A tissue embedding cassette

Abstract

This utility model relates to the field of embedding cassettes, and more particularly to a tissue embedding cassette. It includes: a main body box, a lid, and a multi-cavity tissue fixation structure. One end of the lid is rotatably connected to one end of the main body box. The multi-cavity tissue fixation structure is sandwiched within the cavity formed by the lid and the main body box. The multi-cavity tissue fixation structure includes multiple spaced-apart independent tissue placement cavities. Each tissue placement cavity is an annular cylindrical structure composed of a thin-walled material, with open ports at both ends. The opening direction of the tissue placement cavities is consistent with the opening direction of the ventilation holes on the grid plate in the main body box. In this utility model, because the internal space of the tissue placement cavities is smaller, it can further restrict the large-scale movement of tissue sections during centrifugation and dehydration, thereby reducing the risk of deformation and damage to the tissue sections. Simultaneously, because each tissue placement cavity is independently spaced, it can prevent tissue sections from piling up during centrifugation and dehydration.

Description

Technical Field

[0001] This utility model relates to the field of embedding cassettes, and more particularly to a tissue embedding cassette. Background Technology

[0002] Medical tissue embedding cassettes are essential tools in pathology laboratories for processing and preserving biological tissue samples. They primarily support a series of pretreatment steps before sectioning, including dehydration, clearing, and paraffin embedding, ensuring that samples can be accurately sliced ​​into thin sections for subsequent microscopic examination. Tissue samples processed in this way help physicians make accurate diagnoses and develop appropriate treatment plans. Medical tissue embedding cassettes are widely used in pathology laboratories of hospitals, research institutions, and third-party testing centers, playing a vital role in disease diagnosis and research.

[0003] In clinical dermatology practice, hair loss is one of the most common skin diseases. The "gold standard" for diagnosing various hair loss conditions is to perform a scalp biopsy of the affected area to determine the type, extent, and histological characteristics of the disease. Unlike traditional skin biopsies, which typically consist of larger blocks of tissue such as the epidermis, dermis, and subcutaneous tissue, scalp biopsies are often prepared as two 4mm cylinders, each with a specific sectioning direction and histological observation, including longitudinal sections (for assessing the integrity of different hair follicle structures and the hair cycle status) and transverse sections (for assessing the number, density, and characteristic changes of hair follicles at different horizontal cross-sections). Therefore, the preparation and embedding process of scalp biopsies differs significantly from that of traditional biopsies in terms of technical details, placing higher demands on accurate sample placement and standardized tissue orientation.

[0004] Specifically, in clinical practice, to obtain longitudinal and transverse sections that meet diagnostic requirements, tissues must be placed and fixed in an embedding cassette with a specific orientation, followed by dehydration, clearing, and paraffin embedding. For example... Figure 1 As shown, traditional biopsy specimen embedding cassettes are mostly single-sided openings with a rectangular cavity inside, without partitions. They are only suitable for the routine processing of larger tissue blocks and cannot meet the processing steps required for small scalp tissues during embedding. They also cannot meet the need for precise control of tissue orientation within the embedding cassette, which significantly affects the quality of subsequent tissue sections and diagnostic accuracy.

[0005] In addition, the cavity inside a traditional tissue embedding cassette is a rectangular space. When multiple tissue slices are placed inside for centrifugation and dehydration, the tissue slices will move randomly, which can easily cause the slices to deform, be damaged, or pile up. Summary of the Invention

[0006] In view of this, the present invention provides a tissue embedding cassette, which at least partially solves the problems existing in the prior art.

[0007] According to one aspect of the present invention, a tissue embedding cassette is provided, comprising:

[0008] Main body box, box cover and multi-cavity tissue fixation structure.

[0009] One end of the lid is rotatably connected to one end of the main body box. A multi-cavity tissue fixing structure is sandwiched in the cavity formed by the lid and the main body box.

[0010] The multi-cavity tissue fixation structure includes multiple spaced-apart independent tissue placement cavities. Each tissue placement cavity is a thin-walled annular cylindrical structure with open ports at both ends. The opening direction of the tissue placement cavity is consistent with the opening direction of the ventilation holes on the grid plate in the main body box.

[0011] Furthermore, the bottom of the tissue placement cavity is embedded and fixed to the grid plate.

[0012] Furthermore, the top height of the tissue placement cavity is less than the top height of the cavity formed by the lid and the main body box.

[0013] The multi-cavity tissue fixation structure also includes: multiple reinforcing ribs.

[0014] Multiple reinforcing ribs are fixedly installed on the inner side of the lid, and are used to press against the top of multiple tissue placement cavities when the lid and the main body are closed.

[0015] Furthermore, the multi-cavity tissue fixation structure also includes a fixation frame.

[0016] The fixation frame is a frame structure, and multiple tissue placement cavities are embedded and fixed within the fixation frame.

[0017] The fixture and the tissue placement cavity form an independent structure, sandwiched between the lid and the main body box.

[0018] Furthermore, multiple support frames are installed near the bottom of the tissue placement cavity, and the support frames have a hollow structure.

[0019] Furthermore, a recessed structure is provided on one end of the fixing frame, which is used to engage with the protrusion in the main body box.

[0020] Furthermore, multiple clamping strips are provided on the inner sidewall of the tissue placement cavity along the depth direction, with both ends of the clamping strips extending to the upper and lower ends of the tissue placement cavity, respectively.

[0021] Furthermore, the clamping strip has a chamfer at one end near the top of the tissue placement cavity.

[0022] Furthermore, the clamping strips are connected to the frame structure in the raised frame.

[0023] Furthermore, the tissue placement cavity is a circular annular cylindrical structure or a rectangular annular cylindrical structure.

[0024] The technical solution of this utility model has at least the following beneficial effects:

[0025] This utility model proposes an improved embedding device design specifically for scalp tissue embedding scenarios. Taking into account the small size and precise orientation requirements of clinical scalp biopsy tissues, as well as the need for pre-treatment through liquid infiltration, dehydration, and transparency before fixation, this patented design proposes a tissue placement cavity structure with openings at both the top and bottom. This structure allows liquid to fully and evenly enter from the openings at the top and bottom of the embedding cassette, ensuring thorough contact and infiltration of the hair tissue specimen.

[0026] Furthermore, by setting multiple independent tissue placement chambers at intervals within the cavity formed by the lid and the main body, tissue slices can be placed in different chambers during use. Because the internal space of each tissue placement chamber is smaller, it further restricts significant movement of the tissue slices during centrifugation and dehydration, thereby reducing the risk of deformation and damage. This ensures accurate positioning and orientation of the samples during subsequent slicing, preventing tissue deformation or displacement. Simultaneously, because each tissue placement chamber is independently spaced, the tissue slices placed within them remain at relatively independent intervals, thus preventing them from piling up during centrifugation and dehydration.

[0027] Furthermore, it is proposed that the opening direction of the tissue placement cavity be consistent with the opening direction of the ventilation holes on the grid plate of the embedding cassette, thereby effectively enhancing the flow efficiency of the liquid processing medium, improving the tissue infiltration effect during the embedding process, and ensuring that hair tissue specimens obtain accurate and stable section quality during longitudinal and transverse sections, providing more reliable histological evidence for clinical diagnosis. Attached Figure Description

[0028] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0029] Figure 1 This is a schematic diagram of the structure of an existing tissue embedding cassette with its lid open in the background art.

[0030] Figure 2 This is a schematic diagram of the structure of the tissue embedding cassette in one of the corresponding embodiments of this application.

[0031] Figure 3This is a schematic diagram of the back structure of the main body box in one of the corresponding embodiments of this application.

[0032] Figure 4 This is a schematic diagram of the structure of the tissue embedding cassette in the third embodiment of this application.

[0033] Figure 5 This is a front view of the independent structure formed by the fixation frame and the tissue placement cavity in one embodiment of this application.

[0034] Figure 6 This is a schematic diagram of the rear structure of the independent structure formed by the fixation frame and the tissue placement cavity in one embodiment of this application.

[0035] Figure Labels

[0036] 1. Box lid; 2. Main box; 21. Grid plate; 22. Ventilation hole; 23. Protrusion; 31. Tissue placement cavity; 32. Reinforcing rib; 33. Fixing frame; 34. Elevation frame; 35. Clamping strip; 36. Concave structure; 4. Tissue section. Detailed Implementation

[0037] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0038] It should be noted that, unless otherwise specified, the following embodiments and features can be combined with each other. Furthermore, all other embodiments obtained by those skilled in the art based on the embodiments of this disclosure without inventive effort are within the scope of protection of this disclosure.

[0039] It should be noted that various aspects of the embodiments described below are within the scope of the appended claims. It will be apparent that the aspects described herein can be embodied in a wide variety of forms, and any particular structure and / or function described herein is merely illustrative. Based on this disclosure, those skilled in the art will understand that one aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number of aspects set forth herein can be used to implement the device and / or practice the method. Additionally, this device and / or method can be implemented using other structures and / or functionalities besides one or more of the aspects set forth herein.

[0040] As one embodiment of this utility model, such as Figures 2 to 6 As shown, a tissue embedding cassette is provided, including: a main body cassette 2, a cassette cover 1, and a multi-cavity tissue fixation structure.

[0041] One end of the lid 1 is rotatably connected to one end of the main body box 2. The multi-cavity tissue fixing structure is clamped in the cavity formed by the lid 1 and the main body box 2.

[0042] like Figure 2 and Figure 4 As shown, the multi-cavity tissue fixation structure includes multiple spaced-apart independent tissue placement cavities 31. Each tissue placement cavity 31 is an annular cylindrical structure composed of a thin-walled core, with open ports at both ends. The opening direction of the tissue placement cavity 31 is consistent with the opening direction of the ventilation holes 22 on the grid plate 21 in the main body box 2.

[0043] By providing multiple independent tissue placement chambers 31 at intervals within the cavity formed by the lid 1 and the main body 2, tissue slices 4 can be placed in different tissue placement chambers 31 during use. Specifically, the tissue placement chambers 31 are either circular or rectangular annular cylindrical structures to accommodate tissue slices 4 of different shapes. Because the internal space of the tissue placement chambers 31 is smaller, it further restricts the significant movement of the tissue slices 4 during centrifugation and dehydration, thereby reducing the risk of deformation and damage to the tissue slices 4. Furthermore, since each tissue placement chamber 31 is independently spaced, the tissue slices 4 placed within them will remain in relatively independent intervals, thus preventing the tissue slices 4 from piling up during centrifugation and dehydration.

[0044] Specifically, the construction and connection methods of multi-cavity tissue fixation structures can take the following forms:

[0045] Firstly

[0046] like Figure 2 and Figure 3 As shown, in this embodiment, the bottom of the tissue placement cavity 31 is embedded and fixed to the grid plate 21. This allows for the fixed installation of multiple independent placement cavities within the cavity formed by the lid 1 and the main body 2. Typically, to further prevent the tissue slices 4 from detaching from the top of the tissue placement cavity 31, the top height of the tissue placement cavity 31 can be set to the same height as the top of the cavity formed by the lid 1 and the main body 2. Thus, the top of the tissue placement cavity 31 can be sealed after the lid 1 is closed.

[0047] Secondly

[0048] As the depth of the tissue placement cavity 31 increases, the internal space of the cavity also increases, which in turn increases the space for the tissue slice 4 to move. However, during use, the tissue slice 4 is still prone to deformation or damage due to collisions during movement. Therefore, based on this, it is necessary to reduce the depth of the tissue placement cavity 31, that is, the top height of the tissue placement cavity 31 should be less than the top height of the cavity formed by the cover 1 and the main body 2.

[0049] like Figure 2 As shown, the multi-cavity tissue fixation structure also includes multiple reinforcing ribs 32.

[0050] Multiple reinforcing ribs 32 are fixedly disposed on the inner side of the lid 1. Normally, when the lid 1 is closed, the multiple reinforcing ribs 32 are located directly above the tissue placement cavity 31, and are used to press against the top of the multiple tissue placement cavities 31 when the lid 1 and the main body box 2 are closed.

[0051] In this embodiment, by providing reinforcing ribs 32 on the inner side of the lid 1, the top of the tissue placement cavity 31 is sealed, and the strength of the lid 1 is also improved. In addition, due to the constraint of the reinforcing ribs 32, the shrinkage deformation problem after the lid 1 is injection molded can also be reduced, thereby improving the manufacturing qualification rate of the product.

[0052] The two structures described in "one" and "two" of this application are more suitable for manufacturing together with the main body box 2 and the box cover 1. That is, they are more suitable for development and manufacturing as a new tissue embedding box structure.

[0053] Third

[0054] like Figures 4 to 6 As shown, the multi-cavity tissue fixation structure also includes a fixation frame 33.

[0055] The fixing frame 33 is a frame structure, and multiple tissue placement cavities 31 are embedded and fixed within the fixing frame 33. The fixing frame 33 and the tissue placement cavities 31 form an independent structure, which is sandwiched between the box cover 1 and the main box 2.

[0056] Because, in this embodiment, in order to clamp the fixture 33 and the tissue placement cavity 31 between the lid 1 and the main body 2, the tissue placement cavity 31 has a greater depth. Therefore, in order to reduce the movement space of the tissue slice 4, further, as... Figure 5 and Figure 6 As shown, multiple support frames 34 are provided on the inner side of the tissue placement cavity 31 near the bottom, and the support frames 34 have a hollow structure.

[0057] At the same time, such as Figure 5 As shown, in order to ensure that the fixing frame 33 does not move significantly in the cavity between the box cover 1 and the main body box 2, a concave structure 36 is provided on one side end of the fixing frame 33. The concave structure 36 is used to be engaged with the protrusion 23 in the main body box 2.

[0058] Furthermore, such as Figure 5 As shown, multiple clamping strips 35 are provided along the depth direction on the inner wall of the tissue placement cavity 31, with both ends of the clamping strips 35 extending to the upper and lower ends of the tissue placement cavity 31, respectively. The clamping strips 35 are connected to the frame structure in the support frame 34.

[0059] By setting multiple clamping strips 35, the tissue slice 4 can be further confined. Simultaneously, more space can be created between the tissue slice 4 and the inner wall of the tissue placement cavity 31, facilitating the smooth drainage of water during centrifugation. Preferably, the clamping strip 35 has a chamfered end near the top of the tissue placement cavity 31. This chamfer makes it easier to place the tissue slice 4 into the tissue placement cavity 31.

[0060] In the embodiment corresponding to "third" of this application, the fixture 33 and the tissue placement cavity 31 form an independent structure, which can be adapted to be clamped in various existing embedding cassettes, and thus can be used to improve existing embedding cassettes.

[0061] The above are merely specific embodiments of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the scope of the claims.

Claims

1. A tissue embedding cassette, characterized in that, include: Main box, box lid, and multi-cavity tissue fixation structure; One side of the lid is rotatably connected to one side of the main body box; The multi-cavity tissue fixation structure is sandwiched in the cavity formed by the lid and the main body box; The multi-cavity tissue fixation structure includes multiple independent tissue placement cavities spaced apart. Each tissue placement cavity is an annular cylindrical structure made of a thin-walled material, with open ports at both ends. The opening direction of the tissue placement cavity is consistent with the opening direction of the ventilation holes on the grid plate in the main body box.

2. The tissue embedding cassette according to claim 1, characterized in that, The bottom of the tissue placement cavity is embedded and fixed to the grid plate.

3. A tissue embedding cassette according to claim 2, characterized in that, The top height of the tissue placement cavity is less than the top height of the cavity formed by the lid and the main body box; The multi-cavity tissue fixation structure also includes: multiple reinforcing ribs; Multiple reinforcing ribs are fixedly disposed on the inner side of the box lid, and are used to press against the top of multiple tissue placement cavities when the box lid and the main box are closed.

4. A tissue embedding cassette according to claim 1, characterized in that, The multi-cavity tissue fixation structure also includes a fixation frame; The fixation frame is a frame structure, and multiple tissue placement cavities are embedded and fixed within the fixation frame; The fixture and the tissue placement cavity form an independent structure, which is sandwiched between the box cover and the main box.

5. A tissue embedding cassette according to claim 4, characterized in that, Multiple support frames are provided near the bottom of the tissue placement cavity, and the support frames have a hollow structure.

6. A tissue embedding cassette according to claim 4, characterized in that, One end of the fixing frame is provided with a concave structure, which is used to engage with the protrusion in the main body box.

7. A tissue embedding cassette according to claim 5, characterized in that, Multiple clamping strips are provided on the inner sidewall of the tissue placement cavity along the depth direction, and the two ends of the clamping strips extend to the upper and lower ends of the tissue placement cavity, respectively.

8. A tissue embedding cassette according to claim 7, characterized in that, The clamping strip has a chamfer at one end near the top of the tissue placement cavity.

9. A tissue embedding cassette according to claim 7, characterized in that, The clamping strip is connected to the frame structure in the raised frame.

10. A tissue embedding cassette according to claim 1, characterized in that, The tissue placement cavity is a circular or rectangular annular cylindrical structure.

Images