A multi-chamber cell bag

Abstract

This invention discloses a multi-chamber cell bag, relating to a cell culture bag that is placed flat on a platform for culturing cell spheroids. The bag includes a main body containing multiple compartments for culturing cell spheroids and a main chamber for collecting culture and reducing cell spheroid density. The compartments and the main chamber are connected by a channel with controllable on / off states. Each compartment has a first sample inlet and a first drain outlet, while the main chamber has a second sample inlet, a sampling outlet, and a second drain outlet. Both the compartments and the main chamber have first and second ventilation ports, respectively. This invention induces high-density cell spheroids in the compartments before collecting them in the main chamber for low-density culture, catering to the environmental needs of different growth stages of cell spheroids. This improves cell spheroid formation rate and culture quality, providing high-quality experimental materials for cell spheroid-related research.

Description

Technical Field

[0001] This utility model relates to a cell bag, specifically a multi-chamber cell bag. Background Technology

[0002] In cell sphere culture technology, cells and culture medium are usually injected into the same cell bag for culture, and then the cell spheres are removed from the cell bag after they have grown into spheres.

[0003] Cell spheroids require high-density culture in the initial stage. Cells interact to form spheroid structures, and each mesenchymal stem cell gradually stabilizes the spheroid structure through the secretion of extracellular matrix proteins. However, due to the initial high-density culture, the number of spheroid units per unit volume is high, leading to easy fusion between spheroids after formation, severely affecting the uniformity of spheroid size. Scale-up the culture system after spheroid unit formation can reduce the spheroid content per unit volume, decrease direct fusion of spheroids, and thus maintain size uniformity. Currently, no existing cell bags can achieve these functions. Utility Model Content

[0004] The purpose of this invention is to provide a multi-chamber cell bag to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A multi-chamber cell bag, placed flat on a platform, is used for culturing cell spheres. It includes a cell bag body containing multiple sub-chambers for culturing cell spheres and a main chamber for collecting culture and reducing cell sphere density. The sub-chambers and the main chamber are connected by a channel with controllable on / off states. Each sub-chamber has a first inlet for adding cells and culture medium and a first outlet for discharging culture waste liquid. The main chamber has a second inlet for adding culture medium, a sampling port for removing cell spheres, and a second outlet for discharging culture waste liquid. The sub-chambers and the main chamber each have a first and a second ventilator for gas exchange during cell sphere culture.

[0007] Compared with the prior art, the beneficial effects of this utility model are:

[0008] This invention first induces high-density cell spheroids in separate chambers, and then collects them into a main chamber for low-density culture. This meets the environmental requirements of different growth stages of cell spheroids, which helps to improve the cell spheroid formation rate and culture quality, and provides high-quality experimental materials for cell spheroid-related research. Attached Figure Description

[0009] Figure 1This is a top view of a multi-chambered cell bag.

[0010] Figure 2 This is a bottom view of a multi-chambered cell bag. Detailed Implementation

[0011] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0012] In this embodiment of the invention, a multi-chamber cell bag, placed flat on a platform, is used for culturing cell spheres, particularly for culturing mesenchymal stem cell spheres. The cell bag body 1 includes a cell bag body 1 containing multiple sub-cavities 2 for culturing cell spheres and a main cavity 3 for collecting culture and reducing cell sphere density. The sub-cavities 2 and the main cavity 3 are connected by a channel 4 with controllable on / off states. Each sub-cavity 2 has a first inlet 5 for adding cells and culture medium and a first outlet 9 for discharging culture waste liquid from the sub-cavity 2. The main cavity 3 has a second inlet 7 for adding culture medium, a sampling port 10 for removing cell spheres, and a second outlet 11 for discharging culture waste liquid from the main cavity 3. The sub-cavities 2 and the main cavity 3 each have a first gas exchange port 6 and a second gas exchange port 8 for exchanging gases during cell sphere culture.

[0013] like Figure 1 and Figure 2 As shown, the lower part of the cell bag body 1 is a large total cavity 3, and the upper part of the cell bag body 1 is a plurality of parallel sub-cavities 2. In addition to this form, the sub-cavities 2 can be distributed around the total cavity 3, or they can be distributed in a three-dimensional form on the outside of the total cavity 3. Other forms are not listed here.

[0014] The total cavity 3 has a capacity greater than the sum of the capacities of all the sub-cavities 2, preferably 1.5-3 times the sum of the capacities of the sub-cavities 2; the cell spheres have a higher density in the sub-cavities 2, and the density of the cell spheres can be reduced after the cell spheres that have undergone preliminary culture enter the total cavity 3.

[0015] Both the first drain port 9 and the second drain port 11 are equipped with filters (not marked in the figure) to prevent cell balls from being discharged during waste liquid discharge. When discharging waste liquid, the cell balls are trapped in the sub-cavity 2 / total cavity 3.

[0016] The mesh diameter of the filter is no greater than 0.5μm. Since the size of a single mesenchymal stem cell is usually greater than 10μm, which is larger than the mesh diameter, when draining the fluid (such as when replacing the waste fluid in compartment 2 or the main compartment 3), the waste fluid can flow out through the filter, while the mesenchymal stem cells and cell spheres are retained, effectively preventing the cells from being lost with the waste fluid.

[0017] A valve (not shown in the figure) is installed in the channel 4 between the main cavity 3 and the sub-cavity 2 to control the opening and closing of the channel. The channel 4 is a rigid pipe. When the cells are cultured into spheres in the sub-cavity 2, the valve is closed. After the initial culture is completed, the culture bag is erected (sub-cavity 2 on top and main cavity 3 on the bottom) to allow the cell spheres and culture medium to gather around the valve. The valve is then opened, and the cell spheres and culture medium in the gas-filled sub-cavity 2 are forced into the main cavity 3 by the gas pressure in the sub-cavity 2. After the cell spheres and culture medium in the sub-cavity 2 have entered the main cavity 3, the valve is closed.

[0018] In this embodiment, the connection points of the first sample inlet 5, the first air exchange port 6, the second sample inlet 7, and the second air exchange port 8 with the cell bag body 1 are located on the upper surface of the cell bag body 1; the connection points of the first drain port 9, the sampling port 10, and the second drain port 11 with the cell bag body 1 are located on the lower surface of the cell bag body 1.

[0019] The first sample inlet 5, the first air exchange port 6, the second sample inlet 7, the second air exchange port 8, the first drain port 9, the sampling port 10, and the second drain port 11 are all equipped with sealing elements. Preferably, the sealing elements on the first sample inlet 5, the second sample inlet 7, the first drain port 9, the sampling port 10, and the second drain port 11 are sealing nuts; while the sealing elements on the first air exchange port 6 and the second air exchange port 8 are shut-off reversing valves.

[0020] It should be noted that the cell bag body in this application is made of common medical-grade polyester and other transparent materials. When culturing cell spheres, cells and culture medium are first injected into each sub-cavity 2 for preliminary high-cell-density culture. After the cell spheres undergo preliminary culture, they enter the total cavity 3, which can reduce the density of cell spheres. During the culture process, whether it is sub-cavity 2 or total cavity 3, culture waste liquid needs to be discharged from the first drain port 9 / second drain port 11 at regular intervals, and new culture medium needs to be injected from the first sample inlet 5 / second sample inlet 7. After the culture is completed, the cell spheres in the total cavity 3 are taken out from the sampling port 10.

[0021] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention. Furthermore, the contents not described in detail in this specification are all prior art known to those skilled in the art.

Claims

1. A multi-chamber cell bag, placed flat on a platform, for culturing cell spheres, characterized in that, The system includes a cell bag body (1), which contains multiple sub-cavities (2) for culturing cell spheres and a total cavity (3) for collecting culture and reducing cell sphere density. The sub-cavities (2) and the total cavity (3) are connected by a channel (4) with controllable on / off state. Each sub-cavity (2) has a first inlet (5) for adding cells and culture medium into the sub-cavity (2) and a first drain outlet (9) for discharging culture waste liquid from the sub-cavity (2). The total cavity (3) has a second inlet (7) for adding culture medium into the total cavity (3), a sampling port (10) for removing cell spheres, and a second drain outlet (11) for discharging culture waste liquid from the total cavity (3). The sub-cavities (2) and the total cavity (3) have a first gas exchange port (6) and a second gas exchange port (8) for exchanging gases during cell sphere culture, respectively.

2. The multi-chamber cell bag according to claim 1, characterized in that, The total capacity of the main cavity (3) is greater than the sum of the capacities of all the sub-cavities (2).

3. The multi-chamber cell bag according to claim 1, characterized in that, Both the first drain port (9) and the second drain port (11) are fitted with filters to prevent cell spheres from being discharged during waste liquid discharge.

4. A multi-chamber cell bag according to claim 3, characterized in that, The mesh diameter of the filter screen is no greater than 0.5 μm.

5. A multi-chamber cell bag according to claim 1, characterized in that, The passage (4) between the main cavity (3) and the sub-cavities (2) is equipped with a valve that can control the opening and closing of the passage.

6. A multi-chamber cell bag according to claim 1, characterized in that, The connection points of the first sample inlet (5), the first air exchange port (6), the second sample inlet (7), and the second air exchange port (8) with the cell bag body (1) are located on the upper surface of the cell bag body (1); the connection points of the first drain port (9), the sampling port (10), and the second drain port (11) with the cell bag body (1) are located on the lower surface of the cell bag body (1).

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