hooked or connected by an intermediate medium; or they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

Claims

1. A detachable microfluidic cell chip, characterized in that: The device includes a base plate, a transparent sheet, a partition component, and a cover plate. The top of the base plate is provided with an installation chamber. One side of the installation chamber is connected to the side wall of the base plate. One end of the transparent sheet is detachably installed in the installation chamber, and the other end of the transparent sheet protrudes from one side of the installation chamber and is disposed outside the base plate. The separating component is provided with at least one separating chamber, the two ends of the separating chamber penetrate through the top and bottom surfaces of the separating component, the bottom of the separating component is detachably disposed in the mounting chamber, and the separating chamber is disposed directly opposite the transparent sheet; The cover plate is detachably installed on the base plate above the partition component, and the cover plate presses the bottom surface of the partition component against the top surface of the transparent sheet. The top surface of the cover plate is also provided with a flow channel opening facing the partition chamber.

2. The detachable microfluidic cell chip of claim 1, wherein: The top surface of the partition component abuts against the bottom surface of the cover plate, and the bottom surface of the partition component abuts against the top surface of the transparent sheet; And / or, the separator is made of PDMS, silicone or TPU.

3. The detachable microfluidic cell chip of claim 1, wherein: The cover plate is detachably connected to the base plate via a connector.

4. The detachable microfluidic cell chip of claim 3, wherein: The connector is a bolt. The top surface of the base plate outside the mounting cavity is provided with at least two screw holes. The cover plate is provided with at least two connecting holes that are respectively opposite to the screw holes. The bolt passes through the connecting holes and is screwed into the corresponding screw holes.

5. The detachable microfluidic cell chip of claim 1, wherein: The separating component is an elastic seal, and the thickness of the elastic seal is greater than the depth of the mounting cavity between the top surface of the transparent sheet and the top surface of the base plate.

6. The detachable microfluidic cell chip of claim 1, wherein: Each of the partitioned chambers is in communication with at least one of the flow channels.

7. The detachable microfluidic cell chip of claim 1, wherein: Each of the partition chambers is connected to at least one of the flow channels, and the two ends of the flow channels are connected to the top and bottom surfaces of the cover plate, respectively. Furthermore, each of the flow channels is provided with an isolation barrier.

8. The detachable microfluidic cell chip of claim 7, wherein: The sidewall of the partition component is provided with multiple through holes, and each partition chamber communicates with at least one of the through holes; The side wall of the base plate is provided with external through holes that correspond one-to-one with the through holes, and each external through hole is connected to one of the through holes.

9. The detachable microfluidic cell chip of claim 7, wherein: The isolation barrier is a hydrogel or a porous membrane.

10. The detachable microfluidic cell chip of claim 1, wherein: The bottom surface of the base plate is provided with a through groove communicating with the mounting chamber, and the size of the through groove is smaller than the size of the mounting chamber; And / or, the outer edge of the bottom surface of the transparent sheet abuts against the bottom surface of the mounting chamber next to the through groove.

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