Column chromatography fully automatic liquid collector

Abstract

The utility model discloses a column chromatography full -automatic liquid collector belongs to column chromatography device technical field, including bottom plate, host computer, the host computer includes microcomputer, touch display screen and control switch, microcomputer and touch display screen and control switch are all electricity connection, rotating test tube bracket, rotating test tube bracket rotatablely connected on the bottom plate, a plurality of test tube holes are seted up on rotating test tube bracket top, a plurality of test tube holes are distributed as three groups of concentric circles with the center of rotating test tube bracket's circle as the center, and the test tube is placed in the test tube hole. The utility model discloses through the accurate control of microcomputer in host computer to first drive assembly and second rotating motor, has realized the automatic collaborative work of rotating test tube bracket and sample head frame, does not need manual frequent replacement test tube and adjustment drop position, has reduced the labor intensity of staff greatly, satisfies the experiment demand of convenient and fast, has improved the collection efficiency.

Description

Technical Field

[0001] This utility model belongs to the technical field of column chromatography devices, and particularly relates to a fully automatic liquid collector for column chromatography. Background Technology

[0002] Column chromatography is widely used in biology, medicine, and chemical engineering. Its principle is based on the different affinities of compounds between the stationary and mobile phases, enabling the separation of compounds for purification, separation of mixture components, and analysis of compound concentrations. During column chromatography experiments, the eluent needs to be collected. Traditional collection methods are mostly manual, requiring frequent manual replacement of the collection container once it is full. This is cumbersome, labor-intensive, and prone to errors, leading to sample loss and contamination of the workbench. Furthermore, manual operation makes it difficult to guarantee the accuracy and consistency of collection, potentially affecting the reliability of experimental results.

[0003] In existing technologies, complex robotic arms are used for liquid collection operations. However, this method has a high failure rate and maintenance costs during use, which cannot meet the needs of convenient and rapid experiments and can easily affect the efficiency of liquid collection. Utility Model Content

[0004] The purpose of this invention is to propose a fully automated liquid collector for column chromatography, in order to solve the problems of high failure rate and maintenance cost of robotic arms in traditional technologies, which cannot meet the needs of convenient and rapid experiments and easily affect the liquid collection efficiency.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] The fully automated column chromatography liquid collector includes:

[0007] Base plate;

[0008] The host includes a microcomputer, a touch screen, and a control switch, wherein the microcomputer is electrically connected to the touch screen and the control switch.

[0009] A rotating test tube holder is rotatably connected to a base plate. The top of the rotating test tube holder has multiple test tube holes, which are arranged in three concentric circles with the center of the rotating test tube holder as the center. Test tubes are placed in the test tube holes.

[0010] A sample dispensing head is rotatably connected to a base plate. When the sample dispensing head rotates, the end of the head away from the base plate covers part of the test tube holes on three sets of concentric circles.

[0011] Preferably, the base plate has a rotating groove, the rotating test tube holder is rotatably connected inside the rotating groove, the base plate has an installation groove, the installation groove is connected to a first driving component, the output end of the first driving component is connected to the rotating test tube holder, and is used to drive the rotating test tube holder to rotate, and the first driving component is electrically connected to a microcomputer.

[0012] Preferably, the first drive assembly includes a first motor base fixedly connected inside the mounting slot, a first rotary motor mounted on the first motor base, a rotating shaft coaxially fixedly connected to the output end of the first rotary motor, a drive gear coaxially fixedly connected to the end of the rotating shaft away from the first rotary motor, an external gear ring coaxially fixedly connected to the outer side of the rotating test tube holder, the drive gear meshing with the external gear ring, and a receiving cavity provided inside the base plate for the drive gear and the external gear ring to rotate. The first rotary motor is a stepper motor and is electrically connected to a microcomputer.

[0013] Preferably, the rotating test tube holder includes a base rotatably connected inside the mounting groove and a bracket fixedly connected to the base. The external gear ring is coaxially fixedly connected to the outside of the base. Multiple test tube holes are opened on the bracket. The number of multiple test tube holes gradually increases from the inside to the outside on three sets of concentric circles, and the multiple test tube holes on the same concentric circle are evenly distributed along the circumferential direction.

[0014] Preferably, a second motor base is fixedly connected to the base plate, a second rotary motor is mounted on the second motor base, and the bottom of the sample feeding head frame is coaxially fixedly connected to the output end of the second rotary motor. The second rotary motor is a stepper motor and is electrically connected to a microcomputer.

[0015] Preferably, the sample dispensing head frame includes a vertical rod detachably connected to the output end of a second rotary motor, a connecting frame slidably connected to the top of the vertical rod, a horizontal rod slidably connected inside the connecting frame, and screws threadedly connected to both sides of the connecting frame. The ends of the two screws abut against the vertical rod and the horizontal rod respectively, and the end of the horizontal rod away from the vertical rod is connected to a drip tube with a clamping assembly.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. This utility model achieves automatic coordinated operation of the rotating test tube holder and the sample dispensing head frame through precise control of the first drive component and the second rotating motor by the microcomputer in the host. It completely covers the test tubes on multiple concentric circles and performs fully automatic liquid dispensing operation. It eliminates the need for frequent manual replacement of test tubes and adjustment of the liquid dispensing position, greatly reduces the labor intensity of the staff, meets the needs of convenient and fast experiments, and improves the liquid collection efficiency.

[0018] 2. In this utility model, the test tube holes on the rotating test tube holder are designed with three sets of concentric circles distributed in an increasing number from the inside to the outside, which can adapt to the placement needs of test tubes of different quantities and specifications. At the same time, the adjustable structure of the sample feeding head can adapt to test tubes of different heights, meeting diverse experimental requirements. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of the fully automated column chromatography liquid collector proposed in this utility model;

[0020] Figure 2 This is a schematic diagram of the chassis structure of the fully automated column chromatography liquid collector proposed in this utility model;

[0021] Figure 3 This is a partial vertical sectional view of the bottom of the fully automated column chromatography liquid collector proposed in this utility model;

[0022] Figure 4 for Figure 3 Enlarged diagram of part A in the middle.

[0023] In the diagram: 1. Base plate; 2. Main unit; 3. Touch screen; 4. Control switch; 5. Mounting slot; 6. First motor base; 7. First rotary motor; 8. Rotating shaft; 9. Drive gear; 10. Rotating slot; 11. Rotating test tube holder; 12. External gear ring; 13. Test tube hole; 14. Second motor base; 15. Second rotary motor; 16. Sample feeding head holder. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0025] Reference Figures 1-4 The fully automated column chromatography liquid collector includes:

[0026] Base plate 1.

[0027] Host 2 includes a microcomputer, a touch screen 3, and a control switch 4. The microcomputer is electrically connected to the touch screen 3 and the control switch 4.

[0028] Install the host 2 in a suitable position on the base plate 1 and fix it with bolts or other connectors. Connect the wires between the microcomputer inside the host 2 and the touch screen 3 and control switch 4 to ensure that the circuit connection is correct and secure.

[0029] A rotating test tube holder 11 is rotatably connected to the base plate 1. Multiple test tube holes 13 are provided on the top of the rotating test tube holder 11. The multiple test tube holes 13 are distributed in three concentric circles with the center of the rotating test tube holder 11 as the center. Test tubes are placed in the test tube holes 13.

[0030] The rotating test tube holder 11 includes a base rotatably connected inside the mounting groove 5 and a bracket fixedly connected to the base. An external gear ring 12 is coaxially fixedly connected to the outside of the base. Multiple test tube holes 13 are opened on the bracket. The number of multiple test tube holes 13 gradually increases from the inside to the outside on three sets of concentric circles, and the multiple test tube holes 13 on the same concentric circle are evenly distributed along the circumference.

[0031] The chassis has multiple limiting holes, which correspond one-to-one with the test tube holes 13, thus limiting the bottom of the test tube and improving the stability of the test tube during movement.

[0032] A rotating groove 10 is provided on the base plate 1, and a rotating test tube holder 11 is rotatably connected inside the rotating groove 10. An installation groove 5 is provided on the base plate 1, and a first driving component is connected inside the installation groove 5. The output end of the first driving component is connected to the rotating test tube holder 11 and is used to drive the rotating test tube holder 11 to rotate. The first driving component is electrically connected to a microcomputer.

[0033] The first drive assembly includes a first motor base 6 fixedly connected inside the mounting slot 5, a first rotary motor 7 mounted on the first motor base 6, a rotary shaft 8 coaxially fixedly connected to the output end of the first rotary motor 7, a drive gear 9 coaxially fixedly connected to the end of the rotary shaft 8 away from the first rotary motor 7, an external gear ring 12 coaxially fixedly connected to the outside of the rotating test tube holder 11, the drive gear 9 and the external gear ring 12 meshing with each other, and a receiving cavity for the drive gear 9 and the external gear ring 12 to rotate is opened inside the base plate 1. The first rotary motor 7 is a stepper motor and is electrically connected to a microcomputer.

[0034] The sample feeding head 16 is rotatably connected to the base plate 1. When the sample feeding head 16 rotates, the end of it away from the base plate 1 covers part of the test tube holes 13 on the three sets of concentric circles.

[0035] A second motor mount 14 is fixedly connected to the base plate 1. A second rotary motor 15 is mounted on the second motor mount 14. The bottom of the sample feeding head frame 16 is coaxially fixedly connected to the output end of the second rotary motor 15. The second rotary motor 15 is a stepper motor and is electrically connected to a microcomputer.

[0036] The first rotary motor 7 and the second rotary motor 15 are controlled by a microcomputer. The output of the first rotary motor 7 drives the rotating test tube holder 11 to rotate through the rotating shaft 8, the drive gear 9 and the external gear ring 12, so that the test tubes in all the test tube holes 13 can move to the bottom of the dropper fixed in the sample dispensing head frame 16. The second rotary motor 15 drives the sample dispensing head frame 16 to rotate, so that the fixed dropper can switch on different concentric circles, and finally complete the dispensing of all the test tubes.

[0037] The sample feeding head holder 16 includes a vertical rod detachably connected to the output end of the second rotary motor 15. A connecting frame is slidably connected to the top of the vertical rod, and a horizontal rod is slidably connected inside the connecting frame. Both sides of the connecting frame are threaded with screws. The ends of the two screws abut against the vertical rod and the horizontal rod, respectively. The end of the horizontal rod away from the vertical rod is connected to a drip tube with a clamping assembly.

[0038] By rotating the screw, staff can easily adjust the initial height of the connecting frame and the initial length of the crossbar, facilitating the initial calibration of the dripping.

[0039] When this utility model is in operation, the test tubes containing the eluent to be collected are placed sequentially into the test tube holes 13 of the rotating test tube holder 11, and the appropriate test tube placement position and number are selected according to the requirements.

[0040] Secure the dropper to the sample head holder 16 using the external clamping assembly, turn on the main unit 2, and set relevant parameters via the touch screen 3, such as the collection time interval, the rotation sequence and angle of the rotating test tube holder 11.

[0041] Press the start button in the control switch 4. The microcomputer in the host 2 controls the second rotary motor 15 to drive the sample dispensing head frame 16 to rotate according to the set parameters, so that the dropper moves directly above the first test tube to be collected. At the same time, the microcomputer controls the first rotary motor 7 to put the rotating test tube holder 11 in the initial position.

[0042] Once the dropper is aligned with the test tube, the eluent is added. During the addition process, the microcomputer monitors the time in real time. When the set collection time interval is reached, the microcomputer controls the first rotary motor 7 to start, driving the rotating test tube holder 11 to rotate at a certain angle, so that the next test tube moves directly below the dropper, completing the collection of eluent from all test tubes on the same concentric circle. Then, the microcomputer controls the second rotary motor 15 to drive the sample dispensing head holder 16 to rotate, so that the dropper moves directly above a test tube on the next concentric circle, and the collection of eluent from all test tubes on this concentric circle is repeated until the collection of eluent from all the set test tubes is completed.

[0043] Alternatively, it can be used in conjunction with external detection equipment such as an ultraviolet detector. The microcomputer is electrically connected to the external detection equipment, and by acquiring the detection signal from the external detection equipment, the first rotating motor 7 is controlled to further achieve fully automatic separation.

[0044] After collection is completed, the microcomputer controls the first rotary motor 7 and the second rotary motor 15 to stop working, the sample feeding head frame 16 and the rotating test tube holder 11 return to their initial positions, the power of the main unit 2 is turned off, the test tubes containing the collected eluent are removed for subsequent experimental analysis, and the device is cleaned to prepare for the next experiment.

[0045] After each experiment, promptly clean the residual liquid and impurities on the rotating test tube holder 11 to prevent them from corroding the rotating test tube holder 11.

[0046] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A fully automated column chromatography liquid collector, characterized in that, include: Base plate (1); The host (2) includes a microcomputer, a touch screen (3) and a control switch (4), and the microcomputer is electrically connected to the touch screen (3) and the control switch (4); A rotating test tube holder (11) is rotatably connected to a base plate (1). The top of the rotating test tube holder (11) has multiple test tube holes (13). The multiple test tube holes (13) are arranged in three concentric circles with the center of the rotating test tube holder (11) as the center. Test tubes are placed in the test tube holes (13). The sample dispensing head (16) is rotatably connected to the base plate (1). When the sample dispensing head (16) rotates, the end of it away from the base plate (1) covers part of the test tube holes (13) on three sets of concentric circles.

2. The fully automated column chromatography liquid collector according to claim 1, characterized in that, The base plate (1) has a rotating groove (10), and the rotating test tube holder (11) is rotatably connected inside the rotating groove (10). The base plate (1) has an installation groove (5), and a first driving component is connected inside the installation groove (5). The output end of the first driving component is connected to the rotating test tube holder (11) to drive the rotating test tube holder (11) to rotate. The first driving component is electrically connected to a microcomputer.

3. The fully automated column chromatography liquid collector according to claim 2, characterized in that, The first drive assembly includes a first motor base (6) fixedly connected inside the mounting slot (5), a first rotary motor (7) is mounted on the first motor base (6), a rotary shaft (8) is coaxially fixedly connected to the output end of the first rotary motor (7), a drive gear (9) is coaxially fixedly connected to the end of the rotary shaft (8) away from the first rotary motor (7), an external gear ring (12) is coaxially fixedly connected to the outside of the rotating test tube holder (11), the drive gear (9) and the external gear ring (12) mesh with each other, and a receiving cavity is opened inside the base plate (1) for the drive gear (9) and the external gear ring (12) to rotate. The first rotary motor (7) is a stepper motor and is electrically connected to a microcomputer.

4. The fully automated column chromatography liquid collector according to claim 3, characterized in that, The rotating test tube holder (11) includes a base rotatably connected inside the mounting groove (5) and a bracket fixedly connected to the base. The external gear ring (12) is coaxially fixedly connected to the outside of the base. Multiple test tube holes (13) are opened on the bracket. The number of multiple test tube holes (13) gradually increases from the inside to the outside on three sets of concentric circles, and the multiple test tube holes (13) on the same concentric circle are evenly distributed along the circumferential direction.

5. The fully automated column chromatography liquid collector according to claim 1, characterized in that, A second motor mount (14) is fixedly connected to the base plate (1), and a second rotary motor (15) is installed on the second motor mount (14). The bottom of the sample feeding head frame (16) is coaxially fixedly connected to the output end of the second rotary motor (15). The second rotary motor (15) is a stepper motor and is electrically connected to a microcomputer.

6. The fully automated column chromatography liquid collector according to claim 5, characterized in that, The sample dispensing head frame (16) includes a vertical rod detachably connected to the output end of a second rotary motor (15). A connecting frame is slidably connected to the top of the vertical rod, and a horizontal rod is slidably connected inside the connecting frame. Both sides of the connecting frame are threaded with screws, and the ends of the two screws abut against the vertical rod and the horizontal rod respectively. The end of the horizontal rod away from the vertical rod is connected to a drip tube with a clamping assembly.

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