A Multiplex PCR Detection Kit for Mastitis-Causing Bacteria in Dairy Goats
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-15
- Publication Date
- 2026-08-14
AI Technical Summary
这种密集的联排放置结构会导致实际操作中的不便——当需要拿取时，多个联排试剂管紧密聚集，难以单独取出某一组，进而对检测操作的流畅性造成阻碍，因此，针对上述问题提出一种奶山羊乳房炎致病菌多重PCR检测试剂盒
1、通过设置的隔板，可以将存放盒的中部分割成多个存放区域，可以对试剂管进行分类存放，再配合上设置的固定块、圆杆、导向板、连接板与连接壳，可以在打开每个存放区域的单独盖板的同时，可以将内部的试剂管送出来，是拿取试剂管的空间扩大，提高了便捷性。
Smart Images
Figure CN224632222U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of pathogen detection kits, and in particular to a multiplex PCR detection kit for pathogens causing mastitis in dairy goats. Background Technology
[0002] Polymerase chain reaction (PCR) is a molecular biology technique based on the principle of DNA replication: using a DNA template, with the participation of DNA polymerase, nucleotide substrates, and other substances, a target DNA fragment is amplified to a quantity sufficient for structural and functional analysis through a specific reaction. This technique has significant value in the clinical field, especially in the rapid diagnosis of bacterial infectious diseases. Its core principle is the specific amplification of DNA fragments between two known sequences, a process similar to the replication mechanism of natural DNA.
[0003] In livestock testing, PCR technology has been applied to the detection of mastitis pathogens in dairy goats. Multiplex PCR detection, with its advantage of "simultaneous detection of multiple targets," can achieve accurate detection while ensuring high efficiency and economy. The test kit is the core tool in this process.
[0004] However, existing multiplex PCR detection kits have certain limitations: their function is limited to the placement and packaging of reagent tubes. Since multiplex PCR reagent tubes are mostly cylindrical or conical and need to hold different reagents separately, they are usually arranged side-by-side when placed in the kit. This dense arrangement leads to inconvenience in actual operation—when retrieving a particular set, the multiple tubes are tightly packed together, making it difficult to remove a single set, thus hindering the smoothness of the detection process. Therefore, to address these issues, a multiplex PCR detection kit for the pathogen causing mastitis in dairy goats is proposed. Utility Model Content
[0005] This utility model addresses the shortcomings of existing technologies by providing the following technical solution: A multiplex PCR detection kit for mastitis pathogens in dairy goats includes a storage box. Multiple partitions are fixedly connected to the center of the storage box. A fixing frame is slidably connected to the center of the storage box. A fixing block is fixedly connected to the top of the storage box. A cover plate is rotatably connected to the outer wall of the top of the fixing block. A through groove is formed in the side wall of the storage box. A connecting shell is slidably connected to the center of the through groove. One end of the connecting shell is fixedly connected to the side wall at the bottom of the fixing frame. A connecting plate is fixedly connected to the other end of the connecting shell. A guide plate is fixedly connected to the top side of the end of the connecting plate. A V-shaped groove is formed in the side wall at the top of the guide plate. A round rod is rotatably connected to the side wall of the cover plate. The outer wall of the round rod is slidably connected to the center of the V-shaped groove.
[0006] The partitions allow the storage box to be divided into multiple storage areas, enabling the reagent tubes to be stored in categories. Combined with the fixed blocks, round rods, guide plates, connecting plates, and connecting shells, the reagent tubes inside can be sent out while opening the individual cover of each storage area, thus expanding the space for retrieving reagent tubes and improving convenience.
[0007] As an improvement to the above technical solution, the side wall of the storage box is slidably connected to the bottom side of the connecting plate with a push bar, the middle of the push bar is slidably connected to a squeezing block, the bottom of the squeezing block is fixedly connected to a rectangular plate, the side wall of the push bar is fixedly connected to a slider, the side wall of the storage box is provided with a second sliding groove, and the outer wall of the slider is slidably connected to the middle of the second sliding groove.
[0008] As an improvement to the above technical solution, a sliding plate is slidably connected to the middle of the connecting shell, a limiting block is fixedly connected to the side wall of the end of the sliding plate, the outer wall of the limiting block is slidably connected to the middle of the connecting shell, a limiting groove is opened in the middle of the through groove, the limiting block is inserted into the middle of the limiting groove, and an L-shaped push plate is fixedly connected to the side wall of the other end of the sliding plate, the outer wall of the L-shaped push plate is slidably connected to the middle of the connecting plate.
[0009] As an improvement to the above technical solution, a spring is fixedly connected to the side wall of the end of the slide plate, and the other end of the spring is fixedly connected to the middle of the connecting shell. Multiple sets of springs are provided, with one end of each set fixedly connected to the side wall of the other end of the slide plate, and the other end of each set fixedly connected to the middle of the connecting plate. As an improvement to the above technical solution, a protective plate is fixedly connected to the top of the connecting shell, and a sliding groove is provided on the top side of the middle of the through groove, and the outer wall of the protective plate is slidably connected to the middle of the sliding groove.
[0010] As an improvement to the above technical solution, multiple sets of the cover plate, fixing block, round rod, guide plate, connecting plate, and protective plate are provided, and the multiple sets of cover plates, fixing blocks, round rods, guide plates, connecting plates, and protective plates are linearly and uniformly arranged on the side wall of the storage box, and the multiple sets of fixing blocks are fixed to the top of the partition.
[0011] As an improvement to the above technical solution, the top of the extrusion block is set as a slope.
[0012] The beneficial effects of this utility model are: 1. The partitions can divide the middle of the storage box into multiple storage areas, allowing for the categorized storage of reagent tubes. Combined with the fixed blocks, round rods, guide plates, connecting plates, and connecting shells, the reagent tubes inside can be sent out while opening the individual cover of each storage area, expanding the space for retrieving reagent tubes and improving convenience.
[0013] By using the coordination between the push bar, the squeezing block, the rectangular plate and the slider, all the reagent tubes can be pushed out at the same time. This eliminates the need for staff to push each reagent tube out one by one when they need to remove all the reagent tubes from the storage box, thus improving convenience. Attached Figure Description
[0014] Figure 1 This is a perspective view of the overall device of this utility model; Figure 2 This is a three-dimensional sectional view of the storage box and cover plate in this utility model. Figure 3 This is a three-dimensional view of the storage box and the push bar in this utility model. Figure 4 This is a three-dimensional sectional view of the guide plate and cover plate in this utility model. Figure 5 In this utility model Figure 4 A magnified 3D view of region A.
[0015] Reference numerals: 1. Storage box; 2. Cover plate; 21. Fixing block; 22. Round rod; 23. Guide plate; 24. V-groove; 25. Connecting plate; 26. Protective plate; 27. Connecting shell; 28. L-shaped push plate; 29. Limiting block; 210. Slide plate; 211. Spring; 3. Push bar; 31. Extrusion block; 32. Rectangular plate; 33. Slider; 4. Through groove; 5. Partition plate; 6. Fixing frame; 7. Slide groove one; 8. Limiting groove; 9. Slide groove two. Detailed Implementation
[0016] To make the objectives, technical solutions, and advantages of this utility model clearer, the following provides a more detailed description of the utility model. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of the utility model.
[0017] Please refer to Figure 1 - Figure 5As shown, this utility model provides a multiplex PCR detection kit for mastitis pathogens in dairy goats, including a storage box 1. Multiple sets of partitions 5 are fixedly connected to the middle of the storage box 1. A fixing frame 6 is slidably connected to the middle of the storage box 1. A fixing block 21 is fixedly connected to the top of the storage box 1. A cover plate 2 is rotatably connected to the outer wall of the top of the fixing block 21. A through groove 4 is formed on the side wall of the storage box 1. A connecting shell 27 is slidably connected to the middle of the through groove 4. The end of the connecting shell 27 is fixedly connected to the fixing frame 6. On the bottom sidewall, a connecting plate 25 is fixedly connected to the other end of the connecting shell 27. A guide plate 23 is fixedly connected to the top side of the end of the connecting plate 25. A V-shaped groove 24 is formed on the top sidewall of the guide plate 23. A round rod 22 is rotatably connected to the sidewall of the cover plate 2. The outer wall of the round rod 22 is slidably connected to the middle of the V-shaped groove 24. During operation, the middle of the storage box 1 is divided into multiple partitions by multiple sets of partitions 5, and a set of fixing brackets 6 is set in the middle of each partition. In this way, the storage box 1 can be divided into multiple partitions. Reagent tubes of the same type are stored separately. When a reagent tube needs to be removed from the middle of storage box 1, the connecting plate 25 can be pushed. The connecting plate 25 will drive the fixing frame 6 to move through the connecting shell 27. The fixing frame 6 will drive the reagent tube installed in the middle to move. At the same time, the connecting plate 25 will drive the guide plate 23 to move. The outer wall of the round rod 22 will slide in the middle of the V-shaped groove 24 and move along the slope of the V-shaped groove 24. Since the cover plate 2 and the fixing block 21 are rotatably connected, when the round rod 22 moves, it will drive the cover plate 2 to rotate on the outer wall at the top of the fixing block 21. At the same time, the round rod 22 will rotate on the side wall of the cover plate 2 for movement adjustment to ensure smooth cooperation between the structures. In this way, when the reagent tube is sent out from the middle of storage box 1, the cover plate 2 will flip open at the same time, improving convenience. The retrieval space for the reagent tube moved out from the middle of storage box 1 will be increased, and the staff can easily take out the reagent tube, improving convenience.
[0018] Furthermore, such as Figure 1 - Figure 3As shown, a pusher bar 3 is slidably connected to the side wall of the storage box 1 at the bottom of the connecting plate 25. A slider 33 is fixedly connected to the side wall of the pusher bar 3. A second groove 9 is opened on the side wall of the storage box 1. The outer wall of the slider 33 is slidably connected to the middle of the second groove 9. A squeezing block 31 is slidably connected to the middle of the pusher bar 3. A rectangular plate 32 is fixedly connected to the bottom of the squeezing block 31. During operation, when all the reagent tubes in the middle of the storage box 1 need to be taken out together, the rectangular plate 32 can be pushed to move. The rectangular plate 32 will simultaneously drive the pusher bar 3 and the squeezing block 31 to move. When the slider 33 slides in the middle of the slide groove 9, it ensures the stability of the push bar 3 until the push bar 3 contacts multiple sets of connecting plates 25 at the same time. Then, the rectangular plate 32 is pushed, and the rectangular plate 32 pushes the connecting plate 25 to move through the push bar 3. The connecting plate 25 drives the fixing frame 6 to move through the connecting shell 27. At the same time, the cover plate 2 is flipped open through the guide plate 23. In this way, all the reagent tubes in the middle of the storage box 1 can be moved out from the middle of the storage box 1 at the same time. There is no need for staff to push out different types of reagent tubes one by one, which improves convenience.
[0019] Furthermore, such as Figure 2 and Figure 4 - Figure 5 As shown, a sliding plate 210 is slidably connected to the middle of the connecting shell 27. A limiting block 29 is fixedly connected to the side wall of one end of the sliding plate 210. The outer wall of the limiting block 29 is slidably connected to the middle of the connecting shell 27. A limiting groove 8 is formed in the middle of the through groove 4. The limiting block 29 is inserted into the middle of the limiting groove 8. An L-shaped push plate 28 is fixedly connected to the side wall of the other end of the sliding plate 210. The outer wall of the L-shaped push plate 28 is slidably connected to the middle of the connecting plate 25. During operation, by inserting the limiting block 29... The middle part of the limiting groove 8 can limit the connecting plate 25 to ensure the stability of the reagent tube. When the connecting plate 25 is pushed, the L-shaped push plate 28 can be pushed to drive the slide plate 210 to move. The slide plate 210 will drive the limiting block 29 to move, so that it is disengaged from the middle part of the limiting groove 8. At this time, the connecting plate 25 can be pushed to move and adjust the position of the reagent tube. When no adjustment is needed, stop pressing the L-shaped push plate 28 and let the limiting block 29 insert into the middle part of the limiting groove 8.
[0020] Furthermore, such as Figure 4 and Figure 5As shown, a spring 211 is fixed to the side wall of the end of the slide plate 210, and the other end of the spring 211 is fixed to the middle of the connecting shell 27. Multiple sets of springs 211 are provided, and one end of each set of springs 211 is fixed to the side wall of the other end of the slide plate 210, and the other end of each set of springs 211 is fixed to the middle of the connecting plate 25. During operation, the springs 211 ensure that the limiting block 29 can be stably inserted into the middle of the limiting groove 8 when the L-shaped push plate 28 is not pressed, thus ensuring the stability of the limiting block 29 in limiting the connecting plate 25. The multiple sets of springs 211 ensure the uniformity of the elastic force on the slide plate 210.
[0021] Furthermore, such as Figure 2 and Figure 4 As shown, a protective plate 26 is fixed to the top of the connecting shell 27, and a sliding groove 7 is provided on the top side of the middle part of the through groove 4. The outer wall of the protective plate 26 is slidably connected to the middle part of the sliding groove 7. During operation, the through groove 4 can be sealed by the protective plate 26 to ensure the safety of the reagent tube in the middle of the storage box 1 and reduce the risk of the reagent tube being corroded by external bacteria.
[0022] Furthermore, such as Figure 1 - Figure 2 and Figure 4 As shown, multiple sets of cover plate 2, fixing block 21, round rod 22, guide plate 23, connecting plate 25, and protective plate 26 are provided, and these multiple sets of cover plate 2, fixing block 21, round rod 22, guide plate 23, connecting plate 25, and protective plate 26 are linearly and uniformly arranged on the side wall of storage box 1. The multiple sets of fixing blocks 21 are fixed to the top of partition 5. During operation, the multiple sets of blocks can individually seal the different areas separated by the multiple sets of partition 5, ensuring that the reagent tubes in different areas can be operated independently, thus improving practicality.
[0023] Furthermore, such as Figure 3 As shown, the top of the extrusion block 31 is set as an inclined surface. During operation, the inclined surface ensures that when the extrusion block 31 moves, the L-shaped push plate 28 will contact it and move along the inclined surface. Thus, when the push bar 3 contacts the connecting plate 25, under the action of the extrusion block 31, the L-shaped push plate 28 will cause the limiting block 29 to move out from the middle of the limiting groove 8, ensuring that the push bar 3 can smoothly push multiple sets of connecting plates 25 to move.
[0024] Working principle: The storage box 1 is divided into multiple sections by multiple sets of partitions 5 in the middle, and a set of fixing brackets 6 is set in the middle of each section. This allows different types of reagent tubes to be stored separately. When a reagent tube needs to be removed from the middle of the storage box 1, the L-shaped push plate 28 can be pushed to move the sliding plate 210. The sliding plate 210 will move the limiting block 29, causing it to disengage from the middle of the limiting groove 8. At this time, the connecting plate 25 can be pushed to move. The connecting plate 25 will move the fixing bracket 6 through the connecting shell 27. The fixing bracket 6 will move the reagent tube installed in the middle. At the same time, the connecting plate 25 will move the guide plate. 23 moves, and the outer wall of the round rod 22 slides in the middle of the V-groove 24 and moves along the slope of the V-groove 24. Since the cover plate 2 and the fixing block 21 are rotatably connected, when the round rod 22 moves, it will drive the cover plate 2 to rotate on the outer wall at the top of the fixing block 21. At the same time, the round rod 22 will rotate on the side wall of the cover plate 2 to adjust the movement and ensure the smooth cooperation between the structures. In this way, when the reagent tube is sent out from the middle of the storage box 1, the cover plate 2 will flip open at the same time, which improves convenience. The space for taking out the reagent tube that has moved out from the middle of the storage box 1 will be larger, and the staff can easily take out the reagent tube, which improves convenience.
[0025] When all the reagent tubes in the middle of storage box 1 need to be removed together, the rectangular plate 32 can be pushed to move. The rectangular plate 32 will simultaneously drive the pusher bar 3 and the squeezing block 31 to move. When the pusher bar 3 moves, it will drive the slider 33 to slide in the middle of the slide groove 9, ensuring the stability of the pusher bar 3's movement. In addition, when the squeezing block 31 moves, the L-shaped pusher plate 28 will contact it and move along the inclined surface until the pusher bar 3 contacts the multiple sets of connecting plates 25 at the same time. At this time, the limiting block 29 will move out from the middle of the limiting groove 8 under the action of the squeezing block 31. Then, continue pushing the rectangular plate 32. The rectangular plate 32 will push the connecting plate 25 to move through the pushing bar 3. The connecting plate 25 will drive the fixing frame 6 to move through the connecting shell 27. At the same time, the cover plate 2 will be flipped open through the guide plate 23. After the operation is completed, release the rectangular plate 32, so that it and the squeezing block 31 will fall down under gravity. At this time, the limiting block 29 will be reset under the elastic action of the spring 211 and inserted into the middle of the limiting groove 8. In this way, all the reagent tubes in the middle of the storage box 1 can be moved out from the middle of the storage box 1 at the same time. There is no need for the staff to push out different types of reagent tubes one by one, which improves convenience.
[0026] The above embodiments are only used to illustrate the technical solution of this utility model, and are not intended to limit it.
Claims
1. A multiplex PCR detection kit for pathogenic bacteria of dairy goat mastitis, comprising a storage box (1), characterized in that: The storage box (1) has multiple partitions (5) fixed in the middle. The storage box (1) has a fixed frame (6) slidably connected in the middle. The storage box (1) has a fixed block (21) fixed at the top. The outer wall of the top of the fixed block (21) is rotatably connected to a cover plate (2). The storage box (1) has a through groove (4) on its side wall. The through groove (4) has a connecting shell (27) slidably connected in the middle. The end of the connecting shell (27) is fixed to the side wall at the bottom of the fixed frame (6). The other end of the connecting shell (27) is fixed to a connecting plate (25). The top side of the end of the connecting plate (25) is fixed to a guide plate (23). The side wall at the top of the guide plate (23) has a V-shaped groove (24). The side wall of the cover plate (2) is rotatably connected to a round rod (22). The outer wall of the round rod (22) is slidably connected to the middle of the V-shaped groove (24).
2. The multiplex PCR detection kit for pathogenic bacteria of mastitis in dairy goats according to claim 1, characterized in that: The storage box (1) has a push bar (3) slidably connected to the bottom side of the connecting plate (25) on its side wall. A squeezing block (31) is slidably connected to the middle of the push bar (3). A rectangular plate (32) is fixed to the bottom of the squeezing block (31). A slider (33) is fixed to the side wall of the push bar (3). A second sliding groove (9) is opened on the side wall of the storage box (1). The outer wall of the slider (33) is slidably connected to the middle of the second sliding groove (9).
3. The multiplex PCR detection kit for pathogenic bacteria of mastitis in dairy goats according to claim 1, characterized in that: A sliding plate (210) is slidably connected to the middle of the connecting shell (27). A limiting block (29) is fixed to the side wall of the end of the sliding plate (210). The outer wall of the limiting block (29) is slidably connected to the middle of the connecting shell (27). A limiting groove (8) is opened in the middle of the through groove (4). The limiting block (29) is inserted into the middle of the limiting groove (8). An L-shaped push plate (28) is fixed to the side wall of the other end of the sliding plate (210). The outer wall of the L-shaped push plate (28) is slidably connected to the middle of the connecting plate (25).
4. The multiplex PCR detection kit for pathogenic bacteria of mastitis in dairy goats according to claim 3, characterized in that: A spring (211) is fixed to the side wall of the end of the slide plate (210), and the other end of the spring (211) is fixed to the middle of the connecting shell (27). There are multiple sets of springs (211), and one end of each set of springs (211) is fixed to the side wall of the other end of the slide plate (210), and the other end of each set of springs (211) is fixed to the middle of the connecting plate (25).
5. The multiplex PCR detection kit for pathogenic bacteria of mastitis in dairy goats according to claim 4, characterized in that: The top of the connecting shell (27) is fixed with a protective plate (26), and a sliding groove (7) is opened on the top side of the middle of the through groove (4). The outer wall of the protective plate (26) is slidably connected to the middle of the sliding groove (7).
6. The multiplex PCR detection kit for pathogenic bacteria of mastitis in dairy goats according to claim 5, characterized in that: The cover plate (2), fixing block (21), round rod (22), guide plate (23), connecting plate (25), and protective plate (26) are provided in multiple sets, and the multiple sets of cover plate (2), fixing block (21), round rod (22), guide plate (23), connecting plate (25), and protective plate (26) are linearly and uniformly arranged on the side wall of the storage box (1), and the multiple sets of fixing blocks (21) are fixed to the top of the partition (5).
7. The multiplex PCR detection kit for pathogenic bacteria of mastitis in dairy goats according to claim 2, characterized in that: The top of the extrusion block (31) is set as a slope.