Device for anesthetizing newborn mice with ice

Abstract

This utility model discloses a device for anesthetizing newborn mice by freezing. The device includes: a freezing body providing a low-temperature environment; a capture component for restraining the mouse, the capture component including: an enclosing portion surrounding 3 / 5 to 4 / 5 of the mouse's body in the circumferential direction; a grasping component disposed at one or both ends of the enclosing portion, the grasping component being a loop attached to the end of the enclosing portion for fitting the mouse's arm; and a supporting component for suspending the capture component on the freezing body, immersing the neck and below of the mouse restrained by the capture component in the low-temperature environment of the freezing body. This utility model has a simple structure, saves time and effort, can adapt to the body size of mice at different growth stages, effectively freezes newborn mice, rendering them immobile without causing death.

Description

Technical Field

[0001] This utility model relates to the field of biological and medical experimental devices, and more specifically to a device for anesthetizing newborn mice with ice. Background Technology

[0002] Mice are the most commonly used laboratory animals in biological and medical research, and intraocular injection in newborn mice is a common and important experimental procedure. The usual procedure involves manually stabilizing the mouse before inserting the needle. However, due to the small size of newborn mice, especially their tiny eyes, and their tendency to move violently and even bite during the procedure, intraocular injection in newborn mice is very difficult to perform, resulting in poor injection stability and low experimental efficiency. Currently, there is no dedicated rapid intraocular injection device for newborn mice on the market.

[0003] Therefore, there is currently a lack of a device in the field that can keep newborn mice still during eye injections. Utility Model Content

[0004] The purpose of this invention is to provide a device for freezing and anesthetizing newborn mice. This device has a simple structure, saves time and effort, can adapt to the size of mice at different growth stages, effectively freezes newborn mice, renders them immobile, and does not cause the mice to die.

[0005] This invention provides a device for anesthetizing newborn mice with ice, the device comprising: an ice-cooling body providing a low-temperature environment; a capture component for restraining the mouse, the capture component comprising: an enclosing portion surrounding 3 / 5 to 4 / 5 of the mouse's body in the circumferential direction; a grasping component disposed at one or both ends of the enclosing portion, the grasping component being a ring attached to the end of the enclosing portion for fitting the mouse's arm; and a supporting component for suspending the capture component on the ice-cooling body, immersing the neck and below of the mouse restrained by the capture component in the low-temperature environment of the ice-cooling body; the supporting component taking one of the following forms: a mechanical arm connected by a pivot; a relatively slidable sleeve secured by bolts; or the supporting component being a ramp structure, the ramp having a locking fastener on its inclined side for locking and securing a transverse support connected to the capture component.

[0006] In another preferred embodiment, the chiller body provides a low-temperature environment via an ice-water mixture.

[0007] In another preferred embodiment, the ice-cooling body utilizes semiconductor cooling to maintain the low-temperature environment of the ice-water mixture.

[0008] In another preferred embodiment, the chiller body provides a temperature environment of -2 to 5°C.

[0009] In another preferred embodiment, the capturing component is a rigid material capable of plastic deformation.

[0010] In another preferred embodiment, the capturing component includes, but is not limited to, iron wire, stainless steel wire, and copper wire.

[0011] In another preferred embodiment, the surrounding portion is an open ring.

[0012] In another preferred embodiment, the opening degree of the opening ring is adjustable.

[0013] In another preferred embodiment, the ring of the capturing component is, but is not limited to, a circular ring or an elliptical ring.

[0014] In another preferred embodiment, the area of ​​the ring of the capturing component is adjustable.

[0015] A device for anesthetizing newborn mice by freezing, the device comprising: a freezing body providing a low-temperature environment; a capture component for restraining the mouse, the capture component comprising: an enclosing portion surrounding 3 / 5 to 4 / 5 of the mouse's body in the circumferential direction; a grasping component disposed at one or both ends of the enclosing portion, the grasping component being a loop attached to the end of the enclosing portion for fitting the mouse's arm; and a supporting component for suspending the capture component on the freezing body, immersing the neck and below of the mouse restrained by the capture component in the low-temperature environment of the freezing body.

[0016] In another preferred embodiment, the chiller body provides a low-temperature environment via an ice-water mixture.

[0017] In another preferred embodiment, the chiller body provides a temperature environment of -2 to 5°C; preferably, a temperature environment of 0 to 4°C; more preferably, a temperature environment of 0 to 3°C.

[0018] In another preferred embodiment, the chiller body includes a temperature control component for maintaining a constant temperature in the low-temperature environment.

[0019] In another preferred embodiment, the temperature control component includes a cooling component, a temperature sensor, and a controller.

[0020] In another preferred embodiment, the cooling component is a semiconductor cooling component.

[0021] In another preferred embodiment, the temperature control component further includes a display screen for displaying the temperature environment provided by the chiller body in real time.

[0022] In another preferred embodiment, the display screen is also used to display the target temperature.

[0023] In another preferred embodiment, the capturing component is a rigid material capable of plastic deformation.

[0024] In another preferred embodiment, the capturing component is adjustable.

[0025] In another preferred embodiment, the capturing component includes, but is not limited to, iron wire, stainless steel wire, and copper wire.

[0026] In another preferred embodiment, the surrounding portion is an open ring; preferably, the opening degree of the open ring is adjustable.

[0027] In another preferred embodiment, the ring of the capturing component is, but is not limited to, a circular ring or an elliptical ring; preferably, the area of ​​the ring of the capturing component is adjustable.

[0028] In another preferred embodiment, the area of ​​the ring of the grasping component is 0.5-2 mm. 2 Preferably, 0.5-1.5mm 2 More preferably, 0.5-1mm 2 .

[0029] In another preferred embodiment, when a catcher is provided, one of the mouse's upper arms is placed in the catcher, and the other upper arm of the mouse is resting on the enclosure.

[0030] In another preferred embodiment, when two grasping devices are provided, both upper arms of the mouse are placed in the grasping devices.

[0031] In another preferred embodiment, the positional relationship between the grasping component and the ice-cooling body is adjustable via the supporting component.

[0032] In another preferred embodiment, the support component is in the form of a robotic arm connected by a pivot.

[0033] In another preferred embodiment, the support member is in the form of a relatively slidable sleeve that is fastened by bolts.

[0034] In another preferred embodiment, the support component is a ramp structure, and a locking fastener is provided on the inclined side of the ramp for locking and fixing the horizontal support bar connected to the capture component.

[0035] In another preferred embodiment, the engaging fasteners appear in pairs.

[0036] In another preferred embodiment, the support component includes multiple pairs of the engaging fasteners.

[0037] In another preferred embodiment, on one side, the lateral spacing between adjacent engaging fasteners is 3-50 mm; preferably, 5-15 mm; more preferably, 6-10 mm.

[0038] In another preferred embodiment, the slope of the ramp is 3-30 degrees; preferably, 5-20 degrees; more preferably, 7-10 degrees.

[0039] In another preferred embodiment, the same support component can support multiple capture components simultaneously.

[0040] In another preferred embodiment, the operator can directly hold the support component (e.g., a support component formed as an extendable rod) to control the positional relationship between the grabbing component and the ice-cold body.

[0041] In another preferred embodiment, the device includes a timer for recording the time a mouse is immersed in the ice-water mixture.

[0042] It should be understood that, within the scope of this utility model, the above-described technical features of this utility model and the technical features specifically described below (such as in the embodiments) can be combined with each other to form new or preferred technical solutions. Due to space limitations, they will not be described in detail here. Attached Figure Description

[0043] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0044] Figure 1 This is a schematic diagram of the structure of a device for anesthetizing newborn mice with ice in one embodiment of this utility model;

[0045] Figure 2 This is a schematic diagram of the structure of a device for anesthetizing newborn mice with ice in another embodiment of this utility model;

[0046] Figure 3 This is a schematic diagram of the structure of a device for anesthetizing newborn mice with ice in another example of this utility model.

[0047] The labels in each of the attached figures are as follows:

[0048] 1-Iced main body;

[0049] 2-Capture components;

[0050] 3-Enclosing section;

[0051] 4-Grab the components;

[0052] 5-Robotic arm;

[0053] 6-Pivot;

[0054] 7-Vertical bar;

[0055] 8-Casing;

[0056] 9-Horizontal bar;

[0057] 10-Slope;

[0058] 11-Snap-fit ​​fastener;

[0059] 12- Lateral support component. Detailed Implementation

[0060] Through extensive and in-depth research and screening, the inventors have developed for the first time a device for anesthetizing newborn mice with ice. The device provides a low-temperature environment that can freeze newborn mice into a stupor through the ice-cooling body, and the mouse is held by the grasping component so that its neck and below are immersed in the low-temperature environment. The part of the mouse immersed in the low-temperature environment is controlled and adjusted by the supporting component to accommodate mice of different sizes. Based on this, the present invention was completed.

[0061] The main advantages of this utility model include:

[0062] (a) It is easy to operate, improves experimental efficiency, simplifies the operator's work, and reduces the technical skill requirements for the operator;

[0063] (b) Physical hypothermia rendered newborn mice immobile, which, compared with chemical sedation, eliminated the influence of sedation on the experimental results.

[0064] (c) Only the neck and below of the mouse are immersed in the low temperature environment to freeze the mouse body to the maximum extent, without causing the mouse to choke on water or get ice water into its eyes, thus affecting the experimental structure.

[0065] (d) Because newborn mice grow rapidly, their body size can vary greatly within a few days. The device of this invention can be effectively applied to mice of different body sizes.

[0066] (e) By using semiconductor cooling (which utilizes the Peltier effect of semiconductor materials) and maintaining a low-temperature environment, on the one hand, this method can quickly obtain a low-temperature environment in a short time, and the effect is rapid; on the other hand, this method has low noise, which can alleviate the mice's tension during the experimental operation, making the experiment easier; in addition, this method has low energy consumption and is more economical for long-term use.

[0067] (f) makes the experimental results more accurate;

[0068] (g) The structure is simple and easy to manufacture.

[0069] The present invention will be further described below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, the accompanying drawings are schematic diagrams, and therefore the device and apparatus of the present invention are not limited to the size or scale of the schematic diagrams.

[0070] It should be noted that in the claims and specification of this patent, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one" does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0071] Example 1

[0072] The device for numbing newborn mice with ice in this embodiment is as follows: Figure 1 As shown. The device includes an ice-cooling main body 1, a capture component 2, and a support component.

[0073] The chiller body 1 is an open container that provides a low-temperature environment of 0–4°C through the ice-water mixture contained within it. The operator can set the temperature provided by the chiller body 1 as needed, for example, setting it to 0°C with an allowable error of ±1°C.

[0074] Preferably, the ice-cooling body 1 may include a temperature control component (not shown) for maintaining a constant temperature in the low-temperature environment. The temperature control component includes a refrigeration component, a temperature sensor, a controller, and a display screen, etc. The display screen is used to display the current temperature and target temperature in real time. The refrigeration component is a semiconductor refrigeration component, such as a TEC1-12706 semiconductor refrigeration chip, which is attached to the container wall of the ice-cooling body 1 for cooling.

[0075] The grasping component 2 is used to restrain the mouse. It is adjustable and is a rigid material that can undergo plastic deformation, including but not limited to iron wire, stainless steel wire, copper wire, etc. The grasping component 2 includes an enclosing part 3 and a grasping part 4. The enclosing part 3 is an open ring that surrounds 3 / 5 to 4 / 5 of the mouse's body in the circumferential direction, and the opening degree of the open ring is adjustable.

[0076] The gripping components 4 are located at both ends of the surrounding portion 3. The gripping components 4 are rings attached to the ends of the surrounding portion 3 and are used to snare the mouse's arms. The rings of the gripping components 2 are, but are not limited to, circular rings or elliptical rings, and the area of ​​the rings of the gripping components 2 is adjustable. Both of the mouse's upper arms are placed in the gripping components.

[0077] The support component suspends the capture component 2 on the cooling body 1, immersing the neck and below of the mouse restrained by the capture component 2 in the low-temperature environment of the cooling body 1. The positional relationship between the capture component 2 and the cooling body 1 is adjustable via the support component.

[0078] In this embodiment, the support component is in the form of a robotic arm 5 connected via a pivot 6. For example... Figure 1 As shown, the support component includes two robotic arms 5, which are connected and fixed together by a pivot 6. The end of the upper arm is fixedly connected to the grasping component 2 via a vertical rod 7. The height is adjusted by rotating the pivot 6, similar to the adjustment arm of a table lamp. In one embodiment, the pivot 6 may be, but is not limited to, the pivot structure that can be stopped and positioned as described in the patent filed on December 8, 1992, with patent number 92243011.X and utility model title "Stop-Positioning Pivot Structure". By replacing one of the two robotic arms 5 with the shell cover in the aforementioned utility model patent, and replacing the other of the two robotic arms 5 with the shell in the aforementioned utility model patent, that is, by fixing one robotic arm to the fixing frame in the aforementioned utility model patent and fixing the other robotic arm to the rotating shaft in the aforementioned utility model patent, the pivoting and position fixing between the two robotic arms can be achieved, thereby allowing the robotic arms to be stably positioned in a predetermined position, thus achieving stable injection into the mouse eye.

[0079] The support component can be independently mounted or fixed to the frozen body. Preferably, the support component is slidable relative to the container, for example, sliding along the side wall of the container. The number of robotic arms 5 can also be three or more, with adjacent robotic arms 5 connected by pivots 6. Multiple robotic arms 5 provide greater degrees of freedom of movement (e.g., movement in the X, Y, and Z directions).

[0080] Example 2

[0081] The device for anesthetizing newborn mice with ice in this embodiment is similar to that in Embodiment 1, except that the support component in this embodiment is in the form of a sleeve, such as... Figure 2As shown. A pair of sleeves 8 are set on opposite sides of the ice-cooling body 1. A horizontal bar 9 connects the pair of sleeves 8 from above. The upper end of the vertical bar 7 is fixed to the horizontal bar 9 and can slide relative to the horizontal bar 9, while the lower end is fixedly connected to the grasping component 2. Each side has two sleeves 8, with the inner tube on top and the outer tube on the bottom, connected to the ice-cooling body 1. The inner and outer tubes can slide relative to each other. The two sleeves 8 are fixed in relative position by external bolts (not shown). The external bolts can be set only between the two sleeves 8 on one side, or external bolts can be set on both sides for fixation. It is also possible to set sleeves 8 on only one side. It is also possible to control the height or degree of freedom of movement by using three or more sleeves 8.

[0082] Furthermore, unlike Embodiment 1, in this embodiment, the grasping component 4 is only located at one end of the surrounding portion 3. In use, one of the mouse's upper arms is inserted into the grasping component, while the other upper arm of the mouse rests on the surrounding portion 3.

[0083] Example 3

[0084] The device for anesthetizing newborn mice with ice in this embodiment is similar to that in Embodiment 2, except that the supporting component in this embodiment is a ramp structure, such as... Figure 3 As shown. The ramp 10 is set on the ice-cold body 1, or is integrated with the ice-cold body 1. The slope of the ramp 10 is approximately 10 degrees, which is the angle formed by the inclined side of the ramp 10 and the liquid surface of the ice-water mixture (or the bottom surface of the container of the ice-cold body 1 when placed horizontally). Multiple pairs of locking fasteners 11 are provided on the inclined side of the ramp 10 for locking and fixing the lateral support 12 connected to the grasping component 2. The lateral support 12 is fixedly connected to the grasping component 2. On one side, the lateral spacing between adjacent locking fasteners 11 is 10 mm. With the setting of multiple pairs of locking fasteners 11, the height of the grasping component 2 from the liquid surface in the container can be selected at different levels, and the operator can select the appropriate locking fastener setting according to the size of the mouse.

[0085] All documents mentioned in this invention are incorporated herein by reference as if each document were individually incorporated by reference. Furthermore, it should be understood that after reading the foregoing teachings of this invention, those skilled in the art can make various alterations or modifications to this invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. An apparatus for ice-bathing anaesthetized neonatal mice, characterized in that, The device includes: The main body of the ice-cooling system provides a low-temperature environment; A capture component for restraining the mouse, the capture component comprising: The surrounding portion, which circumferentially surrounds 3 / 5 to 4 / 5 of the mouse's body; and A gripping component, wherein the gripping component is disposed at one or both ends of the surrounding portion, the gripping component being a ring attached to the end of the surrounding portion for fitting the arm of the mouse; and A support component is provided for suspending the capture component on the ice-cooling body, immersing the neck and below of the mouse restrained by the capture component in the low-temperature environment of the ice-cooling body; The support component takes one of the following forms: In the form of a robotic arm connected by a pivot; A sleeve that is relatively sliding and fastened by bolts; or The support component is a ramp structure, and a locking and fixing component is provided on the inclined side of the ramp for locking and fixing the transverse support component connected to the capture component.

2. The apparatus of claim 1, wherein, The ice-cooling unit provides a low-temperature environment through a mixture of ice and water.

3. The apparatus as described in claim 2, characterized in that, The ice-cooling unit uses semiconductor cooling to maintain the low-temperature environment of the ice-water mixture.

4. The apparatus as claimed in claim 1, characterized in that, The main body of the ice-cooling system provides a temperature environment of -2 to 5℃.

5. The apparatus as claimed in claim 1, characterized in that, The capturing component is a rigid material capable of plastic deformation.

6. The apparatus as claimed in claim 5, characterized in that, The capture components include, but are not limited to, iron wire, stainless steel wire, and copper wire.

7. The apparatus as claimed in claim 5, characterized in that, The surrounding portion is an open ring.

8. The apparatus of claim 7, wherein, The opening degree of the open ring is adjustable.

9. The apparatus of claim 5, wherein, The ring of the capturing component is, but is not limited to, a circular ring or an elliptical ring.

10. The apparatus of claim 9, wherein, The area of ​​the ring in the capture component is adjustable.

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