A cell culture bioreactor

Abstract

This utility model relates to the field of cell culture technology, specifically to a cell culture bioreactor; it includes a base, a culture cylinder, a top cover, a discharge valve, and a light-shielding mechanism. The light-shielding mechanism includes a mounting plate, a linear guide rail, a linear slider, an upper protective cover, a lower protective cover, a connecting frame, a locking pin, an exhaust pipe, a stirring device, and a feeding device. During cell culture, cultured cells and cell culture fluid are added to the culture cylinder through the feeding device. The mounting plate, linear guide rail, linear slider, and locking pin structure allow for adjustment of the fit between the upper and lower protective covers and the culture cylinder, thereby enabling light-shielding and non-light-shielding treatment of the culture cylinder. The stirring device facilitates stirring, and the discharge valve is used for discharging materials. This invention addresses the problem that existing bioreactors are mostly made of transparent materials, which facilitate observation of the reaction by staff, but the cell culture medium contains light-sensitive components that affect cell culture under light conditions.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a bioreactor for cell culture. Background Technology

[0002] Currently, bioreactors are equipped with multi-layer packed beds. The nutrient solution can flow from top to bottom or overflow from bottom to top, which can lead to uneven distribution of the nutrient solution. The packed bed at the tail end cannot absorb enough nutrients, which affects the cell culture effect.

[0003] A search revealed that prior art CN215799620U discloses a bioreactor for cell culture, comprising a shell, a transmission mechanism, a cell culture packing bed, and an infusion tube. The shell has inlets and outlets, the transmission mechanism is located inside the shell, and the infusion tube passes through the upper wall of the shell. The cell culture packing bed includes a packing bed carrier box and a packing bed body. The top and front sides of the packing bed carrier box have openings, and a baffle is rotatably connected to the front side of the packing bed carrier box. The packing bed body is located inside the packing bed carrier box. In this cell culture bioreactor, when the packing bed carrier box moves to below the second infusion tube, the motor is turned off, and nutrient solution flows into the packing bed body through the first infusion tube connected to the outside. Since multiple packing bed bodies within the device can directly receive the nutrient solution, the problem of uneven nutrient solution distribution caused by different heights of the packing beds in the prior art is solved, which is beneficial for cell culture.

[0004] However, the above-mentioned equipment has the following problems in actual use: during cell culture, light and the mixing of cells with nutrient solution affect cell growth. Most existing bioreactors are made of transparent materials, which makes it convenient for staff to observe the reaction. However, cell culture media contain light-sensitive components, which affect cell culture under light conditions. Utility Model Content

[0005] The purpose of this invention is to provide a cell culture bioreactor that addresses the problem that existing bioreactors are mostly made of transparent materials, which facilitates observation of the reaction by staff, but the cell culture medium contains light-sensitive components that affect cell culture under light conditions.

[0006] To achieve the above objectives, this utility model provides a cell culture bioreactor, including a base and a culture tube. The top of the culture tube is equipped with a detachable top cover, and the bottom is provided with a discharge valve. The top cover is opaque and also includes a light-shielding mechanism.

[0007] The light-shielding mechanism includes a mounting plate, a linear guide rail, a linear slider, an upper cover, a lower cover, a connecting frame, a locking pin, an exhaust pipe, a stirring device, and a feeding device. The mounting plate is installed on both sides of the base, the linear guide rail is installed on the mounting plate, and the linear slider can slide linearly on the linear guide rail. The upper cover is located on the top side of the culture cylinder and is slidably connected to the base, and is symmetrically arranged. The lower cover is integrally formed with the upper cover and is located on the bottom side of the culture cylinder, and is slidably connected to the base. The connecting frame is detachably connected to the U-shaped connecting arms of the upper cover and the lower cover and the linear slider, respectively. The locking pin is slidably connected to the mating block on one side of the linear slider, and is threaded through and connected to the base. The exhaust pipe is screwed onto the top of the top cover. The stirring device is located on one side of the culture cylinder, and the feeding device is located on the side of the top cover near the stirring device.

[0008] The stirring device includes a stirring motor and a stirring frame. The stirring motor is mounted on the top cover, and the stirring frame is mounted on the output shaft of the stirring motor and located inside the culture cylinder.

[0009] The feeding device includes a feeding pipe and a threaded cap. The feeding pipe is screwed onto the top of the top cover, and the threaded cap is screwed onto the top of the feeding pipe.

[0010] The exhaust pipe has a filter screen installed at the top air outlet.

[0011] The cell culture bioreactor further includes a limiting mechanism, which includes a first limiting post and a second limiting post. The first limiting post is threadedly connected to the base and abuts against one side of the linear guide rail; the second limiting post is threadedly connected to the base and abuts against the end of the linear guide rail away from the first limiting post.

[0012] This invention relates to a cell culture bioreactor. During cell culture, cultured cells and cell culture medium are added to the culture cylinder via a feeding device. The upper and lower protective covers can be adjusted to fit the culture cylinder using a mounting plate, linear guide rail, linear slider, and locking pin structure, allowing for both light-blocking and light-free operation. A stirring device facilitates stirring, and a discharge valve allows for material discharge. This invention addresses the problem that existing bioreactors, often made of transparent materials, are convenient for observation, but the cell culture medium contains light-sensitive components that negatively impact cell culture under light conditions. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0014] Figure 1 This is a schematic diagram of the overall structure of the cell culture bioreactor according to the first embodiment of this utility model.

[0015] Figure 2 This is the first embodiment of the present utility model. Figure 1 Enlarged view of point A in the middle.

[0016] Figure 3 This is a schematic diagram of the structure of the stirring rack according to the first embodiment of this utility model.

[0017] Figure 4 This is a schematic diagram of the overall structure of the cell culture bioreactor according to the second embodiment of this utility model.

[0018] Figure 5 This is the second embodiment of the present utility model. Figure 4 Enlarged view of point B in the middle.

[0019] In the diagram: 101-base, 102-culture cylinder, 103-top cover, 104-discharge valve, 105-mounting plate, 106-linear guide rail, 107-linear slider, 108-upper cover, 109-lower cover, 110-connecting frame, 111-locking pin, 112-exhaust pipe, 113-stirring motor, 114-stirring frame, 115-feeding pipe, 116-threaded cap, 201-first limiting post, 202-second limiting post. Detailed Implementation

[0020] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0021] Example 1:

[0022] like Figures 1 to 3 As shown, where Figure 1 This is a schematic diagram of the overall structure of a cell culture bioreactor. Figure 2 yes Figure 1 Enlarged view of point A in the middle. Figure 3This is a schematic diagram of the structure of the stirring rack 114. This utility model provides a cell culture bioreactor: including a base 101, a culture cylinder 102, a top cover 103, a discharge valve 104, and a light-shielding mechanism. The light-shielding mechanism includes a mounting plate 105, a linear guide rail 106, a linear slider 107, an upper protective cover 108, a lower protective cover 109, a connecting frame 110, a locking pin 111, an exhaust pipe 112, a stirring device, and a feeding device. The stirring device includes a stirring motor 113 and a stirring rack 114, and the feeding device includes a feeding pipe 115 and a threaded cap 116. This solution addresses the problem that existing bioreactors are mostly made of transparent materials, facilitating observation of the reaction, but cell culture media contain light-sensitive components that affect cell culture under light. Therefore, this solution allows for light-shielding of the culture cylinder 102.

[0023] In this embodiment, the culture tube 102 has a detachable top cover 103 installed on its top and a discharge valve 104 at its bottom, both of which are transparent. The top cover 103 is opaque. The end of the mounting ring on the culture tube 102 is fixed with countersunk bolts. The discharge valve 104 is used for discharging material, and the top cover 103 is used for sealing and can be fixed with bolts. The base 101 has symmetrically arranged mounting holes for easy fixing with bolts.

[0024] The mounting plate 105 is installed on both sides of the base 101, the linear guide rail 106 is installed on the mounting plate 105, the linear slider 107 can slide linearly on the linear guide rail 106, the upper cover 108 is disposed on the top side of the culture cylinder 102 and is slidably connected to the base 101, and is symmetrically arranged, the lower cover 109 is integrally formed with the upper cover 108 and is disposed on the bottom side of the culture cylinder 102 and is slidably connected to the base 101, the connecting bracket 110 is detachably connected to the U-shaped connecting support arm of the upper cover 108 and the lower cover 109 and the linear slider 107, the locking pin 111 is slidably connected to the mating block on one side of the linear slider 107 and is disposed through it, and is threadedly connected to the base 101, the exhaust pipe 112 is screwed onto the top of the top cover 103, the stirring device is disposed on one side of the culture cylinder 102, and the feeding device is disposed on the side of the top cover 103 near the stirring device. The mounting plate 105 is fixed with bolts, and the linear guide rail 106 is fixed with countersunk bolts. A linear slider 107 capable of linear sliding is provided on the linear guide rail 106. The linear slider 107 only slides linearly and does not move vertically. An L-shaped mating block is detachably installed on one side of the linear slider 107. The mating block has two vertical through holes, and a threaded hole is provided on the base 101 below the through holes. Both the upper cover 108 and the lower cover 109 are assembled structures, forming an integral cover structure by the two covers abutting against each other. The connecting frame 110 has a U-shaped structure. The top is connected and fixed to the U-shaped connecting arms of the upper cover 108 and the lower cover 109 by bolts. The bottom is connected to the top mounting part of the linear slider 107 by bolts. The locking pin 111 has a T-shaped structure. The bottom external thread end directly engages with the threaded hole on the base 101 to limit the linear slider 107. The bottom threaded end of the exhaust pipe 112 is directly installed in the threaded connection hole of the top cover 103. The stirring device is used for stirring, and the feeding device is used for feeding.

[0025] Secondly, the stirring motor 113 is mounted on the top cover 103; the stirring frame 114 is mounted on the output shaft of the stirring motor 113 and located inside the culture cylinder 102. The stirring motor 113 is fixed by bolts, and the stirring frame 114 is connected and fixed to its output shaft by a coupling. The bottom of the stirring frame 114 is provided with multiple stirring rods.

[0026] Then, the feeding pipe 115 is screwed onto the top of the top cover 103; the threaded cap 116 is screwed onto the top of the feeding pipe 115. The top of the feeding pipe 115 is provided with an external thread end to facilitate the installation of the threaded cap 116. The threaded cap 116 is used for sealing after feeding to reduce the entry of external impurities into the interior. An oxygen supply pipe is provided on the top cover 103. The oxygen supply pipe inlet is located close to the inner side wall of the culture cylinder 102 to avoid interference with the stirring rod when the stirring rack 114 rotates. The bottom of the oxygen supply pipe is a bend to facilitate the fit with the tapered structure at the bottom of the culture cylinder 102. The air outlet is located close to the inner bottom of the culture cylinder 102, which also does not interfere with the rotation of the stirring rack 114. The top inlet of the oxygen supply pipe is connected to a gas manufacturing device, such as an oxygen generator, to supply oxygen.

[0027] Finally, a filter screen is provided on the top outlet of the exhaust pipe 112. This structure facilitates exhaust while reducing the entry of external impurities into the interior.

[0028] This invention addresses the problem that existing bioreactors, mostly made of transparent materials for easy observation of the reaction, suffer from the issue of light-sensitive components in cell culture media affecting cell culture. During cell culture, the threaded cap 116 is removed, and cultured cells and cell solution are added to the culture cylinder 102 via the feeding pipe 115. At this time, the discharge valve 104 is closed, and the lower and upper protective covers 109 and 108 are not providing protection. Then, when light protection is needed, the connecting frame 110 is pulled, causing the linear slider 107 to move, thereby moving the upper and lower protective covers 108 and 109. The components approach each other and finally come into contact to form a protective cavity, which provides light-blocking treatment for the transparent culture cylinder 102. When the upper protective cover 108 and the lower protective cover 109 abut against their respective halves, the linear slider 107 can be limited by the locking pin 111, thereby enabling the culture cylinder 102 to be either light-blocked or not. The stirring device facilitates low-speed stirring, and the discharge valve 104 can be used for material discharge. This solves the problem that existing bioreactors are mostly made of transparent materials, which are convenient for staff to observe the reaction, but the cell culture medium contains light-sensitive components that affect cell culture under light conditions.

[0029] Example 2:

[0030] like Figure 4 and Figure 5 As shown, where Figure 4 This is a schematic diagram of the overall structure of a cell culture bioreactor. Figure 5 yes Figure 4The enlarged view at point B shows that, based on the first embodiment, this utility model provides a cell culture bioreactor, which further includes a limiting mechanism, comprising a first limiting post 201 and a second limiting post 202.

[0031] The first limiting post 201 is threadedly connected to the base 101 and abuts against one side of the linear guide rail 106; the second limiting post 202 is threadedly connected to the base 101 and abuts against the end of the linear guide rail 106 away from the first limiting post 201. The first limiting post 201 and the second limiting post 202 are the same size, and their bottom external threaded ends can be directly installed on the base 101. Their outer curved surfaces abut against the two side planes of the linear guide rail 106, respectively. The top end faces of the first limiting post 201 and the second limiting post 202 are provided with internal hexagonal holes for easy installation or removal by rotating with an internal hexagonal wrench.

[0032] In this embodiment, by installing the first limiting post 201 and the second limiting post 202, the linear slider 107 can be limited to prevent it from falling off when it slides to both ends of the linear guide rail 106.

[0033] The above-disclosed embodiments are merely one or more preferred embodiments of this application and should not be construed as limiting the scope of this application. Those skilled in the art can understand that all or part of the processes for implementing the above embodiments and equivalent changes made in accordance with the claims of this application still fall within the scope of this application.

Claims

1. A cell culture bioreactor, comprising a base and a culture tube, wherein a removable top cover is mounted on the top of the culture tube, and a discharge valve is provided at the bottom, and the top cover is opaque, characterized in that: It also includes a light-shielding mechanism; The light-shielding mechanism includes a mounting plate, a linear guide rail, a linear slider, an upper cover, a lower cover, a connecting frame, a locking pin, an exhaust pipe, a stirring device, and a feeding device. The mounting plate is installed on both sides of the base, the linear guide rail is installed on the mounting plate, and the linear slider can slide linearly on the linear guide rail. The upper cover is located on the top side of the culture cylinder and is slidably connected to the base, and is symmetrically arranged. The lower cover is integrally formed with the upper cover and is located on the bottom side of the culture cylinder, and is slidably connected to the base. The connecting frame is detachably connected to the U-shaped connecting arms of the upper cover and the lower cover and the linear slider, respectively. The locking pin is slidably connected to the mating block on one side of the linear slider, and is threaded through and connected to the base. The exhaust pipe is screwed onto the top of the top cover. The stirring device is located on one side of the culture cylinder, and the feeding device is located on the side of the top cover near the stirring device.

2. The cell culture bioreactor as described in claim 1, characterized in that: The stirring device includes a stirring motor and a stirring frame. The stirring motor is mounted on the top cover; the stirring frame is mounted on the output shaft of the stirring motor and is located inside the culture tube.

3. The cell culture bioreactor as described in claim 1, characterized in that: The feeding device includes a feeding pipe and a threaded cap. The feeding pipe is screwed onto the top of the top cover; the threaded cap is screwed onto the top of the feeding pipe.

4. The cell culture bioreactor as described in claim 1, characterized in that: A filter screen is installed on the top air outlet of the exhaust pipe.

5. The bioreactor for cell culture of claim 1, wherein : The cell culture bioreactor further includes a limiting mechanism, which includes a first limiting post and a second limiting post. The first limiting post is threadedly connected to the base and abuts against one side of the linear guide rail; the second limiting post is threadedly connected to the base and abuts against the end of the linear guide rail away from the first limiting post.

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