A device for zebrafish diving behavior research

Abstract

The application discloses a device for studying diving behavior of zebrafish, and relates to the technical field of fish research devices.The device comprises a research assembly, wherein the research assembly comprises a water tank, an electric sliding table, a push plate and a sieve plate.The water tank is a cuboid with an open upper end, the sieve plate is fixed in the water tank, and the sieve plate is spaced apart from the bottom of the water tank.The electric sliding table is fixed in the water tank and located above the sieve plate.The push plate is fixed on the sliding block of the electric sliding table, and the electric sliding table is used to drive the push plate to move and reduce the swimming range of fish.The lower side of the push plate is tightly attached to the sieve plate.The lower side of the push plate is tightly attached to the sieve plate, and the mesh of the sieve plate is smaller than the volume of the fish, so that the fish is prevented from being clamped into the gap, and water flow is allowed to pass through, thereby avoiding water pressure accumulation.The grid of the observation plate and the double-scale cm grid facilitate quantification of the position of the fish group.The height scale cm records the diving depth, and the volume scale mL monitors the water volume change, so as to provide accurate data for behavior research.

Description

Technical Field

[0001] This invention relates to the field of fish research equipment technology, and in particular to an apparatus for studying the diving behavior of zebrafish. Background Technology

[0002] Zebrafish are small freshwater fish with 87% genetic similarity to humans, making them suitable for modeling various human diseases. Zebrafish are easy to raise, inexpensive, easy to observe pharmacologically, and highly reproducible, making them an important model organism with promising applications in evaluating the efficacy of drugs for anti-anxiety, sleep promotion, and learning / cognitive abilities. However, the dispersed movement of zebrafish along the depth of the tank (Z-axis) and the difficulty in tracking their trajectories lead to significant errors in accurately recording their movements, affecting data collection and analysis by behavioral recorders.

[0003] Traditionally, research on zebrafish diving behavior has relied on simple tank setups, which often only provide a basic feeding and observation environment and lack the ability to precisely control experimental conditions and systematically collect behavioral data.

[0004] In experiments, to more accurately observe and analyze zebrafish behavior, it is often necessary to confine them to a specific spatial area. However, traditional tank designs lack effective fish control mechanisms, and researchers typically rely on manual capture or simple physical barriers to restrict fish activity. This is not only inefficient but may also harm the fish, affecting the accuracy of experimental results.

[0005] When using physical means such as push plates to restrict the movement of fish, if there is a distance between the side and bottom of the push plate and the water tank, the fish will get stuck between the push plate and the water tank; if there is no distance between the side and bottom of the push plate and the water tank, a large amount of water will accumulate on one side during the push plate's movement, causing the water to overflow the water tank and the fish to escape. Summary of the Invention

[0006] This application provides a device for studying the diving behavior of zebrafish. It solves the problems in existing technologies where, when using physical means such as push plates to restrict fish movement, if there is a distance between the sides and bottom of the push plate and the water tank, fish may get stuck. Conversely, if there is no distance, a large amount of water accumulates on one side during the push, causing water to overflow and fish to escape. The device achieves a design where the lower side of the push plate is in close contact with a sieve plate, and the mesh size of the sieve plate is smaller than the fish's volume. This prevents fish from getting stuck in gaps while allowing water flow and avoiding water pressure buildup. The grid and dual-scale cm grid on the observation plate facilitate quantification of fish location. The height scale (cm) records diving depth, and the volume scale (mL) monitors water volume changes, providing accurate data for behavioral studies. The camera stand is compatible with multiple devices, supporting mobile phones, infrared cameras, and natural light cameras to meet recording needs under different lighting conditions.

[0007] This application provides an apparatus for studying the diving behavior of zebrafish, including research components; the research components include a water tank, an electric slide, a push plate, and a sieve plate; The water tank is a rectangular prism with an opening at the top. The sieve plate is fixed inside the water tank, and there is a gap between the sieve plate and the bottom of the water tank. The electric sliding table is fixed inside the water tank and is located above the sieve plate; The push plate is fixed on the slider of the electric slide table, which is used to move the push plate to reduce the swimming range of the fish. The lower side of the push plate is in close contact with the screen plate.

[0008] As an improvement, the water tank stores aquaculture water, and the electric slide is located above the water surface. The movement direction of the slider inside the electric slide is parallel to the ground. The length-to-width ratio of the water tank is 4:1; The mesh size of the sieve plate is smaller than the volume of the fish.

[0009] As an improvement, the research components also include an observation port, an observation plate, a cover plate, and vents; An observation port is opened on one side of the water tank, and an observation plate is fixed inside the observation port. The surface of the observation plate has a grid, which is square with a side length of 5 cm. The surface of the observation plate is engraved with height and volume scales. The cover plate is rotatably connected to the water tank. The cover plate has multiple air holes that are evenly spaced. The water tank, observation panel, and cover are all made of glass.

[0010] As improvements, a filter assembly and a camera stand are also included; The filter assembly is fixed inside the water tank on the side adjacent to the observation port; The filtration assembly includes a heating rod, a circulation pump, a filter, and delivery tubing; The heating element, circulation pump, and filter are all fixed to one side of the water tank, with the inlet of the circulation pump extending below the sieve plate. The delivery pipe is connected to the output end of the circulating pump, and the end of the delivery pipe furthest from the circulating pump is connected to the input end of the filter. The filter and its output are both located above the water level in the tank; The camera stand is set up on one side of the observation board. The camera stand is used to hold a mobile phone, an infrared camera or a natural light camera to record and observe the movement of the fish. The mobile phone, infrared camera, and natural light camera recorded videos in MP4, AVI, and MOV formats, respectively.

[0011] As an improvement, the research components also include protective component one and protective component two; Protective component one includes airbag one and air pump one; protective component two includes airbag two, air pump two and waterproof box. The first airbag is C-shaped and is fixed inside the water tank and to the side of the observation panel. The C-shaped opening of airbag one faces the push plate; The side of the push plate is in close contact with the airbag. The air pump is fixed outside the water tank, and the output end of the air pump is connected to the air bag. The second airbag is fixed on the side of the push plate away from the filter assembly, and the waterproof box is fixed on the side of the push plate close to the filter assembly. The top of the waterproof box is open, and the top of the waterproof box is located above the horizontal plane inside the water tank. The second air pump is fixed inside the waterproof box, and the output end of the second air pump is connected to the second airbag.

[0012] As an improvement, both airbag one and airbag two are made of liquid silicone rubber; Airbag 1 and Airbag 2 are above the water level inside the tank; In the initial state, airbag one and airbag two are in a fully contracted state.

[0013] As an improvement, protective component one includes a separator membrane one, and protective component two includes a separator membrane two; The first separator is C-shaped and is fixed inside the first airbag. There are multiple first separators, which are evenly spaced from top to bottom to divide the first airbag into multiple individual expansion cavities. The number of air pumps is consistent with the number of individual expansion cavities in the airbags separated by the separator membrane, and they correspond one-to-one. The output end of the air pump is connected to the individual expansion cavities in the airbags separated by the separator membrane. The second separator membrane is fixed inside the second airbag. There are multiple second separator membranes, which are evenly spaced from top to bottom to divide the second airbag into multiple individual expansion cavities. The number of air pumps two is the same as the number of individual expansion cavities in airbags two separated by the separator membrane two, and they correspond one-to-one. The output end of air pump two is connected to the individual expansion cavities in airbags two separated by the separator membrane two. Both separator membrane 1 and separator membrane 2 are made of liquid silicone rubber.

[0014] One or more technical solutions provided in the embodiments of this application have at least the following technical effects or advantages: Firstly, the lower side of the push plate is tightly attached to the sieve plate, and the mesh size of the sieve plate is smaller than the volume of the fish, which prevents the fish from getting stuck in the gaps while allowing water to flow through and avoiding water pressure buildup. The grid and dual-gradient cm grid of the observation plate facilitate the quantification of fish school positions. The height scale in cm records diving depth, and the volume scale in mL monitors water volume changes, providing accurate data for behavioral studies. The camera stand is compatible with multiple devices, supporting mobile phones, infrared cameras, and natural light cameras to meet the recording needs under different lighting conditions. Secondly, the C-shaped airbag is fixed to the inner wall of the water tank and the side of the observation plate, with its opening facing the push plate. It cushions the impact of the fish when the push plate moves. The second airbag is installed on the side of the push plate to further reduce the direct collision between the fish and the push plate. It is made of liquid silicone rubber, which has high transparency and does not affect observation. Its softness protects the fish from damage. Thirdly, airbag one contains multiple C-shaped dividing membranes, and airbag two contains a dividing membrane, dividing airbag one and airbag two into independent expansion cavities. Each expansion cavity corresponds to an independent air pump one and air pump two, achieving precise layered control. The bottom-up driving strategy inflates the bottom expansion cavity, pushing the bottom fish to the surface and blocking their escape path. The top expansion cavity inflates, concentrating the fish at the surface for easy capture. The sequential inflation and deflation of each layer of expansion cavity creates a progressive driving force, significantly improving fishing efficiency. The independent inflation of each expansion cavity avoids the problem of weak bottom protection caused by airbag one and airbag two floating together, ensuring uniform protection throughout the entire water depth. Attached Figure Description

[0015] Figure 1 This is a perspective view of a device for studying the diving behavior of zebrafish according to the present invention; Figure 2 This is a three-dimensional sectional view of the water tank of a device for studying the diving behavior of zebrafish according to the present invention; Figure 3 This is a schematic diagram of the installation of a filter assembly for studying the diving behavior of zebrafish according to the present invention. Figure 1 ; Figure 4 This is a schematic diagram of the installation of a filter assembly for studying the diving behavior of zebrafish according to the present invention. Figure 2 ; Figure 5 This is a schematic diagram of the installation of airbag 1 and airbag 2 of the device for studying the diving behavior of zebrafish according to the present invention. Figure 6 This is a top view of the airbag installation of a device for studying the diving behavior of zebrafish according to the present invention.

[0016] In the diagram: 100, Research component; 110, Water tank; 111, Observation port; 120, Observation plate; 130, Cover plate; 131, Air vent; 140, Electric sliding table; 150, Push plate; 160, Protective component one; 161, Airbag one; 162, Separator membrane one; 163, Air pump one; 170, Protective component two; 171, Airbag two; 172, Separator membrane two; 173, Air pump two; 174, Waterproof box; 180, Sieve plate; 200, Filter assembly; 210, Heating rod; 220, Circulation pump; 230, Filter; 240, Delivery pipe; 300, Camera stand. Detailed Implementation

[0017] To facilitate understanding of the present invention, a more complete description of this application will be given below with reference to the accompanying drawings, which illustrate preferred embodiments of the invention. However, the invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to enable a more thorough and complete understanding of the disclosure of the present invention.

[0018] It should be noted that the terms "vertical," "horizontal," "up," "down," "left," "right," and similar expressions used in this article are for illustrative purposes only and do not represent the only possible implementation.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains; the terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to limit the invention; the term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0020] Example 1: As Figures 1-4 As shown, this application discloses an apparatus for studying the diving behavior of zebrafish, including a research component 100, a filter component 200, and a camera stand 300; the research component 100 includes a water tank 110, an observation port 111, an observation plate 120, a cover plate 130, an air hole 131, an electric slide 140, a push plate 150, and a sieve plate 180. The water tank 110 is a cuboid with an opening at the top. The sieve plate 180 is fixed inside the water tank 110, and there is a distance between the sieve plate 180 and the bottom of the water tank 110. The length-to-width ratio of water tank 110 is 4:1; The mesh size of the 180 mesh plate is smaller than the volume of the fish.

[0021] Specifically, the mesh size of the sieve plate 180 is smaller than the volume of the fish, which can collect the feces produced by the zebrafish while preventing the fish from getting stuck in the mesh size of the sieve plate 180. The electric slide table 140 is fixed inside the water tank 110 and is located above the sieve plate 180. The push plate 150 is fixed on the slider of the electric slide table 140. The electric slide table 140 is used to drive the push plate 150 to move and reduce the swimming range of the fish. The lower side of the push plate 150 is in close contact with the screen plate 180.

[0022] The water tank 110 stores aquaculture water, and the electric slide 140 is located above the water surface. The movement direction of the slider in the electric slide 140 is parallel to the ground. Specifically, when it is necessary to move the zebrafish in the tank 110, the electric slide 140 is started, which drives the push plate 150 fixed on the electric slide 140 to move, continuously reducing the activity range of the zebrafish, thereby quickly collecting and gathering them.

[0023] An observation port 111 is provided on one side of the water tank 110. An observation plate 120 is fixed inside the observation port 111. The surface of the observation plate 120 has a grid. The grid is square and the side length is 5 cm. The surface of the observation plate 120 is engraved with height and volume scales. The cover plate 130 is rotatably connected to the water tank 110. The cover plate 130 has multiple air holes 131, which are evenly spaced. The water tank 110, observation plate 120 and cover plate 130 are all made of glass.

[0024] Specifically, the grid lines facilitate observation of the zebrafish moving to specific scale positions in the tank 110, making recording easier; the height scale on the observation plate 120 is in cm, and the volume scale is in mL, allowing for observation of the zebrafish's movement trajectory distance and water content within the tank 110 by observing the height and volume scales respectively; the transparent glass material facilitates the observation of the zebrafish's movement trajectory, and the vent 131 ensures that the air inside the tank 110 is connected to the outside air, preventing oxygen deficiency.

[0025] The filter assembly 200 is fixed inside the water tank 110 on the side adjacent to the observation port 111; The filter assembly 200 includes a heating rod 210, a circulation pump 220, a filter 230, and a delivery pipe 240; The heating rod 210, the circulation pump 220 and the filter 230 are all fixed on one side inside the water tank 110, and the input end of the circulation pump 220 extends into the bottom of the sieve plate 180. The delivery pipe 240 is connected to the output end of the circulation pump 220, and the end of the delivery pipe 240 away from the circulation pump 220 is connected to the input end of the filter 230; Filter 230 and its output are both located above the water level in water tank 110; The heating power of the heating rod 210 is 50W to 200W, and the filter 230 is equipped with a filter pool and an ultraviolet sterilization module. Specifically, the heating rod 210 controls the heating power to facilitate the adjustment of the water temperature in the water tank 110, which is suitable for experimental use and also prevents zebrafish from dying due to excessively high or low water temperatures. The water in the water tank 110 is continuously drawn into the filter 230 by the circulation pump 220 and then flows back into the water tank 110, achieving the purpose of continuous purification and oxygenation of the water in the water tank 110, thus ensuring the aquatic environment for the zebrafish to live in.

[0026] The camera stand 300 is set on one side of the observation board 120. The camera stand 300 is used to place a mobile phone, an infrared camera or a natural light camera to record and observe the movement trajectory of the fish.

[0027] Specifically, the camera stand 300 is located on one side of the long side of the tank 110, and the narrow and long tank 110 makes it easy to observe and monitor the movement range of the zebrafish.

[0028] The mobile phone, infrared camera, and natural light camera recorded videos in MP4, AVI, and MOV formats, respectively.

[0029] Specifically, a mobile phone, infrared camera, or natural light camera placed on the camera stand 300 records the behavior of zebrafish during their diving process.

[0030] Videos recorded on mobile phones are in MP4 format and can be directly imported into Tracker software for behavioral trajectory analysis, thereby evaluating the zebrafish's anxiety, fear, and other behaviors.

[0031] The video recorded by the infrared camera is in AVI format and can be directly imported into ImageJ / TrackMate software for behavioral trajectory analysis, thereby evaluating the zebrafish's anxiety, fear, and other behaviors.

[0032] Videos recorded by natural light cameras in MOV format can be directly imported into ZebraZoom software for behavioral trajectory analysis, thereby evaluating zebrafish's anxiety, fear, and other behaviors.

[0033] The heating rod 210, the circulation pump 220 and the filter 230 are all existing technologies and will not be described in detail here.

[0034] The technical solutions described in the embodiments of this application have at least the following technical effects or advantages: The push plate 150 is flush against the sieve plate 180, and the mesh size of the sieve plate 180 is smaller than the volume of the fish. This prevents fish from getting stuck in the gaps while allowing water to flow through, avoiding water pressure buildup. The grid and dual-gradient 5cm grid of the observation plate 120 facilitate the quantification of fish school positions. The height scale (cm) records diving depth, and the volume scale (mL) monitors water volume changes, providing accurate data for behavioral studies. The camera stand 300 is compatible with multiple devices, supporting mobile phones, infrared cameras, and natural light cameras to meet recording needs under different lighting conditions.

[0035] Example 2: In the above embodiment, the movement of the push plate 150 continuously reduces the zebrafish's activity space, making it easier to catch them. However, during use, as the space shrinks, the zebrafish become frightened and scatter, colliding with the water tank 110, observation plate 120, and push plate 150, resulting in injury and affecting research. Therefore, the solution in Example 1 is improved, such as... Figures 5-6 As shown: Research component 100 also includes protective component one 160 and protective component two 170; Protective component 160 includes airbag 161 and air pump 163, and protective component 2 170 includes airbag 2 171, air pump 2 173 and waterproof box 174. The airbag 161 is C-shaped and is fixed inside the water tank 110 and on the side of the observation plate 120. The C-shaped opening of the airbag 161 faces the push plate 150; The push plate 150 is in close contact with the side of the airbag 161; Air pump 163 is fixed outside water tank 110, and the output end of air pump 163 is connected to air bag 161. Airbag 171 is fixed on the side of push plate 150 away from filter assembly 200, and waterproof box 174 is fixed on the side of push plate 150 close to filter assembly 200. The upper end of waterproof box 174 is open and the upper end of waterproof box 174 is located above the horizontal plane inside water tank 110. Air pump 2 173 is fixed inside waterproof box 174, and the output end of air pump 2 173 is connected to airbag 2 171.

[0036] Specifically, the waterproof box 174 has an opening at the top, and the top of the waterproof box 174 is located above the horizontal plane inside the water tank 110. This prevents water from entering and damaging the air pump 173 while supplying air to the air pump 173.

[0037] Both airbag 161 and airbag 2171 are made of liquid silicone rubber; Airbag 161 and airbag 2171 are above the water level inside water tank 110; In the initial state, airbag 161 and airbag 2171 are in a fully contracted state.

[0038] Specifically, when the pusher plate 150 moves, reducing the zebrafish's activity space and requiring it to be moved, air pumps 163 and 173 inflate air bladders 161 and 171. Furthermore, as the pusher plate 150 moves continuously, the gas inside air bladder 161 decreases, preventing the pusher plate 150 from pressing tightly against air bladder 161, which would cause the compressed air bladder 161 to have a smaller and smaller expansion space and a larger and larger expansion degree. The continuous movement of the pusher plate 150 ensures that the gas inside air bladder 161 remains at the same level of expansion. The inner walls of airbag 161 and airbag 2171 are made of a material that can prevent damage when the zebrafish hits them; and the liquid silicone rubber made of airbag 161 and airbag 2171 has high transparency and light transmittance, so it will not affect the observation of the zebrafish's activity inside.

[0039] The technical solutions described in the embodiments of this application have at least the following technical effects or advantages: C-shaped airbag 161 is fixed to the inner wall of the water tank 110 and the side of the observation plate 120, with the opening facing the push plate 150. It buffers the impact of the fish when the push plate 150 moves. Airbag 2 171 is installed on the side of the push plate 150 to further reduce the direct collision between the fish and the push plate 150. It is made of liquid silicone rubber, which has high transparency and does not affect the observation. Its softness protects the fish from damage.

[0040] Example 3: In the above examples, airbag 161 and airbag 2171 are used to protect zebrafish. However, during use, even after the pusher plate 150 adjusts the zebrafish's activity space, catching the zebrafish inside remains difficult. Furthermore, after inflating, airbags 161 and 171 have significant buoyancy, resulting in greater expansion at the upper end and less at the lower end, leading to poorer protection for the zebrafish at the lower end. Therefore, the solution in Example 2 is improved, such as... Figure 5 As shown: Protective component 160 includes separator membrane 162, and protective component 2 170 includes separator membrane 2 172; The separator membrane 162 is C-shaped and is fixed inside the airbag 161. There are multiple separator membranes 162, which are evenly spaced from top to bottom to divide the airbag 161 into multiple individual expansion cavities. The number of air pumps 163 is the same as the number of individual expansion cavities in airbags 161 separated by the separator membrane 162, and they correspond one-to-one. The output end of air pump 163 is connected to the individual expansion cavities in airbags 161 separated by the separator membrane 162. Separating membrane 2 172 is fixed inside airbag 2 171. There are multiple separating membranes 2 172, which are evenly spaced from top to bottom to divide airbag 2 171 into multiple individual expansion cavities. The number of air pumps 2173 is consistent with the number of individual expansion cavities in airbags 2171 separated by the separator membrane 2172, and they correspond one-to-one. The output end of air pumps 2173 is connected to the individual expansion cavities in airbags 2171 separated by the separator membrane 2172. Specifically, after the pusher plate 150 moves, the expansion cavity separated by the dividing membrane 162 at the bottom of airbag 161 continuously expands and contracts, and the expansion cavity separated by the dividing membrane 172 at the bottom of airbag 171 continuously expands and contracts, driving the zebrafish at the bottom to the top; and when the fish at the bottom are completely driven to the top, the expansion cavities at the bottom of airbag 161 and airbag 171 expand, causing airbag 161 and airbag 171 to collide with each other, blocking the zebrafish's return path. From bottom to top, multiple expansion cavities work continuously, driving the zebrafish to the top and concentrating the fish group at the water surface, making it convenient for catching operations; Furthermore, the overall buoyancy of the individual expansion cavities separated by airbag 161 and airbag 2171 is reduced, avoiding the problem of a larger expansion degree at the upper end and a smaller expansion degree at the lower end, resulting in poor protection for zebrafish at the lower end.

[0041] Separator membrane 162 and separator membrane 272 are both made of liquid silicone rubber.

[0042] Specifically, the liquid silicone rubber separators 162 and 172 have high transparency and light transmittance, and will not affect the observation of the zebrafish's activity inside.

[0043] The technical solutions described in the embodiments of this application have at least the following technical effects or advantages: Airbag 161 contains multiple C-shaped separator membranes 162, and airbag 2 171 contains a separator membrane 172, dividing airbags 161 and 171 into independent expansion cavities. Each expansion cavity corresponds to an independent air pump 163 or air pump 173, achieving precise layered control. The bottom-up driving strategy inflates the bottom expansion cavities, pushing bottom-dwelling fish to the surface and blocking their escape routes. The top expansion cavities inflate, concentrating the fish at the surface for easier capture. The sequential inflation and deflation of each layer of expansion cavities creates a progressive driving force, significantly improving harvesting efficiency. Independent inflation of each expansion cavity avoids the problem of weak bottom protection caused by the overall floating of airbags 161 and 171, ensuring uniform protection throughout the entire water depth.

[0044] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. For those skilled in the art, the present invention can have various modifications and variations. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A device for studying the diving behavior of zebrafish, characterized in that, The research component (100) includes a water tank (110), an electric slide (140), a push plate (150), and a sieve plate (180). The water tank (110) is a cuboid with an opening at the top. The sieve plate (180) is fixed inside the water tank (110), and there is a distance between the sieve plate (180) and the bottom of the water tank (110). The electric slide (140) is fixed inside the water tank (110) and is located above the sieve plate (180); The push plate (150) is fixed on the slider of the electric slide table (140). The electric slide table (140) is used to drive the push plate (150) to move and reduce the swimming range of the fish. The lower side of the push plate (150) is in close contact with the sieve plate (180).

2. The apparatus for studying the diving behavior of zebrafish as described in claim 1, characterized in that, The water tank (110) stores aquaculture water, and the electric slide (140) is located above the water surface. The direction of movement of the slider in the electric slide (140) is parallel to the ground. The length-to-width ratio of the water tank (110) is 4:1; The mesh size of the sieve plate (180) is smaller than the volume of the fish.

3. The apparatus for studying the diving behavior of zebrafish as described in claim 1, characterized in that, The research component (100) also includes an observation port (111), an observation plate (120), a cover plate (130), and an air vent (131). An observation port (111) is opened on one side of the water tank (110), and an observation plate (120) is fixed inside the observation port (111). The surface of the observation plate (120) has a grid, which is square and has a side length of 5 cm. The surface of the observation plate (120) is engraved with height and volume scales. The cover plate (130) is rotatably connected to the water tank (110). The cover plate (130) has multiple air holes (131) that are evenly spaced. The water tank (110), observation plate (120) and cover plate (130) are all made of glass.

4. The apparatus for studying the diving behavior of zebrafish as described in claim 1, characterized in that, It also includes a filter assembly (200) and a camera stand (300); The filter assembly (200) is fixed inside the water tank (110) on the side adjacent to the observation port (111); The filter assembly (200) includes a heating rod (210), a circulation pump (220), a filter (230), and a delivery pipe (240); The heating rod (210), the circulation pump (220) and the filter (230) are all fixed on one side inside the water tank (110), and the input end of the circulation pump (220) extends into the bottom of the sieve plate (180); The delivery pipe (240) is connected to the output end of the circulation pump (220), and the end of the delivery pipe (240) away from the circulation pump (220) is connected to the input end of the filter (230); The filter (230) and its output are both located above the water level in the water tank (110); A camera stand (300) is set on one side of the observation board (120). The camera stand (300) is used to place a mobile phone, an infrared camera or a natural light camera to record and observe the movement trajectory of the fish. The mobile phone, infrared camera, and natural light camera recorded videos in MP4, AVI, and MOV formats, respectively.

5. The apparatus for studying the diving behavior of zebrafish as described in claim 3, characterized in that, The research component (100) also includes protective component one (160) and protective component two (170); Protective component one (160) includes airbag one (161) and air pump one (163), and protective component two (170) includes airbag two (171), air pump two (173) and waterproof box (174). Airbag 1 (161) is C-shaped and is fixed inside the water tank (110) and on the side of the observation plate (120); The C-shaped opening of airbag 1 (161) faces the push plate (150). The side of the push plate (150) is in close contact with the airbag (161); Air pump 1 (163) is fixed outside the water tank (110), and the output end of air pump 1 (163) is connected to air bag 1 (161); Airbag 2 (171) is fixed on the side of push plate (150) away from filter assembly (200), and waterproof box (174) is fixed on the side of push plate (150) close to filter assembly (200). The upper end of waterproof box (174) is open and the upper end of waterproof box (174) is located above the horizontal plane inside water tank (110). Air pump 2 (173) is fixed inside the waterproof box (174), and the output end of air pump 2 (173) is connected to airbag 2 (171).

6. The apparatus for studying the diving behavior of zebrafish as described in claim 5, characterized in that, Both airbag one (161) and airbag two (171) are made of liquid silicone rubber; Airbag 1 (161) and airbag 2 (171) are above the water level inside the water tank (110); In the initial state, airbag one (161) and airbag two (171) are in a fully contracted state.

7. The apparatus for studying the diving behavior of zebrafish as described in claim 6, characterized in that, Protective component one (160) includes separator membrane one (162), and protective component two (170) includes separator membrane two (172). The first separator (162) is C-shaped and is fixed inside the first airbag (161). There are multiple first separators (162) and they are evenly spaced from top to bottom to divide the first airbag (161) into multiple separate expansion cavities. The number of air pumps (163) is consistent with the number of individual expansion cavities in airbags (161) separated by the separator membrane (162), and they correspond one-to-one. The output end of air pumps (163) is connected to the individual expansion cavities in airbags (161) separated by the separator membrane (162). Separating membrane two (172) is fixed inside airbag two (171). There are multiple separating membranes two (172), which are evenly spaced from top to bottom, dividing airbag two (171) into multiple separate expansion cavities. The number of air pumps (173) is consistent with the number of individual expansion cavities in airbags (171) separated by the separator membrane (172), and they correspond one-to-one. The output end of air pumps (173) is connected to the individual expansion cavities in airbags (171) separated by the separator membrane (172). Separator membrane 1 (162) and separator membrane 2 (172) are both made of liquid silicone rubber.

Images