A magnetic frame unit and a magnetic frame assembly

Abstract

This application discloses a magnetic rack unit and magnetic rack assembly, including a base component, a magnetic suction component, and a swinging component. The base component has a first sleeve, and the magnetic suction component is disposed on the base component. The swinging component has a second sleeve and a socket for installing a reagent tube. A first pin can pass through the first and second sleeves, and the first pin is tightly engaged with the first and second sleeves. Under the action of an external force, the swinging component can rotate around the first pin to move the reagent tube closer to or away from the magnetic suction component. When the external force is removed, the swinging component is fixed in position under the frictional force of the base component and / or the first pin. The magnetic rack unit provided by this application can achieve stepless adjustment when adjusting the relative angle between the swinging component and the base component. Continuous adjustment of the magnetic force can be achieved through simple operation to meet the needs of DNA separation and extraction with different contents. It is highly feasible, widely applicable, and easy to operate, effectively improving the efficiency of DNA separation and extraction, and reducing time and equipment costs.

Description

Technical Field

[0001] This utility model relates to the field of magnetic separation technology, specifically to a magnetic frame monomer and magnetic frame assembly for magnetic separation during DNA extraction from biological samples. Background Technology

[0002] In molecular biology, clinical medicine, forensic genetics, and other fields, DNA extraction is fundamental to PCR amplification, sequencing, and other operations, and its quality directly affects the accuracy and stability of downstream analyses. DNA extraction methods mainly include column extraction, organic reagent extraction, and magnetic bead-based DNA extraction. Compared to traditional column or organic reagent extraction methods, magnetic bead-based DNA extraction technology has become increasingly popular and is one of the most mainstream methods due to its ease of operation, automation, and suitability for high-throughput processing. The key to magnetic bead extraction lies in the control of magnetic force and precise adjustment of its position to ensure that the magnetic beads are effectively adsorbed onto the inner wall of the reaction vessel without losing the target DNA, and to facilitate subsequent elution.

[0003] However, the magnetic force of existing magnetic racks is not adjustable. Different applications have different requirements for magnetic force strength, and a fixed magnetic force may lead to insufficient adsorption or excessive concentration of magnetic beads. Especially in some trace reactions, users need to control the aggregation position of magnetic beads, which existing equipment cannot meet, affecting separation efficiency. This results in poor versatility, requiring customized magnetic racks for DNA extraction operations with different magnetic forces.

[0004] Therefore, designing a magnetic rack with adjustable magnetic force for adsorption inside PCR tubes has clear practical value.

[0005] In view of the above, this utility model is hereby proposed. Utility Model Content

[0006] To solve one of the above-mentioned technical problems, this utility model provides a magnetic frame unit and a magnetic frame assembly.

[0007] The primary objective of this application is to provide a magnetic frame unit, which offers the following technical solution:

[0008] A magnetic frame unit, comprising:

[0009] A base component, the base component having a first sleeve;

[0010] A magnetic attraction component is disposed on the base component;

[0011] A swinging component has a second sleeve and a socket for installing a reagent tube. A first pin can pass through the first sleeve and the second sleeve to connect the swinging component and the base component. The first pin is in close cooperation with the first sleeve and the second sleeve.

[0012] Under the action of external force, the swinging component can rotate around the first pin to make the reagent tube move closer to or away from the magnetic attraction component;

[0013] When the external force is removed, the swinging member is fixed in position under the frictional force of the base member and / or the first pin.

[0014] Preferably, the base component has a base and a longitudinal body;

[0015] The longitudinal body is vertically connected to the base, and the magnetic suction component is disposed on the longitudinal body;

[0016] The first sleeve is disposed at the end of the longitudinal body opposite to the base;

[0017] Under the action of external force, the oscillating component can rotate around the first pin to adjust the angle with the longitudinal body.

[0018] Preferably, a clearance space is provided at the end of the longitudinal body that is away from the base;

[0019] The clearance space and the first sleeve are arranged sequentially along the length of the base component;

[0020] When the swing member and the base member are connected, the second sleeve is located within the clearance space.

[0021] Preferably, the magnetic frame unit includes two oscillating elements;

[0022] The base component has two first sleeves;

[0023] The two first sleeves are respectively disposed on both sides of the longitudinal body along the thickness direction;

[0024] The two swinging parts are respectively connected to the corresponding first sleeves via the first pin.

[0025] Preferably, a mounting groove is provided on the longitudinal body;

[0026] The magnetic component is embedded in the mounting slot.

[0027] Preferably, the longitudinal body includes a first longitudinal segment and a second longitudinal segment;

[0028] The first longitudinal segment is connected to the base, and the magnetic attraction component is provided on the first longitudinal segment;

[0029] The second longitudinal segment is connected to the end of the first longitudinal segment that is away from the base;

[0030] The thickness of the second longitudinal segment gradually decreases in the direction from the base to the first longitudinal segment;

[0031] The first sleeve is disposed at one end of the second longitudinal section opposite to the first longitudinal section.

[0032] Preferably, the swing member is provided with at least two insertion holes, and the insertion holes are arranged sequentially along the rotation axis of the swing member;

[0033] At least two magnetic attraction components are provided on the longitudinal body, and each magnetic attraction component is arranged sequentially along the rotation axis of the swinging component;

[0034] As the swinging component rotates around the first pin, each socket on the swinging component moves closer to or further away from the corresponding magnetic component.

[0035] Preferably, the base component is provided with a through groove, which is parallel to the first pin.

[0036] Preferably, the reagent tube includes a conical section and a straight section;

[0037] The straight section is connected to the wide end of the tapered section, and a flange is provided at the end of the straight section away from the tapered section. The outer diameter of the flange is larger than the inner diameter of the insertion hole.

[0038] With the reagent tube installed on the swinging member, the straight section extends through the insertion hole.

[0039] The second objective of this application is to provide a magnetic frame assembly, which provides the following technical solution:

[0040] A magnetic rack assembly, comprising:

[0041] Multiple magnetic frame units as described above, each of the magnetic frame units being arranged sequentially along the length direction;

[0042] The second pin passes through the base component of each of the magnetic frame units to connect and fix the base components of each magnetic frame unit.

[0043] By adopting the above technical solution, this application has the following beneficial effects:

[0044] The magnetic rack unit provided in this application allows for stepless adjustment of the relative angle between the oscillating component and the substrate component. Continuous adjustment of the magnetic force is achieved through simple operation to meet the needs of DNA separation and extraction with varying concentrations. This approach is highly feasible, widely applicable, and easy to operate, effectively improving the efficiency of DNA separation and extraction while reducing time and equipment costs. Attached Figure Description

[0045] The accompanying drawings, which form part of this application, are used to provide a further understanding of the present invention. The illustrative embodiments and descriptions of the present invention are used to explain the present invention, but do not constitute an undue limitation of the present invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without any creative effort.

[0046] Figure 1 This diagram shows a structural schematic of the magnetic frame unit provided in Embodiment 1 of the present invention;

[0047] Figure 2 This diagram shows the structural schematic of the base component of the magnetic frame unit provided in Embodiment 1 of this utility model;

[0048] Figure 3 This diagram shows the structural schematic of the swing component of the magnetic frame unit provided in Embodiment 1 of this utility model;

[0049] Figure 4 This diagram shows the structure of the reagent tube of the magnetic rack unit provided in Embodiment 1 of this utility model;

[0050] Figure 5 This diagram shows the structure of the first pin of the magnetic frame unit provided in Embodiment 1 of this utility model;

[0051] Figure 6 This diagram shows the structure of the magnetic attraction component of the magnetic frame unit provided in Embodiment 1 of this utility model;

[0052] Figure 7 This diagram shows the structure of the second pin of the magnetic frame unit provided in Embodiment 1 of this utility model;

[0053] Figure 8 This diagram shows a structural schematic of the magnetic frame assembly provided in Embodiment 2 of this utility model;

[0054] Figure 9 This diagram illustrates the assembly process between the base components of each magnetic frame unit during the assembly of the magnetic frame assembly provided in Embodiment 2 of this utility model.

[0055] Figure 10 This is a schematic diagram showing the process of installing the oscillating component onto each base component using the magnetic frame assembly provided in Embodiment 2 of this utility model;

[0056] Figure 11 This diagram shows a structure in which the swinging members on both sides of the magnetic frame assembly provided in Embodiment 2 of this utility model swing to a horizontal position.

[0057] Figure 12This diagram illustrates the structure of the magnetic frame assembly provided in Embodiment 2 of the present invention, where the swinging members on both sides are at acute angles to the longitudinal body.

[0058] Figure 13 This diagram illustrates the structure of the magnetic frame assembly provided in Embodiment 2 of this utility model, showing the different angles between the swinging members on both sides and the longitudinal body.

[0059] In the figure: base component 1, base 11, first sleeve 111, longitudinal body 12, first longitudinal section 121, second longitudinal section 122, through groove 13, clearance space 14, mounting groove 15, magnetic suction component 2, swing component 3, second sleeve 31, insertion hole 32, first pin 4, first shaft 41, first cap 42, reagent tube 5, conical section 51, straight section 52, flange 53, magnetic frame unit 100, second pin 200, second shaft 201, second cap 202. Detailed Implementation

[0060] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0061] In the description of this utility model, it should be noted that the terms "upper", "lower", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0062] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0063] Example 1

[0064] like Figures 1 to 13As shown in the illustration, this application provides a magnetic frame unit, including a base component 1, a magnetic attraction component 2, and a swing component 3. The base component 1 has a first sleeve 111, and the magnetic attraction component 2 is disposed on the base component 1. The swing component 3 has a second sleeve 31 and a insertion hole 32. The insertion hole 32 is used to install a reagent tube 5. A first pin 4 can pass through the first sleeve 111 and the second sleeve 31, connecting the swing component 3 and the base component 1. The first pin 4 is tightly engaged with the first sleeve 111 and the second sleeve 31. Under the action of an external force, the swing component 3 can rotate around the first pin 4 to move the reagent tube 5 closer to or away from the magnetic attraction component 2. When the external force is removed, the swing component 3 is fixed in position under the frictional force of the base component 1 and / or the first pin 4.

[0065] The magnetic rack unit provided in this application has a magnetic suction component 2 disposed on a base component 1, and a swinging component 3 disposed on the base component 1. In use, the reagent tube 5 is inserted into the insertion hole 32 on the swinging component 3. The swinging component 3 can drive the reagent tube 5 to swing toward the magnetic suction component 2 or swing away from the magnetic suction component 2, thereby adjusting the distance between the reagent tube 5 and the magnetic suction component 2, and thus adjusting the magnetic force on the reagent inside the reagent tube 5 during the DNA extraction process.

[0066] Because the substrate 1 and the oscillating component 3 are connected by a first pin 4 that is slightly interference-fitted into the first sleeve 111 and the second sleeve 31, stepless adjustment can be achieved when adjusting the relative angle between the oscillating component 3 and the substrate 1. This simple operation allows for continuous adjustment of the magnetic force to meet the needs of DNA separation and extraction at different concentrations. It is highly feasible, widely applicable, and easy to operate, effectively improving the efficiency of DNA separation and extraction while reducing time and equipment costs.

[0067] The first pin 4, the first sleeve 111, and the second sleeve 31 all have a certain interference fit. When the first pin 4 is inserted into the first sleeve 111 and the second sleeve 31, the base component and the swing component 3 are tightly fitted. Therefore, without a large external force, the swing component 3 can remain at any angle relative to the base component and will not change its position due to its own weight or the weight of the reagent tube 5. If the swing component 3 is oscillated for a long time, causing the fit between the first pin 4 and the first sleeve 111 and the second sleeve 31, or between the base component and the swing component 3, to become less tight, a tight fit can be restored by replacing the first pin 4, replacing it with a thicker first pin 4, or tightening the base component and the swing component 3, thus ensuring the stability of the position of the swing component 3 and the base component 1.

[0068] In some possible implementations, the base component 1 has a base 11 and a longitudinal body 12. The longitudinal body 12 is vertically connected to the base 11, and the magnetic suction component 2 is disposed on the longitudinal body 12. The first sleeve 111 is disposed at the end of the longitudinal body 12 opposite to the base 11. Under the action of external force, the swinging component 3 can rotate around the first pin 4 to adjust the angle with the longitudinal body 12, thereby adjusting the distance between the reagent tube 5 and the magnetic suction component 2. The width of the base 11 is greater than the width of the longitudinal body 12 of the base 11, which ensures that the magnetic frame unit 100 can be placed stably on a support surface such as a desktop or laboratory table, and is not easy to tip over. The height of the longitudinal body 12 is greater than the width of the swinging component 3, providing space for the swinging component 3 to swing, so that there is no interference between the swinging component 3 and the base 11.

[0069] In some possible implementations, a clearance space 14 is provided at the end of the longitudinal body 12 opposite to the base 11, and the clearance space 14 and the first sleeve 111 are arranged sequentially along the length direction of the base component 1. When the swinging component 3 is connected to the base component 1, the second sleeve 31 is located within the clearance space 14. Providing the clearance space 14 and placing the second sleeve 31 within it ensures that the first sleeve 111 and the second sleeve 31 are on the same straight line, making installation more convenient and preventing the swinging of the swinging component 3 from being restricted by other structures.

[0070] The clearance space 14 has the same length as the second sleeve 31. The second sleeve 31 can be completely set in the clearance space 14 without protruding. This will not affect the connection between adjacent magnetic frame units 100, and will also ensure that the swinging part 3 and the base part 1 are tightly fitted together after installation to generate a large friction force.

[0071] Furthermore, the first sleeve 111 and the second sleeve 31 are of the same length, so that the base component 1 and the swing component 3 are connected to the first pin 4 with the same stability, and it is not easy for either side to slip.

[0072] In some possible implementations, the magnetic rack unit 100 includes two swinging members 3. The base component 1 has two first sleeves 111, which are respectively disposed on both sides of the longitudinal body 12 along the thickness direction. The two swinging members 3 are respectively connected to the corresponding first sleeves 111 via first pins 4. The base component 1 may be "T"-shaped. The two swinging members 3, disposed on both sides of the longitudinal body 12, can increase the number of reagent tubes 5 that can be set in one magnetic rack unit 100, saving space and facilitating operation, carrying, and transportation. Furthermore, the swinging members 3 on both sides can swing at different angles, thereby extracting DNA of different concentrations.

[0073] In some possible implementations, a mounting groove 15 is provided on the longitudinal body 12, and the magnetic suction component 2 is embedded in the mounting groove 15. Installing the magnetic suction component 2 in the mounting groove 15 ensures stable installation of the magnetic suction component 2.

[0074] The longitudinal body 12 may have mounting grooves 15 on both sides, with magnetic components 2 installed in each groove 15. Alternatively, a through groove 13 may be provided in the middle of the longitudinal body 12, with the magnetic components 2 installed in the through groove 13, and both ends of the magnetic components 2 can release magnetic force outward.

[0075] In some possible implementations, the longitudinal body 12 includes a first longitudinal segment 121 and a second longitudinal segment 122. The first longitudinal segment 121 is connected to the base 11, and the magnetic attraction component 2 is disposed on the first longitudinal segment 121. The second longitudinal segment 122 is connected to the end of the first longitudinal segment 121 opposite to the base 11. The thickness of the second longitudinal segment 122 gradually decreases in the direction from the base 11 to the first longitudinal segment 121, and the first sleeve 111 is disposed at the end of the second longitudinal segment 122 opposite to the first longitudinal segment 121. The second longitudinal segment 122 is tapered, with a thinner side near the connection point with the swing member 3 and a thicker side, forming a slope that can precisely avoid the swing member 3, allowing the swing member 3 to have a larger swing space, and thus providing a larger adjustment range for the magnetic force.

[0076] In some possible implementations, the swing member 3 is provided with at least two insertion holes 32, and each insertion hole 32 is arranged sequentially along the rotation axis of the swing member 3. The longitudinal body 12 is provided with at least two magnetic suction components 2, and each magnetic suction component 2 is arranged sequentially along the rotation axis of the swing member 3. During the rotation of the swing member 3 around the first pin 4, each insertion hole 32 on the swing member 3 moves closer to or further away from the corresponding magnetic suction component 2. To ensure that the magnetic rack unit 100 is stable and not easily tipped over when placed on a table, lab bench, or other supporting surface, the magnetic rack unit 100 needs to have a certain length. Since the number of samples placed in each reagent tube 5 during DNA extraction is not large, and the volume of the reagent tube 5 is also small, a single magnetic rack unit 100 can be provided with more insertion holes 32, thereby increasing the number of reagent tubes 5 that can be placed in a single magnetic rack unit 100, further saving space, and further facilitating operation, carrying, and transportation.

[0077] In some possible implementations, a through groove 13 is provided on the base component 1, the through groove 13 being parallel to the first pin 4. The through groove 13 is used when assembling multiple magnetic frame units 100 together, with the second pin 200 sequentially penetrating each magnetic frame unit 100, thereby assembling multiple magnetic frame units 100 into a magnetic frame assembly.

[0078] In some possible implementations, such as Figure 4 As shown, the reagent tube 5 includes a conical section 51 and a straight section 52. The straight section 52 is connected to the wide end of the conical section 51. A flange 53 is provided at the end of the straight section 52 facing away from the conical section 51. The outer diameter of the flange 53 is larger than the inner diameter of the insertion hole 32. When the reagent tube 5 is installed on the swing member 3, the straight section 52 penetrates the insertion hole 32, and the flange 53 is positioned on the side of the swing member 3 facing away from the longitudinal body 12. The conical section 51 acts as a guide when the reagent tube 5 is inserted into the insertion hole 32, and the flange 53 prevents the reagent tube 5 from passing through the insertion hole 32. This structure makes it easier to install or remove the reagent tube 5 from the swing member 3.

[0079] Among them, reagent tube 5 can be an EP tube or a PCR tube, etc.

[0080] Example 2

[0081] like Figures 1 to 13 As shown, this embodiment provides a magnetic frame assembly, including multiple magnetic frame units 100 as described in Embodiment 1 and a second pin 200. The magnetic frame units 100 are arranged sequentially along their length, and the second pin 200 passes through the base component 1 of each magnetic frame unit 100 to connect and fix the base component 1 of each magnetic frame unit 100. The magnetic frame assembly provided in this embodiment is assembled from multiple magnetic frame units 100 as described in Embodiment 1. Using a modular construction approach, the number of magnetic frame units 100 and cylindrical magnets can be flexibly selected according to actual needs, enabling DNA separation and extraction from different numbers of reagent tubes 5 through a single device. This achieves magnetic separation and extraction of DNA with varying amounts, customized to demand, with high feasibility, wide applicability, and simple operation, effectively improving the efficiency of DNA separation and extraction while reducing time and cost.

[0082] The second pin 200 passes through the through slot 13 of each magnetic frame unit 100 in sequence with interference fit, so that multiple magnetic frame units 100 are stably formed into an integral magnetic frame assembly, which has high stability, is not easy to tip over, and is easy to move and carry.

[0083] In some possible implementations, the first pin 4 includes a first shaft 41 and a first cap 42, the first cap 42 being disposed at one end of the first shaft 41, and the main body diameter of the first cap 42 being larger than the diameter of the first shaft 41. The second pin 200 includes a second shaft 201 and a second cap 202, the second cap 202 being disposed at one end of the second shaft 201, and the main body diameter of the second cap 202 being larger than the diameter of the second shaft 201.

[0084] The preferred embodiments disclosed above are merely illustrative of this application. The preferred embodiments do not exhaustively describe modifications and variations. These embodiments are selected and specifically described in this specification to better explain the principles and practical applications of this application, thereby enabling those skilled in the art to better understand and utilize this application. This application is limited only by the claims and their full scope and equivalents.

Claims

1. A magnetic stand monomer, characterized by, The application relates to a magnetic frame, which comprises a base body provided with a first sleeve, a magnetic component, a swing body provided with a second sleeve and a hole for mounting a reagent tube, and a first pin shaft penetrating through the first sleeve and the second sleeve to connect the swing body and the base body. The swing body can rotate around the first pin shaft under external force to make the reagent tube close to or away from the magnetic component. The swing body is fixed in position under the friction of the base body and / or the first pin shaft when the external force is removed. The base body is provided with a base and a longitudinal body. The longitudinal body is vertically connected to the base, and the magnetic component is arranged on the longitudinal body. The first sleeve is arranged on one end of the longitudinal body away from the base.

2. The magnetic stand monomer of claim 1, wherein, The swing body can rotate around the first pin shaft under external force to adjust the included angle of the longitudinal body. The longitudinal body is provided with a clearance space at one end away from the base. The clearance space and the first sleeve are sequentially arranged along the length direction of the base body. The second sleeve is located in the clearance space when the swing body is connected to the base body.

3. The magnetic mount of claim 2, wherein, The base body is provided with two first sleeves. The two first sleeves are arranged on the two sides of the longitudinal body along the thickness direction. The two swing bodies are connected to the corresponding first sleeves through the first pin shaft respectively.

4. The magnetic stand monomer of claim 2, wherein, The longitudinal body is provided with a mounting groove. The magnetic component is embedded in the mounting groove. The longitudinal body comprises a first longitudinal section and a second longitudinal section. The first longitudinal section is connected to the base, and the magnetic component is arranged on the first longitudinal section.

5. The magnetic mount of claim 2, wherein, The second longitudinal section is connected to one end of the first longitudinal section away from the base. The thickness of the second longitudinal section gradually decreases in the direction from the base to the first longitudinal section.

6. The magnetic stand monomer of claim 2, wherein, The first sleeve is arranged on one end of the second longitudinal section away from the first longitudinal section. The swing body is provided with at least two holes, and the holes are sequentially arranged along the rotation axis of the swing body. The longitudinal body is provided with at least two magnetic components, and the magnetic components are sequentially arranged along the rotation axis of the swing body. Each hole of the swing body is close to or away from the corresponding magnetic component in the process of the swing body rotating around the first pin shaft. The base body is provided with a through groove parallel to the first pin shaft.

7. The magnetic mount of claim 2, wherein, The reagent tube comprises a conical section and a straight section. The straight section is connected to the wide end of the conical section, and a flange is arranged on one end of the straight section away from the conical section. The outer diameter of the flange is larger than the inner diameter of the hole.

8. The magnetic stand monomer of claim 1, wherein, The straight section penetrates through the hole when the reagent tube is mounted on the swing body.

9. The magnetic stand unit according to any one of claims 1 to 8, wherein The application relates to a magnetic frame, which comprises a base body provided with a first sleeve, a magnetic component, a swing body provided with a second sleeve and a hole for mounting a reagent tube, and a first pin shaft penetrating through the first sleeve and the second sleeve to connect the swing body and the base body. ​ ​ 10. A magnetic stand assembly, characterized by, ​ ​ ​

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