A nucleic acid preservation card

Abstract

The utility model discloses a nucleic acid storage card relates to sample sampling device technical field, including card main part, be provided with dry storehouse and sample storage warehouse on the card main part, dry storehouse and sample storage warehouse are passed through gas passage intercommunication, be provided with dry block in dry storehouse. Sample is added in sample storage warehouse, and the water vapor in sample enters dry storehouse through gas passage and is absorbed by dry block, and the water vapor is absorbed quickly through setting dry block, realizes self -drying function, avoided the defect that traditional collection card class product must dry naturally and then save again, greatly promoted the convenience of the product in application.

Description

Technical Field

[0001] This utility model relates to the field of sample sampling device technology, specifically a nucleic acid preservation card. Background Technology

[0002] Nucleic acid testing plays a crucial role in many fields, including biomedical research, disease diagnosis, and epidemic prevention and control. Proper preservation of nucleic acid samples throughout the entire process, from collection to final testing, is of paramount importance. Nucleic acid preservation cards were developed in this context.

[0003] In the early days, traditional methods of preserving nucleic acid samples faced numerous challenges. Nucleic acids in samples such as blood, saliva, and tissue fluid are highly susceptible to degradation by nucleases, oxidants, and ultraviolet light under natural conditions. Furthermore, the growth of bacteria and other microorganisms accelerates nucleic acid degradation, making it difficult to preserve samples stably for extended periods. Especially during field sampling, testing in remote areas, and large-scale screening, traditional methods rely on cold chain transportation and low-temperature storage, which are demanding in terms of equipment, expensive, and inconvenient to operate, severely limiting the scope of nucleic acid testing.

[0004] In blood bank operations, the preservation of plasma (serum) samples has become a routine procedure. The revised "Blood Bank Management Regulations" of 2009 stipulate that blood samples should be preserved for two years after the use of blood components, thus providing a reliable basis for tracing blood test results. With the widespread adoption of nucleic acid testing, its results have long been the gold standard for diagnosis, but the preservation of RNA remains a challenge for the industry.

[0005] With the continuous advancement of technology, researchers have developed nucleic acid preservation cards through extensive research and experimentation. These cards are typically made of specially formulated filter paper, whose fibrous matrix is ​​soaked in a patented formula of powerful denaturing and chelating agents, containing special chemical substances. This ingenious design allows nucleic acids to be stably immobilized, protected from damage by nucleases, oxidants, and ultraviolet light, and effectively inhibits the growth of bacteria and other microorganisms, thereby maintaining the integrity of the nucleic acids.

[0006] However, in actual use, when the fibers come into contact with the filter paper, the drug solution undergoes a process of re-dissolving and drying. This process usually requires air drying at room temperature for more than 4 hours, which greatly increases the complexity of the operation and thus affects the user experience of the nucleic acid preservation card. Utility Model Content

[0007] This invention provides a nucleic acid preservation card to solve the problems mentioned in the background art.

[0008] To achieve the above objectives, this utility model provides the following technical solution:

[0009] A nucleic acid preservation card includes a card body, on which a drying chamber and a sample preservation chamber are disposed, the drying chamber and the sample preservation chamber are connected by a gas channel, and a drying block is disposed inside the drying chamber.

[0010] Preferably, the sample preservation chamber is provided with a sample dispersion pad and a nucleic acid preservation pad, with the sample dispersion pad located on top of the nucleic acid preservation pad.

[0011] Preferably, a cover plate is provided on the top of the card body, and the sample dispersion pad, nucleic acid preservation pad, drying block, drying chamber, gas channel and sample preservation chamber are covered under the cover plate.

[0012] Preferably, the cover plate is adhered to the side of the card body.

[0013] Preferably, the cover plate is provided with a sample dispensing hole, which is located above the sample dispersion pad.

[0014] Preferably, a sealing sticker is provided at the sample dispensing hole, the sealing sticker is adhered to the cover plate, and the sealing sticker is used to seal the sample dispensing hole.

[0015] Preferably, a handle is provided on the sealing edge side, and the handle on the sealing edge side is separate from the cover plate.

[0016] Preferably, the card body is made of plastic and integrally molded by injection molding.

[0017] Preferably, the dried block comprises cellulose and at least one of calcium chloride, magnesium chloride, and calcium oxide.

[0018] Preferably, the sample dispersion pad is made of a hydrophilic porous material, the average pore size of the sample dispersion pad material is 1-50 micrometers, and the thickness of the sample dispersion pad material is 0.1-0.5 millimeters.

[0019] Compared with the prior art, the present invention has at least the following beneficial effects:

[0020] The sample is added to the sample preservation chamber. The water vapor in the sample enters the drying chamber through the gas channel and is absorbed by the drying block. By setting the drying block to quickly absorb water vapor, the self-drying function is realized, avoiding the defect of traditional data acquisition card products that must be naturally dried before preservation, and greatly improving the convenience of this product in application. Attached Figure Description

[0021] Figure 1 This is an exploded view of the main structure of this utility model;

[0022] Figure 2 This is a schematic diagram of the main structure of this utility model;

[0023] Figure 3This is a schematic diagram of the internal structure of the card body of this utility model;

[0024] Figure 4 This is a schematic diagram of the gas channel structure of this utility model.

[0025] In the diagram: 1. Card body; 2. Cover plate; 3. Sealing sticker; 4. Sample dispensing hole; 5. Sample dispersion pad; 6. Nucleic acid preservation pad; 7. Drying block; 8. Drying chamber; 9. Gas channel; 10. Sample preservation chamber. Detailed Implementation

[0026] In this utility model, the terms "first," "second," etc., are used for descriptive purposes only and do not specifically refer to any order or sequence, nor are they intended to limit the utility model. They are merely used to distinguish protective components or operations described with the same technical terms, and should not be construed as indicating or implying their relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include at least one of that feature. Furthermore, the technical solutions and features of the various embodiments can be combined with each other, but this must be based on the ability of a person skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0027] Example

[0028] Please see Figures 1-4 A nucleic acid preservation card includes a card body 1, on which a drying chamber 8 and a sample preservation chamber 10 are provided. The drying chamber 8 and the sample preservation chamber 10 are connected through a gas channel 9. A drying block 7 is provided inside the drying chamber 8.

[0029] The card body 1 is made of plastic material and is integrally molded by injection molding, thereby forming a structure in which the drying chamber 8 and the sample preservation chamber 10 are separated by gas channels 9. Multiple sets of gas channels 9 are arranged in parallel so that water vapor in the sample enters the drying chamber 8 through the gas channels 9 and is absorbed by the drying block 7.

[0030] The sample storage chamber 10 is provided with a sample dispersion pad 5 and a nucleic acid storage pad 6, with the sample dispersion pad 5 located on the upper side of the nucleic acid storage pad 6.

[0031] The card body 1 is provided with a cover plate 2 on top, and the cover plate 2 covers a sample dispersion pad 5, a nucleic acid preservation pad 6, a drying block 7, a drying chamber 8, a gas channel 9, and a sample preservation chamber 10.

[0032] The cover plate 2 can be made of PVC board with adhesive or other plastic adhesive material, and the cover plate 2 is glued to the card body 1 with adhesive.

[0033] The cover plate 2 is attached to the side of the card body 1. Inside the side of the card body 1 are a drying chamber 8, a gas channel 9, and a sample preservation chamber 10. The cover plate 2 is used to maintain the airtightness of the sample dispersion pad 5, nucleic acid preservation pad 6, drying block 7, drying chamber 8, gas channel 9, sample preservation chamber 10, and sample inside the card body 1.

[0034] The cover plate 2 is provided with a sample addition hole 4, which is located above the sample dispersion pad 5. The sample addition hole 4 is used to add samples, which is quick and convenient.

[0035] A sealing sticker 3 is provided at the sample dispensing hole 4. The sealing sticker 3 is adhered to the cover plate 2 by a waterproof adhesive sticker. The sealing sticker 3 is used to seal the sample dispensing hole 4. When dispensing the sample, the sealing sticker 3 is peeled off to expose the sample dispensing hole 4 and then the sample can be dispensed.

[0036] The sealing sticker 3 has a handle on its side. The handle on the side of the sealing sticker 3 is separate from the cover plate 2. No waterproof adhesive sticker is placed between the handle and the cover plate 2. The waterproof adhesive sticker is placed on the side of the connection between the handle and the sealing sticker 3, close to the sealing sticker 3.

[0037] The dried block 7 comprises cellulose and at least one of calcium chloride, magnesium chloride, and calcium oxide.

[0038] The sample dispersion pad 5 is made of a hydrophilic porous material with an average pore size of 1-50 micrometers and a thickness of 0.1-0.5 millimeters.

[0039] The method of using the nucleic acid preservation card is as follows:

[0040] Place the nucleic acid preservation card on a horizontal table, peel off the sealing sticker 3 to expose the sample application well 4, and accurately aspirate 500 μL of sample from the sample application well 4 into the sample application area of ​​the nucleic acid preservation card. The sample application volume of each application unit shall not exceed 500 μL.

[0041] After the sample has been fully absorbed, apply the sealing sticker 3 and it is ready for transportation and storage.

[0042] The drying ability of the nucleic acid preservation cards was tested as follows:

[0043] Pure water was used as a simulated sample, and 500 μl of pure water was added dropwise to the nucleic acid preservation card (500 μl of pure water weighs 0.5 g). After sealing for 72 hours, the weight was measured, and the overall mass difference between the sample dispersion pad 5 and the nucleic acid preservation pad 6 before and after sample addition was calculated, and the drying efficiency was calculated.

[0044] Nucleic Acid Preservation Card Drying Efficiency Table

[0045] Card 1 3.721 3.236 97.0％ Card 2 3.717 3.238 95.8％ Card 3 3.745 3.263 98.2％

[0046] The working principle and beneficial effects of the above scheme are as follows:

[0047] The sample is added to the sample dispersion pad 5 and the nucleic acid preservation pad 6 to increase the evaporation area of ​​the sample. The water vapor in the sample enters the drying chamber 8 through the gas channel 9 and is absorbed by the drying block 7. By setting the drying block 7 to quickly absorb water vapor, the self-drying function is realized, avoiding the defect of traditional collection card products that must be naturally dried before storage, and greatly improving the convenience of this product in application.

[0048] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A nucleic acid preservation card, characterized in that, Includes a card body (1), on which a drying chamber (8) and a sample preservation chamber (10) are provided. The drying chamber (8) and the sample preservation chamber (10) are connected through a gas channel (9). A drying block (7) is provided inside the drying chamber (8).

2. The nucleic acid preservation card according to claim 1, characterized in that, The sample storage chamber (10) is equipped with a sample dispersion pad (5) and a nucleic acid storage pad (6), with the sample dispersion pad (5) located on the upper side of the nucleic acid storage pad (6).

3. A nucleic acid preservation card according to claim 2, characterized in that, The card body (1) is provided with a cover plate (2) on top, and the cover plate (2) covers a sample dispersion pad (5), a nucleic acid preservation pad (6), a drying block (7), a drying chamber (8), a gas channel (9) and a sample preservation chamber (10).

4. A nucleic acid preservation card according to claim 3, characterized in that, The cover plate (2) is attached to the side of the card body (1).

5. A nucleic acid preservation card according to claim 4, characterized in that, The cover plate (2) is provided with a sample addition hole (4), which is located above the sample dispersion pad (5).

6. A nucleic acid preservation card according to claim 5, characterized in that, A sealing sticker (3) is provided at the sample feeding hole (4). The sealing sticker (3) is attached to the cover plate (2) and is used to seal the sample feeding hole (4).

7. A nucleic acid preservation card according to claim 6, characterized in that, A handle is provided on the side of the sealing sticker (3), and the handle on the side of the sealing sticker (3) is separated from the cover plate (2).

8. A nucleic acid preservation card according to claim 1, characterized in that, The card body (1) is made of plastic material and is integrally molded by injection molding.

9. A nucleic acid preservation card according to claim 2, characterized in that, The sample dispersion pad (5) is made of a hydrophilic porous material. The average pore size of the sample dispersion pad (5) material is 1-50 micrometers, and the thickness of the sample dispersion pad (5) material is 0.1-0.5 millimeters.

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