A type of nucleic acid dye anti-volatile dropper bottle

Abstract

This utility model discloses a nucleic acid dye anti-volatile dropper bottle, specifically relating to the field of molecular biology experimental consumables technology. It includes a rubber base, an upper receiving mechanism, a first sealing mechanism, a second sealing mechanism, and a flow guiding mechanism in the middle of the receiving mechanism. The nucleic acid dye anti-volatile dropper bottle of this utility model, through the designed first sealing mechanism, combined with the fitting design of the rubber sealing ring and the second annular groove, fills the tiny gap between the bottle cap and the bottle mouth, preventing dye vapor from escaping from the outer periphery of the bottle mouth. The second annular groove precisely matches the size of the bottle body and the bottle mouth. The elastic material of the rubber sealing ring deforms as the threads are tightened, further enhancing the sealing performance. Without completely disassembling the bottle cap, only the first sealing mechanism needs to be unscrewed, and the dropper can be passed through the valve of the second sealing mechanism to obtain the liquid. This reduces operational steps and prevents direct contact between the dye inside the bottle and the outside air, reducing the probability of contamination.

Description

Technical Field

[0001] This utility model relates to the field of molecular biology experimental consumables technology, and in particular to a nucleic acid dye anti-volatile dropper bottle. Background Technology

[0002] In molecular biology research, nucleic acid electrophoresis is a core experimental technique for separating and identifying DNA and RNA, while nucleic acid dyes (such as ethidium bromide and SYBR Green) are key reagents for visualizing nucleic acids. These dyes are generally volatile and photosensitizing: on the one hand, most nucleic acid dye molecules contain volatile functional groups, and when stored at room temperature or when the dropper bottle is repeatedly opened to take liquid, the dye vapor can easily escape from the gaps at the bottle opening, which not only leads to a decrease in reagent concentration and poor reproducibility of experimental results, but the volatilization of some toxic dyes (such as ethidium bromide) can also harm the health of laboratory personnel. On the other hand, nucleic acid dyes are sensitive to ultraviolet and visible light, and long-term exposure can easily cause degradation, losing the ability to bind to nucleic acids, further shortening the lifespan of the reagents; Chinese Patent Publication No. CN218689592U discloses an anti-volatile reagent bottle, including a bottle body with an internal cavity. A bottle mouth is fixed to the bottle body, and a funnel is fixedly connected inside the bottle mouth. A bottle cap is detachably threaded to the outer periphery of the bottle mouth, and a stopper is rotatably connected inside the bottle cap. This invention, through the design of the funnel, can compress the air inside the funnel when the stopper is inserted, causing the liquid in the funnel part to be forced into the bottle body under air pressure. This creates a significant difference in liquid level between the funnel and the bottle body, allowing for visual inspection of the bottle's airtightness and reducing the area of ​​the reagent bottle's outlet, thereby reducing the evaporation process inside the bottle. The aforementioned patent documents still have the following defects in practice: During the liquid dispensing process, the bottle cap must be completely rotated off and the stopper pulled out before the reagent can be poured out through the funnel opening. During this process, the reagent inside the bottle will come into direct contact with the outside air, and the funnel opening, as the only outlet, remains open throughout the dispensing process. This can easily lead to a large amount of volatile reagent vapor escaping, resulting in a decrease in reagent concentration. Utility Model Content

[0003] The main purpose of this invention is to provide a nucleic acid dye anti-volatile dropper bottle, which can effectively solve the problems mentioned above.

[0004] To achieve the above objectives, the technical solution adopted by this utility model is as follows: A nucleic acid dye anti-volatile dropper includes a rubber base, an upper receiving mechanism, a first sealing mechanism, a second sealing mechanism, and a flow guiding mechanism in the middle of the receiving mechanism.

[0005] Preferably, the receiving mechanism includes a bottle body, which is disposed on the upper part of the rubber base. The bottle body has threads on the outside of the bottle mouth, and the bottle body has an annular groove in the inner cavity of the bottle mouth.

[0006] Preferably, the upper plane of the bottle body is higher on the inside and lower on the outside.

[0007] Preferably, the flow guiding mechanism includes a flow guiding plate, which is disposed on the upper part of the bottle body, and a circular groove is provided in the middle of the flow guiding plate.

[0008] Preferably, the upper surface of the guide plate is lower on the inside and higher on the outside, and the lower surface of the guide plate is higher on the inside and lower on the outside.

[0009] Preferably, the sealing mechanism includes a plastic cap, which is disposed on the outside of the bottle opening. A rubber sealing ring is provided on the inner upper surface of the plastic cap, a sealing plug is provided in the middle of the rubber sealing ring, and an annular groove is provided at the lower part of the rubber sealing ring.

[0010] Preferably, the size of the second annular groove matches the size of the bottle body and the bottle opening.

[0011] Preferably, the second sealing mechanism includes an arc-shaped block, which is disposed in an annular groove. The arc-shaped block has a circular groove in the middle, and three valves are disposed inside the circular groove.

[0012] Compared with the prior art, the present invention has the following beneficial effects: This device utilizes a specially designed sealing mechanism. The plastic cap is tightly connected to the bottle body via threads. Combined with the fitting design of the rubber sealing ring and the annular groove, it can fill the tiny gaps between the cap and the bottle mouth, preventing dye vapor from escaping from the outer periphery of the bottle mouth. The annular groove precisely matches the size of the bottle body and the bottle mouth. The elastic material of the rubber sealing ring deforms as the threads are tightened, further enhancing the sealing performance. There is no need to completely disassemble the bottle cap; simply unscrew the sealing mechanism and pass the dropper through the valve of the sealing mechanism to obtain the liquid. This reduces the number of operation steps and prevents the dye inside the bottle from directly contacting the outside air, thus reducing the probability of contamination. This device, through its designed sealing mechanism two, works in conjunction with sealing mechanism one to construct a dual protection system of "external leak prevention + internal dynamic sealing." The three valves of sealing mechanism two are tightly closed in their natural state, forming a core sealing barrier inside the bottle. They only open and adhere to the tube wall when the dropper is inserted, preventing significant evaporation caused by keeping the bottle opening open throughout the dispensing process. Compared to traditional dropper bottles, this design reduces the evaporation rate of nucleic acid dyes, effectively maintaining stable reagent concentrations and ensuring the reproducibility of experimental results. The annular groove one provides stable installation space for the arc-shaped block, ensuring that the valves are always centered at the bottle opening, preventing seal failure due to installation misalignment, and strengthening the anti-evaporation effect through structural details. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a cross-sectional view of the overall structure of this utility model; Figure 3 This is a front view of a partial sectional structure of the present invention; Figure 4 This is a schematic diagram of the sealing mechanism of this utility model; Figure 5 This is a schematic diagram of the sealing mechanism of this utility model.

[0014] In the diagram: 1. Rubber base; 2. Receiving mechanism; 21. Bottle body; 22. Thread; 23. Annular groove one; 3. Sealing mechanism one; 31. Plastic cap; 32. Rubber sealing ring; 33. Sealing plug; 34. Annular groove two; 4. Sealing mechanism two; 41. Arc-shaped block; 42. Circular groove one; 43. Valve; 5. Flow guiding mechanism; 51. Flow guiding plate; 52. Circular groove two. Detailed Implementation

[0015] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0016] Example 1, as Figure 1 - Figure 2 As shown, a nucleic acid dye anti-volatile dropper includes a rubber base 1, a receiving mechanism 2 on the upper part of the rubber base 1, a sealing mechanism 1 3 on the upper part of the receiving mechanism 2, a sealing mechanism 2 4 on the upper part of the receiving mechanism 2, and a flow guiding mechanism 5 in the middle of the receiving mechanism 2. In this embodiment, the rubber base 1 can prevent the receiving mechanism 2 from sliding on the experimental table surface and maintain the stability of the receiving mechanism 2. When taking liquid, unscrew the sealing mechanism 3 and insert the dropper into the receiving mechanism 2. The dropper extends into the receiving mechanism 2 through the sealing mechanism 4 and then through the flow guiding mechanism 5 to take out the dye in the receiving mechanism 2. For preservation of nucleic acid dyes, see [link / reference]. Figure 3 The receiving mechanism 2 includes a bottle body 21, which is set on the upper part of the rubber base 1. The bottle body 21 has a thread 22 on the outside of the bottle mouth, and an annular groove 23 in the inner cavity of the bottle mouth. The upper plane of the bottle body 21 is higher on the inside and lower on the outside. During implementation, the bottle body 21 is made of brown borosilicate glass. The brown color can block more than 90% of ultraviolet and visible light, preventing the degradation of photosensitive nucleic acid dyes. Borosilicate glass is chemically inert and does not react with nucleic acid dyes. It is also resistant to rapid cooling and heating, and can be switched between -20℃ refrigeration and 37℃ room temperature without cracking. The thread 22 connects and fixes the bottle body 21 to the plastic cap 31. Together with the sealing mechanism 1 3, it improves the sealing effect of the bottle mouth of the bottle body 21 and reduces the evaporation of dye vapor from the gaps in the bottle mouth of the bottle body 21. At the same time, the thread 22 connection facilitates the disassembly and installation of the plastic cap 31, making it convenient to use dye and clean and maintain the dropper bottle. The annular groove 1 23 provides installation and positioning space for the sealing mechanism 2 4, ensuring that the arc block 41 can be stably fixed in the inner cavity of the bottle mouth, making it convenient to install and remove the arc block 41. To seal the housing mechanism 2, see [reference needed]. Figure 4 The sealing mechanism 3 includes a plastic cap 31, which is located on the outside of the bottle mouth of the bottle body 21. A rubber sealing ring 32 is provided on the inner upper surface of the plastic cap 31. A sealing plug 33 is provided in the middle of the rubber sealing ring 32. An annular groove 34 is provided at the lower part of the rubber sealing ring 32. The size of the annular groove 34 matches the size of the bottle mouth of the bottle body 21. During implementation, the plastic cap 31 is connected to the bottle body 21 by the thread 22, achieving a preliminary seal at the bottle mouth of the bottle body 21. The rubber sealing ring 32 and the annular groove 34 cooperate to clamp between the plastic cap and the bottle mouth, filling the gap between them and effectively preventing dye vapor from escaping from the connection gap between the plastic cap and the bottle body, thus significantly reducing the evaporation rate. The annular groove 34 and the rubber sealing ring 32 are integrated and fit into the bottle mouth of the bottle body 21, always maintaining a tight fit with the bottle mouth, further improving the sealing effect.

[0017] Example 2: To reduce the volatilization of nucleic acid dyes during liquid collection, refer to... Figure 5 The sealing mechanism 2 4 includes an arc-shaped block 41, which is disposed in an annular groove 23. A circular groove 42 is provided in the middle of the arc-shaped block 41, and three valves 43 are provided inside the circular groove 42. During implementation, the arc-shaped block 41 is made of polytetrafluoroethylene, which is chemically inert, does not react with any nucleic acid dyes, does not adsorb dyes, and is resistant to high temperatures and wear. The arc-shaped block 41 is installed in the annular groove 23, and the central circular groove 42 provides a channel for the dropper and also assists in sealing. The valve 43 is elastic and is set inside the circular groove 42. There are three valves in total, which are centrally symmetrically distributed. In its natural state, they are tightly closed to form a seal. When the dropper is inserted, the valve 43 can be smoothly opened and tightly attached to the outer wall of the dropper to achieve dynamic sealing and reduce the leakage of dye vapor during use. After the dropper is pulled out, the valve 43 quickly rebounds and closes to prevent air from entering the bottle and causing dye oxidation and degradation, while also preventing dye vapor from overflowing. For further details, please refer to [link / reference]. Figure 3The flow guiding mechanism 5 includes a flow guiding plate 51, which is disposed on the upper part of the bottle body 21. A circular groove 52 is provided in the middle of the flow guiding plate 51. The upper surface of the flow guiding plate 51 is lower on the inside and higher on the outside, and the lower surface of the flow guiding plate 51 is higher on the inside and lower on the outside. During implementation, the guide plate 51 is also made of brown borosilicate glass and is set on the upper part of the bottle body 21. It guides the flow of dye in the bottle body 21. When adding and cleaning the bottle body 21, the guide plate 51 can guide the dye to converge towards the center, avoiding liquid residue in the bottle body 21. The circular groove 52 allows the dropper to pass through, which can also prevent liquid from splashing to the sealing mechanism 3 when taking liquid, thereby reducing the volatilization of dye.

[0018] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid dye anti-evaporation dropper bottle comprising a rubber base (1), characterized in that: The rubber base (1) is provided with a receiving mechanism (2) on the upper part, a sealing mechanism one (3) is provided on the upper part of the receiving mechanism (2), a sealing mechanism two (4) is provided on the upper part of the receiving mechanism (2), and a flow guiding mechanism (5) is provided in the middle of the receiving mechanism (2). The receiving mechanism (2) includes a bottle body (21), which is set on the upper part of the rubber base (1). The bottle body (21) has a thread (22) on the outside of the bottle mouth, and an annular groove (23) is provided in the inner cavity of the bottle mouth. The upper part of the bottle body (21) has a higher inner surface and a lower outer surface. The sealing mechanism (3) includes a plastic cap (31), which is located outside the bottle mouth of the bottle body (21). A rubber sealing ring (32) is provided on the inner wall of the plastic cap (31), and a sealing plug (33) is provided in the middle of the rubber sealing ring (32). An annular groove (34) is provided at the lower part of the rubber sealing ring (32). The sealing mechanism 2 (4) includes an arc-shaped block (41), which is disposed in an annular groove 1 (23). A circular groove 1 (42) is provided in the middle of the arc-shaped block (41), and three valves (43) are provided inside the circular groove 1 (42).

2. The nucleic acid dye evaporation resistant dropper bottle of claim 1, wherein: The flow guiding mechanism (5) includes a flow guiding plate (51), which is disposed on the upper part of the bottle body (21), and a circular groove (52) is provided in the middle of the flow guiding plate (51).

3. The nucleic acid dye evaporation resistant droplet bottle of claim 2, wherein: The upper surface of the guide plate (51) is lower on the inside and higher on the outside, and the lower surface of the guide plate (51) is higher on the inside and lower on the outside.

4. The nucleic acid dye evaporation resistant droplet bottle of claim 1, wherein: The size of the annular groove 2 (34) matches the size of the bottle mouth of the bottle body (21).

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