A miniature ex vivo placental tissue cell extraction device

Abstract

This utility model relates to a tissue and cell extraction device, and more particularly to a miniature ex vivo placental tissue and cell extraction device, comprising an operating box and a control terminal. Inside the operating box, from top to bottom, are a peeling and cutting mechanism, a filtering assembly, and a manifold. An extension tube is connected to the bottom of the manifold, and the other end of the extension tube passes through the side wall of the operating box and is connected to a collection bucket. A connecting valve is provided between the extension tube and the collection bucket. This utility model integrates the peeling and cutting mechanism, the filtering assembly, and the manifold inside the operating box, thereby integrating the functions of placental tissue peeling and cutting, filtering and separation, manifold collection, and external transport into one unit. The control terminal coordinates and controls the operation of each component, making the entire cell extraction process continuous and efficient, significantly shortening the cell extraction processing time for a single placental tissue, improving operational efficiency, and meeting the needs of large-scale target cell extraction.

Description

Technical Field

[0001] This utility model relates to a tissue cell extraction device, and more particularly to a miniature ex vivo placental tissue cell extraction device. Background Technology

[0002] In the field of biomedical engineering, tissue cell extraction devices are core equipment used to separate and purify target cells from isolated tissues. They are widely used in stem cell research, regenerative medicine, and clinical diagnostics. The core function of this equipment is to mechanically shear and break down tissue cells, then filter and separate the resulting mixture, collecting and purifying the cell suspension to obtain a high-purity, highly active cell suspension. This provides the basic raw material for subsequent culture, testing, or clinical applications.

[0003] Traditional tissue cell extraction devices are mostly open structures or have poor sealing performance, making them susceptible to invasion by external microorganisms during placental tissue washing and shearing operations. Furthermore, their large size prevents them from being placed in a bioreactor for extraction. Hematopoietic cells extracted from the placenta require extremely sterile environments; if they are contaminated by external sources during extraction, the purity of the extracted cells is directly affected, hindering subsequent applications. Existing devices require manual operation in the washing, shearing, filtering, and collection steps during target cell extraction, leading to numerous interventions, increased potential errors, and prolonged processing time for individual placental target cell extractions. This inefficiency makes it difficult to meet the needs of large-scale target cell extraction and processing. Utility Model Content

[0004] The purpose of this invention is to provide a miniature ex vivo placental tissue cell extraction device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A miniature ex vivo placental tissue cell extraction device includes an operation box and a control terminal. The operation box is equipped with a peeling and cutting mechanism, a filter assembly and a manifold from top to bottom. An extension tube is connected to the bottom of the manifold. The other end of the extension tube passes through the side wall of the operation box and is connected to a collection bucket. A connecting valve is provided between the operation box and the collection bucket on the extension tube. The collection tank is equipped with a collection valve on one side, which is connected to the inside of the collection tank. A pressure pump and a liquid level sensor are installed on the top surface of the collection tank. The control terminal is located outside the operating box and is electrically connected to the stripping and cutting mechanism, pressure pump, liquid level sensor, connecting valve, and acquisition valve.

[0006] Preferably, the stripping and cutting mechanism includes a cutting motor fixedly installed on the top wall inside the operating box. The bottom output end of the cutting motor is driven and connected to a transmission rod. The end of the transmission rod is connected to a support frame through a bearing. The two ends of the support frame are fixedly connected to the left and right side walls inside the operating box, respectively. The transmission rod is driven and connected to a movable disc and a roller disc from top to bottom.

[0007] Preferably, the transmission rod has a spiral drive groove extending downward on one end of the rod near the cutting motor, and a sliding sleeve is installed at the center of the movable disk, and is slidably connected to the spiral drive groove through the sliding sleeve; The transmission rod is mounted with a spline on its body located between the helical drive groove and the support frame, and the hob disc has a keyway running longitudinally through the center of the disc.

[0008] Preferably, a longitudinally arranged roller is rotatably connected to the inner wall of the sliding sleeve, and one radial end of the roller protrudes from the inner wall of the sliding sleeve and engages with and rolls with the helical drive groove.

[0009] Preferably, a plurality of silicone clips are installed at equal angles on the outer edge of the movable disc. Each silicone clip includes a fixed clip and a movable clip. A rotating shaft is provided between the fixed clip and the movable clip. A torsion spring is fitted on the rotating shaft. One end of the fixed clip is fixedly connected to the movable disc by a bolt. One end of the movable clip is located directly below the movable disc and is fitted with a silicone sleeve on its outer side. The other end passes around the rotating shaft and is connected to a lever. The two elastic arms of the torsion spring abut against the fixed clip and the lever, respectively.

[0010] Preferably, the cutter head is provided with a plurality of longitudinally penetrating cutter grooves at equal angles. A support rod is rotatably connected to the cutter grooves along the central axis of the radial direction of the cutter head. A cutting blade is fitted on the support rod, and the cutting edge of the cutting blade is located above the top surface of the cutter head.

[0011] Preferably, one end of the support rod passes through the outer peripheral sidewall of the cutter head and has an adjusting thread on the rod body. The support rod is connected to an angle adjusting nut through the adjusting thread.

[0012] Preferably, the filter assembly includes a fixed plate and a filter screen. The fixed plate is fixedly installed on the left and right side walls of the operation box, and a guide groove is provided inside the fixed plate along its length. A plurality of support sliders are slidably connected in the guide groove, and the ends of the support sliders opposite to the fixed plate are engaged with the edge of the filter screen.

[0013] Preferably, the support slider includes a guide wheel that is rolled in the guide groove. One end of the guide wheel passes through the fixed plate via a connecting column and is rotatably connected to a snap-fit ​​block. The snap-fit ​​block has a snap-fit ​​groove at the end opposite to the end connected to the guide wheel, and the snap-fit ​​groove engages with the edge of the filter screen.

[0014] Preferably, the front end of the control box is connected to a door via a hinge, and the door is provided with a transparent observation window. A sealing ring is also provided between the end faces of the control box and the door that are in contact with each other.

[0015] Compared with the prior art, this utility model provides a miniature ex vivo placental tissue cell extraction device, which has the following beneficial effects: This invention integrates a peeling and cutting mechanism, a filtering component, and a manifold inside the operating box, thereby integrating the functions of placental tissue peeling and cutting, filtering and separation, manifold collection, and external transportation into one unit. The operation of each component is coordinated and controlled by a control terminal, making the entire cell extraction process continuous and efficient, significantly shortening the cell extraction processing time of a single placental tissue, improving operational efficiency, and meeting the needs of large-scale target cell extraction.

[0016] This invention integrates a peeling and cutting mechanism, a filter assembly, and a manifold inside the operating box. The manifold extends the external collection box for storing cell suspension, minimizing the size of the operating box. This allows it to be placed in a biosafety cabinet for operation, effectively isolating external microorganisms and meeting the high requirements of placental hematopoietic cells for a sterile environment, thus ensuring the purity of the cell extraction process.

[0017] The control box, collection bucket, and internal metal components of this utility model are all made of high-pressure resistant 316L stainless steel, which is compatible with high-temperature sterilization and can ensure the cleanliness of the equipment through high-pressure steam sterilization. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a partial cross-sectional view of the control box of this utility model; Figure 3 This is a partial cross-sectional schematic diagram of the connection structure between the manifold and the collection bucket of this utility model; Figure 4 This is a three-dimensional structural diagram of the peeling and cutting mechanism of this utility model; Figure 5 This is a three-dimensional schematic diagram of the connection structure between the transmission rod and the support frame of this utility model. Figure 6 This is a front view schematic diagram of the drive connection structure between the transmission rod and the movable disc of this utility model; Figure 7 This is a three-dimensional structural diagram of the silicone clip of this utility model; Figure 8 This is a three-dimensional structural diagram of the hobbing disc of this utility model; Figure 9This is a three-dimensional structural diagram of the filter component of this utility model; Figure 10 For the present utility model Figure 4 Enlarged view of a portion of the structure at point A; Figure 11 For the present utility model Figure 6 Enlarged schematic diagram of the structure at point B in the middle; Figure 12 For the present utility model Figure 9 A magnified view of the structure at point C.

[0019] In the diagram: 1. Control terminal; 2. Operation box; 21. Box door; 211. Observation window; 3. Pressure pump; 4. Liquid level sensor; 5. Collection tank; 51. Acquisition valve; 6. Peeling and cutting mechanism; 61. Cutting motor; 62. Transmission rod; 621. Spiral drive groove; 622. Spline; 63. Movable disc; 631. Sliding sleeve; 632. Roller; 64. Silicone clamp; 641. Fixing clamp; 642. Rotating shaft; 643. Pulley; 644. Movable... 645. Movable clamp; 65. Silicone sleeve; 66. Roller disc; 67. Keyway; 68. Knife groove; 69. Support rod; 60. Adjusting thread; 61. Angle adjusting nut; 62. Cutter; 73. Support frame; 74. Filter assembly; 75. Fixing plate; 76. Guide groove; 77. Support slider; 78. Guide wheel; 79. Snap-fit ​​block; 70. Snap-fit ​​groove; 71. Filter screen; 82. Manifold; 83. Extension tube; 84. Connecting valve. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0021] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0022] Example, refer to Figure 1 - Figure 12A miniature ex vivo placental tissue cell extraction device includes an operation box 2 and a control terminal 1. Inside the operation box 2, from top to bottom, there are a peeling and cutting mechanism 6, a filter assembly 7, and a manifold 8. The peeling and cutting mechanism 6 is used to fix the ex vivo placental tissue and break it down. The filter assembly 7 is used to filter the tissue fragments and the tissue cell fluid inside, thereby achieving solid-liquid separation. The manifold 8 is used to initially collect the tissue fluid. The bottom of the manifold 8 is connected to an extension tube 81. The other end of the extension tube 81 passes through the side wall of the operation box 2 and is connected to a collection bucket 5 to expand the external capacity and thus collect the tissue fluid in a concentrated manner. A connecting valve 82, model DQW-03, is provided between the extension tube 81 and the operation box 2 to control the opening and closing of the connecting tube. A collection valve 51, model DQW-03, is provided on one side of the collection tank 5, with one end connected to the inside of the collection tank 5. The collection valve 51 is used to transfer the tissue fluid collected in the collection tank 5 to an external container for subsequent centrifugation. A pressure pump 3, model HSP11050, and a liquid level sensor 4, model L8000T001, are installed on the top surface of the collection tank 5. The pressure pump 3 can adjust the pressure inside the collection tank 5. By applying negative pressure to the collection tank 5, it absorbs tissue fluid from the manifold 8; by applying positive pressure to the collection tank 5, it pumps the tissue fluid out through the open collection valve 51 to an external centrifuge tube, facilitating subsequent centrifugation of the tissue fluid. The liquid level sensor 4 is used to monitor the liquid level of the tissue fluid inside the collection tank 5 to prevent it from overflowing. The control terminal 1 is located outside the operation box 2 and is electrically connected to the stripping and cutting mechanism 6, pressure pump 3, liquid level sensor 4, connecting valve 82, and collection valve 51. The control terminal 1 controls the operation of the stripping and cutting mechanism 6, pressure pump 3, and liquid level sensor 4 through electrical connection, so that after the placental tissue is stripped by the stripping and cutting mechanism 6, the stripped tissue is filtered through the filter assembly 7, so that the tissue fluid flows into the manifold 8, and then enters the extension tube 81 through the manifold 8 and finally flows into the collection bucket 5 for collection.

[0023] The operation box 2 has a high degree of internal component integration, which makes it small in size and can be placed in a biosafety cabinet for tissue and cell extraction. The metal parts in the operation box 2, collection bucket 5, as well as the internal peeling and cutting mechanism 6, filter assembly 7 and manifold 8 of the operation box 2 are made of high pressure resistant 316L stainless steel, which has good corrosion resistance and high temperature sterilization compatibility.

[0024] Furthermore, the stripping and cutting mechanism 6 includes a cutting motor 61, model YE3-132S-4, fixedly installed on the top wall inside the operating box 2. A transmission rod 62 is driven and connected to the bottom output end of the cutting motor 61. A support frame 67 is connected to the end of the transmission rod 62 via a bearing. Both ends of the support frame 67 are fixedly connected to the left and right side walls inside the operating box 2, respectively. A movable disc 63 and a rotary cutter disc 65 are sequentially driven and connected from top to bottom along the shaft of the transmission rod 62. In use, the cutting motor 61 acts as a power source to drive the transmission rod 62 to rotate. The end of the 2 is fixed to the inner wall of the operating box 2 by the support frame 67 to ensure that the two ends of its axis are consistent during rotation. The bottom end face of the movable disk 63 is used to fix and install the detached placental tissue. When the transmission rod 62 rotates, it synchronously drives the movable disk 63 on the rod to move up and down along the axis of the transmission rod 62, so that the movable disk 63 drives the detached placental tissue to move closer to or away from the roller cutter disk 65. At the same time, during the transmission process of the transmission rod 62, it synchronously drives the roller cutter disk 65 to rotate around its axis, so that the roller cutter disk 65 drives the cutter 66 to cut the detached placental tissue.

[0025] Furthermore, the transmission rod 62 has a spiral drive groove 621 extending downwards on one end of the rod near the cutting motor 61. A sliding sleeve 631 is installed at the center of the movable disk 63, and is slidably connected to the spiral drive groove 621 via the sliding sleeve 631. When the transmission rod 62 rotates, the spiral drive groove 621 engages with the sliding sleeve 631 of the movable disk 63, converting the rotational motion into axial movement of the movable disk 63 along the transmission rod 62. This allows the movable disk 63 to move the extracted placental tissue closer to the rotary cutter disk 65. As the movable disk 63 moves downwards... After moving to its limit position, it cooperates with the roller cutter disc 65 to clamp the detached placental tissue. Through rotation, the cutter 66 mounted on the roller cutter disc 65 cuts the detached placental tissue. During the cutting process, sufficiently small tissue fragments can fall into the filter assembly 7 through the gap between the cutter groove 652 on the roller cutter disc 65 and the cutter 66. Larger tissue fragments are still clamped between the movable disc 63 and the roller cutter disc 65 and are cut multiple times under the rotation of the roller cutter disc 65, thereby fully cutting and breaking down the larger tissue fragments.

[0026] The transmission rod 62 is located between the spiral drive groove 621 and the support frame 67 and is equipped with a spline 622. The center of the cutter head 65 is provided with a keyway 651 that runs longitudinally through the cutter head 65. One end of the transmission rod 62 passes through the keyway 651 in the center of the cutter head 65 and is rotatably connected to the support frame 67. The spline 622 and the keyway 651 are engaged and locked together to form a stable transmission structure, so that the cutter head 65 and the cutter 66 installed on the cutter head 65 can rotate synchronously with the transmission rod 62.

[0027] Furthermore, a longitudinally arranged roller 632 is rotatably connected to the inner wall of the sliding sleeve 631. One radial end of the roller 632 protrudes from the inner wall of the sliding sleeve 631 and engages with and rolls into the helical drive groove 621. The roller 632 on the inner wall of the sliding sleeve 631 engages with the helical drive groove 621 of the transmission rod 62, and the axial end face of the roller 632 is in contact with the end face of the helical drive groove 621. Thus, when the transmission rod 62 rotates, the roller 632 is driven to roll upward or downward along the helical path of the helical drive groove 621 by friction, thereby pushing the sliding sleeve 631 to drive the movable disk 63 to follow the movement, so that the movable disk 63 can move along the shaft of the transmission rod 62. Axial movement; when the roller 632 moves to the end of the path of the helical drive groove 621, the roller 632 can no longer push the movable disk 63 to move, and thus rotates between the sliding sleeve 631 and the helical drive groove 621, so that the movable disk 63 can still be kept in the limit position. When the transmission rod 62 drives the movable disk 63 to move downward, when the movable disk 63 moves to the bottom end of the helical drive groove 621 and cooperates with the hobbing disc 65 to clamp the detached placental tissue, the transmission rod 62 continues to drive the hobbing disc 65 to rotate, and there will be no transmission interference between it and the movable disk 63 that has reached the limit position, thus ensuring the normal operation of the cutting work.

[0028] Furthermore, several silicone clips 64 are installed at equal angles on the outer edge of the movable disc 63. Each silicone clip 64 includes a fixed clip 641 and a movable clip 644. A rotating shaft 642 is provided between the fixed clip 641 and the movable clip 644. A torsion spring is fitted onto the rotating shaft 642. One end of the fixed clip 641 is fixedly connected to the movable disc 63 by bolts. One end of the movable clip 644 is positioned directly below the movable disc 63 and is fitted with a silicone sleeve 645 on its outer side. The other end passes around the rotating shaft 642 and is connected to a lever 643. The two elastic arms of the torsion spring abut against the fixed clip 641 and the lever 643 respectively. The lever 643 and the movable clip 644 are connected in a V-shape. The connecting end between the lever 643 and the movable clip 644 is rotatably connected to the rotating shaft 642. The two elastic arms of the torsion spring abut against the fixed clip 641 and the lever 643 respectively. The opposing end faces of the fixed clamp 641 and the lever 643 abut against each other, thereby pushing the movable clamp 644 closer to the fixed clamp 641 under normal conditions through elastic force, so that the end face of the movable clamp 644 abuts against the bottom surface of the movable disc 63. The movable clamp 644 is fitted with a silicone sleeve 645 to avoid damage to the cells and tissues due to excessive local pressure during clamping. By pressing the lever 643, the movable clamp 644 rotates around the pivot 642, compressing the elastic arm of the torsion spring corresponding to one end of the lever 643, so that the movable clamp 644 can move away from the movable disc 63, thereby clamping and fixing the ex vivo placental tissue between the movable clamp 644 and the bottom surface of the movable disc 63. By setting multiple silicone clamps 64, the ex vivo placental tissue can be fully opened, which is convenient for the rotary cutter disc 65 to perform rotary cutting. In actual use, in order to avoid the collision between the cutter 66 installed on the disc body and the silicone clamp 64 during the cutting process of the hobbing disc 65, the silicone clamp 64 is fixedly installed on the outer edge of the movable disc 63.

[0029] Furthermore, the hobbing disc 65 is provided with several longitudinally penetrating grooves 652 at equal angles. A support rod 653 is rotatably connected to each groove 652 along the central axis of the radial direction of the hobbing disc 65. A cutter 66 is fitted onto the support rod 653. An interference fit is used between the support rod 653 and the cutter 66 to prevent the cutter 66 from freely rotating around the support rod 653 due to force after contact with the detached placental tissue, thus avoiding ineffective cutting. An interference fit is used to enable the rotating support rod 653 to drive the cutter 66 to move synchronously, allowing the angle between the cutter 66 and the end face of the roller cutter 65 to be adjusted, thereby adjusting the depth of cut per pass. The cutting edge of the cutter 66 is located above the top surface of the roller cutter 65. When the roller cutter 65 rotates with the transmission rod 62, the cutter 66 installed in the cutter groove 652 rotates synchronously. The cutting edge of the cutter 66 is higher than the top surface of the roller cutter 65. During the rotation, the cutting edge directly contacts and cuts the detached placental tissue.

[0030] Furthermore, one end of the support rod 653 penetrates the outer peripheral sidewall of the cutter head 65, and the rod body is provided with an adjusting thread 6531. The support rod 653 is engaged with an angle adjusting nut 654 through the adjusting thread 6531. When the cutter 66 is installed, it is located inside the cutter groove 652. One end of the support rod 653 passes through the outer peripheral sidewall of the cutter head 65 and enters the cutter groove 652, then passes through the cutter 66 and is rotatably connected to the sidewall of the cutter groove 652. The other end of the support rod 653 is located outside the outer peripheral sidewall of the cutter head 65, and the rod body is provided with an adjusting thread 6531. After the angle of the thread 6531 and the cutter 66 is adjusted to the correct position, in order to prevent the support rod 653 from rotating on its own, an angle adjusting nut 654 is screwed into the adjusting thread 6531. The angle adjusting nut 654 engages with the adjusting thread 6531, allowing the angle adjusting nut 654 to move axially along the direction of the support rod 653, thereby approaching and abutting against the outer peripheral side wall of the hobbing disc 65, thus locking the support rod 653 and preventing it from rotating on its own, thereby keeping the cutting depth of the cutter 66 constant during the cutting process.

[0031] Furthermore, the filter assembly 7 includes a fixing plate 71 and a filter screen 74. The filter screen 74 is used to filter the mixture of cut tissues. The fixing plate 71 is fixedly installed on the left and right side walls of the operation box 2. The fixing plate 71 has a guide groove 72 extending along its length inside. Several support sliders 73 are slidably connected in the guide groove 72. The end of the support sliders 73 that is away from the fixing plate 71 is engaged with the edge of the filter screen 74. The filter screen 74 is fixed by the support sliders 73. The support sliders 73 slide along the guide groove 72 of the fixing plate 71, thereby facilitating the installation, disassembly or replacement of the filter screen 74.

[0032] Furthermore, the support slider 73 includes a guide wheel 731 that is rolled in the guide groove 72. By setting the guide wheel 731 to roll in the guide groove 72, the resistance when installing or moving the filter screen 74 is reduced. One end of the guide wheel 731 passes through the fixed plate 71 via a connecting post and is rotatably connected to a snap-fit ​​block 732. The snap-fit ​​block 732 has a snap-fit ​​groove 733 at the end opposite to the end connected to the guide wheel 731. The snap-fit ​​groove 733 engages with the edge of the filter screen 74, so that the filter screen 74 and the support slider 73 can be quickly disassembled and installed.

[0033] Furthermore, the front end of the control box 2 is connected to the door 21 via a hinge, and the door 21 is provided with a transparent observation window 211. The observation window 211 allows real-time observation of the internal operating status. A sealing ring is also provided between the end faces of the control box 2 and the door 21 that are in contact with each other, so as to seal the contact area between the control box 2 and the door 21 when the control box 2 is closed, thereby isolating external contamination.

[0034] Working principle: Before use, the operation box 2 and its internal components are disassembled and sterilized by high-pressure steam. After sterilization, the components are reassembled in the biosafety cabinet and the assembly fit is checked to ensure that the tissue cell extraction operation is carried out in a sterile environment and to ensure sealing performance. The operation box 2 is connected to the control terminal 1 and the collection bucket 5 respectively by using wires and extension tubes 81 to pass through the biosafety cabinet.

[0035] During the process, open the door 21 of the control box 2, and press the lever 643 of the silicone clamp 64 on the outer edge of the movable disc 63 to rotate the movable clamp 644 around the rotating shaft 642 and move it away from the bottom surface of the movable disc 63. Place the detached placental tissue between the bottom surface of the movable disc 63 and the movable clamp 644. The placental tissue is flexibly clamped by the silicone sleeve 645 to ensure the stability of the tissue during the cutting process. After the detached placental tissue is fixed in place, close the door 21, and start the device through the external control terminal 1 to send a command to the cutting motor 61 to drive the transmission rod 62 to start rotating. When the transmission rod 62 rotates, the spiral drive groove 621 drives the movable disc 63 along the axis of the transmission rod 62 through the rollers 632 on the inner wall of the sliding sleeve 631. Moving downwards, the material gradually approaches the roller cutter disc 65. Simultaneously, the transmission rod 62 drives the roller cutter disc 65 to rotate synchronously through the engagement of the spline 622 and the keyway 651. The cutter 66 on the roller cutter disc 65 rotates at high speed with it. After the movable disc 63 moves to its limit position, it cooperates with the roller cutter disc 65 to clamp the placental tissue. The rotating cutter 66 cuts the tissue. Larger tissue fragments are clamped between the two and broken into small particles after multiple rotational cuts. Sufficiently small fragments fall through the gap between the cutter groove 652 of the roller cutter disc 65 and the cutter 66 to the filter screen 74 of the filter assembly 7. The filter screen 74 filters the mixture. Cell fluid passes through the filter screen 74 and is separated out, while the remaining tissue fragments are trapped by the filter screen 74.

[0036] The cell fluid filtered by filter 74 flows into the lower manifold 8, where it is initially collected. Control terminal 1 opens the connecting valve 82 on the extension tube 81 and simultaneously controls the pressure pump 3 of the collection tank 5 to start the negative pressure mode. The cell fluid in the manifold 8 enters the collection tank 5 through the extension tube 81. The liquid level sensor 4 on the top surface of the collection tank 5 monitors the liquid level of the cell fluid in real time and transmits the data to control terminal 1. If the liquid level gradually approaches the upper limit of the capacity of the collection tank 5, control terminal 1 will automatically prompt to prevent liquid overflow. When control terminal 1 shows that the amount of cell fluid in the collection tank 5 meets the requirements, it closes the connecting valve 82 to stop the liquid intake. When it is necessary to use external equipment, such as centrifuge tubes, to collect cell fluid, the centrifuge tubes or other external containers are connected to the collection valve 51. Control terminal 1 adjusts the pressure pump 3 to the positive pressure mode, closes the connecting valve 82, and opens the collection valve 51 on one side of the collection tank 5. The pressure pump 3 pressurizes the collection tank 5, so that the cell fluid in the collection tank 5 is pumped out through the collection valve 51 to the external centrifuge tube, completing the initial collection of cell fluid.

[0037] When cleaning the equipment, shut down all operating components through the control terminal 1, open the box door 21, remove the silicone clip 64 on the movable plate 63, clean the remaining tissue fragments, remove the filter screen 74 from the guide slide 72, clean the tissue residue on the filter screen 74, and then perform high-temperature sterilization treatment on the operating box 2 and its internal equipment again.

[0038] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A miniature ex vivo placental tissue cell extraction device, comprising an operation box (2) and a control terminal (1), characterized in that: The operating box (2) is equipped with a peeling and cutting mechanism (6), a filter assembly (7) and a manifold (8) from top to bottom. The bottom of the manifold (8) is connected to an extension tube (81). The other end of the extension tube (81) passes through the side wall of the operating box (2) and is connected to a collection bucket (5). A connecting valve (82) is provided between the extension tube (81) and the collection bucket (5). The collection tank (5) has a collection valve (51) on one side that is connected to the inside of the collection tank (5), and a pressure pump (3) and a liquid level sensor (4) are installed on the top surface of the collection tank (5). The control terminal (1) is located outside the operation box (2), and the control terminal (1) is electrically connected to the stripping and cutting mechanism (6), pressure pump (3), liquid level sensor (4), connecting valve (82), and acquisition valve (51).

2. The micro ex vivo placental tissue cell extraction device according to claim 1, wherein, The stripping and cutting mechanism (6) includes a cutting motor (61) fixedly installed on the top wall inside the operating box (2). The bottom output end of the cutting motor (61) is driven and connected to a transmission rod (62). The end of the transmission rod (62) is connected to a support frame (67) through a bearing. The two ends of the support frame (67) are fixedly connected to the left and right side walls inside the operating box (2), respectively. The rod body of the transmission rod (62) is driven and connected to a movable disc (63) and a roller disc (65) from top to bottom.

3. The micro ex vivo placental tissue cell extraction device of claim 2, wherein, The transmission rod (62) has a spiral drive groove (621) extending downwards on one end of the rod near the cutting motor (61). The movable disk (63) has a sliding sleeve (631) installed at the center of the disk and is slidably connected to the spiral drive groove (621) through the sliding sleeve (631). The transmission rod (62) is mounted with a spline (622) on its body between the helical drive groove (621) and the support frame (67), and the center of the hob disc (65) is provided with a keyway (651) that runs longitudinally through the hob disc (65).

4. The micro-extracorporeal placental tissue cell extraction device according to claim 3, wherein, A longitudinally arranged roller (632) is rotatably connected to the inner wall of the sliding sleeve (631). One radial end of the roller (632) protrudes from the inner wall of the sliding sleeve (631) and engages with and rolls with the spiral drive groove (621).

5. The micro-ex-vivo placental tissue cell extraction device according to claim 3, wherein, Several silicone clips (64) are installed at equal angles on the outer edge of the movable disc (63). Each silicone clip (64) includes a fixed clip (641) and a movable clip (644). A rotating shaft (642) is provided between the fixed clip (641) and the movable clip (644). A torsion spring is fitted on the rotating shaft (642). One end of the fixed clip (641) is fixedly connected to the movable disc (63) by bolts. One end of the movable clip (644) is located directly below the movable disc (63) and is fitted with a silicone sleeve (645) on its outer side. The other end passes around the rotating shaft (642) and is connected to a lever (643). The two elastic arms of the torsion spring abut against the fixed clip (641) and the lever (643) respectively.

6. The micro-ex-vivo placental tissue cell extraction device according to claim 3, wherein, The cutter head (65) is provided with several longitudinally penetrating cutter grooves (652) at equal angles. The cutter grooves (652) are rotatably connected to a support rod (653) along the central axis of the radial direction of the cutter head (65). A cutter (66) is fitted on the body of the support rod (653), and the cutting edge of the cutter (66) is located above the top surface of the cutter head (65).

7. The micro-ex-vivo placental tissue cell extraction device according to claim 6, wherein, One end of the support rod (653) passes through the outer peripheral side wall of the cutter head (65) and the rod body is provided with an adjusting thread (6531). The support rod (653) is connected to an angle adjusting nut (654) through the adjusting thread (6531).

8. The micro-ex-vivo placental tissue cell extraction device according to claim 1, wherein, The filter assembly (7) includes a fixed plate (71) and a filter screen (74). The fixed plate (71) is fixedly installed on the left and right side walls of the operation box (2). The fixed plate (71) has a guide groove (72) extending along its length inside. Several support sliders (73) are slidably connected in the guide groove (72). The support sliders (73) are engaged with the edge of the filter screen (74) at one end away from the fixed plate (71).

9. The micro ex vivo placental tissue cell extraction device of claim 8, wherein, The support slider (73) includes a guide wheel (731) that is rolled in the guide groove (72). One end of the guide wheel (731) passes through the fixed plate (71) via a connecting post and is rotatably connected to a snap-fit ​​block (732). The snap-fit ​​block (732) has a snap-fit ​​groove (733) at the end opposite to the end connected to the guide wheel (731). The snap-fit ​​groove (733) engages with the edge of the filter screen (74).

10. The micro-ex-vivo placental tissue cell extraction device according to claim 1, wherein, The front end of the operation box (2) is connected to a door (21) via a hinge, and the door (21) is provided with a transparent observation window (211). A sealing ring is also provided between the end faces of the operation box (2) and the door (21) that are in contact with each other.

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