A nucleic acid synthesis module and system

Abstract

The application provides a nucleic acid synthesis module and system, and relates to the technical field of nucleic acid synthesis. The module comprises an upper cover, a lower cover, a first sealing element and a synthesis chip. The synthesis reaction zone in the synthesis chip faces the first sealing element. The upper cover comprises a first substrate and a first positioning portion, and the first positioning portion is arranged at the bottom of the first substrate. The lower cover comprises a second substrate and a second positioning portion, and the second positioning portion is arranged at the top of the second substrate. The upper cover and the lower cover are connected to form a containing cavity, and the containing cavity is used for containing the first sealing element and the synthesis reaction zone in the synthesis chip. The first positioning portion and the second positioning portion form a male-female structure to fix the positions of the synthesis chip and the first sealing element, and press the synthesis chip and the first sealing element after locking to form a reaction cavity. The lower cover is provided with an inlet flow channel and an outlet flow channel, and the inlet flow channel and the outlet flow channel are both in communication with the reaction cavity, and the synthesis reaction zone is located in the reaction cavity. The synthesis efficiency and precision of the nucleic acid synthesis module are greatly improved.

Description

Technical Field

[0001] This application relates to the field of nucleic acid synthesis technology, and more specifically, to a nucleic acid synthesis module and system. Background Technology

[0002] Nucleic acid synthesis technology is a fundamental tool in synthetic biology and genetic engineering. Traditional nucleic acid synthesis methods are based on phosphoramide chemical solid-phase synthesis, which, while reliable, has limitations in terms of high throughput and cost efficiency. With technological advancements, chip-based electrochemical nucleic acid synthesis technology has gradually emerged, enabling large-scale parallel nucleic acid sequence synthesis through the miniaturization and integration of reaction modules.

[0003] However, existing chip-based nucleic acid synthesis modules have poor sealing properties, which can easily lead to reagent leakage and consequently reduce nucleic acid synthesis efficiency. Furthermore, the traditional limiting structures in existing nucleic acid synthesis modules make it difficult to precisely adjust the height of the reaction chamber, resulting in uneven reagent distribution and affecting synthesis accuracy.

[0004] In summary, improving the synthesis efficiency and accuracy of nucleic acid synthesis modules is a technical problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0005] The purpose of this application is to provide a nucleic acid synthesis module and system to improve the synthesis efficiency and accuracy of the nucleic acid synthesis module.

[0006] To achieve the above objectives, the technical solution adopted in this application is as follows: On the one hand, this application provides a nucleic acid synthesis module, including: an upper cover, a lower cover, a first sealing element, and a synthesis chip; The synthesized chip includes a synthesis reaction region facing the first sealing element; the upper cover includes a first substrate and a first positioning part, the first positioning part being disposed at the bottom of the first substrate; the lower cover includes a second substrate and a second positioning part, the second positioning part being disposed at the top of the second substrate. The upper cover and the lower cover are connected to form a receiving cavity, which is used to receive the first sealing member and the synthesis reaction zone in the synthesis chip; the first positioning part and the second positioning part are combined to form a matching male and female structure to fix the position of the synthesis chip and the first sealing member, and after locking, the synthesis chip and the first sealing member are squeezed to form a reaction cavity; The lower cover is provided with an inlet channel and an outlet channel, both of which are connected to the reaction chamber, and the synthesis reaction zone is located within the reaction chamber.

[0007] Furthermore, the bottom of the lower cover is provided with a first liquid inlet and a first liquid outlet, and the top of the lower cover is provided with a second liquid inlet, a second liquid outlet, and a first limiting groove that matches the first sealing element; The first sealing element is disposed in the first limiting groove, the first liquid inlet hole and the second liquid inlet hole are connected to form the liquid inlet channel, and the first liquid outlet hole and the second liquid outlet hole are connected to form the liquid outlet channel; The reaction chamber is located inside the first sealing member, the synthesis reaction zone serves as the top of the reaction chamber, and the top of the lower cover containing the second liquid inlet and the second liquid outlet serves as the bottom of the reaction chamber; Synthetic reagents are injected into or discharged into the reaction chamber through the inlet and outlet channels to perform in-situ synthesis of nucleic acids in the synthesis reaction zone.

[0008] Furthermore, the first positioning part includes a first protrusion structure, and the second positioning part includes a second protrusion structure and a third protrusion structure; The first protrusion structure is disposed at the middle position of the bottom of the first substrate, and the two sides of the first protrusion structure form a first recessed structure and a second recessed structure with the bottom of the first substrate, respectively. The second protrusion structure and the third protrusion structure are respectively disposed on both sides of the top of the second substrate; the side wall of the second protrusion structure near the third protrusion structure and the side wall of the third protrusion structure near the second protrusion structure form a third recessed structure with the top of the second substrate; and the second liquid inlet hole, the second liquid outlet hole and the first limiting groove are all disposed at the bottom of the third recessed structure; wherein, the height of the first protrusion structure < the height of the second protrusion structure = the height of the third protrusion structure; When the nucleic acid synthesis module is assembled, the first protruding structure and the third recessed structure combine to form the receiving cavity, the second protruding structure and the first recessed structure combine to form a first male-female structure, and the third protruding structure and the second recessed structure combine to form a second male-female structure.

[0009] Furthermore, the nucleic acid synthesis module also includes a first fixture and a second fixture. The first substrate includes a first side and a second side extending in the width direction, and a third side and a fourth side extending in the length direction. The second substrate includes a fifth side and a sixth side extending in the width direction, and a seventh side and an eighth side extending in the length direction. The fifth side of the second substrate is rotatably connected to the first side of the first substrate, the end where the sixth and seventh sides of the second substrate intersect is rotatably connected to one end of the first clamp, and the end where the sixth and eighth sides of the second substrate intersect is rotatably connected to one end of the second clamp. The first positioning part includes a fourth protruding structure, and the second positioning part includes a fourth recessed structure; the fourth protruding structure is disposed at the middle position of the bottom of the first substrate, the fourth recessed structure is disposed at the middle position of the top of the second substrate, and the second liquid inlet, the second liquid outlet and the first limiting groove are all disposed at the bottom of the fourth recessed structure. The first clamp has a fifth recessed structure on the side wall near the seventh side of the first substrate, and the second clamp has a sixth recessed structure on the side wall near the eighth side of the first substrate. When the nucleic acid synthesis module is assembled, the first substrate and the second substrate are parallel, and the fourth protrusion structure and the fourth recess structure are combined to form the receiving cavity; the third side of the first substrate and the seventh side of the second substrate form a fifth protrusion structure, and the fifth protrusion structure and the fifth recess structure are combined to form a third male and female structure; the fourth side of the first substrate and the eighth side of the second substrate form a sixth protrusion structure, and the sixth protrusion structure and the sixth recess structure are combined to form a fourth male and female structure.

[0010] Furthermore, the nucleic acid synthesis module also includes a first roller, a second roller, and a third roller; The first substrate has a first through hole on its first side, and the second substrate has a second through hole on its fifth side corresponding to the first through hole. The first roller passes horizontally through the first through hole and the second through hole so that the first substrate and the second substrate are rotatably connected. The second substrate has a third through hole at one end where the sixth and seventh sides intersect, and the first clamp has a fourth through hole at one end corresponding to the third through hole. The second roller passes vertically through the fourth through hole and the third through hole so that the first clamp is rotatably connected to the second substrate. The second substrate has a fifth through hole at one end where the sixth side and the eighth side intersect, and the second clamp has a sixth through hole at one end corresponding to the fifth through hole. The third roller passes vertically through the sixth through hole and the fifth through hole so that the second clamp is rotatably connected to the second substrate.

[0011] Furthermore, the nucleic acid synthesis module also includes a glass slide, a second sealing element, and a third sealing element; the glass slide is located between the first sealing element and the lower cover, and the second and third sealing elements are both located between the glass slide and the lower cover; The bottom of the lower cover is provided with a third liquid inlet and a third liquid outlet, and the top of the lower cover is provided with a fourth liquid inlet, a fourth liquid outlet, a second limiting groove that is close to the periphery of the fourth liquid inlet and matches the second sealing element, and a third limiting groove that is close to the periphery of the fourth liquid outlet and matches the third sealing element. The glass slide is provided with a fifth liquid inlet and a fifth liquid outlet, and the top of the glass slide is provided with a fourth limiting groove that matches the first sealing element. The first sealing element, the second sealing element, and the third sealing element are respectively disposed in the second limiting groove, the third limiting groove, and the fourth limiting groove; the third liquid inlet, the fourth liquid inlet, and the fifth liquid inlet are connected to form the liquid inlet channel, and the third liquid outlet, the fourth liquid outlet, and the fifth liquid outlet are connected to form the liquid outlet channel; The reaction chamber is located inside the first sealing member, the synthesis reaction zone serves as the top of the reaction chamber, and the top of the glass slide containing the fifth liquid inlet and the fifth liquid outlet serves as the bottom of the reaction chamber; Synthetic reagents are injected into or discharged into the reaction chamber through the inlet and outlet channels to perform in situ synthesis of nucleic acids on top of the glass slide.

[0012] Furthermore, the first positioning part includes a seventh protrusion structure and an eighth protrusion structure, and the second positioning part includes a ninth protrusion structure and a tenth protrusion structure; The seventh protrusion structure and the eighth protrusion structure are respectively disposed on both sides of the bottom of the first substrate, and the bottom of the seventh protrusion structure is provided with a seventh recess structure that matches the ninth protrusion structure, and the bottom of the eighth protrusion structure is provided with an eighth recess structure that matches the tenth protrusion structure; the sidewall of the seventh protrusion structure near the eighth protrusion structure and the sidewall of the eighth protrusion structure near the seventh protrusion structure form a ninth recess structure with the bottom of the first substrate; The ninth protrusion structure and the tenth protrusion structure are respectively disposed on both sides of the top of the second substrate. The side wall of the ninth protrusion structure near the tenth protrusion structure and the side wall of the tenth protrusion structure near the ninth protrusion structure form a tenth recessed structure with the top of the second substrate. The fourth liquid inlet hole, the fourth liquid outlet hole, the second limiting groove and the third limiting groove are all disposed at the bottom of the tenth recessed structure. When the nucleic acid synthesis module is assembled, the ninth recessed structure and the tenth recessed structure combine to form the receiving cavity, the seventh recessed structure and the ninth protruding structure combine to form the fifth male and female structure, and the eighth recessed structure and the tenth protruding structure combine to form the sixth male and female structure.

[0013] Furthermore, the nucleic acid synthesis module also includes multiple fixing components. The upper cover is provided with multiple first mounting holes, and the lower cover is provided with multiple second mounting holes. The fixing components pass vertically through the first mounting holes and the second mounting holes to fix the upper cover and the lower cover together.

[0014] Furthermore, the synthesized chip also includes a wiring area and a flexible printed circuit. Both the wiring area and the flexible printed circuit are located outside the receiving cavity. The synthesis reaction area is connected to one end of the flexible printed circuit through the wiring area, and the other end of the flexible printed circuit is used to connect to the driving system.

[0015] On the other hand, this application also provides a nucleic acid synthesis system, which includes a nucleic acid synthesis module as described in any of the foregoing embodiments.

[0016] Compared with the prior art, this application has the following advantages: This application provides a nucleic acid synthesis module and system. The nucleic acid synthesis module includes an upper cover, a lower cover, a first sealing element, and a synthesis chip. The synthesis chip includes a synthesis reaction area facing the first sealing element. The upper cover includes a first substrate and a first positioning part, the first positioning part being disposed at the bottom of the first substrate. The lower cover includes a second substrate and a second positioning part, the second positioning part being disposed at the top of the second substrate. The upper and lower covers are connected to form a receiving cavity, which is used to receive the first sealing element and the synthesis reaction area in the synthesis chip. The first and second positioning parts combine to form a matching male-female structure to fix the positions of the synthesis chip and the first sealing element, and after locking, compress the synthesis chip and the first sealing element to form the reaction cavity. The lower cover has an inlet channel and an outlet channel, both of which communicate with the reaction cavity, and the synthesis reaction area is located within the reaction cavity.

[0017] Compared to the traditional limiting structure in existing nucleic acid synthesis modules, this application optimizes the combination of the first positioning part, the second positioning part, the first sealing element, and the synthesis chip. This ensures that the final reaction chamber has high sealing performance, better adapts to internal pressure fluctuations, maintains a stable reaction environment, thereby reducing the risk of reagent leakage and improving synthesis efficiency, safety, and reliability. Furthermore, the male-female structure formed by the first and second positioning parts allows for precise adjustment of the reaction chamber height, ensuring consistent reaction conditions for each synthesis and making it suitable for nucleic acid synthesis needs of varying lengths and complexities. The controllable height design of the reaction chamber improves the uniformity of reagent distribution, reduces dead zones in the reaction area, and significantly enhances the accuracy and efficiency of the synthesis reaction. Attached Figure Description

[0018] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0019] Figure 1 This is one of the exploded schematic diagrams of a nucleic acid synthesis module provided in the embodiments of this application; Figure 2 This is one of the structural schematic diagrams of an upper cover and a lower cover provided in an embodiment of this application; Figure 3 This is a second schematic diagram of the structure of an upper cover and a lower cover provided in an embodiment of this application; Figure 4 One of the side views of a nucleic acid synthesis module provided in an embodiment of this application; Figure 5 This is a second exploded view of a nucleic acid synthesis module provided in an embodiment of this application; Figure 6 This is the third exploded view of a nucleic acid synthesis module provided in the embodiments of this application; Figure 7 This is one of the structural schematic diagrams of a first clamp and a second clamp provided in the embodiments of this application; Figure 8 This is a second schematic diagram of the structure of a first clamp and a second clamp provided in the embodiments of this application; Figure 9 An assembly flowchart of a nucleic acid synthesis module provided in this application embodiment; Figure 10 This is the fourth exploded view of a nucleic acid synthesis module provided in the embodiments of this application; Figure 11 This is the third schematic diagram of the structure of an upper cover and a lower cover provided in an embodiment of this application; Figure 12 A second side view of a nucleic acid synthesis module provided in an embodiment of this application; Figure 13 This is an exploded view of a counter-verification component provided in an embodiment of this application.

[0020] Icons: 10 - Nucleic acid synthesis module; 100 - Top cover; 110 - Hollowed-out window; 120 - First mounting hole; 130 - First substrate; 131 - First through hole; 140 - First positioning part; 141 - First protruding structure; 142 - Fourth protruding structure; 143 - Seventh protruding structure; 144 - Eighth protruding structure; 145 - Seventh recessed structure; 146 - Eighth recessed structure; 151 - First recessed structure; 152 - Second recessed structure; 153 - Ninth recessed structure; 200 - Bottom cover; 210 - Second mounting hole; 220 - Second substrate; 221 - Second through hole; 222 - Third through hole; 223 - Fifth through hole; 230 - Second positioning part; 231 - Second protruding structure; 232 - Third protruding structure; 233 - Fourth recessed structure; 234 - Ninth protruding structure; 235 - Tenth protruding structure; 241 - Third recessed structure; 242 - Tenth recessed structure; 251 - First liquid inlet hole; 252 - First liquid outlet hole; 253 - Third liquid inlet hole; 25 4-Third liquid outlet; 261-Second liquid inlet; 262-Second liquid outlet; 263-First limiting groove; 264-Fourth liquid inlet; 265-Fourth liquid outlet; 266-Second limiting groove; 267-Third limiting groove; 310-First sealing element; 320-Second sealing element; 330-Third sealing element; 400-Synthetic chip; 410-Synthetic reaction zone; 420-Wire routing area; 430-Flexible printed circuit; 500-Receiving cavity; 610-First clamp; 611-The Five recessed structures; 612-Fourth through hole; 620-Second clamp; 621-Sixth recessed structure; 622-Sixth through hole; 710-Fifth protruding structure; 720-Sixth protruding structure; 810-Fixing component; 820-First roller; 830-Second roller; 900-Slide; 911-Fifth liquid inlet hole; 912-Fifth liquid outlet hole; 913-Fourth limiting groove; 20-Verification component on opposite side; 21-Height limiter; 22-Adhesive component; 23-Liquid inlet interface; 24-Liquid outlet interface. Detailed Implementation

[0021] As used herein, the following terms are intended to have the meanings set forth below. It should be understood that these definitions are provided to aid in understanding this application and are not intended to be limiting. Unless the context clearly provides otherwise, the singular forms “a,” “an,” and “the” include plural referents.

[0022] When a term is not specifically defined, it shall be given the common and customary meaning as understood by one of ordinary skill in the art at the time of filing of this application. In the event of any conflict between any generally accepted definition in the art and the definition provided herein, the definition provided herein shall prevail.

[0023] Various aspects of this application are presented in scope format. It should be understood that the scope format is merely for convenience and brevity and should not be construed as a rigid limitation on the scope of this application. Therefore, the scope description should be considered as having specifically disclosed all possible sub-scopes and common individual values ​​within that scope.

[0024] Unless otherwise stated, implementation of this application may be carried out using conventional techniques of organic chemistry, polymer technology, molecular biology (including recombinant techniques), cell biology, biochemistry, and immunology, which are within the scope of the art. Such conventional techniques include polymer array synthesis, hybridization, ligation, and detection of hybridization using labels. Specific descriptions of suitable techniques can be obtained by referring to the examples below. However, other equivalent conventional procedures may of course be used. Such conventional techniques can be found in standard laboratory manuals.

[0025] The term "nucleic acid" can include any polymer or oligomer of pyrimidine and purine bases, preferably cytosine, thymine, and uracil, and adenine and guanine, respectively. In fact, this application considers any deoxyribonucleotide, ribonucleotide, or peptide nucleic acid component, and any chemical variants thereof, such as methylated, hydroxymethylated, or glycosylated forms of these bases. The polymer or oligomer can be heterogeneous or homogeneous in composition and can be isolated from naturally occurring sources or can be artificially or synthetically produced. Furthermore, nucleic acids can be DNA or RNA, or mixtures thereof, and can exist permanently or intermittently in single-stranded or double-stranded form, including homoduplex, heteroduplex, and hybrid states.

[0026] The term "synthetic chip" specifically refers to any solid, semi-solid, or composite substrate used for the parallel, site-specific synthesis of oligonucleotides or longer nucleic acid molecules. In fact, this application considers any form of substrate, whether its material is organic (such as various polymers, hydrogels) or inorganic (such as glass, quartz, silicon, ceramics, or metals), and its morphology can be rigid, flexible, porous, or three-dimensional. Furthermore, the substrate surface can be planar or contain microscopic or nanoscopic topologies formed by any means such as etching, molding, printing, or deposition, such as micropores, micropillars, nanopores, or microfluidic channels. The substrate can be made of various materials, such as traditional glass slides, glass plates, or silicon wafers that can be processed using semiconductor techniques. Its surface has multiple discrete reaction sites, which can be defined by physical structures (such as micropores or micropillars) directly formed on the substrate (e.g., on a silicon wafer with an etched structure); or, these reaction sites can be defined and independently addressed by functional regions, for example, on a glass or silicon wafer with electrodes, where each electrode can be precisely controlled by underlying CMOS or TFT semiconductor circuitry, thereby creating a highly localized chemical environment to trigger specific steps in the synthesis reaction. Furthermore, the nucleic acid molecules synthesized on this chip can be DNA, RNA, or any chemical variant or analogue thereof, and their synthesis process can be based on any iterative chemical, electrochemical, biochemical, photochemical, and enzymatic methods, ultimately forming a high-density, sequence-programmable oligonucleotide array on the chip.

[0027] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0028] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0029] In the description of this application, it should be noted that relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. The term "connection" should be interpreted broadly, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection or an indirect connection through an intermediate medium. The following detailed description of some embodiments of this application is provided in conjunction with the accompanying drawings. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0030] As described in the background section, existing nucleic acid synthesis modules have poor sealing properties, which can easily lead to reagent leakage and consequently reduce nucleic acid synthesis efficiency. Furthermore, the traditional limiting structures in existing nucleic acid synthesis modules make it difficult to precisely adjust the height of the reaction chamber, resulting in uneven reagent distribution and affecting synthesis accuracy. Therefore, how to improve the synthesis efficiency and accuracy of nucleic acid synthesis modules is a technical problem that urgently needs to be solved by those skilled in the art.

[0031] To resolve the above technical issues, please refer to Figure 1 This application provides a nucleic acid synthesis module 10, including: an upper cover 100, a lower cover 200, a first sealing member 310, and a synthesis chip 400. The synthesis chip 400 is located between the upper cover 100 and the lower cover 200, and the first sealing member 310 is located between the synthesis chip 400 and the lower cover 200.

[0032] The synthesized chip 400 includes a synthesis reaction region 410 facing the first seal 310. The upper cover 100 includes a first substrate 130 and a first positioning portion 140, with the first positioning portion 140 disposed at the bottom of the first substrate 130. The lower cover 200 includes a second substrate 220 and a second positioning portion 230, with the second positioning portion 230 disposed at the top of the second substrate 220.

[0033] The upper cover 100 and the lower cover 200 are connected to form a receiving cavity, which is used to receive the first seal 310 and the synthesis reaction zone 410 in the synthesis chip 400. The first positioning part 140 and the second positioning part 230 are combined to form a matching male and female structure to fix the position of the synthesis chip 400 and the first seal 310, and after locking, the synthesis chip 400 and the first seal 310 are pressed together to form the reaction cavity.

[0034] The lower cover 200 is equipped with an inlet channel and an outlet channel, both of which are connected to the reaction chamber, and the synthesis reaction zone 410 is located within the reaction chamber. Relevant synthetic reagents are injected into or discharged from the reaction chamber through the inlet and outlet channels, thereby enabling high-throughput synthesis of nucleic acids. The target product is an oligonucleotide.

[0035] Optionally, both the upper cover 100 and the lower cover 200 are made of a rigid material (e.g., PEEK) to ensure that minor deformations of the upper cover 100 and the lower cover 200 under stress can be ignored. In addition, the upper cover 100 has a perforated viewing window 110, which allows observation of the internal liquid level or flow status when the nucleic acid synthesis module 10 is installed and begins to work.

[0036] Based on the above design, this application forms an upper cover 100 by providing a first positioning part 140 at the bottom of the first substrate 130, and a lower cover 200 by providing a second positioning part 230 at the top of the second substrate 220. When the nucleic acid synthesis module 10 is assembled, the upper cover 100, the synthesis chip 400, the first sealing member 310, and the lower cover 200 are sequentially assembled and tightly joined together. The male-female structure formed by the first positioning part 140 and the second positioning part 230 provides a receiving cavity of ideal height, and the first sealing member 310 and the synthesis reaction zone 410 in the synthesis chip 400 are confined within this receiving cavity. Under the pressure of the receiving cavity, the synthesis chip 400 and the first sealing member 310 are squeezed to form a reaction cavity with high sealing performance, uniform cavity height, and controllable performance.

[0037] Compared to the traditional limiting structure in existing nucleic acid synthesis modules, this application optimizes the combination of the first positioning part 140, the second positioning part 230, the first sealing element 310, and the synthesis chip 400. This ensures that the final reaction chamber has high sealing performance, better adapts to pressure fluctuations within the chamber, maintains a stable reaction environment, thereby reducing the risk of reagent leakage and improving synthesis efficiency, safety, and reliability. Furthermore, the male-female structure formed by the first positioning part 140 and the second positioning part 230 allows for precise adjustment of the reaction chamber height, ensuring consistent reaction conditions for each synthesis and making it suitable for nucleic acid synthesis needs of varying lengths and complexities. The controllable height design of the reaction chamber improves the uniformity of reagent distribution, reduces dead zones in the reaction area, and significantly enhances the accuracy and efficiency of the synthesis reaction.

[0038] Furthermore, the nucleic acid synthesis module 10 also includes multiple fasteners 810. The upper cover 100 has multiple first mounting holes 120, and the lower cover 200 has multiple second mounting holes 210. The fasteners 810 pass vertically through the first mounting holes 120 and the second mounting holes 210 to securely connect the upper cover 100 and the lower cover 200. Optionally, the fasteners 810 include screws and nuts.

[0039] In one alternative implementation, please refer to Figures 1 to 3 The bottom of the lower cover 200 is provided with a first liquid inlet 251 and a first liquid outlet 252, and the top of the lower cover 200 is provided with a second liquid inlet 261, a second liquid outlet 262, and a first limiting groove 263 that matches the first sealing member 310.

[0040] The first sealing element 310 is disposed in the first limiting groove 263, the first liquid inlet hole 251 and the second liquid inlet hole 261 are connected to form a liquid inlet channel, and the first liquid outlet hole 252 and the second liquid outlet hole 262 are connected to form a liquid outlet channel.

[0041] The reaction chamber is located inside the first seal 310, the synthesis reaction zone 410 serves as the top of the reaction chamber, and the top of the lower cover 200, which contains the second liquid inlet 261 and the second liquid outlet 262, serves as the bottom of the reaction chamber.

[0042] Synthetic reagents are injected into or discharged into the reaction chamber through the inlet and outlet channels to perform in-situ synthesis of nucleic acids in the synthesis reaction zone 410.

[0043] Optionally, the composite chip 400 further includes a wiring area 420 and a flexible printed circuit (FPC) 430. Both the wiring area 420 and the flexible printed circuit 430 are located outside the receiving cavity, while the composite reaction area 410 is located inside the receiving cavity. The composite reaction area 410 is connected to one end of the flexible printed circuit 430 via the wiring area 420, and the other end of the flexible printed circuit 430 is used to connect to the driving system.

[0044] To better understand the combination of the first positioning part 140 and the second positioning part 230, please refer to the following as an optional implementation: Figures 2 to 4 The first positioning part 140 includes a first protrusion structure 141, and the second positioning part 230 includes a second protrusion structure 231 and a third protrusion structure 232.

[0045] Optionally, the lengths of the first protrusion structure 141, the second protrusion structure 231, the third protrusion structure 232, the first substrate 130, and the second substrate 220 are all the same, and the width of the first substrate 130 is the same as the width of the second substrate 220. Furthermore, the width of the first protrusion structure 141 = the width of the second substrate 220 - the width of the second protrusion structure 231 - the width of the third protrusion structure 232.

[0046] The first protrusion structure 141 is disposed at the middle position of the bottom of the first substrate 130, and the two sides of the first protrusion structure 141 form a first recessed structure 151 and a second recessed structure 152 with the bottom of the first substrate 130, respectively.

[0047] The second protrusion structure 231 and the third protrusion structure 232 are respectively disposed on both sides of the top of the second substrate 220. The second protrusion structure 231 near the sidewall of the third protrusion structure 232, and the third protrusion structure 232 near the sidewall of the second protrusion structure 231, together with the top of the second substrate 220, form a third recessed structure 241. Furthermore, the second liquid inlet hole 261, the second liquid outlet hole 262, and the first limiting groove 263 are all disposed at the bottom of the third recessed structure 241.

[0048] like Figure 4 As shown, the height a of the first protrusion 141 is less than the height b of the second protrusion 231 and equal to the height c of the third protrusion 232.

[0049] Based on the above design, when the nucleic acid synthesis module 10 is assembled, the first protruding structure 141 and the third recessed structure 241 combine to form a receiving cavity 500, the second protruding structure 231 and the first recessed structure 151 combine to form a first male-female structure, and the third protruding structure 232 and the second recessed structure 152 combine to form a second male-female structure. That is, the two sides of the bottom of the upper cover 100 and the two sides of the top of the lower cover 200 make hard contact, forming matching male-female structures respectively. The height of the receiving cavity 500 can be adjusted by adjusting the height of the first protruding structure 141, the second protruding structure 231 and the third protruding structure 232. Optionally, the height of the receiving cavity 500 can be designed to be 100~200μm to facilitate stable clamping of the synthesis chip 400. Under the pressure of the receiving cavity 500, the synthesis chip 400 and the first sealing member 310 are squeezed to form a reaction cavity with high sealing performance, uniform cavity height and controllable performance, thereby enabling in-situ synthesis of nucleic acids in the synthesis reaction zone 410 within the reaction cavity.

[0050] As can be seen, the assembly method of the above-mentioned nucleic acid synthesis module 10 is as follows: multiple first mounting holes 120 are opened on the edge of the upper cover 100, and multiple second mounting holes 210 are opened on the edge of the lower cover 200. A fastener 810 is then vertically passed through the first mounting holes 120 and the second mounting holes 210, thereby sequentially assembling the upper cover 100, the synthesis chip 400, the first sealing element 310, and the lower cover 200 in the vertical direction. This vertical fixing method may result in different fixing forces due to varying torques of the multiple fasteners 810, ultimately leading to uneven reactions during the synthesis process and affecting the synthesis accuracy.

[0051] In view of this, please refer to Figure 5As an alternative implementation, the nucleic acid synthesis module 10 further includes a first clamp 610 and a second clamp 620. Furthermore, the first substrate 130 includes a first side and a second side extending in the width direction, and a third side and a fourth side extending in the length direction. The second substrate 220 includes a fifth side and a sixth side extending in the width direction, and a seventh side and an eighth side extending in the length direction.

[0052] The fifth side of the second substrate 220 is rotatably connected to the first side of the first substrate 130, and the end where the sixth and seventh sides of the second substrate 220 intersect (i.e. Figure 5 The A end of the second substrate 220 is rotatably connected to one end of the first clamp 610, and the end where the sixth and eighth sides of the second substrate 220 intersect (i.e., the end of the first clamp 610) is rotatably connected to one end of the second clamp 610. Figure 5 The B end of the fixture is rotatably connected to one end of the second fixture 620.

[0053] Specifically, please refer to Figure 6 The nucleic acid synthesis module 10 also includes a first roller 820, a second roller 830, and a third roller (not shown in the figure). The first substrate 130 has a first through-hole 131 on its first side, and the second substrate 220 has a second through-hole 221 corresponding to the first through-hole 131 on its fifth side. The first roller 820 passes horizontally through the first through-hole 131 and the second through-hole 221, so that the first substrate 130 and the second substrate 220 are rotatably connected.

[0054] The second substrate 220 has a third through hole 222 at one end where the sixth and seventh sides intersect. The first clamp 610 has a fourth through hole 612 at one end that corresponds to the third through hole 222. The second roller 830 passes vertically through the fourth through hole 612 and the third through hole 222 so that the first clamp 610 is rotatably connected to the second substrate 220.

[0055] The second substrate 220 has a fifth through hole 223 at one end where the sixth side intersects the eighth side, and the second clamp 620 has a sixth through hole 622 at one end that corresponds to the fifth through hole 223. The third roller passes vertically through the sixth through hole 622 and the fifth through hole 223 so that the second clamp 620 is rotatably connected to the second substrate 220.

[0056] Further, please refer to Figures 5 to 8 The first positioning part includes a fourth protruding structure 142, and the second positioning part includes a fourth recessed structure 233. The fourth protruding structure 142 is located at the middle position of the bottom of the first substrate 130, and the fourth recessed structure 233 is located at the middle position of the top of the second substrate 220. The second liquid inlet hole 261, the second liquid outlet hole 262, and the first limiting groove 263 are all located at the bottom of the fourth recessed structure 233.

[0057] like Figure 7 and Figure 8As shown, the first clamp 610 has a fifth recessed structure 611 on the side wall near the seventh side of the first substrate 130, and the second clamp 620 has a sixth recessed structure 621 on the side wall near the eighth side of the first substrate 130.

[0058] When the nucleic acid synthesis module 10 is assembled, the first substrate 130 and the second substrate 220 are parallel, and the fourth protrusion structure 142 and the fourth recessed structure 233 combine to form a receiving cavity 500. At this time, the third side of the first substrate 130 and the seventh side of the second substrate 220 form a fifth protrusion structure 710 (e.g., Figure 7 As shown), the fifth protruding structure 710 and the fifth recessed structure 611 combine to form the third male-female structure. The fourth side of the first substrate 130 and the eighth side of the second substrate 220 form the sixth protruding structure 720 (as shown). Figure 8 As shown), the sixth protruding structure 720 and the sixth recessed structure 621 combine to form the fourth male and female structure.

[0059] To better understand, the following will be combined with... Figure 9 The assembly process of the above-mentioned nucleic acid synthesis module 10 is described.

[0060] During the assembly preparation stage, such as Figure 9 As shown in (a), the angle between the first substrate 130 and the second substrate 220 is greater than 0°, and the angle between the first clamp 610 and the second substrate 220 is greater than 0°, and the angle between the second clamp 620 and the second substrate 220 is greater than 0°, so as to facilitate the mounting of the composite chip 400 and the first seal 310.

[0061] After the composite chip 400 and the first seal 310 are installed, as follows Figure 9 As shown in (b), the first substrate 130 is rotated to a position parallel to the second substrate 220. At this time, the fourth protrusion structure 142 at the bottom of the first substrate 130 and the fourth recess structure 233 at the top of the second substrate 220 combine to form a receiving cavity 500.

[0062] Finally, as Figure 9 As shown in (c), the first clamp 610 rotates toward the seventh side of the second substrate 220 and combines with the third side of the first substrate 130 and the seventh side of the second substrate 220 to form a third male-female structure. The second clamp 620 rotates toward the eighth side of the second substrate 220 and combines with the fourth side of the first substrate 130 and the eighth side of the second substrate 220 to form a fourth male-female structure. At this time, the nucleic acid synthesis module 10 is assembled.

[0063] Based on the above design, a cavity 500 of ideal height is formed by combining the fourth protrusion structure 142 and the fourth recess structure 233. The synthesis chip 400 and the first sealing member 310 are squeezed evenly from left to right by the third male and female structure and the fourth male and female structure, and finally a reaction cavity with high sealing performance and high controllability is formed for in-situ synthesis of nucleic acids.

[0064] Compared to the vertical fixing method of the fastener 810, the clip-on assembly design simplifies the assembly process of the nucleic acid synthesis module 10. More importantly, the double-sided clamps (i.e., the first clamp 610 and the second clamp 620) provide rigid fixation, resulting in more uniform and stable fixing force. This uniform and stable double-sided clamping force ensures consistent sealing of the reaction chamber, thereby effectively improving the reaction uniformity and synthesis accuracy of nucleic acid synthesis.

[0065] As can be seen, this application provides two types of nucleic acid synthesis modules 10 for in-situ nucleic acid synthesis. To alleviate spatial crosstalk issues in in-situ synthesis and meet the needs of different application scenarios, for non-in-situ nucleic acid synthesis, please refer to [link to relevant documentation]. Figure 10 and Figure 11 In another alternative embodiment, the nucleic acid synthesis module 10 further includes a glass slide 900, a second seal 320, and a third seal 330.

[0066] The slide 900 is located between the first seal 310 and the lower cover 200, and the second seal 320 and the third seal 330 are both located between the slide 900 and the lower cover 200. Optionally, the first seal 310, the second seal 320 and the third seal 330 are all O-rings.

[0067] The bottom of the lower cover 200 is provided with a third liquid inlet hole 253 and a third liquid outlet hole 254. The top of the lower cover 200 is provided with a fourth liquid inlet hole 264, a fourth liquid outlet hole 265, a second limiting groove 266 that is close to the periphery of the fourth liquid inlet hole 264 and matches the second sealing member 320, and a third limiting groove 267 that is close to the periphery of the fourth liquid outlet hole 265 and matches the third sealing member 330.

[0068] The slide 900 is provided with a fifth liquid inlet hole 911 and a fifth liquid outlet hole 912, and the top of the slide 900 is provided with a fourth limiting groove 913 that matches the first sealing member 310.

[0069] The first sealing element 310, the second sealing element 320 and the third sealing element 330 are respectively disposed in the second limiting groove 266, the third limiting groove 267 and the fourth limiting groove 913.

[0070] The third liquid inlet hole 253, the fourth liquid inlet hole 264 and the fifth liquid inlet hole 911 are connected to form a liquid inlet channel, and the third liquid outlet hole 254, the fourth liquid outlet hole 265 and the fifth liquid outlet hole 912 are connected to form a liquid outlet channel.

[0071] The reaction chamber is located inside the first sealing member 310, the synthesis reaction zone 410 serves as the top of the reaction chamber, and the top of the glass slide 900 containing the fifth liquid inlet 911 and the fifth liquid outlet 912 serves as the bottom of the reaction chamber.

[0072] Synthetic reagents are injected or discharged into the reaction chamber through the inlet and outlet channels to perform in situ synthesis of nucleic acids on top of the slide 900.

[0073] As can be seen, the ex-situ synthesis of nucleic acids takes place on the opposite side of the synthesis reaction zone 410, i.e., on top of the slide 900. At this time, the synthesis reaction zone 410 only provides the reaction environment and does not synthesize nucleic acids.

[0074] Furthermore, since the reaction chamber for in-situ nucleic acid synthesis is composed of a synthesis chip 400, a first sealing element 310, and a glass slide 900, in order to prevent reagent leakage between the lower cover 200 and the glass slide 900, this application provides a second limiting groove 266 and a second sealing element 320 around the fourth liquid inlet 264, and a third limiting groove 267 and a third sealing element 330 around the fourth liquid outlet 265. This prevents the synthesis reagent from leaking out of the reaction chamber from the fourth liquid inlet 264 and / or the fourth liquid outlet 265, further improving the sealing performance of the nucleic acid synthesis module 10.

[0075] Optionally, the nucleic acid synthesis module 10 also includes multiple fasteners 810, with the upper cover 100 having multiple first mounting holes 120 and the lower cover 200 having multiple second mounting holes 210. The fasteners 810 pass vertically through the first mounting holes 120 and the second mounting holes 210 to fix the upper cover 100 and the lower cover 200 together.

[0076] To better understand the combination of the first and second positioning parts in the nucleic acid synthesis module 10 described above, please refer to... Figures 10 to 12 In one optional embodiment, the first positioning part includes a seventh protrusion structure 143 and an eighth protrusion structure 144, and the second positioning part includes a ninth protrusion structure 234 and a tenth protrusion structure 235.

[0077] The seventh protrusion structure 143 and the eighth protrusion structure 144 are respectively disposed on both sides of the bottom of the first substrate 130. The bottom center of the seventh protrusion structure 143 is provided with a seventh recess structure 145 that matches the ninth protrusion structure 234, and the bottom center of the eighth protrusion structure 144 is provided with an eighth recess structure 146 that matches the tenth protrusion structure 235. The seventh protrusion structure 143 near the sidewall of the eighth protrusion structure 144 and the eighth protrusion structure 144 near the sidewall of the seventh protrusion structure 143 form a ninth recess structure 153 with the bottom of the first substrate 130.

[0078] The ninth protrusion structure 234 and the tenth protrusion structure 235 are respectively disposed on both sides of the top of the second substrate 220. The ninth protrusion structure 234 near the sidewall of the tenth protrusion structure 235, and the tenth protrusion structure 235 near the sidewall of the ninth protrusion structure 234, together with the top of the second substrate 220, form a tenth recessed structure 242. Furthermore, the fourth liquid inlet hole 264, the fourth liquid outlet hole 265, the second limiting groove 266, and the third limiting groove 267 are all disposed at the bottom of the tenth recessed structure 242.

[0079] When the nucleic acid synthesis module 10 is assembled, the ninth recessed structure 153 and the tenth recessed structure 242 combine to form the receiving cavity 500, the seventh recessed structure 145 and the ninth protruding structure 234 combine to form the fifth male-female structure, and the eighth recessed structure 146 and the tenth protruding structure 235 combine to form the sixth male-female structure. That is, the two sides of the bottom of the upper cover 100 and the two sides of the top of the lower cover 200 make hard contact, forming matching male-female structures respectively. The height of the receiving cavity 500 can be adjusted by adjusting the height of the seventh protruding structure 143 and the eighth protruding structure 144. Optionally, the height of the receiving cavity 500 can be designed to be 200~300μm to facilitate stable clamping of the synthesis chip 400 and the glass slide 900. Under the pressure of the accommodating cavity 500, the first sealing element 310, the synthesis chip 400, and the glass slide 900 are squeezed to form a reaction cavity with high sealing performance, uniform cavity height, and controllable capacity, thereby enabling in-situ synthesis of nucleic acids on top of the glass slide 900 within the reaction cavity.

[0080] Furthermore, for non-in situ nucleic acid synthesis, to ensure a more standardized design for nucleic acid synthesis module 10, please refer to [link to relevant documentation]. Figure 13 This application embodiment also provides a counter-side verification component 20, including: a liquid inlet interface 23, a liquid outlet interface 24, a glass slide 900 with a fifth liquid inlet hole 911 and a fifth liquid outlet hole 912, a height limiter 21, an adhesive 22, and a composite chip 400.

[0081] The synthesis reaction zone 410 in the synthesis chip 400 faces the glass slide 900. The synthesis reaction zone 410 is bonded to the glass slide 900 by the adhesive 22. By placing the height limiter 21 between the synthesis reaction zone 410 and the glass slide 900, a reaction chamber with a fixed height and sealed is obtained. Optionally, the height limiter 21 is a frame of uniform thickness, and the adhesive 22 is UV adhesive.

[0082] The fifth liquid inlet 911 and the fifth liquid outlet 912 are both contained in the reaction chamber, and the fifth liquid inlet 911 and the fifth liquid outlet 912 are connected to the liquid inlet interface 23 and the liquid outlet interface 24, respectively, for injecting and discharging the synthesis reagents into the reaction chamber, thereby performing in-situ synthesis of nucleic acids on the glass slide 900 on the opposite side of the synthesis reaction zone 410.

[0083] Understandably, the contralateral verification component 20 is a verification version of the nucleic acid synthesis module 10. After the functional verification of non-in situ nucleic acid synthesis is completed through the contralateral verification component 20, a more standardized nucleic acid synthesis module 10 can be designed.

[0084] Furthermore, this application embodiment also provides a nucleic acid synthesis system, which includes a nucleic acid synthesis module 10 as described in any of the foregoing embodiments.

[0085] In summary, this application provides a nucleic acid synthesis module and system. The nucleic acid synthesis module includes an upper cover, a lower cover, a first sealing element, and a synthesis chip. The synthesis chip includes a synthesis reaction area facing the first sealing element. The upper cover includes a first substrate and a first positioning part, the first positioning part being disposed at the bottom of the first substrate. The lower cover includes a second substrate and a second positioning part, the second positioning part being disposed at the top of the second substrate. The upper and lower covers are connected to form a receiving cavity, which is used to receive the first sealing element and the synthesis reaction area in the synthesis chip. The first and second positioning parts combine to form a matching male-female structure to fix the positions of the synthesis chip and the first sealing element, and after locking, compress the synthesis chip and the first sealing element to form a reaction cavity. The lower cover has an inlet channel and an outlet channel, both of which communicate with the reaction cavity, and the synthesis reaction area is located within the reaction cavity.

[0086] This application optimizes the combination of the first positioning part, the second positioning part, the first sealing element, and the synthesis chip, ensuring that the final reaction chamber has high sealing performance. This allows it to better adapt to pressure fluctuations within the chamber, maintain a stable reaction environment, reduce the risk of reagent leakage, and improve synthesis efficiency, safety, and reliability. Furthermore, the male-female structure formed by the first and second positioning parts allows for precise adjustment of the reaction chamber height, ensuring consistent reaction conditions for each synthesis and making it suitable for nucleic acid synthesis needs of varying lengths and complexities. The controllable height design of the reaction chamber improves the uniformity of reagent distribution, reduces dead zones in the reaction area, and significantly enhances the accuracy and efficiency of the synthesis reaction, promoting the industrial application of high-throughput nucleic acid synthesis.

[0087] Furthermore, this application enhances the assembly flexibility and ease of maintenance of the nucleic acid synthesis module through its compact and modular design. This nucleic acid synthesis module achieves a balance between miniaturization, high throughput, and high precision, meeting the diverse needs of industrialization and laboratory settings.

[0088] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

[0089] It will be apparent to those skilled in the art that this application is not limited to the details of the exemplary embodiments described above, and that this application can be implemented in other specific forms without departing from the spirit or essential characteristics of this application. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this application is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this application. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A nucleic acid synthesis module, characterized in that, include: Top cover, bottom cover, first seal, and composite chip; The synthesized chip includes a synthesis reaction region facing the first sealing element; the upper cover includes a first substrate and a first positioning part, the first positioning part being disposed at the bottom of the first substrate; the lower cover includes a second substrate and a second positioning part, the second positioning part being disposed at the top of the second substrate. The upper cover and the lower cover are connected to form a receiving cavity, which is used to accommodate the first sealing element and the synthesis reaction zone in the synthesis chip; The first positioning part and the second positioning part combine to form a matching male and female structure to fix the position of the synthetic chip and the first sealing member, and after locking, the synthetic chip and the first sealing member are squeezed to form a reaction chamber; The lower cover is provided with an inlet channel and an outlet channel, both of which are connected to the reaction chamber, and the synthesis reaction zone is located within the reaction chamber.

2. The nucleic acid synthesis module according to claim 1, characterized in that, The bottom of the lower cover is provided with a first liquid inlet and a first liquid outlet, and the top of the lower cover is provided with a second liquid inlet, a second liquid outlet, and a first limiting groove that matches the first sealing element. The first sealing element is disposed in the first limiting groove, the first liquid inlet hole and the second liquid inlet hole are connected to form the liquid inlet channel, and the first liquid outlet hole and the second liquid outlet hole are connected to form the liquid outlet channel; The reaction chamber is located inside the first sealing member, the synthesis reaction zone serves as the top of the reaction chamber, and the top of the lower cover containing the second liquid inlet and the second liquid outlet serves as the bottom of the reaction chamber; Synthetic reagents are injected into or discharged into the reaction chamber through the inlet and outlet channels to perform in-situ synthesis of nucleic acids in the synthesis reaction zone.

3. The nucleic acid synthesis module according to claim 2, characterized in that, The first positioning part includes a first protrusion structure, and the second positioning part includes a second protrusion structure and a third protrusion structure; The first protrusion structure is disposed at the middle position of the bottom of the first substrate, and the two sides of the first protrusion structure form a first recessed structure and a second recessed structure with the bottom of the first substrate, respectively. The second protrusion structure and the third protrusion structure are respectively disposed on both sides of the top of the second substrate; The second protruding structure is close to the side wall of the third protruding structure, and the third protruding structure is close to the side wall of the second protruding structure and forms a third recessed structure with the top of the second substrate; and the second liquid inlet, the second liquid outlet and the first limiting groove are all disposed at the bottom of the third recessed structure; wherein, the height of the first protruding structure < the height of the second protruding structure = the height of the third protruding structure; When the nucleic acid synthesis module is assembled, the first protruding structure and the third recessed structure combine to form the receiving cavity, the second protruding structure and the first recessed structure combine to form a first male-female structure, and the third protruding structure and the second recessed structure combine to form a second male-female structure.

4. The nucleic acid synthesis module according to claim 2, characterized in that, The nucleic acid synthesis module further includes a first fixture and a second fixture. The first substrate includes a first side and a second side extending in the width direction, and a third side and a fourth side extending in the length direction. The second substrate includes a fifth side and a sixth side extending in the width direction, and a seventh side and an eighth side extending in the length direction. The fifth side of the second substrate is rotatably connected to the first side of the first substrate, the end where the sixth and seventh sides of the second substrate intersect is rotatably connected to one end of the first clamp, and the end where the sixth and eighth sides of the second substrate intersect is rotatably connected to one end of the second clamp. The first positioning part includes a fourth protruding structure, and the second positioning part includes a fourth recessed structure; the fourth protruding structure is disposed at the middle position of the bottom of the first substrate, the fourth recessed structure is disposed at the middle position of the top of the second substrate, and the second liquid inlet, the second liquid outlet and the first limiting groove are all disposed at the bottom of the fourth recessed structure. The first clamp has a fifth recessed structure on the side wall near the seventh side of the first substrate, and the second clamp has a sixth recessed structure on the side wall near the eighth side of the first substrate. When the nucleic acid synthesis module is assembled, the first substrate and the second substrate are parallel, and the fourth protrusion structure and the fourth recess structure are combined to form the receiving cavity; the third side of the first substrate and the seventh side of the second substrate form a fifth protrusion structure, and the fifth protrusion structure and the fifth recess structure are combined to form a third male and female structure; the fourth side of the first substrate and the eighth side of the second substrate form a sixth protrusion structure, and the sixth protrusion structure and the sixth recess structure are combined to form a fourth male and female structure.

5. The nucleic acid synthesis module according to claim 4, characterized in that, The nucleic acid synthesis module also includes a first roller, a second roller, and a third roller; The first substrate has a first through hole on its first side, and the second substrate has a second through hole on its fifth side corresponding to the first through hole. The first roller passes horizontally through the first through hole and the second through hole so that the first substrate and the second substrate are rotatably connected. The second substrate has a third through hole at one end where the sixth and seventh sides intersect, and the first clamp has a fourth through hole at one end corresponding to the third through hole. The second roller passes vertically through the fourth through hole and the third through hole so that the first clamp is rotatably connected to the second substrate. The second substrate has a fifth through hole at one end where the sixth side and the eighth side intersect, and the second clamp has a sixth through hole at one end corresponding to the fifth through hole. The third roller passes vertically through the sixth through hole and the fifth through hole so that the second clamp is rotatably connected to the second substrate.

6. The nucleic acid synthesis module according to claim 1, characterized in that, The nucleic acid synthesis module further includes a glass slide, a second sealing element, and a third sealing element; the glass slide is located between the first sealing element and the lower cover, and the second and third sealing elements are both located between the glass slide and the lower cover; The bottom of the lower cover is provided with a third liquid inlet and a third liquid outlet, and the top of the lower cover is provided with a fourth liquid inlet, a fourth liquid outlet, a second limiting groove that is close to the periphery of the fourth liquid inlet and matches the second sealing element, and a third limiting groove that is close to the periphery of the fourth liquid outlet and matches the third sealing element. The glass slide is provided with a fifth liquid inlet and a fifth liquid outlet, and the top of the glass slide is provided with a fourth limiting groove that matches the first sealing element. The first sealing element, the second sealing element, and the third sealing element are respectively disposed in the second limiting groove, the third limiting groove, and the fourth limiting groove; the third liquid inlet, the fourth liquid inlet, and the fifth liquid inlet are connected to form the liquid inlet channel, and the third liquid outlet, the fourth liquid outlet, and the fifth liquid outlet are connected to form the liquid outlet channel; The reaction chamber is located inside the first sealing member, the synthesis reaction zone serves as the top of the reaction chamber, and the top of the glass slide containing the fifth liquid inlet and the fifth liquid outlet serves as the bottom of the reaction chamber; Synthetic reagents are injected into or discharged into the reaction chamber through the inlet and outlet channels to perform in situ synthesis of nucleic acids on top of the glass slide.

7. The nucleic acid synthesis module according to claim 6, characterized in that, The first positioning part includes a seventh protrusion structure and an eighth protrusion structure, and the second positioning part includes a ninth protrusion structure and a tenth protrusion structure; The seventh protrusion structure and the eighth protrusion structure are respectively disposed on both sides of the bottom of the first substrate, and the bottom of the seventh protrusion structure is provided with a seventh recess structure that matches the ninth protrusion structure, and the bottom of the eighth protrusion structure is provided with an eighth recess structure that matches the tenth protrusion structure; the sidewall of the seventh protrusion structure near the eighth protrusion structure and the sidewall of the eighth protrusion structure near the seventh protrusion structure form a ninth recess structure with the bottom of the first substrate; The ninth protrusion structure and the tenth protrusion structure are respectively disposed on both sides of the top of the second substrate. The side wall of the ninth protrusion structure near the tenth protrusion structure and the side wall of the tenth protrusion structure near the ninth protrusion structure form a tenth recessed structure with the top of the second substrate. The fourth liquid inlet hole, the fourth liquid outlet hole, the second limiting groove and the third limiting groove are all disposed at the bottom of the tenth recessed structure. When the nucleic acid synthesis module is assembled, the ninth recessed structure and the tenth recessed structure combine to form the receiving cavity, the seventh recessed structure and the ninth protruding structure combine to form the fifth male and female structure, and the eighth recessed structure and the tenth protruding structure combine to form the sixth male and female structure.

8. The nucleic acid synthesis module according to claim 3 or 6, characterized in that, The nucleic acid synthesis module also includes multiple fixing components. The upper cover has multiple first mounting holes, and the lower cover has multiple second mounting holes. The fixing components pass vertically through the first mounting holes and the second mounting holes to fix the upper cover and the lower cover together.

9. The nucleic acid synthesis module according to claim 1, characterized in that, The synthesized chip also includes a wiring area and a flexible printed circuit. Both the wiring area and the flexible printed circuit are located outside the receiving cavity. The synthesis reaction area is connected to one end of the flexible printed circuit through the wiring area, and the other end of the flexible printed circuit is used to connect to the driving system.

10. A nucleic acid synthesis system, characterized in that, The nucleic acid synthesis system includes the nucleic acid synthesis module as described in any one of claims 1-9.

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