A microalgae cultivation device integrated with a container

Abstract

The utility model relates to a kind of microalgae culture devices of container integrated type, comprising: transparent continuous curved U-shaped pipeline, with water inlet and water outlet;Support, the U-shaped pipeline is fixed on the outer surface of container;Dark reaction box, be located in container interior and be connected by pipeline pneumatic diaphragm pump, the water inlet is connected dark reaction box by pneumatic diaphragm pump, the water outlet directly communicates dark reaction box, constitutes closed circulation system.The microalgae culture device is a kind of modularization equipment with high integration, low operating cost and can efficiently produce, can realize the synergistic effect of wastewater treatment and resource utilization.

Description

Technical Field

[0001] This utility model relates to the field of aquaculture wastewater treatment and microalgae cultivation technology, and in particular to a containerized microalgae cultivation device that integrates wastewater treatment and microalgae resource utilization. Background Technology

[0002] With the intensive development of aquaculture, wastewater treatment has become a key issue restricting the industry's sustainable development. Traditional treatment processes (such as SBR activated sludge process and biological filters) generally have high construction costs (approximately 800-1200 yuan per ton of water treatment facility) and large land area requirements (average daily treatment capacity of 100m³). 3 Issues such as requiring 150-200㎡ of space were encountered, and the nitrogen and phosphorus resources in the wastewater were not effectively recycled.

[0003] Against this backdrop, there is an urgent need to develop a modular equipment with high integration, low operating costs, and high production efficiency to achieve synergistic effects between wastewater treatment and resource utilization. Utility Model Content

[0004] This utility model aims to at least partially solve one of the technical problems existing in the prior art. To this end, this utility model provides a container-integrated microalgae cultivation device, including: a transparent, continuous, curved U-shaped pipe with an inlet and an outlet; a support frame that fixes the U-shaped pipe to the outer surface of a container; and a dark reaction chamber located inside the container and connected to a pneumatic diaphragm pump via a pipe. The inlet is connected to the dark reaction chamber via the pneumatic diaphragm pump, and the outlet is directly connected to the dark reaction chamber, forming a closed-loop system.

[0005] Preferably, the bracket fixes the U-shaped pipe to the outer surface of the container in a multi-layered stepped arrangement, forming a three-dimensional flow channel that starts from the first layer on the side of the container, extends through the first layer on the top surface to the second layer on the top surface, and finally returns to the second layer on the side of the container.

[0006] Preferably, the multi-layered stepped arrangement of the U-shaped pipes achieves flow channel connection through three-segment spatial turns. The U-shaped pipe at the end of the first layer on the side of the container is horizontally coplanar adjacent to the U-shaped pipe at the beginning of the first layer on the top surface. The U-shaped pipe at the end of the first layer on the top surface is vertically coplanar adjacent to the U-shaped pipe at the beginning of the second layer on the top surface. The U-shaped pipe at the end of the second layer on the top surface is vertically coplanar adjacent to the U-shaped pipe at the beginning of the second layer on the side of the container.

[0007] Preferably, the support includes two rows of side supports and two rows of top supports arranged in parallel, wherein: the lateral spacing of the two rows of side supports is equal to the center distance between the pipes of the first and second layers of the container side; the vertical spacing of the two rows of top supports is equal to the center distance between the pipes of the first and second layers of the top surface; and the U-shaped pipe is detachably fixed to the preset anchor point of the support by clamps.

[0008] Preferably, the container also includes a pretreatment unit located at the front of the container. The pretreatment unit includes a funnel-shaped bottom water collection tank. The outlet of the water collection tank is connected to the wastewater inlet of the dark reaction tank through a diversion pipe. The diversion pipe is equipped with an adjustable valve to control the wastewater input flow rate.

[0009] Preferably, it also includes a microalgae propagation device located inside the container, comprising at least two sets of vertically arranged transparent acrylic column reactors; the outlet of the microalgae propagation device is connected to the algae seed input port of the dark reaction chamber via a diversion valve, the diversion valve being able to switch between connected states to control the timing of algae seed delivery.

[0010] Preferably, it also includes a collection device located at the end of the container, which is connected to the bottom algae discharge port of the dark reaction tank via a pneumatic diaphragm pump; an electromagnetic valve and a concentration sensor are connected in series on the inlet pipe of the collection device, wherein: the concentration sensor monitors the algal biomass concentration in real time, and the electromagnetic valve opens the collection channel in response to the concentration signal.

[0011] Preferably, the dark reaction chamber is equipped with a temperature coordination control system. When the temperature sensor detects a value greater than 28°C, the air conditioner is activated to maintain the temperature inside the chamber within a gradient range of 18-30°C.

[0012] Preferably, the dark reaction chamber is equipped with a gas-liquid mixing device, including an annular gas distribution pipe and a microporous aerator connected thereto. The aerator has a pore diameter of 0.1-0.2 mm and a surface hydrophobic angle >150°.

[0013] This patented containerized integrated microalgae cultivation device achieves a significant leap in efficiency for industrial-scale microalgae cultivation through multi-level technological innovation. In terms of space utilization, it employs a unique three-dimensional flow channel design, fixing transparent U-shaped pipes to the outer surface of the container. A continuous flow channel is formed through three-segment spatial transitions (horizontal and vertical sections are coplanar and adjacent), maximizing the use of the container's side and top space. Compared to traditional planar cultivation systems, this design increases the light-receiving area by over 200% and avoids pipe cross-blocking, ensuring uniform light distribution. Simultaneously, all functional units (dark reaction chamber, expansion culture) are highly integrated within a standard container, forming a modular "plug-and-play" system, significantly reducing site requirements and infrastructure costs, making it particularly suitable for space-constrained scenarios such as ports and factories.

[0014] In terms of biomass production efficiency, the device constructs a closed-loop system that synergistically enhances both light and dark reactions. An externally mounted, continuously curved U-shaped pipe serves as the light reaction zone; its unique structure enhances turbulence, reducing algal cell adhesion and death. Combined with direct natural light, this increases photosynthetic efficiency by 30%-40%. The dark reaction chamber integrates microporous aerators (0.1-0.2 mm pore size, hydrophobic angle >150°) to provide ultra-microbubble oxygen, promoting the dark reaction process and simultaneously resolving growth inhibition caused by excessive light. Combined with temperature sensors and air conditioning for temperature control, this dual-effect synergy drives increased biomass accumulation rates, resolving growth inhibition caused by temperature fluctuations.

[0015] In terms of resource recycling and energy consumption control, the device achieves triple optimization of water, energy, and the harvesting process. The funnel-shaped collection tank in the pretreatment unit recovers wastewater, which is then diverted to the dark reaction tank via adjustable valves. Combined with a U-shaped pipeline-dark reaction tank closed loop, this achieves a water saving rate of 90%. The intelligent harvesting system monitors algal biomass in real time using concentration sensors and controls the start and stop of the pneumatic diaphragm pump via a solenoid valve, triggering collection only when standards are met, thus reducing ineffective energy consumption. The low-shear characteristics of the pneumatic diaphragm pump simultaneously ensure algal cell activity, avoiding yield loss due to mechanical damage.

[0016] In terms of operation, maintenance, and economy, the modular design significantly improves system reliability. The propagation unit shortens the algae cultivation cycle through vertically parallel acrylic column reactors, and the diversion valve precisely controls the timing of algae input, achieving seamless integration of propagation and cultivation. The support clamp anchor point design supports quick disassembly and assembly of U-shaped pipes, allowing for individual section replacement in case of localized damage. Utilizing recycled shipping containers reduces equipment costs, while the synergistic effect of natural light dominance, wastewater reuse, and intelligent temperature control further reduces operating costs. The three-dimensional flow channel design enables algae production per unit volume to reach several times that of traditional systems, providing a rapidly deployable industrial solution for the energy and food applications of microalgae. Attached Figure Description

[0017] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0018] Figure 1 This is a first schematic diagram of a containerized integrated microalgae cultivation device provided according to an embodiment of the present invention.

[0019] Figure 2 This is a second schematic diagram of the container-integrated microalgae cultivation device provided according to an embodiment of the present invention.

[0020] Figure 3 This is a third schematic diagram of the container-integrated microalgae cultivation device provided according to an embodiment of the present utility model.

[0021] Figure 4 This is a fourth schematic diagram of the container-integrated microalgae cultivation device provided according to an embodiment of the present invention. Detailed Implementation

[0022] The following embodiments are provided to enable those skilled in the art to better understand the present invention. It should be noted that the following embodiments do not constitute a limitation on the scope of protection claimed by the present invention, but are merely illustrative examples. Unless otherwise specified, the raw materials, reagents, or devices mentioned in the following embodiments are commercially available or obtained through known existing methods.

[0023] Example 1: Core Structure and Three-Dimensional Flow Channel Design

[0024] This embodiment describes in detail the core structure and three-dimensional flow channel design of the microalgae cultivation device.

[0025] This embodiment provides a container-integrated microalgae cultivation device, such as... Figure 1 As shown, the system includes: a transparent, continuously curved U-shaped pipe with an inlet and an outlet; a support frame to fix the U-shaped pipe to the outer surface of the container; and a dark reaction chamber located inside the container and connected to a pneumatic diaphragm pump via a pipe. The inlet is connected to the dark reaction chamber via the pneumatic diaphragm pump, and the outlet is directly connected to the dark reaction chamber, forming a closed-loop system. The support frame fixes the U-shaped pipe to the outer surface of the container in a multi-layered, stepped arrangement, forming a three-dimensional flow channel that starts from the first layer on the side of the container, extends through the first layer on the top surface to the second layer on the top surface, and finally returns to the second layer on the side of the container. The multi-layered, stepped arrangement of the U-shaped pipe achieves flow channel connection through three-segment spatial transitions. The U-shaped pipe at the end of the first layer on the side of the container is basically horizontally and coplanarly adjacent to the U-shaped pipe starting from the first layer on the top surface; the U-shaped pipe at the end of the first layer on the top surface is basically perpendicularly and coplanarly adjacent to the U-shaped pipe starting from the second layer on the top surface; and the U-shaped pipe at the end of the second layer on the top surface is basically perpendicularly and coplanarly adjacent to the U-shaped pipe starting from the second layer on the side of the container (meaning that angular deviation is permissible). The support system comprises two rows of parallel side supports and two rows of top supports, wherein: the lateral spacing of the two rows of side supports is equal to the center distance between the pipes of the first and second layers of the container side; the vertical spacing of the two rows of top supports is equal to the center distance between the pipes of the first and second layers of the top surface; and the U-shaped pipes are detachably fixed to the preset anchor points of the support system by clamps.

[0026] In this embodiment, the core photoreactor is a transparent, continuously bent U-shaped pipe (an externally mounted photoreactor). This pipe is typically made of high-transmittance (>90%) glass tubing, with an inner diameter of approximately 50-100 mm and a wall thickness of approximately 3-5 mm, ensuring excellent light transmission performance. One end of the pipe is an inlet, and the other end is an outlet.

[0027] like Figure 2 As shown, the brackets are used to securely and detachably fix the U-shaped pipes to the outer surface of a standard shipping container (e.g., 20-foot or 40-foot). The brackets are designed as follows: two rows of side brackets are installed parallel to each other on the same side of the container (e.g., the left side or the sun-facing side), with their lateral spacing approximately equal to the center-to-center distance between the pipes of the first and second layers of the container's side surface as defined above (e.g., a spacing of 300mm). Two rows of top brackets are installed parallel to each other on the top surface of the container, with their vertical spacing approximately equal to the center-to-center distance between the pipes of the first and second layers of the top surface as defined above (e.g., a spacing of 250mm). The U-shaped pipes are detachably fixed to pre-set anchor points on the brackets using clamps (e.g., stainless steel U-bolts or special plastic clips). These anchor points are arranged strictly according to multi-layered, stepped layout requirements.

[0028] The entire U-shaped pipe array is arranged in three spatial turns to form a continuous three-dimensional flow channel. Initial section (first layer on the side of the container): The U-shaped pipe is horizontally fixed to the upper anchor point of the side support. First turn and extension section (first layer on the top): The U-shaped pipe at the end of the first layer on the side of the container and the U-shaped pipe at the beginning of the first layer on the top are horizontally coplanar adjacent at the top edge of the container side (i.e., the two pipe sections are seamlessly connected on the same horizontal plane or smoothly connected through standard fittings). After turning 90 degrees, the pipe is laid horizontally on the upper anchor point of the top support. Second turn and extension section (second layer on the top): The U-shaped pipe at the end of the first layer on the top and the U-shaped pipe at the beginning of the second layer on the top are vertically coplanar adjacent on the top surface (i.e., the two pipe sections are seamlessly connected on the same vertical plane or smoothly connected through standard fittings, usually turning downwards by 90 degrees). After turning, the pipe is laid horizontally on the lower anchor point of the top support. The third turning and termination section (second layer on the side of the container): The U-shaped pipe at the end of the second layer on the top surface and the U-shaped pipe at the beginning of the second layer on the side of the container are vertically coplanarly connected at the top edge of the container on the other side (i.e., the two pipe sections are seamlessly connected on the same vertical plane or smoothly connected through standard pipe fittings, usually turning downwards at 90 degrees). After turning, the pipe is laid vertically on the lower anchor point of the side support. This design forms a complete and continuous three-dimensional lighting channel starting from the first layer on the side of the container → extending through the first layer on the top surface → turning to the second layer on the top surface → and finally returning to the second layer on the side of the container to terminate, maximizing the use of the lighting area of ​​the container's outer surface.

[0029] The dark reaction chamber is installed inside the container. The outlet of the U-shaped pipe is directly connected to the inlet at the top of the dark reaction chamber via a pipe. The inlet of the U-shaped pipe is then connected to the outlet of the pneumatic diaphragm pump via a pipe. The inlet of the pneumatic diaphragm pump is connected to the outlet at the bottom of the dark reaction chamber via a pipe. Thus, the algal solution forms a closed loop driven by the pneumatic diaphragm pump: dark reaction chamber → pump → U-shaped pipe inlet → U-shaped pipe (for photosynthesis) → U-shaped pipe outlet → dark reaction chamber.

[0030] Example 2: Pretreatment, Propagation and Intelligent Collection System

[0031] like Figure 3 As shown in the figure, this embodiment describes in detail the pretreatment unit, the microalgae propagation device, and the collection device.

[0032] The microalgae cultivation device also includes a pretreatment unit located at the front of the container, comprising a funnel-shaped bottom collection tank. The outlet of the collection tank is connected to the wastewater inlet of the dark reaction tank via a diversion pipe, which is equipped with an adjustable valve to control the wastewater input flow rate. It also includes a microalgae propagation device located inside the container, comprising at least two sets of vertically parallel transparent acrylic column reactors. The outlet of the microalgae propagation device is connected to the algae seed inlet of the dark reaction tank via a diversion valve, which can switch between connected and disconnected states to control the timing of algae seed delivery. Finally, it includes a collection device located at the rear of the container, connected to the bottom algae discharge port of the dark reaction tank via a pneumatic diaphragm pump. An electromagnetic valve and a concentration sensor are connected in series on the inlet pipe of the collection device. The concentration sensor monitors the algal biomass concentration in real time, and the electromagnetic valve opens the collection channel in response to the concentration signal.

[0033] A pretreatment unit is installed on the exterior of the container. Its core is a funnel-shaped collection tank located at the bottom of the unit. The bottom of this collection tank is designed with a sufficient inclination angle (e.g., 60°-75°) to form a funnel shape, facilitating the collection and discharge of sediments in the wastewater and preventing the accumulation of particulate matter. The outlet of the collection tank connects to a diversion pipe. Adjustable valves (such as manual ball valves or electric regulating valves) are installed on the diversion pipe to precisely control the flow rate of wastewater entering the dark reaction tank. The other end of the diversion pipe connects to a dedicated wastewater inlet on the dark reaction tank. The pre-treated wastewater can be quantitatively introduced into the cultivation system as needed.

[0034] Inside the container is a microalgae propagation unit. This unit comprises at least two sets of vertically arranged transparent acrylic column reactors (e.g., cylinders with a diameter of 200 mm and a height of 1.5 m). Each column reactor is equipped with independent lighting (e.g., LED light source) and aeration systems for the pre-cultivation (expansion) of high-density algae. The common outlet of the microalgae propagation unit (or the combined outlets of each reactor set) is connected via piping to a diversion valve (e.g., a three-way solenoid valve or an electric ball valve). The outlet of the diversion valve is connected to a dedicated algae inlet on the dark reaction chamber. The core function of the diversion valve is to switch between connected and disconnected states. When algae replenishment is needed, the valve switches to the "connected" state, inputting the pre-propagated high-concentration algae solution into the dark reaction chamber. When replenishment is not needed, the valve switches to the "closed" or "bypass" state, controlling the timing of algae delivery.

[0035] An external collection device (such as a storage tank or centrifuge feed tank) is installed on the outside of the container. The inlet of the collection device is connected via a pipe to the outlet of a pneumatic diaphragm pump (which can be a circulation pump, but more commonly a dedicated collection pump). The inlet of this pneumatic diaphragm pump is then connected via a pipe to the algae discharge port at the bottom of the dark reaction tank. A solenoid valve (such as a normally closed two-position two-way solenoid valve) is installed in series on the inlet pipe connecting to the collection device to control the opening and closing of the collection pipe. A concentration sensor (such as an optical density (OD) sensor or a biomass probe) monitors the biomass concentration of the algal solution discharged from the dark reaction tank in real time.

[0036] The concentration sensor transmits the real-time concentration signal (such as g / L or OD value) to the central controller (PLC or microprocessor). The controller has a preset target collection concentration threshold (e.g., biomass concentration > 1.5 g / L). When the concentration sensor value reaches or exceeds the preset threshold, the controller issues a command to open the solenoid valve and simultaneously start the collection pump (or switch the pump's flow direction) to pump the high-concentration algal solution into the collection device for harvesting. When the concentration falls below the threshold, the solenoid valve closes, stopping collection. This ensures efficient harvesting only when the algal solution reaches the ideal concentration.

[0037] Example 3: Optimization of Dark Reaction Chamber Environmental Control

[0038] This embodiment describes in detail the key systems inside the dark reaction chamber.

[0039] The dark reaction chamber is equipped with a temperature coordination control system, including: a spiral heat exchange coil embedded in the side wall of the dark reaction chamber and connected to the air conditioning refrigeration circuit inside the container; and distributed temperature sensors arranged in the upper, middle, and lower liquid level layers of the dark reaction chamber. When the temperature sensor reading at any layer exceeds 28°C, the air conditioning system activates, driving the coolant to flow through the coil, maintaining the temperature inside the chamber within a gradient range of 18-30°C. The dark reaction chamber also includes a gas-liquid mixing device, comprising an annular gas distribution pipe and connected microporous aerators. The aerators have pore sizes of 0.1-0.2 mm and a surface hydrophobic angle greater than 150°.

[0040] Inside the dark reaction chamber, a sophisticated temperature control system is integrated. Rotating heat exchange coils are tightly embedded in the side walls (or bottom / interior) of the chamber, and the coils are typically made of stainless steel or corrosion-resistant alloys. The inlet and outlet of the coils are connected to the refrigeration circuit of the air conditioning system installed inside the container via pipes and valves. Distributed temperature sensors are installed at different liquid level layers inside the dark reaction chamber, specifically at three representative liquid level layers (e.g., 10cm below the liquid surface, in the middle of the chamber, and 10cm near the bottom) to comprehensively monitor the temperature distribution within the chamber.

[0041] The central controller continuously receives readings from three temperature sensors. When any sensor reading exceeds 28°C, the controller activates the air conditioning cooling mode. Refrigerant (or coolant) is driven to flow through heat exchange coils embedded in the chamber walls, absorbing heat from the algal solution within the dark reaction chamber. By adjusting the cooling power or coolant flow rate, the system strictly maintains the overall temperature within the dark reaction chamber within the target range of 18-30°C, with a control accuracy of ±1°C (e.g., when set to 25°C, the actual temperature is maintained at 24-26°C).

[0042] At the bottom of the dark reaction chamber, a high-efficiency gas-liquid mixing device is installed. The core of this device includes a ring-shaped air distribution pipe, located at the bottom of the chamber. Multiple interfaces are evenly distributed on the ring pipe. Each interface connects to a microporous aerator head. These aerator heads have the following key characteristics: pore size range of 0.1-0.2 mm. This microporous design generates fine bubbles, increasing the gas-liquid contact area and dissolved oxygen (DO) mass transfer efficiency. Surface hydrophobic angle >150°. Manufactured using highly hydrophobic materials (such as specially treated PTFE or silica gel) to ensure smooth bubble release from the micropores, preventing pore blockage by water or algal liquid and maintaining long-term stable aeration performance. The ring-shaped air distribution pipe is connected to an external gas source (such as an air compressor or CO2 mixed gas source) via a pipe. Gas (air, CO2, or a mixture) is released through the microporous aerator heads, forming a large number of fine bubbles, achieving efficient gas-liquid mixing and gas dissolution within the dark reaction chamber, meeting the gas requirements of the microalgae's dark reaction stage (respiration, carbon assimilation).

[0043] Example 4: Containerized Integration and System Operation

[0044] This embodiment comprehensively describes the layout, connection, and operation process of the entire container integration system.

[0045] The entire microalgae cultivation system is highly integrated within and around a standard shipping container. Externally, U-shaped pipes, secured to the container's sides and top surface via precisely positioned supports and clamps, are arranged in a three-section spatial transition and multi-layered stepped configuration, forming a highly efficient light reaction zone. The entire space is rationally divided into different functional areas: the front section houses the pretreatment unit (including a funnel-shaped water collection tank); the middle section contains the core dark reaction chamber (with built-in temperature control coils, gas-liquid mixing device, and distributed temperature sensors), and the microalgae propagation device (vertically arranged parallel acrylic columns, such as…). Figure 4 (As shown). A collection device is placed at the rear / end. Necessary pneumatic diaphragm pumps (and possibly a collection pump), valves (adjustable valves, diverter valves, solenoid valves), sensors (concentration sensors), control system cabinets, air conditioning units, etc., are also installed in appropriate locations within the enclosure. All functional units (U-tube, dark reaction chamber, pretreatment, expansion culture, collection, pumps, valves, sensors, air conditioning) are precisely connected according to the preset process and control logic via corrosion-resistant pipes (such as PVC, PPR, or stainless steel pipes), cables, and signal lines.

[0046] The pipe connection ensures the aforementioned closed loop. It also includes key logistics paths such as wastewater input (pretreatment → dark reaction chamber), algae seed input (expansion → dark reaction chamber), and algal liquid collection (dark reaction chamber → collection device).

[0047] Electrical connections link all actuators (pumps, valves, air conditioning) and sensors (temperature, concentration) to a central controller. The central controller (based on a PLC or industrial computer) integrates operating logic to achieve fully automated control, controlling the pneumatic diaphragm pump to maintain circulation flow. Based on distributed temperature sensor signals, it precisely controls the temperature (18-30℃±1℃) of the air conditioning and heat exchange coils. It controls the aeration rate and gas composition of the microporous aerators. Based on preset strategies or sensor feedback, it controls the diversion valve for algae replenishment. Based on concentration sensor signals, it controls the solenoid valve and collection pump for automated harvesting. Adjustable valves in the pretreatment unit control the wastewater input. This containerized design gives the system modularity, portability, rapid deployment, and supports standardized operation.

[0048] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A microalgae cultivation device integrated with a container, characterized by, include: A transparent, continuously curved U-shaped pipe with an inlet and an outlet; A bracket is used to secure the U-shaped pipe to the outer surface of the container. The dark reaction chamber is located inside the container and connected to the pneumatic diaphragm pump via a pipeline. The water inlet is connected to the dark reaction chamber via the pneumatic diaphragm pump, and the water outlet is directly connected to the dark reaction chamber, forming a closed loop system. The bracket fixes the U-shaped pipe to the outer surface of the container in a multi-layered stepped arrangement, forming a three-dimensional flow channel that starts from the first layer on the side of the container, extends through the first layer on the top surface to the second layer on the top surface, and finally returns to the second layer on the side of the container. The multi-layered stepped arrangement of the U-shaped pipes achieves flow channel connection through three-segment spatial turns. The U-shaped pipe at the end of the first layer on the side of the container is basically horizontally coplanar adjacent to the first layer on the top surface. The U-shaped pipe at the end of the first layer on the top surface is basically vertically coplanar adjacent to the second layer on the top surface. The U-shaped pipe at the end of the second layer on the top surface is basically vertically coplanar adjacent to the second layer on the side of the container. The support structure comprises two rows of parallel side supports and two rows of top supports, wherein: The lateral spacing between the two rows of side supports is equal to the center-to-center distance between the pipes of the first and second layers of the container side; the vertical spacing between the two rows of top supports is equal to the center-to-center distance between the pipes of the first and second layers of the top surface; the U-shaped pipe is detachably fixed to the preset anchor point of the support by clamps.

2. The microalgae cultivation device according to claim 1, characterized in that: It also includes a pretreatment unit located at the front of the container, the pretreatment unit including a funnel-shaped bottom water collection tank; the outlet of the water collection tank is connected to the wastewater inlet of the dark reaction tank through a diversion pipe, and the diversion pipe is equipped with an adjustable valve for controlling the wastewater input flow rate.

3. The microalgae cultivation device according to claim 2, characterized in that: It also includes a microalgae propagation device located inside the container, comprising at least two sets of vertically arranged transparent acrylic column reactors; the outlet of the microalgae propagation device is connected to the algae seed input port of the dark reaction chamber via a diversion valve, the diversion valve being able to switch between connected states to control the timing of algae seed delivery.

4. The microalgae cultivation device according to claim 3, characterized in that: It also includes a collection device located at the end of the container, which is connected to the bottom algae discharge port of the dark reaction tank via a pneumatic diaphragm pump; the inlet pipe of the collection device is connected in series with a solenoid valve and a concentration sensor, wherein: the concentration sensor monitors the algal biomass concentration in real time, and the solenoid valve opens the collection channel in response to the concentration signal.

5. The microalgae cultivation device according to claim 1, characterized in that: The dark reaction chamber is equipped with a temperature coordination control system. When the temperature sensor detects a value greater than 28°C, the air conditioner is activated to maintain the temperature inside the chamber within a gradient range of 18-30°C.

6. The microalgae cultivation device according to claim 1, characterized in that: The dark reaction chamber is equipped with a gas-liquid mixing device, including an annular gas distribution pipe and a microporous aerator connected thereto. The aerator has a pore diameter of 0.1-0.2 mm and a surface hydrophobic angle >150°.

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