An activity assay kit for sub-pluripotent stem cells

Abstract

This utility model relates to the field of reagent kit technology, specifically disclosing a reagent kit for detecting the activity of sub-pluripotent stem cells. The kit includes: a reagent kit with a protective cover rotatably connected to its outer surface, a protective pad fixedly connected to the inner surface of the protective cover, and a tube-removing assembly movably connected inside the kit. The assembly includes a base frame, a limiting seat fixedly connected to the upper surface of the base frame, and a sliding groove through the side surface of the limiting seat. A spade is fixedly connected to the inner wall of the sliding groove. Through the linkage of a push rod, a push block, and a fixed seat, when the push block is pulled inward, the push rod pushes the base frame to compress a spring column, causing the spade in the sliding groove of the limiting seat to lift the reagent tube and push it vertically out along the fixed frame, achieving quick tube removal with one hand. After releasing the push block, the spring column elastically resets, causing the base frame to return to its original position, automatically completing the reagent tube positioning, effectively improving the problems of cumbersome tube removal and easy contamination in traditional reagent kits.

Description

Technical Field

[0001] This invention relates to the field of reagent kit technology, specifically to an activity detection kit suitable for sub-pluripotent stem cells. Background Technology

[0002] Subpluripotent stem cell activity testing refers to the core process of evaluating the biological activity and functional status of this type of stem cell (which has the ability to differentiate into three germ layer tissues but cannot develop into a complete individual) through a series of experiments such as proliferation capacity, differentiation potential, and surface marker analysis.

[0003] Existing activity assay kits for sub-pluripotent stem cells mostly use open slots or fixed supports to store reagent tubes, requiring manual insertion and removal during use. This is not only cumbersome and inefficient, but also prone to causing the reagent tubes to tilt or slip due to improper force, increasing the risk of sample contamination. Therefore, we propose an activity assay kit suitable for sub-pluripotent stem cells. Utility Model Content

[0004] The purpose of this invention is to provide an activity detection kit for subpluripotent stem cells, in order to solve the problem that the activity detection kits for subpluripotent stem cells mentioned in the background art mostly use open slots or fixed supports to store reagent tubes, which require manual insertion and removal during the process. This is not only cumbersome and inefficient, but also prone to causing the reagent tubes to tilt or slip due to improper force, increasing the risk of sample contamination.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a kit for detecting the activity of sub-pluripotent stem cells, comprising a kit, a protective cover rotatably connected to the outer surface of the kit via a hinge, the protective cover being rotatable from 0 to 180 degrees around the hinge to complete the opening and closing operation of the kit; a protective pad is fixedly connected to the inner surface of the protective cover by adhesive, the protective pad being made of elastic rubber material, and when the protective cover is closed, the protective pad can fit against the internal components of the kit, playing a buffering and protective role; a tube-taking assembly is movably connected inside the kit body; The tube dispensing assembly includes a base frame made of rigid plastic, in the shape of a rectangular plate, the length of which matches the internal width of the reagent kit. A limiting seat, a hollow cuboid structure, is fixedly connected to the upper surface of the base frame via screws to limit the position of the reagent tubes. A groove runs through the side surface of the limiting seat along its length, the length of which matches the height of the limiting seat. A spatula is fixedly connected to the inner wall of the groove, the spatula being inclined at an angle of 30-45 degrees, its top end flush with the top of the groove, and its bottom end extending to the middle of the limiting seat. A connecting block, a cube structure, is fixedly connected to the side surface of the base frame. A spring post is fixedly connected to the end of the connecting block away from the base frame, and a fixing plate, also rectangular in shape, is fixedly connected to the end of the spring post away from the connecting block.

[0006] The end of the fixing plate away from the spring column is fixedly connected to the inner wall of the reagent kit body by welding; the connecting block, spring column and fixing plate are horizontally fixedly connected between the base frame and the inner wall of the reagent kit body in two sets, and are symmetrically distributed about the central axis of the base frame to ensure that the base frame is subjected to uniform force during movement.

[0007] The reagent tube is movably connected inside the limiting seat, and a fixing frame is slidably connected to the top side surface of the reagent tube. The side surface of the fixing frame is fixedly connected to the top inner wall of the reagent kit.

[0008] The bottom of the base frame is fixedly connected to a connecting plate, and the upper surface of the connecting plate is movably connected to a guide plate. The end of the guide plate away from the connecting plate is fixedly connected to the bottom of the reagent kit. The connecting plate and the guide plate are arranged symmetrically in two sets between the bottom of the base frame and the bottom of the reagent kit.

[0009] The bottom of the base frame is fixedly connected to a base, and a push rod is fixedly connected to the outer surface of the base. The push rod is connected through the inner and outer ends of the bottom side of the reagent kit, and a push block is fixedly connected to the end of the push rod away from the base.

[0010] The outer surface of the push rod is movably connected to a positioning ring, and the outer surface of the positioning ring is fixedly connected to the bottom outer surface of the reagent kit.

[0011] This utility model has at least the following beneficial effects: 1. In use, this utility model utilizes the linkage between the push rod, push block, and fixed base. When the push block is pulled inward, the push rod pushes the base frame to compress the spring column, causing the spade plate in the sliding groove of the limiting base to lift the reagent tube and push it vertically out along the fixed frame, enabling quick tube removal with one hand. After releasing the push block, the spring column elastically resets, causing the base frame to return to its original position, automatically completing the positioning of the reagent tube. Furthermore, the reset speed gradually decreases as the deformation recovers, achieving a "soft landing" return, preventing rigid collisions between the base frame and the inner wall of the reagent kit. This protects the components and reduces noise. During tube removal, the hand only contacts the top screw cap of the reagent tube, avoiding contact with the lower half of the reagent tube, effectively improving the problems of cumbersome and easily contaminated tube removal in traditional reagent kits.

[0012] 2. In use, the protective cover is rotatably connected to the reagent kit. When closed, the protective pad fits tightly inside to form a sealed space, isolating dust and moisture. The base frame forms a shock-absorbing structure through the guide plate, spring column and fixing plate, and the positioning ring limits the push rod. During transportation or storage, it buffers vibration, avoids shaking and collision of reagent tubes, and ensures that the activity of the sub-pluripotent stem cell reagent is not affected by the external environment. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a three-dimensional schematic diagram of the tube-taking assembly of this utility model; Figure 3 The structure of this utility model Figure 2 Enlarged view of point A in the middle; Figure 4 This is a top view of a partial connection structure between the structural base and the fixed seat of this utility model.

[0014] In the diagram: 1. Reagent kit; 2. Protective cap; 3. Protective pad; 4. Tube dispensing assembly; 41. Base frame; 42. Limiting seat; 43. Slide opening; 44. Spatula; 45. Reagent tube; 46. Fixing frame; 47. Connecting plate; 48. Guide plate; 49. Connecting block; 410. Spring post; 411. Fixing plate; 412. Fixing seat; 413. Push rod; 414. Positioning ring; 415. Push block. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model. Example 1

[0016] Please see Figure 1-4 This utility model provides a technical solution: an activity detection kit for sub-pluripotent stem cells, comprising a kit 1, a protective cover 2 rotatably connected to the outer surface of the kit 1 via a hinge, the protective cover 2 being rotatable from 0 to 180 degrees around the hinge to complete the opening and closing operation of the kit 1; a protective pad 3 is fixedly connected to the inner surface of the protective cover 2 by adhesive, the protective pad 3 being made of elastic rubber, and when the protective cover 2 is closed, the protective pad 3 can fit against the internal components of the kit 1, playing a buffering and protective role; a tube extraction assembly 4 is movably connected inside the kit 1; The tube dispensing assembly 4 includes a base frame 41, which is made of rigid plastic and has a rectangular plate-like structure. The length of the base frame 41 is adapted to the internal width of the reagent kit 1. The upper surface of the base frame 41 is fixedly connected to a limiting seat 42 by screws. The limiting seat 42 is a hollow cuboid structure used to limit the reagent tube 45. The limiting seat 42 has three silicone anti-slip strips distributed along the axial direction. The anti-slip strips have a semi-circular cross section. When the reagent tube 45 is inserted, the anti-slip strips fit tightly against the tube wall to prevent the reagent tube from shifting due to shaking when not in operation. At the same time, the silicone material has good chemical inertness and will not react with common reagents. A groove 43 is formed along the length of the side surface of the limiting seat 42, and the length is the same as the height of the limiting seat 42. A spade 44 is fixedly connected to the inner wall of the groove 43. The spade 44 is inclined at an angle of 30-45 degrees. The top of the spade 44 is flush with the top of the groove 43, and the bottom extends to the middle of the limiting seat 42. The connection between the inner wall of the groove 43 and the spade 44 is rounded to avoid the right angle structure from scratching the outer wall of the reagent tube 45. The top of the spade 44 is rounded to ensure that it can generate sufficient lifting force when in contact with the bottom of the reagent tube 45, without damaging the tube wall due to the sharp corners. A connecting block 49 is fixedly connected to the side surface of the base frame 41. The connecting block 49 has a cubic structure. A spring column 410 is fixedly connected to the end of the connecting block 49 away from the base frame 41. A fixing plate 411 is fixedly connected to the end of the spring column 410 away from the connecting block 49. The fixing plate 411 has a rectangular plate structure.

[0017] The protective cap 2 has an integrally formed flange structure at its edge, with a trapezoidal nitrile rubber sealing ring embedded inside the flange. When the protective cap 2 is closed, the flange precisely engages with the groove at the opening of the reagent kit 1, compressing the sealing ring and forming the first sealing barrier. The protective pad 3 is made of foamed rubber, which forms a buffer structure when it fits against the top of the reagent tube 45. It can absorb external vibrations and prevent excessive compression that could cause deformation of the reagent tube. Together with the sealing ring, it achieves a dual protective effect of "soft buffering + hard sealing". In use, through the linkage of push rod 413, push block 415 and fixed base 412, when push block 415 is pulled inward, push rod 413 pushes base frame 41 to compress spring column 410, so that spatula 44 in sliding groove 43 of limit seat 42 lifts reagent tube 45 and pushes it out vertically along fixed frame 46, realizing quick tube removal by one hand; after releasing push block 415, spring column 410 elastically resets and drives base frame 41 to return to position, automatically completing the positioning of reagent tube 45, effectively improving the problem of cumbersome tube removal and easy contamination of traditional reagent kit 1.

[0018] The end of the fixing plate 411 away from the spring column 410 is fixedly connected to the inner wall of the reagent kit 1. The connecting block 49, the spring column 410 and the fixing plate 411 are horizontally fixedly connected in two sets between the base frame 41 and the inner wall of the reagent kit 1.

[0019] A reagent tube 45 is movably connected inside the limiting seat 42. A fixing bracket 46 is slidably connected to the top side surface of the reagent tube 45. The side surface of the fixing bracket 46 is fixedly connected to the top inner wall of the reagent kit 1. The inner side of the fixing bracket 46 has an arc-shaped groove that matches the reagent tube 45. A silicone pad is attached to the inner wall. The surface of the silicone pad is frosted, which can enhance the friction with the reagent tube and avoid tube wall wear caused by hard contact. The connection between the fixing bracket 46 and the top inner wall of the reagent kit 1 adopts a detachable snap-fit ​​structure, which makes it easy to replace the fixing bracket of different specifications according to the diameter of the reagent tube 45, thereby improving the versatility of the reagent kit.

[0020] A connecting plate 47 is fixedly connected to the bottom of the base frame 41, and a guide plate 48 is movably connected to the upper surface of the connecting plate 47. The connecting plate 47 and the guide plate 48 at the bottom of the base frame 41 form a sliding guide mechanism, which ensures smooth sliding without jamming and limits the lateral displacement of the base frame 41. The end of the guide plate 48 away from the connecting plate 47 is fixedly connected to the bottom of the reagent kit 1. The connecting plate 47 and the guide plate 48 are arranged in two symmetrical sets between the bottom of the base frame 41 and the bottom of the reagent kit 1. The two sets of guide mechanisms are symmetrically distributed along the central axis of the base frame 41, so that the base frame 41 is subjected to balanced force during movement, further improving the stability of movement.

[0021] A base 412 is fixedly connected to the bottom of the base frame 41. A push rod 413 is fixedly connected to the outer surface of the base 412. The push rod 413 is connected through the inner and outer ends of the bottom side of the reagent kit 1. A push block 415 is fixedly connected to the end of the push rod 413 away from the base 412.

[0022] The outer surface of the push rod 413 is movably connected to a positioning ring 414, and the outer surface of the positioning ring 414 is fixedly connected to the bottom outer surface of the reagent kit 1.

[0023] In use, the reagent tube 45 is placed vertically inside the limiting seat 42, with its top fixed to the inner wall of the top of the reagent kit 1 via the fixing bracket 46, and its bottom contacting the base frame 41. The side of the base frame 41 away from the fixing seat 412 is connected to the inner wall of the reagent kit 1 via the connecting block 49, the spring column 410, and the fixing plate 411, with the spring column 410 in a relaxed state; the bottom connecting plate 47 slides along the guide plate 48 to ensure stable movement of the base frame 41; the push rod 413 passes through the bottom of the reagent kit 1, with the push block 415 at one end located on the outside, and the positioning ring 414 is sleeved on the push rod 413 to limit its movement trajectory.

[0024] When reagent tube 45 needs to be retrieved, the push block 415 is pulled inward, causing the push rod 413 to move horizontally. The push rod 413 pushes the fixed seat 412 and the base frame 41 to squeeze the spring column 410 inward. When the base frame 41 moves with the fixed seat 412, the shovel plate 44 in the sliding groove 43 on the side surface of the limiting seat 42 moves synchronously, gradually lifting the bottom of the reagent tube 45, so that the reagent tube 45 is pushed vertically upward along the fixed frame 46. The operator can directly grasp the reagent tube 45 to take it out. During the tube retrieval operation, when the push block 415 is pulled inward, the pulling force transmitted by the push rod 413 causes the base frame 41 to move against the elastic force of the spring column 410. At this time, the contact point between the shovel plate 44 and the bottom of the reagent tube 45 forms a fulcrum. The horizontal displacement is converted into vertical lift through the tilt angle of 30-45 degrees. The relationship between the lift force F and the horizontal pulling force F0 is F=F0×tanA (A is the tilt angle of the shovel plate 44).

[0025] After use, place the reagent tube 45 back into the limiting seat 42, release the push block 415, and the spring column 410 releases its elastic potential energy, pushing the base frame 41 back to its original position along the guide plate 48. The shovel plate 44 then resets, allowing the reagent tube 45 to return to its vertical position, and the top fixing bracket 46 securely clamps it again. When the protective cover 2 is closed, the protective pad 3 adheres to the inside of the reagent kit 1, isolating it from external vibrations and dust. At the same time, the rotating connection structure between the protective cover 2 and the reagent kit 1 ensures a tight seal, preventing the reagent from being affected by the external environment and reducing its activity.

[0026] The working process for this application is as follows: In the initial state, the spring column 410 is in a naturally relaxed state, the base 41 is located at the inner limit position of the reagent kit 1, the bottom of the reagent tube 45 is in contact with the upper surface of the base 41, the silicone pad of the fixing frame 46 is tightly attached to the outer wall of the reagent tube 45, and the whole is in a stable storage state. At this time, the protective cover 2 is closed.

[0027] During the tube removal operation, the operator holds the side of reagent kit 1 with one hand. The push block 415 drives the push rod 413 to move axially along the positioning ring 414. The push rod 413 pushes the fixed seat 412 and the base frame 41 to move inward along the guide plate 48. The T-shaped groove of the connecting plate 47 slides along the T-shaped protrusion of the guide plate 48 to ensure that the base frame 41 moves horizontally without tilting. During the movement of the base frame 41, the spring column 410 is squeezed. The spring compression increases linearly with the movement distance. At this time, the limiting seat 42 moves with the base frame 41. The spatula 44 gradually contacts the bottom of the reagent tube 45. The horizontal displacement is converted into vertical lifting force, so that the reagent tube 45 rises vertically along the slot of the fixed frame 46. When the top of the reagent tube 45 exceeds the upper edge of the reagent kit 1, the operator can remove the reagent tube 45 by pinching the upper part of the reagent tube 45. During the tube removal process, the hand only touches the top screw cap of the reagent tube 45 to avoid touching the lower half of the reagent tube.

[0028] After use, align the reagent tube 45 with the center hole of the limiting seat 42 and insert it vertically until it contacts the bottom shovel plate 44. At this time, the upper part of the reagent tube 45 naturally embeds into the slot of the fixing frame 46. Slowly release the push block 415, and the spring column 410 releases elastic potential energy, pushing the base frame 41 to move in the opposite direction along the guide plate 48. During the reset process, the spring force gradually decreases, so the reset speed gradually decreases as the deformation recovers, achieving a "soft landing" reset, preventing the base frame 41 from rigidly colliding with the inner wall of the reagent kit 1, protecting the components and reducing noise. When the base frame 41 resets to the initial position, the shovel plate 44 disengages from the bottom of the reagent tube 45 and is placed stably on the upper surface of the base frame 41, completing the automatic positioning.

[0029] When the protective cap 2 is closed, the protruding edge of the protective cap 2 is embedded into the sealing groove of the reagent kit 1. At this time, the protective pad 3 contacts the top of the reagent tube 45. The automatic reset of the spring column 410 avoids contact when manually adjusting the position of the reagent tube. Combined with the sealing design of the protective cap 2, the safety of reagent storage is improved.

[0030] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0031] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An activity detection kit suitable for use with a sub- totipotent stem cell, comprising: The reagent kit is characterized in that: a protective cover is rotatably connected to the outer surface of the reagent kit, a protective pad is fixedly connected to the inner surface of the protective cover, and a tube-taking assembly is movably connected to the inside of the reagent kit; The tube-retrieving assembly includes a base frame, a limiting seat is fixedly connected to the upper surface of the base frame, a sliding groove is opened through the side surface of the limiting seat, a shovel plate is fixedly connected to the inner wall of the sliding groove, a connecting block is fixedly connected to the side surface of the base frame, a spring column is fixedly connected to the end of the connecting block away from the base frame, and a fixing plate is fixedly connected to the end of the spring column away from the connecting block.

2. The active detection kit for totipotential stem cells according to claim 1, wherein: The end of the fixing plate away from the spring column is fixedly connected to the inner wall of the reagent kit. The connecting block, spring column and fixing plate are horizontally fixedly connected in two sets between the base frame and the inner wall of the reagent kit.

3. The active detection kit for totipotential stem cells according to claim 1, wherein: The reagent tube is movably connected inside the limiting seat, and a fixing frame is slidably connected to the top side surface of the reagent tube. The side surface of the fixing frame is fixedly connected to the top inner wall of the reagent kit.

4. The active detection kit for totipotential stem cells according to claim 1, wherein: A connecting plate is fixedly connected to the bottom of the base frame, and a guide plate is movably connected to the upper surface of the connecting plate. The end of the guide plate away from the connecting plate is fixedly connected to the bottom of the reagent kit. The connecting plate and the guide plate are arranged symmetrically in two sets between the base frame and the bottom of the reagent kit.

5. The active detection kit for totipotential stem cells according to claim 1, wherein: A fixed base is fixedly connected to the bottom of the base frame, and a push rod is fixedly connected to the outer surface of the fixed base. The push rod is connected through the inner and outer ends of the bottom side of the reagent kit, and a push block is fixedly connected to the end of the push rod away from the fixed base.

6. The activity detection kit for sub-pluripotent stem cells according to claim 5, characterized in that: A positioning ring is movably connected to the outer surface of the push rod, and the outer surface of the positioning ring is fixedly connected to the bottom outer surface of the reagent kit.

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